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  • Detectors
  • The most common detectors used for size exclusion chromatography include online methods similar to the bench methods used above. (wikipedia.org)
  • It was in the fifties and sixties that theoretical models were developed for IC for further understanding and it was not until the seventies that continuous detectors were utilized, paving the path for the development from low-pressure to high-performance chromatography. (wikipedia.org)
  • Recombinant
  • W.S. Hancock, A. Apffel, J. Chakel, C. Souders, T.M. Timkulu, E. Pungor,Jr., and A.W. Guzzetta, Reversed-Phase Peptide Mapping of Recombinant Human Glycoproteins Using Liquid Chromatography/Electrospray Ionization-Mass Spectrometry. (elsevier.com)
  • displacement
  • J. Frenz, Trace Analysis Peptide Mapping by High-Performance Displacement ChromatographyR.W. Giese, M. Saha, S. Abdel-Baky, and K. Allam, Measuring DNA Adducts by Gas Chromatography-Electron Capture-Mass Spectrometry: Trace Organic Analysis. (elsevier.com)
  • Displacement chromatography is a chromatography technique in which a sample is placed onto the head of the column and is then displaced by a solute that is more strongly sorbed than the components of the original mixture. (wikipedia.org)
  • Displacement chromatography found a variety of applications including isolation of transuranic elements and biochemical entities. (wikipedia.org)
  • particles
  • With the help of ultra/microfiltration it is possible to remove particles, colloids and macromolecules, so that waste-water can be disinfected in this way. (wikipedia.org)
  • protein
  • Immunoprecipitation will take place during the second dimension electrophorsis and the immunoprecipitates have a characteristic bell-shape, each precipitate representing one antigen, the position of the precipitate being dependent on the amount of protein as well as the amount of specific antibody in the gel, so relative quantification can be performed. (wikipedia.org)
  • resin
  • Sealed chromatography cartridges or columns work similarly except the sample and buffer is pumped into and through the resin by an external device such as a liquid chromatographic (LC) system, also requiring collection and monitoring of several fractions. (wikipedia.org)
  • pores
  • They all share the trait that they are a three-dimensional arrangement of intertwined strands, which produces holes (or pores) through the gel matrix. (encyclopedia.com)
  • In order for the desired macromolecules to remain in the void volume, resins with very small pores sizes must be utilized. (wikipedia.org)
  • column
  • The material inside the column is agarose based, meaning that it consists of sugars that are crosslinked to form a gel-like mass. (wikipedia.org)
  • However, there are also disadvantages involved when performing ion-exchange chromatography, such as constant evolution with the technique which leads to the inconsistency from column to column. (wikipedia.org)
  • In any form of chromatography, the rate at which the solute moves down the column is a direct reflection of the percentage of time the solute spends in the mobile phase. (wikipedia.org)
  • applications
  • The boom of Ion exchange chromatography primarily began between 1935-1950 during World War II and it was through the "Manhattan project" that applications and IC were significantly extended. (wikipedia.org)
  • detectors
  • It was in the fifties and sixties that theoretical models were developed for IC for further understanding and it was not until the seventies that continuous detectors were utilized, paving the path for the development from low-pressure to high-performance chromatography. (wikipedia.org)
  • synthetic
  • Other synthetic routes include: controlled sol-gel processes, group transfer polymerization, transition metal catalysis, living anionic polymerization, living cationic polymerization, ring opening polymerization, ring-opening metathesis polymerization (ROMP), and controlled radical polymerization. (wikipedia.org)
  • role
  • our philosophy is that by combining structural and biophysical analysis with biochemical and functional studies, we will be able to build up a complete picture of the mechanism of action of the relevant macromolecules and their role in fundamental biological processes, particularly those related to gene expression and DNA replication. (port.ac.uk)
  • They look at the role of these macromolecules in metabolic pathways, how the pathways are controlled, and the consequences of genetic or metabolic disruption. (ubc.ca)