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  • actin
  • The cells were also stained with SYTOX Orange and Alexa Fluor 350 conjugated to phalloidin, targeting DNA and the cytoskeletal filamentous actin network, respectively. (fsu.edu)
  • The culture of Madin-Darby canine kidney cells presented in the digital image above was labeled with DAPI and Alexa Fluor 568 conjugated to phalloidin, which target DNA in the cell nucleus and the F-actin cytoskeletal network, respectively. (fsu.edu)
  • influenza
  • The potential use of cell culture techniques in developing viral vaccines, especially for the Influenza virus, has been widely investigated in recent years as a complementary and alternative platform to the current egg-based strategies. (wikipedia.org)
  • Audsley JM, Tannock GA. Cell-based influenza vaccines: progress to date.Drugs. (wikipedia.org)
  • placenta
  • Additionally, two major transcripts of 5.5 kb and 6.5 kb have been identified in brain, kidney, liver, small intestine, placenta and lung, while only one PLEKHA7 mRNA transcript of 5.5 kb is identified in heart, brain, colon and skeletal muscle. (wikipedia.org)
  • tight junctions
  • This important function requires the presence of sealing junctions, termed tight junctions in vertebrates, which effectively bind adjacent cells together into a selective barricade. (fsu.edu)
  • When visualized in the microscope, tight junctions appear to be composed of an interconnected network of sealing strands that completely encircle the apical portion of each cell in a sheet of epithelia. (fsu.edu)
  • culture
  • It is one of few cell culture models that is suited for 3D cell culture and multicellular rearrangements known as branching morphogenesis. (wikipedia.org)
  • Over the same period in the 1980s, biologists studying cell motility had hit upon an interesting and reproducible behavior of cells in culture: the scattering response. (wikipedia.org)
  • Epithelial cells in culture grow normally as tight clusters. (wikipedia.org)
  • Beyond that immediate paradox, a crucial connection was forged between the acute induction of cell motility in 2D culture by the "scatter factor", and its impact on the spatial organization adopted by tissues in 3D. (wikipedia.org)
  • The culture of Madin-Darby canine kidney epithelial cells appearing in the digital image above was transfected with a pEYFP-Mitochondria chimeric plasmid subcellular localization vector. (fsu.edu)
  • Kizivat T, Smolić M, Marić I, Tolušić Levak M, Smolić R, Bilić Čurčić I, Kuna L, Mihaljević I, Včev A, Tucak-Zorić S. Antioxidant Pre-Treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis. (mdpi.com)
  • The Cell Culture Dish. (wikipedia.org)
  • immunofluorescence
  • After the immunofluorescence reactions, the cells were counterstained with Hoechst 33342 to reveal the location of the nuclei. (fsu.edu)
  • The generalized procedure for immunofluorescence labeling of adherent cell cultures involves fixation of the cells with a suitable reagent, such as paraformaldehyde, glutaraldehyde, acetone, or methanol. (fsu.edu)
  • fibroblasts
  • However, they could be induced to break cell-cell contacts and become elongated and motile after exposure to a "scatter factor" that was secreted by mesenchymal cells such as Swiss 3T3 fibroblasts. (wikipedia.org)
  • viral
  • Indeed, they chose to isolate kidney tubules with precisely this goal in mind, as they had previously succeeded with viral infection of cells derived from kidney tubules from other mammals. (wikipedia.org)
  • After in vitro transcription of virus-like vRNA by T3 or T7 RNA polymerase and reconstitution of viral ribonucleoprotein (vRNP) molecules, genetically altered RNP segments were introduced into eukaryotic cells by transfection. (pnas.org)
  • Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. (wikipedia.org)
  • survival
  • In all three cell lines, pre-treatment of antioxidants increased cell survival. (mdpi.com)
  • Early studies with deletions of RAB11 homologs in Saccharomyces cerevisiae proved their importance in cell survival. (wikipedia.org)