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  • kinetochore
  • In addition, HDACI-treated mitotic cells displayed SAC inactivation characteristics, including anaphase-promoting complex/cyclosome target degradation, cyclin-dependent kinase 1 inactivation, histone H3 dephosphorylation, and loss of the SAC component MAD2 from the kinetochore. (aacrjournals.org)
  • In this paper we describe the molecular evolution of one centromere/kinetochore component, originally identified as the protein product of the Drosophila melanogaster gene l(1)zw10 , hereafter called DmZW10. (rupress.org)
  • antibody
  • Mis-aligned and lagging chromosomes were induced after injection of an anti-Mad2 antibody or a Mad2 morpholino. (ehu.es)
  • MAD2L1
  • Antigen standard for MAD2L1 binding protein (MAD2L1BP), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. (creativebiomart.net)
  • Humans
  • We have identified ZW10-related proteins from widely diverse species with divergent centromere structures, including several Drosophilids, Caenorhabditis elegans , Arabidopsis thaliana, Mus musculus , and humans. (rupress.org)
  • The present data show that a Mad2 protein, highly homologous to that of humans, is expressed in this species. (ehu.es)
  • anaphase
  • This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. (cancer-genetics.org)
  • Together, these results suggest that by targeting checkpoint proteins to kinetochores, Aurora B couples chromosome alignment with anaphase onset. (nih.gov)
  • The protein encoded by this gene is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. (genecards.org)
  • An extended anaphase signaling pathway for Mad2p includes microtubule organizing center proteins and multiple motor-dependent transitions. (semanticscholar.org)
  • Proteolysis of mitotic cyclins depends on a multisubunit ubiquitin-protein ligase, the anaphase promoting complex (APC). (sdbonline.org)
  • Both processes require the Cdc14 phosphatase, whose release from the nucleolus during anaphase causes dephosphorylation and thereby activation of Cdh1 and accumulation of another protein, Sic1. (sdbonline.org)
  • peptides
  • Check out links to articles that cite our custom service antibodies, peptides, and proteins in major peer-reviewed journals, organized by research category. (abgent.com)
  • Peptides and proteins are guaranteed for 3 months from date of receipt. (novusbio.com)
  • mammalian
  • The closest mammalian NimA homologue NEK2 is a core component of the human centrosome and its activity and expression peak in S and G2 phase, during which it interacts with and phosphorylates several centrosomal proteins. (eu.org)
  • antibodies
  • Antibodies against the human ZW10 protein display a cell cycle-dependent staining pattern in HeLa cells strikingly similar to that previously observed for DmZW10 in dividing Drosophila cells. (rupress.org)
  • Cdh1
  • The APC/C Cdh1 complex targets not only D box-containing proteins but also proteins exhibiting a KEN box and/or an A box (e.g., cyclin B1 and Aurora A). Plk1 itself is an APC/C Cdh1 target. (aacrjournals.org)
  • centromere
  • We have isolated and characterized a set of overlapping cDNA clones that encode the human centromere autoantigen centromere protein C (CENP-C). The identity of these clones has been established using several criteria. (semanticscholar.org)
  • progression
  • Mad2 controls meiotic progression, but its role during sea urchin development had never been investigated. (ehu.es)