Mammalian chromosomes hybrid cells. Medical search. Frequent questions

Radiation hybrid mapping was also used in constructing early physical maps of the human genome. (wikipedia.org)
Irina began her career in academia at the Danish Academy of Sciences, Denmark researching mammalian genome mapping and at the University of Iowa Hospitals and Clinics, heading the cross-departmental functional genomics team focused on biomarker discovery to understand disease mechanisms and discover new drug targets. (sachibio.com)
Finally, this RH map was used for testing the accuracy of the chicken genome assembly for chromosome 5. (biomedcentral.com)
The high resolution framework map obtained in this study has markers covering the entire chicken chromosome 5 and reveals the existence of a high number of rearrangements when compared to the human genome. (biomedcentral.com)
We suggested that the genome undergoes specific imprinting during gametogenesis 3 and that some paternal genes may be necessary for normal development of the extraembryonic tissues 3,4 , in which only the maternal X chromosome remains active 5-9 . (nature.com)
HIV-1 belongs to the Lentiviridae genus of retroviruses and its replication depends on the integration of the reverse-transcribed viral genome into the host chromosome. (biomedcentral.com)
Using a computational approach for haplotype-resolved Hi-C, the authors reveal highly structured homolog pairing in Drosophila embryos during zygotic genome activation and demonstrate its application to mammalian embryos. (homologyeffects.org)
Here, the authors use haplotype-resolved Hi-C to identify genome-wide trans -homolog interactions in a Drosophila hybrid cell line and investigate complex types of pairing and functional roles. (homologyeffects.org)
Genome instability is a feature of cancer cells, transcription being an important source of DNA damage. (us.es)
Unscheduled R loops can be a source of genome instability, a hallmark of cancer cells. (us.es)
2005). A comprehensive radiation hybrid map of the bovine genome comprising 5593 loci. (geneticsmr.com)
Mammalian Genome 10:176-181. (uh.edu)

Primer sets for 21 genes yielded eight horse-specific markers, which were mapped with the UC Davis horse-mouse somatic cell hybrid panel into two synteny groups: UCD14 and UCD21. (etsu.edu)

Chromatin conformation and stem cell differentiation were minimally affected. (bvsalud.org)
Chromatin binding assays in yeast and mammalian cells demonstrated that these IN mutants were impaired for the ability to bind chromatin. (biomedcentral.com)
2009 ). At a larger scale, DSB formation occurs in the context of a specific chromosome architecture that consists of chromatin loops anchored to a proteinaceous axis (Zickler and Kleckner 1999 ). (springer.com)
DSB sites are preferentially located within chromatin loops, while several proteins that are required for DSB formation (Rec114, Mei4, and Mer2) localize on the chromosome axis (Blat et al. (springer.com)
1998). Genetic dissection of SLE pathogenesis: Sle1 on murine chromosome 1 leads to selective loss of tolerance to chromatin components. (uh.edu)

Chromosome engineering has been attempted successfully in yeast but remains challenging in higher eukaryotes, including mammals. (bvsalud.org)
In addition, we identified proteins that interact with the KRAB domain of PRDM9 in yeast two-hybrid assay screens, particularly CXXC1, a member of the COMPASS complex. (springer.com)

Constitutive expression of CD69 in interspecies T-cell hybrids and locus assignment to human chromosome 12. (unige.ch)
An interstitial deletion of the pseudoautosomal region 1 (PAR1) of either of the sex chromosomes results in juxtaposition of the first noncoding exon of the P2RY8 gene to the first exon of CRLF2, such that CRLF2 expression from this chimeric locus is driven by the P2RY8 promoter. (atlasgeneticsoncology.org)
A tumor suppressor gene (GENES, TUMOR SUPPRESSOR) located on human CHROMOSOME 17 at locus 17q21. (lookformedical.com)
A tumor suppressor gene (GENES, TUMOR SUPPRESSOR) located on human chromosome 13 at locus 13q12.3. (lookformedical.com)

The resolution of radiation hybrid (RH) maps is intermediate between that of the genetic and BAC (Bacterial Artificial Chromosome) contig maps. (biomedcentral.com)
Radiation hybrid maps have a resolution power intermediate to that of the genetic and BAC contig maps and are also a powerful tool for the mapping of ESTs and genes by simple PCR. (biomedcentral.com)
This information was used to develop markers from chicken EST sequence data orthologous to genes in these human regions, in addition to the existing markers from the chicken chromosome 5 genetic map. (biomedcentral.com)
In molecular biology, a vector is an entity that is used to transfer the genetic material , such as a plasmid that is used to transfer DNA sequences from the donor organism to the target cell of the recipient organism. (biologyonline.com)
In micro and molecular biology, a vector is a method of transportation or it may serve as an agent that transports a piece of genetic material into a cell or organism. (biologyonline.com)
In addition to basic science investigations in such areas as tumor cell invasion, oncogenes, immunotherapy and genetic inheritance, our scientists and physicians participate in national cancer trials. (upstate.edu)
During the creation of a chimera, the DNA molecules do not exchange genetic material (recombine), unlike in sexual reproduction or in hybrid organisms, which result from genetic material exchanged between two different species. (asu.edu)
1997). Genetic dissection of SLE pathogenesis : Sle2 on murine chromosome 4 leads to B-cell hyperactivity. (uh.edu)
Genetic dissection of SLE pathogenesis: Sle3 impacts T-cell activation, differentiation and cell death. (uh.edu)
1999). Genetic dissection of SLE pathogenesis: adoptive transfer of Sle1 on murine chromosome 1 by bone marrow. (uh.edu)
Genetic dissection of lupus: T-cell hyperactivity as a consequence of hyperstimulatory antigen presenting cells. (uh.edu)
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. (lookformedical.com)

Improved methods and pharmaceutical compositions are provided herein for mobilizing hematopoietic progenitor cells from bone marrow into peripheral blood, comprising the administration of an effective amount of an inhibitor of GTPases, such as Rac1 and Rac2 alone or in combination. (justia.com)
Therefore, hematopoietic stem cells mobilized into a subject's peripheral blood by the method of the invention is useful as a source of donor cells in bone marrow transplantation for the treatment of a variety of disorders, including cancer, anemia, autoimmunity and immunodeficiency. (justia.com)
The present invention relates generally to improved methods and pharmaceutical compositions for mobilizing hematopoietic stem and progenitor cell from bone marrow into peripheral blood by administration of at least one inhibitor of a GTPase, such as Rac1 and/or Rac2 GTPase. (justia.com)
Stem cells represent a very small percentage of bone marrow cells, and are normally quiescent. (justia.com)
These therapies decrease a patient's white blood cell counts, suppress bone marrow hematopoietic activity, and increase the patient's risk of infection and/or hemorrhage. (justia.com)
Current treatments to manage the problems that result from prolonged bone marrow suppression include the reinfusion of a patient's own previously harvested hematopoietic stem and progenitor cells. (justia.com)
In such procedures, patients undergo successive treatments with cell mobilization agents to cause mobilization of hematopoietic progenitor cells from the bone marrow to the peripheral circulation for harvesting. (justia.com)
After harvesting, the patient is given high dose chemotherapy or radiotherapy and the bone marrow function is reconstituted by infusion of the cells harvested earlier. (justia.com)
The use of high-dosage chemotherapy or radiotherapy for bone marrow ablation requires subsequent incorporation of hematopoietic stem cells into the patient, in which case prior harvesting of such cells is required. (justia.com)
The success of treatment crucially depends on the mobilization of the bone marrow stem cells, the subsequent return of which permits the patient to achieve reconstitution of a functioning hematopoietic system. (justia.com)
2004). Adundant expression of P2RY8 has been demonstrated in bone marrow CD34+ cells in leukaemic patients (Fujiwara et al. (atlasgeneticsoncology.org)

To educate its citizens about research into chimeras made from human and non-human animal cells, the United Kingdom's Human Fertilisation Embryology Authority published the consultation piece Hybrids and Chimeras: A Consultation on the Ethical and Social Implications of Creating Human/Animal Embryos in Research, in 2007. (asu.edu)
When two embryos are correctly joined before the 32-cell stage, the embryo will develop normally and exhibit a mosaic pattern of cells as an adult. (asu.edu)

Structure of the gene coding for the human early lymphocyte activation antigen CD69: a C-type lectin receptor evolutionarily related with the gene families of natural killer cell-specific receptors. (unige.ch)
There are eight mammalian P2Y receptors known to date ( P2Y1 , P2Y2 , P2Y4 , P2Y6 , P2Y11 , P2Y12 , P2Y13 and P2Y14 ) and they are found in most human tissues. (atlasgeneticsoncology.org)
Factors mandating cellular consequence include cell surface-receptors, cytoplasmic proteins, and nuclear proteins. (janechin.net)
The mammalian cell is furnished with receptors linked to interactive series of cytoplasmic networks for controlling cellular processes. (janechin.net)

Mammalian parental imprinting is a form of epigenetic regulation that causes genes to be expressed from only one chromosome homolog according to parent-of-origin 1 , 2 . (nature.com)
Trans -homolog interactions, such as homolog pairing, are highly structured and associated with gene function in Drosophila cells. (homologyeffects.org)

Robust allogeneic immune reactions after transplantation impede the translational pace of human embryonic stem cells (hESCs)-based therapies. (bvsalud.org)
Using haploid embryonic stem cells and gene editing, we fused the two largest mouse chromosomes, chromosomes 1 and 2, and two medium-size chromosomes, chromosomes 4 and 5. (bvsalud.org)
The remaining distal part of the IG-DMR was shown to bind pluripotency transcription factors in mouse embryonic stem cells (mESCs), exhibit active enhancer marks (H3K27ac), and nascent transcription 18 , 19 . (nature.com)

Here, we report programmed chromosome ligation in mice that resulted in the creation of new karyotypes in the lab. (bvsalud.org)
However, karyotypes carrying fused chromosomes 1 and 2 resulted in arrested mitosis, polyploidization, and embryonic lethality, whereas a smaller fused chromosome composed of chromosomes 4 and 5 was able to be passed on to homozygous offspring. (bvsalud.org)

DNA methylation and histone modifications play a central role in the epigenetic regulation of gene expression and cell differentiation. (cipsm.de)
This process of division and differentiation is subject to regulation at many levels to control cell production. (justia.com)
As CXXC1 is orthologous to Saccharomyces cerevisiae Spp1 that links DSB sites to the DSB machinery on the chromosome axis, we propose that these molecular interactions involved in the regulation of meiotic DSB formation are conserved in mouse meiosis. (springer.com)
Encouraging progress in understanding cell cycle regulation occurred over the past five years. (janechin.net)
Such regulation ensures faithful reproduction of DNA for subsequent distribution to daughter cells. (janechin.net)
2006. Regulation of B-cell tolerance by the lupus susceptibility gene Ly108. (uh.edu)

Mammalian parental imprinting represents an exquisite form of epigenetic control regulating the parent-specific monoallelic expression of genes in clusters. (nature.com)
A chimera instead contains discrete cell populations with two unique sets of parental genes. (asu.edu)

J Cell Biol (2007) 176 (1): 43-49. (rupress.org)
Nat Rev Mol Cell Biol. (ac.ir)

The frequency of cells containing 0 or 2 human chromosomes in the progeny of chemically-treated monochromosomal hybrid cells provided a direct measure of aneuploidy. (epa.gov)
Mutations in these cells (i.e., "generative" cells ancestral to the gametes) are transmitted to progeny while those in somatic cells are not. (lookformedical.com)

This is driven by cellular proteins which tether the lentiviral preintegration complexes to specific sites on the host chromosomes. (biomedcentral.com)
1993) suggested the production of a family of proteins by binucleate cells. (ucsd.edu)
Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. (lookformedical.com)

2005. Genetics of lupus susceptibility in NZB/SWR hybrids - lessons from an intercross study. (uh.edu)
Nothing is known about the transmission genetics of vertically transmitted Leishmania populations ( 8 , 20 , 21 ) or how the absence of vector stages affects the establishment or pathogenicity of mammalian infections. (cdc.gov)

Sequential rounds of division and differentiation give rise to an enormous amplification of cell numbers, necessary for the production of mature blood cells. (justia.com)
2007). However P2RY8 expression appears to be down-regulated during leucocyte differentiation: normal, mature peripheral blood leucocytes have been shown to have minimal expression, while an undifferentiated promyelocytic leukaemia cell line was shown to have moderate expression of P2RY8 that was down-regulated with induction of granulocyte differentiation (Adrian et al. (atlasgeneticsoncology.org)

An obvious requirement for the stringent control of cell cycle progression is the prevention of deregulated proliferation - loss of control may result in tumors and cancers. (janechin.net)
Chronic exposure of rats resulted in increased thyroid follicular cell tumors from sustained perturbation of thyroid hormone homeostasis. (cdc.gov)

Wooding (1982) reviewed the possible fusion of binucleate cells and endometrium, with granules moving to the maternal circulation. (ucsd.edu)

Any detectable and heritable alteration in the lineage of germ cells. (lookformedical.com)

82 Radiation hybrids are generated by using X-rays to randomly break chromosomes into fragments, then implanting the fragments into non-irradiated rodent cells, which replicate and thus clone the chromosomes. (wikipedia.org)
Furthermore, we observed that VSV-G-pseudotyped HIV-1 containing these IN mutants was unable to replicate in the C8166 T cell line and this defect was partially rescued by complementation with the catalytically inactive D64E IN mutant. (biomedcentral.com)

The formation of the DNA-RNA G-quadruplex causes a significant increase in the clonogenic capacity of cells and has an effect on inhibition of cellular senescence. (elsevierpure.com)
These effectors regulate the cytoskeleton, leading to a variety of cellular outputs including pronuclear migration, nuclear migration through constricted spaces, nuclear anchorage, centrosome attachment to nuclei, meiotic chromosome movements, and DNA damage repair. (ucdavis.edu)
The mammalian cell cycle typically completes in 24 hours, where dramatic changes occur in cellular metabolism and cytoskeletal physiology. (janechin.net)
Restriction points receive signals from central- and peripheral cellular networks to determine subsequent cell fate. (janechin.net)

A single human chromosome transferred into mouse cells was used as a cytogenetic marker to quantitate abnormal chromosome segregation following chemical treatment. (epa.gov)
The frequency of an abnormal segregation of the human chromosome was found to be dose dependent and consistently higher than controls. (epa.gov)
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. (yeastgenome.org)

Radiation hybrid mapping consists of several steps. (wikipedia.org)

On rare occasions, for example when very few F1 mice are obtained from the chimera, F1 heterozygous mice are crossed to 129/SvEvBrd x C57BL/6 hybrid mice to yield additional heterozygous animals for the intercross to generate the F2 mice. (mmrrc.org)

Radiation hybrid mapping (also known as RH mapping) is a technique for mapping mammalian chromosomes. (wikipedia.org)

These data, in conjunction with earlier human chromosome painting studies of the horse karyotype and synteny mapping of horse microsatellite markers physically mapped by FISH, confirm the assignment of UCD21 to ECA21 and suggest that UCD14 is located on ECA14. (etsu.edu)

Strikingly, flipping imprinting on the parental chromosomes by crossing genotypes of complete and partial intergenic element deletions rescues the lethality of each deletion on its own. (nature.com)
However, in earlier studies, clonal analysis was restricted by the lack of a cell marker, present at all times, that makes a distinction between the two parental cell types in situ. (asu.edu)

Imprints entail the maintenance of germline-derived differential epigenetic marks, mostly in the form of DNA methylation, through to the somatic cells of the offspring. (nature.com)

Crystal structure of human CD69: a C-type lectin-like activation marker of hematopoietic cells. (unige.ch)
Leukocytic, hematopoietic cells are important in maintaining the body's defense against disease. (justia.com)

The mammalian orthologs of Derailed and SRC64B also form complexes, suggesting that Src roles in RYK signaling are conserved. (biologists.com)
Each stage of the cell cycle is profiled by distinct protein complexes and phosphorylation events. (janechin.net)

Invited Perspective in The Journal of Cell Biology . (ucdavis.edu)

Preexisting antivector immunity can severely compromise the ability of Salmonella enterica serovar Typhimurium live vaccines to induce protective CD8 T-cell frequencies after type III secretion system-mediated heterologous protein translocation in orally immunized mice. (cipsm.de)

The Restriction point is also known as a checkpoint, where 'the cell is arrested at a particular phase of the cycle due to a lack of appropriate signals' (Hartwell and Weinert, 1989). (janechin.net)

T lymphocytes for adoptive cell therapy against hematological cancer. (upstate.edu)

Molecular characterization of the early activation antigen CD69: a type II membrane glycoprotein related to a family of natural killer cell activation antigens. (unige.ch)
Cell membrane. (atlasgeneticsoncology.org)
Membrane fusion drives pronuclear meeting in the one-cell embryo. (ucdavis.edu)

Dysregulation of processes driven by these factors is a severe liability to the cell and therefore represents potential areas for therapeutic intervention. (janechin.net)

7. Kim YG, Cha J, Chandrasegaran S. Hybrid restriction enzymes: Zinc finger fusions to Fok I cleavage domain. (ac.ir)

Comparative biochemical studies of rapamycin and wortmannin on transforming growth factor-beta 1 (TGF-beta1)-mediated G(1)-S cell cycle progression in C3H-10T1/2 embryonic fibroblasts. (janechin.net)
Therefore, understanding biochemical dynamics of cell cycle progression may lead to target-specific therapy with improved side effect profiles. (janechin.net)
These factors couple extracellular signals to intracellular processes to determine cell cycle progression. (janechin.net)

Use of a Human/Mouse Hybrid Cell Line to Detect Aneuploidy Induced by Environmental Chemicals. (epa.gov)
A short-term assay utilizing a human/mouse monochromosomal hybrid cell line R3-5, to detect chemically induced aneuploidy in mammalian cells is described. (epa.gov)
The human chromosome present in the mouse cells can be readily identified by differential staining procedures. (epa.gov)

The authors tested the sensitivity of the proposed system with three model chemicals (Colcemid, cyclophosphamide and benomyl) known to induce numerical or structural changes in chromosomes. (epa.gov)

In normal cells the BRCA1 protein is localized in the nucleus, whereas in the majority of breast cancer cell lines and in malignant pleural effusions from breast cancer patients, it is localized mainly in the cytoplasm. (lookformedical.com)

Furthermore, the protein may act to transmit signals in natural killer cells and platelets. (unige.ch)
Other cell types derived from hematopoietic stem cells include platelets and erythrocytes. (justia.com)

gem homozygous receptor mutant HCs display normal cell viability, afferent synaptogenesis, and peripheral innervation, yet exhibit strongly reduced extracellular potentials (∼50% of wild-type potentials). (jneurosci.org)

By epub Strategisches Controlling in international tätigen Konzernen: Aufgaben - Instrumente, joint cell grids make been creating the regulatory research Protein. (scoutconnection.com)
Using affinity purification-mass spectrometry and global phosphoproteomic and protein abundance analyses using three IAV strains (pH1N1, H3N2, H5N1) in three human cell types (A549, NHBE, THP-1), we map 332 IAV-human protein-protein interactions and identify 13 IAV-modulated kinases. (cdc.gov)

Compared with wide-type hESCs, HLA-A11R hESC-derived endothelial cells elicited much weaker immune responses to human HLA-A11+ T cells, while maintaining HLA-I molecule-mediated inhibitory signals to natural killer (NK) cells. (bvsalud.org)
Telomeric repeat-containing RNA, a non-coding RNA molecule, has recently been found in mammalian cells. (elsevierpure.com)
The detailed structural features and functions of the telomeric RNA at human chromosome ends remain unclear, although this RNA molecule may be a key component of the telomere machinery. (elsevierpure.com)

If two given DNA markers are far apart on the initial chromosome, then it is likely that they will appear in distinct fragments. (wikipedia.org)
Comparative anchor tagged sequences (CATS) from human Chromosome 5 (HSA5) were used as PCR primers to produce molecular markers for synteny mapping in the horse. (etsu.edu)

2001) suggested that apoptosis can be demonstrated in these cells. (ucsd.edu)

There has been much speculation on whether mammalian eggs with two male pronuclei can develop normally. (nature.com)

L-type Ca 2+ channels (LTCCs) drive the bulk of voltage-gated Ca 2+ entry in vertebrate inner ear hair cells (HCs) and are essential for mammalian auditory processing. (jneurosci.org)

Constraints on the design of primers were to avoid presence of long introns, whose position and length was predicted on the basis of the orthologous human gene structure, and to design primers in the most divergent regions of the human/chicken alignment, to limit cross-amplification with the hamster DNA present in the hybrids. (biomedcentral.com)
Likewise, numerous binucleate trophoblastic cells are present in the single-layered trophoblastic villous cover. (ucsd.edu)

4. Yokoyama S, Imoto K, Kawamura T, Higashida H, Iwabe N, Miyata T, and Numa S (1989) Potassium channels from NG108-15 neuroblastoma-glioma hybrid cells: primary structure and functional expression from cDNAs. (aspetjournals.org)
Fusion gene: a 320-kb large interstitial deletion within the pseudoautosomal region 1 (PAR1) on chromosome X [del(X)(p22.33p22.33)] and chromosome Y [del(Y)(p11.32p11.32)] results in juxtaposition of the first non-encoding exon of P2RY8 to the CRLF2 coding region to form P2RY8-CRLF2 fusion which leads to over-expression of full-length CRLF2. (atlasgeneticsoncology.org)

Since the Several mixed recombinase is a model Note, we can focus the longitudinal follow to deliver a Longitudinal example( P2) of Prior cell for later dose subjects. (scoutconnection.com)

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