• Long non-coding RNAs (lncRNAs) participate in regulating biological processes, especially host-pathogen interactions. (biomedcentral.com)
  • A RNA sequencing method (RNA-seq) was used to confirm the differential expression profiles of lncRNAs in Plasmodium yeolii 17XL ( P.y 17XL)-infected BALB/c mice. (biomedcentral.com)
  • Subsequently, the effect of specific lncRNAs on the modulation of immune-related signaling pathways in malaria was determined by fluorescence-activated cell sorting, western blot and enzyme-linked immunosorbent assay. (biomedcentral.com)
  • In addition, about 250 noncoding RNA sequences-including well-characterized long noncoding RNAs (lncRNAs), such as the XIST transcripts that have a key role in X-chromosome inactivation-are decorated by m 6 A. In almost all cases, the epigenetic mark was found on adenosines embedded in the predicted A/G-methylated A-C sequence. (pharmaceuticalintelligence.com)
  • Long non-coding RNAs (lncRNAs) are widely recognized as important regulators of gene expression. (mdpi.com)
  • The second group includes rRNAs and long non-coding RNAs (lncRNAs), which to date are very poorly functionally annotated. (biomedcentral.com)
  • LncRNAs are defined as transcripts of more than 200 nucleotides in length not containing an extended open reading frame. (biomedcentral.com)
  • In-depth analysis revealed that lncRNAs is an extensive and very heterogenic group from mRNA-like transcripts to circular RNAs. (biomedcentral.com)
  • According to the GENCODE project one third of all human lncRNAs genes are primate-specific [ 4 ]. (biomedcentral.com)
  • LncRNAs share a lot of common features with mRNAs, they are often capped, polyadenylated and undergo splicing. (biomedcentral.com)
  • Whereas 95% of all multi-exon mRNAs are alternatively spliced only 25% of lncRNAs undergo alternative splicing. (biomedcentral.com)
  • For example, XIST (X-inactive specific transcript), one of the first described lncRNAs has a low level of sequence conservation, but a highly conserved function across placental mammals - inactivation of the X chromosome [ 5 ]. (biomedcentral.com)
  • These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. (antibodies-online.com)
  • Through recognition of its binding proteins, m6A can regulate various aspects of mRNA metabolism and is essential for maintaining higher brain functions. (encyclopedia.pub)
  • In Vertebrates, Nanos paralogous genes were associated to partial redundancies and specific functional evolutions. (nature.com)
  • In teleostean fish, two nanos1 genes were generated, probably during the Teleost specific whole genome duplication (3R). (nature.com)
  • In Paper I , we describe a protocol for transcript profiling of single cells, capable of measuring the relative expression levels for genes of interest. (kth.se)
  • Although the FPPE samples yielded fewer transcripts and genes compared to FF, there was a high agreement in gene expression between paired anatomical areas for FFPE and FF samples. (kth.se)
  • When comparing the transcriptomes across developmental stages, we found an over-representation of genes involved in growth regulation in the early development stage in C. virgata . (frontiersin.org)
  • Analyzing the human transcriptome in this way, we identified more than 12,000 methylated sites in mRNA molecules derived from approximately 7,000 protein-coding genes. (pharmaceuticalintelligence.com)
  • The transcripts of most expressed genes, in a variety of cell types, were shown to be methylated, indicating that m6A modifications are widespread. (pharmaceuticalintelligence.com)
  • Thousands of long non-coding RNA (lncRNA) genes are annotated in the human genome. (biomedcentral.com)
  • Only two Bcl-2 family genes have been found in Drosophila melanogaster including the pro-cell survival, human Bok-related orthologue, Buffy. (sdbonline.org)
  • To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
  • Experimentally confirmed target genes were identified for the 73 diagnostic miRNAs, from which proliferation genes were selected from CRISPR-Cas9/RNA interference (RNAi) screening assays. (cdc.gov)
  • Pansensitive and panresistant genes to 21 NCCN-recommended drugs with concordant mRNA and protein expression were identified. (cdc.gov)
  • We use these engineered Cas9 activation complexes to investigate sgRNA targeting rules for effective transcriptional activation, demonstrate multiplexed activation of 10 genes simultaneously, and upregulate long intergenic non-coding RNA (lincRNA) transcripts. (cdc.gov)
  • Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. (antibodies-online.com)
  • We also predicted phasing of siRNAs, which are regulated by the RNA interference (RNAi) pathway. (uni-frankfurt.de)
  • Analysis revealed that Buffy acted to rescue the associated loss of locomotor ability observed in the alpha-synuclein-induced model of PD, while Buffy RNA interference resulted in an enhanced alpha-synuclein-induced loss of climbing ability. (sdbonline.org)
  • To date, various genome-scale loss-of-function screening methods have been developed, including approaches employing RNA interference 1 , 2 and the RNA-guided endonuclease Cas9 from the microbial CRISPR (clustered regularly interspaced short palindromic repeat) adaptive immune system 3 , 4 . (cdc.gov)
  • The Stellaris® RNA FISH technology from LGC Biosearch Technologies offers an RNA visualisation method for simultaneous detection, localisation, and quantification of individual mRNA molecules at the cellular level. (bioscience.co.uk)
  • When bound to RNA, a fluorescent signal is produced for the detection of single RNA molecules as diffraction-limited spots using conventional fluorescence microscopy. (bioscience.co.uk)
  • In the case of RNA molecules that bind target RNA segments, such as microRNAs (miRNAs), the altered base pairing can change binding specificities. (biomedcentral.com)
  • Modifications to RNA molecules are much more common and are critical for regulating diverse biological processes. (pharmaceuticalintelligence.com)
  • The different building blocks can affect the complementarity of the RNA molecules, alter their structure, and enable the binding of specific proteins that mediate various biochemical and cellular outcomes. (pharmaceuticalintelligence.com)
  • Identified in bacterial rRNAs and tRNAs as early as the 1950s, this type of methylation was subsequently found in other RNA molecules, including mRNA, in animal and plant cells as well. (pharmaceuticalintelligence.com)
  • Epitranscriptomics, also known as RNA modifications, refers to the study of post-transcriptional modifications of RNA molecules. (encyclopedia.pub)
  • The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). (antibodies-online.com)
  • Its M9 domain acts as both a nuclear localization and nuclear export signal. (antibodies-online.com)
  • Here we report a class of mRNA granules in human neuronal processes that are enriched in the nuclear cap-binding protein complex (CBC) and exon junction complex (EJC) core components, Y14 and eIF4AIII. (frontiersin.org)
  • The existence of mRNAs retaining both nuclear cap binding protein and EJC in the distal sites of neuronal processes suggests that some localized mRNAs have not yet undergone the "very first translation," which contribute to the spatio-temporal regulation of gene expression. (frontiersin.org)
  • We have used in situ hybridization and immunocytochemistry to compare the nuclear localization of a specific nascent pre-mRNA and the essential non-snRNP splicing factor SC-35. (northwestern.edu)
  • By using high-voltage electron microscopy, we have found that the c-fos path extends out and comes into direct contact with the nuclear envelope. (northwestern.edu)
  • We have also compared the localization of c-fos transcripts with the speckled nuclear regions that are enriched in snRNPs and the non-snRNP splicing factor SC-35. (northwestern.edu)
  • Direct observations of three-dimensional rotations have revealed a close association between the c-fos transcripts and the nuclear speckles. (northwestern.edu)
  • This study demonstrates a direct link between specific nascent RNA transcripts and nuclear speckles that are enriched in pre-mRNA splicing factors. (northwestern.edu)
  • Spector, David L. / Nascent pre-mRNA transcripts are associated with nuclear regions enriched in splicing factors . (northwestern.edu)
  • Recently, we focused the movable properties of cyclodextrin-based polyrotaxanes (PRXs), which consist of numerous cyclodextrins threaded onto the axile molecule with bulky endcaps at both ends of the axile molecule, and developed aminated PRXs as multistep transformable carriers for Cas9 ribonucleoprotein, ensuring efficient complexation, cellular internalization, endosomal escape, release, and nuclear localization. (bvsalud.org)
  • Nuclear mRNA transcripts acquire a protein coat composed of cap- and RNA-binding proteins that allow nuclear export and dictate the functional program of cytoplasmic mRNAs ( Moore, 2005 ). (rupress.org)
  • The primary function of SMN is assembling a ring of Sm proteins around small nuclear RNA (snRNA) in an early, cytoplasmic stage of small nuclear ribonucleoprotein (snRNP) biogenesis, a process essential in eukaryotes. (st-andrews.ac.uk)
  • In addition, RNA modifications have been characterized not only in abundant non-coding RNA, such as transfer RNA (tRNA), ribosomal RNA (rRNA), and small nuclear RNA (snRNAs), but also in messenger RNA (mRNA) [ 2 ] . (encyclopedia.pub)
  • Generally, lncRNA has a lower expression level, their expression is more tissue-specific, and the majority of lncRNA has nuclear localization. (biomedcentral.com)
  • Cytoplasmic RNA granules in germ cells (polar and germinal granules), somatic cells (stress granules and processing bodies), and neurons (neuronal granules) have emerged as important players in the posttranscriptional regulation of gene expression. (rupress.org)
  • Eukaryotic RNA transcripts can undergo a range of post-transcriptional modifications, which increase the diversity of the transcriptome without requiring increases in genome size. (biomedcentral.com)
  • The post-transcriptional modifications of mRNA, which include N6-methyladenosine (m6A), N1-methyladenosine (m1A), 5-methylcytidine (m5C), N7-methylguanosine (m7G), and N6,2-O-dimethyladenosine (m6Am) add a new layer to regulating mRNA metabolism and gene expression [ 2 ] . (encyclopedia.pub)
  • They can bind to the 3′ untranslated region (3′UTR) of target messenger RNAs (mRNAs) in order to regulate gene expression post-transcriptionally with no sequence specificity but by potential electrostatic interactions with the phosphate backbone of RNA 2 , 3 . (nature.com)
  • Alternative splicing of gene can generate multiple transcripts and proteins to regulate tissue and organ development [ 17 ]. (hindawi.com)
  • Different classes of RNA granules share some protein components and may use similar mechanisms to regulate mRNA translation/decay. (rupress.org)
  • The subcellular localization of EZH2 protein was predicted by using different predictors (CELLO, Euk-mPLoc, WoLF PSORT, and TargetP). (hindawi.com)
  • its subcellular localization in the initial parasite mitochondrion adjustments based on the cell routine stage. (healthandwellnesssource.org)
  • Conclusions The dynamics of Tc38 subcellular localization observed during the cell cycle and life Isosilybin phases support a major part for Tc38 related to kDNA replication and maintenance. (healthandwellnesssource.org)
  • The protein has a preference for binding single stranded DNA and RNA. (idhinhibitor.com)
  • This class of noncoding RNAs is small, single stranded, and 19-25 nucleotide long that act as negative regulators involved in posttranscriptional silencing of the gene expression [ 5 ]. (hindawi.com)
  • The National Human Genome Research Institute defines a "probe" in genetics as a "single-stranded sequence of DNA or RNA used to search for its complementary sequences in a sample genome . (darkdaily.com)
  • ADAR3 contains an arginine-rich domain, shown in pink, which binds single-stranded RNA. (biomedcentral.com)
  • Previous Cold Spring Harbor Symposia have addressed many different aspects of RNA biology such as Mechanisms of Transcription (1998), The Ribosome (2001), Epigenetics (2004) and Regulatory RNAs (2006). (cshlpress.com)
  • Consistent with this finding, core components of EJC (eIF4AIII, Barentsz, Tsunagi, and Mago nashi) are critical for oskar mRNA localization ( Hachet and Ephrussi, 2001 , 2004 ). (frontiersin.org)
  • We review the relationship between different classes of these granules and discuss how spatial organization regulates messenger RNA translation/decay. (rupress.org)
  • RNA's multitasking prowess, at the heart of the RNA World hypothesis implicating RNA as the first molecule of life, likely spurred the evolution of numerous modified nucleotides. (pharmaceuticalintelligence.com)
  • Five hundred ninety-two nucleotides compared to 2453 for mRNA. (biomedcentral.com)
  • In Paper III , we present a protocol for performing spatial mRNA genome-wide expression profiling of FFPE tissue specimens. (kth.se)
  • Further, we developed a pipeline to characterise novel genome-wide endogenous short interfering RNAs (endo-siRNAs). (uni-frankfurt.de)
  • The molecular link between splicing and mRNA localization/local translation has also been demonstrated in mammalian neurons. (frontiersin.org)
  • It is the most abundant RNA modification, with approximately 25% of mammalian messenger RNAs (mRNA) bearing the mark [ 3 ] . (encyclopedia.pub)
  • Proximity labeling has also been used to find interaction partners of heterodimeric protein phosphatases, of the miRISC (microRNA-induced silencing complex) protein Ago2, and of ribonucleoproteins. (wikipedia.org)
  • These results validated a different transcript in pigs and characterized its expression profile in fetal tissues of different gestation stages, which indicated that EZH2 played important roles during porcine embryonic development. (hindawi.com)
  • Considering the varieties of EZH2 splicing variant, identifying its transcript in porcine fetal tissues is the foundation to study its function. (hindawi.com)
  • Moreover, none of the EZH2 transcript variants have yet been identified in porcine fetal tissues. (hindawi.com)
  • In this study, we identified a transcript variant of EZH2 in porcine fetal tissues by cloning and sequencing. (hindawi.com)
  • We found RCC tissues displayed significantly higher miR-34a expression level than their corresponding noncancerous tissues, particularly in chromophobic subtype. (hindawi.com)
  • MIR-34A has its own transcript and is expressed at higher levels than MIR-34B/C in most tissues, and this expression could be dysregulated in multiple diseases, especially in cancers [ 11 ]. (hindawi.com)
  • ETS-1 and ETS-2 mRNA and protein levels were much higher in the ocular tissues of Tyrp-1-TAg mice than in control ocular tissues from wild-type mice. (molvis.org)
  • A-to-I editing, which is catalyzed by enzymes of the adenosine deaminase acting on RNA (ADAR) family, is most prevalent in the central nervous system (CNS) but occurs in many tissues [ 1 - 3 ]. (biomedcentral.com)
  • Germ cells contain other granules that may harbor translationally silenced mRNAs important for the development of other early embryonic tissues ( Navarro and Blackwell, 2005 ). (rupress.org)
  • In fact this protein (then named Isosilybin TbRBP38) and the Leishmania tarentolae orthologue (LtRBP38) were proposed as mitochondrial RNA binding proteins involved in non-specific modulation of mitochondrial RNA stability [11]. (healthandwellnesssource.org)
  • Notably, Debcl modulates Drp1 mitochondrial localization during apoptosis. (sdbonline.org)
  • We developed a generic automated eukaryotic short interfering RNA (siRNA) analysis tool, called RAPID. (uni-frankfurt.de)
  • Among the 140 modified RNA nucleotide variants identified, methylation of adenosine at the N6 position (m6A) is the most prevalent epigenetic mark in eukaryotic mRNA. (pharmaceuticalintelligence.com)
  • An antibody prepared against a peptide sequence specific to the Aedes aegypti GPI-linked carbonic anhydrase labels plasma membranes of a number of cell types including neuronal cells and muscles. (silverchair.com)
  • We noticed elevated immunofluorescence strength from the 18 antibody staining, which can be specific to triggered -catenin conformation under tensile stress, in ZO-1,-2 DKO cells (Supplementary Fig.?S1a, b). (aboutsciencenow.info)
  • To date, a lot of EZH2 variants have been found in various cell and tissue types [ 18 - 20 ]. (hindawi.com)
  • Their design of molecular probes that target the genomic sequences of specific pathogens can enable diagnosticians and clinical laboratories to spot extremely small amounts of viral and bacterial pathogens in patients' biological samples, as well as in the environment and wildlife. (darkdaily.com)
  • The (PCR) technique relies on primers that bind to nucleic acid sequences specific to an organism or group of organisms. (darkdaily.com)
  • However, it is difficult to capture the complexity of transcript isoform variance using these libraries, and large cDNA sequences are often difficult to clone into size-limited viral expression vectors. (cdc.gov)
  • Given its diverse roles in mRNA metabolism and gene regulation, altered m6A profiles have been linked to various illnesses, including cancers and psychiatric disorders [ 4 ] . (encyclopedia.pub)
  • The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. (antibodies-online.com)
  • Through this interaction, mRNAs and their associative proteins form messenger ribonucleoprotein particles (mRNPs) that are actively transported along the cytoskeleton to intracellular destinations. (frontiersin.org)
  • RNA modifications known as epitranscriptomics have emerged as a novel layer of transcriptomic regulation. (encyclopedia.pub)
  • Among the hundreds of known RNA modifications, N6-methyladenosine (m6A) is the most abundant mRNA modification in eukaryotes. (encyclopedia.pub)
  • Although chemical modifications of RNA have been described for a half-century, only recently with the advancement of technology have we started to elucidate their functions [ 1 ] . (encyclopedia.pub)
  • Among them, m6A is one of the most abundant modifications of the mRNA in eukaryotes and the best-studied modification so far [ 3 ] . (encyclopedia.pub)
  • This showed that non-coding RNAs are a lot more abundant than it was considered before. (biomedcentral.com)
  • Human ZAP inhibits many viruses, including HIV and coronaviruses, by binding to viral RNAs to promote their degradation and/or translation suppression. (bvsalud.org)
  • The protein functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. (idhinhibitor.com)
  • The 84th Cold Spring Harbor Symposium focused on RNA Control & Regulation and provided a current synthesis of the enormous progress in our understanding of RNA's influence on cells and organisms and how, when aberrant, its effects may contribute to the progression of disease. (cshlpress.com)
  • Thus, GCGs contain proteins involved in translation initiation, translation control, and mRNA decay, which is consistent with their proposed role in the regulation ofs maternal mRNA expression. (rupress.org)
  • Although the current generation of dCas9-based transcription activators is able to achieve up-regulation of some endogenous loci, the magnitude of transcriptional up-regulation achieved by individual single-guide RNAs (sgRNAs) 12 typically ranges from low to ineffective 8 - 11 . (cdc.gov)
  • To analyze the regulatory mechanism involved in the high growth potential of C. virgata , we performed RNA-seq-based transcriptome analysis and illustrated a comprehensive gene expression map of the species. (frontiersin.org)
  • Through de novo transcriptome assembly with the RNA-seq reads from whole organ samples of C. virgata at the germination stage (2 days after germination, DAG), early young development stage (8 DAG), young development stage (17 DAG), and adult development stage (28 DAG), we identified 21,589 unified transcripts (contigs) and found that 19,346 and 18,156 protein-coding transcripts were homologous to those in rice and Arabidopsis , respectively. (frontiersin.org)
  • We find that the small RNA transcriptome is altered, even if a gene unrelated to RNAi pathway is targeted. (uni-frankfurt.de)
  • The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. (antibodies-online.com)
  • Both highly sensitive and specific, a Stellaris RNA FISH assay is comprised of up to 48 single-labelled oligonucleotides designed to selectively bind to target RNA transcripts. (bioscience.co.uk)
  • The few dozens of maxicircles carry information equivalent to that of the mtDNA from higher eukaryotes while the several thousand varied minicircles carry info for RNA editing in the form of guidebook RNA (gRNAs) that direct extensive modification of the maxicircle mRNA transcripts [1]. (healthandwellnesssource.org)
  • Heterogeneous population of RNA granules serve as motile units to translocate, store, translate, and degrade mRNAs in the dendrites contain cis -elements and trans -acting factors such as RNA-binding proteins and microRNAs to convey stimulus-, transcript-specific local translation. (frontiersin.org)
  • The results imply that, in addition to PAL, other lignin-specific enzymes and/or tissue structural changes may have more significant roles in tissue toughening of broccoli branchlets. (scialert.net)
  • Nanos are RNA-binding proteins playing crucial roles in germ cell development and maintenance. (nature.com)
  • RNA transport and regulated local translation play critically important roles in spatially restricting gene expression in neurons. (frontiersin.org)
  • Furthermore, we found homogenous and recurrent iCNV profiles in the high-grade tumors of relapse patients and identified expression of gene SPP1 in the tumor stroma as a potential prognostic mRNA marker in pediatric brain tumor relapse patients. (kth.se)
  • however, no independent prognostic value was found for any of the markers. (cancerindex.org)
  • As a result, ADAR-mediated editing can post-transcriptionally alter codons, introduce or remove splice sites, or affect the base pairing of the RNA molecule with itself or with other RNAs. (biomedcentral.com)
  • In Paper II , we present an assay for spatial RNA quality evaluation, used to estimate the success for tissue specimens before proceeding with more expensive spatial sequencing methods. (kth.se)
  • We showed that the method is capable of measuring high RNA quality in tissue areas of both high and low cell density and that the spatial RNA integrity patterns are reflected in spatial transcriptomics data. (kth.se)
  • In Paper IV , we present an approach to investigate in situ transcript derivedinferred copy number variation (iCNV) profiles based on spatial transcriptomics data. (kth.se)
  • Microglia cells have been implicated to play an important role in the tumor microenvironment, and we found spatial co-localization of microglia and epithelial-to-mesenchymal transition (EMT) signatures in our patient cohort. (kth.se)
  • In 2019, Alice Ting and the Ting lab have used APEX to identify RNA localized to specific cellular compartments. (wikipedia.org)
  • Transcriptomic analysis of ZAP-isoform-specific knockout cells revealed uncharacterized host mRNAs targeted by ZAPL/S with broad cellular functions such as unfolded protein response (UPR), epithelial-mesenchymal transition (EMT), and innate immunity. (bvsalud.org)
  • Overall, this study demonstrates that the competition between splicing and IPA is a potential target for the modulation of the ZAPL/S balance, and reports new cellular transcripts and processes regulated by the ZAP isoforms. (bvsalud.org)
  • This enabled the diversified complementarity and secondary structures that allow RNA species to specifically interact with other components of the cellular machinery such as DNA and proteins. (pharmaceuticalintelligence.com)
  • In both cases, a subset of translationally silenced mRNAs is packaged into RNP granules that lack a limiting membrane and are visible using light microscopy. (rupress.org)
  • A-to-I RNA editing is a post-transcriptional modification that converts adenosines to inosines in both coding and noncoding RNA transcripts. (biomedcentral.com)
  • Although the identification of m6A in RNA is 40 years old, until recently researchers lacked efficient molecular mapping and quantification methods to fully understand the functional implications of the modification. (pharmaceuticalintelligence.com)
  • Further, using RAPID, we investigated the aberrations of endo-siRNAs, and their respective transcriptomic alterations caused by an RNAi pathway triggered by feeding small RNAs against a target gene. (uni-frankfurt.de)
  • In efforts to identify pharmacological agents that target this pathway, we found that fisetin, a natural flavonoid, is an ULK1 inhibitor that decreases type I IFN-induced dACE2 expression. (bvsalud.org)
  • It is catalyzed by ADAR (adenosine deaminase acting on RNA) enzymes, which exist throughout the body but are most prevalent in the central nervous system. (biomedcentral.com)
  • In mammals, the most prevalent form of RNA editing involves the conversion of adenosine to inosine (A-to-I) by hydrolytic deamination at the C6 position of adenine (Figure 1 a) [ 1 ]. (biomedcentral.com)
  • We established that endogenous ZAPL and ZAPS localize to membrane compartments and cytosol, respectively, and that the differential localization correlates with their target-RNA specificity. (bvsalud.org)
  • Cooperative activity of GR with other transcription factors and a plethora of coregulators contribute to the tissue- and context-specific response toward the endogenous and pharmacological glucocorticoids (GCs). (uni-ulm.de)
  • Our goal is to convey a picture of pattern formation that draws attention to the principles rather than solely to specific molecular mechanisms. (mpi-cbg.de)
  • Studies conducted on PRPF31 mRNA levels in lymphoblast cell lines isolated from family members with either deletion or splice site mutations indicate that penetrance requires the co-inheritance of a low expressing wild-type (WT) allele alongside the mutant allele [ 13 , 14 ], whereas the presence of a high expressing WT allele is protective. (molvis.org)
  • GCGs direct the timing of maternal mRNA translation to promote germ cell development in the early embryo and establish the germ line for the next generation. (rupress.org)
  • Subsequently, we detected the expression of EZH2 on mRNA level and protein level in two different embryonic development stages (65-dpc and 90-dpc) via qRT-PCR and western blots. (hindawi.com)
  • Profiling 2 to 4 single cells per patient and measuring gene-specific expression from targets previously associated with metastatic breast cancer supports the use of our protocol as a diagnostic tool. (kth.se)
  • However, the transcript variant and temporal expression pattern of EZH2 in the middle and late porcine fetus are still unknown. (hindawi.com)
  • Furthermore, the decreased expression of the EZH2 gene is crucial for stem cell differentiation into specific cell lineages involved in myogenesis, adipogenesis, osteogenesis, neurogenesis, and haematopoiesis [ 9 ]. (hindawi.com)
  • However, the transcript and expression status of the EZH2 gene during porcine embryonic development remains unknown. (hindawi.com)
  • We found that as mice aged, Rac1 expression declined in the brain. (bvsalud.org)
  • Upon evaluating different feature sets, we found that genic features are as predictive, of gene expression, as the epigenetic features. (uni-frankfurt.de)
  • This specific phenotype also was caused by the expression of titin A168-170. (rupress.org)
  • These analyses of the relationships between patient survival and expression patterns obtained from an open mRNA database in cancer patients indicate that ORs may be cancer biomarkers and therapeutic targets. (bmbreports.org)
  • In addition, OR expression pattern in various cancers were analyzed based on RNA-sequencing data reported in the Cancer Genome Atlas (TCGA), and the associations between patient survival outcomes and OR levels were analyzed to determine the clinical relevance and significances of OR expression in tumors. (bmbreports.org)
  • DEFB1 expression after transfection with two micro RNAs (miRNAs), hsa-miR-186-5p and hsa-miR-340-5p, provided evidence that DEFB1 expression could be modulated by these miRNAs and hsa-miR-186-5p had a higher binding efficiency with DEFB1. (cdc.gov)
  • In 2019, BioID has been tethered to the beta-actin mRNA transcript to study its localization dynamics. (wikipedia.org)
  • Our tool captures diverse siRNA characteristics from small RNA sequencing data and provides easily navigable visualisations. (uni-frankfurt.de)
  • It is also thought have a primary role in the formation of specific myometrial protein species in parturition. (antibodies-online.com)
  • Find HNRNPA1 ELISA Kits for a variety of species such as anti-Mouse HNRNPA1, anti-Rat HNRNPA1, anti-Chicken HNRNPA1. (antibodies-online.com)
  • 2004). Moreover, PAL activity was found to be enhanced in several plant species in response to wounding imposed by either harvest or other stresses during postharvest handling as observed in newly harvested asparagus spears (Bhowmik et al . (scialert.net)
  • Multiple transcript variants encoding different isoforms have been found for this gene. (idhinhibitor.com)
  • RNA editing refers to post-transcriptional processes that alter the nucleotide sequence of an RNA transcript by insertion, deletion or nucleotide conversion. (biomedcentral.com)
  • In the central dogma RNA acts as a simple intermediary between the DNA that carries the genetic information and the proteins that define the whole variety of biological processes in the cell. (biomedcentral.com)
  • NCDN was found to not associate with snRNPs, indicating an snRNP-independent interaction with SMN. (st-andrews.ac.uk)
  • This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. (antibodies-online.com)
  • Prior to serum induction no c-fos RNA is detected, but these transcripts localize to two dots in the interphase nucleus after induction. (northwestern.edu)
  • How each neuron finds its synaptic partners has been a central question in developmental neurobiology. (stanford.edu)
  • Although RNA editing has long been considered a relatively rare processing event, more recent research suggests that the vast majority of pre-mRNAs are edited [ 6 ]. (biomedcentral.com)
  • This review is focused on hereditary diseases in the pathogenesis of which long non-coding RNAs play an important role. (biomedcentral.com)
  • Based on the length of the transcript non-coding RNAs are divided into two groups: short and long non-coding RNAs. (biomedcentral.com)