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  • serovar
  • C. trachomatis serovar L2 (434/Bu) was propagated within McCoy cell monolayers grown in Eagle's MEM ( Invitrogen , Grand Island, NY) supplemented with 10% FCS, 1.5 g/l sodium bicarbonate, 0.1 M nonessential amino acids, and 1 mM sodium pyruvate. (jimmunol.org)
  • We used the fast-chelating agent 2,2′-bipyridyl (BPDL) to homogeneously starve Chlamydia trachomatis serovar L2 of iron, starting at 6 or 12 h postinfection. (asm.org)
  • MOMP
  • C. trachomatis MOMP was expressed on the surface of E. coli cells (including "porin knockout" cells) after optimizing leader sequence, temperature and medium composition, and the protein was functionally reconstituted at the single-channel level to confirm it was folded correctly. (biomedcentral.com)
  • C. trachomatis MOMP was functionally expressed on the surface of E. coli cells under newly optimized conditions. (biomedcentral.com)
  • C. trachomatis VS domains, and homologous regions in MOMPs from other species, could correspond to cysteine-rich surface-exposed loops in a porin β-barrel, and EB MOMP is oxidised and highly cross-linked, making the OM very stable. (biomedcentral.com)
  • species
  • IMPORTANCE Chlamydiae are well known for their species specificity and tissue tropism as well as their association with unique diseases. (asm.org)
  • Hexagonal lattices, located between the outer and cytoplasmic EB membranes, and similar structures were present in multiple Chlamydia species. (asmscience.org)
  • pathogen
  • These responses of replicative-phase Chlamydia to iron starvation indicate a prioritization of survival over replication, enabling the pathogen to "stock the pantry" with ingredients needed for rapid growth once optimal iron levels are restored. (asm.org)
  • protein
  • Here, we used IncD as a model to investigate Inc/Inc interaction in the context of Inc protein expression in C. trachomatis . (frontiersin.org)
  • The heterologous heat shock protection for Escherichia coli , and increased HtrA during cell wall disruption via penicillin and heat shock, indicates an important role for HtrA during high protein stress conditions for Chlamydia trachomatis . (beds.ac.uk)
  • biphasic
  • Chlamydiae are characterized by a biphasic life cycle, alternating between infectious elementary bodies (EBs) and replicative reticulate bodies (RBs) ( 5 ). (asm.org)
  • genes
  • We also observed an increase in transcription of genes involved in DNA repair and neutralizing oxidative stress, indicating that Chlamydia employs an "all-or-nothing" strategy. (asm.org)
  • vaccine
  • The accumulated body of evidence has provided important guiding principles for Chlamydia vaccine development. (asmscience.org)
  • Collectively, contemporary technologies have enabled considerable progress in Chlamydia vaccine development in the recent past, but several issues remain to be addressed before advancing the identified antigens into human clinical trials. (asmscience.org)
  • Chlamydia Vaccine: Progress and Challenges, p 311-333. (asmscience.org)
  • Scheme for Chlamydia vaccine candidate identification and evaluation. (asmscience.org)
  • vacuole
  • Following endocytosis by a host cell, chlamydiae reside within a vacuole termed an inclusion, which is nonfusogenic with the endosomal/lysosomal pathway but acquires sphingomyelin and cholesterol from the Golgi apparatus ( 6 - 8 ). (asm.org)
  • mechanisms
  • However, in the "arms race" of co-evolution, Chlamydiae have developed mechanisms to promote cell viability and inhibit cell death. (frontiersin.org)
  • To maintain this replicative cellular niche, Chlamydiae have evolved mechanisms to prevent host cell death, one in particular being the inhibition of apoptosis. (frontiersin.org)
  • These findings have provided important information for further understanding C. trachomatis pathogenic mechanisms. (sigmaaldrich.com)
  • conjunctivitis
  • We found a particularly increased frequency of C. trachomatis genotype E in neonatal conjunctivitis, which may indicate an epidemiological association between this genotype and the newborn population. (biomedcentral.com)
  • Biology
  • IMPORTANCE By utilizing an experimental approach that monitors the immediate global response of Chlamydia trachomatis to iron starvation, clues to long-standing issues in Chlamydia biology are revealed, including how Chlamydia adapts to this stress. (asm.org)