• His studies have identified molecular mechanisms that underlie metabolic arrest across phylogeny and that support phenomena including mammalian hibernation, estivation, and anoxia- and ischemia-tolerance. (wikipedia.org)
  • Molecular studies include identification of genes that are up-regulated at different stages of the mammalian hibernation-arousal cycle and analysis of the actions of new proteins that induce metabolic depression or preserve life in the torpid state. (kenstoreylab.com)
  • Multi-tissue profile of NFKB pathway regulation during mammalian hibernation. (wijelab.ca)
  • We find that Kv2.1 and Kv2.2 exhibit distinct responses to acute phosphorylation-dependent regulation in brain neurons in situ and in heterologous cells in vitro . (jneurosci.org)
  • Metabolic reorganization in winter: Regulation of pyruvate dehydrogenase (PDH) during long-term freezing and anoxia. (wijelab.ca)
  • Dynamic regulation of six histone H3 lysine (K) methyltransferases in response to prolonged anoxia exposure in a freshwater turtle. (wijelab.ca)
  • Using Arabidopsis thaliana , we show guard cell GABA production is necessary and sufficient to reduce stomatal opening and transpirational water loss, which improves water use efficiency and drought tolerance, via negative regulation of a stomatal guard cell tonoplast-localised anion transporter. (nature.com)
  • Arnold PK, Finley LWS (2023) Regulation and function of the mammalian tricarboxylic acid cycle. (bioblast.at)
  • As illustrated in recent publications, the level of available oxygen in the cell significantly influences cell physiology, signal transduction, and cellular response to drug treatment. (bmglabtech.com)
  • 1.2.3 In spite of this knowledge, the majority of in vitro studies culture and study cells at ambient oxygen - ignoring the oxygen gradient between the atmosphere and the medium and between the medium and the intracellular cell environment. (bmglabtech.com)
  • To facilitate the quantification of cellular oxygenation Agilent Technologies has developed the MitoXpress Intra - Intracellular Oxygen Assay kit, based on a proprietary O 2 -sensitive cell-penetrating nanoparticle probe. (bmglabtech.com)
  • The MitoXpress Intra nanoparticle probe is taken up by cells during an overnight loading period and responds in real-time to any changes in intracellular oxygen concentration in both 2D cultures as well as a wide range of 3D systems, including Matrigel, RAFT™, microtissues, Alvetex ® , Mimetix ®, and other scaffolds. (bmglabtech.com)
  • In the specific example shown here (Fig. 1) for cells cultured under ambient oxygen, the intracellular [O 2 ] measured using MitoXpress Intra was found to be ~14% for metabolically inactive cells and ~7% for metabolically active cell types. (bmglabtech.com)
  • As the real oxygen concentration experienced by cells in culture is a function of environmental O 2 concentration, cell metabolism, and seeding density, MitoXpress Intra provides the ideal tool to intelligently modulate these parameters to achieve a desired and specific intra-cellular oxygen concentration. (bmglabtech.com)
  • 7 Severe hypoxia in the presence of energy stimulates cells to undergo apoptosis, whereas oxygen levels above 0.5% prevent cell death. (bmj.com)
  • This in turn renders the supply of oxygen in tumor vasculature insufficient, consequently leading to formation of hypoxia or anoxia regions in malignant tumors. (nature.com)
  • Cyanide prevents the cells of the body from using oxygen. (scienceoxygen.com)
  • 1) Gene expression studies identify genes that are up-regulated in response so hypoxia/anoxia and also evaluate the activity status of specific transcription factors and the suite of genes under their control in order to determine how anoxia tolerant systems respond when oxygen is withdrawn. (kenstoreylab.com)
  • We see that the area with hypoxia (low oxygen level) or anoxia (no oxygen) has spread to a large scale, the dead zone where there's no oxygen is extensive. (yahoo.com)
  • The oxygen sensing and signal transduction machinery is located in the pulmonary arterial smooth muscle cells (PASMCs) of the pre-capillary vessels, albeit the physiological response may be modulated in vivo by the endothelium. (ersjournals.com)
  • While the mammalian heart cannot function without oxygen, this is not true of all vertebrates. (silverchair.com)
  • Several vertebrate species can survive anoxia, the complete absence of oxygen, for days or even months. (silverchair.com)
  • It would be of interest to investigate in more detail the biochemical and molecular bases of continued cardiac function during anoxia. (silverchair.com)
  • Expression of nucleoside diphosphate kinase, mitochondria-associated adenylate kinase, and several mitochondria-associated creatine kinase isozymes was highest in the outer retina, whereas expression of cytosolic adenylate kinase and brain creatine kinase was higher in the cones, horizontal cells, and amacrine cells indicating the diversity of ATP-buffering strategies among retinal neurons. (molvis.org)
  • The Kv2 family of voltage-gated potassium channel α subunits, comprising Kv2.1 and Kv2.2, mediate the bulk of the neuronal delayed rectifier K + current in many mammalian central neurons. (jneurosci.org)
  • Analyses of endogenous Kv2.2 in cortical neurons in situ and recombinant Kv2.2 expressed in heterologous cells reveal that Kv2.2 is largely refractory to stimuli that trigger robust, phosphorylation-dependent changes in Kv2.1 clustering and function. (jneurosci.org)
  • SIGNIFICANCE STATEMENT Neurons within the neocortex are arranged in a laminar architecture and contribute to the input, processing, and/or output of sensory and motor signals in a cell- and layer-specific manner. (jneurosci.org)
  • Sensorineural (nerve) deafness is loss of auditory function because of loss of cochlear hair cells or auditory nerve neurons. (vin.com)
  • In in vitro tests, riluzole protected cultured rat motor neurons from the excitotoxic effects of glutamic acid and prevented the death of cortical neurons induced by anoxia. (nih.gov)
  • The ACU is a microprocessor-controlled unit that can regulate CO 2 and O 2 within the reader to reproduce the optimal physiological as well as hypoxic conditions needed for live cell-based assays . (bmglabtech.com)
  • However, the effect of H2S on HIF-1 activity under hypoxic conditions is largely unknown in mammalian cells. (starrlifesciences.com)
  • Hearing loss is the most common sensory deficit in humans, and may also be so in companion animals where it affects all mammalian species. (vin.com)
  • Our study provides a proof of principle that unique longevity mechanisms that evolved in long-lived mammalian species can be exported to improve the lifespans of other mammals," study co-author Vera Gorbunova said. (yahoo.com)
  • Applied studies use the lessons taken from freeze tolerant vertebrates to improve the cryopreservation of isolated mammalian cells and organs. (kenstoreylab.com)
  • The Characterization of humanin-like mitochondrial peptide in anoxia tolerant freshwater turtle. (wijelab.ca)
  • These anoxia-tolerant animals include freshwater turtles in the genera Chrysemys and Trachemys , and the crucian carp Carassius carassius . (silverchair.com)
  • Does the onset of ocean anoxia at the end of many of these events suggest a possibility of cycle where the collapse of one civilization provides the seeds (fossil fuels) for the next? (realclimate.org)
  • A substantial portion of free or bound creatine and creatine phosphate are found in mammalian skeletal muscle tissue. (oatext.com)
  • In the present study, we describe a process for the production of recombinant phenotype specific Hp polymers in mammalian FS293F cells. (biomedcentral.com)
  • Here, through combined use of cortical layer markers and transgenic mouse lines, we show that Kv2.1 and Kv2.2 are localized to functionally distinct cortical cell types. (jneurosci.org)
  • Here, we show that the Kv2 family members Kv2.1 and Kv2.2 are expressed in distinct cortical layers and pyramidal cell types associated with specific corticostriatal pathways. (jneurosci.org)
  • Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E (2022) Physiological cell culture media tune mitochondrial bioenergetics and drug sensitivity in cancer cell models. (bioblast.at)
  • Ganguly U, Bir A, Chakrabarti S (2022) Cytotoxicity of mitochondrial Complex I inhibitor rotenone: a complex interplay of cell death pathways. (mitophysiology.org)
  • In vitro endothelial cell adhesion experiments demonstrate that, as creatine concentrations increase, endothelial cell surface adhesion and permeability were both modified. (oatext.com)
  • Innovation: For the first time, we show that H2S modulates intracellular O2 homeostasis and regulates activation of HIF-1 and the subsequent gene expression induced by hypoxia by using an in vitro system with established cell lines and an in vivo system in mice. (starrlifesciences.com)
  • Storey's studies of the adaptations that allow frogs, insects, and other animals to survive freezing have made major advances in the understanding of how cells, tissues and organs can endure freezing. (wikipedia.org)
  • The severity of hypoxia determines whether cells become apoptotic or adapt to hypoxia and survive. (bmj.com)
  • Mammalian cells have developed a range of adaptations to survive acute and even prolonged hypoxia. (bmj.com)
  • Vercellino I, Sazanov LA (2021) Structure and assembly of the mammalian mitochondrial supercomplex CIII2CIV. (mitophysiology.org)
  • Results: The H2S donors sodium hydrosulfide and sodium sulfide in pharmacological concentrations reversibly reduced cellular O2 consumption and inhibited hypoxia- but not anoxia-induced HIF-1α protein accumulation and expression of genes downstream of HIF-1 in established cell lines. (starrlifesciences.com)
  • New insights into cell-specific and compartmental ATP and GTP production, as well as utilization and buffering strategies and their relationship with known retinal and cellular functions, are discussed. (molvis.org)
  • This prevents the electron transport chain (the last part of cellular respiration) from working, meaning that the cell can no longer produce ATP for energy. (scienceoxygen.com)
  • Multiple research groups have developed artificial mitochondrial transfer/transplantation (AMT/T) methods that transfer healthy mitochondria into damaged cells and recover cellular function. (biomedcentral.com)
  • Cells will adapt by activating the expression of genes involved in metabolic adaptation, such as those involved in glycolysis. (bmj.com)
  • 3- 5 Therefore, during severe hypoxia or anoxia, the cell initiates a cascade of events that leads to apoptotic cell death, thereby preventing the accumulation of cells with hypoxia induced mutations. (bmj.com)
  • Experimental evidence adopting von Hippel-Lindau (VHL)- or mitochondria-deficient cells indicated that H2S did not affect neosynthesis of HIF-1α protein but destabilized HIF-1α in a VHL- and mitochondria-dependent manner. (starrlifesciences.com)
  • The cyanide ion, CN, binds to the iron atom in cytochrome C oxidase in the mitochondria of the cells and acts as an irreversible enzyme inhibitor. (scienceoxygen.com)
  • Mitochondria are essential organelles that not only serve as energy factories, but also have functions extending to cell signalling. (biomedcentral.com)
  • Based on these findings, the researchers proposed the bold idea of directly transplanting entire mitochondria, rather than drugs or whole cells, into lesions to explore the rescue effects of exogenous mitochondria. (biomedcentral.com)
  • 27. T ljedal I-B: Presence, inducation and possible role of glucose 6-phosphatase in mammalian pancreatic islets. (taljedal.se)
  • 29. T ljedal I-B: Glucose-6-phosphatase in pancreatic -cell metabolism. (taljedal.se)
  • It is naturally produced in bacteria and displays a pore-forming activity that lyses mammalian erythrocytes 16 . (nature.com)
  • However, when the environmental O 2 concentration is reduced to ~6% using an ACU, the intracellular [O 2 ] falls to ~4.5% for metabolically less active cells and is close to anoxia for metabolically active cells. (bmglabtech.com)
  • Time-lapse videography of living cells captures spectacular intracellular mitochondrial movement, and this movement promotes mitochondrial connections to form a dynamic mitochondrial network [ 3 ]. (biomedcentral.com)
  • In addition to intracellular mitochondrial movement, intercellular mitochondrial transfer between mammalian cells has recently been discovered. (biomedcentral.com)
  • Intercellular mitochondrial transfer can be regarded as an extension of intracellular mitochondrial movement or intercellular communication, which undoubtedly increases the mtDNA content of the recipient cells and restores the respiration and survival of the recipient cells. (biomedcentral.com)
  • Cells adapt to this environmental stress, so that after repeated periods of hypoxia, selection for resistance to hypoxia induced apoptosis occurs. (bmj.com)
  • In this study, we tried to elucidate the effect of H2S on hypoxia-induced HIF-1 activation adopting cultured cells and mice. (starrlifesciences.com)
  • Our goals were twofold: 1) to characterize the gene expression, protein expression, and activity of key synthesizing and regulating enzymes of energy metabolism in the whole mouse retina, retinal compartments, and/or cells and 2) to provide an integrative analysis of the results related to function. (molvis.org)
  • Biochemical approaches include studies of stress-activated protein kinase cascades and reversible protein phosphorylation control of the activities of metabolic enzymes and functional proteins to coordinate global metabolic suppression and ensure long term cell survival over weeks of torpor. (kenstoreylab.com)
  • Mitogen-activated protein kinases and the cell stress response. (wijelab.ca)
  • Preclinical studies have evaluated haptoglobin (Hp) polymers from pooled human plasma as a therapeutic protein to attenuate toxic effects of cell-free hemoglobin (Hb). (biomedcentral.com)
  • The toxicity of cyanide is linked mainly to the cessation of aerobic cell metabolism. (scienceoxygen.com)
  • Creatine supplementation, in and of itself, has been shown to influence endothelial permeability and cell surface reactivity to a modest degree, potentially interfering/blocking an inflammatory stimulus. (oatext.com)
  • The effects of macrophage NLRP3 inflammasome activation on the function of hepatic stellate cells (HSCs) were explored by treating primary HSCs with preconditioned media from BMDMs culture. (bvsalud.org)
  • To find out, Nilsson and his team examined cardiac function in the crucian carp over a period of 5 days of anoxia at 8±1°C. In a paper recently published in Science , they report that following an initial 24 h adjustment period of increased stroke volume and cardiac output, the carp's cardiac function returned to normal levels. (silverchair.com)
  • 9. Hellerstr m C, Hellman B & T ljedal I-B: Quantitative studies on phosphatases in the pancreatic islet -cells. (taljedal.se)
  • As an alternative to the rapidly dying mammalian models, this could reveal new treatments in the face of mammalian heart failure. (silverchair.com)
  • HO-1 in Cancer Cell Survival" Encyclopedia , https://encyclopedia.pub/entry/14608 (accessed December 06, 2023). (encyclopedia.pub)
  • The alkalinity of soda has shown anti-proliferative effects on tumor cell lines. (oatext.com)
  • 1. Equipped with HlyE, EcNe significantly caused tumor regression in mice xenografted with human colorectal cancer cells. (nature.com)
  • Nevertheless, they have intrinsic limitations that most of the produced payloads are restricted to proliferating cells or/and afflicted with tumor penetration. (nature.com)
  • Research focuses on the biochemistry of metabolic arrest, in particular the mechanisms that regulate and coordinate the depression of all cell functions in concert to permit long term homeostasis in the torpid state. (kenstoreylab.com)
  • Based on the antibody intensities and the COX and LDH activity, Müller glial cells (MGCs) had the lowest capacity for glycolysis, aerobic glycolysis, and OXPHOS. (molvis.org)
  • Modulation of mitochondrial electron transport chain activity differentially regulates cell death in proliferating and quiescent cells. (mitoeagle.org)
  • Previous studies have shown that compared to mice and humans, the mole rats have about 10 times more HMW-HA in their bodies, and when the molecule's gene was removed from the rats, their cells were more likely to form tumours. (yahoo.com)
  • In addition, cell proliferation and angiogenesis will be stimulated, enabling better oxygenation of the tissue. (bmj.com)
  • Proof of concept studies have demonstrated efficacy of Hp in hemolysis associated with transfusion and sickle cell anemia. (biomedcentral.com)
  • Structural and functional studies of Cytochrome P450 in mammalian cells. (metu.edu.tr)
  • Furthermore, FZHY was observed to block the activation of the NLRP3 inflammasome pathway, which can lead to excessive inflammatory cytokine release in supernatants and cell lysates in response to LPS and ATP. (bvsalud.org)
  • Recent reports have shown that an artificially created alkaline environment can influence cell membrane permeability and cell behavior. (oatext.com)
  • The ability of alkaline buffered creatine to produce micro-environmental remodeling, influence cell membrane behavior and impact cisplatin-mediated toxicity, are also examined in this paper. (oatext.com)
  • A hypoxic environment devoid of nutrients prevents the cell undergoing energy dependent apoptosis and cells become necrotic. (bmj.com)
  • Low ATP concentrations in hypoxic tumour cells disable the apoptotic cascade and induce cell death by necrosis. (bmj.com)
  • The restricted pattern of Kv2.2 expression persists in Kv2.1-KO mice, suggesting distinct cell- and layer-specific functions for these two highly related Kv2 subunits. (jneurosci.org)
  • HOs are expressed in a variety of cell types, rendering their broad contribution to cell functions. (encyclopedia.pub)
  • In fact, the only cardiovascular functions that seemed to have changed over 2-5 days of anoxia were the fish's peripheral vascular resistance and blood pressure in the ventral aorta, which continued to be depressed, indicating that the fish's blood vessels had dilated. (silverchair.com)
  • Roughly 95% of creatine is released in close proximity to the muscle tissue and transported into the cell via a limited capacity creatine-transporter system. (oatext.com)
  • Creatine is pivotally important in delaying ATP depletion during anoxia or ischemia through the creatine-phosphocreatine system [ 10 ,11]. (oatext.com)
  • That gives us a way to study the mechanism that kills the bacterial cells. (sflorg.com)