• Herein, we present the first evaluation of cationic dendrimers based on 2,2-bis(methylol)propionic acid (bis-MPA) as nonviral vectors for transfection of short interfering RNA (siRNA) in cell cultures. (mdpi.com)
  • Poly(ethylenimine) was the second polymeric transfection agent discovered, after poly-L-lysine. (wikipedia.org)
  • This article evaluations discoveries pushed by combinatorial library synthesis and screening, together with enantioselective esterase and aldolase enzyme fashions, cobalamin binding and peroxidase dendrimers, glycopeptide dendrimer biofilm inhibitors and their X-ray crystal buildings as complexes with lectins, antimicrobial peptide dendrimers lively in opposition to multidrug resistant Gram-negative micro organism, and transfection reagents for siRNA and CRISPR-Cas9 plasmid DNA. (mckessonbio.com)
  • Cationic dendrimers such as poly(propyleneimine) (PPI), poly(amidoamine) (PAMAM), poly(L-Lysine) (PLL) and carbosilane are able to form complexes with siRNA through charge interactions and shielding it from RNases. (polymerfactory.com)
  • mPEG-SCM (Amine PEGylation of Proteins) - PEGylation of lysine residues improves the proteolytic stability of fibronectin while retaining biological activity, Biotechnol. (creativepegworks.com)
  • Ubiquitin on the E2 enzyme is conjugated to lysine residues within the protein of interest, or the N-terminal amino group, by a ubiquitin (E3) ligase. (celeristx.com)
  • Native mass spectrometry imaging of intact proteins and protein complexes in thin tissue sections. (bham.ac.uk)
  • Aiming at learning cooperativity results between amino acids in simply accessible protein fashions, we now have explored the chemistry of peptide dendrimers, which we receive as pure merchandise by solid-phase peptide synthesis utilizing a branching diamino acid resembling lysine at each second or third place in a peptide sequence, adopted by reverse-phase HPLC purification. (mckessonbio.com)
  • A pure form of proteolytic enzyme is used to hydrolyze the protein to produce short peptide sequences. (psyclopeptide.com)
  • mPEG-SCM (Amine PEGylation of Lysine Peptide) - PEG-Like Nanoprobes: Multimodal, Pharmacokinetically and Optically Tunable Nanomaterials, PLoS ONE 9(4): e95406, Text . (creativepegworks.com)
  • Since this is the first study on bis-MPA dendrimers as a potential non-viral carrier for siRNA delivery, the results are relatively promising and more studies are required to understand the system better and potentially discover ways to take measures to improve the existing protein knockdown rate. (polymerfactory.com)
  • An unusual cellular factor potentiates protein-DNA complex assembly between Oct-1 and Vmw65. (mcmaster.ca)
  • Consideration of the external force field in the form of polar water (Fuzzy Oil Drop) and a version modified by the presence of the hydrophobic compounds, FOD-M (FOD-Modified) reveals that the protein folding process is environmentally dependent. (bvsalud.org)
  • Liquid Extraction Surface Analysis for Native Mass Spectrometry: Protein Complexes and Ligand Binding. (bham.ac.uk)
  • Direct Tissue Profiling of Protein Complexes: Toward Native Mass Spectrometry Imaging. (bham.ac.uk)
  • Native Liquid Extraction Surface Analysis Mass Spectrometry: Analysis of Noncovalent Protein Complexes Directly from Dried Substrates. (bham.ac.uk)
  • Additional ubiquitin molecules are conjugated onto the first, forming a polyubiquitinated adduct recognized by the proteasome leading to degradation of the protein of interest. (celeristx.com)
  • Complexes of Lysine Dendrimers of 2nd Generation with Semax and Epithalon Peptides. (iaras.org)
  • There are hundreds of cytokines, generally peptides and glycopeptides), and they have a superbly complex family tree. (barefacedtruth.com)
  • By forming Au-S bonds, self-assembled molecules may trigger monolayer modification on the gold floor and are additional linked with totally different biomolecules by way of varied useful teams resembling -OH, COOH, and NH2. (mckessonbio.com)
  • The study encompassed dendrimers of generation one to four (G1-G4), modified to bear 6-48 amino end-groups, where the G2-G4 proved to be capable of siRNA complexation and protection against RNase-mediated degradation. (mdpi.com)
  • Amino-functional bis-MPA dendrimers of generation 1 to 4 (G1 to G4) have been investigated as a non-viral vector for siRNA delivery in primary neurons, astrocytes, glioma, and glioblastoma cell lines. (polymerfactory.com)
  • G2 to G4 formed charge-interaction complexes with siRNA efficiently with G3 and G4 showing dose-dependent cytotoxicity towards primary neurons while G2 shows no signs of toxicity. (polymerfactory.com)
  • Alternatively, it might also due to a strong charge-interaction between dendrimer and siRNA which inhibits the release of siRNA. (polymerfactory.com)
  • In this study, the Chitosan-Ag (CS-Ag) complex hydrogels were prepared using the physical crosslinking method, which used for controlling postharvest diseases in grape. (preprints.org)
  • During this training, she invented several natural biopolymers based in situ forming hydrogels to promote corneal wound healing. (stanford.edu)
  • In addition, these hydrogels have a distinctive capacity to form in situ and dissolve on-demand via physical or chemical reactions. (biomedcentral.com)
  • Therefore, there is a critical unmet need for a topically applied material that (1) is easily applied and forms in situ, (2) is of sufficient mechanical flexibility to accommodate complex wound contours and volumes, (3) can be easily and atraumatically removed under controlled conditions for definitive surgical care, and (4) is non-toxic. (biomedcentral.com)
  • To improve mechanical properties such as dimensional stability of the resultant textile product a second layer of a carrier material has been used (also previously been referred to in the prior art as a secondary layer and/or support sheet) and is typically glued to the back side of the primary layer to form a laminated textile product with the desired properties. (justia.com)
  • Understanding the functional biology of CSCs - especially the signaling pathways that are involved in their self-renewal mechanisms - is crucial for discovering new forms of treatment. (docksci.com)
  • These challenges include identifying clinically relevant but nonteratogenic forms of the drug, and the mechanisms underlying phocomelia and species specificity. (hindawi.com)
  • Fluorescence microscopy experiment also suggested efficient endo-lysosomal escape with distinct mitochondrial subcellular localisation for all generation dendrimers. (polymerfactory.com)
  • There are considerable data indicating that CSCs could be responsible for complex tumor biology, including initiation, growth, and regeneration. (docksci.com)
  • Among most immunosuppressive cells, TAMs, as crucial drivers of immunosuppressive TME, account for the largest proportion of immune cells in the TME (approximately 50% of tumor mass) [ 9 , 10 ], which possess highly heterogeneity and play a complex regulatory role in tumor immunity and immunotherapy due to helping tumor evade immune surveillance [ 11 ]. (thno.org)
  • Cancer progression is mainly driven by the genetic alterations in complex signaling pathways that leads to the activation of specific oncogenes and inactivation of tumor suppressor genes 9 - 12 . (ntno.org)
  • γ-Glu-AA-AAs can enhance the umaminess of Monosodium Glutamate (MSG) as they can enter the binding pocket of the taste receptor type 1 subunit 3 (T1R3)-MSG complex. (bvsalud.org)
  • Instead, a specific subset of ISGs that includes Parp9 remains activated by the unphosphorylated form of ISGF3 that enhances nDNA damage and repair responses. (regenerativemedicine.net)
  • The cell viability, reactive oxygen species (ROS) levels, ratios of oxidized nicotinamide adenine dinucleotide to its reduced form (NAD + /NADH), apoptosis assays, and cellular uptake of E/PCF-NPs were determined on 4T1 cells. (dovepress.com)
  • In this case, bis-MPA dendrimers came in handy by being more biocompatible and biodegradable at physiologically relevant pH and temperature. (polymerfactory.com)
  • Thus, we report a rare-earth-based P, N-containing complex with a lamellar aggregated structure [Ce(DPA)3] by a coordination reaction between a tailored ligand and cerium(III) nitrate. (edu.au)
  • Textile products used to cover floors (e.g. as carpets) are commonly produced by stitching a yarn onto a first material to form a yarn bearing layer having tufts of yarn protruding from a top surface. (justia.com)
  • PDF is the official format for papers published in both, html and pdf forms. (mdpi.com)
  • Indeed Dr Frances Kelsey published several papers on improving drug testing and safety, which form the basis of drug testing carried out today [ 5 , 6 ]. (hindawi.com)
  • Lipofectamine2000 showed toxicity at lowest dose (1 microg/mL), whereas FuGENE6 and low molecular weight poly-L-lysine (PLL) showed the least toxicity. (nih.gov)
  • Poly(ethylenimine) was the second polymeric transfection agent discovered, after poly-L-lysine. (wikipedia.org)
  • Representatives in this category are synthetic polymers, such as poly-L-lysine, poly-L-ornithine, linear and branched polyethyleneimine (PEI), diethylaminoethyl- Dextran, polyamide-amine dendrimer (PAMAM) and polydimethylaminoethyl methacrylate (PDMAEMA). (adpostman.com)
  • Different classes of TAs were used, such as polycationic amines, dendrimers, and lipid-based agents. (nih.gov)
  • In particular, the combination of DNA with a hydrophobic polymer chain resulted in amphiphilic species that formed supramolecular architectures such as micelles or vehicles. (bocsci.com)
  • Liposomes are composed of phospholipids, which are easy to form a closed lipid bilayer in an aqueous solvent, thereby forming nano-scale particles. (adpostman.com)
  • Linear polyethyleneimine is able to form cryogels upon freezing and subsequent thawing of its aqueous solutions. (wikipedia.org)
  • By complexing ferumoxides or superparamagnetic iron oxide (SPIO) to transfection agents (TAs), it is possible to magnetically label mammalian cells. (nih.gov)
  • Arp2-3 complex binds WASP PROTEIN and existing ACTIN FILAMENTS, and it nucleates the formation of new branch point filaments. (nih.gov)
  • HN - 2006 BX - Arp2-3 Complex MH - Actin-Related Protein 3 UI - D051378 MN - D5.750.78.730.246.750 MN - D12.776.220.525.246.750 MS - A component of the Arp2-3 complex that is related in sequence and structure to ACTIN and that binds ATP. (nih.gov)
  • Osmotic swelling results and bursts the vesicle releasing the polymer-DNA complex (polyplex) into the cytoplasm. (wikipedia.org)
  • In recent years, DNA-polymer conjugates have emerged as versatile building blocks that provided enhanced stability to complex DNA nanostructures or facilitated, e.g., the precise organization of polymeric structures by DNA. (bocsci.com)
  • Due to the instability of miRNAs and their complex environment, including degradation by nucleases in vivo, the safety and effectiveness of miRNA delivery has become the focus of recent attention. (biomedcentral.com)
  • This lipid complex protects the genetic material from degradation and is delivered in mammalian cells. (adpostman.com)
  • Once internalized, a single bacterium that escapes into the host cell cytoplasm can rapidly replicate and progress to form a biofilm-like intracellular bacterial community (IBC). (justia.com)
  • The miRISC complex containing a mature miRNA strand can bind to the 3′-UTR of target gene mRNA. (biomedcentral.com)
  • Polymers can be combined with nucleic acids to form multimeric complexes at physiological pH to facilitate gene delivery. (adpostman.com)
  • Type 1 pili are anchored in the bacterial outer membrane and are largely composed of repeating FimA protein subunits which form a helically wound cylinder that comprises the thick pilus rod. (justia.com)
  • HN - 2006(1981) BX - Cofilins MH - Actin-Related Protein 2 UI - D051377 MN - D5.750.78.730.246.500 MN - D12.776.220.525.246.500 MS - A PROFILIN binding domain protein that is part of the Arp2-3 complex. (nih.gov)
  • HN - 2006(1998) MH - Actin-Related Protein 2-3 Complex UI - D051376 MN - D5.750.78.730.246 MN - D12.776.220.525.246 MS - A complex of seven proteins including ARP2 PROTEIN and ARP3 PROTEIN that plays an essential role in maintenance and assembly of the CYTOSKELETON. (nih.gov)
  • To confirm K244 as the site of cleavage, we mutated lysine 244 to alanine (K244A) in GSDMB and tested whether the mutant protein can be cleaved by HDM. (authorea.com)
  • There are hundreds of cytokines, generally peptides and glycopeptides), and they have a superbly complex family tree. (barefacedtruth.com)
  • Dendrimeric Antigens (DeAns) consist of dendrimers decorated with multiple units of drug antigenic determinants. (mdpi.com)
  • Using the sequence-dependent unique recognition properties of DNA base pairs and negatively charged sugar-phosphate backbones, our R&D team can utilize multiple forms of DNA as templates and different nanostructures such as nanowires, nanorings, nanorods, nanoprticle superlattice, nanoclusters, core-shell, nano-snowman, are perfectly synthesized by bottom-up techniques. (bocsci.com)
  • There has been no systematic study comparing TAs complexed to SPIO as far as cell labeling efficiency and viability. (nih.gov)
  • This study investigates the toxicity and labeling efficiency at various doses of FEs complexed to different TAs in mammalian cells. (nih.gov)
  • In the present study, we constructed a HER2 IgY-SWNT complex and demonstrated its dual functionality for both detection and selective destruction of cancer cells in an in vitro model consisting of HER2-expressing SK-BR-3 cells and HER2-negative MCF-7 cells. (biomedcentral.com)
  • This result indicates that GSDMB was cleaved immediately after the lysine residue at position 244 (K244). (authorea.com)
  • Diagnostic imaging with hydrophilic contrast agents enables the detection of pathologic hyperpermeability in the form of abnormal contrast enhancement of the diseased tissue interstitium. (biomedcentral.com)
  • Concentrations of 25 to 50 microg/mL of FEs complexed to TAs in media resulted in sufficient endocytosis of the SPIO into endosomes to detect cells on cellular magnetic resonance imaging. (nih.gov)
  • NIR irradiation selectively destroyed the complex-targeted breast cancer cells without harming receptor-free cells. (biomedcentral.com)
  • We have demonstrated that the HER2 IgY-SWNT complex specifically targeted HER2-expressing SK-BR-3 cells but not receptor-negative MCF-7 cells. (biomedcentral.com)
  • The complex can be potentially used for both detection and selective photothermal ablation of receptor-positive breast cancer cells without the need of internalization by the cells. (biomedcentral.com)
  • The mechanisms of these compounds include modifing DNA bases, intercalating between bases, or forming crosslinks in DNA. (creativebiolabs.net)
  • It is also used as flocculating agent with silica sols and as a chelating agent with the ability to complex metal ions such as zinc and zirconium. (wikipedia.org)