Macromolecular complexes multiprotein complexes. Medical search. Frequent questions

Macromolecular Substances" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (uchicago.edu)
In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure. (uchicago.edu)
This graph shows the total number of publications written about "Macromolecular Substances" by people in this website by year, and whether "Macromolecular Substances" was a major or minor topic of these publications. (uchicago.edu)
Below are the most recent publications written about "Macromolecular Substances" by people in Profiles. (uchicago.edu)

CEF scientists set out to investigate the structure and function of large macromolecular complexes, in particular membrane proteins and their assemblies, complexes involved in signal transduction and quality control, and RNA-protein complexes. (wikipedia.org)
In the crowded conditions of the cell membrane, most membrane proteins associate into complex dynamic assemblies to carry out their various tasks. (wikipedia.org)
This technique allows the observation of individual kinetochore complexes and microtubule-binding proteins with single-molecule sensitivity to reveal their mode of interaction with dynamic plus-ends. (uni-due.de)
Representing species-specific proteins and protein complexes in ontologies that are both human- and machine-readable facilitates the retrieval, analysis, and interpretation of genome-scale data sets. (biomedcentral.com)
Because proteins are often functional only as members of stable protein complexes, the PRO Consortium, in collaboration with existing protein and pathway databases, has launched a new initiative to implement logical and consistent representation of protein complexes. (biomedcentral.com)
Nearly all these proteins are subunits of known or predicted multiprotein complexes, in agreement with the balance hypothesis of evolution of gene copy number. (biomedcentral.com)
The phosphorylation of a given sugar requires four proteins, two general proteins, Enzyme I and HPr and a pair of sugar-specific proteins designated as the Enzyme II complex. (nih.gov)
Systematic mapping of multiprotein complexes formed by protein-protein interactions (PPIs) can enhance our knowledge and mechanistic basis of how proteins function in the cells. (rstudio.org)
Recent highlights include: the structure and function of long non-coding RNAs , the structure of the integrator complex , the determination of the mechanism of action of secreted Legionella effector proteins , and the structural and mechanistic analysis of the transcription/replication machines of influenza virus and lassa virus . (embl.org)
It will explore several children and adults' cancers characteristics from the structure of underlying molecular complexes, spatio-temporal genes and proteins expression patterns, all the way up to subcellular organization, tissues morphology/rheology, and ultimately drug design and screening. (nanotumor.fr)
Over the past decade, it became evident that proteins perform critical functions as components of specialized macromolecular complexes. (princeton.edu)
We find that the assembly kinetics of both proteins display complex multimodal behavior not readily quantifiable with other methods. (tamu.edu)
Geny Sylvain, Pichard Simon, Brion Alice, Renaud Jean-Baptiste, Jacquemin Sophie, Concordet Jean-Paul & Poterszman A. , décembre 2021 - « Tagging Proteins with Fluorescent Reporters Using the CRISPR/Cas9 System and Double-Stranded DNA Donors » in Multiprotein Complexes . (mnhn.fr)
Small angle neutron scattering (SANS) provides unique insight into biomacromolecular complexes by combining solvent contrast variation (H2O:D2O exchange) with either natural contrast between different classes of biomolecules (proteins, RNA/DNA, lipids/detergents) and/or by applying artificial contrast, i.e. deuteration of specific biomolecules. (lu.se)
Scaffold modules known as aminoacyl-tRNA synthetase (aaRS)-interacting multifunctional proteins (AIMPs), such as AIMP1/p43, AIMP2/p38 and AIMP3/p18, are important in driving the assembly of multi-aaRS (MARS) complexes in eukaryotes. (diamond.ac.uk)

A pan-plant protein complex map reveals deep conservation and novel assemblies. (github.io)
To capture high-quality PPIs from CF-MS co-elution profile, we have developed a well standardized and fully automated CF-MS data analysis software toolkit, referred to as MACP (Macromolecular Assemblies from the Co-elution Profile) in an open-source R package, beginning with the processing of raw co-elution data to reconstruction of high-confidence PPI networks via supervised machine-learning and underlying protein complexes using unsupervised approach. (rstudio.org)
The size, form, and distribution of these assemblies can be heterogeneous and complex, rendering their detailed characterization difficult. (tamu.edu)

We have reconstituted a number of these complexes by co-expressing multiple subunits in bacteria and studied their biochemical properties. (uni-due.de)
Identifying direct contacts between protein complex subunits from their conditional dependence in proteomics datasets. (github.io)

With the rapid progress and improvements in the fields of macromolecular crystallography, Cryo-EM, small angle scattering, electron diffraction and use of XFELs, the new LINXS theme INTEGRATIVE STRUCTURAL BIOLOGY is formed to advance cutting-edge research and to encourage new users to utilize integrative structure biology to address key scientific questions. (lu.se)
The first symposium, which is the kick-off event for the new LINXS theme, will focus on advanced, cutting-edge research in the fields of macromolecular crystallography, Cryo-EM, small angle scattering, electron diffraction, mass-spectroscopy, NMR, and use of XFELs etc. (lu.se)
In a first couple of examples, I will show how distance and shape restraints from SANS have helped to improve the uniqueness of structural models for two multi-protein-RNA complexes, in combination with NMR restraints and building blocks from crystallography [1, 2]. (lu.se)

It is one of four components of the glycine decarboxylase complex. (ucdenver.edu)

The kinetochore is a complex macromolecular machine that hierarchically assembles from a set of conserved multi-protein complexes ( Figure 1 ). (uni-due.de)
ProComp, the focus of this manuscript, represents multi-protein complexes, with an initial (but not exclusive) emphasis on protein components of complexes in mouse and human. (biomedcentral.com)

The efforts in these three research areas were accompanied by approaches to design or reprogram macromolecular complexes and new methods developed to expand the already strong expertise. (wikipedia.org)
The five research areas of CEF included: (A) Structure, mechanisms and dynamics of complexes in the membrane, (B) Composition and dynamics of macromolecular complexes in quality control and signalling, (C) Dynamics of ribonucleic acid-protein-complexes, (D) Design of macromolecular complexes, and (E) Methods for studying macromolecular complexes. (wikipedia.org)
2 Likewise, a paper in Nature Methods observed that "[m]ost cellular functions are executed by protein complexes, acting like molecular machines. (caseyluskin.com)

MBL Grenoble's researchers focus mainly on RNA biology and infection biology, in particular on the structural molecular biology of protein-RNA complexes involved in cellular gene expression and host-pathogen interactions. (embl.org)
Structural inventory of cotranslational protein folding by the eukaryotic RAC complex, Structural inventory of cotranslational protein folding by the eukaryotic RAC complex, Nat. (db-engine.de)
Comprehensive characterization of complex structural variations in cancer by directly comparing genome sequence reads. (irbbarcelona.org)

Protein complexes are distinguished from protein-protein interactions in that they are continuant entities, i.e. they endure or continue to exist through time. (biomedcentral.com)
PACSAB: Coarse-Grained Force Field for the Study of Protein-Protein Interactions and Conformational Sampling in Multiprotein Systems. (irbbarcelona.org)

Examples for important membrane complexes include the atomic structures of complex I and the ATP synthase of the mitochondrial respiratory chain and of the transporter associated with antigen processing (TAP). (wikipedia.org)
We employ high-resolution NMR studies of large chaperone-client complexes to provide an atomic resolution description of their structure and conformation and understand molecular mechanisms underlying their function. (unibas.ch)
We want to derive quantitative predictors for chaperone function, unravel atomic-level details of how clients are recognized, understand how the interplay works between chaperone and client dynamics and study how basic chaperone function is embedded into complex functional cycles. (unibas.ch)
Published macromolecular structure data files ( Atomic coordinate files , often in the PDB file format ) contain the Asymmetric Unit , which may be identical with the biological unit, or only a portion of it, or may contain multiple biological units. (proteopedia.org)

The Cluster of Excellence Frankfurt "Macromolecular Complexes" (CEF) was established in 2006 by Goethe University Frankfurt together with the Max Planck Institute of Biophysics and the Max Planck Institute for Brain Research in the context of the German Universities Excellence Initiative. (wikipedia.org)
NovaDock will then predict the three-dimensional structure of the multiprotein complex and provide energy score, cluster size, and number of ligand contacts for each model for analysis. (dnastar.com)

Logical and semantic access to related protein forms is critical for advancing bioinformatics approaches to representing, modeling, and reasoning about complex biological systems at the genomic and cellular level [ 1 ]. (biomedcentral.com)

Negative stain electron microscopy of Dam1 complex decorating microtubules. (uni-due.de)

An enzyme complex that catalyzes the transfer of GALACTOSE from UDP GALACTOSE to GLUCOSE, forming LACTOSE. (ucdenver.edu)
The enzyme complex is composed of a B subunit, ALPHA-LACTALBUMIN, which changes the substrate specificity of the A subunit, N-ACETYLLACTOSAMINE SYNTHASE, from N-ACETYLGLUCOSAMINE to glucose making lactose synthesis the preferred reaction. (ucdenver.edu)
Skeletal muscle MnSOD, mitochondrial complex II, and SIRT3 enzyme activities are decreased in maternal obesity during human pregnancy and gestational diabetes mellitus. (ouhsc.edu)
In a second example, the stoichiometry and internal topology of a highly symmetric, hetero-dodecameric aminopeptidase enzyme complex is revealed by SANS, and conclusions on the assembling process can be drawn in combination with EM data [3]. (lu.se)

Loss of Pink1 modulates synaptic mitochondrial bioenergetics in the rat striatum prior to motor symptoms: concomitant complex I respiratory defects and increased complex II-mediated respiration. (ouhsc.edu)

CryoEM structures of two spliceosomal complexes: starter and dessert at the spliceosome feast. (uchicago.edu)
The most complicated macromolecular machine in the cell may be the eukaryotic spliceosome, which removes non-coding regions (introns) from precursor messenger RNA (mRNA) in a process called splicing. (catsboard.com)

Many essential cellular functions depend on the assembly and disassembly of macromolecular complexes. (tamu.edu)

A major contribution of PRO as a protein biology community informatics resource is that it provides a formal ontological structure with foundation in Basic Formal Ontology http://www.ifomis.org/bfo/ to describe types of protein complexes and gives these types unique, permanent identifiers http://www.obofoundry.org/id-policy.shtml . (biomedcentral.com)
C) Structure of the BAM-darobactin complex. (unibas.ch)
In Situ Imaging and Structure Determination of Biomolecular Complexes Using Electron Cryo-Tomography. (uchicago.edu)
When publishing a macromolecular structure, the authors may elect to specify the biological unit. (proteopedia.org)
Modeling a protein-protein docking interaction is generally more difficult than predicting the structure of an individual protein, and because of its complex nature, accuracy can be a concern. (dnastar.com)
A docking algorithm must consider the structure of each individual protein molecule and use this information to predict the structure of the bound complex, while also considering protein flexibility, multiple point interactions between the ligand and receptor, molecular energy and biophysical interactions. (dnastar.com)

A state-of-the-art Eukaryotic Expression Facility is also available at EMBL Grenoble, which features expression of multiprotein complexes in insect and mammalian cells. (embl.org)

A TROPONIN complex subunit that binds calcium and help regulate calcium-dependent muscle contraction. (nih.gov)

PRO facilitates robust annotation of variations in composition and function contexts for protein complexes within and between species. (biomedcentral.com)
In the GO, types of protein complexes are defined in terms of constituent macromolecule classes and the function(s) that the complexes carry out. (biomedcentral.com)

Darobactin binds the lateral gate of BamA in the BAM complex by β-strand augmentation. (unibas.ch)
The Origin Recognition Complex (ORC) binds to sites in chromosomes to specify the location of origins of DNA replication. (imperial.ac.uk)

Important structures of macromolecular complexes were determined in CEF. (wikipedia.org)
The explicit representation of protein complexes in PRO--defining each member of the complex at the level of its isoform, variant, or modified form--provides the ability to represent complex biological knowledge as it is emerging in the experimental research community in structures that are both human readable and accessible to algorithmic approaches. (biomedcentral.com)
As a paper in Nature Reviews Molecular Cell Biology states, "Today biology is revealing the importance of 'molecular machines' and of other highly organized molecular structures that carry out the complex physico-chemical processes on which life is based. (caseyluskin.com)
Crystal structures of Rea1-MIDAS bound to its ribosome assembly factor ligands resembling integrin-ligand-type complexes. (db-engine.de)

A flavoprotein oxidase complex that contains iron-sulfur centers. (ouhsc.edu)

PYRUVATE DEHYDROGENASE ( LIPOAMIDE )- PHOSPHATASE catalyzes reactivation of the complex. (nih.gov)

Light sheet fluorescence microscopy for the observation of development and LILBID mass spectrometry for the analysis of membrane complexes was improved. (wikipedia.org)

This study provides a proof of concept for the application of this method on large complexes. (cnrs.fr)

Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. (uchicago.edu)

Multiprotein complexes that carry out PROTEIN TRANSPORT across CELL MEMBRANES. (bvsalud.org)
Multiprotein complexes that carry out protein SECRETION PROCESSES. (bvsalud.org)

Here, we discuss a recent study by Wan and colleagues, which highlights the significance of protein complexes by studying their conservation in organisms separated by up to a billion years of evolution. (princeton.edu)

Many of the cell's macromolecular machines appear gratuitously complex, comprising more components than their basic functions seem to demand. (catsboard.com)

We describe here how the PRO Consortium is meeting the challenge of representing species-specific protein complexes, how protein complex representation in PRO supports annotation of protein complexes and comparative biology, and how PRO is being integrated into existing community bioinformatics resources. (biomedcentral.com)

100th Anniversary of Macromolecular Science Viewpoint: Data-Driven Protein Design. (uchicago.edu)

This analysis has already yielded some important insights: For example, the Dam1 complex, a specialized microtubule-binding component of the budding yeast kinetochore oligomerizes to form a ring around microtubules in vitro ( Figure 2 ). (uni-due.de)

Specifically, we exploit the highest signal-to-noise light bursts from single fluorescent particles transiting a confocal excitation spot to recursively determine the brightness and size distribution of complex mixtures of fluorescent objects. (tamu.edu)

Open questions remain: how exactly does the transition from chaperone to BAM complex occur? (unibas.ch)

Our investigations into kinetochore assembly have led to the identification of a conserved receptor molecule for the microtubule-binding Ndc80 complex. (uni-due.de)

Anatomy28

Organisms12

Chemicals and Drugs117

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes65

Disciplines and Occupations3

Information Science9

Substances4

Proteins15

Assemblies3

Subunits2

Crystallography3

Glycine Decarbo1

Multi-protein complexes2

Methods3

Structural3

Protein-protein inte2

Atomic4

Cluster2

Biological systems1

Electron microscopy1

Enzyme4

Respiratory1

Spliceosome2

Cellular functions1

Structure6

Eukaryotic1

Subunit1

Function2

Binds2

Structures4

Oxidase1

Catalyzes1

Membrane1

Concept1

Large1

Carry2

Conservation1

Components1

Describe1

Data1

Insights1

Size1

Chaperone1

Assembly1