• In this study, the effects of ion-pairing agents and other ingredients of mobile phase on the separation of nor adrenaline (NA), adrenaline (A) and dopamine (DA) were studied in reversed-phase high performance liquid chromatography with electrochemical detection. (medicaljournal-ias.org)
  • High-performance liquid chromatography is an appropriate technology for this purpose, and the stationary phase is the kernel to the separation efficiency . (bvsalud.org)
  • Find out here how Bürkert can support you with energy-efficient valves and demand-oriented automation concepts for safe and smooth production, for example in liquid chromatography or high-performance liquid chromatography (HPLC). (burkert.com)
  • Selective separation of the analytes is achieved using high-performance liquid chromatography with a gradient elution program. (cdc.gov)
  • The ether bond internal to N- acetylmuramic acid was cleaved with 3% ammonia, and the resulting lactoyl peptides were separated by reversed-phase, high-performance liquid chromatography for sequencing by tandem mass spectrometry (Qstar Pulsar I). (cdc.gov)
  • The major components in MWFs were separated by high performance liquid chromatography (HPLC) and detected by both ultraviolet (UV) absorption and evaporative light scattering detection (ELSD). (cdc.gov)
  • Instrumentation and simple troubleshooting for high-performance liquid chromatography with universal and selective detection. (lu.se)
  • HSA was found to be a suitable ion-pairing agent for liquid chromatographic separation of CAs in either phosphate or ammonium acetate based mobile phases and the applicability of the method was demonstrated by analysis of CAs in different rat tissues after alumina extraction. (medicaljournal-ias.org)
  • Fast reversed-phase liquid chromatographic separation of proteins by flow-through poly(styrene-co-divinylbenzene) microspheres. (bvsalud.org)
  • Bioanalytical HPLC includes advanced theoretical treatment of liquid chromatographic separation modes and the underlying distribution and adsorption equilibria including reversed-phase chromatography, normal-phase chromatography, ion chromatography, ion-pair chromatography, hydrophilic interaction liquid chromatography (HILIC), size exclusion chromatography and supercritical fluid chromatography. (lu.se)
  • Lectures and exercises (7.5 credits): In-depth theoretical treatment of chromatographic separation and underlying distribution and adsorption equilibria. (lu.se)
  • Liquid-liquid phase separation (LLPS) is well defined in the Biomolecular condensate page. (wikipedia.org)
  • Here, we found that the C-terminus of IRS-1 undergoes liquid-liquid phase separation (LLPS). (nature.com)
  • Importantly, metabolic disease-derived G972R mutation results in a reduced ability of LLPS, potentially implicating the involvement of aberrant IRS-1 phase separation in various metabolic disorders. (nature.com)
  • Recently, tau has been shown to undergo liquid liquid phase separation (LLPS) both in vivo and in vitro . (biorxiv.org)
  • Swi6 - an HP1 orthologue in Schizosaccharomyces pombe (S. pombe) - can both undergo liquid-liquid phase separation (LLPS) and compact nucleosomes in vitro [Sanulli, 2018]. (emerging-researchers.org)
  • We hypothesize that swi6-sm1, an allele of Swi6, may be such a separation-of-function allele, as the mutations that comprise the swi6-sm1 allele are in the N-terminal extension, a region of Swi6 implicated in its LLPS activity [Larson, 2017]. (emerging-researchers.org)
  • Recent evidence has shown that the processes of liquid-liquid phase separation (LLPS) or liquid-liquid phase transitions (LLPTs) are a crucial and prevalent phenomenon that underlies the biogenesis of numerous membrane-less organelles (MLOs) and biomolecular condensates within the cells. (usf.edu)
  • Additionally, the finding of mis-regulated proteins, which have long been linked to aggregates in neuropathology, are also known to induce LLPS/LLPTs, prompting a lot of interest in understanding the connection between aberrant phase separation and disorder conditions. (usf.edu)
  • Here, we report that liquid-liquid phase separation (LLPS) could drive the formation of robust, stable, and sustainable plastics using small molecules. (abo.fi)
  • Proceeds from this financing are anticipated to be used to enhance the Protein Liquid-Liquid Phase Separation (LLPS) technology platform and advance multiple pipelines through discovery and clinical development. (voiceofasean.com)
  • With the world-leading Protein Liquid-Liquid Phase Separation (LLPS) technology platform, ETERN has built multiple pipelines and advanced one to the clinical stage to provide new solutions to cancer patients. (voiceofasean.com)
  • The second one is an extraction-based separation of a miscible multi-component mixture through a supported ionic-liquid membrane (SILM). (aiche.org)
  • The miscibility between the impregnated ionic liquid and one of the mixture components, but not the other, is the driving force for this miscible multi-component separation. (aiche.org)
  • Since the continuous emergence of novel fentanyl analogues has created a need for improved methods able to distinguish between fentanyl analogues and their isomers, in this study, the utility of commercially available ionic liquid columns is demonstrated for GC-FID and GC-MS separation and unambiguous identification of fentanyl, 22 fentanyl analogues, and U-47700. (ojp.gov)
  • 24 compounds were separated 36% faster with an elution window increase of 33% on the ionic liquid phase compared to the 5% diphenyl/95% dimethylpolysiloxane phase. (ojp.gov)
  • The selectivity of 11 isomeric analogue sets (out of 12) increased by 2 to 25% over the 5% diphenyl/95% dimethylpolysiloxane phase showing an ionic liquid column is more favorable for separating fentanyl analogues. (ojp.gov)
  • The ionic liquid column provides better signal to noise, and a faster and more selective option for forensic laboratories looking to identify confiscated illicit drug samples. (ojp.gov)
  • Optimal Aqueous Biphasic Systems Design for the Recovery of Ionic Liquids. (acs.org)
  • Formation of Aqueous Biphasic Systems with an Ionic Liquid Induced by Metallic Salts: Nanoscopic Views from Molecular Dynamics Simulations. (acs.org)
  • Effect of Salt Type and Alkyl Chain Length on the Binodal Curve of an Aqueous Two-Phase System Composed of Imidazolium Ionic Liquids. (acs.org)
  • These novel classes of liquid materials include ionic liquids, CO 2 -triggered switchable solvents (i.e. (frontiersin.org)
  • CO 2 -binding organic liquids, reversible ionic liquids), and nanoparticle organic hybrid materials. (frontiersin.org)
  • In particular, the recent investigation of novel anhydrous liquid materials, such as ionic liquids (ILs), CO 2 -triggered switchable absorbents [i.e. (frontiersin.org)
  • Vapourisation of ionic liquids Physical Chemistry Chemical Physics. (nottingham.ac.uk)
  • In the case of the perhalogenated compounds (CFC-113, CFC-114, and their isomers), the solubility behavior between isomers in the ionic liquid is practically identical with large immiscibility gaps. (acs.org)
  • This suggests that the (present) ionic liquid cannot be used for these isomer separations. (acs.org)
  • However, the monohydrogen substituted halocarbons (HCFC-123, HCFC-124, and their isomers) begin to show some difference (liquid−liquid immiscibility gap) in the ionic liquid. (acs.org)
  • The isomer effect on the solubility in the ionic liquid becomes significant for the dihydrogen-substituted halocarbons (HFC-134 and HFC-134a), and these isomers can be separated using [emim][Tf 2 N] as an entrainer in an extractive distillation. (acs.org)
  • This observation is consistent with our earlier findings for various HFCs in ionic liquids. (acs.org)
  • Ionic Liquid Selection for the Separation of Refrigerant Mixtures Using Extractive Distillation. (acs.org)
  • First Measurements of the Sorption of Difluoromethane and Pentafluoroethane in Encapsulated Ionic Liquids. (acs.org)
  • What Data Are Most Valuable to Screen Ionic Liquid Entrainers for Hydrofluorocarbon Refrigerant Reuse and Recycling? (acs.org)
  • In addition to maintaining specific organelle structures, phase separation enables hub proteins to assemble signalosomes which promote the speed of signaling outputs 11 , 12 . (nature.com)
  • Since these intrinsically disordered regions, which are conformationally dynamic and do not adopt stable secondary or tertiary structures, are often essential for mediating the phase transition of proteins 9 , it is therefore of interest to consider if the C-terminus of IRS-1 is involved in phase separation and to further delineate such implications upon insulin/IGF signaling. (nature.com)
  • Many proteins found in pathological inclusions are known to undergo liquid-liquid phase separation, a reversible process of molecular self-assembly. (nih.gov)
  • Here, we review similarities and differences among four main proteins, α-synuclein, FUS, tau, and TDP-43, which are found aggregated in different diseases and were independently shown to phase separate. (nih.gov)
  • With the explosive growth of the bioscience and biopharmaceuticals , the demand for high efficient analysis and separation of proteins is urgent. (bvsalud.org)
  • Owing to the hydrophobic nature , poly( styrene -co-divinylbenzene) microspheres were suitable as the reversed-phase stationary phase for separation of proteins . (bvsalud.org)
  • For the high permeability of the poly( styrene -co-divinylbenzene) microspheres packed column, fast separation of the studied six proteins in â ¼2 min was achieved. (bvsalud.org)
  • A subunit of the enzymatic complex P-TEFb can induce compartmentalization of proteins into liquid-like droplets in cells. (natureasia.com)
  • Expected announcement of the position is spring 2023, and the project will focus on liquid-liquid phase separation mediated by phosphorylated intrinsically disordered proteins utilizing computer simulations in combination with SAXS. (lu.se)
  • Liquid-liquid phase separation of nucleocapsid proteins during SARS-CoV-2 and HIV-1 replication. (cdc.gov)
  • Applications are invited for two postdoctoral positions to develop computational approaches to study (1) the phenomena of liquid-liquid phase separation of heterogenous mixtures of nucleic acids and proteins occurring inside the Cell nucleus and (2) the organization of chromatin with subnucleosome resolution inside megabase scale domains. (lu.se)
  • A comprehensive two-dimensional liquid chromatography system was constructed on the combination of size exclusion chromatography (SEC) and reverse phase liquid chromatography (RPLC). (edu.hk)
  • The results show that comprehensive two-dimensional liquid chromatography system is of great importance and high value in the separation of complex TCM. (edu.hk)
  • To purify liquids (downstream processing) out of a variety of components, a liquid needs to go through a chromatography process. (burkert.com)
  • The high separation power and short analysis time obtained demonstrate for the first time that supercritical fluid chromatography is a fast and reliable technique for the analysis of lignin-derived phenols in complex environmental samples. (lu.se)
  • UHPSFC , ultra- pacted by other parameters, such as the addition of the mod- high performance supercritical fluid chromatography ifier and the change of the mobile phase density [20]. (lu.se)
  • This course covers modern liquid chromatography (HPLC and UHPLC) that are especially used for bioanalytical applications. (lu.se)
  • For accurate quantitative or qualitative analysis, liquid chromatography is today combined with different mass spectrometric techniques. (lu.se)
  • For a deeper insight into mass spectrometric detection the LifeScience course "Mass spectrometry for small molecules" is recommended.course covers modern liquid chromatography (HPLC) especially for bioanalytical applications. (lu.se)
  • The aim of the course is to enable students to acquire in depth theoretical and practical knowledge of common modern separation techniques, such as chromatography, mass spectrometry and capillary electrophoresis. (lu.se)
  • Mass spectrometric detection for quantitative and qualitative analysis connected to both gas and liquid chromatography. (lu.se)
  • Mechanisms of chiral discrimination by these and other types of chiral HPLC phases are discussed in terms of the interactions between functionalitles in the stationary phase and solute enantiomers. (warwick.ac.uk)
  • A method was conducted that allowed the analysis of arsenic animal feed additives by ion pairing-reversed phase HPLC (IP-RP-HPLC) with a UV detection using an octadecylsilylsilica column. (utm.my)
  • Optimisation of HPLC systems with different combinations of mobile and stationary phases. (lu.se)
  • Sample preparation of biological samples and optimisation strategies for reversed-phase liquid chromatographic applications including selection of column and mobile phase compositions. (lu.se)
  • Currently, I work on topics such as protein folding in cellular environments, protein aggregation, and phase separation of biomolecular condensates. (lu.se)
  • Phase separation of liquids, as illustrated by everyday oil and water mixtures, provides the basis for creating diverse soft materials, from lipid vesicles to emulsions. (technion.ac.il)
  • In phase separating mixtures of an active isotropic fluid and a passive fluid, bulk active flows produce interfacial waves whose amplitude is controlled by activity. (technion.ac.il)
  • The three-phase centrifuge separates solid-liquid three-phase mixtures by rotating at high speed and producing a centrifugal force thousands of times larger than the gravity force. (zkcentrifuge.com)
  • Although both liquid mixtures and pure fluids belong to the same universality class, both the mass transport mechanisms and their thermophysical properties are significantly different. (epj.org)
  • In supercritical pure fluids the transport of heat and mass are strongly coupled by the enthalpy of condensation, whereas in liquid mixtures mass transport processes are purely diffusive. (epj.org)
  • The viscosity is also much smaller in pure fluids than in liquid mixtures. (epj.org)
  • The Influence Of Air On The Magneto-Vibratory Separation Of Binary Granular Mixtures Granular Matter. (nottingham.ac.uk)
  • Liquid and gas phase chromatographic methods were used to characterize the composition of these complex mixtures. (cdc.gov)
  • Although membrane-based separation technologies represent an energy-efficient alternative to traditional methodologies (e.g., fractional distillation), such processes are inherently limited to single selectivity. (aiche.org)
  • The other four carbamate phases all showed resolving ability, each with its own specific pattern of solute selectivity. (warwick.ac.uk)
  • A proposed mechanism for this unique ability combines the shape selectivity of polymeric phases, previously recognized for planar polycyclic aromatic hydro¬ carbons, and the different 'molecular footprints' of the isomers. (canada.ca)
  • We have assembled the two membranes in a single 3D-printed integrated membrane device and demonstrated the successful separation of benzene/heptane/water, a three-component and two-phase liquid mixture. (aiche.org)
  • They currently aim to develop robust adsorbents and membranes with improved performances for water purification and gas separation, and study the fundamental structure-property relationships in these systems. (uwm.edu)
  • IGF-1 stimulation enhanced IRS-1 phase separation which then recruited downstream effectors to form insulin/IGF signalosomes. (nature.com)
  • Emerging evidence supports the hypothesis that aberrant phase separation behavior may serve as a trigger of protein aggregation in neurodegeneration, and efforts to understand and control the underlying mechanisms are underway. (nih.gov)
  • Aberrant Liquid-liquid Phase Separation and Amyloid Aggregation of Pro" by Azeem Ahmad, Vladimir N. Uversky et al. (usf.edu)
  • A special issue of Separations (ISSN 2297-8739). (mdpi.com)
  • In the experimental part, the thesis describes work carried out to study the effects of stationary phase support properties on the chromatographic behaviour and enantiomer resolution capability of carbohydrate carbamate phases. (warwick.ac.uk)
  • The individual components are separated between a stationary phase (the tubular column in a column) and a mobile phase, which flows through the stationary packing (in this case a liquid). (burkert.com)
  • SFC is mainly decided by the stationary phase nature. (lu.se)
  • These membraneless structures behave as liquid droplets in the cytoplasm or nucleoplasm. (nature.com)
  • Motility of the phase separated domains leads to finite-size steady states, where coalescence of smaller droplets is balanced by the break-up of larger ones. (technion.ac.il)
  • Here we present experimental evidences in support of dimple coalescence between phase-separated liquid droplets in pure, supercritical, fluids under microgravity conditions. (epj.org)
  • We found that the number of droplets increases rapidly during the intermediate stage of phase separation. (epj.org)
  • These diverse materials may exhibit time-dependent structures under transient or out-of-equilibrium conditions resulting from for example self-assembly processes, phase transitions or in response to external fields, such as flow. (lu.se)
  • Fundamentally, multi-component mixture separations can be achieved by the selective permeability of a membrane substrate, either via imposed pressure or concentration gradient. (aiche.org)
  • Distillation is one of the oldest and most important separation processes used in the chemical and petrochemical industries. (scirp.org)
  • The emphasis is on understanding the separation processes involved and the instrumentation used, including different detection techniques. (lu.se)
  • The emphasis is on understanding the separation processes involved and the instrumentation used. (lu.se)
  • Both full view (macroscopic) and microscopic view images of the sample cell unit were analyzed to determine the changes in the distribution of liquid droplet diameters during phase separation. (epj.org)
  • G. Cordeiro, S. Dantas, L. Vasconcelos and R. Brito, "Effect of Two Liquid Phases on the Separation Efficiency of Distillation Columns," Advances in Chemical Engineering and Science , Vol. 3 No. 1, 2013, pp. 1-8. (scirp.org)
  • With water-assisted separation, we have shown that the wettability of a 3D-printed membrane can be tailored to enhance the oil-water separation efficiency by applying a hydrogel-coating on the membrane. (aiche.org)
  • To overcome the deficiencies in the current paradigm, a 3D-printed integrated membrane device capable of simultaneously separating multi-component and multi-phase mixture has been developed in our lab. (aiche.org)
  • By designing and modifying the internal structure of the integrated membrane device using computer-aided design (CAD), we have successfully enhanced the separation throughput. (aiche.org)
  • Our work suggests that the novel 3D-printed integrated membrane device has great potential in the next-generation manufacturing and separation industry. (aiche.org)
  • The separation of anionic arsenic compounds [3-nitro-4-hydroxyphenylarsonic acid (3-NHPAA), p-arsanilic acid (p-ASA) and phenylarsonic acid (PA)] was accomplished using 5 mM tetrabutylammonium chloride as the ion pairing reagent in aqueous mobile phase containing 3 mM malonic acid and 5% methanol at a flow rate of 1.4 mL minâ€"1. (utm.my)
  • Organic compounds and liquid water are major aerosol constituents in the southeast United States (SE US). (copernicus.org)
  • Water associated with inorganic constituents (inorganic water) can contribute to the partitioning medium for organic aerosol when relative humidities or organic matter to organic carbon (OM ∕ OC) ratios are high such that separation relative humidities (SRH) are below the ambient relative humidity (RH). (copernicus.org)
  • As OM ∕ OC ratios in the SE US are often between 1.8 and 2.2, organic aerosol experiences both mixing with inorganic water and separation from it. (copernicus.org)
  • Kinetics and mechanisms of the gas-phase reactions of the hydroxylradical with organic compounds under atmospheric conditions. (cdc.gov)
  • A structure-activity relationship for the estimation of rate constants for the gas-phase reactions of OH radicals with organic compounds. (cdc.gov)
  • A low-viscosity mobile phase consisting of compressed trial organic matter in geosciences because the composition carbon dioxide is used in SFC to achieve fast and efficient of its phenols is an important indicator of the type of original separation. (lu.se)
  • The first one is a gravity-fed separation of a multi-phase oil-water mixture. (aiche.org)
  • In phosphate based mobile phase, increasing the concentration of OSA and HSA increased the capacity factors (K) of all catecholamines (CAs) in the standard mixture and higher concentration of HSA was required to reach the same values. (medicaljournal-ias.org)
  • The ZK three-phase centrifuge can realize the three-phase continuous separation of two immiscible solutions and solids in one process, reducing the separation stage of the solid-liquid three-phase mixture, thereby greatly saving the production cost of the enterprise. (zkcentrifuge.com)
  • Previously, dimple coalescences were only observed in density-matched binary liquid mixture near its critical point of miscibility. (epj.org)
  • Currently, there is no NIOSH method or literature method for the separation and identification of the major components in a MWF mixture. (cdc.gov)
  • T. L. Junqueira, M. O. S. Dias, R. Maciel Filho, M. R. W. Maciel and C. E. V. Rossel, "Simulation of the Azeotropic Distillation for Anhydrous Bioethanol Production: Study on the Formation of a Second Liquid Phase," Computer Aided Chemical Engineering, Vol. 27, 2009, pp. 1143-1148. (scirp.org)
  • The separation of fullerenes and their derivatives on four commercially available reversed-phase columns was investigated using liquid chroma¬tography/mass spectrometry with atmospheric pressure chemical ionization. (canada.ca)
  • It differs from Level A in that it incorporates a non-encapsulating, splash-protective, chemical-resistant splash suit that provides Level A protection against liquids but is not airtight. (cdc.gov)
  • B. P. Guedes, M. F. Figueiredo, L. G. S. Vasconcelos, A. C. B. Araújo and R. P. Brito, "Sensitivity and Dynamic Behavior Analysis of an Industrial Azeotropic Distillation Column," Separation and Purification Technology, Vol. 56, No. 3, 2007, pp. 270-277. (scirp.org)
  • Moreover, the column was applied for egg white fast separation , further demonstrating its applicability for complex bio-sample separation . (bvsalud.org)
  • Indoor Air: Ammonia can be released into indoor air as a liquid spray (aerosol) or as a vapor. (cdc.gov)
  • Schematic picture of a hexagonal phase with anionic DNA and rodlike cationic CTA micelles which are distorted to reduce the electrostatic free energy. (lu.se)
  • groups bonded to the fullerene surface appear to disrupt adsorptive interactions between the fullerene molecular footprints and the stationary phases. (canada.ca)
  • Liquid-liquid phase separation facilitates the biogenesis of secretory storage granules. (nih.gov)
  • Y. Wu and I. Chien, "Design and Control of Heterogeneous Azeotropic Column System for the Separation of Pyridine and Water," Industrial & Engineering Chemistry Research, Vol. 48, No. 23, 2009, pp. 10564-10576. (scirp.org)
  • These databases contain manually curated data supported by experimental evidence in the literature and can include related features as presence of protein disorder, low complexity, post-translational modifications, experimental details, phase diagrams, among others. (wikipedia.org)
  • Due to the various velocities at which all liquids are pumped through the system, individual components can be separated based on the different flow properties and varying interactions between the stationary and the mobile phases of the substances. (burkert.com)
  • It was shown that the retention of solutes by these phases correlated directly with the w/w phase loading, whilst both the separation factor and resolution for various enantiomers displayed a more complex relationship. (warwick.ac.uk)
  • Phase separation has important implications for the mechanical, thermal, and electrical properties of materials. (epj.org)
  • Furthermore, the course aims to enable students to develop their ability to independently select and optimise a suitable separation technique/method and to present a coherent holistic view of the subject. (lu.se)
  • His research focuses on the design and development of advanced materials for use in separation, purification and sensor technologies. (uwm.edu)
  • In ammonium acetate based mobile phase, increasing the concentration of the ammonium acetate significantly reduced the K values of all CAs when HSA was used as an ionpairing agent. (medicaljournal-ias.org)
  • Alteration of the ammonium acetate concentration in PSA containing mobile phase had minimal effect on K values. (medicaljournal-ias.org)
  • In contrast to the results obtained with phosphate buffer, increasing the pH of the ammonium acetate based mobile phase resulted in an increase in the K values of all compounds investigated. (medicaljournal-ias.org)
  • The three-phase centrifuge's unique mechanism enables the solid-liquid-liquid phase separation and the discharge of the light and heavy liquid and solid phase separately. (zkcentrifuge.com)
  • Two distinct separation mechanisms were being utilized. (aiche.org)
  • The problem is that in mixed-phase clouds (supercooled liquid and ice) in principal both mechanisms can co-exist, and it is unclear which of the two prevail. (lu.se)
  • The reduction in the reboiler heat duty caused significant changes in concentration and temperature profiles throughout the column due to the formation of two liquid phases. (scirp.org)
  • M. Z. Lao and R. Taylor, "Modeling Mass-Transfer in 3-Phase Distillation," Industrial and Engineering Chemistry Research, Vol. 33, No. 11, 1994, pp. 2637-2650. (scirp.org)
  • Recently, a model for heterochromatin organization based on distinct liquid-like phase has been proposed. (emerging-researchers.org)
  • Agricultural: If ammonia is released into the air as a liquid spray (aerosol), it has the potential to contaminate agricultural products. (cdc.gov)