Living mammalian cell. Medical search. Frequent questions

In the liver, cholesterol and plant sterols may be transported to peripheral tissues by very low-density lipoprotein (VLDL) and low-density lipoprotein (LDL), converted to bile acids, or transported out of the liver into the bile for excretion. (medscape.com)
In peripheral tissues, the ABC1 transporter (defective in Tangier disease) delivers cholesterol to high-density lipoprotein (HDL) for return to the liver. (medscape.com)
Collectively, our data demonstrated parathyroid tissues and cells loaded in gelatin scaffolds and prepared as double-layered microbeads through alginate hydrogel encapsulation, possessing optimal cell proliferation rate and calcium-responsive PTH secretion capability. (ncl.edu.tw)
However, one of the challenges in AAV-mediated gene therapy is achieving efficient targeting of specific cell types and tissues. (lu.se)
By introducing peptide insert modifications into the AAV capsid, the viral vectors can be engineered to target specific cell types and tissues. (lu.se)
However, a systematic approach is needed to identify the most efficient AAV variants for different cell types, tissue types, and specific targets while avoiding off-target effects, particularly in non-target tissues such as the liver. (lu.se)
Accumulated data, covering diverse cells, tissues and cohorts across the lifespan necessitates the establishment of an open and integrated database. (bvsalud.org)
It is primarily intended for measurements of activities of living cells, tissues and small animals. (lu.se)
Anaplerotic roles of pyruvate carboxylase in mammalian tissues. (medlineplus.gov)
COX-1 is expressed constitutively and is isolated throughout most cell lines in almost all mammalian tissues. (medscape.com)

Key in vitro gene mutation studies in mammalian cells were identified. (europa.eu)
We published one of the first massively parallel single-nucleus RNA-Seq (snRNA-Seq) studies in mammalian hearts. (upenn.edu)

To test the reliability of this detection scheme, we used it to probe the interactions among three mRNA-binding proteins in both fixed and living cells and compared the results to those obtained by pull-down assays. (hal.science)
Timely results for microbial bioburden monitoring of in-process cell culture samples are critical to the production process for recombinant proteins and other biopharmaceutical products. (europeanpharmaceuticalreview.com)
Once proteins, larger molecular complexes, or organelles have been labelled with the partner peptide, they can be targeted by the basic peptide that has entered the cell. (eurekalert.org)
Experiments with E. coli bacteria and eukaryotic cells revealed that the new peptide system is even suitable for transporting other peptides and proteins. (eurekalert.org)
Our investigations exemplify how the computational design of peptides and proteins, their subsequent synthesis and characterisation in the laboratory, as well as testing in living cells can intertwine when innovative solutions for biochemical or biomedical questions are being sought,' says Prof. Dr. Birte Höcker, head of the Protein Design Group at the University of Bayreuth and corresponding author of the new study. (eurekalert.org)
Dietary sterols have recently been shown to passively enter intestinal cells, and, subsequently, the vast majority are pumped back into the gut lumen by ATP-binding cassette (ABC) transporter proteins. (medscape.com)
Nikic I, Kang JH, Girona GE, Aramburu IV, Lemke EA (2015) Labeling proteins on live mammalian cells using click chemistry. (springer.com)
Autophagosomes engulf cytoplasmatic components and deliver these cargos, for example, long-lived proteins or damaged mitochondria, to lysosomes for degradation. (hindawi.com)

Check out relevant papers found by Labettor's AI that are relevant for performing Live / Dead assay mammalian cells - A549 using Cellstain-Double Staining Kit from Dojindo. (labettor.com)
In order to evaluate the premise that the ScanRDI™ assay is comparable to the growth-based membrane filtration assay for the purpose of detecting the presence of bioburden in cell culture samples, a master validation plan was drafted at Wyeth Biotech, Andover MA (USA), including elements of method evaluation, concurrent testing, method validation and crossover studies. (europeanpharmaceuticalreview.com)
In the application described herein, the ScanRDI™ assay is intended to detect a low-level bacterial contamination in a background of millions of Chinese Hamster Ovary (CHO) cells. (europeanpharmaceuticalreview.com)
Hydrodesulfurised kerosine was tested in the sister chromatid exchange assay using Chinese hamster ovary cells (API, 1988a). (europa.eu)
The assay was conducted with Aroclor-induced rat liver S-9 activation system. (europa.eu)
Under the conditions of the study, hydrodesulfurised kerosine is considered to be negative in the sister chromatid exchange assay with Chinese hamster ovary cells. (europa.eu)
Here, we present an adaptation of the bioluminescence resonance energy transfer (BRET)-based assay to specifically monitor activity of Gα12 in living cells. (nih.gov)
To facilitate the quantiﬁcation of cellular oxygenation Agilent Technologies has developed the MitoXpress Intra - Intracellular Oxygen Assay kit, based on a proprietary O 2 -sensitive cell-penetrating nanoparticle probe. (bmglabtech.com)
All tested compounds were modestly metabolically stable in human liver microsomes, exhibiting 30-60% metabolism over the course of the assay. (cdc.gov)

This is based on the fact that viruses within this subfamily, such as the Mammalian orthoreovirus, Aquareovirus, Coltivirus, Cypovirus, etc., have the presence of a turreted protein on the inner capsid. (wikipedia.org)
Just as the rest of the reoviruses are structured, the Mammalian orthoreovirus contains a segmented genome with a linear genomic arrangement, which is enclosed in a 70-80 nm double layered protein capsid, made up of an inner (T=2) and outer layer (T=13). (wikipedia.org)
Here, we have developed a new method which uses these cylindrical structures as platforms to detect protein interactions in cells. (hal.science)
Altogether, the results indicate that microtubules can be used as platforms to detect protein interactions in mammalian cells, which should provide a basis for investigating pathogenic protein interactions involved in human diseases. (hal.science)
The new peptide system makes it clear that de novo design is a promising research approach in the search for methods that enable drug or dye molecules to be introduced into mammalian cells in a targeted and gentle manner,' adds Dr Guto Rhys, postdoc at the Protein Design research group and one of the three first authors. (eurekalert.org)
Expression levels of PER2, a clock protein, were elevated in Sik3 -knockdown cells but down-regulated in Sik3 -overexpressing cells, which could be attributed to a phosphorylation-dependent decrease in PER2 protein stability. (elifesciences.org)
NADH:ubiquinone oxidoreductase, complex I, is one of the largest membrane protein assemblies known and has a central role in energy production by the mitochondrial respiratory chain, providing about 40 percent of the proton motive force required for the synthesis of adenosine triphosphate (ATP) that is the energy storage form of the cells. (mpg.de)
Ciprofibrate increased liver weights, lauric-acid-hydroxylase activity, and P4504A protein, but did not affect any other parameter. (cdc.gov)
Glutamine mediates protein turnover via cellular mTOR (mammalian target of rapamycin) signalling. (sigmaaldrich.com)
In particular, mammalian genomes contain 16 conserved genes encoding G protein α subunits with unique properties. (nih.gov)
The AstraZeneca vaccine uses an adenovirus as 'vector' to deliver a DNA sequence that human cells then use to create one specific (but harmless) SARS-CoV-2 protein. (moonofalabama.org)
The combination of genetic code expansion (GCE) and baculovirus-based protein expression in Spodoptera frugiperda cells is a powerful tool to express multiprotein complexes with site-specifically introduced noncanonical amino acids. (springer.com)
First is that every year, the hemagglutinin (the cell attachment protein that sits on the surface of the virus) mutates. (medscape.com)
Most PC gene mutations change a single protein building block (amino acid) in pyruvate carboxylase, which reduces the amount of this enzyme in cells or disrupts its ability to effectively convert pyruvate to oxaloacetate. (medlineplus.gov)

Although Legionella was the first pathogen demonstrated to multiply and persist in amoebae ( 18 ) , several other fastidious intracellular bacterial pathogens, including Chlamydia pneumoniae ( 19 ) , Mycobacterium avium ( 20 ) , Listeria monocytogenes ( 21 ) , and an Ehrlichia -like organism ( 22 ) , may infect free-living amoebae. (cdc.gov)
Receiving 80% of its blood supply from the intestine through the hepatic portal vein system, the liver is enriched with a large number of innate immune cells, which carry out the process of immune response by detecting pathogens entering the organism through the intestine. (bvsalud.org)
The interplay between the natural immune system and microbes coordinates the physiology of the whole organism, and in specific mammalian lineages, there is a dependency between the host and its associated microbes. (bvsalud.org)

1.2.3 In spite of this knowledge, the majority of in vitro studies culture and study cells at ambient oxygen - ignoring the oxygen gradient between the atmosphere and the medium and between the medium and the intracellular cell environment. (bmglabtech.com)

The second edition of Live Cell Imaging: A Laboratory Manual expands upon and extends the collection of established and evolving methods for studying dynamic changes in living cells and organisms presented in the well-known first edition. (cshlpress.com)
All living organisms encounter a wide variety of nutrient deprivations and environmental stresses. (duke.edu)
We are investigating their function using culture cells, MESH1 KO mice, and other model organisms. (duke.edu)

These ATG genes are highly conserved in mammalian cells, allowing to study their functions also in higher eukaryotes [ 1 - 4 ]. (hindawi.com)
Based on comparative genomics, 1473 bovine natural immunity genes were obtained by collecting the latest reports of human natural immunity genes and updated bovine genomic data for comparison, and a bovine natural immunity gene database was initially constructed to screen and match calf liver natural immunity differential genes mainly affected by the phylum Mimosoidea and the phylum Thick-walled Bacteria, and 16 differentially expressed natural immunity genes were obtained. (bvsalud.org)

Communication between dermal papilla cells and the overlying epithelium is essential for differentiation of the hair follicle lineages. (biologists.com)

Cytosolic lipids participate in the growth, development, and overall health of mammalian oocytes including many roles in cellular homeostasis. (rsc.org)
Ultimately, the goal is to develop new imaging methods that uncover what roles weak and transient interactions play in regulating gene expression in single living cells. (mit.edu)

Research in the past 20 years revealed that non-glandular organs such as adipose tissue, liver and skeletal muscle can secrete hormones that regulate whole-body metabolism. (upenn.edu)
Whether you're starting from primary cells, tissue samples, or pluripotent stem cells, explore our complete portfolio of products to support your neural culture workflow. (stemcell.com)
Recent advances in live imaging and genetics of mammalian division, movement and cell differentiation leading to development which integrate observations of biochemical tissue formation [14 ]. (lu.se)
of the most critical events which lead to tissue patterning involves mechanisms going beyond single cells. (lu.se)
There are three subunit isozymes of PFK in mammalian cells: muscle (M), liver (L), and platelet (P or C). Active PFK exists as a tetramer, and the composition of subunits differs according to the tissue type. (medscape.com)
The experimental part involves the use of diverse AAV libraries with peptide insert modifications, designed to enhance AAV tropism and enable specific targeting of neuronal and non-neuronal populations in the central nervous system (CNS) and other tissue and cell types. (lu.se)
Tissue samples will be collected from mammalian species, ranging from rodents to non-human primates (NHPs), and subjected to various sequencing methods, including bulk sequencing of tissue samples, 10X single nuclei sequencing, and Parse Bio single nuclei sequencing. (lu.se)
Statistical analysis, visualization, and comparative studies will be performed to evaluate the performance of different AAV variants across various cell types and tissue types. (lu.se)
Special attention will be given to avoiding non-specific tissue infection, particularly in the liver, as it is a common site for unwanted AAV vector delivery. (lu.se)
The liver is not only a digestive gland, but also an immune tissue that receives a double infusion from the hepatic artery and the hepatic portal vein. (bvsalud.org)
It is described as a housekeeping enzyme, being responsible for cell-to-cell signaling, tissue homeostasis, and cytoprotection. (medscape.com)

The detection and enumeration of microorganisms is achieved by the system through a sequential series of computerised algorithms, which discriminate between fluorescent signal generated by bacterial cells and nonspecific fluorescence generated by debris or other material on the filter membrane. (europeanpharmaceuticalreview.com)
Several years ago we finished the development of a 48-channel instrument, which primarily was designed for measurements of the activity of microorganisms, mammalian cells and biopsies in the pharmaceutical industry and in clinical laboratories. (lu.se)

Results of this study demonstrate that CARS is able to identify lipids in live mammalian oocytes, and there exists quantifiable and consistent differences in percent lipid composition across ooctyes of different species, developmental stages, and in relation to body composition. (rsc.org)

We are broadly interested in understanding how cells answer these questions and we address them by using multi-disciplinary approaches in yeast and human cell culture, such as ribosome profiling and computational analysis, biochemical techniques, and single-molecule fluorescence imaging. (nih.gov)
New analytical options include live high-throughput/high-content screening in mammalian cells and computational analysis of live cell data. (cshlpress.com)

Such a method of live-cell lipid quantification has (i) experimental power in basic cell biology, (ii) practical utility for identifying developmental predictive biomarkers while advancing biology-based oocyte/embryo selection, and (iii) ability to yield rationally supporting technology for decision-making in rodents, domestic species, and human assisted reproduction and/or fertility preservation. (rsc.org)
As seen in the name, the Mammalian Ortheoreovirus infects numerous mammalian species and vertebrates which serve as natural hosts. (wikipedia.org)
There are four serotypes, or distinct variations within the viral species, of Mammalian orthoreovirus. (wikipedia.org)
A number of standard tests, with and without metabolic activation by inclusion of rat liver S9, were conducted on straight-run kerosines, deodorised kerosine and Jet A. A rather wide variety of tester strains, both of prokaryotic species ( Salmonella typhimurium ) and eukaryotic species ( Saccharomyces cerevisiae ), were used, and also various solvents were applied to dissolve the test product. (europa.eu)
Rowbotham described the ability of L. pneumophila to multiply intracellularly within protozoa ( 18 ) and suggested that free-living amoebae could be a reservoir for Legionella species ( 24 ) . (cdc.gov)
Growth of free-living amoebae at high temperatures (44°C to 53°C) was observed more frequently for strains isolated from hot-water tanks (mainly Hartmanella vermiformis ) than for those isolated from moist sanitary areas (mainly Acanthamoeba , Naegleria, and Valkhampfia species) ( 26 ) . (cdc.gov)
In contrast to individuals with classic Tarui disease, who express only the L4 type isozyme in red blood cells, individuals with late-onset Tarui disease showed the presence of a few hybrid isozymes of M+L with the predominant L4 species, suggesting a "leaky" mutation of the gene coding the M subunit. (medscape.com)
The objective of this Master thesis project is to develop a comprehensive bioinformatic pipeline that enables the identification of DNA barcodes associated with AAV vectors, thereby facilitating the evaluation of AAV variant efficiency and tropism in mammalian species. (lu.se)

However, existing methods are lengthy, mostly due to the step of scraping cells from cell culture vessels, which restricts metabolomics in broader application such as lower cell numbers and high-throughput studies. (frontiersin.org)

His main research areas are developmental biology, cell morphogenesis, adult stem cells and cardiac function analysis. (amrita.edu)

Second, we have identified the mammalian stringent response pathway which is highly similar to bacterial stringent response, but with some very interesting twists and novel mechanisms. (duke.edu)
we have found the Hippo pathway effectors TAZ and YAP are responsible for the cell density-dependent ferroptosis (4, 6, 7). (duke.edu)
Therefore, we termed this pathway as "mammalian stringent response" (12). (duke.edu)
d) Identify the liver GDF15 receptor and elucidate its signaling pathway in the liver. (upenn.edu)
and RIG-I-like receptor signaling pathway, cell cycle, and actin cytoskeleton regulation. (bvsalud.org)

calcein-AM, is able to stain viable cells (green), while propidium iodide, a nucleus-staining dye that is unable to penetrate the cell membranes of viable cells, can stain only dead cells (red). (labettor.com)
It is used to produce hormones and cell membranes and is transported in the blood plasma of all mammals. (absoluteastronomy.com)
It is an essential structural component of mammalian cell membranes. (absoluteastronomy.com)

Here, we present a simplified metabolite extraction (SiMeEx) method, to efficiently and quickly extract metabolites from adherent mammalian cells. (frontiersin.org)
We demonstrate that SiMeEx achieves the same metabolite recovery as using a standard method containing a scraping step, in various immortalized and primary cells. (frontiersin.org)
In this current study, we present a new and shorter method, termed as simplified metabolite extraction (SiMeEx), to extract metabolites from adherent mammalian cells. (frontiersin.org)
Any mammalian metabolite produced during a metabolic reaction in humans ( Homo sapiens ). (ebi.ac.uk)
Any metabolite produced by all living cells. (ebi.ac.uk)
Any mammalian metabolite produced during a metabolic reaction in a mouse ( Mus musculus ). (ebi.ac.uk)

We demonstrate the ability of two different Riboglow probes to track mRNA and small non-coding U RNA in live mammalian cells. (colorado.edu)
mRNA half-lives have been determined, for example, by blockage of transcription followed by transcriptional profiling 5 . (nature.com)

This means that there are four separate strains of Mammalian orthoreovirus: Type 1 Lang, Type 2 Jones, Type 3 Dearing, Type 4 Ndelle. (wikipedia.org)
In a study by the American Petroleum Institute (1977) (Klimisch score = 1), strains of S. typhimurium (TA-1535, TA-1537, TA-1538, TA-98 and TA-100) and S. cerevisiae (D4) were exposed to kerosine in DMSO with or without metabolic activation by Aroclor 1254-induced rat liver microsomes at dose concentrations from 0.001 to 5.0 μL/plate. (europa.eu)
In laboratory experiments, the original U.S. survivor's strain did not cause damage to cells as rapidly as other strains, suggesting that it is less virulent than strains recovered from other fatal infections 13 . (cdc.gov)

proteolysis_Utilizing flow cytometry to monitor autophagy in living mammalian cells. (proteolysis.jp)
The labeled cells can be visualized using fluorescent imaging, quantified using flow cytometry, and isolated using fluorescence-activated cell sorting (FACS).The ALDEFLUOR™ fluorescent reagent system offers a novel approach to the non-immunological identification, evaluation and isolation of neural stem and progenitor cells. (stemcell.com)

Serum surveys conducted in the 1950s found neutralizing antibodies to mammalian orthoreovirus in humans, monkeys, rabbits and guinea pigs. (wikipedia.org)
Primary antibodies are available for the characterization of neural stem cells, neural progenitor cells and their progeny. (stemcell.com)

Although awareness of the importance of pH is fostered early in the training of researchers, there are no consensus guidelines for best practice in managing pH in cell cultures, and reporting standards relating to pH are typically inadequate. (ox.ac.uk)
In a study by the American Petroleum Institute (API, 1984b), cultures of mouse lymphoma cells were exposed to hydrodesulfurised kerosine with or without metabolic activation by Aroclor 1254-induced rat liver S9 fraction. (europa.eu)
The MitoXpress Intra nanoparticle probe is taken up by cells during an overnight loading period and responds in real-time to any changes in intracellular oxygen concentration in both 2D cultures as well as a wide range of 3D systems, including Matrigel, RAFT™, microtissues, Alvetex ® , Mimetix ®, and other scaffolds. (bmglabtech.com)

Previously, we have identified the profound cystine addiction of renal cell carcinoma (1), breast cancer cells (2, 3), and ovarian cancer cells (4). (duke.edu)
However, the role of SHMT in patients with renal cell carcinoma (RCC) has not been fully elucidated. (bvsalud.org)

For cell viability assays, monolayers of A549 cells were cultured in 24-well plates to a density of 1 × 105 cells per well. (labettor.com)
Owing to the time required for results using traditional bioburden monitoring assays, the cell culture process must continue at risk until the results are available. (europeanpharmaceuticalreview.com)
In skin reconstitution assays, hair follicles failed to form when dermis was depleted of both GFP + CD133 + and GFP - CD133 + cells. (biologists.com)
The ACU is a microprocessor-controlled unit that can regulate CO 2 and O 2 within the reader to reproduce the optimal physiological as well as hypoxic conditions needed for live cell-based assays . (bmglabtech.com)

Non-alcoholic fatty liver disease (NAFLD), one of the most significant forms of chronic liver disease worldwide, is characterized by hepatic steatosis. (frontiersin.org)

The Mammalian orthoreovirus double stranded RNA genome contains 10 segments divided into three size classes (small, medium, and large) based upon their characteristic mobility during gel electrophoresis. (wikipedia.org)

Enzyme replacement therapy (ERT) with glucocerebrosidase purified from human placenta was FDA approved in 1991, followed by approval in 1994 of a recombinant form of the enzyme produced in cultured Chinese hamster ovary (CHO) cells, marketed as imiglucerase (Cerezyme). (medscape.com)

It is believed that Mammalian orthoreovirus causes subclinical infection in humans more so than in other mammals. (wikipedia.org)

The Mammalian orthoreovirus is well researched and understood on the biochemical and structural levels, additionally their pathogenesis in mice serves as a model system for studying the pathogenesis of the reoviruses in general. (wikipedia.org)
This partner peptide is such that it can be placed in very different locations inside the cell using established biochemical methods. (eurekalert.org)

Reduces spleen and liver volume and increases hemoglobin and platelet counts. (medscape.com)

These ABC transporters preferentially pump plant sterols out of intestinal cells into the gut lumen and out of liver cells into the bile ducts, thereby decreasing sterol absorption. (medscape.com)
The intestine and liver communicate extensively through the bile ducts, portal vein and the somatic circulation, a bidirectional communication known as the intestinal-liver axis. (bvsalud.org)

A549 human alveolar epithelial cells were cultured in Dulbecco modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum, 100 mg/liter of penicillin, and 100 mg/liter streptomycin at 37°C in the presence of 10% CO2. (labettor.com)
Meaning, that a human infected with Mammalian orthoreovirus, regardless of the serotype, is nearly or completely asymptomatic, and therefore, don't exhibit signs or symptoms of the virus. (wikipedia.org)
Synthesis:Estriol is only produced in significant amounts during pregnancy as it is made by the placenta from 16-Hydroxydehydroepiandrosterone sulfate , an androgen steroid made in the fetal liver and adrenal glands.The human. (absoluteastronomy.com)
We used primary acute myeloid leukemia (AML) samples and human AML cell lines to investigate the regulatory mechanisms of autophagy and its role in AML differentiation. (hindawi.com)
So, there was a human infection back in 2002 in Virginia, and then another human case that was detected in New York in a person that visited these live poultry markets. (cdc.gov)
Mammalian cell lines were subjected to extensive safety testing to establish a cell line that is human pathogens free, while maintaining sufficient vaccine yield. (cdc.gov)
This form of recombinant enzyme is produced in cultured human cells and is marketed as velaglucerase alfa (VPRIV). (medscape.com)

In the speciﬁc example shown here (Fig. 1) for cells cultured under ambient oxygen, the intracellular [O 2 ] measured using MitoXpress Intra was found to be ~14% for metabolically inactive cells and ~7% for metabolically active cell types. (bmglabtech.com)
However, when the environmental O 2 concentration is reduced to ~6% using an ACU, the intracellular [O 2 ] falls to ~4.5% for metabolically less active cells and is close to anoxia for metabolically active cells. (bmglabtech.com)

Microtubules are μm-long cylinders of about 25 nm in diameter which are present in the cytoplasm of eukaryotic cells. (hal.science)

During early-stage cell apoptosis, PS is translocated from the inner to the outer leaflet of the plasma membrane, exposing it to the external environment. (stemcell.com)
Annexin V, a characteristic marker for early cell apoptosis, detects the translocation of PS to the external environment. (stemcell.com)
Therefore, as cells progress through apoptosis and towards necrosis, the cell membrane is compromised and consequently, viability dyes pass into the cell. (stemcell.com)
Thus, cells undergoing early apoptosis stain positive for Annexin V and negative for viability dyes, while apoptotic death or necrosis is characterized by positive staining for both Annexin V and the viability dye. (stemcell.com)

A novel peptide developed at the Universities of Bayreuth and Bristol is eminently suited for the targeted transport of molecules, for example of active substances and dyes, into the cells of mammals. (eurekalert.org)

As the real oxygen concentration experienced by cells in culture is a function of environmental O 2 concentration, cell metabolism, and seeding density, MitoXpress Intra provides the ideal tool to intelligently modulate these parameters to achieve a desired and speciﬁc intra-cellular oxygen concentration. (bmglabtech.com)

Data revealed that the microbeads prepared with double-layered structure possess optimal cells proliferation rate and parathyroid hormone (PTH) secretion capability, which shows no significant difference compared with a control group. (ncl.edu.tw)
Dr. Rajaguru Aradhya and Jagla, K., "Insulin-dependent Non-canonical Activation of Notch in Drosophila: A Story of Notch-Induced Muscle Stem Cell Proliferation", in Notch Signaling in Embryology and Cancer: Molecular Biology of Notch Signaling, vol. 1227, J. Reichrath and Reichrath, S., Eds. (amrita.edu)

Hahn AT, Jones JT, Meyer T. Quantitative analysis of cell cycle phase durations and PC12 differentiation using fluorescent biosensors. (harvard.edu)

It is applied once to the wound to aid healing and regeneration of skin cells. (helsinki.fi)
and stem cell regeneration. (lu.se)

Fluka, Buchs, Switzerland) was used according to the manufacturer's instructions to measure cell viability postinfection. (labettor.com)

Autophagy is an intracellular degradation system that ensures a dynamic recycling of a variety of building blocks required for self-renewal, homeostasis, and cell survival under stress. (hindawi.com)
Basal macroautophagy (thereafter referred to as autophagy), a catabolic recycling system in cells, is key to maintaining cellular homeostasis and survival. (hindawi.com)
Furthermore, activation of autophagy allows to extend cell survival when exposed to different types of stressors such as starvation or cytotoxic drugs. (hindawi.com)
While the importance of autophagy for cell homeostasis and survival has long been appreciated, its role in tumorigenesis and cancer progression is still developing [ 10 , 11 ]. (hindawi.com)

The movies on the accompanying website www.cshprotocols.org/livecellimaging display the power of recent advances in technical imaging of living cells. (cshlpress.com)
They include advances in atomic force microscopy, structured illumination microscopy and other 3-D approaches, as well as imaging in single cells in animals and in plants. (cshlpress.com)
the second provides detailed protocols for imaging live systems. (cshlpress.com)
In living cells, collective behaviors emerge from weak and transient biological interactions that are difficult to detect in conventional cell imaging methods. (mit.edu)

A recombinant-derived analog of beta-glucocerebrosidase produced in mammalian cell culture and chemically modified by mannose termination of glycosylated amino acids. (medscape.com)

Fluorescent labelling of rRNA has been achieved in live HeLa cells via an inverse electron demand Diels-Alder reaction with a tetrazine conjugated to an Oregon Green fluorophore. (rsc.org)

We are using functional genomic approaches to investigate the nutrient signaling and stress adaptations of cancer cells when exposed to various nutrient deprivations and microenvironmental stress conditions. (duke.edu)

This enzyme is active in mitochondria, which are the energy-producing centers within cells. (medlineplus.gov)

Now, with that said, if you look at the number of deaths that have been prevented by the influenza vaccine between the 2005-2006 season and the 2013-2014 season, we have saved an estimated 40,000 lives with the influenza vaccine. (medscape.com)
Cite this: A Bad Flu Year: Lives Are Saved Even With a 'Less Effective' Vaccine - Medscape - Jan 30, 2018. (medscape.com)

Optimal cell performance usually requires supplementation of the medium with L-glutamine prior to use. (sigmaaldrich.com)

Omitting cell scraping does not compromise the performance of non-targeted and targeted GC-MS analysis, but enables metabolome analysis of cell culture on smaller well sizes down to 96-well plates. (frontiersin.org)
For adherent mammalian cells in culture the normal procedure involves their detachment from the surface. (frontiersin.org)
One type of cell culture manufacturing process, called a batch refeed process, can be used to demonstrate two main aspects of the cell culture operation which will further exemplify the importance of timely results. (europeanpharmaceuticalreview.com)
Here, we use real-time measurements of medium pH and intracellular pH under live-cell culture conditions to describe the effects of various buffering regimes, including physiological CO2/HCO3− and non-volatile buffers (e.g. (ox.ac.uk)
L-glutamine is an essential amino acid that is a crucial component of culture media, and serves as a major energy source for cells in culture. (sigmaaldrich.com)
Qualified for use in cell culture applications, especially serum-free cell culture. (sigmaaldrich.com)

The aggregation of the amyloidogenic polypeptide IAPP (Islet Amyloid Polypeptide, amylin) is believed to play a direct role in the death of pancreatic β-islet cells in type II diabetes. (openbiochemistryjournal.com)
To this end, light can either help drugs reach the desired cell type or cause drugs to be released at a particular site. (helsinki.fi)
When applied to a mouse model of pediatric mitochondrial cardiomyopathy, we uncovered profound cell type-specific modifications of the cardiac transcriptional landscape at single-nucleus resolution, including changes of subtype composition, maturation states, and functional remodeling of each cell type. (upenn.edu)
d) Single-cell multiomics to understand cell-type specific metabolic changes in heart disease. (upenn.edu)
Трансплантація печінки Liver transplantation is the 2nd most common type of solid organ transplantation. (msdmanuals.com)

This was further confirmed by PER2 accumulation in the Sik3 -/- fibroblasts and liver. (elifesciences.org)
By profiling the transcriptome of ~24,000 nuclei, we identified major and rare cardiac cell types and revealed significant heterogeneity of cardiomyocytes, fibroblasts, and endothelial cells in postnatal developing hearts. (upenn.edu)

However, all these efforts share a common cell-scraping step, which limits further optimization of the extraction efficiency. (frontiersin.org)

Single cell experiments [ 11 ] could provide iterative validation of models with experimental results. (lu.se)

Prior to the PhD he also worked in the laboratory of Dr. Maneesha Inamdar, JNCASR, India, where he worked on the characterization of pericardial cells in Drosophila Melanogaster. (amrita.edu)

Thus, free-living amoebae could readily act as Trojan horses for bacterial endosymbionts ( 33 , 34 ). (cdc.gov)

The liver and kidneys express predominately the L isoform. (medscape.com)
In the kidneys and liver, it is the first step in a process called gluconeogenesis. (medlineplus.gov)

In addition, different cell types exhibit preference for specific metabolic pathways (fatty acid oxidation/FAO, glycolysis or oxidative phosphorylation/OxPhos). (upenn.edu)
The Eis inhibitors generally did not exhibit cytotoxicity against mammalian cells. (cdc.gov)

In recent years, biomedicine and pharmacology have developed a large number of active substances that are capable of triggering, enhancing, or inhibiting processes in mammalian cells. (eurekalert.org)
RNAs directly regulate a vast array of critical cellular processes, emphasizing the need for robust approaches to fluorescently tag and track RNAs in living cells. (colorado.edu)

Microscopic images of the stained cells (alive and dead) were obtained using an inverted fluorescence microscope (Nikon Eclipse Ti) and analyzed with the NIS Elements Br software package. (labettor.com)

In other words can I take a 10 cm dish containing EGFP expressing cells and visualize them unsing fluorescent microscopy? (bio.net)
Nikic I, Plass T, Schraidt O, Szymanski J, Briggs JA, Schultz C, Lemke EA (2014) Minimal tags for rapid dual-color live-cell labeling and super-resolution microscopy. (springer.com)

1 Wellcome Trust Centre for Stem Cell Research, Tennis Court Road, Cambridge CB2 1QR, UK. (biologists.com)
Трансплантація гемопоетичних стовбурових клітин Hematopoietic stem cell (HSC) transplantation is a rapidly evolving technique that offers a potential cure for hematologic cancers ( leukemias, lymphomas, myeloma) and other hematologic disorders. (msdmanuals.com)
Living donors are often used for kidney and hematopoietic stem cell transplants, less frequently for segmental liver transplants, and rarely for pancreas and lung transplants. (msdmanuals.com)

The mammalian gut is considered an important microbial ecosystem. (bvsalud.org)

Anatomy20

Organisms8

Chemicals and Drugs22

Analytical, Diagnostic and Therapeutic Techniques and Equipment12

Phenomena and Processes36

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Named Groups1

Health Care2

Tissues10

Studies in mammalian2

Proteins8

Assay9

Protein14

Organism3

Vitro1

Organisms3

Genes2

Lineages1

Roles2

Tissue11

Microorganisms2

Lipids1

Computational analysis2

Species8

High-throughput1

Developmental1

Pathway5

Membranes3

Metabolite6

MRNA2

Strains3

Flow cytometry2

Antibodies2

Cultures3

Renal cell carc2

Assays4

Hepatic1

Genome1

Chinese Hamst1

Humans1

Biochemical2

Platelet1

Bile ducts2

Human7

Metabolically2

Eukaryotic cells1

Apoptosis4

Mammals1

Metabolism1

Proliferation2

Differentiation1

Regeneration2

Viability1

Autophagy4

Imaging4

Recombinant1

HeLa1

Genomic1

Enzyme1

Vaccine2

Optimal1

Culture6

Type5

Fibroblasts2

Efficiency1

Experiments1

Characterization1

Bacterial1

Kidneys2

Exhibit2

Processes2

Fluorescence1

Microscopy2

Stem cell3

Microbial1