• We investigated whether isopropanol extraction could facilitate resolution of the less abundant proteins from soybean seeds in one dimensional polyacrylamide gel electrophoresis (1D-PAGE) and two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). (usda.gov)
  • Serum or Plasma Sample Preparation for Two-Dimensional Gel Electrophoresis - Anthony G. Sullivan, Stephen Russell, Henry Brzeski, Richard I. Somiari and Craig D. Shriver 7. (nhbs.com)
  • Large-Format Two-Dimensional Polyacrylamide Gel Electrophoresis - Henry Brzeski, Stephen Russell, Tony G. Sullivan, Richard Somiari & Craig Shriver 14. (nhbs.com)
  • Although migration on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), under denaturing conditions, was unusually slow, molecular weight determination by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) confirmed that the product was consistent with its theoretical size. (ox.ac.uk)
  • Techniques used in human sperm proteome mapping: There are several initial reports using MS proteomics technology to identify a limited number of proteins from the human sperm using twodimensional gel electrophoresis (2-DE) coupled to MALDI-TOF-MS analysis (16 - 23). (jri.ir)
  • The high purity, molecular weight and correct compositions of each new polypeptide have been confirmed via high performance liquid chromatography (HPLC), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and amino acid analysis. (unh.edu)
  • Facilities include a biochemistry laboratory, dedicated clean rooms for high-sensitivity, low-contamination sample handling, fluorescence 2D difference gel electrophoresis (2D-DIGE) capability, liquid-handling robotics and liquid chromatography (LC) for protein and peptide separations. (ucl.ac.uk)
  • Proteome analysis of Arabidopsis thaliana by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionisation-time of flight mass spectrometry. (mpg.de)
  • Evaluation of two-dimensional electrophoresis and liquid chromatography tandem mass spectrometry for tissue-specific protein profiling of laser-microdissected plant samples. (mpg.de)
  • Large-gel two-dimensional electrophoresis-matrix assisted laser desorption/ionization-time of flight-mass spectrometry: an analytical challenge for studying complex protein mixtures. (mpg.de)
  • Addition of 0.1% CYMAL-5 (final concentration) during trypsin treatment was compatible with both matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and liquid chromatography/tandem mass spectrometry (LC/MS/MS) analysis, and gave a better digestion efficiency than n-octylglucoside, which we previously reported. (nih.gov)
  • An extensive human sperm proteome analysis using 1D-SDS-PAGE combined with electrospray liquid chromatography tandem mass spectrometry (GeLC-MS/ MS) approach identified 1,760 proteins (24). (jri.ir)
  • A cycloalkyl aliphatic saccharide, 5-cyclohexyl-1-pentyl-beta-D-maltoside (CYMAL-5), was evaluated as a novel additive in a high-throughput in-gel protein digestion system using 96-well plates. (nih.gov)
  • Detailed protocols involving protein microarrays, liquid chromatography, high throughput cloning of expression constructs, IMAC, mass spectrometry, MALDI TOF, and MudPIT are provided, along with well illustrated descriptions of experimental procedures and lists of recommended Web sites and reading material. (cshlpress.com)
  • Using affinity chromatography by use of monolithic supports with immobilized immunoglobulin binding ligands as a robust tool for isolation of immunoglobulins, IgG can be purified from rat sera. (nsk.hr)
  • We have identified a total of 107 protein spots using Matrix-assisted laser desorption/ionization time of flight mass spectrophotometry (MALDI-TOF-MS) and liquid chromatography mass spectrometry (LC-MS/MS). Our results suggest that extraction of soybean seed powder with 40% isopropanol enriches for lower abundance proteins and is a suitable method for 2D-PAGE separation and identification. (usda.gov)
  • Analysis of Membrane Proteins by Two-Dimensional Gels - Michael Fountoulakis, 15. (nhbs.com)
  • MALDI analysis of proteins after extraction from dissolvable ethylene glycol diacrylate cross-linked polyacrylamide gels. (uni-frankfurt.de)
  • To procure a highly enriched IgG preparation, the fraction with proteins eluted from the column was analysed on a 1D polyacrylamide gel, after which in-gel tryptic digestion of this protein was performed. (nsk.hr)
  • In-gel reduction and alkylation of Cys residues under denaturing conditions also improved the sequence coverage of peptides. (nih.gov)
  • In-gel tryptic digestion with the optimum combination of 0.5 mm thick gels, negative staining, alkylation under denaturing conditions (6 M guanidine hydrochloride), and digestion in the presence of CYMAL-5, gave excellent performance especially for membrane protein analysis, where recovery of hydrophobic peptides was markedly enhanced. (nih.gov)
  • The resulting peptides were successfully identified by MALDI-TOF/TOF mass spectrometry as parts of IgG heavy and light chains. (nsk.hr)
  • Pre-Fractionation of Complex Protein Mixtures for 2-D PAGE Using Reversed Phase Liquid Chromatography Volker Badock and Albrecht Otto 12. (nhbs.com)
  • Xylanase activity of U . maydis cells was indirectly detected by the quantification of released reducing sugars and could be confirmed by visualizing oligosaccharides as degradation products of xylan by thin layer chromatography. (biomedcentral.com)
  • Some proposals were additionally made to address the very common, significant research gaps noted in CECs research, especially the standardization of analytical chemical test methods, based on chromatography-mass spectrometry, for quantification. (openaccesspub.org)
  • These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. (nhbs.com)
  • Gel filtration chromatography yielded an anomalously large Stokes radius, suggesting a fully unfolded conformation. (ox.ac.uk)
  • The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. (nhbs.com)
  • Mass spectrometry (MS) instrumentation for expression profiling, protein/peptide identification and characterisation includes a Thermo LTQ Orbitrap XL hybrid mass spectrometer with on-line Dionex3000 nano-LC system and Bruker Ultraflex MALDI-TOF/TOF instrument. (ucl.ac.uk)
  • Transformation and other factors of the peptide mass spectrometry pairwise peak-list comparison process. (mpg.de)
  • Protein identification by MALDI-TOF-MS peptide mapping: a new strategy. (mpg.de)
  • Linked to an LTQ Orbitrap XL mass spectrometer. (ucl.ac.uk)
  • IUPAC definition ĐM = Mw/Mn where Mw is the mass-average molar mass (or molecular weight) and Mn is the number-average molar mass (or molecular weight). (wikipedia.org)
  • citation needed] IUPAC has deprecated the use of the term polydispersity index, having replaced it with the term dispersity, represented by the symbol Đ (pronounced D-stroke) which can refer to either molecular mass or degree of polymerization. (wikipedia.org)
  • The dispersity (Đ), also known as the polydispersity index (PDI) or heterogeneity index, is a measure of the distribution of molecular mass in a given polymer sample. (wikipedia.org)
  • M n {\displaystyle M_{\mathrm {n} }} is more sensitive to molecules of low molecular mass, while M w {\displaystyle M_{\mathrm {w} }} is more sensitive to molecules of high molecular mass. (wikipedia.org)
  • Starting from 1984, first in Frankfurt and later in Münster, the development of the MALDI technique took place. (uni-frankfurt.de)
  • A sample of objects that have an inconsistent size, shape and mass distribution is called non-uniform. (wikipedia.org)
  • When these fragments are separated on a suitable gel matrix the sequence information can be obtained from the migration order of the bands (bottom to top). (jenabioscience.com)
  • A collection of objects is called uniform if the objects have the same size, shape, or mass. (wikipedia.org)
  • In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions. (cdc.gov)
  • In this study, we compared tandem mass spectrometry (MS/MS) on matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) and nano-electrospray ionization (nESI) Orbitrap instruments with respect to their ability to identify phosphopeptides from complex proteome digests. (nih.gov)
  • 11. Global and site-specific detection of human integrin alpha 5 beta 1 glycosylation using tandem mass spectrometry and the StrOligo algorithm. (nih.gov)
  • 12. Structural characterization of N-linked oligosaccharides on monoclonal antibody cetuximab by the combination of orthogonal matrix-assisted laser desorption/ionization hybrid quadrupole-quadrupole time-of-flight tandem mass spectrometry and sequential enzymatic digestion. (nih.gov)
  • Identifying the exact structures of these microheterogenious lipid As, obtained from Gram-negative bacteria grown in presence of phages, were by using mass spectrometry and tandem mass spectrometry techniques using different MSMS instruments, allowed to conclude that the biosynthesis of these series of mutant rough LPSs were indeed affected and produced a heterogeneous mixtures of different lipid As. (mun.ca)
  • The use of liquid chromatography-tandem mass spectrometry (LC-MS) or matrix-assisted laser desorption-ionization (MALDI) has been reported in the case of monitoring the removal of Tween 20 and BRIJ-35 (8). (spectroscopyonline.com)
  • 11. Brandhorst G, Oellerich M, Maine G, Taylor P, Veen G, Wallemacq P. Liquid chromatography-tandem mass spectrometry or automated immunoassays: what are the future trends in therapeutic drug monitoring? (asianjpr.com)
  • Orthogonal Analysis - V-Tag labeled glycopeptides can be analysed by MALDI-MS and (U)HPLC to give you reliable glycan identification and quantitation. (ludger.com)
  • Nano HPLC is a relatively new development in chromatography world driven by recent advancements in proteomics that would require decreasing of inner diameter (ID) of liquid chromatography (LC) column to allow for a smaller sample amount and to increase sensitivity. (justchromatography.com)
  • In order to understand taste components in the stewed chicken broth of Beijing Youji, the compositions of free amino acids and nucleotides were analyzed by high performance liquid chromatography (HPLC) and the taste active values (TAVs) of the nucleotide compounds were calculated. (spkx.net.cn)
  • chlorophenyl)-3,6-dichloro-1,4-benzoquinone, were incubated with cytochrome c, and adducts were detected by liquid chromatography-mass spectrometry (LC-MS) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI TOF). (nih.gov)
  • Twenty protein spots were randomly selected, digested with trypsin, and analyzed using Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) or liquid chromatography mass spectrometry (LC-MS/MS). While these results suggest that phenol method and the direct lysis method are more efficient and reliable for the 2D separation of Heterodera glycines proteins, the phenol extraction procedure is superior for the alkaline proteins. (usda.gov)
  • Matrix-Assisted Laser Desorption/Ionisation (MALDI) is a mass spectrometry technique that uses a laser to create ions from sample. (anthias.co.uk)
  • Individual spots from 2D gels were trypsin digested and the tryptic fragments were analyzed by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. (nih.gov)
  • 10. Molecular mass determination of plasma-derived glycoproteins by ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with internal calibration. (nih.gov)
  • 13. Ionisation and fragmentation of complex glycans with a quadrupole time-of-flight mass spectrometer fitted with a matrix-assisted laser desorption/ionisation ion source. (nih.gov)
  • 19. Sequencing of N-linked oligosaccharides directly from protein gels: in-gel deglycosylation followed by matrix-assisted laser desorption/ionization mass spectrometry and normal-phase high-performance liquid chromatography. (nih.gov)
  • Then this fraction was subjected to matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) analysis, and peaks with higher intensity were selected for tandem MS analysis. (spkx.net.cn)
  • Metabolite localization by atmospheric pressure high-resolution scanning microprobe matrix-assisted laser desorption/ionization mass spectrometry imaging in whole-body sections and individual organs of the rove beetle Paederus riparius. (uni-bayreuth.de)
  • Correlative mass spectrometry imaging, applying time-of-flight secondary ion mass spectrometry and atmospheric pressure matrix-assisted laser desorption/ionization to a single tissue section. (uni-bayreuth.de)
  • After a wash step, proteins and peptides of selected affinity are retained on the chip and analyzed by mass spectrometry time-of-flight technology similar to MALDI (matrix-assisted laser desorption and ionization). (nih.gov)
  • Gel permeation chromatography showed that, depending on the nature of p-quinone, macroinitiator polymers exhibit different activity in post-polymerization. (lookchem.com)
  • 20. Using mass spectrometry to explore the neglected glycan moieties of the antiophidic proteins DM43 and DM64. (nih.gov)
  • Following Tax immunoprecipitation from the 1,800 kDa fraction, washes with high salt buffer, 2D gel separation, and MALDI-TOF mass spectrometry analysis, a total of 32 proteins were identified. (nih.gov)
  • Twenty of whole proteins of Xinqiao recognized by the infection sera were identified by mass spectrometry. (biomedcentral.com)
  • As with peptides and proteins, oligos are desalted prior to analysis, all buffers and salts must be removed for mass spec analysis as it suppresses ionization and many buffers are observed as background in the mass spectrum. (ohio-state.edu)
  • Proteins are recorded as mass signature 'peaks' and displayed as a standard chromatograph. (nih.gov)
  • Furthermore, SELDI provides a complementary approach to 2D chromatography because SELDI is able to profile proteins regardless of their intrinsic hydrophobicity and has its best sensitivity (in the attomole range) to proteins below 15 kDa a problematic size range for 2D-PAGE resolution. (nih.gov)
  • SimulTOF Systems is engaged in multiple initiatives aimed at developing products that will enable transition from peptide and protein separations to MALDI TOF (TOF/TOF) mass spectrometry. (simultof.com)
  • Monolithic column technology was utilized in the construction of 3-dimensional MALDI plates for interfacing high flow, high capacity LC separations with MALDI. (simultof.com)
  • The 3-D plate design effectively captures analyte of interest on a porous polymer surface while allowing carrier solvents to pass through.SimulTOF Systems is engaged is multiple initiatives aimed at developing products that will enable the transition from peptide and protein separations to MALDI TOF (TOF/TOF) mass spectrometry. (simultof.com)
  • Glass µchannel plates (25µm), as well as plates constructed around reticulated vitreous carbon foam, have been utilized for interfacing electrophoretic separations with MALDI. (simultof.com)
  • The facility is equipped with other peripheral devices that allow the homogenization and sonication of samples and lyophilization and fractionation of peptides before liquid chromatography-mass spectrometry separations. (edu.pl)
  • The second step is the enrichment and clean-up of the labeled glycopeptides using a hydrophilic interaction liquid chromatography (HILIC) cartridge. (ludger.com)
  • Detailed protocols involving protein microarrays, liquid chromatography, high throughput cloning of expression constructs, IMAC, mass spectrometry, MALDI TOF, and MudPIT are provided, along with well illustrated descriptions of experimental procedures and lists of recommended Web sites and reading material. (cshlpress.com)
  • High performance gel liquid chromatography analysis showed that the fraction was mainly composed of oligopeptides (accounting for 84.29% of the total peak area) with molecular mass (spkx.net.cn)
  • Liquid chromatography mass spectrometry is a powerful technique which is used for the new product development, manufacturing, and to the control the stability or drug abuse. (asianjpr.com)
  • So, this review aims to highlight the basic introduction, and principle involved in liquid chromatography-mass spectrometry (LC-MS). Also, the advantages, or application of LC-MS were studied. (asianjpr.com)
  • 1. Arpino P. Combined liquid chromatography mass spectrometry. (asianjpr.com)
  • Principles and applications of liquid chromatography-mass spectrometry in clinical biochemistry. (asianjpr.com)
  • 12. Metz TO, Zhang Q, Page JS, Shen Y, Callister SJ, Jacobs JM, Smith RD. Future of liquid chromatography-mass spectrometry in metabolic profiling and metabolomic studies for biomarker discovery. (asianjpr.com)
  • Method development and validation for the determination of selected endocrine disrupting compounds by liquid chromatography mass spectrometry and isotope pattern deconvolution in water samples. (ac.ir)
  • These MS and MS/MS complex structural investigation were accomplished via the use of different hybrid MS instruments such as: ESI-MS with a fourier transform ion cyclotron (FTICR), a triple quadrupole (QqQ), and MALDI-TOF/TOF-MS instruments. (mun.ca)
  • 5. Facile MALDI-MS analysis of neutral glycans in NaOH-doped matrixes: microwave-assisted deglycosylation and one-step purification with diamond nanoparticles. (nih.gov)
  • Hydrolysis of the different LPS was accomplished with 1% acetic acid, and purification was by chromatography (Gel filteration affinity) using Sephadex G-50 and SephadexG-15. (mun.ca)
  • Purification of bovine striatal dopamine D-2 receptor by affinity chromatography. (mcmaster.ca)
  • Imaging mass spectrometry was employed to characterize the spatial distribution of TAF and its principal five metabolites in local tissues surrounding the implant. (nature.com)
  • Metabolomic and pharmacokinetic studies are performed by the Laboratory of Quantitative Analysis of Drugs and Metabolites (registration no. 3089, National Chamber of Laboratory Diagnosticians), which is part of the Mass Spectrometry Facility. (edu.pl)
  • A comprehensive high-resolution mass spectrometry approach for characterization of metabolites by combination of ambient ionization, chromatography and imaging methods. (uni-bayreuth.de)
  • The most commonly used detergent removal methods include size-based exclusion (dialysis and gel filtration), hydrophobic adsorption, and ion-exchange chromatography (10). (spectroscopyonline.com)
  • The reinitiation mechanism was studied by mass spectrometry (MALDI-TOF) and ESR spectroscopy. (lookchem.com)
  • Spectroscopy columnist Ken Busch once again brings readers his comprehensive list of common acronyms used in the field of mass spectrometry. (spectroscopyonline.com)
  • However, detergents can interfere with applications such as enzyme-linked immunosorbent assays, isoelectric focusing, nuclear magnetic resonance spectroscopy, and mass spectrometry, and therefore, often need to be removed before analysis. (spectroscopyonline.com)
  • In that respect, low- and high-energy CID-MS/MS (known as tandem-in-space) and FTICR-MS/MS (known as tandem-in-time) mass spectrometry analyses were chosen for iii the structural elucidation of this different series of lipid As isolated from A. liquefaciens SJ-19, hydrophilla SJ-55, and salmonicidia SJ-113a. (mun.ca)
  • Our facility performs biologically and medically oriented mass spectrometry analyses. (edu.pl)
  • Surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF) is a promising technique for rapid protein-pattern analysis of serum and microdissected tissue specimens. (nih.gov)
  • Polymer distribution of polar and non-polar polymers can be determined using MALDI-TOF MS. Polymers must be soluble in water or organic solvent for analysis. (ohio-state.edu)
  • Proteomic services include protein mass determination, protein identification from gel or solution, qualitative and semi-quantitative characteristics of the protein interactome, and the characterization and mapping of a wide spectrum of posttranslational modifications. (edu.pl)
  • The mass spectrometry-based methods of therapeutic drug monitoring generally have better quantitative precision than immunoassays at a comparable cost. (edu.pl)
  • The Mass Spectrometry Facility is engaged in a wide array of proteomic, metabolomic, and structural studies. (edu.pl)
  • 14. Analysis of the glycosylation pattern of plant copper amine oxidases by MALDI-TOF/TOF MS coupled to a manual chromatographic separation of glycans and glycopeptides. (nih.gov)
  • In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions. (cdc.gov)
  • 6. Characterization of N-glycans of recombinant human thyrotropin using mass spectrometry. (nih.gov)
  • 16. A rapid mass spectrometric strategy for the characterization of N- and O-glycan chains in the diagnosis of defects in glycan biosynthesis. (nih.gov)
  • Consequently, mass spectrometry has emerged as a reliable and sensitive method for the characterization of protein phosphorylation sites [ 7 ] and may therefore represent a method of choice for the analysis of protein phosphorylation [ 8 ]. (biomedcentral.com)
  • Mass spectrometric approaches to protein structure characterization are offered, including hydrogen-deuterium exchange, X-linking, native mass spectrometry, and ion mobility. (edu.pl)
  • MALDI-TOF is one of the most versatile mass spectrometry techniques and can be applied for the characterisation of almost any class of compounds. (anthias.co.uk)
  • Mass spectrometry analysis showed that a C-terminal peptide (Val 327 - Asn 356 ) was present in Nit-ANigRec but missing in Nit-ANigWT and Asp 298 -Val 313 peptide was shortened to Asp 298 -Arg 310 in Nit-ANigWT. (biomedcentral.com)
  • Sample handling for mass spectrometric proteomic investigations of human sera. (genomeweb.com)
  • We specialize in glycosylation analysis using chromatographic and mass spectrometric techniques and a combination thereof. (ludger.com)
  • N-glycosylation profiles of the SARS-CoV-2 spike D614G mutant and its ancestral protein characterized by advanced mass spectrometry. (cdc.gov)
  • 2. Analysis of protein glycosylation by mass spectrometry. (nih.gov)
  • Dr Radoslaw Kozak , Head of Glycan Analysis Services, will be attending BioProcess International Conference & Exhibition on September 19th-21st 2023 in Boston , Massachusetts, USA. (ludger.com)
  • Due to the pulsed nature of the laser source, it is usually combined with a time-of-flight (TOF) analyser, in order to separate ions of differing mass-to-charge ratio. (anthias.co.uk)
  • Histology-guided high-resolution AP-SMALDI mass spectrometry imaging of wheat-Fusarium graminearum interaction at the root-shoot junction. (uni-bayreuth.de)
  • High resolution mass spectrometry imaging of plant tissues : towards a plant metabolite atlas. (uni-bayreuth.de)
  • Analysis of cyathane-type diterpenoids from Cyathus striatus and Hericium erinaceus by high-resolution MALDI MS imaging. (uni-bayreuth.de)
  • Protein spots obtained from the phenol method were subjected to further analysis to test their suitability for identification by mass spectrometry. (usda.gov)
  • M n {\displaystyle M_{\mathrm {n} }} is more sensitive to molecules of low molecular mass, while M w {\displaystyle M_{\mathrm {w} }} is more sensitive to molecules of high molecular mass. (wikipedia.org)
  • and MALDI-TOF-MS/MS (high-energy CID-MS/MS) of a heterogeneous mixture of lipid As. (mun.ca)
  • The concomitant uses of high-energy CID-MS/MS obtained with the MALDI-TOF/TOF-MS instrument allowed the further elucidation of these complex biomolecules and can be effectively used for any structural identifaction studies. (mun.ca)
  • High-resolution mass spectrometry driven discovery of peptidic danger signals in insect immunity. (uni-bayreuth.de)
  • 6. Vas G, Vekey K. Solid‐phase microextraction: a powerful sample preparation tool prior to mass spectrometric analysis. (ac.ir)
  • Free from the time constraints inherent in "on-line" LCMS techniques, LC-MALDI protocols allow for LC runs to be scanned in MS mode and the resulting parent-mass information can then be used in a true data-dependent manner to set up the MS-MS measurements. (simultof.com)
  • However, many methods developed in synthetic polymer systems cannot be readily applied to protein hydrogels, and manipulating the mechanical response of the gels presents a new challenge. (frontiersin.org)
  • UV absorbance, an array of colorimetric methods, and mass spectrometry (MS) are current options to monitor and optimize the efficiency of detergent removal (8,11,12). (spectroscopyonline.com)
  • Novel Mass Spectrometric Methods for Evaluation of Plasma Angiotensin Converting Enzyme 1 and Renin Activity. (genomeweb.com)
  • This study presents the mass spectrometry structural elucidations of the extracted heterogeneous lipid As mixtures, obtained from native lipopolysaccharides (LPSs) isolated from different types of the Vibrionaceae family. (mun.ca)
  • 2011. Sequence-Specific Capture of Protein-DNA Complexes for Mass Spectrometric Protein Identification . (wisc.edu)
  • Because the function of a protein is related to its hierarchical structure and the diversity of amino acid functional groups, site-specific and orthogonal reactions are often required to preserve protein properties in the final gel. (frontiersin.org)
  • According to our mass spectrometry and circular dichroism results, quercetin was able to promote a chemical modification of some amino acid residues and modify the secondary structure of C. d. terrificus sPLA 2 . (hindawi.com)
  • Removal of mPEG-OH requires the use of preparative ion exchange column chromatography, which is time consuming and expensive. (allindianpatents.com)
  • Column chromatography: A method of separating and purifying substances using a cylindrical container (column). (hokudai.ac.jp)