• Kinetic properties and thermal stabilities of Hoplias malabaricus liver and skeletal muscle unfractionated malate dehydrogenase (MDH, EC 1.1.1.37) and its isolated isoforms were analyzed to further study the possible sMDH-A* locus duplication evolved from a recent tandem duplication. (scielo.br)
  • Malate dehydrogenase (EC1.1.1.37) is an enzyme in the citric acid cycle that catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). (prospecbio.com)
  • A thermostable l-malate dehydrogenase from the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus was isolated and characterized, and its gene was cloned and sequenced. (nih.gov)
  • Crystal structure of the MJ0490 gene product of the hyperthermophilic archaebacterium Methanococcus jannaschii, a novel member of the lactate/malate family of dehydrogenases. (semanticscholar.org)
  • Isolation, nucleotide sequence analysis, and disruption of the MDH2 gene from Saccharomyces cerevisiae: evidence for three isozymes of yeast malate dehydrogenase. (ymdb.ca)
  • An example is the gene that encodes an enzyme called lactate dehydrogenase (LDH). (elifesciences.org)
  • Four progeny rows, from experiments designed to test for recombination or allelism at the k2 (tan saddle) locus, y20 (yellow foliage) locus, or the Mdh1-n (mitochondrial malate dehydrogenase one null) locus, had both fertile and sterile plants (Chen and Palmer, 1996b, 1998b). (thefreedictionary.com)
  • Lyophilized Malate dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution MDH1 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles. (mybiosource.com)
  • The DNA encoding Malate (Malic) Dehydrogenase is cloned from cDNA library of chicken heart.The MDH1 is purified by proprietary chromatographic techniques. (mybiosource.com)
  • Crowding increases Km of mitochondrial MDH (show MDH1 ELISA Kits ) for malate oxidation. (antibodies-online.com)
  • Since wild-type Saccharomyces cerevisiae strains produce only low levels of malate, metabolic engineering is required to achieve efficient malate production with this yeast. (asm.org)
  • Metabolic flux analysis showed that metabolite labeling patterns observed upon nuclear magnetic resonance analyses of cultures grown on 13 C-labeled glucose were consistent with the envisaged nonoxidative, fermentative pathway for malate production. (asm.org)
  • Data indicate that acetyl-CoA (show LPCAT2 ELISA Kits ) acetyltransferase ( ACAT1 (show ACAT1 ELISA Kits )) and malate dehydrogenase (MDH2 (show MDH ELISA Kits )) are involved in various drug-resistance-forming mechanisms. (antibodies-online.com)
  • Bands were revealed for the enzyme systems esterase (EST), according to Scandalios (1969), alcohol dehydrogenase (ADH), phosphoglucose isomerase (PGI), malate dehydrogenase (MDH) and malic enzyme (ME), described by Alfenas (1991), and alpha amylase ([alpha] - AMY), according to Alfenas (1998). (thefreedictionary.com)
  • Malate that is then generated from αKG can exit the TCA cycle and be converted to pyruvate by malic enzyme (ME), which contributes to redox homeostasis via NADPH production. (jci.org)
  • Structural basis for light activation of a chloroplast enzyme: the structure of sorghum NADP-malate dehydrogenase in its oxidized form. (ebi.ac.uk)
  • Chloroplast NADP-malate dehydrogenase: structural basis of light-dependent regulation of activity by thiol oxidation and reduction. (ebi.ac.uk)
  • K-m for malate was 100 mu M and 458 mu M in the presence of NAD(+) and NADP(+), respectively. (uclouvain.be)
  • K-m for NAD(+) and for NADP(+) in the presence of malate was 18 mu M and 207 mu M, respectively. (uclouvain.be)
  • The kinetic data do not contradict the consecutive mechanism of the reaction with the ordered addition of substrates: NADP binds first, then malate. (meta.org)
  • The enzyme conformation initiated by NADP and malate binding is less thermostable. (meta.org)
  • The increasing affinity of MDH for malate and the decreasing MDH activity and expression in the forward reaction may be an acceptable molecular target for eliminating its possible impact on tumorigenesis. (alliedacademies.org)
  • Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. (prospecbio.com)
  • A promising pathway for malate production from glucose proceeds via carboxylation of pyruvate, followed by reduction of oxaloacetate to malate. (asm.org)
  • This redox- and ATP-neutral, CO 2 -fixing pathway has a theoretical maximum yield of 2 mol malate (mol glucose) −1 . (asm.org)
  • In glucose-grown batch cultures, the resulting engineered strain produced malate at titers of up to 59 g liter −1 at a malate yield of 0.42 mol (mol glucose) −1 . (asm.org)
  • Isozyme analysis: In this study, polymorphism among parental lines of two F1 hybrids Shaktiman-1 [(CML 142 x CML 150) x CMLand Shaktiman-2 (CML 176 x CML186) with respect to the esterase (EST), alcohol dehydrogenase (ADH), malate dehydrogenase (MDH) and acid phosphatase (ACP) was analyzed. (thefreedictionary.com)
  • hypotetical aryl-alcohol dehydrogenase. (bio.net)
  • This ability is unique because NADPH is used as the reductant in biosynthetic reactions, while NAD+ is usually used as the oxidant in most catabolic reaction, and glutamine dehydrogenase is not specific to either. (wikibooks.org)
  • Malate Dehydrogenase (MDH), as a critical enzyme in cancer metabolism, can be the main foundation for NAD, along with the well-known LDH, which allows the prolonged process of glycolysis, even in the absence of oxygen. (alliedacademies.org)
  • Malate dehydrogenase (MDH) may be important in carbohydrate and energy metabolism in malarial parasites. (elsevier.com)
  • Use Malate Dehydrogenase in diagnostic tests for the determination of aspartate aminotransferase or in applications for citric and acetic acid testing. (roche.com)
  • S )-Sulfolactate is an intermediate in the biosynthesis of coenzyme M, and ( S )-malate is an intermediate in the partial citric acid cycle. (asm.org)