• We herein report that oocyte-specific Uhrf1 KO results in impaired chromosome segregation, abnormal cleavage division, and preimplantation lethality of derived embryos. (life-science-alliance.org)
  • Instytut Genetyki i Hodowli Zwierząt PAN zaprasza na sympozjum naukowe „From oocyte to embryo - dynamics of preimplantation mammalian development" w dniu 17.09.2019, w siedzibie Instytutu. (igbzpan.pl)
  • We are also interested in how variations in DNA methylation come about in oocytes and whether we can use this variation as a marker for oocyte quality and embryo potential. (babraham.ac.uk)
  • During pre-implantation stages of mammalian development, maternally stored material promotes both the erasure of the sperm and oocyte epigenetic profiles and is responsible for concomitant genome activation. (babraham.ac.uk)
  • In 1-cell and 2-cell embryos Dnmt1s is derived from the oocyte, whereas from the 2-cell stage onward the embryo starts to synthesize its own Dnmt1s 8 . (nature.com)
  • We have shown previously that this change in cortex mechanics controls the geometry of oocyte (and embryo) division since oocytes presenting too stiff or too soft cortices divide symmetrically (1-3). (espci.fr)
  • We show that aberrant cortical tension, a frequent defect in a normal population, could lead to chromosome alignment defects in oocytes, potentially contributing to oocyte predisposition to chromosome segregation defects, a leading cause of aneuploidy in embryos. (espci.fr)
  • Through single-cell RNA-sequencing (RNA-seq), we report here a comprehensive analysis of transcriptome dynamics from oocyte to morula in both human and mouse embryos. (uth.edu)
  • The production of CO2 from glucose by sheep embryos has been determined[ 3 ] and results have indicated that little glucose is utilized by the embryos of sheep and that blastocysts derived from oocytes, matured and fertilized in vitro , oxidized glucose at a lower rate than those developed in vivo . (biomedcentral.com)
  • However, UHRF1 is predominantly localized in the cytoplasm of mouse oocytes and preimplantation embryos, where it may play a role unrelated to the nuclear function. (life-science-alliance.org)
  • Thus, maternal UHRF1 regulates the proper cytoplasmic architecture and function of oocytes and preimplantation embryos, likely through a mechanism unrelated to DNA methylation. (life-science-alliance.org)
  • We examine how epigenetic states are set up in oocytes - or egg cells - and influence gene expression in the embryo. (babraham.ac.uk)
  • Here, we have utilized single-cell methylome and transcriptome sequencing (scM&T-seq) to quantify both mRNA expression and DNA methylation in oocytes and a developmental series of human embryos at single-cell resolution. (babraham.ac.uk)
  • Interestingly, human and mouse oocytes developmental potential is accurately predicted by their cortical tension within hours after fertilization: if they are too stiff or too soft, embryos will cease development (4). (espci.fr)
  • The blastocoel is a crucial component of amphibian embryo development. (wikipedia.org)
  • To determine the role of PIF in post-fertilization embryo development, we measured the peptide's concentration in the culture medium and tested endogenous PIF's potential trophic effects and direct interaction with the embryo. (biomedcentral.com)
  • Examine the inhibitory effects of anti-PIF-monoclonal antibody (mAb) added to medium on cultured mouse embryos development. (biomedcentral.com)
  • PIF is an early embryo viability marker that has a direct supportive role on embryo development in culture. (biomedcentral.com)
  • Overall, our data supports PIF's endogenous self sustaining role in embryo development and the utility of PIF- ELISA to detect viable embryos in a non-invasive manner. (biomedcentral.com)
  • Nop2 is required for mammalian preimplantation development. (umass.edu)
  • The expression pattern and function of Nop2 during early mammalian embryo development, however, has not been investigated. (umass.edu)
  • We identified Nop2 as an essential gene for development to the blastocyst stage while performing an RNA interference (RNAi)-based screen in mouse preimplantation embryos. (umass.edu)
  • Nop2 is expressed throughout preimplantation development, with highest mRNA and protein accumulation at the 8-cell and morula stages, respectively. (umass.edu)
  • Taken together, our results demonstrate that Nop2 is an essential gene for blastocyst formation, and is required for RNA processing and/or stability in vivo during preimplantation embryo development in the mouse. (umass.edu)
  • Live dynamic analysis of cardiac development and congenital defects in mouse embryos. (bcm.edu)
  • Stewart CL: LIF and the regulation of the preimplantation development of the mammalian embryo. (spandidos-publications.com)
  • Recent advances in live imaging and genetics of mammalian division, movement and cell differentiation leading to development which integrate observations of biochemical tissue formation [14 ]. (lu.se)
  • Surgical embryo recovery was performed on Days 4 and 6 after onset of estrus (Day 0) and recovered embryos were subjected to comparative metabolism studies with in vitro derived embryos at the same stage of development. (biomedcentral.com)
  • The first lineage specification during mammalian embryo development can be visually distinguished at the blastocyst stage. (bioone.org)
  • Also, I like to work on my own little project, which is focused on discovering similarities and differences in early embryonic development of different species of mammalian pre-implantation embryos. (biologists.com)
  • 3. Takenaka M., Horiuchi T., Yanagimachi R. Effects of light on development of mammalian zygotes. (medline.ru)
  • My laboratory has developed new technologies and applied them towards understanding the molecular biology and enzymology of DNA replication in animal cells and viruses (SV40, polyomavirus, papillomavirus, and herpes simplex virus), and at the beginning of animal development (mouse preimplantation embryos and frog eggs). (nih.gov)
  • 2) What are the requirements for DNA replication and transcription at the beginning of mammalian development? (nih.gov)
  • When nonhuman mammalian development is compared with human development, the study subjects must be compared at the same developmental stage (fetal, perinatal, postnatal) When collected appropriately, data from experimental studies of nonhuman mammalian embryos elucidate important aspects of human facial development. (medscape.com)
  • The bovine embryos with higher oxygen consumption are better candidates to further development into good quality embryos and yielded higher pregnancy rates after embryo transfer. (bioone.org)
  • This new conference will focus on discoveries and innovative technologies to study the origin of life, from gametes to embryo development to stem cells. (genetics.org.uk)
  • At an individual level, the origin and progression of mammalian development and its implications for human health concerns us all. (genetics.org.uk)
  • The programme will offer an interchange of ideas across the fields of mammalian stem cell biology, embryo development and human reproduction. (genetics.org.uk)
  • Furthermore, due to ethical and legal concerns, very limited techniques can be applied to human embryos to validate some of significant conclusions drawn from descriptive studies regarding human embryonic development. (nature.com)
  • Researchers at the Francis Crick Institute provide fundamental insights into early human development by single-cell RNA-sequencing of human and mouse preimplantation embryos. (rna-seqblog.com)
  • Altogether, our results demonstrate that the expression of zc3h12a must be tightly controlled during the first cell divisions of zebrafish embryos and that a rapid decrease in its mRNA expression is an important factor promoting proper embryo development. (bvsalud.org)
  • Mammalian preimplantation development is a complex process involving dramatic changes in the transcriptional architecture. (uth.edu)
  • These genes represent novel candidates that are likely key players in driving mammalian preimplantation development. (uth.edu)
  • Collectively, we demonstrate that mammalian preimplantation development is orchestrated by evolutionarily conserved genetic programs that diverge in developmental timing. (uth.edu)
  • Our results provide a valuable resource to dissect gene regulatory mechanism underlying progressive development of early mammalian embryos. (uth.edu)
  • Here, we consider the metabolism of the early embryo through development, and look at the nutrient milieu within the developing stem cell niche. (conditionmed.org)
  • The developing embryo, from which pluripotent stem cells originate, undergoes a series of dynamic metabolic transitions synchronized to its molecular development. (conditionmed.org)
  • The roles of hormones and growth factors during the early stages of mammalian development are poorly understood. (nebraska.edu)
  • During the past decade, it has become apparent that embryos at several stages of development produce growth factors, some of which exhibit the properties of transforming growth factors (TGFs). (nebraska.edu)
  • The work proposed in this application will help to clarify the roles of growth factors during early development and help determine whether further study of embryonic growth factors is likely to yield assays suitable for monitoring normal preimplantation development. (nebraska.edu)
  • Braude P., Bolton V., Moore S., Human gene expression first occurs between the four- , and eight-cell stages of preimplantation development. (biologie-journal.org)
  • Howlett S.K., Reik W., Methylation levels of maternal , and paternal genomes during preimplantation development. (biologie-journal.org)
  • After intracytoplasmic sperm injection (ICSI), 48 embryos were evaluated on day 3 of their development, according to their cell number. (who.int)
  • In vivo and in organized cells, and proper symmetry are healthy individuals, macrophages can characteristics of higher-quality embryos, which phagocytize DNA that has been passively point to healthy development and higher rates of released into the blood from apoptotic or necrotic implantation. (who.int)
  • The synchronization and ovulatory responses of Sangsari cross bred ewes and metabolism of energy substrates in 8-cell stage embryos to hatched blastocysts stage produced in vitro or in vivo were investigated. (biomedcentral.com)
  • Here we report genome-wide composition, patterning, and stage-specific dynamics of DNA methylation in pre-implantation rhesus monkey embryos as well as male and female gametes studied using an optimized tagmentation-based whole-genome bisulfite sequencing method. (nature.com)
  • During preimplantation stages, differentiation occurs between precursors of embryonic and extraembryonic structures. (medscape.com)
  • Determine PIF levels in culture medium of multiple mouse and single bovine embryos cultured up to the blastocyst stage using PIF-ELISA. (biomedcentral.com)
  • In mouse embryos, cells seem prepatterned to become certain cell lineage because the first cleavage plane has been related with further embryonic-abembryonic axis at the blastocyst stage. (bioone.org)
  • To achieve this, cells of in vitro produced bovine embryos were traced from the 2-cell stage to the blastocyst stage. (bioone.org)
  • Recently, we have determined that embryos cultured from the blastocyst stage release growth factors that induce the soft agar growth of NRK cells. (nebraska.edu)
  • Assessing equine embryo developmental competency by time-lapse image analysis. (ohsu.edu)
  • Time-Lapse Imaging for the Detection of Chromosomal Abnormalities in Primate Preimplantation Embryos. (ohsu.edu)
  • By comparing these signatures with early embryos that have undergone spontaneous cleavage-stage arrest, as determined by time-lapse imaging, we identify embryos that fail to appropriately activate their genomes or undergo epigenetic reprogramming. (babraham.ac.uk)
  • Current use of three-dimensional surfaces instead of large flat bottoms is restricted to time-lapse systems, but these wells are designed for optical clarity, not for the needs of embryos. (msu.ru)
  • The vegetal plate and animal hemisphere develop and secrete a hatching enzyme that digests the fertilization envelope and allows the embryo to now become a free-swimming hatched blastula. (wikipedia.org)
  • Hasegawa J, Iwasaki S, Yanaihara A, Negishi M, Tahara R and Okai T: Correlations between steroids concentration in follicular fluid, pronuclear morphology and embryo qualities in vitro fertilization. (spandidos-publications.com)
  • SECM technique may be a valuable tool for accurately assessing the quality of embryos and thereby contribute to improving outcomes associated with assisted reproduction, including human in vitro fertilization. (bioone.org)
  • Adenot P.G., Szollosi M.S., Geze M., Renard J.P., Debey P., Dynamics of paternal chromatin changes in live one-cell mouse embryo after natural fertilization. (biologie-journal.org)
  • Haaf T., The battle of the sexes after fertilization: behaviour of paternal and maternal chromosomes in the early mammalian embryo. (biologie-journal.org)
  • Huang J.C., Lei Z.L., Shi L.H., Miao Y.L., Yang J.W., Ouyang Y.C., Sun Q.Y., Chen D.Y., Comparison of histone modifications in in vivo and in vitro fertilization mouse embryos. (biologie-journal.org)
  • High-resolution imaging of multiple embryos at the same time can be compromised by embryos displacement during imaging. (pasteur.fr)
  • Superovulation ensures recovery of multiple embryos during one estrous cycle, thus allowing us to maximally exploit the female germ pool. (biomedcentral.com)
  • At the 120- cell stage, the sea urchin embryo is considered a blastula because of its developed blastocoel, which every embryonic cell surrounds and touches. (wikipedia.org)
  • The early mammalian embryo consists of the extra-embryonic cell layers-the trophoblast and a body of cells called the inner cell mass (ICM), which eventually become the embryo proper. (thefutureofthings.com)
  • We fully characterize embryonic genome activation and maternal transcript degradation and map key epigenetic reprogramming events in developmentally high-quality embryos. (babraham.ac.uk)
  • We further found that ectopic overexpression of wild-type Mcpip1 but not the catalytically inactive mutant form resulted in an embryonic lethal phenotype in zebrafish embryos (24 hpf). (bvsalud.org)
  • Our results indicate that a failure to successfully accomplish these essential milestones impedes the developmental potential of pre-implantation embryos and is likely to have important implications, similar to aneuploidy, for the success of assisted reproductive cycles. (babraham.ac.uk)
  • Although the precise developmental stage when production of these factors begins has not been established, the data suggest that these factors are released by cell types associated with early postimplantation embryos. (nebraska.edu)
  • Although the genome-wide DNA demethylation is believed to be a hallmark of mammalian embryogenesis, previous study also indicated that the somatic form of dnmt1 ( dnmt1s ) is actually expressed at each stage of pre-implantation embryos and plays a role in the maintenance of DNA imprinting 8 . (nature.com)
  • NOP2-deficient embryos exhibit reduced blastomere numbers, greatly increased apoptosis, and impaired cell-lineage specification. (umass.edu)
  • Single-cell sequencing of primate preimplantation embryos reveals chromosome elimination via cellular fragmentation and blastomere exclusion. (ohsu.edu)
  • While the pattern of oxidation was similar among in vitro and in vivo derived embryos, a low pyruvate to lactate ratio was the preferred substrate of embryos derived in vitro . (biomedcentral.com)
  • Respiration activity correlates with the embryo quality. (bioone.org)
  • It was demonstrated in the frog embryo that the first cleavage furrow widens in the animal hemisphere creating a small intercellular cavity that is sealed off via tight junctions. (wikipedia.org)
  • Understanding the association between Cell-free DNA levels in embryo CM and the quality of embryo cleavage could help improve the quality of IVF techniques. (who.int)
  • This prospective study was conducted with 96 spent CM from patients undergoing IVF cycle, in order to determine relationships of Cell-free DNA levels in embryo CM with embryo cleavage quality on day 3. (who.int)
  • We conclude that cel -free DNA levels in CM might be associated with delayed embryo cleavage. (who.int)
  • The TGF-like growth factors from embryos were initially observed in extracts prepared from mouse embryos at mid and late stages of gestation. (nebraska.edu)
  • Rieger[ 4 ] inferred that the pre-implantation bovine embryo undergoes an intensified period of cellular synthetic processes which require appropriate substrates for production of energy and reducing equivalents in the form of nicotinamide-adenine dinucleotide phosphate. (biomedcentral.com)
  • Kopecny V., Fléchon J.E., Camous S., Fulka J. Jr., Nucleologenesis , and the onset of transcription in the eight-cell bovine embryo: fine-structural autoradiographic study. (biologie-journal.org)
  • Detectable - though low - PIF levels were secreted already by 2-cell stage mouse embryos. (biomedcentral.com)
  • Our approaches combine live embryo manipulation protocols, mouse models of congenital defects, state-of-the-art functional optical coherence tomography technology, confocal microscopy and advanced computational analysis. (bcm.edu)
  • The mouse preimplantation embryo is an excellent system for studying how mammalian cells organize dynamically into increasingly complex structures. (pasteur.fr)
  • In this chapter, we describe the different steps of production and storage of the imaging device as well as its use for live imaging of mouse preimplantation embryos expressing fluorescent reporters from genetically modified alleles or after in vitro transcribed mRNA transfer by microinjection or electroporation. (pasteur.fr)
  • In the mouse, a transporting epithelium is established around the 8-16-cell stage through a process known as compaction where cell definition is lost and the outer cells of the embryo form tight junctions, giving rise to the blastocyst. (conditionmed.org)
  • This application proposes to address several important questions concerning TGF-like growth factors released by early mouse embryos: 1) What are their identities? (nebraska.edu)
  • Adenot P.G., Mercier Y., Renard J.P., Thompson E.M., Differential H4 acetylation of paternal, maternal chromatin precedes DNA replication, and differential transcriptional activity in pronuclei of 1-cell mouse embryos. (biologie-journal.org)
  • Aoki F., Worrad D.M., Schultz R.M., Regulation of transcriptional activity during the first , and second cell cycles in the preimplantation mouse embryo. (biologie-journal.org)
  • Bouniol C., Nguyen E., Debey P., Endogenous transcription occurs at the 1-cell stage in the mouse embryo. (biologie-journal.org)
  • A comprehensive landscape of chromatin states for multiple mammalian tissues is essential for elucidating the molecular mechanism underlying regulatory variants on complex traits. (biomedcentral.com)
  • Fulka H., St John J.C., Fulka J., Hozak P., Chromatin in early mammalian embryos: achieving the pluripotent state. (biologie-journal.org)
  • Giraldo A.M., Hylan D.A., Ballard C.B., Purpera M.N., Vaught T.D., Lynn J.W., Godke R.A., Bondioli K.R., Effect of epigenetic modifications of donor somatic cells on the subsequent chromatin remodeling of cloned bovine embryos. (biologie-journal.org)
  • Estrogen-related receptors (ERRs) are transcriptional regulatory factors that play crucial roles in mammalian energy storage and expenditure. (bvsalud.org)
  • The search to identify embryo-specific markers which reflect viability of cultured embryos by assessing the medium or by performing an embryo biopsy (beyond morphological evaluation) has been ongoing. (biomedcentral.com)
  • Measurement of free radicals in the culture medium of developing embryos has been reported as an index for embryo viability. (biomedcentral.com)
  • 5. Oh S.J., Gong S.P., Lee, S.T. Light intensity and wavelength during embryo manipulation are important factors for maintaining viability of preimplantation embryos in vitro. (medline.ru)
  • 8. Oh S.J., Gong S.P., Lee, S.T. Light intensity and wavelength during embryo manipulation are important factors for maintaining viability of preimplantation embryos in vitro. (medline.ru)
  • Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)
  • In the past, our research focused on viral genomes as models for DNA replication in mammalian cell nuclei. (nih.gov)
  • Chromosomal instability in mammalian pre-implantation embryos: potential causes, detection methods, and clinical consequences. (ohsu.edu)
  • Mammalian pre-implantation chromosomal instability: species comparison, evolutionary considerations, and pathological correlations. (nih.gov)
  • In our lab, we seek to understand how the uterine microenvironment shapes the fetal-placental interface of various animals, by using early embryos and placental organoids. (biologists.com)
  • The visual input was always tremendously important for me and it still gives me enormous pleasure to look down a microscope at embryos and tissues and wonder how they develop. (biologists.com)
  • Bioethics in human embryology: the double-edged sword of embryo research. (nih.gov)
  • The moment the human embryo is fertilized to the week of the baby's birth is an important period for human appearance in the normally developing embryo. (medscape.com)
  • SECM measuring system can non-invasively measure respiration activity by single embryos of several species including human. (bioone.org)
  • Single-cell multi-omic analysis profiles defective genome activation and epigenetic reprogramming associated with human pre-implantation embryo arrest. (babraham.ac.uk)
  • The meeting will highlight the power of stem cells and mammalian models to understand human reproduction and the arising biomedical opportunities. (genetics.org.uk)
  • Simultaneous assessment of aneuploidy, polymorphisms, and mitochondrial DNA content in human polar bodies and embryos with the use of a novel microarray platform. (takarabio.com)
  • Although in vitro culture of human embryos is a crucial step in assisted reproduction, the lack of focused research hampers worldwide standardisation and consistent outcomes. (msu.ru)
  • In single bovine IVF-derived embryos, PIF levels in medium at day 3 of culture were higher than non-cleaving embryos (control) (P = 0.01) and at day 7 were higher than day 3 (P = 0.03). (biomedcentral.com)
  • In this paper, we describe a scanning electrochemical microscopy (SECM) technique that is a non-invasive and sensitive method for measuring oxygen consumption by individual embryos. (bioone.org)
  • Since the immune milieu of pregnancy is unique - not replicated in any other circumstances - specific embryo-derived signals have a crucial role leading to maternal recognition of pregnancy [ 4 ]. (biomedcentral.com)
  • It is the first cell cavity formed as the embryo enlarges, and is the essential precursor for the differentiated gastrula. (wikipedia.org)
  • The inner cell mass will go on to become the actual embryo. (wikipedia.org)
  • As the embryo further divides, the blastocoel expands and the inner cell mass is positioned on one side of the trophoblast cells forming a mammalian blastula, called a blastocyst. (wikipedia.org)
  • An amphibian embryo in the 128- cell stage is considered a blastula as the blastocoel in the embryo becomes apparent during this stage. (wikipedia.org)
  • 6. Schumacher A., Fischer B. Influence of visible light and room temperature on cell proliferation in preimplantation rabbit embryos. (medline.ru)
  • 2019). Akin to the dynamic nutrient requirements of the developing embryo, discrete in vitro cell states have distinct metabolic profiles (Zhou et al. (conditionmed.org)
  • Here are the latest publications comparing PicoPLEX technology to other single-cell whole-genome amplification technologies, and using PicoPLEX technology to uncover insights from CTCs, single cells from FFPE tumor samples, and samples relevant to preimplantation genetic testing. (takarabio.com)
  • Day 2 and day 3 CM corresponding to each one of the embryos was analyzed, by quantitative PCR, for estimation of Cell-free DNA levels. (who.int)
  • The results revealed a significant increase in Cell-free DNA levels on day 2 CM corresponding to 4 to 6 cell embryos compared to those corresponding to 7 to 8 cel embryos (p=0.04). (who.int)
  • As for day 3 CM, the results showed no significant difference between the Cell-Free DNA levels in CM of 7-8 and those of 4-6 cell embryos (p=0.4). (who.int)
  • Researchers have developed a novel imaging approach that has allowed them to see eggs and embryos as they move along the fallopian tube in a live animal. (bcm.edu)
  • Our objective was to determine whether this would be the case in another mammalian species, the bovine. (bioone.org)
  • Browne RW, Bloom MS, Shelly WB, Ocque AJ, Huddleston HG and Fujimoto VY: Follicular fluid high density lipoprotein-associated micronutrient levels are associated with embryo fragmentation during IVF. (spandidos-publications.com)
  • The conference will also include discussions on invasive and non-invasive techniques for both preimplantation selection and genetic diagnosis. (genetics.org.uk)
  • The ubiquitin-proteasome system performs various regulatory and homeostatic roles in mammalian copy. (aabioetica.org)
  • the embryo is still a ball of dividing cells. (wikipedia.org)
  • Surprisingly, we found that there is a significant percentage of the embryos (∼60%) with labeled and nonlabeled cells randomly distributed and intermingled. (bioone.org)
  • Our current research now focuses on two basic, interrelated questions: (1) How do mammalian cells decide where and when to initiate DNA replication? (nih.gov)
  • Failure of mammalian cells to regulate their proliferation cycle leads to cancer. (nih.gov)
  • Low-quality embryos, on the other cells, thereby maintaining a relatively low basal hand, frequently display morphological level [16-18]. (who.int)
  • Bellier S., Chastant S., Adenot P., Vincent M., Renard J.P., Bensaude O., Nuclear translocation , and carboxyl-terminal domain phosphorylation of RNA polymerase II delineate the two phases of zygotic gene activation in mammalian embryos. (biologie-journal.org)
  • Abdalla H., Yoshizawa Y., Hochi S., Active demethylation of paternal genome in mammalian zygotes. (biologie-journal.org)
  • Schumacher A., Fisher B. Effects of visible light and room temperature on the ultrastructure of preimplantation rabbit embryos: a time course study. (medline.ru)