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LuminolLuminescent MeasurementsLuminescenceAcridinesFlow Injection AnalysisIsocoumarinsHorseradish PeroxidasePyridazinesPeroxidaseFree RadicalsSuperoxidesEncyclopedias as TopicFerricyanidesCyclizationHydrogen PeroxideEnterohemorrhagic Escherichia coliEscherichia coli O157RabbitsEscherichia coli InfectionsEscherichia coli ProteinsCommerceZoonoses
ChemiluminescenceChemiluminescentReagentDetectionHydrogen peroxideAssayReactionH2O2SodiumEmit lightEnhancerForensicOxidationIntensityInvestigatorsAptamerNanoparticlesElectrochemicalOxideSensitiveSolutionGlowCompoundPowderInsolubleExhibitChemicalLightBloodAmountMolecularInteractionsSpecificConditionsFreeShownPersonEnvironmentCreate
Chemiluminescence12
  • Luminol (C8H7N3O2) is a chemical that exhibits chemiluminescence, with a blue glow, when mixed with an appropriate oxidizing agent. (wikipedia.org)
  • The exact mechanism of luminol chemiluminescence is a complex multi-step reaction, especially in aqueous conditions. (wikipedia.org)
  • The study based on the chemiluminescence (CL) reaction of potassium ferricyanide and luminol in sodium hydroxide medium, enrofloxacin (ENRO) could dramatically enhance CL intensities and incorporated with matrix solid-phase dispersion (MSPD) technique (Florisil used as dispersant, dichloromethane eluted the target compounds). (hindawi.com)
  • In our previous study, we examined luminal chemiluminescence (CL) behavior in a ternary mixed solvent solution of water-acetonitrile-ethyl acetate, and the mixed solution was found to enhance luminol CL in time and intensity under certain conditions [12]. (scirp.org)
  • Herein, the role of gal-3 in regulating S. aureus phagocytosis and S. aureus -induced intracellular ROS was analyzed by imaging flow cytometry and luminol-based chemiluminescence, respectively. (lu.se)
  • induced intracellular ROS was analyzed by imaging flow cytometry and luminol-based chemiluminescence, respectively. (lu.se)
  • This new method is based on luminol- peroxide chemiluminescence (CL) assays for the detection of cobalt( II ) ions in vitamin B 12 molecules. (rsc.org)
  • It is a non-isotopic, luminol-based chemiluminescence substrate for Horseradish Peroxidase (HRP), designed for the chemiluminescent detection of immobilized proteins conjugated with horseradish peroxidase (HRP). (sigmaaldrich.com)
  • This research investigated the applicability of a luminol-based chemiluminescence assay to monitor transport of horseradish peroxidase (HRP) enzyme in saturated porous media. (k-state.edu)
  • The chemiluminescence assay was optimized by varying solution conditions such as the concentration of luminol, p-iodophenol, hydrogen peroxide, ionic strength and pH. (k-state.edu)
  • As a raw material supplier of chemiluminescence reagent , Desheng has a complete range of product types, which can supply different experimental needs, and products such as Luminol, Isoluminol , luminol sodium salt ( 20666-12-0) , etc. with stable luminescence, without any failure. (hbdsbio.com)
  • A novel and rapid (45min), quantitative chemiluminescence-based, surface immunoassay is reported for the quantitative detection of progesterone and œstriol in artificial saliva. (worktribe.com)
Chemiluminescent10
  • Unlike detection systems based on fluorescence, chemiluminescent methods do not require external light sources for excitation energy. (the-scientist.com)
  • Commercially available chemiluminescent HRP substrates include luminol- and acridan-based formulations. (the-scientist.com)
  • Acridan- and 1,2-dioxetane-based substrates are available for the chemiluminescent detection of AP. (the-scientist.com)
  • The FastGene Western ECL Kit is an enhanced chemiluminescent (ECL) substrate based on luminol. (bulldog-bio.com)
  • This long chemiluminescent signal duration makes it possible to detect signals on both digital and film-based imaging systems without any loss in signal intensity. (bulldog-bio.com)
  • Mix the luminol solution and peroxide solution in a 1:1 ratio, and thoroughly agitate the chemiluminescent substrate solution well for preparing the 0.1 ml of solution / cm 2 of membrane. (bulldog-bio.com)
  • Two sensitive luminol-based enhanced chemiluminescent substrates. (proteinark.com)
  • AbFrontier WESTSAVE UpTM (Western Blotting Substrate) is enhanced luminol-based chemiluminescent substrate for the non-radioactive detection of Horseradish Peroxidase(HRP) labeled antibodies. (virologyhighlights.com)
  • Using luminol or a derivative of luminol as a substrate for enzymatic activity by HRP, you get a chemiluminescent reaction. (sepmag.eu)
  • T-Pro ECL Kit a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots. (tprobio.com)
Reagent3
  • In this study, we examined CL behavior with four kinds of mixed solutions of water only, water-acetonitrile, and ternary mixed solutions of water-acetonitrile-ethyl acetate for peroxyoxalate reagent, instead of luminol reagent [13] [14], in the presence of Eosin Y as a model fluorescence compound. (scirp.org)
  • Lightning Luminol is a water-based spray reagent used for the detection of trace amounts of blood. (hecdistribution.com)
  • A reagent containing luminogenic substrates (a luminol derivative and a peracid salt) and an electron transfer agent, is added to the wells. (cdc.gov)
Detection18
  • Under the optimal conditions obtained by a face-centered central composite design (FCCD), the PMT-based detection of the BPE-ECL aptasensor exhibit a linear response over a wide dynamic range from 5 to 150ngmL-1, with a detection limit of 0.01ngmL-1. (unboundmedicine.com)
  • Additionally, smartphone-based detection shows a linear relationship between the ECL image gray value and the logarithmic concentration of the AFM1 target over a range of 10-200ngmL-1, with a detection limit of 0.05ngmL-1. (unboundmedicine.com)
  • This smartphone-based detection opens a new horizon for bioanalysis that does not require a trained technician to operate and is a promising technology for point-of-care testing. (unboundmedicine.com)
  • The amount of catalyst necessary to cause the reaction is very small relative to the amount of luminol, allowing detection of even trace amounts of blood. (wikipedia.org)
  • Electrochemiluminescence and electrochemical dual-mode detection of BACE1 activity based on the assembly of peptide and luminol co-functionalized silver nanoparticles induced by cucurbit[8]uril. (bvsalud.org)
  • Stainless steel electrode for sensitive luminol electrochemiluminescence detection of H 2 O 2 , glucose, and glucose oxidase activity. (edu.et)
  • Fuad A. B., Shimeles A . K . , Yong W., Haijuan Li, Ping W., Jin Y. D. Plasmon-Boosted Cu-Doped TiO 2 Oxygen Vacancy-Rich Luminol Electrochemiluminescence for Highly Sensitive Detection of Alkaline Phosphatase, Analytical Chemistry , 2021 , 93, 15183-15191. (edu.et)
  • Ratiometric Electrochemiluminescent/Electrochemical Strategy for Sensitive Detection of MicroRNA Based on Duplex-Specific Nuclease and Multilayer Circuit of Catalytic Hairpin Assembly. (scienceopen.com)
  • 3. The brightness in the detection environment is too strong, and Luminol fluorescence cannot be distinguished or even difficult to emit light. (hbdsbio.com)
  • Detection of anti-HCV by SIA methodology is based upon traditional Western and dot blotting techniques, in which specific immunogens (i.e. antigenic polyproteins) encoded by the HCV genome are immobilized onto a membrane support. (cdc.gov)
  • Yuan, R.*. Sandwiched Electrochemiluminescent Peptide Biosensor for the Detection of Prognostic Indicator in Early-Stage Cancer Based on Hollow, Magnetic, and Self-Enhanced Nanosheets. (yuanruogroup.com)
  • Yuan, R.*. An electrochemical peptide cleavage-based biosensor for prostate specific antigen detection via host-guest interaction between ferrocene and beta-cyclodextrin. (yuanruogroup.com)
  • Yuan, R.*. A ferrocene-switched electrochemiluminescence "off-on'' strategy for the sensitive detection of cardiac troponin I based on target transduction and a DNA walking machine. (yuanruogroup.com)
  • Yuan, R*. Enzyme-Free Electrochemical Immunosensor Based on Host-Guest Nanonets Catalyzing Amplification for Procalcitonin Detection. (yuanruogroup.com)
  • Chai, Y.Q.*.Electrochemical Peptide Biosensor Based on in Situ Silver Deposition for Detection of Prostate Specific Antigen. (yuanruogroup.com)
  • Chai, Y.Q.*. Sensitive electrochemiluminescence detection for CA15-3 based on immobilizing luminol on dendrimer functionalized ZnO nanorods.Biosens. (yuanruogroup.com)
  • Yuan, R.*. A label-free electrochemical aptasensor based on the catalysis of manganese porphyrins for detection of thrombin. (yuanruogroup.com)
  • Yuan, R*. Ultrasensitive simultaneous detection of four biomarkers based on hybridization chain reaction and biotin-streptavidin signal amplification strategy.Biosens. (yuanruogroup.com)
Hydrogen peroxide2
Assay4
  • Using a newly developed cis-parinaric acid-based procedure to assay site specific oxidative stress in membrane phospholipids, we found that phenol at subtoxic concentrations. (cdc.gov)
  • DNAzyme-Triggered Visual and Ratiometric Electrochemiluminescence Dual-Readout Assay for Pb(II) Based on an Assembled Paper Device. (scienceopen.com)
  • Herein, a dual-mode lab-on-paper platform is presented, which integrates distance-based visualized readout with ratiometric electrochemiluminescence (ECL) assay in one device. (scienceopen.com)
  • The Western blot assay uses gel electrophoresis (SDS-PAGE or native PAGE) to separate proteins based on their molecular weight. (kangle.skin)
Reaction3
  • and isolates were biotyped, serotyped, tested by polymerase chain reaction (PCR), and genotyped by repetitive-element sequence-based PCR (Rep-PCR). (cdc.gov)
  • The distance-based measurement is based on a brown visualized strip generated from the oxidation reaction of 3,3'-diaminobenzidine in the presence of H2O2 initiated by horseradish peroxidase (HRP). (scienceopen.com)
  • 5. If antioxidants are used and the reaction mechanism of luminol is suppressed, failure will occur. (hbdsbio.com)
H2O22
  • the resulting ECL of luminol and H2O2 at the anodic poles is monitored using a photomultiplier tube (PMT) or smartphone, and the images are analyzed using ImageJ software. (unboundmedicine.com)
  • Concretely, upon addition of Pb2+ into the fabricated device, cleaved oligonucleotide fragments connected with HRP functionalized Au nanocubes could permeate through the cellulose on account of their size that is smaller than paper pores, quench the ECL signal of the CdS quantum dots because of resonance energy transfer, and synchronously boost the ECL intensity generated from luminol by catalyzing H2O2. (scienceopen.com)
Sodium1
  • Reduction of the nitro group to an amino group with sodium dithionite (Na2S2O4), via a transient hydroxylamine intermediate, produces luminol. (wikipedia.org)
Emit light1
  • A molecule known as hemin then activates the DNAzyme, which transforms another molecule called luminol, making it emit light. (foresight.org)
Enhancer1
  • Commercially sold luminol solutions will often have enhancer molecules to create a greater signal. (sepmag.eu)
Forensic2
  • Forensic investigators use luminol to detect trace amounts of blood at crime scenes, as it reacts with the iron in hemoglobin. (wikipedia.org)
  • Luminol is a forensic compound used to test for blood stains at crime scenes. (doctorshealthpress.com)
Oxidation2
  • The HRP in the bound conjugate catalyzes the oxidation of the luminol derivative, producing light. (cdc.gov)
  • Amplified Thrombin Aptasensor Based on Alkaline Phosphatase and Hemin/G-Quadruplex-Catalyzed Oxidation of 1-Naphthol.ACS Appl. (yuanruogroup.com)
Intensity1
  • We found that a strong CL signal was emitted when a trace amount of ENRO was added to luminol-potassium ferricyanide solution, and its intensity was strongly dependent on the ENRO concentration. (hindawi.com)
Investigators4
  • Crime scene investigators use luminol to find traces of blood, even if someone has cleaned or removed it. (wikipedia.org)
  • A blacklight photo of the mop shows blood that showed up in mop strokes on the floor after crime scene investigators applied the chemical luminol. (news4jax.com)
  • Based on the evidence collected in the case, investigators are considering whether Kessler dismembered Cummings. (news4jax.com)
  • Luminol, as a 'member' of luminescent reagents, is often used in solving cases based on its performance, and criminal investigators can also discover clues based on it, such as whether blood has been left at the scene. (hbdsbio.com)
Aptamer1
  • Luminol-functionalized silver nanoparticle-decorated graphene oxide (GO-L-AgNPs) participates in π-π interactions with the unpaired bases of the immobilized aptamer (Apt-GMNPs-GO-L-AgNPs). (unboundmedicine.com)
Nanoparticles1
  • Yuan, R*. Au nanoparticles decorated C-60 nanoparticle-based label-free electrochemiluminesence aptasensor via a novel "on-off-on" switch system. (yuanruogroup.com)
Electrochemical2
  • The obtained (CB[8]-P2-AgNPs- luminol )n aggregates with both ECL and electrochemical activity, used as the dual-mode signal probe, could be captured to the N-terminal of P1 through CB[8]. (bvsalud.org)
  • Yuan, R*. 3,4,9,10-Perylenetetracarboxylic acid/o-phenylenediamine nanomaterials as novel redox probes for electrochemical aptasensor systems based on an Fe3O4 magnetic bead as a nonenzymatic catalyst. (yuanruogroup.com)
Oxide1
  • In, I. ChemiluminescenceQuenching of Luminol-Functionalized Chemically ReducedGraphene Oxide through Noncovalent Interaction. (edu.pl)
Sensitive1
  • Based on this result, a rapid, sensitive, and inexpensive CL method with MSPD technique was proposed to determine the ENRO content. (hindawi.com)
Solution1
Glow2
  • When luminol is sprayed evenly across an area, trace amounts of an activating oxidant make the luminol emit a blue glow that can be seen in a darkened room. (wikipedia.org)
  • Interestingly, artemisinin is one of the key elements in the proposed treatment approach, since artemisinin has been found to enhance the glow luminol gives off when it encounters the hemoglobin in red blood cells. (doctorshealthpress.com)
Compound1
  • The compound was first synthesized in Germany in 1902, but was not named "luminol" until 1934. (wikipedia.org)
Powder1
  • Nowadays, there are many manufacturers producing Luminol reagents in the market, and their appearance is mostly luminol powder for sale . (hbdsbio.com)
Insoluble1
  • Luminol is a white-to-pale-yellow crystalline solid that is soluble in most polar organic solvents, but insoluble in water. (wikipedia.org)
Exhibit1
Chemical1
  • The most common and large-scale approach to control leafcutter ants is based on chemical control (Della Lucia et al. (springer.com)
Light1
Blood1
  • What the luminol shows is that there was blood that could have been, that was cleaned up," Chrabot said. (news4jax.com)
Amount1
  • The CL signal produced by the emission of photons from luminol was directly proportional to the amount of analyte. (ijbs.com)
Molecular1
  • One of the authors, Itamar Willner, previously built an enzyme-based molecular computer for potential use inside the human body to assist with drug delivery. (foresight.org)
Interactions1
  • What the Washington researchers aim to do is devise a therapy based around these interactions. (doctorshealthpress.com)
Specific2
  • On the contrary, a ratiometric ECL technique was employed for accurate analysis based on the specific biorecognition between Pb2+-dependent DNAzymes and targets. (scienceopen.com)
  • One such approach is to confirm the properties of target-specific antibodies, including cell lysates Ö cell lines they are known to contain or not contain the target of interest based on genomic and proteomic studies. (kangle.skin)
Conditions1
  • Luminol is first deprotonated in basic conditions, then oxidized to the anionic radical. (wikipedia.org)
Free1
Shown1
Person1
  • Japanese adventures tended to be in first person, and use menu based interfaces, they were also heavily represented in the uncensored PC gaming space which lead to numerous 18+ erotic titles. (neogaf.com)
Environment1
  • Recent reports have also emphasized that farm animals and their environment pose a zoonotic risk for humans, based on outbreaks of E. coli O157:H7 infection among farm visitors ( 4 ). (cdc.gov)
Create1
  • Since the main components, the ALA amino acid, luminol, and artemisinin, are approved for human use individually, it is hoped that the combination will not create health hazards either. (doctorshealthpress.com)