Long ncrnas ncrnas rna gene. Medical search. Frequent questions

In the Yildirim lab, we study how epigenetic mechanisms, particularly those that are mediated by long noncoding RNAs (ncRNAs), complement gene expression, impact genome stability and define cell fate decisions. (duke.edu)
Noncoding RNAs (ncRNAs) are noncoding transcripts that take part in epigenetic mechanisms by providing RNA-directed silencing, aiding recruitment of chromatin modifying complexes and in some instances, presenting enhancer-like functions to boost transcription. (duke.edu)
In this review, we summarized the interactions of noncoding RNAs and viruses in the occurrence and development of AR, along with the treatments focusing on the noncoding RNAs in the past five years. (hindawi.com)
Long noncoding RNA (lncRNAs) are vital noncoding RNAs (ncRNAs) that regulate fat accumulation, including the processes of proliferation, differentiation, and hypertrophy of adipocytes [ 6 , 7 ]. (animbiosci.org)
Here we review some of these new discoveries and technologies, including long noncoding RNAs, microRNAs and RNA aptamers, and how they are being applied to prostate cancer research at the Brady Urological Institute. (blogspot.com)
One class of genes which do not code for protein products are called noncoding RNAs (ncRNAs) . (blogspot.com)
This gene class includes long noncoding RNAs (lncRNAs) and microRNAs (miRNAs), both of which have been implicated in cancer [ 4, 5 ]. (blogspot.com)
Long noncoding RNAs are over 200 nucleotides (or building blocks of DNA and RNA) in length and may perform a variety of functions which include the regulation of DNA organizational density in chromosomes and the regulation of gene expression. (blogspot.com)
Dr. Guil has been investigating the functions of RNA in gene expression regulation and specifically the role that noncoding RNAs (ncRNAs) play in disease for more than 15 years. (carrerasresearch.org)
In the newly identified territories are found ncRNAs (noncoding RNAs, not destined for protein)-thousands of them-that are doing important jobs: guiding regulators into the right territories where 'essential nuclear functions' need to take place. (practicalbible.com)
Noncoding RNAs (ncRNAs), such as microRNAs and long noncoding RNAs (lncRNAs), participate in cellular transformation. (oncotarget.com)
Approximately just 2% of the genome is transcribed into protein-coding RNAs [ 1 ], so the majority of transcripts are noncoding RNAs (ncRNAs), that can be categorized according to their structural properties and length [ 2 ]. (oncotarget.com)
In recent years, noncoding RNAs (ncRNAs), especially microRNAs, long noncoding RNAs and circular RNAs, have been found to be associated with abnormalities of the ASMCs. (ersjournals.com)
This review highlights findings concerning noncoding RNAs (ncRNAs) in asthmatic ASMCs so far and proposes a schematic diagram that helps us to better understand how ncRNAs are involved in pathophysiological behavioural changes in asthmatic ASMCs. (ersjournals.com)
Many noncoding RNAs (ncRNAs) have been identified in asthmatic ASMCs in recent years [ 5 ]. (ersjournals.com)
MicroRNAs (miRNAs) are the most altered subset of ncRNAs, followed by long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs). (ersjournals.com)
Long noncoding RNAs (lncRNAs) play crucial roles in tumorigenesis, and lncRNA taurine-upregulated gene 1 ( TUG1 ) has been proven to be associated with several human cancers. (biomedcentral.com)
Therefore, diverse cellular compartments orchestrated by multiple environment-driving factors make the mechanisms underlying asthma extraordinary complex and unclear, particularly with respect to long noncoding RNAs (lncRNAs). (researchsquare.com)

In this review, we focus on the impact of ncRNA post-transcriptional regulatory mechanisms, especially those of microRNAs and lncRNAs, in RA signalling pathways during differentiation and disease. (mdpi.com)
MicroRNAs (miRNAs or miRs) are involved in the post-transcriptional regulation of gene expression via complete or partial base pairing with the 3′untranslated region of target genes ( 3 ). (spandidos-publications.com)
The small microRNAs have opened the field of ncRNAs capturing the attention of basic and clinical scientists for their potential to become new therapeutic targets and clinical biomarkers for aortic aneurysm. (derby.ac.uk)
This class of RNA encompasses microRNAs (miRNAs, well-studied post-transcriptional regulators of gene expression), long non-coding RNAs (lncRNAs) and other ncRNAs. (gencat.cat)
MicroRNAs, on the other hand, are smaller RNAs which primarily function as RNA interference agents, to block the synthesis of proteins encoded by specific coding genes. (blogspot.com)
MicroRNAs are the physically smaller class of ncRNAs. (blogspot.com)
Currently, there are over 1,000 known human microRNAs, each of which is predicted to regulate hundreds to thousands of protein coding genes [ 12] . (blogspot.com)
MicroRNAs (miRNAs) are small non-coding RNAs that function as guide molecules in RNA silencing. (gene-quantification.com)
MicroRNAs are small, non-coding RNA molecules that can complementarily bind to the mRNA 3'-UTR region to regulate the gene expression by transcriptional repression or induction of mRNA degradation. (gene-quantification.com)
many studies have shown that small RNA molecules called microRNAs circulate in the blood in cell-free mode. (gene-quantification.com)
MicroRNAs display exquisite stability in serum or plasma because they are packaged in membrane-encapsulated vesicles or protected by RNA-binding proteins. (gene-quantification.com)
MicroRNAs also survive in unfavorable physiological conditions, such as repeated freeze-thawing, and long-term storage at room temperature. (gene-quantification.com)
SncRNAs are less than 200 nt in length, consisting of microRNAs (miRNAs), Piwi-interacting RNAs (piRNAs), endogenous small interfering RNAs (endo-siRNAs or esiRNAs) and transcription initiation RNAs (tiRNAs) [ 6 ]. (ijbs.com)

NEAT1) sequester various regulatory proteins regulating gene expression including RNA modification (e.g. (bvsalud.org)
Non-coding RNA are a class of RNA that lack the potential to encode proteins. (bvsalud.org)
During the Human Genome Project, most efforts to estimate and annotate genes focused on protein-coding genes, i.e., regions of the genome that are transcribed into RNA and then translated into proteins. (biomedcentral.com)
Some of them are the protein-coding genes for RNA polymerase subunits, ribosomal proteins, enzymes of lipid metabolism, and many other enzymes. (blogspot.com)
Traditionally RNA has been viewed as a simple messenger that translates genetic information from our DNA ( deoxyribonucleic acid) into proteins that are used by the cells in our body. (blogspot.com)
In a cell, DNA is "translated" by RNA into proteins that can be used by the cell throughout the body. (blogspot.com)
It is estimated that coding genes (the genes which code for the ~20,000 human proteins) only account for ~1.5% of the three BILLION nucleotides of sequence information in the human genome. (blogspot.com)
There, she studied the regulatory action of RNA binding proteins upon stress stimuli, and, particularly, their role as controllers of specific microRNA biogenesis. (carrerasresearch.org)
Gene Ontology and pathway enrichment analyses and assessment of RNA binding proteins were performed by R software, circlncRNAnet and Encyclopedia of RNA Interactomes (ENCORI). (xiahepublishing.com)
These vesicles are carriers of active or non-autonomous function biomolecules, such as proteins, lipids, DNA, mRNA and non-coding regulatory RNA. (spandidos-publications.com)
The fate of cells is determined by epigenetics, as it plays a role in allowing the heart cell to turn "on" genes to make proteins important for its job and turn "off" genes important for an immune cell's job. (troscriptions.com)
More than half of all human genes are alternatively spliced, so it is expected that alternative pre- mRNA splicing will be responsible for the identification of more than 90 percent of all human proteins. (genomicglossaries.com)
Epigenetic components (for example, Polycomb PRC1/2 and Trithorax group proteins) maintain the 'off' states of certain genes and the 'on' states of others, in a cell-type- and time-specific manner (the bottom panels show three genes, depicted schematically as chromatinized templates, in which transcription is triggered by specific transcription factors and silent or active states are maintained by PRC1/2 or Trithorax proteins, respectively). (nature.com)

Circular RNAs (circRNAs) are closed, long, non-coding RNAs that are formed via direct reverse splicing of precursor mRNA that function by regulating RNA transcription and protein production, as well as sponging miRNAs ( 4 ). (spandidos-publications.com)
They include coding mRNAs and non-coding (nc) RNAs among them antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), micro-RNAs (miRNAs), small activating RNAs (saRNAs), RNA aptamers and RNA guides. (frontiersin.org)
With the increasing technical depth of RNA profiling technologies, novel insights have unravelled the spatial diversity exhibited by miRNAs inside a cell. (eurekaselect.com)
This review provides a bird's eye view of miRNAs expressed in different subcellular locations, thus affecting the gene regulatory pathways therein. (eurekaselect.com)
Increasing evidence suggests a new mechanism by which miRNAs may regulate target gene expression by binding in promoter and amino acid coding regions. (gene-quantification.com)
To address this issue, we present miRWalk, a comprehensive database on miRNAs, which hosts predicted as well as validated miRNA binding sites, information on all known genes of human, mouse and rat. (gene-quantification.com)
These results are consistent with a previous model that miRNAs might have been derived from inverted-duplication of targeted protein-coding genes [ 7 ]. (biomedcentral.com)
We summarise the pathophysiological regulatory processes of ncRNAs, especially miRNAs, lncRNAs and circRNAs, in hyperplasia, hypertrophy, ECM production and cytokine production in asthmatic ASMCs. (ersjournals.com)
MiRNAs were identified as single-stranded ncRNAs with a length of approximately 22 nucleotides in 1993 [ 14 ]. (ersjournals.com)
Initially, two observations prompted the present study: i) previous results revealed down-regulation of p68 RNA helicase resulting from overexpression of the IDX hairpin loop in HeLa cells, and ii) the secondary structure of the IDX hairpin loop resembles pri-miRNAs, implying that an miRNA could be processed from the hairpin loop-containing pri-miRNA and regulate 68 RNA helicase. (lidsen.com)

The Kim lab is particularly interested in the role of micro-RNAs (miRNA) and long intergenic non-coding RNAs (lincRNAs). (umaine.edu)
According to its size, regulatory ncRNAs can be further classified as small ncRNA ( ∼ 18-31nt, such as miRNA, siRNA, and piRNA), medium ncRNA ( ∼ 31-200nt) and long ncRNA (from 200nt up to several hundred kb, such as lincRNA and microRNA) [ 4 ]. (biomedcentral.com)
All mRNAs, mitochondrial genes and 10 kb upstream flanking regions of all known genes of human, mouse and rat were analyzed by using a newly developed algorithm named 'miRWalk' as well as with eight already established programs for putative miRNA binding sites. (gene-quantification.com)
Such a resource enables researchers to validate new targets of miRNA not only on 3'-UTR, but also on the other regions of all known genes. (gene-quantification.com)
The birth and evolution of miRNA genes is an issue of considerable interest as it could potentially help investigators understand how miRNA-based regulation networks adapt to various environments. (biomedcentral.com)
MiRNA usually binds directly to the 3′-untranslated region (3′-UTR) of target gene mRNA through the RNA-induced silencing complex (RISC) [ 15 , 16 ]. (ersjournals.com)
More importantly, circRNAs serve as microRNA (miRNA) sponges and crucial regulators of gene expression. (ijbs.com)
Furthermore, RNAi assays containing the hairpin loop as an miRNA precursor were conducted, using the pTer vector, to explore the effects on 68 RNA helicase expression levels. (lidsen.com)

LncRNAs are a class of ncRNAs of 200 nucleotides in length. (bvsalud.org)
This sRNA sequence is 138 nucleotides long and is found in Caenorhabditis elegans. (rnacentral.org)
LncRNAs, little or no coding potential, are defined as capped transcripts longer than 200 nucleotides. (biomedcentral.com)

These results suggest that the noncoding RNA regulatory network may play a potential role in the development of allergic rhinitis (Figure 1 ). (hindawi.com)
HOX transcript antisense RNA (HOTAIR), a trans-acting lncRNA with regulatory function on transcription, can repress gene expression by recruiting chromatin modifiers. (bvsalud.org)
PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element. (nature.com)
Few regulatory studies are available to assist in the regulatory categorization of RNA-based medicinal products. (frontiersin.org)
From 2014, Sonia Guil leads the "Regulatory RNA and chromatin" research team, first at the Bellvitge Biomedical Research Institute (IDIBELL) in L'Hospitalet de Llobregat, Barcelona, and from 2019 at the IJC. (carrerasresearch.org)
Recent studies have suggested that eukaryotic genomes encode a large number of functional transcripts of non-coding RNAs (ncRNAs), including housekeeping and regulatory RNAs [ 1 - 3 ]. (biomedcentral.com)
We also discuss the huge potential of the ncRNA regulatory network as the basis for diagnosis and treatment of ASMC dysfunction. (ersjournals.com)
Notably, circRNAs display cell-type, tissue-type and developmental-stage specific expression patterns in eukaryotic transcriptome, which reveals their significant regulatory functions in gene expression. (ijbs.com)
Non-coding RNAs (ncRNAs) have emerged as one of the most abundant regulatory molecules. (lidsen.com)

Circular RNAs (circRNAs) are molecular sponges that are involved in regulation of multiple types of cancer. (spandidos-publications.com)
CircRNAs, generated by a post-splicing mechanism, are a newly discovered type of non-coding RNA with multi-functional covalent loop structures. (bvsalud.org)
Herein, we wondered whether circular RNAs (circRNAs), a type of RNA transcripts lacking 5′-3′ ends and forming closed loops that are gaining relevance in cancer biology, are also a target of epigenetic inactivation in tumors. (oncotarget.com)
Circular RNAs (circRNAs), a novel type of non-coding RNAs (ncRNAs), are ubiquitously expressed in eukaryotic cells during post-transcriptional processes. (ijbs.com)
Unlike linear RNAs, circRNAs form covalent-closed continuous loops without 5' to 3' polarities and poly (A) tails. (ijbs.com)
Circular RNAs (circRNAs), a novel member of the lncRNA kingdom, are generated by non-sequential backsplicing of exons, introns or a combination of both [ 7 - 9 ]. (ijbs.com)
CircRNAs are highly stable forms of ncRNAs due to their nuclease resistance properties [ 11 ]. (ijbs.com)
CircRNAs were first identified in RNA viruses in the 1970s [ 12 - 14 ]. (ijbs.com)
However, circRNAs were initially regarded as viral genomes or byproducts of pre-mRNA alternative splicing, and thus they did not gain much attention for a long time [ 15 ]. (ijbs.com)

Cumulative evidence shows that only ∼ 2 percent of protein-coding genes are in the human genome and the remaining ∼ 98 percent of the human genome are classified as non-coding RNAs (ncRNAs) [ 1 ]. (biomedcentral.com)
This generates a vast repertoire of transcripts that includes protein-coding messenger RNAs, long non-coding RNAs (lncRNAs) and repetitive sequences, such as SINEs (short interspersed nuclear elements). (nature.com)
Antisense Uchl1 function is under the control of stress signalling pathways, as mTORC1 inhibition by rapamycin causes an increase in UCHL1 protein that is associated to the shuttling of antisense Uchl1 RNA from the nucleus to the cytoplasm. (nature.com)
Antisense Uchl1 RNA is then required for the association of the overlapping sense protein-coding mRNA to active polysomes for translation. (nature.com)
These include 224 novel protein-coding genes and 116,156 novel transcripts. (biomedcentral.com)
Sandwalk: How many human protein-coding genes are essential for cell survival? (blogspot.com)
The human genome contains about 20,000 protein-coding genes and about 5,000 genes that specify functional RNAs. (blogspot.com)
Each group identified between 1500 and 2000 protein-coding genes that are essential in their chosen cell lines. (blogspot.com)
One of the annoying things about all three papers is that they use the words "gene" and "protein-coding gene" as synonyms. (blogspot.com)
The only genes they screened were protein-coding genes but the authors act as though that covers ALL genes. (blogspot.com)
2015). What they meant is that they represent 9.2% of protein-coding genes. (blogspot.com)
The HSP70 gene family encodes the major heat shock protein of molecular weight 70,000. (blogspot.com)
1000 Mb with up to 18,000 protein-coding genes [ 12 ]. (biomedcentral.com)
Transcriptome-wide identification of RNA-binding protein and microRNA target sites by PAR-CLIP. (eurekaselect.com)
Over the last several decades, however, new roles for RNA have been discovered that go well beyond the simple beauty of protein coding. (blogspot.com)
For instance, there are segments of RNA that do not carry a code for a protein, but instead act by influencing how the information in DNA and RNA is used. (blogspot.com)
In fact, much of our understanding about cancer biology today stems from the discovery of mutations (or alterations) in protein-coding genes, and how the resulting protein products can drive cancer development, progression, and metastasis [ 2] . (blogspot.com)
Recent research has uncovered a large number of genomic loci encoding long or small RNAs with low protein-coding potential. (biomedcentral.com)
Exosomes are one of the most researched classes of extracellular vesicles because they are carriers of targeted protein and DNA/RNA loads. (spandidos-publications.com)
Because alternative RNA splicing allows different types of mRNA molecules to be created from a single gene, it generates the diversity of protein function and structure that is essential to complex organisms. (genomicglossaries.com)
Highly specific and effective gene silencing of any disease can be achieved by an accurate knowledge of the target mRNA sequence and rational design of its complementary antisense agents for the downregulation of its protein message. (genomicglossaries.com)
In particular, we have identified that promoter CpG island hypermethylation of the genes TUSC3 (tumor suppressor candidate 3), POMT1 (protein O-mannosyltransferase 1), ATRNL1 (attractin-like 1) and SAMD4A (sterile alpha motif domain containing 4A) is linked to the transcriptional downregulation of both linear mRNA and the hosted circRNA. (oncotarget.com)
Protein-coding and non-coding genes, splice variants, cDNA and protein sequences, non-coding RNAs. (ensembl.org)
The results demonstrated that TUG1 RNA was bound to enhancer of zeste protein 2/embryonic ectoderm development (EZH2/EED), which is essential for PRC2. (biomedcentral.com)
Studies of human genome demonstrate that protein-coding genes only occupy less than 2% of the entire genome [ 1 ]. (ijbs.com)
Author Manuscript and RNA repair protein AlkB-derived enzyme mixture was first used to remove methylations at the Watson-Crick face. (cdc.gov)

Gene expression, Kaplan-Meier survival, microRNA and transcription factor target analyses were performed with the University of Alabama Cancer (UALCAN) Database, Kaplan-Meier Plotter, LinkedOmics, WebGestalt and gene set enrichment analysis, respectively. (xiahepublishing.com)
nc886 is a 101 nucleotide long non-coding RNA that has been designated as a precursor microRNA or a vault RNA based upon it sequence. (oncotarget.com)
All these activities of nc886 are undoubtedly independent of mature microRNA or vault RNA. (oncotarget.com)

Recent studies have shown ncRNA to be involved in many important biological processes such as embryonic development, transcriptional regulation, apoptosis, and immunity. (umaine.edu)
A large number of experimental studies show that the mutation and regulation of long non-coding RNAs (lncRNAs) are associated with various human diseases. (biomedcentral.com)
Long non-coding RNAs (lncRNAs) play an increasingly important role in some fundamental biological processes such as translational regulation, cell cycle regulation, epigenetic regulation, splicing, differentiation, and immune response [ 5 ]. (biomedcentral.com)
Hastings, M. L., Ingle, H. A., Lazar, M. A. & Munroe, S. H. Post-transcriptional regulation of thyroid hormone receptor expression by cis-acting sequences and a naturally occurring antisense RNA. (nature.com)
RNA-seq performed in humans has determined the molecular regulation mechanisms of fat accumulation between different groups of samples with different genetic backgrounds. (animbiosci.org)
She was enrolled in the Cancer Epigenetics and Biology Program of the Bellvitge Biomedical Research Institute (IDIBELL), under the direction of Dr. Esteller, where she broadened her research interests to include the epigenetic control as a major determinant of ncRNA-mediated regulation in transcriptional and post-transcriptional stages in both normal and pathological conditions. (carrerasresearch.org)
In her group, they combine a battery of biochemical and global genomic approaches, both in vitro and in vivo to dissect mechanisms of gene expression regulation with the participation of ncRNAs, with the ultimate goal of contributing to devise the best RNA-based therapeutic strategies in different pathological settings. (carrerasresearch.org)
These maps reveal higher-order RNA-chromatin structures associated with three major classes of nuclear function: RNA processing, heterochromatin assembly, and gene regulation. (practicalbible.com)
Functional enrichment analysis revealed SNHG4 involvement with regulation of ribosomal RNA synthesis and the RNA processing and surveillance pathway. (xiahepublishing.com)
Molecular and in silico studies of such non-coding RNAs (ncRNAs) have expanded our understanding of gene transcriptional regulation and the generation of organism complexity. (biomedcentral.com)
The most common PTMs which are well-studied and understood in the context of DNA repair, gene expression, and regulation are acetylation and methylation. (troscriptions.com)
The lab investigates mechanisms of RNA mediated epigenetic gene regulation by employing a combination of biochemistry, cell and molecular biological methods, and genome wide approaches. (lncrnablog.com)
Among these candidate genes, CUGBP and Elav-like family member 1 ( CELF1 ) are potential targets of TUG1 in-trans regulation. (biomedcentral.com)
RNA immunoprecipitation was then performed to examine whether TUG1 RNA was bound to PRC2, a TUG1 -involved regulation mechanism reported in previous studies. (biomedcentral.com)
Using TUG1 4C sequencing and bioinformatic analysis, we found CELF1 to be a potential target of TUG1 RNA in in-trans regulation. (biomedcentral.com)
lncRNAs have been reported to possess multiple functions including regulation of gene expression, transcriptional activation and silencing of genes and thus play critical roles in a diversity of cellular process [ 14 , 15 ]. (researchsquare.com)

While the first mRNA vaccines against an infectious disease have reached the market ( 1 , 2 ), many other medicinal products with RNA as an active substance (see Table 1 ), either vaccines against non-infectious diseases or non-vaccine drugs, are under development ( 3 - 18 ) or even approved ( 19 - 25 ). (frontiersin.org)
There are currently several products containing RNA on the market, and many are under development, among them mRNA, antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), small activating RNAs (saRNAs), micro-RNAs (mi-RNAs), RNA aptamers and RNA guides. (frontiersin.org)
High- fidelity mRNA amplification for gene profiling. (genomicglossaries.com)
RNA-like oligonucleotides that are complementary to a portion of a target mRNA molecule. (genomicglossaries.com)
More specifically, antisense oligonucleotides that are useful as reagents for target validation , or as drugs, are engineered molecules that differ from natural RNA but that have a base sequence that is recognized as being complementary to a very specific mRNA sequence. (genomicglossaries.com)
A complementary RNA sequence that binds to a naturally occurring (sense) mRNA molecule, thus blocking its translation. (genomicglossaries.com)
Here, we highlight that an important RNA sequence region, encompassing an exon-intron hairpin loop (also called IDX-rasISS1), of the H-Ras pre-mRNA may encode an ncRNA that regulates p68 RNA helicase. (lidsen.com)

In the long run, a detailed understanding of the genetic, epigenetic, and cellular mechanisms that are mediated by long ncRNAs and spatial positioning will allow us to explore their causal roles in disease and cancer. (duke.edu)
This review introduces the different ncRNA types and focus at ncRNA roles in aorta aneurysms. (derby.ac.uk)
Since 2008, she has received funding from both public and private agencies to carry out research involving the study of the emerging roles of both short and long ncRNAs. (carrerasresearch.org)
Increasingly evidences suggest that long intergenic ncRNAs (lincRNAs) are developmentally regulated and play important roles in development and stress responses of plants. (biomedcentral.com)
ncRNAs play important roles in controlling gene expression at both transcriptional and post-transcriptional levels. (ijbs.com)

Our long-term goal is to delineate the molecular bases and order of genetic, epigenetic and cellular processes that link altered expression of long ncRNAs and pathways driven by these molecules to cancer. (duke.edu)
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (genomicglossaries.com)
Conclusions Our study successfully discovered many key noncoding RNA molecules related to pathogenesis of asthma and relevant treatment, which may provide novel biomarkers and therapeutic targets for asthmatic diagnose and therapy. (researchsquare.com)

HGNC also coordinates with the related Mouse and Rat Genomic Nomenclature Committees, other database curators, and experts for given specific gene families or sets of genes. (wikipedia.org)
With the development of high-throughput sequencing, genome-wide association studies (GWAS) and RNA sequencing (RNA-seq) have been extensively used in recent years to identify genomic regions and candidate genes associated with a variety of pork traits [ 3 - 5 ]. (animbiosci.org)
However in recent years with the development of epigenesis, genomic imprinting and the horizontal transferences of the genes, Lamarck's ideas have resurfaced. (intechopen.com)
The Cancer Genome Atlas (TCGA) database has accumulated abundant genomic and gene expression profiles for different diseases in the past decade. (xiahepublishing.com)
nc886 has also been suggested to be a tumor suppressor, mainly inferred by its expression pattern as well as its genomic location at human chromosome 5q31, a locus for a tumor suppressor gene(s). (oncotarget.com)
However, it has been proven that more than 62% of genomic DNA serves as a template for transcription, which indicates that there are abundant non-coding RNAs (ncRNAs) in human transcriptome [ 2 , 3 ]. (ijbs.com)

Our laboratory uses primarily XCI as a model to understand how long ncRNAs 1) regulate gene dosage and maintain epigenetic state, 2) impact genome stability, and 3) participate in cell fate decisions. (duke.edu)
In various organisms, components of the nuclear envelope interact with chromatin that is spatially organized within the nucleus (Fig 2) and subsequently regulate gene expression. (duke.edu)
Our aim is to define the molecular bases of the interactions that are established between chromatin and the components of the nuclear envelope, and determine how they 1) regulate epigenetic state of genes and their transcription, 2) participate in spatial organization of the genome, and 3) contribute to cellular functions during mammalian development. (duke.edu)
In plants, the most well studied ncRNAs are 21 to 24 nucleotide small RNAs (smRNAs) that negatively regulate specific targets through complementary sequences. (biomedcentral.com)
Moreover, subsequent experiments showed that TUG1 RNA could bind to PRC2 in the promotor region of CELF1 and negatively regulate CELF1 expressions in H520 cells. (biomedcentral.com)

However, identification of lincRNAs in wheat is still limited comparing with functional gene expression. (biomedcentral.com)

Here we identify a nuclear-enriched lncRNA antisense to mouse ubiquitin carboxy-terminal hydrolase L1 ( Uchl1 ), a gene involved in brain function and neurodegenerative diseases 11 . (nature.com)
Use of the microarray analysis is often restricted by the known gene sequences arrayed on the chip, whereas RNA sequencing is not dependent on pre-existing databases of expressed genes and, therefore, provides an unbiased and more complete view of gene expression profiles [ 17 ], including lncRNA. (biomedcentral.com)
The lncRNA taurine-upregulated gene 1 ( TUG1 ) is a nonprotein-coding gene located on chromosome 22q12.2 that transcribes to a 6.7-kilobase-long, spliced, and polyadenylated RNA. (biomedcentral.com)

Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed on differentially expressed genes (DEGs). (spandidos-publications.com)
5%, L). Whole-transcriptome sequencing was performed to explore the differentially expressed (DE) genes between these two groups. (animbiosci.org)
This RNA was originally discovered at the Brady Urological Institute in the laboratory of William Isaacs, through a molecular biology screen designed to find genes that are differentially expressed in healthy and cancerous prostate tissues [ 7] . (blogspot.com)

We assembled the sequences from deep RNA sequencing experiments by the Genotype-Tissue Expression (GTEx) project, to create a new catalog of human genes and transcripts, called CHESS. (biomedcentral.com)
As a result, the transcript generated from this vector does not correspond precisely to the selected non-coding RNA gene, but contains some additional sequences both upstream and downstream. (vectorbuilder.kr)
The pluripotency of the initial cell and the establishment of cell types depend to a large extent on the coordinated deployment of hundreds of transcription factors that bind to specific DNA sequences to activate or repress the transcription of cell lineage genes 1 . (nature.com)
To validate our hypothesis , we directly compared p68 RNA sequences and the hairpin loop in silico . (lidsen.com)
Thus it can be inferred from our findings that the alternative splicing exon IDX from H-Ras, coupled with the immediately downstream intron sequences, may contain an ncRNA. (lidsen.com)

Some of these genes are the genes for ribosomal RNA, tRNAs, the RNAs involved in splicing, and many other types of RNA. (blogspot.com)

87(5): 1663-1667] Specifically, the investigators started by priming whole cerebellar RNA with a synthetic oligonucleotide containing a T7 RNA polymerase promoter sequence. (genomicglossaries.com)
After second- strand cDNA synthesis, T7 RNA polymerase was used to generate aRNA. (genomicglossaries.com)
The AAV non-coding RNA expression vector uses an RNA polymerase II promoter to drive the expression of the user-selected non-coding RNA gene. (vectorbuilder.kr)
For RNA polymerase II-mediated transcription, the start site is typically in the 3' region of the promoter while the termination site is within the polyA signal sequence. (vectorbuilder.kr)

Long non-coding RNAs (lncRNAs) are emerging as crucial regulators of gene expression and physiological processes. (bvsalud.org)
Non-coding RNA (ncRNAs) are emerging as new fundamental regulators of gene expression. (derby.ac.uk)
These data demonstrate that hundreds of ncRNAs form high-concentration territories throughout the nucleus, that specific RNAs are required to recruit various regulators into these territories, and that these RNAs can shape long-range DNA contacts, heterochromatin assembly, and gene expression. (practicalbible.com)
Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. (genomicglossaries.com)

The datasets included transcriptome-wide expression and matched clinical follow-up data to detect and characterize gene fusions in PCa. (biomedcentral.com)
Results In the present study, we performed a systematic transcriptome analysis based on the deep RNA sequencing of ten asthmatic children before and after desensitization treatment. (researchsquare.com)

Candidates with research interests in chromatin biology, epigenetics, and non-coding RNAs are encouraged to apply. (lncrnablog.com)

Mammalian X chromosome inactivation (XCI) provides a powerful model for studying the interplay between epigenetic mechanisms driven by ncRNAs and development. (duke.edu)
In order to better understand and characterize ncRNAs it is critical to study their mechanisms in vivo. (umaine.edu)

Long non-coding RNA LINC00926 regulates WNT10B signaling pathway thereby altering inflammatory gene expression in PTSD. (nih.gov)
We are interested in defining the molecular bases of these interactions and delineating their significance in driving gene expression and genome functions. (duke.edu)
Data from genome wide expression profiling studies of various human cancers have provided evidence for altered expression of long ncRNAs. (duke.edu)
This female specific dosage compensation mechanism depends on expression of Xist long ncRNA, which coats and transcriptionally silences future inactive X (Xi) balancing X-linked gene expression between XX females and XY males (Fig 1). (duke.edu)
In addition to their canonical functions in maintaining nuclear architecture and nucleocytoplasmic trafficking, components of the nuclear envelope (i.e. nuclear pore complex (NPC), nuclear lamina) are also involved in intracellular signaling, DNA repair, and gene expression. (duke.edu)
FUT6 and CGNL1 were downregulated in HNSCC compared with adjacent healthy tissue and the expression levels of these genes were associated with tumor stage. (spandidos-publications.com)
More interestingly, a large number of noncoding fragments were found in peripheral blood cells of patients with asthma, including natural antisense chains, pseudogenes, and differential expression of ncRNA between genes [ 14 ]. (hindawi.com)
In addition, Th2 cells are important mediators of type I allergy, and it has been reported that ncRNA promotes Th2 cell migration to target organs by regulating chemokine gene expression [ 17 ]. (hindawi.com)
Forced expression of HOTAIR in epithelial cancer cells induces genome-wide Polycomb repression complex 2 (PRC2) to retarget to a more similar pattern of embryonic fibroblasts, leading to gene expression changes, and increase cancer invasion and metastasis. (biomedcentral.com)
These data reveal another layer of gene expression control at the post-transcriptional level. (nature.com)
A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial-mesenchymal transition. (nature.com)
Many software packages that analyze RNA sequencing (RNA-seq) experiments, which measure gene expression, rely on a database of known genes and cannot measure genes or splice variants unless they are included in the database. (biomedcentral.com)
Furthermore, the appropriate analysis and reliable interpretation of the large datasets obtained by GWAS and RNA-seq is critical for gene expression studies. (animbiosci.org)
These are responsible for abnormal gene expression, resulting in increased cancer cell proliferation and escape from immune surveillance, which can speed up the process of development into HCC. (xiahepublishing.com)
DNA methylation in the promoter regions acts as a repressor of gene expression. (troscriptions.com)
The histones are subjected to various post-translational modifications (PTMs), which play a vital role in gene expression. (troscriptions.com)
A biological process that is crucial for gene expression in most animal life, including humans. (genomicglossaries.com)
presents an opportunity to manipulate gene expression within the cells to treat various diseases, and acts as a powerful tool for studying gene function utilizing antisense agents to manage the diseases by regulating the expression of the specific factor that actually causes the particular disease. (genomicglossaries.com)
The AAV non-coding RNA expression vector is a highly efficient vehicle for in vitro and in vivo delivery of non-coding RNAs of interest. (vectorbuilder.kr)
The AAV non-coding RNA expression vector is first constructed as a plasmid in E. coli. (vectorbuilder.kr)
Cellular identity is now accepted to be the result of the expression of specific combinations of genes (Fig. 1 ). (nature.com)
Finally, higher-order 3D chromosome folding is also thought to modulate gene expression and might contribute to inheritance 15 . (nature.com)
On the other hand, environmental factors have been reported to modulate epigenetic modifications and thereby affect gene expression and phenotype [ 11 , 12 ]. (researchsquare.com)
We also unveil one potential function of this ncRNA whose expression is regulated by alternative splicing decisions. (lidsen.com)
High-throughput RNA sequencing (RNA-seq) has revolutionized our understanding of gene expression. (cdc.gov)

However, how long ncRNA-mediated pathways may impact tumorigenesis is still poorly understood. (duke.edu)

This article will discuss the three main epigenetic signatures (DNA methylation, Histone Modification, and noncoding RNA), how they are regulated, and how their disruption cause diseases. (troscriptions.com)
DNA methylation is one of the key epigenetic modifications that play a role in regulating genes. (troscriptions.com)

RNA maps reveal new RNA classes and a possible function for pervasive transcription. (nature.com)

I want to know about specific genes so I can see if the results conform to expectations. (blogspot.com)
Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes. (genomicglossaries.com)

We discuss the potential of therapeutic interventions targeting ncRNAs and we describe the research models allowing for mechanistic studies and clinical translation attempts for controlling aneurysm progression. (derby.ac.uk)
In addition to these natural non-coding RNAs, new techniques have been evolved to artificially generate RNAs as novel cancer diagnostic, prognostic and therapeutic agents. (blogspot.com)

This gene has 2 transcripts (splice variants). (medchemexpress.cn)

RNA which contains an intron sequence that has an enzyme- like catalytic activity. (genomicglossaries.com)

The HGNC has also issued guides to specific locus types such as endogenous retroviral loci, structural variants and non-coding RNAs. (wikipedia.org)

Work done in the last decade has also demonstrated that ncRNAs with growth-inhibitory functions can undergo promoter CpG island hypermethylation-associated silencing in tumorigenesis. (oncotarget.com)

Biologists have long sought to understand how a fertilized egg can form an organism composed of hundreds of specialized cell types, each expressing a defined set of genes. (nature.com)

Author Manuscript class of cellular RNA for which the standard sequencing methods cannot yet be applied efficiently and quantitatively, although attempts have been made (e.g. ref 2). (cdc.gov)

Despite its biological importance, transfer RNA (tRNA) could not be adequately sequenced by Author Manuscript standard methods due to abundant post-transcriptional modifications and stable structure, which interfere with cDNA synthesis. (cdc.gov)
Widely used RNA-seq methods start with adapter ligation and cDNA synthesis of biological RNA samples followed by PCR amplification to generate sequencing libraries1. (cdc.gov)

Caenorhabditis elegans Unclassified non-coding RNA F56A11.13 sequence is a product of F56A11.13 gene. (rnacentral.org)

We lose methylation capacity as we age, leading to more genes turned on errantly. (troscriptions.com)

However, few reports on genome-wide lncRNAs are available in bread wheat using high-throughput RNA sequencing. (biomedcentral.com)

Genome-wide association studies (GWAS) attempt to link genetic variants to nearby genes, relying on standard catalogs of human genes. (biomedcentral.com)
Concretely, genome-wide association studies have detected numerous asthma-associated gene variants, while few of them (less than 10%) can be explained to contribute to the risk of asthma [ 10 ]. (researchsquare.com)

This study was performed to explore the gene profile and identify candidate long noncoding RNA (lncRNAs) and mRNAs associated with IMF deposition among Laiwu pigs with different IMF contents. (animbiosci.org)
Intersection analysis of the target genes of DE lncRNAs and mRNAs revealed seven candidate genes associated with IMF accumulation. (animbiosci.org)
Finally, alternative splicing microarrays containing apoptosis targets were incubated to verify whether pre-mRNAs other than H-Ras could also present a similar hairpin loop structure regulated by p68 RNA helicase. (lidsen.com)

Moreover, as such a unique noncoding RNA at the time, it had been difficult to determine its biological function. (blogspot.com)

Nuclear bodies in which architectural LONG NCRNAS (e.g. (bvsalud.org)
A large percentage of ncRNAs are nuclear-enriched with unknown function 4 . (nature.com)

Chemicals and Drugs20

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Phenomena and Processes10

Disciplines and Occupations1

Information Science3

Noncoding RNAs18

MicroRNAs13

Proteins13

MiRNAs10

MiRNA8

Nucleotides3

Regulatory9

CircRNAs9

Protein26

MicroRNA3

Regulation16

MRNA7

Roles5

Molecules3

Genomic6

Regulate5

LincRNAs1

LncRNA3

Differentially expressed3

Sequences5

Ribosomal1

Polymerase4

Regulators4

Transcriptome2

Chromatin1

Mechanisms2

Expression25

Pathways1

Epigenetic2

Transcription1

Specific genes2

Therapeutic2

Splice variants1

Intron1

Endogenous1

Tumorigenesis1

Organism1

Cellular1

CDNA synthesis2

Sequence1

Methylation1

High-throughput1

Genome-wide as2

MRNAs3

Biological1

Nuclear2