Lysozyme. Medical search. Frequent questions

In humans, the C-type lysozyme enzyme is encoded by the LYZ gene. (wikipedia.org)
They coupled the hydrolytic enzyme lysozyme with SOD-active Cu(II) complex to form the hybrid lysozyme CuST@lysozyme, which showed promising SOD activity but low biotoxicity. (tus.ac.jp)
A 1-4-Beta-N-Acetylmuramidase comprising 129 amino acids, LYSOZYME is a small enzyme that potently cuts Gram-positive bacteria's exposed outer protective peptidoglycan coating. (bioseutica.com)
Lysozyme is a catalytic enzyme that digests bacterial cell wall and is found in significant amounts in egg whites. (ukessays.com)
Lysozyme was the first enzyme to have its structure solved, back in 1965. (srcf.net)
Lysozyme is a 14.4 kDa enzyme, commonly referred to as the "body's own antibiotic" since it kills bacteria. (diagnostictechnology.com.au)
Lysozyme is an enzyme that destroys bacterial cell walls by hydrolyzing the polysaccharide component of the cell wall. (diagnostictechnology.com.au)
Lysozyme is a relatively small enzyme consisting of a 129 amino acid polypeptide chain with a molecular weight of 14.6 kDa. (ilexlife.com)
Johan Brouwer, Trudi van Leeuwen-Herberts und Marjo Otting-van de Ruit: Determination of lysozyme in serum, urine, cerebrospinal fluid and feces by enzyme immunoassay. (ilexlife.com)
Lysozyme (LYSO) is an enzyme that is upregulated in many organisms during the innate immune response against infection by bacterial pathogens. (oncotarget.com)
In recent years, several oconjugation protocols have been developed to improve the functional properties of the enzyme lysozyme. (ac.ir)
Initially, inulin (MW about 25 kDa) was oxidized by periodate to provide the reactive carbonyl groups to attach to amino groups of lysozyme for Maillard reaction and was then conjugated to the enzyme at a molar ratio of oxidized inulin to enzyme of 5:1. (ac.ir)
The results showed that under these conditions, the inulin-lysozyme conjugate had 58% of the lytic activity of the native enzyme and had better emulsifying properties and heat stability than native lysozyme. (ac.ir)
For example, the mucous membranes of the eyes are bathed in tears, which contain an enzyme called lysozyme that attacks bacteria and helps protect the eyes from infection. (msdmanuals.com)

Our analysis indicates that the Ag nanoparticles most likely interact with Trp-123 which is in close proximity to Phe-34 of the lysozyme molecule. (arxiv.org)
It is hypothesized that since lysozyme is a small molecule in comparison to the fractionation range of the Sehadex G-50 coloumn, a significant amount of lysozyme is likely to be trapped or retarded by the resin beads resulting in a low yield. (ukessays.com)
One of the world's smallest transistors, constructed from a single carbon nanotube, was attached to a molecule of lysozyme, a bacterium-fighting protein. (srcf.net)

Sorption calorimetry experiments demonstrate that the hydration behavior of this formulation is similar to that of the pure amorphous sucrose, while the presence of lysozyme only shifts the sucrose transitions. (lu.se)
Substrate binding is used as a powerful tool in this experiment where the enzymatic reactions are used for detecting the presence of lysozyme. (ukessays.com)
1999). However, gram negative bacteria are less vulnerable to the presence of lysozyme due to their thin layer of peptidoglycan shielded by the outer membrane of lipopolysaccharide (Lee-Huang et al. (ukessays.com)

This behaviour is shown to be consistent with the alcohol-dependent enthalpy of denaturation of lysozyme found by Velicelebi and Sturtevant [Velicelebi and Sturtevant, Biochem. (ubc.ca)
In this contribution we will present an investigation of the effect of the hydration level on the denaturation of lysozyme induced by heat treatment using DSC. (lu.se)

This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). (sigmaaldrich.com)
RePub, Erasmus University Repository: Tissue specific and position independent expression of the complete gene domain for chicken lysozyme in transgenic mice. (eur.nl)
A 21.5 kb DNA fragment carrying the entire chicken lysozyme gene locus was introduced into the germ line of mice. (eur.nl)

LYSOZYME is positively charged (cationic), so it is attracted to the negatively charged lipopolysaccharides (LPSs) of the outer membrane of Gram-negative bacteria 1 . (bioseutica.com)
Moreover, when lysozyme is injected into zein-film in combination with EDTA, it enhances the antimicrobial effectiveness against the Gram-negative bacteria Escherichia coli , resulting in an extended duration of food shelf life 2 . (bioseutica.com)
2002). Gram positive bacteria are more susceptible to the effects lysozyme due to their peptidoglycan cell wall being exposed to the extracellular environment (Lee-Huang et al. (ukessays.com)
Lysozyme is a glycosidase that, as a component of the innate immune system, is primarily directed against the murein-containing cell wall of gram-positive bacteria. (ilexlife.com)
It results from the conducted examinations concerning chicken breast muscles that lysozyme exhibited inhibitory action towards aerobic and indicator bacteria. (olsztyn.pl)
The application of a lysozyme solution with the activity of 12 000 U/ml proved especially effective, as it reduced the number of aerobic bacteria twenty times. (olsztyn.pl)

Like other Alexa Fluor dyes, the Anti-Lysozyme exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. (ningbobio.com)

The conjugate families were more hydrophobic than native lysozyme. (kent.ac.uk)

Using differential scanning calorimetry, thermogravimetric analysis, sorption calorimetry, and synchrotron small-angle X-ray scattering (SAXS), we have characterized the properties at low (re)hydration levels of the protein lysozyme, which was freeze-dried together with the excipient sucrose. (lu.se)

This project will use genomics-derived technology to economically produce lysozyme formulations that will be used to improve gut health and productivity in food animals. (genomecanada.ca)
Within 24 h of infection, cells found in the marginal zone of the spleen begin to produce lysozyme mRNA. (ox.ac.uk)

SAXS analysis of amorphous lysozyme-sucrose mixtures and unfolding of lysozyme in this environment show that prior to unfolding, the size and shape of lysozyme in a solid sucrose matrix are consistent with its native state in an aqueous solution. (lu.se)
To help understand how sugar interactions with proteins stabilise biomolecular structures, we compare the three main hypotheses for the phenomenon with the results of long molecular dynamics simulations on lysozyme in aqueous trehalose solution (0.75 M). We show that the water replacement and water entrapment hypotheses need not be mutually exclusive, because the trehalose molecules assemble in distinctive clusters on the surface of the protein. (aston.ac.uk)
The oligomerization of lysozyme in aqueous solution was investigated by Monte Carlo simulations as a function of protein concentration, pH, and electrolyte screening. (diva-portal.org)
Excess partial molar enthalpies were measured in ternary aqueous solutions, tert-butyl alcohol (TBA)DMSO-H2O, and lysozyme (L)-alcohols (A)-H2O. (ubc.ca)

The restrictive nature of Lysozyme antibody staining suggests that Lysozyme may be synthesized predominantly in reactive histiocytes rather than in resting, unstimulated phagocytes. (diagnostictechnology.com.au)
This is a highly specific antibody against Lysozyme. (ningbobio.com)

as a result, the observed increase in the T g of the mixtures is the consequence of the confinement of the amorphous sucrose domains in the interstitial space between the lysozyme molecules. (lu.se)

The value of the measurement of cerebrospinal fluid levels of lysozyme in the diagnosis of neurological disease. (bmj.com)
A turbidimetric technique has been adapted to yield maximum sensitivity for the measurement of lysozyme in cerebrospinal fluid. (bmj.com)
Lysozyme is found in many secretions of the human body, including tear fluid, saliva, blood serum and cerebrospinal fluid. (ilexlife.com)

However, fecal lysozyme may occur through intestinal granulocytes. (ilexlife.com)

We showed earlier that the mutation of Leu99 to alanine in bacteriophage T4 lysozyme creates an internal cavity of volume approximately 150 A(3) that binds benzene and a variety of other ligands. (nih.gov)
They report that the His15 imidazole group of the lysozyme binds to the Cu(II) center of CuST in the equatorial position while the CuST unit is fixed axially by several weak coordination and hydrogen bonds. (tus.ac.jp)
Lysozyme in sample, standard and controls binds to antibodies, which are coated to the microtiter plate. (ilexlife.com)

The mitogenic potential of the reactive material decreased after lysozyme digestion, indicating that part of the material was peptidoglycan. (cdc.gov)

1999). Lysozyme comprises about 3.5 % of the total protein weight in albumen (Lee-Huang et al. (ukessays.com)
1999) and thus a solution of egg white was used as the source of lysozyme in this lab. (ukessays.com)

In this article, we discuss metal-protein interactions in the Ag-lysozyme complex by spectroscopic measurements. (arxiv.org)

This results from an increase in equilibrium water content with lysozyme concentration at constant water activity. (lu.se)
Furthermore, we also observed an increase in the glass transition temperature (T g ) of the mixtures with increasing lysozyme concentration. (lu.se)
of the mixtures with increasing lysozyme concentration. (lu.se)
The highest lysozyme activity was observed at a substrate concentration of 0.4 mg/mL and at an optimum phosphate buffer pH of 7. (ukessays.com)
The protein assay indicated a significant concentration of protein in the neighbouring fractions of purified lysozyme, however the exact concentration of lysozyme in albumen remain inconclusive. (ukessays.com)
The main objective of this lab is to purify lysozyme using size exclusion chromatography for examining its structural properties and concentration in albumen. (ukessays.com)

In this study, the optimum conditions of lysozyme - oxidized inulin conjugation and functional properties of the modified lysozyme were investigated. (ac.ir)
A number of studies were conducted to investigate the optimization of lysozyme - Inulin conjugation consisting of different pH levels (3, 7, and 9), different temperatures (40, 60, and 80°C), and different reaction times. (ac.ir)

Perhaps best known for its presence in saliva and tears, LYSOZYME holds a critical front-line defense role for plants and animals. (bioseutica.com)

All of these expressed chicken lysozyme RNA at high levels specifically in macrophages, as is the case in the donor species. (eur.nl)
Furthermore, lysozyme can be actively secreted into the intestinal lumen by monocytes and macrophages. (ilexlife.com)
This study demonstrates the induction of lysozyme mRNA expression in situ in tissue macrophages (M phi) of mice following in vivo stimulation. (ox.ac.uk)

The structural composition of lysozyme consists of 129 amino acid residues folded into a compact globular structure with a cleft for substrate binding (Berg et al. (ukessays.com)

This lab investigates the purification and assaying of lysozyme using size exclusion chromatography and Bradford protein assay. (ukessays.com)
Size exclusion chromatography provides purification of lysozyme, however about 64% of its initial yield is lost in the process of purification through the beads. (ukessays.com)
2000). The different proteins present in albumen should elute at different fractions due to their varying molecular sizes leading to the purification of lysozyme which has a literature structural mass of 14,400da. (ukessays.com)

Since its discovery by Alexander Fleming in 1921, LYSOZYME remains one of the most potent natural antimicrobial compounds identified to date. (bioseutica.com)
The food industry has taken advantage of the natural antimicrobial properties of LYSOZYME, incorporating it into processing methods and using it as a preservative. (bioseutica.com)

Conclusion: The quantification of lactoferrin and lysozyme enabled an assessment of possible associations with caries status, thus improving the understanding of the biological and etiological aspects of caries. (pucrs.br)
Quantification of fecal lysozyme may be useful in researching inflammatory processes in the intestine and for the detection of a disturbed immunological barrier at the intestinal mucosa. (ilexlife.com)

The results show minimal impact of polysorbate 20 on lysozyme structural changes during freeze and thaw using practically relevant concentrations. (nist.gov)
There was a slight association between lysozyme concentrations and DMFT. (pucrs.br)
Micrococcus Lysodeikticus is employed in this lab as the substrate for lysozyme and its enzymatic activities are observed under various pH and substrate concentrations for determining the optimal conditions for lysozyme activity. (ukessays.com)

The association between caries experience/activity and the electrophoretic profiles of salivary proteins lactoferrin and lysozyme was evaluated. (pucrs.br)
[ 2 ] Research has shown that almost all patients with familial renal amyloidoses (FRA) are heterozygous for mutations in the genes for lysozyme, apolipoprotein AI, apolipoprotein AII, or fibrinogen A alpha-chain and that the amyloid fibrils in this condition are derived from the respective variant proteins. (medscape.com)

Now, researchers from Japan have designed a novel lysozyme coupled Cu(II) hybrid protein complex that demonstrates enhanced SOD activity and excellent stability. (tus.ac.jp)
We chose lysozyme owing to its stability and crystallinity. (tus.ac.jp)
Through assays, the researchers established high SOD activity and stability of the biocompatible CuST@lysozyme hybrid protein complex. (tus.ac.jp)
They further suggest that the stability of the complex can be improved by suppressing ligand dissociation by using late-transition-metal complexes for binding lysozyme, increasing interaction between the complexes and lysozyme by using ligands with hydrogen-bonding moieties, and introducing acidic functional groups to counter the basic side chains of lysozyme. (tus.ac.jp)
We have strategically improved the stability of the metal-lysozyme composites, specifically in biological fluids such as plasma and cytosol. (tus.ac.jp)
The aim of the study was to assess the effect of lysozyme with varying activity on the microbiological stability and sensory attributes of chicken breast muscles. (olsztyn.pl)

Through detailed crystallographic and spectroscopic analysis, the research team, which also comprised Assistant Professor Kenichi Kitanishi from TUS, Dr. Arshak Tsaturyan from Southern Federal University, and Professor Masaki Unno from Ibaraki University, among others, confirmed the formation of the hybrid protein CuST@lysozyme, and elucidated its structure. (tus.ac.jp)

Its potent bacteriolytic, bacteriostatic, and bactericidal action has made LYSOZYME highly valued across broad industries, applied in products ranging from cheese, beer, and wine to toothpaste and eyedrops. (bioseutica.com)

Chitin has also been shown to be a viable lysozyme substrate. (wikipedia.org)

Bioseutica ® has developed LYSOLAC ® , LYSOVIN ™ , and LYSOBIER ® , formulations of LYSOZYME tailored to cheesemaking , winemaking , and beer brewing . (bioseutica.com)

Lysozyme from chicken egg white has been used for the extraction of genomic DNA from bacterial cells. (seraglob.com)

Hydration of lysozyme studied by Raman spectroscopy. (lu.se)

Full-matrix least-squares refinement of lysozymes and analysis of anisotropic thermal motion. (expasy.org)

Lytic effect of trypsin, lysozyme, and complement on Treponema pallidum. (bmj.com)

However, lysozyme in human milk loses activity very quickly at that temperature. (wikipedia.org)
The isoelectric point of human milk lysozyme is 10.5-11. (wikipedia.org)

In this work, we studied the influence of non-ionic surfactants (polysorbate 20 and poloxamer 188) on protein structural changes during freeze and thaw using lysozyme as a model protein. (nist.gov)
The highest lysozyme activity was observed for fraction # 12 indicating a structural mass range of 15,000da-13,500da for lysozyme. (ukessays.com)

Hen egg white lysozyme maintains its activity in a large range of pH (6-9). (wikipedia.org)
Lysozyme, when integrated into PVOH (polyvinyl alcohol) films, extends the release of antimicrobials, effectively inhibiting microbial activity. (bioseutica.com)
These optimal conditions were set as standard conditions for assaying the purified fractions for lysozyme activity and for the protein assay. (ukessays.com)

Lysozyme is an inducible marker of macrophage activation in murine tissues as demonstrated by in situ hybridization. (ox.ac.uk)
The resting resident tissue M phi of most tissues do not contain enough lysozyme mRNA to be detected by in situ hybridization using 35S-labeled RNA probes. (ox.ac.uk)

Following Bacille Calmette Guerin or Plasmodium yoelli infection, however, M phi recruited to liver and spleen hybridize strongly to the lysozyme probe. (ox.ac.uk)

Large amounts of lysozyme can be found in egg white. (wikipedia.org)
Hen egg white lysozyme is thermally stable, with a melting point reaching up to 72 °C at pH 5.0. (wikipedia.org)
This abundance in hen's egg-white was first identified in 1922, and it remains one of the most LYSOZYME-rich sources known to man. (bioseutica.com)
A solution of egg white is used as the source for lysozyme and it is purified using size exclusion chromatography with column Sephadex G-50 that has a fractionation range from 1,500 to 30,000 daltons. (ukessays.com)
Later in the infection, other cells in the red and white pulp of the spleen, and cells in granulomas in the liver, become lysozyme-positive. (ox.ac.uk)
Estrone glucuronide conjugates of hen egg white lysozyme were prepared by both the mixed-anhydride and active-ester coupling procedures. (kent.ac.uk)

C-type lysozymes are closely related to α-lactalbumin in sequence and structure, making them part of the same glycoside hydrolase family 22. (wikipedia.org)

In this experiment lysozyme is extracted and purified from albumen using size exclusion chromatography. (ukessays.com)
Lysozyme solutions with the activities of 2 400, 6 000 and 12 000 U/ml, applied in the experiment, were sprayed onto the surface of the muscles. (olsztyn.pl)

However, knowing the structure alone is not enough to explain how lysozyme works - the way it moves is important too. (srcf.net)
Methionine and alanine substitutions show that the formation of wild-type-like structure in the carboxy-terminal domain of T4 lysozyme is a rate-limiting step in folding. (expasy.org)

Lysozyme is normally undetectable or detectable in small amounts in the intestinal contents. (ilexlife.com)

At cryogenic temperatures encapsulated lysozyme did not show cold denaturation, instead became more structured. (asme.org)

The production of lysozyme, previously considered a constitutive function of M phi, is therefore an indicator of M phi activation in vivo, where immunologically specific and nonspecific stimuli both stimulate lysozyme production at high levels in subpopulations of cells occupying discrete anatomical locations. (ox.ac.uk)

Taken together, the results of this study indicated that lysozyme modification by oxidized inulin results in a new product with improved functional properties which may be employed for different industrial purposes. (ac.ir)
The obtained results indicated that lysozyme may be an effective agent prolonging the wholesomeness of portioned poultry meat. (olsztyn.pl)

We investigated the formation of a hybrid protein composed of lysozyme and a functional SOD-mimetic Cu(II) complex. (tus.ac.jp)

Lysozyme folds to form two 'jaws', which allow it to hold sugar chains in place in order to cut them apart by breaking a chemical bond. (srcf.net)

Anatomy18

Organisms36

Diseases2

Chemicals and Drugs65

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes43

Disciplines and Occupations3

Technology, Industry, Agriculture5

Information Science3

Health Care2

Enzyme14

Molecule3

Presence of lysozyme3

Denaturation of lysozyme2

Gene3

Bacteria6

Anti-Lysozyme1

Native lysozyme1

Protein lysozyme1

Produce lysozyme2

Aqueous4

Antibody2

Molecules1

Cerebrospinal fluid3

Granulocytes1

Binds3

Reactive1

19992

Interactions1

Concentration6

Conjugation2

Saliva1

Macrophages3

Amino1

Purification3

Antimicrobial2

Quantification2

Concentrations3

Proteins2

Stability6

Spectroscopic1

Bacteriostatic1

Substrate1

Formulations1

Chicken1

Hydration1

Refinement1

Lytic1

Human milk2

Structural2

Activity3

Tissues2

Strongly1

White6

Sequence1

Experiment2

Structure2

Amounts1

Temperatures1

High1

Results2

Complex1

Form1