• ActiveHOOK™ carrier proteins have been pre-activated with the heterobifunctional protein cross linker sulfoSMCC and therefore are ready for the addition of cysteine containing peptides or proteins for a simple one step reaction. (gbiosciences.com)
  • Perform hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens with this sulfhydryl-reactive mariculture KLH. (fishersci.com)
  • Thermo Scientific™ Imject Maleimide-Activated mcKLH is sulfhydryl-reactive immunogenic mariculture keyhole limpet hemocyanin (KLH) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens. (fishersci.com)
  • KLH can be activated with the crosslinker Sulfo-SMCC, which converts lysine residues to sulfhydryl-reactive maleimide groups. (wikipedia.org)
  • The high-quality mariculture KLH is rendered sulfhydryl-reactive by chemical modification with Sulfo-SMCC crosslinker, and the resulting maleimide-activated protein is then stabilized and lyophilized in phosphate buffer. (fishersci.com)
  • Select from among several package sizes of activated KLH protein, or choose the complete conjugation kit that includes optimized buffers and trouble-free spin desalting columns. (fishersci.com)
  • The aminooxy group allows for selective conjugation to aldehydes and ketones, while the carboxylic acid group can be used for further modifications. (axispharm.com)
  • Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein in the production of antibodies for research, biotechnology and therapeutic applications. (wikipedia.org)
  • Its large size and numerous epitopes generate a substantial immune response, and abundance of lysine residues for coupling haptens allows a high hapten:carrier protein ratio, increasing the likelihood of generating hapten-specific antibodies. (wikipedia.org)
  • Immunization of rats with OXY(Gly)(4) conjugated to the carrier proteins bovine serum albumin (BSA) or keyhole limpet hemocyanin (KLH) produced high-titer antibodies to OXY and its metabolite oxymorphone with substantially lower affinities for other structurally related opioid agonists and antagonists. (bvsalud.org)
  • Thorpe, (1985) "Antibody Carriers Of Cytotoxic Agents In Cancer Therapy: A Review," in Monoclonal Antibodies '84: Biological And Clinical Applications, A. Pinchera et al (ed.s), pp. 475-506). (justia.com)
  • Some cytotoxic drugs tend to be inactive or less active when conjugated to large antibodies or protein receptor ligands. (justia.com)
  • Drug moieties used in antibody drug conjugates include bacterial protein toxins such as diphtheria toxin, plant protein toxins such as ricin, small molecules such as auristatins, geldanamycin (Mandler et al (2000) J. of the Nat. (justia.com)
  • There are two keyhole limpet hemocyanin genes, termed KLH1 and KLH2 which share around 60% identity at the protein level. (wikipedia.org)
  • Morphine (M) and oxycodone (OXY) haptens were conjugated to keyhole limpet hemocyanin (KLH) through tetraglycine (Gly)(4) linkers at the C6 position. (bvsalud.org)
  • The KLH protein is potently immunogenic, but does not cause an adverse immune response in humans. (wikipedia.org)
  • Aggregates remain immunogenic, but limit the ability to conjugate haptens, and are difficult to manipulate in the laboratory. (wikipedia.org)
  • It is therefore highly prized as a vaccine carrier protein. (wikipedia.org)
  • Consistent with the enhanced titers in the bivalent group, a trend toward enhanced pharmacokinetic efficacy with the bivalent vaccine was observed. (bvsalud.org)
  • Researchers were able to demonstrate that a cationized form of BSA, produced by replacing anionic side chain carboxylic groups with. (gbiosciences.com)
  • It contains two functional groups - an aminooxy group and a carboxylic acid group, which make it a versatile tool for various applications in pharmaceutical research and development. (axispharm.com)
  • KLH is the most widely employed carrier protein for this purpose. (wikipedia.org)
  • Both encode large glycosylated proteins consisting of around 3400 amino acids and a molecular weight of around 390,000 Daltons, excluding the glycosylation. (wikipedia.org)
  • Screen Quest™ assay kits, a set of HTS-ready tools for high throughput screening of biochemical and cellular targets such as protein kinases, proteases, HDAC, cell apoptosis and cytoxicity, GPCR, ion channels, ADME/metabolism and transporters. (stratech.co.uk)
  • For a full range of protein cross-linkers and accessories, see the 'Modifications & Cross-Linking' section. (gbiosciences.com)
  • The rapidly growing interest in therapeutic vaccines (i.e. active immunotherapies) for cancer and the documented efficacy of KLH as a superior carrier protein for cancer vaccines are creating a significant biopharmaceutical market for KLH formulations. (wikipedia.org)
  • A sulfhydryl-containing hapten can then be reacted with the KLH to complete the immunogen without causing polymerization. (wikipedia.org)
  • The protein oligomerises to form a barrel shaped didecameric complex which is composed of 20 monomers. (wikipedia.org)
  • In the first part, information about the peptide synthesis was given in a very simple and readable format under the title of solid-phase peptide synthesis including a brief history, solid supports, linkers, protecting groups, and analysis method sections. (intechopen.com)
  • In addition, because KLH is derived from the limpet, a gastropod, it is phylogenetically distant from mammalian proteins, thus reducing false positives in immunologically-based research techniques in mammalian model organisms. (wikipedia.org)
  • In addition, we offer HyperCarrier ™ , a cationized BSA that has been treated with ethylene diamine, which substitutes anionic carboxyl groups with cationic aminoethyl-amide groups. (gbiosciences.com)
  • ActiveHOOK™ BSA is maleimide activated BSA, by the addition of sulfoSMCC cross-linker. (gbiosciences.com)
  • iFluor™ superior fluorescent labelling dyes, optimised for labelling proteins and nucleic acids. (stratech.co.uk)
  • a sensitive and robust tool for detection of proteins in solutions and solid surfaces (such as gels and membranes). (stratech.co.uk)
  • Ein empfindlicher Gastrinassay mit Biotinyl-Gastrin Derivaten Zusammenfassung: Es werden zwei N-terminale Biotinyl-Gastrine synthetisiert um den Einfluß der Länge und der chemischen Eigenschaften sowohl auf die Komplexierung des Haptens durch Streptavidin oder Avidin, an Polystyrol adsorbiert, als auch auf die Antigenizität des komplexierten Peptides zu bestimmen. (docksci.com)
  • Key terms: Biotinyl-gastrin, streptavidin biotinyl-hapten complex, enzyme immunoassay. (docksci.com)
  • We report on our efforts to create efficient artificial metalloenzymes for the enantioselective hydrogenation of N-protected dehydroamino acids using streptavidin as host protein. (ward-lab.ch)
  • Haptens are substances with a low molecular weight such as peptides, small proteins and drug molecules that are generally not immunogenic and require the aid of a carrier protein to stimulate a response from the immune system in the form of antibody production. (wikipedia.org)
  • We showed that all TT-hapten conjugates induced high antibody endpoint titers against the targets but only haptens and can induce protective effects against heroin in vivo. (nih.gov)
  • This hapten capture system reduces desorption particularly pronounced for low mass peptides, and avoids possible modifications or suppression of epitopes by the adsorption process with concomitant reduction of antibody binding affinity of the antigen. (docksci.com)
  • We ourselves obtained an artificial hemoprotein by associating a monoclonal antibody, 13G10, and its iron(III)-α,α,α,β-meso-tetrakis(ortho-carboxyphenyl)porphyrin (Fe(ToCPP)) hapten, which exhibited a significant peroxidase activity. (ward-lab.ch)
  • Biological studies suggested that in this antibody, a carboxylic acid side chain of the protein participated in the catalysis, but no amino acid residue acting as an axial ligand of the iron was detected. (ward-lab.ch)
  • Heterobifunctional Reactive groups maleimide and NHS ester Primary amine and sulhydryl reactive Hapten-carrier protein conjugation 1,2 Soluble in DMSO and chloroform Storage: Shipped at ambient temperature, upon receipt store at 4°C. (thomassci.com)
  • M-PEG-2-methylacrylate can be used as a carrier for poorly soluble drugs, which can enhance their absorption and effectiveness in the body. (axispharm.com)
  • Both encode large glycosylated proteins consisting of around 3400 amino acids and a molecular weight of around 390,000 Daltons, excluding the glycosylation. (wikipedia.org)
  • The multidisciplinary nature of this program requires extensive collaboration with other groups with diverse pharmacological and biological expertise from within and outside of NIH. (nih.gov)
  • This new type of assay procedure may also represent a useful tool particularly for epitope mapping with relatively low mass synthetic protein fragments. (docksci.com)
  • M-PEG-2-methylacrylate is a polymer with a unique chemical structure that contains several functional groups. (axispharm.com)
  • The main functional group in M-PEG-2-methylacrylate is the acrylate group, which is a type of double bond that is commonly used in polymerization reactions. (axispharm.com)
  • This functional group allows M-PEG-2-methylacrylate to form long chains of repeating units, which makes it a useful polymer in various industries, including pharmaceutical research and development. (axispharm.com)
  • M-PEG-2-methylacrylate is a unique polymer that contains several functional groups, including the acrylate group, which makes it a useful material in various industries, including pharmaceutical research and development. (axispharm.com)
  • In this paper we summarize the results we obtained on the design, structural, and functional properties of DFs, a family of artificial diiron proteins. (ward-lab.ch)
  • A major objective in protein science is the design of enzymes with novel catalytic activities that are tailored to specific applications. (ward-lab.ch)
  • Metalloprotein mimetics have been developed through the introduction of novel metal-binding sites into naturally occurring proteins as well as through de novo protein design. (ward-lab.ch)
  • Denaturation of KLH also results in a tendency of the protein to aggregate and precipitate from solution. (wikipedia.org)