Within this assay RNA minigenomes, i.e., small variations from the EBOV genome with all viral genes changed and taken out using a reporter gene, are expressed in mammalian cells using the viral RNP protein together. (mergullo.net)
SHEEP ANTI-CHIKUNGUNYA VIRUS Sheep anti Chikungunya virus antibody has been raised against recombinant CHIKV VLPs, expressed in mammalian cells. (maxanim.com)
LCMV3
Heretofore the only arenaviruses known to occur in North America were LCMV, WWAV, and TAMV. (cdc.gov)
A single inoculation of a plasmid encoding full-length Lassa nucleoprotein (LNP) can induce CD8 (+) T cell responses in mice and protect them against infection with two arenaviruses, LCMV and Pichinde virus (PV). (helixbiogeninstitute.org)
Genome2
Arenaviruses have an RNA genome which is not known to be copied into DNA at any stage of the replication cycle. (virology.ws)
Therefore the contribution of non-retroviral RNA viruses to evolution of the mammalian genome might be greater than previously believed. (virology.ws)
Viruses4
BackgroundRodent-borne viruses such as orthohantaviruses and arenaviruses cause considerable disease burden with regional and temporal differences in incidence and clinical awareness. (bvsalud.org)
Dr. Cross also has a long standing interest in the epidemiology and ecology of these viruses where a particular focus has been centered on mammalian reservoirs of viral infection. (utmb.edu)
The matrix Z proteins of arenaviruses are related to cellular RING domain proteins, and the matrix proteins of some negative strand RNA viruses are related to cellular cyclophilin. (virology.ws)
Insect cell-based protein expression is a low-cost method for solubilizing and folding proteins of mammalian origin while retaining their antigenic, immunogenic, and functional properties. (helixbiogeninstitute.org)
It reduces the degradation of ubiquitin-conjugated proteins in mammalian cells and permeable strains of yeast by the 26S complex without affecting its ATPase or isopeptidase activities. (xcessbio.com)
Recombinant1
Mammalian expressed recombinant MXRA8 protein, the cell surface receptor for alphaviruses. (thenativeantigencompany.com)
Viral2
Zuckerman 2014, p. 10 "Viral Hemorrhagic Fevers Caused by Arenaviruses" (PDF). (wikipedia.org)
Dr. Cross' research is centered on the biology and pathogenesis of high priority viral agents with a particular emphasis on elucidating mechanisms of vascular leak, coagulopathy, and immune derangement in viral hemorrhagic fever infections caused by filoviruses, arenaviruses, and bunyaviruses. (utmb.edu)
Reservoirs1
Cercariae penetrate the skin of humans or, in the case of S. japonicum, humans and other mammalian hosts that act as reservoirs for infection. (wikitropica.org)
Hosts2
Specific rodents are the principal hosts of the arenaviruses for which natural host relationships have been well characterized (2) . (cdc.gov)
We have obtained some electron micrographs showing what appears to be developmental stages of some type of small eukaryotic organism colonizing/parasitizing the tissues of mammalian hosts. (researchgate.net)
Protein2
One example is syncytin , a retroviral protein used for the construction of the mammalian placenta. (virology.ws)
The SARS-CoV-2 protein expression vectors are full-length, and codon optimized for mammalian cell expression. (virongy.com)
Replication2
These events have no counterparts during replication of arenaviruses. (virology.ws)
We talk with Debarpan Dhar, a graduate student in the Miller lab at Iowa State University, who studies packaging and replication of mammalian orthoreoviruses. (podbean.com)
Infectious1
An infectious arenavirus was isolated from 5 of 27 California mice but none of the 7 other rodents. (cdc.gov)
Cells4
A DNA minigene vaccine encoding a 9-amino-acid sequence from LNP also induces CD8 (+) T cells and protects against arenavirus infection, confirming the findings. (helixbiogeninstitute.org)
2',3'-cGAMP sodium (2'-3'-cyclic GMP-AMP sodium) is a endogenous cGAMP in mammalian cells. (medchemexpress.com)
2',3'-cGAMP sodium is produced in mammalian cells in response to DNA in the cytoplasm. (medchemexpress.com)
Pathway for polyarginine entry into mammalian cells. (xcessbio.com)
Rodents1
Thirty-four rodents captured in southern California were studied to increase our knowledge of the arenaviruses indigenous to the western United States. (cdc.gov)
Humans1
Six arenaviruses are known to cause severe disease in humans (1) . (cdc.gov)
Mice2
Antibody to an arenavirus recently was found in California mice ( Peromyscus californicus ) captured in the Santa Ana Mountains in Orange County, California (9) . (cdc.gov)
The purpose of our study was to determine the identity of the arenavirus associated with such mice in southern California. (cdc.gov)
Cell1
A CMV promoter is used for mammalian cell expression and the backbone contains a selection marker for Geneticin (G418). (virongy.com)
Virus2
Flexal mammarenavirus (also known as the Flexal virus or FLEV, and previously known by the laboratory code BeAn 293022) is a mammarenavirus: an arenavirus with a mammalian host. (wikipedia.org)
Vincent, Alan, and Matt discuss a project to study the RNA virome of Northeastern American bats, failure to detect XMRV in UK chronic fatigue syndrome patients, and DNA of bornavirus, an RNA virus, in mammalian genomes. (microbe.tv)
Guanarito2
Three Z. brevicauda rodent samples had antibody reactive to both Pichindé and Guanarito virus, and 7 more were positive for either Pichindé or Guanarito arenaviruses ( Table ). (cdc.gov)
Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. (cusabio.com)
Infection3
No studies of arenavirus infection in rodents or humans have been conducted in Colombia since 1971. (cdc.gov)
Inhibition of the type I interferon antiviral response during arenavirus infection. (ox.ac.uk)
9. Lassa Virus, but Not Highly Pathogenic New World Arenaviruses, Restricts Immunostimulatory Double-Stranded RNA Accumulation during Infection. (nih.gov)
Mammarenavirus1
Flexal mammarenavirus (also known as the Flexal virus or FLEV, and previously known by the laboratory code BeAn 293022) is a mammarenavirus: an arenavirus with a mammalian host. (wikipedia.org)
Yeast2
These services include Polyclonal Antibody Production, Large-Scale Plasmid Production, DNA Subcloning and Mutagenesis, Protein Production based on E.coli/Yeast/mammalian 293 cell culture, etc. (immune-tech.net)
These proteins of interest can be grown in a variety of host cells such as yeast, bacterial or mammalian cells. (justia.com)
Pathogenesis1
Arenavirus persistence and pathogenesis are facilitated by their ability to overcome the host innate immune response. (ox.ac.uk)
Antibody2
Antibody to an arenavirus recently was found in California mice ( Peromyscus californicus ) captured in the Santa Ana Mountains in Orange County, California (9) . (cdc.gov)
Blood samples were tested for arenavirus immunoglobulin (Ig) G by indirect immunofluorescent antibody assays. (cdc.gov)
Inhibitors1
Nuclear import and export inhibitors alter capsid protein distribution in mammalian cells and reduce Venezuelan Equine Encephalitis Virus replication. (medscape.com)
Mice2
Novel Arenavirus Isolates from Namaqua Rock Mice, Namibia, Southern Africa. (nih.gov)
The purpose of our study was to determine the identity of the arenavirus associated with such mice in southern California. (cdc.gov)
Molecular2
Molecular phylogeny of the arenaviruses. (nih.gov)
Bruno Canard a obtenu un doctorat en 1991 en microbiologie et biochimie de l'Université Paris VII à l'Institut Pasteur (Dir : Dr. Stewart T. Cole), Paris, et une formation post-doctorale en 1995-1998 à la Harvard Medical School, Dept. Biological Chemistry and Molecular Pharmacology (Dir : Dr. Charles C. Richardson), Boston. (univ-mrs.fr)
Shown1
Thus, the arenavirus nucleoprotein (NP) was shown to inhibit the IFN-I response by interfering with the activation of IRF3. (ox.ac.uk)