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  • Immunofluorescence
  • In a double immunofluorescence experiment, an adherent monolayer culture of LLC-PK1 Cells (illustrated above) was fixed, permeabilized, blocked with 10 percent normal goat serum, and treated with a cocktail of mouse anti-histones (pan) and rabbit anti-PMP 70 (peroxisomal membrane protein) primary antibodies, followed by goat anti-mouse and anti-rabbit secondary antibodies (IgG) conjugated to Texas Red and Alexa Fluor 488, respectively. (fsu.edu)
  • At the end of the treatment, the cells were fixed, and MRTF and the nuclei were visualized by immunofluorescence staining with an MRTF-specific antibody ( top ) and DAPI ( bottom ), respectively. (nih.gov)
  • Apoptosis
  • Apoptosis of cultured rat glomerular mesangial cells induced by IgG2a monoclonal anti-Thy-1 antibodi. (biomedsearch.com)
  • Renal cell apoptosis was proven by terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) stain and poly-ADP ribose polymerase expression in the unilateral ureteral obstruction (UUO) kidney. (springer.com)
  • Application of microwave-irradiation technique in deglycosylation of ginsenosides for improving apoptosis induction in human melanoma SK-MEL-2 cells. (nih.gov)
  • However, strong osmotic stress destabilizes MRTF, concomitant with apoptosis, implying that hyperosmotically induced cell death takes precedence over epithelial-myofibroblast transition, a potential consequence of MRTF-mediated phenotypic reprogramming. (nih.gov)
  • cultures
  • We show here that primary cultures of rat inner medullary collecting duct (IMCD) cells retain AQP-2 expression for at least 6 days when grown with dibutyryl cAMP (DBcAMP) supplementation. (semanticscholar.org)
  • vitro
  • None-theless, in vitro studies indicate that such alterations can have profound effects on transport systems and receptors associated with cell membranes (Levitsby et al. (springer.com)
  • Three cell lines were used as in vitro model of urolithiasis: MDCK I, MDCK II and LLC-PK1. (mdpi.com)
  • transporter
  • We present evidence for the existence of an Na(+)-dependent transporter and an Na(+)-independent transporter for polyamines in LLC-PK1 cells. (biochemj.org)
  • The cell surface molecule ABCC10 is a broad-acting transporter of xenobiotics, including cancer drugs, such as taxanes, epothilone B, and modulators of the estrogen pathway. (aacrjournals.org)
  • experiments
  • Setup is simple with ZEN imaging software, even for complex confocal live cell imaging experiments. (zeiss.com)
  • grown
  • LLC-PK1 cells were grown to confluence on coverslips, serum deprived for 3 h, and preincubated with isotonic Na + medium (15 min). (nih.gov)
  • susceptible
  • Silencing or pharmacological inhibition of MRTF prevents the osmotic stimulation of CArG-dependent transcription and renders the cells susceptible to osmotic shock-induced structural damage. (nih.gov)
  • vivo
  • Cytochalasin D inhibits ex vivo alveologenesis, indicating that cell migration is dominant driver, not cell proliferation. (uberflip.com)
  • expression
  • Temporal changes in the microfilament system of the LLC-PK1 cells were studied during differentiation in culture as defined by spectrin expression and F-actin architecture. (cshl.edu)
  • Collectively these results demonstrate that epithelial cells express a complex pattern of TM isoforms, which exhibit differential localizations within the cells and different patterns of expression depending on their origin and stage of differentiation. (cshl.edu)
  • Recent work has suggested that ABCC10 expression level is elevated in non-small cell lung cancer (NSCLC) in relation to normal lung, with ABCC10 expression in adenocarcinoma correlated with tumor grade and stage ( 5 ). (aacrjournals.org)
  • Western blot analysis indicates the expression of rOat3, but not rOat1, on the CP, and immunohistochemical staining shows that rOat3 is localized on the brush border membrane of the choroid epithelial cells. (aspetjournals.org)
  • ER stress-associated proapoptotic signals, including B-cell chronic lymphocytic leukemia (CLL)/lymphoma 2-associated × protein (BAX) expression, caspase-12 and c-Jun N-terminal kinase (JNK) phosphorylation, were activated in the UUO kidney. (springer.com)
  • Positive correlation of SOD and OPN expression as well as GSH concentration was observed in all groups of cells. (mdpi.com)
  • Decreased expression is observed in activated resting T and B cells (Takayanagi et al. (atlasgeneticsoncology.org)
  • biology
  • To give you confidence in the health of your cells every step of the way, we've highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. (thermofisher.com)
  • inhibits
  • A report demonstrated that a peptide derived from ABCC10 binds to HLA-E and inhibits NK cell-mediated lysis in a CD94 and class I-dependent fashion (Wooden et al. (atlasgeneticsoncology.org)
  • effects
  • Effects of fermented black ginseng on wound healing mediated by angiogenesis through the mitogen-activated protein kinase pathway in human umbilical vein endothelial cells. (nih.gov)
  • high
  • Abcc10 loss promotes loss of white blood cells, increased weight loss and increased lethality in mice exposed to high doses of paclitaxel (Hopper-Borge et al. (atlasgeneticsoncology.org)
  • human
  • In cell lines: In terms of cancer, the ABCC10 gene is expressed in human tumor cell lines, HepG2, CWR22RV1 and TSU-PR1 at greater or equal levels than ABCC1 , ABCC2 and ABCC3 . (atlasgeneticsoncology.org)
  • function
  • Keratin filament networks function chiefly in the endowment of epithelial cells with the resilience to withstand the mechanical stress that they may incur incidentally. (fsu.edu)
  • compounds
  • Bioactivity-based analysis and chemical characterization of anti-inflammatory compounds from Curcuma zedoaria rhizomes using LPS-stimulated RAW264.7 cells. (nih.gov)