AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
Interleukin-1 Receptor Accessory ProteinViral Regulatory and Accessory ProteinsAccessory NerveReceptor, Melanocortin, Type 2Ureasevpr Gene Products, Human Immunodeficiency VirusHuman Immunodeficiency Virus ProteinsGene Products, vprMolecular Sequence DataWolff-Parkinson-White SyndromeDNA-Directed DNA PolymeraseAccessory Atrioventricular BundleAmino Acid SequenceProtein BindingNickelReceptors, Interleukin-1 Type IReceptors, Interleukin-1ClathrinReceptors, Interleukin-1 Type IIViral ProteinsT-PhagesHydrogenaseBacterial ProteinsAccessory Nerve InjuriesEnterobacter aerogenesCell Linevif Gene Products, Human Immunodeficiency VirusBase SequenceCarrier ProteinsHIV-1Adaptor Protein Complex gamma SubunitsGenitalia, MaleProtein Structure, Tertiarynef Gene Products, Human Immunodeficiency VirusMutationMembrane ProteinsBinding SitesDNA ReplicationBacteriophage T4Clathrin-Coated VesiclesSARS VirusReceptor Activity-Modifying ProteinsGene Products, vifAdaptor Protein Complex delta SubunitsAdaptor Proteins, Vesicular TransportDNA-Binding ProteinsEscherichia coliModels, MolecularTemplates, GeneticPre-Excitation SyndromesAdaptor Protein Complex 2DNA Polymerase IIIHeLa CellsMonomeric Clathrin Assembly ProteinsVirus ReleaseVirus ReplicationAdaptor Protein Complex beta SubunitsThiocapsaRecombinant Fusion ProteinsCercopithecus aethiopsReplication Protein CPlasmidsSequence Homology, Amino AcidProtein TransportEndocytosisTwo-Hybrid System TechniquesAtrioventricular NodeSignal TransductionCloning, MolecularProteinsCell MembraneVomeronasal OrganGPI-Linked ProteinsOpen Reading FramesInterleukin-1Bacteriophage phi X 174Accessory Nerve DiseasesMonomeric GTP-Binding ProteinsRecombinant ProteinsRetroviridae ProteinsAdaptor Protein Complex alpha SubunitsGenetic Complementation TestAmino Acid MotifsApoenzymesVirionTachycardia, SupraventricularCoated Pits, Cell-MembraneKineticsDNA, ViralProtein ConformationTransfectionHeart Conduction SystemHEK293 CellsModels, BiologicalDimerizationProliferating Cell Nuclear AntigenHIV-2Vesicular Transport ProteinsCatheter AblationT-Lymphocytes
KinaseEndoplasmic reticulumDegradationMembranesELISA KitViral ProteinsRegulatesReceptorMembrane proteinsEnzymeTargetsCompartmentCalciumRegulateAmino acidsGenesSecretory proteinTransmembrane proteinCharacterizationPathwaysInteractsIntracellularLocalizationGolgiMutationsPlasmaGeneLysosomeGenomeDanonGTPaseCellularActinRegulationApoptosisCellsFoundDeficiencyInteractionCompositionHumanDiseasesDomainTransportDisease
Kinase6
  • Calcium stimuliert die Calmodulin dependant Protein Kinase wodurch für die Aldosteron Synthese wichtige Transkriptionsfaktoren phosphoryliert werden wie zum Beispiel Hydroxylase cytochrome P450 (CYP11B2) 4 . (antikoerper-online.de)
  • Since ER stress can cause differential modulation of the unfolded protein response (UPR), which includes the inositol-requiring enzyme 1 (IRE-1), activating transcription factor 6 (ATF6) and PKR-like ER kinase (PERK) pathways, these were individually tested in 3a-expressing cells. (nih.gov)
  • These diseases are a result of storage in cellular vacuoles and include Danon disease, Pompe disease, Fabry disease , and a form of HCM related to a mutation in the adenosine monophosphate (AMP)-activated, gamma-2 noncatalytic subunit of protein kinase ( PRKG2 ). (medscape.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Death Associated Protein Kinase 1 (DAPK1) in Tissue homogenates and other biological fluids. (tuberculosisdrugresearch.org)
  • Description: A sandwich ELISA kit for detection of Death Associated Protein Kinase 1 from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (tuberculosisdrugresearch.org)
  • Description: A sandwich quantitative ELISA assay kit for detection of Human Death Associated Protein Kinase 1 (DAPK1) in samples from tissue homogenates, cell lysates or other biological fluids. (tuberculosisdrugresearch.org)
Endoplasmic reticulum3
  • Since the 3a protein localizes to the endoplasmic reticulum (ER)-Golgi compartment, its role in causing ER stress was investigated in transiently transfected cells. (nih.gov)
  • One-third of the mammalian proteome is comprised of transmembrane and secretory proteins that are synthesized on endoplasmic reticulum (ER). (janelia.org)
  • We focused on Orf6, which was most cytotoxic and localized to the endoplasmic reticulum, autophagosome and lysosomal membranes. (biomedcentral.com)
Degradation3
  • The 3a protein was found to induce serine phosphorylation within the IFN alpha-receptor subunit 1 (IFNAR1) degradation motif and to increase IFNAR1 ubiquitination. (nih.gov)
  • Cav-2beta isoform yielded by alternative translation initiation desensitizes insulin receptor (IR) via dephosphorylation by PTP1B, and subsequent endocytosis and lysosomal degradation of IR, causing insulin resistance. (cusabio.com)
  • Mechanistically, we found that Rab40c binds the protein phosphatase 6 (PP6) complex and ubiquitylates one of its subunits, ankyrin repeat domain 28 (ANKRD28), thus leading to its lysosomal degradation. (life-science-alliance.org)
Membranes2
  • Topics such as lysosomal dynamics, the biophysical challenges of bending membranes, and the evolution of endocytic systems are also covered. (cshlpress.com)
  • May act as a scaffolding protein within caveolar membranes. (cusabio.com)
ELISA Kit1
  • Description: A sandwich ELISA kit for detection of Protein Tyrosine Phosphatase, Non Receptor Type 11 from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (worldcarecouncil.org)
Viral Proteins1
Regulates4
  • The GTPase Rab11 regulates endosomal protein trafficking and previous work has shown that loss of Rab11 caused intestinal inflammation and hyperplasia in mice and flies. (sdbonline.org)
  • Since adenosine A 2A receptors (A 2A Rs) control lysosome trafficking and pH, which closely regulates lysosomal calcium, we hypothesized a role for these receptors in NPC1. (jneurosci.org)
  • Lysosomal release of amino acids at ER three-way junctions regulates transmembrane and secretory protein mRNA translation. (janelia.org)
  • Altogether, these findings uncover a novel coordination between ER and lysosomes, in which local release of amino acids and other factors from ER-associated lysosomes patterns and regulates translation of mRNAs encoding secretory and membrane proteins. (janelia.org)
Receptor4
  • V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. (wikipedia.org)
  • G protein-coupled receptor 85 [Sour. (gsea-msigdb.org)
  • Description: A sandwich quantitative ELISA assay kit for detection of Human Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11) in samples from tissue homogenates or other biological fluids. (worldcarecouncil.org)
  • The sentence should read: Recently, it was shown that GGAs and the related clathrin adaptor AP-1 complex have functions not only in TGN-endosomal/lysosomal membrane trafficking, but also for the surface expression of a series of integral membrane proteins, including Notch and the epidermal growth factor receptor [13,21, Uemura et al. (googleapis.com)
Membrane proteins1
Enzyme6
  • Renin ( etymology and pronunciation ), also known as an angiotensinogenase , is an aspartic protease protein and enzyme secreted by the kidneys that participates in the body's renin-angiotensin-aldosterone system (RAAS)-also known as the renin-angiotensin-aldosterone axis-that increases the volume of extracellular fluid ( blood plasma , lymph and interstitial fluid ) and causes arterial vasoconstriction . (wikipedia.org)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Dynein Heavy Chain Domain Containing Protein 1 (DNHD1) in samples from Tissue homogenates and other biological fluids. (youngresearch.eu)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Surfactant Associated Protein D (SPD) in serum, plasma, lung lavage fluid and other biological fluids. (2sars.com)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Surfactant Associated Protein D (SPD) in samples from serum, plasma, lung lavage fluid and other biological fluids with no significant corss-reactivity with analogues from other species. (2sars.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Patatin Like Phospholipase Domain Containing Protein 2 (PNPLA2) in tissue homogenates, cell lysates and other biological fluids. (resourcerecovery.be)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Patatin Like Phospholipase Domain Containing Protein 2 (PNPLA2) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (resourcerecovery.be)
Targets3
  • In addition to the lysosomal compartment, mitochondria have been described as relevant targets of the NPC1 pathogenesis. (jneurosci.org)
  • However, the exact functions of Rab40c remain to be determined, and what proteins are the targets of Rab40c-Cullin5-mediated ubiquitylation in mammalian cells are unknown. (life-science-alliance.org)
  • Understanding how SARS-CoV-2 hijacks host machinery and which of its proteins are key for its interaction will be crucial in identifying effective targets for COVID-19 therapeutic intervention. (biomedcentral.com)
Compartment2
  • Niemann-Pick type C1 (NPC1) disease is a rare neurovisceral disorder characterized by intracellular accumulation of unesterified cholesterol, sphingolipids, and other lipids in the lysosomal compartment. (jneurosci.org)
  • Confocal microscopic analysis showed increased translocation of IFNAR1 into the lysosomal compartment and flow cytometry showed reduced levels of IFNAR1 in 3a-expressing cells. (nih.gov)
Calcium3
  • A deregulation of lysosomal calcium has been identified as one of the earliest steps of the degenerative process. (jneurosci.org)
  • We show that CGS21680 raises lysosomal calcium levels and rescues mitochondrial functionality (mitochondrial inner membrane potential and expression of the complex IV of the mitochondrial respiratory chain), which is compromised in NPC1 cells. (jneurosci.org)
  • The effects of A 2A R activation on lysosomal calcium are not mediated by the cAMP/PKA pathway but they appear to involve the phosphorylation of ERK1/2. (jneurosci.org)
Regulate3
  • This 37 kD protein can regulate cell signaling as an extracellular molecule and probably also at intracellular sites. (bvsalud.org)
  • Interacts directly with G-protein alpha subunits and can functionally regulate their activity. (cusabio.com)
  • Rab40c is a SOCS box-containing protein which binds Cullin5 to form a ubiquitin E3 ligase complex (Rab40c/CRL5) to regulate protein ubiquitylation. (life-science-alliance.org)
Amino acids1
  • This mutation shortens the translated protein from 574 to 286 amino acids, removing the helix-loop-helix DNA-binding domain that is highly conserved among vertebrates. (unige.ch)
Genes2
  • Cells expressing the 3a proteins showed ER stress based on activation of genes for the ER chaperones GRP78 and GRP94. (nih.gov)
  • The most common cause of HCM is now known to be one of over 200 possible mutations in at least 10 genes that involve sarcomeric proteins. (medscape.com)
Secretory protein2
  • Plasma levels of renalase, an anti-inflammatory secretory protein, dramatically decrease in a murine acute pancreatitis model. (bvsalud.org)
  • The survival factor renalase (RNLS) is a recently discovered secretory protein with potent prosurvival and anti-inflammatory effects. (bvsalud.org)
Transmembrane protein1
  • The most common form of albinism is caused by mutations in oculocutaneous albinism II (OCA2), a melanosome-specific transmembrane protein with unknown function. (elifesciences.org)
Characterization1
  • Results present initial characterization of key proteins Cav2 and CFL1 as cellular factors that colocalize with M in viral inclusions and filaments and ZNF502 protein which appears to interact with RSV M in the nucleus. (cusabio.com)
Pathways1
  • Disordered lysosomal/autophagy pathways initiate and drive pancreatitis, but the underlying mechanisms and links to disease pathology are poorly understood. (bvsalud.org)
Interacts2
  • Proteomics revealed Orf6 interacts with nucleopore proteins (RAE1, XPO1, RANBP2 and nucleoporins). (biomedcentral.com)
  • For example, HIV-1 Vpr protein interacts with host proteins to modify host cell energy metabolism, oxidative status and proteasome function. (biomedcentral.com)
Intracellular1
  • The correct reference is: Uemura T, Sawada N, Sakaba T, Kametaka S, Yamamoto M, Waguri S. Intracellular localization of GGA accessory protein p56 in cell lines and central nervous system neurons. (googleapis.com)
Localization3
  • Loss of Pcs impairs Rab11 localization on the trans-side of Golgi units and induces the cytoplasmic accumulation of post-Golgi vesicles bearing rhabdomere proteins, as observed in Rab11-deficiency. (sdbonline.org)
  • We examined each of the individual SARS-CoV-2 proteins for their cytotoxicity in HEK 293 T cells and their subcellular localization in COS-7 cells. (biomedcentral.com)
  • All three proteins showed membrane localization in COS-7 cells. (biomedcentral.com)
Golgi2
  • The Rab34/RILP association, which presumably is taking place at the Golgi appears to control the distant lysosomal distribution. (ximbio.com)
  • Plasma membrane and lysosomal proteins also travel from the rough ER to the Golgi and then to their final destinations. (urva-habitat.fr)
Mutations1
  • Forms of HCM without evidence of mutations in sarcomeric proteins have also been identified. (medscape.com)
Plasma1
  • Description: A sandwich quantitative ELISA assay kit for detection of Human Surfactant Associated Protein D (SPD) in samples from serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates or other biological fluids. (2sars.com)
Gene2
  • The encoded protein of this gene is approximately 45 kD and may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. (wikipedia.org)
  • Only the PERK pathway was found to be activated in 3a-expressing cells based on (1) increased phosphorylation of eukaryotic initiation factor 2 alpha (eIF2alpha) and inhibitory effects of a dominant-negative form of eIF2alpha on GRP78 promoter activity, (2) increased translation of activating transcription factor 4 (ATF4) mRNA, and (3) ATF4-dependent activation of the C/EBP homologous protein (CHOP) gene promoter. (nih.gov)
Lysosome1
  • Less secretome mRNA translation also occurred when lysosome function was perturbed by raising lysosomal pH or inhibiting lysosomal proteases. (janelia.org)
Genome2
  • Our study revealed Orf6 as a highly pathogenic protein from the SARS-CoV-2 genome, identified its key host interacting proteins, and Selinexor as a drug candidate for directly targeting Orf6 host protein interaction that leads to cytotoxicity. (biomedcentral.com)
  • The SARS-CoV-2 viral genome encodes 28 confirmed proteins. (biomedcentral.com)
Danon4
  • In 1981, Danon described a multisystemic, lysosomal, glycogen-storage disease different from the previously described Pompe disease. (medscape.com)
  • [ 2 ] Danon disease is also known as lysosomal glycogen-storage disease with normal acid maltase. (medscape.com)
  • A study by Hashem et al using induced pluripotent stem cell-derived cardiomyocytes indicated that LAMP2 protein deficiency in Danon disease results in autophagic flux impairment, which in turn causes excessive oxidative stress and, subsequently, cardiomyocyte apoptosis. (medscape.com)
  • In a study that included two girls, aged 10 and 13 years, Hedberg Oldfors et al found evidence that early onset HCM in Danon disease, in contrast to the late-onset cases that can occur, may result from an uneven distribution of LAMP2 protein in cardiac muscle, with the protein lacking in some large portions of the muscle, while its expression is preserved in other large myocardial areas. (medscape.com)
GTPase1
Cellular2
  • Enhanced mTORC1 signaling and protein synthesis in pathologic α-synuclein cellular and animal models of Parkinson's disease. (neurotree.org)
  • We set out to identify the SARS-CoV-2 pathogenic proteins that through host interactions cause the cellular damages underlying COVID-19 symptomatology. (biomedcentral.com)
Actin2
Regulation1
  • Here, we investigate the spatial distribution and regulation of mRNAs encoding these membrane and secretory proteins (termed "secretome" mRNAs) through live cell, single molecule tracking to directly monitor the position and translation states of secretome mRNAs on ER and their relationship to other organelles. (janelia.org)
Apoptosis1
Cells1
  • Many culturing strategies are based on the addition of feeder or accessory cells, which need to be removed prior to the clinical application of the final NK cell product. (frontiersin.org)
Found3
  • Results verified the presence of transcript III of cav-2 for the first time, but no protein associated was found. (cusabio.com)
  • Lysosomal-associated membrane protein-2 (LAMP2) is a heavily glycosylated protein found inside the lysosomal membrane. (medscape.com)
  • The most common type of albinism is caused by defects in a protein called OCA2, which is found in the membrane that surrounds melanosomes. (elifesciences.org)
Deficiency1
  • Studies of the human immune deficiency virus (HIV-1) over the past 30 years have demonstrated the presence of prime pathogenic proteins that contribute to virulence and host disease progression, and the potential of targeting these proteins for effective therapeutic intervention [ 9 ]. (biomedcentral.com)
Interaction1
  • We also used Mass-Spec Affinity purification to identify the host proteins interacting with SARS-CoV-2 Orf6 protein and tested a drug that could inhibit a specific Orf6 and host protein interaction. (biomedcentral.com)
Composition2
  • However, the molecular composition and operation of the encoded 'mycobacterial mutasome' - minimally comprising DnaE2 polymerase and ImuA' and ImuB accessory proteins - remain elusive. (janelia.org)
  • Newly formed nascent adhesions gradually grow and change their protein composition to mature into FAs. (life-science-alliance.org)
Human1
  • To test the idea that loss of Rab11 may promote cancer progression, archival human patient tissues were analyzed and 51 out of 70 colon cancer tissues had lower Rab11 protein staining. (sdbonline.org)
Diseases1
  • Functional impairments of Rabs or their accessory proteins are manifested in a range of diseases including neurological disorders and diabetes as well as in infectious diseases. (ximbio.com)
Domain3
  • First is the disruption of enterocyte polarity based on staining of the FERM domain protein Coracle . (sdbonline.org)
  • HECT domain E3 ubiquitin protein li. (gsea-msigdb.org)
  • The functions of these accessory proteins remain largely unresolved since they lack well-defined domain structures. (biomedcentral.com)
Transport2
  • In contrast, loss of the specific subunits of TRAPPII, another known Rab11-GEF, does not cause any defects on the eye development nor the transport of rhabdomere proteins, however, simultaneous loss of TRAPPII and Pcs shows severe defects on eye development. (sdbonline.org)
  • The present study unravels a new molecular system for vesicle-based axonal transport of proteins in male and female flies (Drosophila melanogaster). (sdbonline.org)
Disease1
  • Thompson ja infant botulism in the supraoptic nu-clei, paraventricular nuclei, and accessory nuclei of the original renal disease. (elastizell.com)