Mouse Embryo Fibroblast Cells with Texas Red-X, Alexa Fluor 488, and DAPI - In this section, a Swiss mouse embryo fibroblast cell is presented that was resident in a culture labeled with the fluorophore Texas Red-X conjugated to wheat germ agglutinin, a fluorescent lectin that selectively binds to sialic acid residues. (fsu.edu)
Directly following antibody binding, the chromatin is digested and NGS libraries are prepared in a single step by tagmentation using the protein A-Tn5 (pA-Tn5) transposome enzyme that has been pre-loaded with sequencing adapters. (epigenie.com)
In this study, we investigated the immunomodulatory effects of a recombinant tegumental calcium-binding EF-hand protein 4 of F. gigantica (rFg-CaBP4) on goat monocytes. (biomedcentral.com)
Both the cyclooxygenase and the peroxidase active sites are located in the catalytic domain, which accounts for approximately 80% of the protein. (wikipedia.org)
Reduced nuclear localization of the transcription factor Msn2 and lower glycogen accumulation indicated higher protein kinase A (cAMP-dependent protein kinase, PKA) activity in many light-sensitive gene deletion strains. (biomedcentral.com)
The high mobility of hydration water ensures that all thermally activated processes at the protein-water interface, such as binding, recognition and catalysis, can proceed at high rates. (lu.se)
Equatorial segment protein (ESP), a novel 349-amino acid concanavalin-A-binding protein encoded by a two-exon gene ( SP-ESP ) located on chromosome 15 at q22, has been localized to the equatorial segment of ejaculated human sperm. (bioone.org)
The non-inhibitory antibody additionally effectively endocytoses through clathrin with out inducing receptor clustering however with slower lysosomal co-localization kinetics. (kashbiotech.com)
Myosin2
In Saccharomyces cerevisiae , the myosin V motor Myo2 binds the vacuole-specific adapter Vac17 to attach to the vacuole/lysosome and initiate transport. (rupress.org)
The experiments show that WASP, myosin and WIP are recruited to sites where endocytosis is about to occur through specific interactions with other proteins. (elifesciences.org)
Analyze1
To analyze these images we have used an in-house script to browse manually and assign localization rapidly and efficiently. (weizmann.ac.il)
Inhibits1
In addition, deoxyadenosine irreversibly binds to and inhibits S-adenosylhomocysteine hydrolase, which also contributes to abnormal DNA synthesis [ 11,12 ]. (medilib.ir)
Covalent1
1984. Covalent binding of benzidine to rat hemoglobin [Abstract]. (cdc.gov)
Receptor2
In addition, we show that both mPR beta and PGMRC1, two members of distinct membrane-bound progestin receptor classes, exhibit highly similar ovarian expression profiles during the reproductive cycle with maximum levels during vitellogenesis and a down-expression during late vitellogenesis. (biomedcentral.com)
As well as, DLL4 ligand binding to the NOTCH3 receptor mediates transendocytosis of NOTCH3-ADCs into ligand-expressing cells. (kashbiotech.com)
Antibody3
Unlike MNase-Seq or ATAC-Seq methods that target open chromatin and are therefore dependent on chromatin accessibility, CUT&Tag utilizes an antibody-based enzyme tethering strategy to target specific histone modifications or proteins to reveal chromatin-binding information that is specific to those sites or proteins of interest. (epigenie.com)
Instead of the sonication of fixed chromatin and immunoprecipitation steps performed in ChIP-Seq protocols, in CUT&Tag, fresh (not frozen) unfixed cells are bound to concanavalin A beads and the antibody incubation is performed with cells in their native state. (epigenie.com)
Right here, we report Fc-binding antibody-recruiting molecules (Fc-ARMs) as the primary instance addressing this subject. (kashbiotech.com)
Quantitative1
Here we used genetics and quantitative live-cell imaging to probe the mechanisms that concentrate NPFs at endocytic sites, and to investigate how NPFs regulate actin assembly onset. (elifesciences.org)
Enzymes2
PTGS1 (COX-1) and PTGS2 (COX-2) are bifunctional enzymes that carry out two consecutive chemical reactions in spatially distinct but mechanistically coupled active sites. (wikipedia.org)
AEPs are widely distributed in land plants, where they are also sometimes called vacuolar processing enzymes due to their localisation in vacuoles and their role in processing vacuolar proteins [ 1 , 5 ]. (silverchair.com)
Intracellular1
Mechanistically, intracellular mature IL-37 directly bound to the CAAX motif in the C-terminal hypervariable region of Rac1, and then inhibited Rac1 membrane translocation and subsequent downstream signaling. (nature.com)
Motif1
Our results demonstrate that SH3 (Src homology 3) domain-PRM (proline-rich motif) interactions involving multivalent linker proteins play central roles in concentrating NPFs at endocytic sites. (elifesciences.org)
Affinity2
A glycoprotein albumin from hen's egg white with strong iron-binding affinity. (lookformedical.com)
Fc-ARMs are composed of an Fc-binding peptide and a concentrating on ligand , enabling the exploitation of endogenous antibodies via fixed affinity to the Fc area of antibodies, whose sequence is conserved in distinction to the Fab area. (kashbiotech.com)
Enzyme2
Each monomer of the enzyme has a peroxidase and a PTGS (COX) active site. (wikipedia.org)
Alternative sites for cell density regulation of the enzyme could rely on its sensitivity to sulfhydryl groups. (embl.de)
Investigate1
Cleavage Under Targets and Tagmentation (CUT&Tag) is a method to investigate genomic localization of histone modifications and some transcription factors that reveals interactions between proteins and DNA or identifies DNAbinding sites for proteins of interest. (epigenie.com)
Proteins play1
These findings provide additional evidence that calcium-binding EF-hand proteins play roles in host-parasite interaction. (biomedcentral.com)
References1
In the study of the CCT complex, we have mutated Glu to Asp in the ATP binding site of subunits 3 (MA3) (See Nadler-Holy et al in References). (weizmann.ac.il)
Membrane3
the membrane-binding domain consists of a series of amphipathic α helices with several hydrophobic amino acids exposed to a membrane monolayer. (wikipedia.org)
In lower vertebrates, steroid-induced oocyte maturation is considered to involve membrane-bound progestin receptors. (biomedcentral.com)
Two totally distinct classes of putative membrane-bound progestin receptors have been reported in vertebrates. (biomedcentral.com)
Interaction5
For more than ten years after bafilomycin was discovered as a V-ATPase inhibitor, the site of its interaction with V-ATPase was unclear. (wikipedia.org)
Further narrowing bafilomycin's interaction site, they found that specific addition of just Vo subunit a could restore function. (wikipedia.org)
There is, however, no clear interaction between the nitrilotriacetate anion and the synergistic anion binding site, Arg121. (lookformedical.com)
Fungi sense visible light through the flavin-binding photoreceptor White Collar 1 (WC-1) which together with its interaction partner WC-2 acts as a light-activated transcription factor [ 3 ]. (biomedcentral.com)
K 1/2 increases as Ca 2+ rises, and this dependence is well described by a modified Michaelis-Menten function, indicating that modulation arises from the interaction of Ca 2+ with a single site without apparent cooperativity. (silverchair.com)
Data1
We additionally provide unpublished data on the localization changes as a result of the hypomorphic allele of the proteosome subunit Pup2. (weizmann.ac.il)
Adult1
The localization of CaBP4 within adult fluke structure was detected by immunohistochemical analysis. (biomedcentral.com)
Domain4
Tegumental proteins (TegPs) belong to a family of calcium-binding proteins (CaBPs), which contain two EF-hand motifs at the N-terminal domain and a C-terminal dynein light chain-like domain [ 7 ]. (biomedcentral.com)
These studies provide evidence that WASP and WIP play central roles in establishment of a robust multivalent SH3 domain-PRM network in vivo, giving actin assembly onset at endocytic sites a switch-like behavior. (elifesciences.org)
For example, a region of WASP known as the proline-rich domain can bind to proteins that contain an "SH3" domain. (elifesciences.org)
In the structure, both empty iron binding clefts are in the open conformation, lending weight to the theory that Fe(3+) binding or release in transferrin proceeds via a mechanism that involves domain opening and closure. (lookformedical.com)
Regulation2
In the present study, we report the characterization of the full coding sequence of rainbow trout PGMRC1 and mPR beta cDNAs, their tissue distribution, their ovarian expression profiles during oogenesis, their hormonal regulation in the full grown ovary and the in situ localization of PGMRC1 mRNA in the ovary. (biomedcentral.com)
These assemblies are widely used in biology as they enable multivalent binding, ultra-sensitive regulation, and compartmentalization. (springeropen.com)
Structure1
Upon Fe3+ uptake, transferrins undergo a large scale conformational transition: the apo structure with an opening of the interdomain cleft is transformed into the closed holo structure, implying initial Fe3+ binding in the open form. (lookformedical.com)
Specific2
To make proteins, such as binding, recognition and catalysis, often progress, it is therefore helpful to adopt a sceptical atti- involve specific interactions with individual water mol- tude. (lu.se)
Localization of a female-specific marker on the chromosomes of the brown seaweed Saccharina japonica using fluorescence in situ hybridization. (aatbio.com)
Effect2
Bafilomycin A1 was also shown to have an anti-proliferative effect on concanavalin-A-stimulated T cells. (wikipedia.org)
This research is undertaken to evaluate the effect of MSC-derived exosome on concanavalin-A (con-A)-induced liver injury. (biomedcentral.com)
Interest1
Our screening assay was designed to explore yeast cell biology by assessing two cellular key features of interest: subcellular localization and fluorescence intensity. (weizmann.ac.il)
Cells1
GML treated T cells have decreased cellular proliferation when stimulated with phorbol 12-myristate 12-acetate (PMA), concanavalin A (ConA), and Toxic Shock Syndrome Toxin-1 (TSST-1) 13 . (nature.com)
Active1
Second, PGG2 is reduced to PGH2 in the peroxidase active site. (wikipedia.org)
Terms1
This article is distributed under the terms of an Attribution-Noncommercial-Share Alike-No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/ ). (rupress.org)
Members1
The biochemical properties of CaBPs from different parasites have been investigated, and despite the considerable resemblance between structural models of all family members, differences exist in the dimerization, ion-binding and drug-binding characteristics of these apparently similar proteins [ 14 ]. (biomedcentral.com)
Activation1
This model explains TMEM16A activation as a sequential, direct, and V m -dependent binding of two Ca 2+ ions coupled to a V m -dependent binding of an external Cl − ion, with V m -dependent transitions between states. (emory.edu)