• Surprisingly within the pgm2/3 lines a sturdy boost in sucrose, at the same time as the starch breakdown derived maltose, was observed. (gardos-channel.com)
  • In the reverse direction to that shown above, the enzyme is specific for alpha,alpha-trehalose as substrate, as it cannot use alpha- or beta-paranitrophenyl glucosides, maltose, sucrose, lactose or cellobiose. (cathdb.info)
  • In enzymology, a maltose phosphorylase (EC 2.4.1.8) is an enzyme that catalyzes the chemical reaction maltose + phosphate ⇌ {\displaystyle \rightleftharpoons } D-glucose + beta-D-glucose 1-phosphate Thus, the two substrates of this enzyme are maltose and phosphate, whereas its two products are D-glucose and beta-D-glucose 1-phosphate. (wikipedia.org)
  • The systematic name of this enzyme class is maltose:phosphate 1-beta-D-glucosyltransferase. (wikipedia.org)
  • Neither a gene encoding a maltose phosphorylase (MalP) nor MalP enzyme activity were detected in the wild type. (frontiersin.org)
  • The hydrolysis products of starch and maltose, analyzed by TLC, demonstrated glucose as end product and confirming the character of the enzyme as glucoamylase. (bvsalud.org)
  • In purine nucleoside phosphorylase deficiency, similar changes occur, resulting in elevated deoxy-GTP (dGTP) levels. (medscape.com)
  • In a further description of the mechanism of T-cell depletion in purine nucleoside phosphorylase deficiency, Arpaia et al reported increased in vivo apoptosis of T cells and increased in vitro sensitivity to gamma irradiation in a murine model. (medscape.com)
  • [ 4 ] The immune deficiency in purine nucleoside phosphorylase deficiency may be the result of inhibited mitochondrial DNA repair due to the accumulation of dGTP in the mitochondria. (medscape.com)
  • See Omenn Syndrome and Purine Nucleoside Phosphorylase Deficiency for a discussion of other forms of SCID. (medscape.com)
  • During fermentation, the microbe consumes maltose and glucose to ultimately release acids that create the sour taste in sourdough. (kenyon.edu)
  • Acid maltase catalyzes the hydrogenation reaction of maltose to glucose. (medscape.com)
  • Dextrins and maltose are converted to glucose by enzymes in the border of the intestinal brush (the microvilli that resemble fingers of the epithelial cells in the intestinal tract, which dramatically increase the surface area of the surface of the intestine). (microbiologynote.com)
  • The glycogen phosphorylase can be converted into glucose 6-phosphate via phosphoglucomutase which is responsible for catalyzing the reversible reaction. (microbiologynote.com)
  • A análise do produto da hidrólise de amido e maltose por TLC, demonstrou que o produto final era glucose, confirmando as características da enzima como glucoamilase. (bvsalud.org)
  • Glycogen that is stored in the tissues of animals (primarily the skeletal muscle and liver) or in microorganisms as well as in the plant tissue, can be used in the cell through a phosphorolytic reaction triggered by the glycogen-phosphorylase (starch the phosphorylase found in plant). (microbiologynote.com)
  • Temperature and pH optima were in the range of 50-60ºC and 5.0-6.0, respectively, using starch and maltose as substrates. (bvsalud.org)
  • The glucoamylase from 15.1 strain showed a Km of 0.094 mg/ml and 0.029 mg/ml and Vmax of 202 U/mg prot and 109 U/mg prot, for starch and maltose, respectively. (bvsalud.org)
  • [ 2 , 3 ] One disorder is adenosine deaminase (ADA) deficiency, which is Online Mendelian Inheritance in Man (OMIM) subject number 102700, and the other is purine nucleoside phosphorylase (PNP) deficiency, which is OMIM subject number 164050. (medscape.com)
  • In both adenosine deaminase and purine nucleoside phosphorylase deficiencies, thymocytes are thought to be selectively destroyed because of elevated levels of dATP and dGTP. (medscape.com)
  • Deficiency of purine nucleoside phosphorylase (PNP) and adenosine deaminase (ADA) elevates intracellular levels of deoxyguanosine and deoxyadenosine, respectively. (medscape.com)
  • Although maltose is an important building block of acarbose, the maltose/maltodextrin metabolism has not been studied in Actinoplanes sp. (frontiersin.org)
  • By this, the maltose/maltodextrin system appears to be fundamentally different from other described prokaryotic systems. (frontiersin.org)
  • Purine nueoside phosphorylase (PNP) deficiency causes a form of severe combined immunodeficiency (SCID) characterized by profound T cell deficiency, failure to thrive (FTT), recurrent deep seeded infection, developmental delay, progressive neurological deterioration, and autoimmune complications. (medscape.com)
  • Maltose metabolism of Lactobacillus sanfranciscensis: cloning and heterologous expression of the key enzymes, maltose phosphorylase and phosphoglucomutase. (wikigenes.org)
  • Essentiality of the Maltase AmlE in Maltose Utilization and Its Transcriptional Regulation by the Repressor AmlR in the Acarbose-Producing Bacterium Actinoplanes sp. (frontiersin.org)
  • By growth experiments and functional assays of Δ amlE , we could show that AmlE is essential for the maltose utilization in Actinoplanes sp. (frontiersin.org)
  • We also report strong association of type 2 diabetes with Maltose phosphorylase (K00691), 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate synthase (K21279) and an uncharacterized protein (K07101). (biomedcentral.com)
  • Fusion partners known to the prior art include maltose binding protein (Di Guan et al. (justia.com)
  • Zhao, M. , Cascio, D. , Sawaya, M. R. , and Eisenberg, D. (2011) Structures of segments of α-synuclein fused to maltose-binding protein suggest intermediate states during amyloid formation. . (anl.gov)
  • Reaction mechanism of chitobiose phosphorylase from Vibrio proteolyticus: identification of family 36 glycosyltransferase in Vibrio. (genome.jp)
  • SE50/110 is the wild type of industrial production strains of the fine-chemical acarbose (acarviosyl-maltose), which is used as α-glucosidase inhibitor in the treatment of type II diabetes. (frontiersin.org)
  • Differences were observed in relation to the products formed with maltose as substrate between the two strains studied. (bvsalud.org)
  • Chitobiose phosphorylase from Vibrio proteolyticus, a member of glycosyl transferase family 36, has a clan GH-L-like (alpha/alpha)(6) barrel fold. (genome.jp)
  • Afshar, S. , Sawaya, M. R. , and Morrison, S. L. (2009) Structure of a mutant human purine nucleoside phosphorylase with the prodrug, 2-fluoro-2'-deoxyadenosine and the cytotoxic drug, 2-fluoroadenine . (anl.gov)
  • It can be assumed, that maltose does not only represent the most important carbon source of Actinoplanes sp. (frontiersin.org)
  • Development and validation of a 2nd tier test for identification of purine nucleoside phosphorylase deficiency patients during expanded newborn screening by liquid chromatography-tandem mass spectrometry. (medscape.com)
  • Immunodeficiency diseases caused by adenosine deaminase deficiency and purine nucleoside phosphorylase deficiency. (medscape.com)
  • Evidence from purine nucleoside phosphorylase-deficient mice. (medscape.com)
  • Adenosine deaminase and purine nucleoside phosphorylase deficiencies: evaluation of therapeutic interventions in eight patients. (medscape.com)
  • Available at https://ghr.nlm.nih.gov/condition/purine-nucleoside-phosphorylase-deficiency . (medscape.com)
  • See Omenn Syndrome and Purine Nucleoside Phosphorylase Deficiency for a discussion of other forms of SCID. (medscape.com)
  • Deficiency of purine nucleoside phosphorylase (PNP) and adenosine deaminase (ADA) elevates intracellular levels of deoxyguanosine and deoxyadenosine, respectively. (medscape.com)
  • The systematic name of this enzyme class is maltose:phosphate 1-beta-D-glucosyltransferase. (wikipedia.org)
  • H. Nakamura, M. Hasegawa, Y. Nomura, K. Ikebukuro, Y. Arikawa, and I. Karube*, "Improvement of a CL-FIA system using maltose phosphorylase for the determination of phosphate-ion in freshwater", Analytical Letters (Taylor & Francis) 2003, 36, 1805-1817 (doi:10.1081/AL-120023615). (nanosociety.us)
  • The family of glycosyl hydrolases which contains this domain includes vacuolar acid trehalase and maltose phosphorylase. (embl.de)
  • S1 RNA binding domain, Ribonuclease E/G family, Polyribonucleotide phosphorylase C terminal [Interproscan]. (ntu.edu.sg)