Major capsid proteins. Medical search. Frequent questions

The VP1 protein, along with capsid components VP2 and VP3, is expressed from the "late region" of the circular viral genome. (wikipedia.org)
All of the capsid proteins are expressed from the late region of the viral genome, so named because expression occurs only late in the infection process. (wikipedia.org)
The extra sequences at the N-termini of viral jelly roll capsid proteins, involved in recognizing the viral genome, likely evolved after the capture of these proteins from cells. (virology.ws)
One isolate, PBCV-1, for example, (genome size = 330744 bp dsDNA) codes for ~375 proteins, nearly half of which resemble proteins of known function. (uml.edu)
Working with partners at the Pasteur Institute and French National Institute for Agricultural Research (Institut National de la Recherche Agronomique - INRA) we recently published an atomic resolution model of the RSV nucleocapsid - a helical structure comprising the viral genome and N protein. (gla.ac.uk)
We have published structures of FCV decorated with soluble fragments of JAM-A, revealing conformational changes in the capsid that we propose to be the first stages of uncoating (genome release). (gla.ac.uk)
Adenovirus major core protein condenses DNA in clusters and bundles, modulating genome release and capsid internal pressure. (csic.es)
Contrary to what is observed in other isolated viruses of amoeba, Yaravirus is not represented by a large/giant particle and a complex genome, but at the same time carries an important number of previously undescribed genes, including one encoding a novel major capsid protein. (bgr.com)
Here, we reported the first Oceanospirillum phage, vB_OliS_GJ44, which was assembled into a 33,786 bp linear dsDNA genome, which includes abundant tail-related and recombinant proteins. (biomedcentral.com)
The arteriviruses are highly species specific, but share many biological and molecular properties, including virion morphology, a unique set of structural proteins, genome organization and replication strategy, and the ability to establish prolonged or true persistent infection in their natural hosts. (bionity.com)
Two major open reading frames (ORF), ORF1 and ORF2, which run in opposing directions on the 1700bp genome, code for the capsid and replicase proteins respectively. (caister.com)
Today, phage genome sequencing is commonly performed for classification and characterization purposes and is based on the arrangement of conserved genes and the nucleotide and protein sequence identity [ 5 , 6 ]. (biomedcentral.com)
The genome of coliphage T7 has 56 genes encoding potential proteins [ 8 ], of which 35 have known function or functions. (biomedcentral.com)

The involvement of viral DNA-binding proteins in the regulation of virulence genes, transcription, DNA replication, and repair make them significant targets. (mdpi.com)
It seems likely that viral structural proteins originated from cellular genes. (virology.ws)
Many of these genes encode proteins (e.g., enzymes involved in protein glycosylation) rarely associated with viruses. (uml.edu)
Analysis of two highly expressed genes from Chlorella virus PBCV-1: Protein characterization and the DNA sequences of the major capsid protein gene and the early/late 33-kDa protein gene. (uml.edu)
Here, we build large, intact carboxysome shells (over 90 nm in diameter) in the industrial microorganism Escherichia coli by expressing a set of carboxysome protein-encoding genes. (nature.com)
Some genes no longer code for a protein due to a mutation or error. (rationalwiki.org)
Streamlining Regulon Identification in Bacteria Regulons are a group of genes that can be turned on or off by the same regulatory protein. (doe.gov)
ORF-2 contains genes encoding the capsid. (medscape.com)

Protein sequences were clustered by the pairwise sequence similarity using CLANS. (biomedcentral.com)
The deduced amino acid sequences of the outer capsid protein, VP7, of serotype G1 rotavirus clinical isolates collected over a 6 year period (1990-1995) in Melbourne, Australia, were examined. (microbiologyresearch.org)
Phylogenetic analysis characterized the sequences into two discrete clusters representing two of the four global lineages of human G1 VP7 proteins. (microbiologyresearch.org)
Nucleotide sequences encoding the major capsid protein VP1 were determined for each isolate. (cdc.gov)
Primers were designed to target sequences in the nodavirus capsid protein gene, yielding an amplification product of 120 nucleotides. (int-res.com)
In general, immunobiologists have developed an integrated method for vaccine development based on analyzing protein sequences and structures of target viruses [ 10 ]. (biomedcentral.com)

Anti-Norovirus (VLP major capsid protein of GI and GII) monoclonal antibody, clone C2049N - 1mg - 1 vial is backordered and will ship as soon as it is back in stock. (karlan.com)

VP1 is the major structural component of the polyomavirus icosahedral capsid, which has T=7 symmetry and a diameter of 40-45 nm. (wikipedia.org)
We have previously discussed the idea that viruses originated from selfish genetic elements such as plasmids and transposons when these nucleic acids acquired structural proteins (see A plasmid on the road to becoming a virus ). (virology.ws)
I want to explore in more detail the idea that the structural proteins of viruses likely originated from cell proteins ( link to paper ). (virology.ws)
Missing from these hypothesis is how nucleic acids became virus particles - that is, how they acquired structural proteins. (virology.ws)
The core proteins of alphaviruses (think Semliki Forest virus) has structural similarity with chymotrypsin-like serine proteases. (virology.ws)
Retroviral structural proteins also appear to have originated from cell proteins, with clear homologies with matrix, capsid, and nucleocapsid proteins. (virology.ws)
At some point these genetic elements acquired structural proteins from the cells and became bona fide virus particles. (virology.ws)
SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
Nineteen national-level public health laboratories performed routine dengue diagnostic assays on a proficiency testing panel consisting of two modules: one containing commercial serum samples spiked with cultured dengue viruses for the detection of nucleic acid and non-structural protein 1 (NS1) (Module A) and one containing human serum samples for the detection of anti-dengue virus antibodies (Module B). A review of logistics arrangements was also conducted. (who.int)
An international collaboration between the UCL School of Pharmacy, the Lund Protein Production Platform (LP3) and ESS, through its DEMAX platform, have performed biophysical and structural studies of three non-structural proteins from the novel coronavirus, SARS CoV-2, the causative agent of COVID-19. (lu.se)

CroV MCP gene was codon optimized, synthesized, and amplified by PCR followed by cloning into a bacterial expression system in order to obtain purified protein of high homogeneity. (utep.edu)
However, when recombination analyses were performed using RDP4, no recombination was found in either the partial major capsid protein (MCP) gene or the putative eukaryotic initiation factor 2 - α (eIF2-α) homologue gene. (gaacademy.org)
It also holds a DNA polymerase gene and major capsid protein gene very similar to those of the amoeba. (sciencedaily.com)
HPVs are classified by genotype, and at least 130 types have been identified by sequencing the gene encoding the major capsid protein L1. (biochain.com)
The Capsid Protein of Nervous Necrosis Virus Antagonizes Host Type I IFN Production by a Dual Strategy to Negatively Regulate Retinoic Acid-Inducible Gene-I-like Receptor Pathways. (bvsalud.org)

Genomic dsRNA remains enclosed by the inner capsid, thereby "hiding" from host antiviral mechanism. (expasy.org)
The packaging NTPase P4 translocates the three ssRNA(+) genomic segments in the capsids where they are converted into dsRNA by the viral RdRp P2. (expasy.org)

Major capsid protein VP1 is a viral protein that is the main component of the polyomavirus capsid. (wikipedia.org)
We postulate that the terminal β-hairpin locks other polyomavirus capsids in a stable conformation and that absence of the hairpin leads to the observed capsid size variation in APV. (rcsb.org)

An analysis of the sequence an structure of major virion proteins has identified likely ancestors in cellular proteins. (virology.ws)
The virion has a double capsid structure: the outer capsid has a T=13 icosahedral symmetry and the inner capsid has a T=2* icosahedral symmetry . (expasy.org)
Multimeric protein P3 adsorb to host type IV pili which retracts bringing the virion in contact with the host membrane (e.g. (expasy.org)

Chakroborty, Sayan, "Expression and characterization of major capsid protein (MCP) of a giant marine virus: Cafeteria roenbergensis virus (CroV)" (2013). (utep.edu)
Further similarities were uncovered after the discovery and characterization of a type of large DNA virus called "giant virus," which can be up to 2.5 µm in diameter and contain DNA "encoding" information for the production of more than 400 proteins. (sciencedaily.com)

WB/ELISA positive controls from Made for Virology provide stable, validated protein standards for a wide range of human viruses including respiratory tract pathogens, as well as, animal and marine pathogens. (nanoexpo.eu)
The matrix Z proteins of arenaviruses are related to cellular RING domain proteins, and the matrix proteins of some negative strand RNA viruses are related to cellular cyclophilin. (virology.ws)
There are many more examples, providing support for the hypothesis that viruses evolved on multiple instances by recruiting different cell proteins. (virology.ws)
Given this information on the origin of viral capsid proteins, we can modify the three hypotheses for the origin of viruses into one. (virology.ws)
Now, the question if of course - is it of viral origin or is it a cellular protein co-opted by viruses? (virology.ws)
Some viruses possess an outer envelope, and the outer capsid is composed of major capsid protein (MCP), which appears to be highly conserved among the family and possesses surface binding sites interacting with the surfaces of host's cells [ 2 ]. (biomedcentral.com)
Soil Virus Offers Insight into Maintaining Microorganisms Through a collaborative effort, researchers have identified a protein in soil viruses that may promote soil health. (doe.gov)
In addition, there are 25 nonessential proteins, of which only 12 are conserved across the genus "T7-like viruses" [ 8 ]. (biomedcentral.com)

A feature unique to vesiviruses is the presence of a ~14-18 kDa protein of unknown function designated as leader of the capsid (LC) that is expressed as part of a capsid precursor encoded in open reading frame 2 (ORF2). (umd.edu)
The ORF2 precursor contains the LC fused to the major capsid protein, VP1, which is proteolytically cleaved by the viral protease to release the LC and the mature VP1. (umd.edu)

The C-terminus also contains a basic nuclear localization sequence,: 316 while the N-terminus - which is oriented toward the center of the assembled capsid - contains basic residues that facilitate non-sequence-specific interactions with DNA. (wikipedia.org)
This family contains a number of major capsid Gp23 proteins approximately 500 residues long, from T4-like bacteriophages. (nih.gov)

Gardasil9 is a recombinant human papillomavirus (HPV) 9-valent vaccine, containing purified major capsid L1 protein of HPV types 6, 11, 16, 18, 31, 33, 45, 52, and 58 re-assembled into virus-like particles (VLPs) as the active ingredient. (sanevax.org)
Since the antigens are purified recombinant proteins, Gardasil9 needs a potent adjuvant to enhance the initiation of the immune response through activation of innate immunity of the host to generate high and sustained levels of antibodies for maintaining efficacy of vaccination. (sanevax.org)
Immunoprecipitation of recombinant LC in FCV infected cells identified the cellular protein annexin A2 as a binding partner, providing a potential mechanism for the cell-rounding phenotype observed in transient expression experiments. (umd.edu)
Recombinant DNA technology is used to prepare HPV vaccines from the major capsid (L1) protein of HPV. (msdmanuals.com)

FIG 1 Timeline of major events in the history of research on phages, phage therapy, and antibiotics. (asm.org)
Twenty eight (46.7%) of the proteins encoded by this virus exhibit sequence similarity to coliphage T7 proteins while 34 (81.0%) are similar to proteins of Pseudomonas phage gh-1. (biomedcentral.com)
Villanueva Valencia J. R., Tsimtsirakis E., Krueger S., Evilevitch A., "Temperature-induced DNA density transition in phage λ capsid revealed with contrast-matching SANS. (lu.se)

The VP1 protein monomer is primarily composed of beta sheets folded into a jelly roll fold. (wikipedia.org)
A very common motif among viral capsid proteins is called the single jelly roll , made up of eight beta strands in two four-stranded sheets. (virology.ws)

This property of CW3 is dependent on its major capsid protein, a virulence determinant. (broadinstitute.org)

The viral core protein retains protease activity, needed for cleavage from a protein precursor. (virology.ws)

These bacteriophages appear to be most similar to bacteriophages that infect Pseudomonas and Ralstonia rather than Enterobacteriales bacteria by protein similarity, however, we were only able to detect infection of Erwinia and the closely related strains of Pantoea . (frontiersin.org)

The carboxysome is a specialized bacterial organelle that encapsulates enzymes into a virus-like protein shell and plays essential roles in photosynthetic carbon fixation. (nature.com)
The naturally designed architecture, semi-permeability, and catalytic improvement of carboxysomes have inspired rational design and engineering of new nanomaterials to incorporate desired enzymes into the protein shell for enhanced catalytic performance. (nature.com)

Each segments encode for a polycystronic mRNA, translated into several proteins by the procaryotic translation machinery. (expasy.org)

VP2 and VP3 assemble inside the capsid in contact with VP1, with a stoichiometry of one VP2 or VP3 molecule to each pentamer. (wikipedia.org)
Neo-synthesized proteins assemble into new capsids. (expasy.org)
The L1 proteins self-assemble into noninfectious, nononcogenic virus-like particles (VLPs). (msdmanuals.com)

By modeling the interaction at atomic resolution we determined a list of key amino-acids on the capsid surface that mediate the interaction. (gla.ac.uk)

oligomerization to form capsids occurs in the nucleus. (wikipedia.org)
This process occurs concomitantly with the inhibition of cellular protein synthesis, caused by the expression of viral proteases. (bionity.com)
A specific group of phages has a number of conserved proteins but also a relatively high number of proteins for which no match occurs in the current databases. (biomedcentral.com)

Their proteomes are highly unstudied, with over 200 putative proteins with no known homologs. (frontiersin.org)
The production of 27 of these putative proteins was confirmed by mass spectrometry analysis. (frontiersin.org)

Histones are proteins that keep DNA strands curled up and packed into the cell nucleus. (sciencedaily.com)
Cell macromolecular synthesis may be controlled after CoV infection by locating some virus proteins in the host cell nucleus. (bionity.com)
Evilevitch A. Hohlbauch S.V. ''Intranuclear, HSV-1 DNA ejection induces major mechanical transformations suggesting mechanoprotection of nucleus integrity. (lu.se)

VP1 is the primary component and forms a 360-unit outer capsid layer composed of 72 pentamers. (wikipedia.org)
The VP1 C-terminus is disordered and forms interactions between neighboring pentamers in the assembled capsid. (wikipedia.org)
Plug-like density features were observed at the base of the VP1 pentamers, consistent with the known location of minor capsid proteins VP2 and VP3. (rcsb.org)

The largest, ORF-1, codes for the nonstructural proteins responsible for viral replication. (medscape.com)

CoV N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. (bionity.com)

Interferon-Induced Protein 44 Interacts with Cellular FK506-Binding Protein 5, Negatively Regulates Host Antiviral Responses, and Supports Virus Replication. (csic.es)
Both viral and cellular proteins are required for replication and transcription. (bionity.com)
In this study, we reported that capsid protein (CP) of red-spotted grouper NNV (RGNNV) suppressed the IFN antiviral response to promote RGNNV replication in Lateolabrax japonicus brain cells , which depended on the ARM , S, and P domains of CP. (bvsalud.org)

CroV is covered by a protein shell (capsid) comprised of major capsid proteins (MCP) and minor capsid proteins (mCP). (utep.edu)
However, the plug density is more prominent in APV and may include VP4, a minor capsid protein unique to bird polyomaviruses. (rcsb.org)

The capsid not only protects the genetic material of the virus, but also helps in host-virus recognition, which is the critical initial step for viral infection. (utep.edu)

Evilevitch A, Sae-Ueng U., ''Mechanical Capsid Maturation Facilitates the Resolution of Conflicting Requirements for Herpesvirus Assembly. (lu.se)

In the spring of 2020, they managed to solve and started to analyse one of these proteins, Nsp10, by using the BioMAX beamline at MAX IV Laboratory. (lu.se)

They contain a 'RGD' sequence, identical to the recognition site of many adhesion proteins. (embl.de)

The virus structure contains three domains, including an outer proteinaceous capsid, an intermediate lipid membrane, and a central core. (biomedcentral.com)
Protein P6 mediates fusion of virus membrane with host outer membrane, releasing the capsid into the periplasmic space. (expasy.org)
The capsid enters into the cytoplasm possibly via permeabilization of the host plasma membrane. (expasy.org)

TF facilitates the proper folding of proteins at low temperature in bacteria. (utep.edu)

Many cell proteins have jelly role motifs, and some form 60-subunit virus-like particles in cells. (virology.ws)
Nervous necrosis virus (NNV), a highly pathogenic RNA virus , is a major pathogen in the global aquaculture industry . (bvsalud.org)
Nous avons déterminé la fréquence des différents sérotypes du virus de la dengue pour mettre en évidence son hyperendémicité à Rawalpindi (Pakistan). (who.int)
Les échantillons de sérum de 140 patients susceptibles d'avoir contracté le virus de la dengue ont été analysés entre mai et octobre 2015 à l'aide du test ELISA et de l'amplification en chaîne par polymérase multiplexe. (who.int)

Each WB/ELISA positive control contains purified viral protein that can serve as a binding target or an internal control for antibody validation or any antibody based assay such as western blotting or ELISA. (nanoexpo.eu)

In other species, the attachment apparatus is a heteromeric protein assembly that utilizes the rough lipopolysaccharide as a receptor (e.g. (expasy.org)
Disintegrin-like proteins are found in various species ranging from slime mold to humans. (embl.de)

FCV is the only calicivirus for which a protein cellular receptor has been identified - junctional adhesion molecule A (JAM-A). (gla.ac.uk)

One example is syncytin , a retroviral protein used for the construction of the mammalian placenta. (virology.ws)
Mammalian cells proteolytically release (shed) the extracellular domains of many cell-surface proteins. (embl.de)

After large-scale expression in bacteria, ultra-high purity TF-MCP fusion protein was obtained by affinity chromatography and size exclusion chromatography. (utep.edu)

Mass spectrum (MS) and dynamic light scattering (DLS) were performed to analyze the molecular weight and hydrodynamic diameter of the fusion protein. (utep.edu)

Processing of the viral polyprotein is achieved cotranslationally by viral encoded proteases , giving rise to the different mature viral proteins. (bionity.com)

Villanueva-Valencia,J.R., Tsimtsirakis, E., Evilevitch, A. ''Role of HSV-1 Capsid Vertex-Specific Component (CVSC) and Viral Terminal DNA in Capsid Docking at the Nuclear Pore. (lu.se)

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