Mammalian cell vero cells. Medical search. Frequent questions

Research strains transfected with viral genes: Vero F6 is a cell transfected with the gene encoding HHV-1 entry protein glycoprotein-H (gH). (wikipedia.org)
Mitochondrial alterations induced by the p13II protein of human T-cell leukemia virus type 1. (microbiologyresearch.org)
These protein subunits can assemble into virus particles without viral RNA genome in mammalian cells to study virus-cell entry 2 and to develop vaccines 3 . (nature.com)
Several receptors have been demonstrated for the interaction of spike (S) protein of SARS-CoV-2 with host cells, including angiotensin-converting enzyme (ACE2), ephrin ligands and Eph receptors, neuropilin 1 (NRP-1), P2X7, and CD147. (bvsalud.org)
At the beginning of its establishment, KMD Bioscience started from the phage display technology platform to provide high-quality antibody discovery services, and gradually established stable cell line construction and screening, natural protein extraction and fermentation, and recombinant protein custom expression services, a multi-species antibody discovery platform. (kmdbioscience.com)
We use Gibco cell culture medium and related transfection reagents to ensure the high quality and high expression level of each batch of mammalian cell protein expression products. (kmdbioscience.com)
Using this approach, we characterized CoV infection mediated by the SHC014 spike protein in primary human airway cells and in vivo , and tested the efficacy of available immune therapeutics against SHC014-CoV. (nature.com)
Intracellular (immature) virions contain a precursor prM protein, and the cleavage of prM to M happens during the exit of virions from cells. (climat2050.org)
In order to handle these problems and to further test the antigenic variations of the three TBEV subtypes, as well as to provide an optimally folded protein with a similar mammalian glycosylation pattern as with the viral glycoproteins indicated. (climat2050.org)
To date, the function of US11 protein in cell culture and animal models is poorly understood. (biomedcentral.com)
Western blot analysis demonstrated that the US11 protein was specifically recognized by the polyclonal antibody, and immunofluorescent assay also showed that the antibody was able to probe the US11 protein in the cells infected with HSV-1. (biomedcentral.com)
In the present study, we obtained a high-level expression of the recombinant US11 protein as well as high titers of rabbit polyclonal antibody specially against US11 protein in HSV-1 infected cells. (biomedcentral.com)
Finally, US11 has been recently shown to also counteract the activity of the 2'-5' oligoadenylate synthetase (OAS), a cellular protein critical for host cell defense [ 15 ]. (biomedcentral.com)
The His-tagged US11 protein was then expressed in E. coli BL21 (DE3) cells and purified by a nickel-nitrilotriacetic acid (Ni 2+ -NTA) affinity resin under denaturing conditions. (biomedcentral.com)
An additional adhesin, BBK32 (fibronectin binding protein) is a surface lipoprotein that can bind both fibronectin and GAGs to promote binding of B. burgdorferi to various mammalian cells [41, 53]. (phosphatases.com)
The severe acute respiratory syndrome coronavirus (SARS-CoV) 7a protein, which is not expressed by other known coronaviruses, can induce apoptosis in various cell lines. (doctortarget.com)
We hypothesized that SARS-CoV encodes a small envelope E protein that is homologous with MHV E protein, thus inducing T-cell apoptosis. (doctortarget.com)
Our results showed that SARS-CoV E protein induced apoptosis in the transfected Jurkat T-cells, which was amplified to higher apoptosis rates in the absence of growth factors. (doctortarget.com)
The present disclosure provides adeno-associated virus (AAV) virions with altered capsid protein, where the AAV virions exhibit greater infectivity of retinal cells compared to wild-type AAV. (justia.com)
Being typically a minor component of the cell membrane, they are of utmost importance for biological functions that rely on lipid-lipid and lipid-protein interactions (Schnaar and Kinoshita 2015 ). (springer.com)
Protein demand and, thus, the demand for cell cultures in protein production fluctuate. (biomedcentral.com)
Hantaviral N protein was detected in tubular and glomerular cells and cell-to-cell contact structures were changed in biopsy samples of patients with serologically confirmed PUUV infections [ 3 ]. (biomedcentral.com)
Confocal immunofluorescent analysis of transgenic A549 cells stably expressing SARS-CoV-2 nucleocapsid (N) protein (A549-N protein) (left, positive) or SARS-CoV-2 envelope (E) protein (A549-E protein) (right, negative), using SARS-CoV-1/2 Nucleocapsid Protein (1C7C7) Mouse mAb (green), DyLight ™ 554 Phalloidin #13054 (red), and DAPI #4083 (blue). (cellsignal.com)
BAG-1 (Bcl-2 associated athanogene-1) is a multifunctional protein, linking cell proliferation, cell death, protein folding, and cell stress. (omeka.net)
Right here we try to ascertain a manufacturing platform of recombinant 38 kDa protein in mammalian cells and to guage the potential worth of 38 kDa protein in TB serodiagnosis. (tbdb.org)

however, viral multiplication was detected in all mammalian cell types by immunohistochemical tests. (cdc.gov)
This is the first pilot-scale mammalian cell-based viral rabies vaccine production study using microcarrier technology reported in Turkey. (researchsquare.com)
Today, Vero cells are considered a more suitable substrate to produce viral vaccines. (researchsquare.com)
VLPs are highly immunogenic and can induce elevated titers of neutralizing antibodies, even without adjuvants, thanks to the maintenance of the native conformation of viral proteins presenting repetitive epitopes to the host immune system cells. (nature.com)
A number of adherent animal cell types are used by vaccine manufacturers, including mammalian and avian lines for both viral and the newer "viral-vectored" vaccines. (cellculturedish.com)
The unique capacity of the viral cyclins to encompass functions of multiple mammalian cyclins probably explains the evolutionary advantage of encoding viral cyclins within the viral genome. (galeriaespacio48.com)
During productive infection, the 152-kb double-stranded HSV-1 genome is rapidly translocated to the nucleus where at least 80 viral genes are transcribed by the host cell RNA polymerase II (Pol II) [ 1 ]. (biomedcentral.com)
US11 has been reported as a potent inhibitor of PKR activation through binding to dsRNA [ 14 ] or through direct interaction with PKR in the context of viral infection [ 12 ] and therefore could interfere with the PKR mediated host cell responses. (biomedcentral.com)
Introduction For some retroviruses, the viral envelope binds to receptors within a pH-independent way, recommending which the virions may fuse towards the cell membrane [1] straight. (angiogenesis-blog.com)
As a result, viral receptors over the cell membrane offer binding sites for the trojan and so are also mixed up in structural modulation of viral envelopes, resulting in the fusion Pseudouridimycin from the viral and mobile membranes and virion entrance, the first step in viral an infection of focus on cells [2]. (angiogenesis-blog.com)
Surprisingly, Bcl-2i also induced the premature apoptosis of cells transfected with viral RNA or plasmid DNA but not of mock-transfected cells. (doctortarget.com)
This viral capsid mediates the ability of AAV vectors to overcome many of the biological barriers of viral transduction-including cell surface receptor binding, endocytosis, intracellular trafficking, and unpackaging in the nucleus. (justia.com)
specific phosphorylation of 5-ethyl-2'-deoxyuridine by herpes simplex virus-infected cells and incorporation into viral dna. (liverpool.ac.uk)
this study compared conventional viral isolation (vi) in cell cultures with a commercial product--virgo antigen detection system--for the identification and typing of herpes simplex virus (hsv). (liverpool.ac.uk)

F6B1.1) HeLa cells Immortalised cell line History and Characterization of the Vero Cell Line -- A Report prepared by CDR Rebecca Sheets, Ph.D., USPHS CBER/OVRR/DVRPA/VVB for the Vaccines and Related Biological Products Advisory Committee Meeting to be held on May 12, 2000 OPEN SESSION www.fda.gov pdf Yasumura Y, Kawakita M (1963). (wikipedia.org)
A variety of primary cells and established cell lines support the replication of flaviviruses: Green monkey (Vero), hamster (BHK-21), human (SW-13, HeLa), porcine (PS), and mosquito cell lines, as well as primary chicken and duck embryo cells have been used for flavivirus isolation and propagation in routine diagnostic applications. (cdc.gov)
Experience with the culture and transfection of mammalian cell lines such as HEK293, CHO, HeLa, Vero cells. (peccoud.org)
In addition, the trend up until recently was mostly to use robust and easy to use cells like the HeLa line, which due to its robustness, eliminated the need for deep study and careful training on the delicate nature of animal cell growth. (cellculturedish.com)

a total of 400 randomly selected sheep and goat was screened for the identification of the virus using amplification of B2L gene and transfection of mammalian cells (VERO cells). (biomedcentral.com)

We investigated the in vitro susceptibility of various cell cultures and embryonated eggs to Usutu virus infection. (cdc.gov)
Cell infection, at an MOI of 0.3 with serum-free medium conditions, yielded a maximal rabies virus titer of 1.82×10 7 FFU/mL at 5 days. (researchsquare.com)
Finally, all BAC recombinants for cyclin analysis were transfected into Vero-Cre cells for removal of BAC sequences prior to infection of 3T12 fibroblasts for generation of virus stocks. (galeriaespacio48.com)
Additionally, this study revealed distinct genetic complementation groups of the mammalian cyclins, demonstrating that mammalian cyclins can fulfill the biochemical features of the v-cyclin in infection. (galeriaespacio48.com)
Pre-incubation from the recombinant sELR1 with EIAV inhibited EIAV an infection in equine macrophages considerably, the principal in vivo focus on cell from the trojan. (angiogenesis-blog.com)
Taken jointly, our data suggest that sELR1 serves as a secreted mobile aspect that inhibits EIAV an infection in web host cells. (angiogenesis-blog.com)
As opposed to almost every other lentiviruses, such as for example human immunodeficiency trojan (HIV)-1, simian immunodeficiency trojan (SIV) and feline immunodeficiency trojan (FIV), which need co-receptors for effective an infection, EIAV seems to depend just on an operating ELR1 for the invasion of focus on cells. (angiogenesis-blog.com)
tissue colonization and multisystemic disease during mammalian infection, many different types of host cell lines can be employed to investigate Rucaparib manufacturer adherence [58-64]. (phosphatases.com)
Tubular and glomerular cells represent target cells of hantavirus infection. (biomedcentral.com)
We analyzed the cellular consequences of hantavirus infection by measuring adhesion and migration capacity of human renal cells infected with Puumala (PUUV) or Hantaan (HTNV) virus. (biomedcentral.com)
Infection of kidney cells with hantavirus species PUUV and HTNV causes a significant reduction of migration capacity. (biomedcentral.com)
The direct impairment of migration capacity of renal cells by hantaviral N proteins may contribute substantially to proteinuria observed in the clinical picture of hantavirus infection. (biomedcentral.com)
The infection of renal cells by hantaviruses may contribute to proteinuria and may represent a determinant in the clinical course of HFRS. (biomedcentral.com)
However, the underlying mechanisms and effects of hantavirus infection on human renal cells have not been investigated so far. (biomedcentral.com)
To identify the consequences of hantavirus infection, we analyzed adhesion and motility capacity of human renal cells infected with hantaviruses PUUV or HTNV. (biomedcentral.com)
infection of human epidermoid carcinoma no. 2 cells with herpes simplex virus type 1 (hsv-1) leads to a reorganization of antigens associated with both the small and heterogeneous nuclear ribonucleoprotein complexes (snrnp and hnrnp). (liverpool.ac.uk)
Thus, CD4 T cell assist performs a vital function in producing protecting CD8 T cell responses towards M. tuberculosis an infection in vitro and in vivo. (tbdb.org)

Further, VLPs have the advantage that, contrary to inactivated or attenuated viruses that must be prepared in mammalian cell lines, they can be produced in heterologous systems, such as bacteria, yeasts, insect cells, or plants. (nature.com)
We have developed a specific and sensitive -capture IgM immunoassay (11) based on secreted recombinant TBEV precursor membrane envelope (prME) antigens produced in insect cells. (climat2050.org)

Many animal and human vaccines are produced in attachment-dependent cells such as VERO, MDCK or chick embryo fibroblast (CEF) cells. (cellculturedish.com)
Only three cell lines have been studied for the manufacture of influenza vaccines: Vero cells, PER.C6 cells, and Madin-Darby Canine Kidney (MDCK) cells. (substack.com)
Flucelvax Quadrivalent is the only influenza vaccine approved for use in the US made from mammalian MDCK cells (Madin-Darby Canine Kidney). (substack.com)
MDCK cells were extracted from dog kidneys in 1958 and have been used in perpetuity for more than 60 years. (substack.com)

GenIbet runs projects covering a broad spectra of Biopharmaceuticals development, including Recombinant proteins, Vaccines, RNA, Live Microbial Products and Cell and Gene Therapy products. (asgct.org)
IgM and IgG immunofluorescence assays (IFAs) were created based on Vero E6 cells transfected with the recombinant plasmid transporting the TBEV Karelia-94 prME glycoproteins. (climat2050.org)
Despite the advantages of this Dilmapimod assay, the antigen titer in the cell tradition supernatant was not high, and the presence of viable recombinant baculoviruses in the antigens entail the need for permits to work with genetically modified organisms (GMO) or unique safety precautions to use GMO-contaminated antigen. (climat2050.org)
In modern biotechnology and drug design large-scale cell cultures are necessary tools for the production of diverse recombinant proteins, such as Herceptin™, Enbrel™ or vaccines against the influenza virus strains H5N1 and H1N1 like Celvapan™ [ 1 - 7 ]. (biomedcentral.com)
Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable. (cellsignal.com)
Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.com)
Recombinant lentiviral vector LV-CMV-38 kDa-eGFP was packaged, titered, after which transduced into HEK 293 T cells. (tbdb.org)
Recombinant cell traces have been chosen by limiting dilution. (tbdb.org)

The EA.hy926 cell line was derived from human umbilical vein endothelial cells, and it has been shown to express differentiated functions that are characteristics of human vascular endothelium [66, 67]. (phosphatases.com)
Similar Ca 2+ and phosphate concentrations did not increase cold-induced cell injury in the kidney cell line LLC-PK 1 , porcine aortic endothelial cells or rat hepatocytes. (biomedcentral.com)
However, more extreme conditions (Ca 2+ was nominally absent and phosphate concentration raised to 25 mM as in the organ preservation solution University of Wisconsin solution) also increased cold-induced injury in rat hepatocytes and porcine aortic endothelial cells. (biomedcentral.com)
Human renal glomerular endothelial cells (HRGEnC) (ScienCell) were maintained in endothelial cell medium ECM (ScienCell). (biomedcentral.com)

Furthermore, peripheral blood mononuclear cells (PBMCs) or splenocytes harvested from the vaccinated animals 14 days after the second vaccine dose and analyzed through flow cytometry were used for intracellular cytokine staining assay and enzyme-linked immunospot (ELISPOT) assay to examine the immune responses elicited by the vaccines. (news-medical.net)
May we assume, therefore, that chicken cell substrate vaccines are safe? (substack.com)
VERO cells - in 1998, Baxter Vaccines produced a flu shot called Preflucel that underwent clinical trials in 2008-9. (substack.com)
VERO cells are from African green monkey kidneys, originally used for polio vaccines. (substack.com)
We infer vaccines that elicit each CD4 and CD8 T cells are extra seemingly to achieve success than vaccines that elicit solely CD4 or CD8 T cells. (tbdb.org)

SARS-CoV-2 virions and Vero-E6 cells courtesy of Dr. Mohsan Saeed, National Emerging Infectious Diseases Laboratories (NEIDL), Boston University. (cellsignal.com)

Mammalian cells are a unique tool for in vitro toxicity assays, since they represent, with a fairly high degree of accuracy, the actual biological targets of many environmental pollutants or other compounds with proven or suspected toxicity. (siftdesk.org)
In a broader sense, higher eukaryotic cells have been recruited as biorecognition elements either in advanced cell-based biosensors (CBBs) or in cell-based assays (CBAs) [1]. (siftdesk.org)

Carbamazepine induces mitotic arrest in mammalian Vero cells. (aaem.pl)

Adherent Vero cells can be used in different kinds of microcarriers for industrial production in cell culture type bioreactors. (researchsquare.com)

In Vitro Cell Dev Biol. (aaem.pl)
ASTX was tested in vitro in parasites grown axenically and in co-culture with Vero cells. (parasite-journal.org)
L'ASTX a été testée in vitro sur des parasites cultivés axéniquement et en co-culture avec des cellules Vero. (parasite-journal.org)
Both the cell line and the mode of measurement affected the observed in vitro cellular responses, with each cell type providing a unique pattern. (siftdesk.org)
For in vivo test: In vitro lifestyle and embryo freezing After IVF, oocytes encircled with cumulus cells had been placed in fresh new TCM-199 moderate, as well as the embryos had been co-cultured with cumulus cells, as previously defined [21]. (bioskinrevive.com)
The results indicate that group 2b viruses encoding the SHC014 spike in a wild-type backbone can efficiently use multiple orthologs of the SARS receptor human angiotensin converting enzyme II (ACE2), replicate efficiently in primary human airway cells and achieve in vitro titers equivalent to epidemic strains of SARS-CoV. (nature.com)
Fetal equine dermal (Given) cells are vunerable to EIAV in vitro, as well as the replication of EIAV in Given cells transiently transfected with ELR1-IN was markedly decreased in comparison to replication in cells transfected using the unfilled vector. (angiogenesis-blog.com)
an enzyme linked immunosorbent assay (elisa) system to study specific anti-herpes simplex virus (hsv) antibody production in vitro by human peripheral blood mononuclear cells (pbmc) has been developed. (liverpool.ac.uk)
trifluorothymidine (tft) is known to be concentrated in herpes simplex virus (hsv) infected cells in vitro in the form of phosphorylated derivatives. (liverpool.ac.uk)
Purified helped, however not helpless, CD8 T cells effectively prohibit intracellular bacterial progress in vitro. (tbdb.org)
The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION. (bvsalud.org)

The 'Vero' lineage was isolated from kidney epithelial cells extracted from an African green monkey (Chlorocebus sp. (wikipedia.org)
that binds heparin and dermatan sulfate, and facilitates binding of B. burgdorferi to epithelial cells and glial cells [50]. (phosphatases.com)
For example, Vero cells, which were derived from monkey kidney epithelium [65], can be used as a representative of epithelial cells for studying GAGs-mediated adherence. (phosphatases.com)
Human renal epithelial cells (HREpCs) (PromoCell) were cultured in renal epithelial cell growth medium-2 (PromoCell). (biomedcentral.com)

To 1-day-old confluent monolayers of the permanent cell lines and primary cell cultures, grown on the surface of chamber slides, the Austrian Usutu virus strain Vienna 2001-blackbird (GenBank accession no. (cdc.gov)
Results show that ASTX has some detrimental effects on axenically cultured parasites, but not when cultured with mammalian cell monolayers. (parasite-journal.org)

It binds with cell receptors and mediates virus-cell membrane fusion. (climat2050.org)
2018 ). Here, we will solely focus on the less recognized globosides and in particular the globoside Gb 3 , which is the membrane receptor for vero toxins. (springer.com)
Ultrathin sections of C. albicans treated with 1 μg/ml of WSP1267 showed several ultrastructural alterations, including (a) loss of cell wall integrity, (b) detachment of the plasma membrane from the fungal cell wall, (c) accumulation of small vesicles in the periplasmic region, (d) presence of large electron-dense vacuoles and (e) significantly increased cell size and cell wall thickness. (biomedcentral.com)

As a recent example, CoronaVac, COVID-19 vaccine developed by Sinovac Biotech uses vero cells in production and "Vero" term can be seen on the vaccine container. (wikipedia.org)
The cell culture-based rabies vaccine was established in 1967. (researchsquare.com)
The vaccine industry is completing its move from production in animal and primary tissues (such as embryonated chicken eggs) to cultured cell-based platforms. (cellculturedish.com)

Working stocks are generated from supernatants collected from infected Vero cell lines and stored at -80°C. (jax.org)

Chicken embryo fibroblast cells and chicken embryos were resistant. (cdc.gov)

In vivo , l'ASTX n'a eu aucune valeur thérapeutique contre l'infection aiguë par T. cruzi , utilisée seule ou en association avec NFMX. (parasite-journal.org)

Cyclophilin A (CypA), which has peptidyl-prolyl cis-trans isomerase (PPIase) activity, regulates multiple functions of cells by binding to its extracellular receptor CD147. (bvsalud.org)
Dendritic cell (DC) -specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN), Mannose receptor (MR) and NOD-like receptors (NLRs) associated to inflammasome activation. (tbdb.org)

Glycosphingolipids (GSLs) are a subclass of glycolipids that are found in cell membranes of various organisms ranging from bacteria to humans. (springer.com)
Secondly, the low sensitivity and specificity of available polyP detection methods make it difficult to confidently ascertain polyP presence in mammalian cells, since in higher eukaryotes, polyP exists in lower amounts than in yeast or bacteria. (silverchair.com)

The fibroblast-like synovial cell in rheumatoid arthritis: a key player in inflammation and joint destruction. (aaem.pl)
Fibroblast-like synoviocytes: key effector cells in rheumatoid arthritis. (aaem.pl)
We present a novel approach for assessing pesticide toxicity on mammalian cells, by measuring the response patterns of four cell lines (two neuroblastoma and two fibroblast) to three different pesticide groups (carbamates, organophosphates, pyrethroids) at a broad range of concentrations. (siftdesk.org)

Vero F6 was transfected via a concatenated plasmid with the gH gene after a copy of the HHV-1 glycoprotein-D (gD) promoter region. (wikipedia.org)

Vero cells are a lineage of cells used in cell cultures. (wikipedia.org)
We investigated the susceptibility to Usutu virus ( Flavivirus ) of 13 permanent cell lines, 3 primary cell cultures, and chicken embryos. (cdc.gov)
In modern biotechnology, there is a need for pausing cell lines by cold storage to adapt large-scale cell cultures to the variable demand for their products. (biomedcentral.com)
This would allow a more flexible handling of cell cultures adapted to the demand, e.g. a rapid upscaling of cultures after storage, i.e. keeping cells in "stand-by" storage. (biomedcentral.com)

Manual of selected cultured cell lines for bioscience and biotechnology (in Japanese). (wikipedia.org)
Still, the vast number of pollutants of interest has prohibited the rapid development of cell lines with selective biorecognition elements. (siftdesk.org)
We have used these cell lines to compare the differential adherence abilities of N40D10/E9 and B31 strains. (phosphatases.com)
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). (bvsalud.org)

Chromosome 12 of Vero cells has a homozygous ~9-Mb deletion, causing the loss of the type I interferon gene cluster and cyclin-dependent kinase inhibitors CDKN2A and CDKN2B in the genome. (wikipedia.org)
The modified S-trimer was produced by synthesizing and cloning the codon-optimized gene into a mammalian expression vector. (news-medical.net)
The present disclosure further provides methods of delivering a gene product to a retinal cell in an individual, and methods of treating ocular disease. (justia.com)
Gene delivery of secretable neuroprotective factors to Müller cells, a type of retinal glia that contacts all classes of retinal neurons, represents an ideal approach to mediate protection of the entire retina. (justia.com)

Vero E6 cells show some contact inhibition, so are suitable for propagating viruses that replicate slowly. (wikipedia.org)

Transgenic A549 cells were generously provided by Dr. Elena Piskounova, Weill Cornell Medicine. (cellsignal.com)

In addition, fluorescence microscopy of cells labelled with Nile Red showed an accumulation of lipid droplets in the cytoplasm of treated yeasts. (biomedcentral.com)

Here we show that the ion channel activity of HCV p7 expressed in mammalian cells can substitute for that of influenza virus M2 in a cell-based assay. (microbiologyresearch.org)

The whole genome sequence of a Vero cell line was determined by Japanese investigators in 2014. (wikipedia.org)
The genome analysis indicated that the Vero cell lineage is derived from a female Chlorocebus sabaeus. (wikipedia.org)

3) certain enveloped viruses express hemagglutinin esterase (HE), an enzyme that releases these glycan-mediated bindings to host cells, which is expressed among betacoronaviruses in the common cold strains but not the virulent strains, SARS-CoV, SARS-CoV-2 and MERS. (bvsalud.org)
Firstly, the mammalian enzyme responsible for the synthesis of this linear biopolymer is unknown. (silverchair.com)
However, in contrast with what was previously demonstrated [ 9 ], we recently reported that D. discoideum cells lacking DdPpk1 have no detectable polyP levels demonstrating that this is the sole enzyme responsible for polyP synthesis in social amoeba [ 10 ]. (silverchair.com)

The original cell line was named Vero after an abbreviation of verda reno, which means 'green kidney' in Esperanto, while vero itself means 'truth' in Esperanto. (wikipedia.org)
as host cells for eukaryotic parasites, specially of the trypanosomatids Vero (ATCC No. CCL-81) Isolated from C. aethiops kidney on 27 Mar 1962 Vero 76 (ATCC No. CRL-1587) Isolated from Vero in 1968, it grows to a lower saturation density (cells per unit area) than the original Vero. (wikipedia.org)
After that, we examined the result of cultured conditioned mass media (CM) of IVF embryos on splenic immune system cells and Madin-Darby bovine kidney (MDBK) cells with stably presented ISG15 promoter-reporter constructs. (bioskinrevive.com)
We compared various cell culture media/solutions for cold storage of Vero-B4 kidney cells, a cell line widely used in biotechnology. (biomedcentral.com)

These data suggest that the combination of low calcium and high phosphate concentrations in the presence of glucose enhances cold-induced, iron-dependent injury drastically in Vero-B4 cells, and that a tendency for this pathomechanism also exists in other cell types. (biomedcentral.com)

Matured oocytes had been inseminated with frozen-thawed semen from order MK-0822 a Japanese Dark bull (altered to 2 107 cells/ml) for 5 h at 38.5C within a humidified atmosphere with 5% CO2 in the surroundings, in 1 ml of BO solution containing 10 mg/ml bovine serum albumin and 10 g/ml heparin. (bioskinrevive.com)

Rouleaux (stacked clumps) of red blood cells (RBCs) observed in the blood of COVID-19 patients in three studies call attention to the properties of several enveloped virus strains dating back to seminal findings of the 1940s. (bvsalud.org)

In conclusion, Vero cells were grown on Cytodex 1 with serum-free media and a high amount of rabies virus was obtained. (researchsquare.com)
Flublok contains purified HA proteins produced in an insect cell line derived from Sf9 cells of the fall armyworm, Spodoptera frugiperda, and then grown in a medium composed of chemically-defined lipids, vitamins, amino acids, and mineral salts. (substack.com)

Here we show that small-molecules, which inhibit cellular anti-apoptotic Bcl-2 proteins (Bcl-2i), induced the premature death of cells infected with different RNA or DNA viruses, whereas, at the same concentrations, no toxicity was observed in mock-infected cells. (doctortarget.com)
2 SARS-CoV2 cell entry is not complex Fig. 1 is a schematic illustration of the minimum proteins required to mediate the SARS-CoV2 cell entry. (pubannotation.org)
Fig. 1 Proteins required for the SARS-CoV2-cell entry. (pubannotation.org)

In Vero lineage F6, expression of gH is under the control of the promoter region of gD. (wikipedia.org)
FIGS. 10A-D depict expression of ShH10.Y445F.scCAG-GFP in Müller cells after intravitreal injection. (justia.com)
After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.com)

We show synergy between CD4 and CD8 T cells in selling the survival of contaminated mice. (tbdb.org)

Adenovirus DNA has been combined with the retinal cells, creating what is referred to as a "designer substrate," a cell complex that can replicate indefinitely. (substack.com)

Cells were propagated in Earle's minimal essential medium (MEM) (Gibco Invitrogen, Paisley, UK) containing L-glutamine, antimicrobial drugs, and 10% fetal calf serum (FCS). (cdc.gov)

Experiments were conducted on human synoviocytes derived from an RA patient and rabbit synoviocytes cell line HIG-82. (aaem.pl)
Effects of valproic acid (VPA) and levetiracetam (LEV) on proliferation, apoptosis and hormone secretion of the human choriocarcinoma BeWo cell line. (aaem.pl)
Stander M, Dichgans J, Weller M. Anticonvulsant drugs fail to modulate chemotherapy-induced cytotoxicity and growth inhibition of human malignant glioma cells. (aaem.pl)
TBEV is the most important human being pathogen of the mammalian tick-borne group of the genus within the family (3). (climat2050.org)
These cells, thought to be responsible for introducing the cancer-causing Simian Virus 40 (SV40) into the human population, are still in use today. (substack.com)
The T/C-28a2 cell line was developed from human chondrocyte cells [69], which were shown to express fibronectin, decorin and dermatan sulfate [70, 71]. (phosphatases.com)
The human podocyte cell line was derived from human normal podocytes conditionally transformed with a temperature-sensitive mutant of the simian virus 40 (SV40) large T antigen. (biomedcentral.com)
it was reported that glycoprotein c of hsv-1 (gc-1) mediates the binding of c3b-coated erythrocytes to infected cells and has regulatory (decay-accelerating) activity for the alternative pathway c3 convertase of human complement. (liverpool.ac.uk)
However, the SARS-CoV 2 S entry raised for VeroE6 cells and remained high in BHK cells that express human ACE. (pubannotation.org)

to inhibit the actions of cytokines made by Th1 immune system cells.9 Moreover, a direct impact of just one 1,25(OH)2D3 on B-cell homoeostasis Mouse monoclonal to SNAI1 has been verified.10 Therefore, supplement D may have a job in B-cell-related autoimmune disorders such as for example SLE. (informationalwebs.com)
Reports claim that sufferers with SLE possess modifications in steroid hormone fat burning capacity.11 It's been demonstrated Nifuroxazide that oestrogen results are mediated through oestrogen receptors and probably , that are expressed in an array of immune system cells and so are involved with adaptive and innate immune system responses. (informationalwebs.com)

Fundamental Techniques in Cell Culture: a Laboratory Handbook. (wikipedia.org)
All cell culture conditions and virus growing kinetics in serum-free media were investigated. (researchsquare.com)
In the formative years of cell culture, many referred to it as more art than science. (cellculturedish.com)
Most likely, a response to the finicky nature of cells, inconsistent results and a lack of understanding about the science behind cell culture. (cellculturedish.com)
As technological advancements occurred in cell culture, things like prepared media, biological safety cabinets, and disposable plasticware, scientists had time to think about other things beyond the best cell culture practices. (cellculturedish.com)
In the mid-1990s, the WHO began recommending the use of a cell culture alternative to the egg-based production process. (substack.com)
Cold storage in RPMI 1640 medium, a recommended cell culture medium for Vero-B4 cells, surprisingly, strongly enhanced cold-induced cell injury in these cells in comparison to cold storage in Krebs-Henseleit buffer or other cell culture media (DMEM, L-15 and M199). (biomedcentral.com)
the system is designed to identify and type hsv from direct lesion smears (ds) and from cell culture smears (cs) infected with a swab from the patient's lesion by means of an indirect fluorescence assay (ifa) using hsv type-common and hsv-2 type-specific monoclonal antibodies. (liverpool.ac.uk)

At present, the insect-expressing cells commonly used by KMD Bioscience are SF9, and the medium is Gibco in order to provide customers with high-quality products. (kmdbioscience.com)

Bioelectric assay methods based on mammalian cells have gained considerably in popularity over the past few years. (siftdesk.org)

Vero E6 cells were maintained in DMEM (Capricorn) supplemented with 10% FCS. (biomedcentral.com)

Here, topics are discussed that should be considered when converting a promising preclinical finding into a scalable process that could be used for the commercial scale manufacture of cell-based products. (cellculturedish.com)

Two different aspects of the cellular bioelectric response are measured, namely the potential and the combined resistance + capacitance of the cell suspension. (siftdesk.org)

For the analysis of GSLs, they are extracted from tissues and cells using organic solvents. (springer.com)

Vero E6, also known as Vero C1008 (ATCC No. CRL-1586) This line is a clone from Vero 76. (wikipedia.org)
While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.com)

Anatomy32

Organisms47

Diseases2

Chemicals and Drugs76

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes69

Information Science4

Health Care2

Protein25

Viral14

HeLa4

Transfection1

Infection17

Insect cells2

MDCK4

Recombinant8

Endothelial cells4

Vaccines5

Virions1

Assays2

Induces1

Bioreactors1

Vitro11

Epithelial cells4

Monolayers2

Membrane3

Vaccine3

Supernatants1

Embryo fibroblast1

Vivo1

Receptor2

Bacteria2

Fibroblast3

Plasmid1

Cultures4

Lines4

Gene4

Contact inhibition1

A5491

Yeasts1

Influenza1

Genome2

Enzyme3

Kidney4

Phosphate1

Bovine1

Strains1

Grown2

Proteins3

Expression3

Mice1

Retinal cells1

Fetal1

Human9

Immune system cells2

Culture8

Commonly1

Considerably1

DMEM1

Manufacture1

Suspension1

Analysis1

Clone2