Gangliosides, complex glycosphingolipids on the plasma membrane containing one or more sialic acid residues, are key components of microdomains. (jneurosci.org)
Based on the ganglio GSL series, gangliosides are synthesized, where sialic acids are linked to the glycan structure to produce negatively charged GSLs. (springer.com)
Membrane3
Zebrafish encodes several sialidases belonging to the NEU3 group, the plasma membrane-associated member of the family with high specificity toward ganglioside substrates. (unibs.it)
Membrane redistribution of gangliosides and glycosylphosphatidylinositol-anchored proteins in brain tissue sections under conditions of lipid raft isolation. (uiowa.edu)
Whereas the trisaccharidic head group of Gb 3 defines the specificity of Shiga toxin binding, the lipophilic part composed of sphingosine and different fatty acids is suggested to determine its localization within membranes impacting membrane organisation and protein binding eventually leading to protein internalisation. (springer.com)
Extracellular1
Characterization of surface markers on extracellular vesicles isolated from lymphatic exudate from patients with breast cancer. (gu.se)
Clinical3
Clinical localization to the lumbosacral plexus was supported by neurodiagnostic tests, and magnetic resonance imaging of the lumbosacral plexus showed that the nerve roots were also involved. (biomedcentral.com)
A systematic and meticulous clinical approach, supported by intelligent selection of adjunctive tests, is required for localization and diagnosis. (biomedcentral.com)
This report shows the characterization of the humoral response elicited after vaccination with 400 µg of antigen combined with 200 µg of VSSP in cancer patients representative of the Cuban real clinical practice setting. (researchsquare.com)
Sialidase6
We show that activation of Neu3 sialidase, also known as Neuraminidase-3, causing conversion of GD1a and GT1b to GM1 gangliosideM1 is an essential step in regeneration occurring in PNS (sensory) but not CNS (retinal) axons in adult rat. (jneurosci.org)
In PNS axons, axotomy activates Neu3 sialidase, increasing the ratio of GM1/GD1a and GM1/GT1b gangliosides immediately after injury in vitro and in vivo . (jneurosci.org)
Externally applied sialidase converted GD1a ganglioside to GM1 and rescued axon regeneration in CNS axons and in PNS axons after Neu3 sialidase blockade. (jneurosci.org)
Ganglioside conversion by Neu3 sialidase further activates the ERK pathway. (jneurosci.org)
In this paper, we examine Neu3 sialidase action on axonal gangliosides and resultant axon regeneration, and we assess whether these mechanisms help to explain the different regeneration capacities of CNS and PNS axons. (jneurosci.org)
Pharmacological blocking of Neu3 sialidase, downregulation of Neu3 with siRNA, or depleting gangliosides all inhibit PNS regeneration. (jneurosci.org)
20021
Over the past century, hundreds of studies have explored the mechanistic underpinnings of the taste, leading to the characterization of the umami taste receptor in 2002. (kobv.de)
Adult2
Differential distribution of major braingangliosides in the adult mouse central nervous system. (uiowa.edu)
The adult mammalian nervous system has four dominant types of gangliosides: GM1, GD1a, GD1b, and GT1b. (jneurosci.org)
Human1
Characterization of the first fully human anti-TEM1 scFv in models of solid tumor imaging and immunotoxin-based therapy. (atsbio.com)
Cell3
Its cytosolic localization was demonstrated by cell fractionation experiments. (wikipedia.org)
We aim at combining the advantages of both techniques to precisely address NP localization in the context of the cell ultrastructure. (ibecbarcelona.eu)
We studied nanocarriers (NCs) targeted to the transferrin receptor (TfR), ganglioside GM1 or ICAM1, associated to the clathrin, caveolar or cell adhesion molecule (CAM) routes, respectively. (ibecbarcelona.eu)
A lack of β-galactosidase activity leads to the massive accumulation of GM1 gangliosideM1 which results in neurodegenerative pathology. (blogspot.com)
Effects2
Effects of detergents on the redistribution of gangliosides and GPI-anchored proteins in brain tissue sections. (uiowa.edu)
The role of tumor-associated macrophages (TAMs) in cancer is often correlated with poor prognosis, even though this statement should be interpreted with care, as the effects of macrophages primarily depend on their localization within the tumor. (nutriforce.cn)
Knockout mice2
6. GMab-1, a high-affinity anti-3'-isoLM1/3',6'-isoLD1 IgG monoclonal antibody, raised in lacto-series ganglioside-defective knockout mice. (nih.gov)
In B4galnt1 knockout mice, mice with ganglioside GM1 deficiency show the characteristic symptoms of PD, such as reduced dopamine levels in the striatum, loss of dopaminergic neurons in the SNpc, aggregation of α-synuclein, and motor dysfunction. (researchsquare.com)
Cerebrosides1
We are mapping and imaging the brain's and other organs lipidome (glycolipids, cerebrosides, sphingomyelin, gangliosides and cardiolipins) and proteome in health and in pathological conditions such as blast induced traumatic brain injury, as well as mapping and imaging the anatomical distribution and localization of drugs of abuse in the addicted brain. (nih.gov)
Raft3
Our results suggest that ganglioside GM1 deficiency could compromise the neurotrophic effects and signals downstream of GDNF by altering the assembly of lipid raft membrane microdomains. (researchsquare.com)
In this review, we briefly summarize recent studies, the development of a variety of glycosphingolipid probes as well as the identification of the raft structures including gangliosides in living cells by single-molecule imaging. (bvsalud.org)
The raft marker, ganglioside GM1, is labeled with Alexa 488, while AKT is marked with Alexa 594. (ashpublications.org)
Genes1
The expression of the ganglioside synthesis-related genes B3galt4 and St3gal2 is conspicuously absent in PD patients, and the GM1 level is decreased in the substantia nigra, prefrontal cortex and various peripheral tissues [ 5 , 6 ] . (researchsquare.com)
GD1a2
In people over 60, the total amount of gangliosides in the substantia nigra of the midbrain decreases, and this reduction is most pronounced in GM1 and GD1a [ 4 ] . (researchsquare.com)
The imaging of [M-H](-) and [M-H2O-H](-) mass peaks for GD1 gangliosides showed different distribution, most likely reflecting the different spatial distribution of GD1a and GD1b species in the brain. (nih.gov)
Tumors5
4. Expression of gangliosides GD3 and 3'-isoLM1 in autopsy brains from patients with malignant tumors. (nih.gov)
7. Detection of glioma-associated gangliosides GM2, GD2, GD3, 3'-isoLM1 3',6'-isoLD1 in central nervous system tumors in vitro and in vivo using epitope-defined monoclonal antibodies. (nih.gov)
9. Gangliosides associated with primary brain tumors and their expression in cell lines established from these tumors. (nih.gov)
11. Lactotetraose series ganglioside 3',6'-isoLD1 in tumors of central nervous and other systems in vitro and in vivo. (nih.gov)
12. Gangliosides as diagnostic markers of human astrocytomas and primitive neuroectodermal tumors. (nih.gov)
Marker1
10. Sialyllactotetraosylceramide, a ganglioside marker for human malignant gliomas. (nih.gov)
Membrane1
Ganglioside-induced differentiation-associated protein 1 (GDAP1) is an integral mitochondrial outer membrane (MOM) protein, and the GDAP1 gene is one of the most abundant in missense mutations linked to CMT [ 8 - 10 ]. (biorxiv.org)
Pathological1
All these results suggest that deficiency of endogenous ganglioside GM1 may be an initiator of the pathological process of PD and play an important role in it, but the specific mechanism is not clear. (researchsquare.com)
Expression2
5. The glioma-associated gangliosides 3'-isoLM1, GD3 and GM2 show selective area expression in human glioblastoma xenografts in nude rat brains. (nih.gov)
8. Altered expression of ganglioside phenotypes of human gliomas in vivo and in vitro. (nih.gov)
Protein1
Mutations in ganglioside-induced differentiation-associated protein 1 (GDAP1) cause two types of CMT, demyelinating CMT4A and axonal CMT2K. (biorxiv.org)
Show1
Here, using GM1, GM3, and GD3 ganglioside micelles as models, we show that the sugar distributions and cationic amino acids within Aß N-terminal region modulate oligomerization of Aß temporally, and dictate the stability and maturation of oligomers. (bvsalud.org)
Mass2
19. Aberrant ganglioside composition in glioblastoma multiforme and peritumoral tissue: A mass spectrometry characterization. (nih.gov)
High-speed, single-molecule observation of these fluorescent probes revealed that gangliosides were scarcely trapped in small domains (100 nm in diameter) for more than 5 ms in steady-state cells, suggesting that rafts including gangliosides were always moving and very small. (bvsalud.org)