• HA is a high molecular weight polysaccharide found in virtually all mammalian tissues and body fluids, where it fills the space between cells and fibers and acts as a lubrificant as well as a barrier to penetration by foreign particles. (unesp.br)
  • Ectoderm is one of three germ layers--groups of cells that coalesce early during the embryonic life of all animals except maybe sponges, and from which organs and tissues form. (asu.edu)
  • Because the expression of human and mouse Survivin splice variants in cancer cells continues to be proven on the mRNA level, we performed RT PCR to examine the presence of SurvivinGp splice kinds in adult guinea pig tissues. (mirnamimic.com)
  • A single-cell layer of epithelium is separated from the tissues beneath it by a supporting substance called the extracellular matrix. (elifesciences.org)
  • Understanding how cancerous cells gain this ability may lead to new approaches to stopping tumor cells from dividing and colonizing tissues around the body. (elifesciences.org)
  • This behavior is particularly evident in the cells that divide aggressively to form tumors that subsequently migrate and colonize other tissues around the body. (elifesciences.org)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • Oncogenic mutations or abnormal expression of signaling components disrupt the regulatory networks that govern cell function, thus enabling tumor cells to undergo dysregulated mitogenesis, to resist apoptosis, and to promote invasion to neighboring tissues. (eurekaselect.com)
  • Two cannabinoid receptors have been characterized and cloned from mammalian tissues: the "central" CB 1 receptor and the "peripheral" CB 2 receptor. (aacrjournals.org)
  • These properties include lack of pathogenicity and pathology, ease of preparation and purification, long term expression in many tissues including the muscle, and lack of a detrimental cell-mediated immune response. (justia.com)
  • The chimeric virus can also have a reduced transduction capability with respect to one or more cells or tissues (e.g., liver), which can be desirable in terms of targeting the vector to the target tissue of interest and reducing dosage of vector to be administered. (justia.com)
  • Systems toxicology, therefore, aims at understanding and exploring the way that different biological components are orchestrated as an ensemble in cells, tissues, and organisms. (brill.com)
  • The tumor suppressor gene overgrown hematopoietic organs-31 (oho31) of Drosophila encodes a protein with extensive homology to the Importin protein of Xenopus (50% identity), the related yeast SRP1 protein, and the mammalian hSRP1 and RCH1 proteins. (rupress.org)
  • We have cloned the oho31 gene of Drosophila melanogaster and determined its nucleotide sequence. (rupress.org)
  • The synthesized gene was subsequently cloned into an expression vector. (cusabio.com)
  • Amazingly, the first human cancer gene was cloned only thirty years ago. (jcancer.org)
  • In summary, our data highlight MCT4 /SLC16A3 as a key gene for distinct hallmarks of tumor malignancy in glioma cells. (hindawi.com)
  • Human BeWo cell line has been used to study the effects of Δ-9-tetrahydrocannabinol (Δ9-THC) on the proliferation and gene transcription in the BeWo trophoblast cell line. (sigmaaldrich.com)
  • This approach uses a modified piggyBac transposon to generate libraries of mutagenized cells, each containing transposon insertions that randomly activate nearby gene expression. (biomedcentral.com)
  • Using this approach we successfully identify genes involved in paclitaxel resistance in a variety of cancer cell lines, including the multidrug transporter ABCB1, a previously identified major paclitaxel resistance gene. (biomedcentral.com)
  • In an try to validate experimentally the three in silico identified BCLL splice variants , we also found and cloned numerous alternatively spliced variants within the BCLL gene , almost all of which showed a tissue specified pattern of expression. (mirnamimic.com)
  • CD19 antigen is present on human B lymphocytes at most sTages of maturation, from the earliest Ig gene rearrangement in pro-B cells to mature cell, as well as malignant B cells, but is lost on maturation to plasma cells. (bio2009.org)
  • They found that the gene that codes for a protein called poliovirus receptor-like 4 (PVRL4) allows attachment-free cell growth and also makes cells cluster together once detached from the matrix. (elifesciences.org)
  • Normally, the PVRL4 gene is not active in breast epithelial cells, but its activity is detected in many breast, lung, and ovarian tumors. (elifesciences.org)
  • hIFNγ was cloned into the pPIC9 vector adjacent to the HIS4 gene, a gene essential for histidine biosynthesis, which was then transformed into P. pastoris. (edu.au)
  • The resultant DNA is placed in a mammalian cell culture, which then expresses the resultant gene, producing the desired antibody. (msdmanuals.com)
  • Most of these drugs target BTK or PI3K, although inhibitors of SYK, SRC family kinases and mammalian target of rapamycin (mTOR) have also demonstrated activity in clinical trials [11]. (unicatt.it)
  • Although the optimal treatment strategy continues to evolve, three agents that target angiogenesis (sunitinib, bevacizumab, and pazopanib) and a mammalian target of rapamycin (mTOR)-targeted therapy (temsirolimus) have been approved as front-line agents. (medscape.com)
  • In the 1940s she became the first person to successfully clone a mammalian cell in vitro, which allowed for more efficient means of creating pure strains of cells for study. (wikipedia.org)
  • It did not take her long to make an impact, as almost immediately after joining she made her largest discovery involving a method of cloning mammalian cancer cells in vitro. (wikipedia.org)
  • Working with Dr. Virginia Evans and a group of tissue-culture workers she became the first to successfully clone a mammalian cell in vitro. (wikipedia.org)
  • Her research targeted isolated mammalian cells in vitro and finding ways to trigger malignant transformations and replication. (wikipedia.org)
  • Distinct hallmarks of cancer were examined using in silico analysis, various in vitro cell culture assays, and ex vivo organotypic rat brain slice culture model. (hindawi.com)
  • We investigated the impact on tumor malignancy in F98 glioma cells using in silico analysis, in vitro cell culture assays, and ex vivo vascular organotypic glioma impact model (VOGIM) [ 35 ] by implanting F98 cells into rat brain slices. (hindawi.com)
  • We have developed a powerful pipeline to systematically discover drug resistance in mammalian cells in vitro . (biomedcentral.com)
  • Our in vitro, ex vivo and in vivo results demonstrated that such antibody was able to promote depletion of the malignant cells by antibody-dependent cell-cytotoxicity or -phagocytosis. (hipi-lab-saint-louis.fr)
  • Cells grown in vitro from neoplastic tissue. (lookformedical.com)
  • With the recent advances in human-cell cultivation techniques, allowing in vivo -like in vitro long-term functionality, there is a shift in focus towards the mechanistic details of the adverse effects "over time" aimed at a better understanding of the dynamics of biological processes. (brill.com)
  • In vitro methods, based on human primary cells, cell lines, and genetically modified reporter cell lines, have greatly expanded the scope of in vitro toxicology. (brill.com)
  • Many targets have been identified in solid tumors for antibody therapy but it is less clear what surface antigens may be most commonly expressed on disseminated tumor cells. (bibf1120.com)
  • Monocarboxylate transporter 4 (MCT4, SLC16A3 ) is elevated under hypoxic conditions in many malignant tumors including gliomas. (hindawi.com)
  • Malignant gliomas are the most common primary malignant brain tumors with an increasing incidence of up to nine per 100,000 habitants over the last years [ 1 , 2 ]. (hindawi.com)
  • Effective immunotherapy of stromal-rich tumors requires simultaneous targeting of cancer cells and immunosuppressive elements of the microenvironment. (aacrjournals.org)
  • Furthermore, growth of orthotopically implanted tumors in vivo is inhibited by blocking PVRL4-driven cell-to-cell attachment with monoclonal antibodies, demonstrating a novel strategy for targeted therapy of cancer. (elifesciences.org)
  • It is estimated that up to 90% of cancers in humans originate in epithelial tissue, and the cells within such tumors are known to survive and divide even when they are no longer attached to the extracellular matrix. (elifesciences.org)
  • used genetic techniques to silence PVRL4 in cells from breast tumors, they found that it reduced the formation of clusters by the cancer cells and also reduced their ability to grow in the absence of attachment. (elifesciences.org)
  • Increasingly, renal cell cancers are diagnosed at an earlier stage, and nephron-sparing surgery and thermal ablation are gaining acceptance as a treatment of choice for smaller tumors. (medscape.com)
  • These ATG genes are highly conserved in mammalian cells, allowing to study their functions also in higher eukaryotes [ 1 - 4 ]. (hindawi.com)
  • The nontargeted control uses an shRNA sequence targeting no known mammalian genes (Sigma-Aldrich SHC002V). (bibf1120.com)
  • Most of the affected genes cluster into one of several distinct biological pathways, including cell cycle, DNA damage response, RNA metabolism, and BCR, Toll-like receptor (TLR), Notch and NF-kB signaling. (unicatt.it)
  • Analysis of co-occurring transposons integration sites in single cell clone allows for the identification of genes that might act cooperatively to produce drug resistance a level of information not accessible using RNAi or ORF expression screening approaches. (biomedcentral.com)
  • DEAF-1 activates the expression of Mtk and Drs promoter-luciferase fusion genes in S2 cells. (sdbonline.org)
  • A gain of function screen for genes that enable proliferation independently of matrix anchorage identified a cell adhesion molecule PVRL4 (poliovirus-receptor-like 4), also known as Nectin-4. (elifesciences.org)
  • explored which genes enable epithelial cells from the human mammary gland to grow without being attached to the extracellular matrix. (elifesciences.org)
  • Genetic studies of the families at high risk for developing renal cancer led to the cloning of genes whose alteration results in tumor formation. (medscape.com)
  • Due to its unique biophysical properties, HA contributes directly to tissue homeostasis, interacts with link proteins and proteoglycans (PGs) thus maintaining the structural integrity of extracellular and pericellular matrices, and its interaction with cell surface HA receptors mediates crucial influences of HA on cell behavior. (biomedcentral.com)
  • We will also look at the combination of mAbs with immunostimulants such as GMCSF and IL-2 (fusion proteins) and eventual conjugation of the mAbs with alpha and possibly B -emitters to help in targeting bystander cells. (jcancer.org)
  • The chemical changes mediated by reactive species RS are detrimental to cell function because they cause oxidation and nitration, altering the structures of cellular proteins, DNA, and lipids, and impairing their normal function. (encyclopedia.pub)
  • Emflex) integrity via DNA damage repair then becomes essential in both germ-line and somatic cells [2 5 6 Poly(ADP-ribosyl)ation is a post-translational modification mediated by poly(ADP-ribose) polymerase (PARP) enzymes in which negatively charged ADP-ribose units are transferred from donor nicotinamide adenine dinucleotide (NAD+) molecules onto target proteins [7]. (bio2009.org)
  • Killing Glioma 'Stem-like' Cells via Drug-Induced Relocation of Endosomal Urokinase Proteins. (ucdavis.edu)
  • Mis-trafficking of endosomal urokinase proteins triggers drug-induced glioma nonapoptotic cell death. (ucdavis.edu)
  • The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. (lookformedical.com)
  • The PGS candidate should have experience in mammalian cell cultivation techniques, molecular cloning and basic fluorescence microscopy. (stars-natur.cz)
  • A strong reduction in the expression of oho31 by a P element inserted in the 5' untranslated region of the oho31 transcript or a complete inactivation of oho31 by imprecise P element excision leads to malignant development of the hematopoietic organs and the genital disc, as shown by their growth autonomy in transplantation assays. (rupress.org)
  • The interaction of PKN3 and its effect on these newly discovered substrates and interactors will be evaluated by biochemical analyses and subsequent cell-based assays in cancer cell line and endothelial cell models. (stars-natur.cz)
  • We describe here the molecular cloning of IC1. (embl.de)
  • Despite the fact that Survivin is beneath intense investigation in human medicine, comparatively little is acknowledged relating to its expression and molecular perform in mammalian animal designs except mouse. (mirnamimic.com)
  • the development of strategies by tumor cells to adapt and escape to antibody or protein inhibitors-based therapies. (hipi-lab-saint-louis.fr)
  • Our main goal is to identify new tumor antigens specifically expressed at the tumor cell surface and to validate their potential use as new therapeutic targets through the development of antibody-based immune-therapies. (hipi-lab-saint-louis.fr)
  • Murine monoclonal antibodies are produced by injecting a mouse with an antigen, harvesting its spleen to obtain B cells that are producing antibody specific to that antigen, fusing those cells with immortal mouse myeloma cells, growing these hybridoma cells (eg, in cell culture), and harvesting the antibody. (msdmanuals.com)
  • In both cases, the process usually begins as above with production of mouse hybridoma cells that make antibody to the desired antigen. (msdmanuals.com)
  • Our team has expertise in skin cancers, with a special interest in cutaneous T cell lymphoma, melanoma and carcinoma. (hipi-lab-saint-louis.fr)
  • Our team develops research projects dedicated to skin cancers with a main interest on melanoma, cutaneous T cell lymphoma (CTCL) and carcinoma. (hipi-lab-saint-louis.fr)
  • 1982. Malignant melanoma and drinking water contamination. (cdc.gov)
  • While the importance of autophagy for cell homeostasis and survival has long been appreciated, its role in tumorigenesis and cancer progression is still developing [ 10 , 11 ]. (hindawi.com)
  • In tumor cells, PKN3 has been shown to act downstream of PI3-kinase activation to promote malignant progression of prostate cancer. (stars-natur.cz)
  • However, these defects are frequently found in only a small fraction of the malignant clones at the time of progression, suggesting that other mechanisms are also involved [15]. (unicatt.it)
  • During all stages of tumor progression, cancer cells are subjected to inappropriate extracellular matrix environments and must undergo adaptive changes in order to evade growth constraints associated with the loss of matrix attachment. (elifesciences.org)
  • Telomere shortening is involved in the progression of CELL AGING. (lookformedical.com)
  • We noticed three specific populations in malignant pleural effusions: ((malignant EpCAM+ EpCAM? (bibf1120.com)
  • Basically, given the difference of telomere and telomerase activity in human and mouse cells, the telomere and telomerase status in stem cell populations is different between humans and mice ( Harrington, 2004 ). (nature.com)
  • Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)
  • We used primary acute myeloid leukemia (AML) samples and human AML cell lines to investigate the regulatory mechanisms of autophagy and its role in AML differentiation. (hindawi.com)
  • Cellular differentiation of this stem cell type occurs when hormonal precursors are added to the medium. (sigmaaldrich.com)
  • Some of these agents target the embryonic period's "window of susceptibility," three to eight weeks after a pregnant woman's last menstruation, when the highest degree of sensitivity to embryonic cell differentiation and organ formation occurs. (asu.edu)
  • Somatic cells are cells that have gone through the differentiation process and are not germ cells. (asu.edu)
  • Expression is particularly high in the pancreas where it is thought to play a role in the differentiation of pancreatic beta cells. (embl.de)
  • On the surface of lymphoid cells, myeloid cells and other non-hematopoietic tissue cells, the expression of CD38 shows is lower, but on the surface of malignant plasma cells such as myeloma cells, CD38 shows high expression. (cusabio.com)
  • These cells provide proliferation and survival signals to the leukemic B cells by secreting different chemokines and cytokines or by expressing distinct surface receptors or adhesion molecules. (unicatt.it)
  • Cells involved in innate immunity recognize foreign substances such as bacteria with toll-like receptors (TLR) and regulate the activation of other cells by the production of various cytokines [ 4 ] . (encyclopedia.pub)
  • γ-Aminobutyric acid A (GABA A ) receptors (GABARs) are responsible for most fast inhibitory neurotransmission in the mammalian brain. (aspetjournals.org)
  • We measured its effects on recombinant GABARs to determine its mechanism of action at mammalian receptors and to examine the effect of subunit composition. (aspetjournals.org)
  • In particular, emerging evidence suggests that agonists of cannabinoid receptors expressed by tumor cells may offer a novel strategy to treat cancer. (aacrjournals.org)
  • Although anticancer effects of cannabinoids were shown as early as 1975 in Lewis lung carcinoma (ref. 1 and references therein), renewed interest was generated little after the discovery of the cannabinoid system and cloning of the specific cannabinoid receptors ( 1 ). (aacrjournals.org)
  • Although these diseases arise from the transformation of different cell types (namely melanocytes, CD4+ T cell and epithelial cells), they share common features such as a partially elucidated pathophysiology, a lack of treatment at advanced stages, a need to understand the mechanisms leading to relapse or resistance upon currently available treatments and consequently a need for alternative therapies. (hipi-lab-saint-louis.fr)
  • Notably, the pathophysiology of these diseases is still controversial especially regarding the tumor cell origin or the tumorigenic triggering factors. (hipi-lab-saint-louis.fr)
  • Consequently we utilized lineage markers-CD45 for hematopoietic cells and EpCAM for epithelial cells-to provisionally distinct the pleural effusions into different subsets. (bibf1120.com)
  • This mechanism preserves the single-cell layer pattern throughout the body and prevents epithelial cells from growing in inappropriate locations. (elifesciences.org)
  • The aim of this project will be to analyze the crosstalk of PKN3 and Rho signaling in cancer cells, focusing on their potential synergistic effect on F-actin dynamics and tumor cell motility in both 2D and 3D environments. (stars-natur.cz)
  • If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. (lookformedical.com)
  • We discuss the rationale of various anti-fibrogenic treatment strategies targeting the clonal hematopoietic stem/progenitor cell, aberrant signaling pathways, fibrogenic cytokines, and the tumor microenvironment. (haematologica.org)
  • A critical length of telomere repeats is required to ensure proper telomere function and avoid the activation of DNA damage pathways that result in replicative senescence or cell death. (nature.com)
  • Distinct pathways of cell migration and antiapoptotic response to epithelial injury: structure-function analysis of human intestinal trefoil factor. (embl.de)
  • Cancer cell biology takes advantage of identifying diverse cellular signaling pathways that are disrupted in cancer. (eurekaselect.com)
  • Signaling pathways are an important means of communication from the exterior of cell to intracellular mediators, as well as intracellular interactions that govern diverse cellular processes. (eurekaselect.com)
  • Cripto-1 overexpression in U87 glioblastoma cells activates MAPK, focal adhesion and ErbB pathways. (eurekaselect.com)
  • As the percentage of stromal cells could be >90% of the full total population acquiring the global ordinary across different cell types would skew the effect toward the greater abundant cell types and preclude antigen finding in uncommon tumor-forming cells. (bibf1120.com)
  • TheextracellularsignalsthatdriveCLLincludeinteractionswithvariouscellularelementspresent in the lymph node tumor microenvironment, such as T cells, monocyte-derived nurse-like cells and stromal cells [8]. (unicatt.it)
  • Distribution of CD44 variant isoforms in human skin: differential expression in components of benign and malignant epithelia. (geneticsmr.com)
  • Owing to the genomic instability present in this disease, patients with Bloom syndrome show a much higher incidence of malignant neoplasms, which is the major cause of death in such patients. (medscape.com)
  • Renal cell carcinoma (RCC) accounts for approximately 3% of adult malignancies and 90-95% of neoplasms arising from the kidney. (medscape.com)
  • In the second half of the twentieth century, scientists learned how to clone organisms in some species of mammals. (asu.edu)
  • In contrast to other PKN kinases, PKN3 is physiologically expressed mainly in primary endothelial cells and osteoclasts but is also frequently overexpressed in tumor cells. (stars-natur.cz)
  • In somatic cells, the activity of telomerase, a reverse transcriptase that can elongate telomeric repeats, is usually diminished after birth so that the telomere length is gradually shortened with cell divisions, and triggers cellular senescence. (nature.com)
  • Thus, even in stem cells, except for embryonal stem cells and cancer stem cells, telomere shortening occurs during replicative ageing, possibly at a slower rate than that in normal somatic cells. (nature.com)
  • Telomeric DNA consists of short guanine-rich repeat sequences in all eukaryotes with linear chromosomes, and its length in human somatic cells is remarkably heterogeneous among individuals ranging from 5 to 20 kb, according to age, organ, and the proliferative history of each cell ( Wright and Shay, 2005 ). (nature.com)
  • In most human somatic cells except for stem cells and lymphocytes, telomerase activity is diminished after birth so that telomere length shortens with each cell division. (nature.com)
  • Scientists have applied somatic cell nuclear transfer to clone human and mammalian embryos as a means to produce stem cells for laboratory and medical use. (asu.edu)
  • Somatic cell nuclear transfer (SCNT) is a technology applied in cloning, stem cell research and regenerative medicine. (asu.edu)
  • BML mutations thus result in defects in DNA repair and genomic instability in the somatic cells, predisposing the patients to cancer development. (medscape.com)
  • Mycosis Fungoides and Sézary syndrome are two forms of cutaneous T cell lymphoma associated to a poor prognosis at advanced stages due to a lack of curative treatments. (hipi-lab-saint-louis.fr)
  • Cutaneous T cell lymphoma (CTCL) is a heterogeneous group of non-Hodgkin lymphomas that originate from the skin. (hipi-lab-saint-louis.fr)
  • Our previous work allowed the identification of the first positive marker for MF and SS cutaneous and circulating malignant T cells, namely the NK cell receptor KIR3DL2. (hipi-lab-saint-louis.fr)
  • The overall result of the genomic instability in the proliferating cells is a high risk of malignancy, reduced fertility or infertility, B- and T-cell immunodeficiencies, and cutaneous manifestations, including photosensitivity, poikiloderma, and telangiectatic erythema. (medscape.com)
  • Additionally, she was a member of the American Society for Cell Biology, the American Society of Human Genetics, the Tissue Culture Association, and the International Society for Cell Biology. (wikipedia.org)
  • Prior to her discovery, tissue cultures had to be composed of multiple and various human body cells in order for them to survive and grow. (wikipedia.org)
  • Hyaluronic acid levels are markedly increased during the embryogenesis, inflammation, malignant transformation and whenever fast tissue turnover and remodeling is required. (unesp.br)
  • Epithelial tissue is one of the four major types of tissue found in animals, and is the only type of tissue that is able to form and maintain layers of cells that are just one cell thick. (elifesciences.org)
  • Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
  • After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • Within the host cell, viral RNA triggers the activation of the RIG-I signaling pathway, leading to the production of IFN-I. Because an exaggerated IFN-I response causes severe tissue damage, RIG-I signaling is tightly regulated. (bvsalud.org)
  • The tissue of origin for renal cell carcinoma (RCC) is the proximal renal tubular epithelium. (medscape.com)
  • In conclusion we've applied an over-all guideline to evaluate the suitability of targets for disseminated tumor cells: the concomitant consideration of antigen abundance specificity and functional importance along with Vatiquinone the use of tumor cells disseminated from the. (bibf1120.com)
  • Chimeric monoclonal antibodies activate antigen-presenting cells (APCs) and T cells more effectively than murine monoclonal antibodies but can still induce production of human anti-chimeric antibodies. (msdmanuals.com)
  • Cannabinoids are a class of pharmacologic compounds that offer potential applications as antitumor drugs, based on the ability of some members of this class to limit inflammation, cell proliferation, and cell survival. (aacrjournals.org)
  • In fact, low levels of telomerase activity have been found in human adult stem cells including haematopoietic and non-haematopoietic stem cells such as neuronal, skin, intestinal crypt, mammary epithelial, pancreas, adrenal cortex, kidney, and mesenchymal stem cells (MSCs) ( Table 1 ). (nature.com)
  • Appropriate and rapid responses to external Acemetacin (Emflex) stimuli are crucial for maintenance of genome cell and integrity survival. (bio2009.org)
  • Since 2015, more than 3,000 articles were published on studies using crispr /Cas9 genome editing, including more than 900 articles using the technology in mammalian cells (PubMed, accessed June 11, 2017). (brill.com)
  • [ 7 , 8 ] Sister chromatid exchanges are considered a sensitive indicator for cell genome instability, as they are thought to be the outcome of DNA double-strand breaks resulting from homologous recombination repair. (medscape.com)
  • Nucleoside diphosphate kinase: role in bacterial growth, virulence, cell signalling and polysaccharide synthesis. (embl.de)
  • Specific examples of the role of Ndk in regulating growth, NTP formation and cell surface polysaccharide synthesis in two respiratory tract pathogens, Pseudomonas aeruginosa and Mycobacterium tuberculosis, are discussed. (embl.de)
  • This allowed the advancement of research on metabolic and genetic features of the clone cell. (wikipedia.org)
  • This process enables tumor cells, including those within the core of the heterogeneous tumor mass to survive, despite being at a distance from blood vessels that supply their metabolic needs [ 16 , 17 ]. (hindawi.com)
  • In the metabolic symbiosis model, these cells have been proposed to overexpress MCT4 to release high amounts of lactate produced during anaerobic glycolysis into the tumor microenvironment. (hindawi.com)
  • By performing ex vivo experiments on circulating cells from Sézary patients, we established that KIR3DL2 was functional on tumor cells and acted as an inhibitory co-receptor. (hipi-lab-saint-louis.fr)
  • While much is known about the ErbB receptor binding characteristics of BTC and its effect on a variety of cultured cells under different conditions, the challenge that lies ahead is to determine the role of BTC in vivo. (embl.de)
  • Single cells separated out required the support of other cells in order to function and proliferate. (wikipedia.org)
  • Hematopoietic stem cell transplantation remains the only curative therapeutic approach that reliably results in resolution of bone marrow fibrosis in patients with myelofibrosis. (haematologica.org)
  • 3 Myelofibrosis (MF) refers to the Philadelphia chromosome ( BCR-ABL1 )-negative myeloproliferative neoplasm (MPN) originating at the level of the multipotent hematopoietic stem cell. (haematologica.org)
  • Outcomes Just the EpCAM+ inhabitants of cells in pleural effusions can be tumorigenic The pleural effusion can be a complex combination of immune system cells mesothelial cells fibroblasts and tumor cells. (bibf1120.com)
  • Despite advances in surgical techniques and multimodal treatment regimens with radio-, chemo-, and immunotherapy [ 3 - 5 ], the prognosis for malignant glioma patients is very poor with a median survival time of approximately 15 months after diagnosis [ 6 , 7 ]. (hindawi.com)
  • As such we plan to eventually employ the therapeutic mAbs in combination with chemotherapy as a means of enhancing the immunogenicity of the tumor system being treated and to possibly weaken the malignant growth for easier destruction by the mAb. (jcancer.org)
  • Subsequently, two ubiquitin-like conjugation systems, ATG5/ATG12 and ATG8, the mammalian homologues of which include LC3, GABARAP, and GATE-16, concert the formation of the double-membraned autophagosome [ 9 ]. (hindawi.com)
  • The first human, trophoblastic endocrine cell type to be maintained in continuous culture. (sigmaaldrich.com)
  • Recently, the importance of telomere maintenance in human stem cells has been highlighted by studies on dyskeratosis congenital, which is a genetic disorder in the human telomerase component. (nature.com)
  • The regulation of telomere length and telomerase activity is a complex and dynamic process that is tightly linked to cell cycle regulation in human stem cells. (nature.com)
  • Here we review the role of telomeres and telomerase in the function and capacity of the human stem cells. (nature.com)
  • The image depicts a curled up infant-like human, now referred to as a homunculus, inside the head of a sperm cell. (asu.edu)
  • Consequently, we right here current the cloning and functional characterization in the guinea pig Survivin and carried out a functional comparison with the human orthologue. (mirnamimic.com)
  • This clone is cross reactive with non-human primate. (bio2009.org)
  • Komor, Badran and Liu, 2017 ) have opened up tremendous opportunities for the development of cell lines, especially those of human origin ( Tobita, Guzman-Lepe and de L'Hortet, 2015 ). (brill.com)
  • Substitutions at 148 and 151 arise from virus propagation in laboratory cell cultures, whereas changes at 150 occurred during virus evolution in the human host. (cdc.gov)
  • Sanford developed micropipettes where single cells could be picked up and isolated from under the microscope and placed in a detailed microenvironment, where diffusion of cellular products was restricted to inside a small closed culture. (wikipedia.org)
  • Basal macroautophagy (thereafter referred to as autophagy), a catabolic recycling system in cells, is key to maintaining cellular homeostasis and survival. (hindawi.com)
  • Telomerase can add telomeric repeats onto the chromosome ends, and prevents the replication-dependent loss of telomere and cellular senescence in highly proliferative cells of the germline and in the majority of cancers ( Blasco, 2005 ). (nature.com)
  • Poly(ADP-ribosyl)ation plays a key role in a wide range of cellular responses including DNA repair chromatin modification control of transcription and cell death [7-9]. (bio2009.org)
  • Despite the fact that we analyzed all EST clones covering part of the BCLL sequence, we targeted our review on these clones which have both MG-132 clinical trial selleck insertions or deletions in comparison with previously cloned BCLL mRNA variants , as a way to exclude sequences derived from genomic DNA contamination. (mirnamimic.com)
  • Deletions of 3p occur commonly in renal cell carcinoma associated with von Hippel-Lindau disease. (medscape.com)
  • During the interphase of early embryonic cell cycles, the OHO31 protein is present in the cytoplasm and massively accumulates in the nucleus at the onset of mitosis in late interphase and prophase. (rupress.org)
  • check the tag ADOLESCENCE HN - 2008 BX - Nutrition in Adolescence FX - Adolescent Nutrition Physiology MH - Peritoneal Stomata UI - D054048 MN - A01.047.025.600.700 MN - A10.810 MS - Natural openings in the subdiaphragmatic lymphatic plexus in the PERITONEUM, delimited by adjacent mesothelial cells. (bvsalud.org)
  • however, the level of telomerase activity is low or absent in the majority of stem cells regardless of their proliferative capacity. (nature.com)
  • As stem cells have elongated proliferative capacity, they should have a mechanism that maintains telomere length through many cell divisions. (nature.com)
  • Stem cells are undifferentiated cells that are capable of dividing for long periods of time and can give rise to specialized cells under particular conditions. (asu.edu)
  • Embryonic stem cells are a particular type of stem cell derived from embryos. (asu.edu)
  • Thus, telomerase activity and telomere maintenance are associated with the immortality of cancer cells, germ-line cells, and embryonic stem (ES) cells. (nature.com)
  • We demonstrate that the transformation of breast cancer cells is dependent on PVRL4. (elifesciences.org)
  • This thesis aimed to 1) lower the cost of production by using cheap C1 carbon sources (e.g. methane) from agricultural activities (e.g. intensive dairy, piggeries, etc.) for the cultivation of transformed yeast and 2) to assess the therapeutic efficacy of recombinant hIFNγ in its glycosylated and non-glycosylated form from different expression systems against ovarian cancer cells. (edu.au)
  • Experimental treatment approaches include vaccines and nonmyeloablative allogeneic peripheral blood stem cell transplantation. (medscape.com)
  • Our results further indicate that MCT4 promotes proliferation and survival by altered cell cycle regulation and cell death mechanisms. (hindawi.com)
  • Possible mechanisms could include lower dependence on BCR signals of leukemic cells with particular combinations of genetic lesions and/or adoptive changes in thekinomeresultingingreateractivationofcompensatorypathwaysthatsubstitutefortheBCRsignal. (unicatt.it)
  • RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (lookformedical.com)
  • Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. (lookformedical.com)
  • For this purpose, we designed an MCT4 overexpression and knockdown/inhibition model to explore the functional consequences of MCT4 expression on cancer cell metabolism, survival, proliferation, migration, invasion potential, and angiogenesis as well as on cell cycle profiles and cell death mechanisms. (hindawi.com)
  • For these reasons developing experimental strategies that could be used to understand, identify and predict mechanisms of resistance in different malignant cells would be a major advance. (biomedcentral.com)
  • This cost-effective approach can be readily applied to different cell lines, to identify canonical or context specific resistance mechanisms. (biomedcentral.com)
  • Cells have various mechanisms to restore length (TELOMERE HOMEOSTASIS. (lookformedical.com)
  • Although malignant cells were identified as a clonal expansion of CD4+ T cell with memory phenotype, their phenotypic detection relies on the loss of pan-T cell antigens such as CD26 or/and CD7. (hipi-lab-saint-louis.fr)
  • In addition, the following are also involved: antimicrobial peptides, natural antibodies, the complement system, NK cells, and gamma delta T lymphocytes [ 3 ] . (encyclopedia.pub)
  • We examined the effect of amiloride on the activity of recombinant GABARs expressed transiently in L929 fibroblasts to determine its mechanism of action on mammalian GABARs and to determine whether the subunit composition of the receptor influenced its sensitivity to amiloride. (aspetjournals.org)
  • Her first success in duplicating an identical copy of a cell was with a mouse fibroblast. (wikipedia.org)
  • Chronic lymphocytic leukemia (CLL) is a common B cell malignancy and is the most common type of adult leukemia in western countries. (unicatt.it)
  • Under amino acid starvation, only successfully transformed cells (hIFNγ -HIS4+) can synthesise histidine and therefore thrive. (edu.au)
  • Stable F98 glioma cell lines with MCT4 overexpression, normal expression, and knockdown were generated. (hindawi.com)
  • Moreover, MCT4 overexpression enhances cell migration and invasiveness via reorganization of the actin cytoskeleton. (hindawi.com)
  • Although bone marrow fibrosis is seen in a variety of malignant and non-malignant disease states, the deposition of reticulin and collagen fibrosis in the bone marrow of patients with myelofibrosis is believed to be mediated by the myelofibrosis hematopoietic stem/progenitor cell, contributing to an impaired microenvironment favoring malignant over normal hematopoiesis. (haematologica.org)
  • PV is a myeloproliferative neoplasm (MPN) of the bone marrow characterized by an overproduction of erythrocytes and often other blood cells. (cdc.gov)