Luciferase genes reporter. Medical search. Frequent questions

Q1：Optimum reaction temperature for dual luciferase reporter gene? (yeasenbiotech.com)
Q2：How to choose a dual luciferase reporter gene vector? (yeasenbiotech.com)
The miRNA-mRNA interactions were verified by dual-luciferase reporter gene assays and experiments using mimics miRNA or their inhibitors. (frontiersin.org)
Dual-luciferase reporter gene assays demonstrated Arhgap11a as the target of miR-211-5p. (frontiersin.org)

Gene ID: 5925, OMIM 614041 ) gene, which encodes a tumor suppressor protein. (molvis.org)
The Metridia longa secreted luciferase gene encodes a 24 kDa protein containing an N-terminal secretory signal peptide of 17 amino acid residues. (wikipedia.org)
The payload is also DNA, which encodes both a reporter gene and the CRISPR/Cas9 system. (futurity.org)
This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. (cancerindex.org)
Silencing the YBX1 gene (which encodes the YB-1 protein) by small interfering RNA (siRNA) was performed in MDA-MB-231 and Hs578T breast cancer cell lines, followed by phenotypic assays including cell migration and invasion assays. (biomedcentral.com)
Global gene expression profiling in the YBX1 silenced MDA-MB-231 cells identified differential expression of several genes, including CORO1C (which encodes for an actin binding protein, coronin-1C) as a potential downstream target of YB-1. (biomedcentral.com)
One of the well-known reporter genes is one that encodes for a protein called green fluorescent protein - or GFP. (jove.com)
mutations high frequency of IDH1/2 mutations in oligodendrogliomas, astrocytomas and in alteRations in the RB1 pathway in The TET2 gene encodes the -KG- secondary glioblastomas derived thereof low-gRade diffuse gliomas lacking dependent enzyme that catalyses suggests that these tumours share a common genetic alteRations the conversion of 5-methylcytosine to common progenitor cell population. (who.int)

Firefly luciferase and Renilla luciferase detection systems are mostly used in traditional reporter gene detection systems, but the short half-life of the luminescent signal and poor stability, etc., affect the experimental results and use and cause inconvenience. (yeasenbiotech.com)
A：1) Firefly luciferase is recommended to choose pGL-3 or pGL-4 or a self-constructed vector. (yeasenbiotech.com)
Q3：What is the appropriate ratio of firefly luciferase readings to Renilla luciferase readings? (yeasenbiotech.com)
A：It is recommended that the readings of control firefly and Renilla luciferase are close, or that the reading of Renilla luciferase is slightly higher than that of firefly luciferase. (yeasenbiotech.com)
During the actual experiment, due to the inconsistent quality of the plasmids extracted from firefly luciferase and Renilla luciferase, and the different regulatory capabilities of the target regulatory elements to be detected, we recommend pre-experiment optimization of firefly luciferase. (yeasenbiotech.com)
One well-studied luciferase is that of the Photinini firefly Photinus pyralis, which has an optimum pH of 7.8. (wikipedia.org)
ATP Cell Viability Luciferase Assay A highly sensitive firefly luciferase cell based assay for quantifying ATP in cell cultures used to measure cell viability. (sigmaaldrich.com)
In tests, the BV was loaded with green fluorescent proteins or firefly luciferase. (futurity.org)
The most common types of luciferase found in Addgene's collection are Firefly luciferase (Fluc), Renilla luciferase (Rluc), and NanoLuc® Luciferase (Nluc). (addgene.org)
Luc2 is the second-generation version of Firefly luciferase that has been codon optimized for expression in mammalian systems. (addgene.org)
Like Firefly luciferase, Click-beetle red luciferase has also been codon optimized ( CBR2opt ) for mammalian expression systems. (addgene.org)
Firefly luciferase is considerably brighter than Renilla luciferase, but is more susceptible to enzyme inhibition. (addgene.org)
Both Firefly luciferase and Renilla luciferase have short protein half-lives, making them useful as transcriptional reporters. (addgene.org)
Firefly and Renilla luciferase are less-than-ideal protein tags due to their large size. (addgene.org)
A modified firefly luciferase engineered to use the red-shifted substrate Akalumine with significantly brighter signal and emission in the near-infrared range, enabling enhanced bioluminescence imaging in vivo . (addgene.org)
A set of three sensors that are increasingly structurally destabilized versions of Firefly luciferase which serves as a convenient toolkit to assess the proteostasis status in a wide range of experimental systems. (addgene.org)

In this organism, the luciferase (Renilla-luciferin 2-monooxygenase) is closely associated with a luciferin-binding protein as well as a green fluorescent protein (GFP). (wikipedia.org)
Light emission in some bioluminescent bacteria utilizes 'antenna' such as lumazine protein to accept the energy from the primary excited state on the luciferase, resulting in an excited lulnazine chromophore which emits light that is of a shorter wavelength (more blue), while in others use a yellow fluorescent protein (YFP) with flavin mononucleotide (FMN) as the chromophore and emits light that is red-shifted relative to that from luciferase. (wikipedia.org)
Dinoflagellate luciferase is a multi-domain eukaryote protein, consisting of an N-terminal domain, and three catalytic domains, each of which preceded by a helical bundle domain. (wikipedia.org)
It regulates Sp7 protein expression and induces expression of major bone matrix protein genes, such as Col1a1, Spp1, Ibsp, Bglap2, and Fn1 ( 7 , 8 ). (frontiersin.org)
Recombinant Lucia Luciferase Protein is a positive control for luciferase reporter assays. (invivogen.com)
A dilution series of the recombinant Lucia luciferase protein can also be used to determine the linear range of the assay. (invivogen.com)
Activity of recombinant Lucia luciferase protein determined by measuring Relative Light Units (RLU) in a luminometer using QUANTI-Luc™ . (invivogen.com)
Thus, it has evolved a protection mechanism against heat stress by increasing the expression of the gene coding for heat shock protein (HSP) 70 under elevated temperatures. (biomedcentral.com)
What does this gene/protein do? (cancerindex.org)
NanoLuc® Luciferase is small, bright, and a secreted version of the protein is available - these attributes have made it popular with scientists since its release. (addgene.org)
This compound markedly decreased the hypoxia-induced accumulation of HIF-1α protein dose-dependently, whereas it did not affect the expressions of HIF-1β and topoisomerase-I (topo‑I). Furthermore, celastrol prevented hypoxia-induced expression of HIF-1 target genes for vascular endothelial growth factor (VEGF) and erythropoietin (EPO). (spandidos-publications.com)
Recent research has shed light on a potential biomarker for early metastasis, namely Y-box binding protein-1 (YB-1) encoded by the YBX1 gene. (biomedcentral.com)
The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. (cancerindex.org)
A reporter gene codes for a protein that can be tracked, such as a protein with a known enzymatic activity or one that is fluorescent. (jove.com)
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. (jove.com)
Thus, reporter genes "report" the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell. (jove.com)
Commonly used reporter genes are - GFP (green fluorescent protein gene), lacZ (β- galactosidase gene), RFP (red fluorescent protein gene), and Luc (luciferase gene). (jove.com)
Luciferase reporter assays demonstrated that the Adcy1 promoter is selectively activated by neuronal PAS-domain protein 2 (NPAS2)/BMAL1. (jneurosci.org)

Other luciferases in our collection include Gaussia luciferase (Gluc) and the red and green Click-beetle luciferases (CBRluc and CBGluc, respectively). (addgene.org)
Gaussia luciferase is secreted and more stable, making time course experiments possible, but its short luminescence half-life makes it too dim for many experimental systems. (addgene.org)
A Gaussia luciferase reporter assay was used to determine if CORO1C is a putative YB-1 downstream target. (biomedcentral.com)

Y. Nakajima and Y. Ohmiya, Bioluminescence assays: multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay. (atto.co.jp)

Multiple miRNA-mRNA prediction databases were searched by differentially expressed genes (DEGs) to obtain pairs of a miRNA-DEG regulatory network. (frontiersin.org)
Furthermore, based on differentially expressed genes and WGCNA analyses, we found that the hypoxia signaling pathway was enriched under heat shock conditions. (biomedcentral.com)
Overexpression and silencing of YBX1 were performed to assess the expression of CORO1C , one of the differentially regulated genes from the transcriptomic analysis. (biomedcentral.com)
RNA sequencing identified differentially expressed genes (DEGs) post RBM39 knockdown. (bvsalud.org)

For technology of reporter plasmids, the 39 UTRs of human CASP3 and seven were amplified working with the oligonucleotides NotIhCASP3-3UTRf, XhoIhCASP3-3UTRr, NotIhCASP73UTRf and XhoIhCASP7-3UTRr (table S3). (signsin1dayinc.com)
Endotoxin-cost-free reporter plasmids (pTKGhCASP3 and pTKGhCASP7) were being developed for transfection employing NucleoBond Xtra Midi Furthermore EF (Macherey-Nagel GmbH & Co. KG). (signsin1dayinc.com)
The reporter plasmids pTKGhCASP3 or pTKGhCASP7 were cotransfected with Pre-miR miRNA Precursors hsa-enable-7e or hsamiR-29a (Lifetime Technologies), respectively. (signsin1dayinc.com)
Luciferase-containing plasmids are commonly used to investigate the effect of regulatory elements, such as promoters, enhancers and untranslated regions, or the effect of mutations of these regulatory elements on gene expression. (addgene.org)
Empty backbones for inserting regulatory elements before or after the luciferase ORF can be found in a table below, as well as plasmids that already contain a region of interest before or after luciferase . (addgene.org)

The Yeasen detection system interacts with luciferase, and the luminescence quenching effect of luciferin is comparable to that of the P* brand. (yeasenbiotech.com)
The ATP Cell Viability Luciferase Assay is a flash-type luminescence assay designed for individual sample detection by using a luminometer in a single sample format or a luminometer with an injector in 96-well plate format. (sigmaaldrich.com)
Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. (jove.com)
H. Hoshino, Y. Nakajima and Y. Ohmiya, Luciferase-YFP fusion tag with enhanced emission for single-cell luminescence imaging. (atto.co.jp)

The long-term undifferentiation of molluscan sex chromosomes is potentially sustained by the unexpected intertwined regulation of reversible sex-biased genes, together with the lack of sexual dimorphism and occasional sex chromosome turnover. (nature.com)
The pleiotropic constraint of regulation of reversible sex-biased genes is widely present in ancient homomorphic sex chromosomes and might be resolved in heteromorphic sex chromosomes through gene duplication followed by subfunctionalization. (nature.com)
The regulation of HSP70 by HSF1 was preliminarily confirmed by binding site predictions and by a dual luciferase assay. (biomedcentral.com)
this association is primarily based on the HIF-1-mediated regulation of genes that play pivotal roles in the central features of cancer pathogenesis such as angiogenesis, invasion, metastasis and anti-apoptosis. (spandidos-publications.com)
In the ever-evolving field of molecular biology, scientists are continually exploring the intricate mechanisms that govern gene expression and regulation. (environmental-expert.com)
In order to study the regulation or IRE1 branch of the UPR, and to identify the ER-stress-modulating compounds, a human luciferase reporter cell line (XBP1-NLuc) was created in this work. (helsinki.fi)
Our study demonstrates a circadian rhythm of contrast sensitivity that peaks during the daytime, and that its regulation involves interactions of D4Rs, the clock gene Npas2 , and the clock-controlled gene adenylyl cyclase 1 ( Adcy1 ) in a subset of retinal ganglion cells (RGCs). (jneurosci.org)

2) Renilla luciferase is recommended to select pRL-TK or pGL-4 vector or construct the corresponding vector by yourself. (yeasenbiotech.com)
Co-transfection amount of plasmid and Renilla luciferase plasmid. (yeasenbiotech.com)
Background Renilla Luciferase reporter gene (rLuc) GL4. (biotech-angels.com)

Nucleofection was carried out employing 561056106 HeLa making use of .9.eight mg reporter plasmid (pTK-Gluc derivatives, NEB GmbH), 100200 ng normalisation plasmid (pTK-Cluc, NEB GmbH) and MiRNA expression in contaminated human monocytes was learned using the miRCURY LNATM miRNA Array package v. ten. (signsin1dayinc.com)
Scientists have developed a wide range of luciferase-based plasmid systems for many different applications. (addgene.org)

These data suggest that Sf accounts for most of the ARE-activated transcriptional induction of antioxidant genes by broccoli. (usda.gov)
Similar corresponding luciferase mRNA levels indicate translational rather than transcriptional alterations. (uzh.ch)

In view of the shortcomings of traditional flash type luciferase, Sheng Bio has developed a new generation of glow type luciferase detection system with more convenient operation, longer half-life and better stability through painstaking research, suitable for samples such as mammalian cells, protoplasts, etc. (yeasenbiotech.com)
Other tumor imaging's techniques that rely upon the detection of gene expression (reporter) in the body of the animals have been developed [16] which made a significant impact on both the versatility and the specificity of tumor imaging of animals. (biotech-angels.com)
In addition to TR reporter cells, this kit provides two optimized media for use during cell culture and in diluting the user's test samples, a reference agonist, luciferase detection reagent, and a cell culture-ready assay plate. (thomassci.com)

An MC3T3-E1-based microarray dataset (accessioned as GSE46400) downloaded from the Gene Expression Omnibus included MC3T3-E1 cells with or without 14-day osteoblast differentiation osteoblast induction. (frontiersin.org)
The Arhgap11a, H2afx, Chek2, Dlgap5, and Chek1 were hub genes downregulated in MC3T3-E1 cells after osteogenic differentiation, verified by RT-qPCR results. (frontiersin.org)
Luciferase reporter assay was performed to verify that miR-455 directly binds to the 3′-noncoding region (UTR) of RAB18 mRNA in Huh7 cells. (hindawi.com)
Cells will be labeled with luciferase or other reporter genes. (stanford.edu)
thus its infectivity can be quantified by luciferase activity in virus-infected cells ( 5 - 7 ). (cdc.gov)
MDCK cells were infected with serially diluted pseudovirus stock, and the infectivity reflected by the relative luciferase activity (RLA) was determined as the median (50%) tissue culture infective dose, according to the method of Reed and Muench. (cdc.gov)
Gene expression profiling using Affymetrix GeneChip® Human Transcriptome 2.0 array was subsequently carried out in YB-1 silenced MDA-MB-231 cells. (biomedcentral.com)
While knockdown of YBX1 gene decreased CORO1C gene expression, the opposite effects were seen in YB-1 overexpressing cells. (biomedcentral.com)
Cells co-transfected with an estrogen response element (ERE)-driven luciferase (Luc) reporter gene and an ERalpha- or ERbeta-expression vector were exposed to graded concentrations of ellagic acid. (nutrimedical.com)
INDIGO's panel of TR reporter assays utilizes non-human mammalian cells engineered to express human thyroid hormone receptors alpha (NR1A1) and beta (NR1A2), ligand-dependent transcription factors commonly referred to as TRα and TRβ. (thomassci.com)
INDIGO's TR reporter cells include the luciferase reporter gene functionally linked to a responsive promoter. (thomassci.com)
Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in TRα or TRβ activity. (thomassci.com)
TR reporter cells are prepared using INDIGO's proprietary CryoMite™ process. (thomassci.com)
This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. (thomassci.com)
There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or the pre-incubation of reporter cells prior to assay setup. (thomassci.com)
The pattern and timing of gene expression can be determined by creating recombinant DNA with a reporter gene under the control of a cis -regulatory sequence of interest and introducing it into cells or an organism. (jove.com)
Since the gene of interest and the reporter gene have the same cis -regulatory sequence, they are expressed in the same cells and at the same time. (jove.com)
The cells expressing the Luc gene produce luciferase enzymes that catalyze a reaction with luciferin to produce light. (jove.com)
Reporter gene expression was not affected upon exposure of transiently transfected cells to hypoxia (1% oxygen). (uzh.ch)
Hep G2 cells, transiently transfected with a luciferase reporter containing 1.67 kb of the human tTG promoter, showed an increase in reporter activity after addition of TNF-alpha. (nih.gov)
Furthermore, Hep G2 cells are incapable of upmodulating tTG promoter reporter activity in the presence of TNF-alpha when those cells overexpress a transdominant, negative mutant NF-kappa B subunit. (nih.gov)
In ER+ cancer cells, PIP5K1α acted on pSer-473 AKT, and was in complexes with VEGFR2, serving as co-factor of ER-alpha to regulate activities of target genes including cyclin D1 and CDK1. (lu.se)

However, luciferases have been studied in luminous fungi, like the Jack-O-Lantern mushroom, as well as examples in other kingdoms including bioluminescent bacteria, and dinoflagellates. (wikipedia.org)
Luciferase is the enzyme responsible for the bioluminescence found in a diverse number of organisms, ranging from bacteria to aquatic animals to insects. (addgene.org)

The name was first used by Raphaël Dubois who invented the words luciferin and luciferase, for the substrate and enzyme, respectively. (wikipedia.org)
However, unlike fluorescent proteins, luciferases do not require an external light source, but do require addition of luciferin, the consumable substrate. (wikipedia.org)
The N-terminal domain is conserved between dinoflagellate luciferase and luciferin binding proteins (LBPs). (wikipedia.org)

Positive control for QUANTI-Luc™ , a luciferase reporter assay reagent. (invivogen.com)

It is due to mutations in the glucocerebrosidase gene (GBA) that catalyses the hydrolysis of glucosylceramide (GlcCer). (fundacionareces.es)
To do this, first both the GBA gene mutations and the variations in the promoter of GlcCer synthase are analysed in patients with GD. (fundacionareces.es)

Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein. (wikipedia.org)
Luciferases are widely used in biotechnology, for bioluminescence imaging microscopy and as reporter genes, for many of the same applications as fluorescent proteins. (wikipedia.org)
H. Kwon, T. Enomoto, M. Shimogawara, K. Yasuda, Y. Nakajima and Y. Ohmiya, Bioluminescence imaging of dual gene expression at the single-cell level. (atto.co.jp)
M. Yasunaga, Y. Nakajima and Y. Ohmiya, Dual-color bioluminescence imaging assay using green- and red-emitting beetle luciferases at subcellular resolution. (atto.co.jp)

In addition to its critical role in viral replication, PL pro removes post-translational modifications like ubiquitin and interferon-stimulated gene product 15 (ISG15) from host proteins through its deubiquitinase domain, leading to host immunosuppression and increased ability of the virus to evade the host antiviral immune response. (biorxiv.org)

MicroRNA (miRNA) belongs to the family of single-stranded noncoding RNA with approximately 22 nucleotides that negatively regulates the target genes at the posttranscriptional level, which is involved in a wide range of biological processes [ 11 ]. (hindawi.com)

The effects of PIP5K1α and its inhibitor on growth and invasion of BC were confirmed by using in vitro assays including proliferation, migration, apoptosis and luciferase reporter assays and in vivo xenograft mouse models. (lu.se)

We investigated the expression of genes of interest using quantitative reverse transcription PCR. (molvis.org)
We also demonstrated that the −657 to +411 DCT promoter fragment efficiently directs RB cell-specific transcription of the luciferase reporter gene in cell lines. (molvis.org)
Hypoxia inducible component 1 (HIF-1) is a heterodimeric transcription element that regulates transcription of genes this sort of as vascular endothelial expansion element (VEGF) and primary fibroblast development factor (bFGF) [fourteen,15,sixteen]. (signsin1dayinc.com)
Broccoli extracts, purified Sf, ascorbic acid and PA were tested for ability to induce thioredoxin reductase and quinone reductase transcription in luciferase reporter gene constructs. (usda.gov)
Son and K. Kim, Synchronous activation of gonadotropin-releasing hormone gene transcription and secretion by pulsatile kisspeptin stimulation. (atto.co.jp)

The substrate is then available for oxidation by the luciferase, where it is degraded to coelenteramide with a resultant release of energy. (wikipedia.org)
Luciferase catalyzes the oxidation of a substrate, leading to the emission of photons. (addgene.org)

MicroRNAs (miRNAs) are small noncoding endogenous RNAs containing 19 to 25 nucleotides that promote posttranscriptional control in regulating the expression of target gene by binding to the 3′-UTR sequences of its mRNA [ 5 ]. (hindawi.com)
Whereas the in vitro translation efficiency of these two mRNA isoforms was about equal, the mHIF-1alphaI.2 5'-untranslated region (5'-UTR) conferred significantly higher in vivo luciferase reporter gene activity than the mHIF-1alphaI.1 5'-UTR. (uzh.ch)
Targeted disruption of the gene encoding D4Rs reduces the amplitude of the contrast sensitivity rhythm by reducing daytime sensitivity and abolishes the rhythmic expression of Npas2 and Adcy1 mRNA in the ganglion cell layer (GCL) of the retina. (jneurosci.org)

In addition, Addgene has many luciferase expression constructs available that are driven by a strong constitutive promoter and can be used to monitor transfection or viral infection efficiency, to study changes in cell physiology, or as a reference level of expression. (addgene.org)

This recombinant gene is introduced into different cell types, and both the gene of interest and the reporter gene are allowed to express. (jove.com)
This recombinant DNA was introduced into the worm using a microinjection, and the gene was expressed, similarly to β-tubulin. (jove.com)

It is recommended to use medium-strength promoters (such as TK) rather than strong promoters (such as SV40, CMV), so as not to affect the expression of the main reporter gene, or to conduct experiments to find a suitable vector. (yeasenbiotech.com)
Luciferase measurement of a few unbiased transfection experiments was carried out in triplicate employing 15 ml of the supernatant according to the manufacturer's protocol. (signsin1dayinc.com)

The development of commercially available transfection reagents for gene transfer applications has revolutionized the field of molecular biology and scientific research. (mdpi.com)

Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. (cancerindex.org)
Alternative splicing results in multiple transcript variants of this gene. (cancerindex.org)

Viviani and Y. Ohmiya, Enhanced red-emitting railroad worm luciferase for bioassays and bioimaging. (atto.co.jp)

So, by monitoring the expression of the reporter gene, a scientist can track where the gene of interest is being expressed. (jove.com)

5′-GACGTG-3′) on the promoter region in various target genes ( 1 , 2 ). (spandidos-publications.com)

Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)

The regulatory elements required for this cell-specific gene expression are likely located within its proximal promoter. (molvis.org)

A variety of organisms regulate their light production using different luciferases in a variety of light-emitting reactions. (wikipedia.org)
MicroRNAs (miRNAs) are well-recognized for their abilities to regulate gene expression post-transcriptionally in plants and animals. (frontiersin.org)

The sensitivity and high signal intensity of this luciferase molecule proves advantageous in many reporter studies. (wikipedia.org)
Some of the benefits of using a secreted reporter molecule like MetLuc is its no-lysis protocol that allows one to be able to conduct live cell assays and multiple assays on the same cell. (wikipedia.org)

We identified 150 genes responsive to heat shock including seven HSP genes, six of which belonging to the group of 17 HSP genes enriched in response to heat shock, according to weighted gene co-expression network analysis (WGCNA). (biomedcentral.com)
Many heat responsive genes were reported in these studies. (biomedcentral.com)

In the latter variant, a study is being conducted using functional luciferase reporter genes. (fundacionareces.es)

As expected clones varied in their specific luciferase activity along with the dilutions. (biotech-angels.com)
During pilocarpine-induced SE, in vivo disruption of STEP activity allowed activation of the MAPK pathway, leading to immediate-early gene expression and significant rescue from cell death. (jneurosci.org)
In conclusion, the XBP1-NLuc reporter cell line provided a reliable reporter of IRE1 endonuclease activity, whose expression is increased during the ER stress. (helsinki.fi)
Primary hepatocytes isolated from a transgenic mouse line containing the mouse tTG promoter driving the beta-galactosidase reporter, show similar time-dependent increases in promoter activity when treated with TNF-alpha. (nih.gov)

Mature miRNAs are generated by the RNase III enzymes Drosha and Dicer, then incorporate into the RNA-induced silencing intricate (RISC), and eventually bind to the 39-untranslated location (39-UTR) of their target gene mRNAs, inhibiting their expression[1,2]. (signsin1dayinc.com)
That's big enough to transport more than 38,000 base pairs of DNA, which is enough to supply multiple gene-editing units to a target cell, Bao says. (futurity.org)
Application of the magnetic field allows BV transduction, the payload-delivery process that introduces gene-editing cargo into the target cell. (futurity.org)
Subsequent verification using the reporter assay revealed that CORO1C is an indirect downstream target of YB-1. (biomedcentral.com)

MicroRNAs (MiRNAs) are brief non-coding RNAs (182 nt), which inhibit gene expression. (signsin1dayinc.com)
RNA interference is a conserved pathway common to plants and mammals, where double-stranded RNAs (dsRNAs) suppress expression of genes with complementary sequences [ 1-2 ]. (idtdna.com)

A comprehensive understanding of gene expression in human RB is essential for the development of safe and effective new therapies. (molvis.org)

To investigate the correlation between the polymorphism of estrogen receptor β gene (ESR2) rs3020450 and cancer susceptibility, and explore the epidemiological significance and the effect of ESR2 expression levels on the prognosis of ovarian cancer. (cancerindex.org)

The effect of the promoter variant was further studied by luciferase gene reporter assay in MCF-7, HEK293, HeLa and LNCaP cell lines. (biomedcentral.com)

A：at room temperature (20-22°C). Reaction components such as cell lysate, substrate working solution, etc. need to be adjusted to room temperature, because the reaction rates of the two luciferases are affected by temperature. (yeasenbiotech.com)
There is a large pocket in the β-barrel of the dinoflagellate luciferase at pH 8 to accommodate the tetrapyrrole substrate but there is no opening to allow the substrate to enter. (wikipedia.org)

As expected, ER stress treatment increased the reporter expression, while IRE1 inhibitors decreased the expression of the reporter. (helsinki.fi)

The ATP Cell Viability Luciferase Assay offers a highly sensitive homogenous assay for quantifying ATP in cell cultures. (sigmaaldrich.com)
Store ATP Cell Viability Luciferase Assay at -80°C. Avoid repeated freeze-thaw cycles. (sigmaaldrich.com)
A new technology that relies on a moth-infecting virus and nanomagnets could edit defective genes that give rise to diseases like sickle cell, muscular dystrophy, and cystic fibrosis. (futurity.org)
This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. (cancerindex.org)
Luciferase reporter gene assay of the promoter construct bearing this variant did not suggest a variation of expression in the MCF-7 breast cancer cell line, but indicated a reduction of luciferase expression in both the HEK293 and LNCaP cell lines. (biomedcentral.com)
INDIGO Bioscience's nuclear receptor reporter assays are all-inclusive cell-based assay systems. (thomassci.com)

The catalyzed reaction is: coelenterazine + O2 → coelenteramide + CO2 + photon of light Newer luciferases have recently been identified that, unlike other luciferases, are naturally secreted molecules. (wikipedia.org)

Anatomy19

Organisms22

Diseases1

Chemicals and Drugs80

Analytical, Diagnostic and Therapeutic Techniques and Equipment41

Phenomena and Processes76

Disciplines and Occupations1

Technology, Industry, Agriculture2

Information Science3

Dual luciferase reporter gene4

Encodes8

Firefly16

Protein18

Gaussia3

Multicolor luciferase1

Differentially4

Plasmids5

Luminescence4

Regulation7

Renilla3

Plasmid2

Transcriptional2

Detection3

Cells22

Bacteria2

Luciferin3

Reagent1

Mutations2

Bioluminescence4

Proteins1

MiRNA1

Vivo xenograft1

Transcription5

Oxidation2

MRNA3

Constructs1

Recombinant2

Experiments2

Molecular biology1

Transcript2

Ohmiya1

Monitoring the expression of the reporter1

Promoter Region1

Expression Omnibus1

Regulatory1

Regulate2

Molecule2

Responsive2

Functional1

Activity4

Target4

RNAs2

Understanding of gene expression1

Estrogen1

HeLa1

Substrate2

Inhibitors1

Cell6

Reaction1