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ChromatographyChromatography, Thin LayerChromatography, High Pressure LiquidChromatography, LiquidChromatography, AffinityChromatography, GelChromatography, GasChromatography, Ion ExchangeIonic LiquidsMolecular WeightLiquid CrystalsMass SpectrometryCountercurrent DistributionChromatography, DEAE-CelluloseElectrophoresis, Polyacrylamide GelKineticsMolecular Sequence DataSolubilityGas Chromatography-Mass SpectrometryAmino Acid SequenceOctanolsTandem Mass SpectrometryChromatography, Reverse-PhaseChromatography, AgaroseHydrogen-Ion ConcentrationWaterAmino AcidsSpectrophotometry, UltravioletSpectrometry, Mass, Electrospray IonizationPhysicochemical Phenomena1-OctanolChemistry, PhysicalSolventsTemperatureCattleChemistryChemical FractionationEscherichia coliChemical PhenomenaChromatography, PaperSubstrate SpecificityReproducibility of ResultsMethodsProtein BindingMagnetic Resonance SpectroscopyPhosphatidylcholinesBacteriophage P1Bacterial ProteinsCalibrationIndicators and ReagentsReference StandardsLiverSepharoseLipid BilayersMolecular StructureDrug StabilityBase SequenceHydrolysisPeptidesRabbitsModels, BiologicalPeptide FragmentsBinding SitesCarbohydratesPhospholipidsAlgorithmsSpectrometry, FluorescenceMicrochemistryCell MembraneModels, ChemicalMembranes, ArtificialIsoelectric FocusingThermodynamicsPlasmidsMacromolecular SubstancesStereoisomerismLiposomesIsoelectric PointCarbohydrate SequenceMethanolChemistry Techniques, AnalyticalTime FactorsProteinsRecombinant ProteinsSpecies SpecificitySolutionsChromatography, Micellar Electrokinetic CapillarySensitivity and SpecificityOligosaccharidesFatty AcidsHydrophobic and Hydrophilic InteractionsStructure-Activity RelationshipLimit of DetectionTrypsinPolyethylene GlycolsDetergentsSolid Phase ExtractionPharmaceutical PreparationsPlant ExtractsSwine
Thin-layer chromatoHPLCQuantified by employing high perfCoefficientReverse-phase Liquid ChromatographyIonic LiquidsCoefficientsExchange chromatographyChromatographicStationary phaseCompoundsUHPLCAnalyticalAnalyteAcetonitrile followedVialsHydrophilicSelectivityPurificationVolatileCompositionPhasesAdsorptionHighDispersiveSolventsMolecularSeparation methodInteractionInteractionsExtractionColumnMassRetentionEttreDiversePaperJournalChemical
Thin-layer chromato2
HPLC7
  • Liquid chromatography generally uses small particles packed and operating at comparatively high pressure, and is denoted as high performance liquid chromatography (HPLC). (futuremarketinsights.com)
  • Modern Liquid Chromatography-Mass Spectrometry (LC-MS) methods use HPLC instrumentation, basically for sample introduction. (futuremarketinsights.com)
  • High performance liquid chromatography (HPLC) was performed by using the isocratic mobile phase, consisting of acetonitrile and water at a ratio of 67:33 with a flow rate of 1 mL/min and a run time of 18 min. (tubitak.gov.tr)
  • 14. Chromatography: detailed especially HPLC, HPTLC and other modern techniques and basic fundamental theory like van demeter equation, resolution, hetp etc. (pharmatutor.org)
  • To evaluate the described method, 21 pesticides belonging to different chemical classes were analysed using high performance liquid chromatography with diode-array detection (HPLC-DAD). (unboundmedicine.com)
  • The test principle for the CDC method utilizes high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for the quantitative detection of 25-hydroxyvitamin D3 (25OHD3), 3-epi-25-hydroxyvitamin D3 (epi-25OHD3), and 25-hydroxyvitamin D2 (25OHD2) in human serum. (cdc.gov)
  • Although it is widely known that proanthocyanidins elute at an increasing order of molecular masses when analysed by normal phase high performance liquid chromatography (NP- HPLC ), there is no a consistent explanation of the mechanisms of their separation until now. (bvsalud.org)
Quantified by employing high perf1
Coefficient14
  • The article describes both the theory in terms of the partition coefficient of a compound, and the application of the process to the separation of amino acids on a water-impregnated silica column eluted with a water:chloroform:n-butanol solvent mixture. (wikipedia.org)
  • Using this equation, the partition coefficient of thioglycolic acid has been determined to be 1.89 at 22°C (+ or - 0.5°C), or logP = 0.276. (europa.eu)
  • At pH 7, the partition coefficient was estimated to be 1.01*10e-3, so logP = -2.99. (europa.eu)
  • The partition coefficient of thioglycolic acid was evaluated in a study performed in accordance with OECD testing guideline 107 and GLP requirements. (europa.eu)
  • A partition coefficient study has been carried out with thioglycolic acid according to OECD 107. (europa.eu)
  • The measured partition coefficient for thioglycolic acid is -2.99 at 22°C. (europa.eu)
  • The partition coefficient of the test item can be obtained by interpolation of the calculated capacity factor on the calibration graph of the reference items. (europa.eu)
  • and OECD Test Guideline 117 an extrapolation of the partition coefficient to a value outside the calibration range is not recommended. (europa.eu)
  • The trainee focused on taking analogs of oleanolic acid and analyzing their Octanol-Water Partition Coefficient using High-Performance Liquid Chromatography. (ca.gov)
  • Because the test substance was a dissociative substance (dissociation constant: 10.53), its partition coefficient was determined using a buffered solution as solvent in the form of a non-dissociative substance (free base). (europa.eu)
  • The present study was conducted in order to obtain information on the 1-octanol/water partition coefficient of 1,3 -bis(Aminomethyl)Cyclohexane. (europa.eu)
  • The partition coefficient of 1,3-BAC has been determied to be 6.07 at 21.5±0.5°C, log Pow 0.783. (europa.eu)
  • The octanol-water partition coefficient of 99mTc tricarbonyl glycine oligomers indicated hydrophilic properties. (koreamed.org)
  • The partition coefficient n-octabol/water was determined to be log Pow = 4.4 at 25°C. (europa.eu)
Reverse-phase Liquid Chromatography1
  • In traditional Reverse-phase Liquid Chromatography (RPLC), polar, hydrophilic and charged compounds are preferentially retained on the column. (news-medical.net)
Ionic Liquids4
  • Crosslinked pyrrolidinium-based poly(ionic liquids) (Pyrr-PILs) were synthesized through a fast, simple, and solventless photopolymerization scheme, and tested as solid phase microextraction (SPME) sorbents. (ua.pt)
  • Ionic liquids have tunable selectivity for specific classes of analytes, giving them distinct advantages over organic solvents. (chromatographyonline.com)
  • This short review covers the use of ionic liquids (ILs) and polymeric ionic liquids (PILs) in solid-phase microextraction (SPME) and dispersive liquid-liquid microextraction (DLLME). (chromatographyonline.com)
  • Over the past decade, ionic liquids (ILs) and polymeric ionic liquids (PILs) have been widely studied and applied in multiple areas of science and engineering. (chromatographyonline.com)
Coefficients4
  • The proposed headspace analysis technique coupled with partition coefficients allows for a rapid and sensitive prediction of the volatile content in the liquid. (cdc.gov)
  • Tissue-to-blood partition coefficients ( R T ) ranged from 4.69 (muscle) to 41.4 (lungs). (aspetjournals.org)
  • Polymer-water partition coefficients (Kpw) of ten DDT-related compounds were determined in pure water at 25 °C using commercial polydimethylsiloxane-coated optical fiber. (usgs.gov)
  • This demonstrates the importance of determining partition coefficients under conditions as they exist in the field. (usgs.gov)
Exchange chromatography1
Chromatographic3
  • In contrast, Martin and Synge developed and described a chromatographic separation process whereby compounds were partitioned between two liquid phases similar to the separatory funnel liquid-liquid separation dynamic. (wikipedia.org)
  • It is the adaptability of paper chromatography in its numerous varieties that is behind its inescapable remaining in division science, combined with simplicity of approach and a sensibly all around created outline in which the different chromatographic procedures work. (tropicalnaturetravel.com)
  • The territory of scattering limits the limit of the chromatographic framework to separate and, autonomous of great thermodynamic commitments to the partition, there is a limited number of scattered zones which could be obliged from the detachment. (tropicalnaturetravel.com)
Stationary phase11
  • The process of separating mixtures of chemical compounds by passing them through a column that contains a solid stationary phase that was eluted with a mobile phase (column chromatography) was well known at that time. (wikipedia.org)
  • Liquid chromatography (LC) separates molecules in a liquid mobile phase by means of a solid stationary phase. (futuremarketinsights.com)
  • Chromatography, called a physical separation method in which the components of a mixture are separated by differences in their distribution between two phases, one of which is static stationary phase while the other mobile phase moves through it in a particular direction, is a mainstay of forensic procedures and evaluations. (ringstilsoldout.com)
  • Within the column, the gears become divided by differential divider between the mobile phase petrol and stationary phase liquid. (ringstilsoldout.com)
  • Hydrophilic interaction chromatography (HILIC) is a liquid chromatography technique that is used to separate moderate to highly hydrophilic and polar compounds based on their retention on a hydrophilic stationary phase versus the hydrophilic aqueous polar organic mobile phase. (news-medical.net)
  • This is in contrast to the retention mechanism in HILIC, which relies on the partitioning of a compound between the aqueous surface layer of the hydrophilic stationary phase and the organic, hydrophilic compartment of the mobile phase. (news-medical.net)
  • Secondary forces, such as dipole-dipole, electrostatic, and hydrogen bonding interactions influence this partition, resulting in retention of the compound on the stationary phase or elution in the mobile phase. (news-medical.net)
  • The stationary phase separates the analyte to be measured from other molecules of a different type present in the mixture based on differential partitioning between the mobile and stationary phases (affinity). (paduiblog.com)
  • Gas chromatography (GC) is based on a partition equilibrium of analyte between a solid stationary phase (often a liquid silicone-based material) and a mobile gas (most often Helium). (paduiblog.com)
  • Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. (bvsalud.org)
  • Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. (bvsalud.org)
Compounds4
  • Volatile organic compounds (VOCs) were screened from a convenience sample of 146 e-liquids by equilibrating 1 g of each e-liquid in amber vials for 24 h at room temperature. (cdc.gov)
  • Headspace was transferred to an evacuated canister and quantitatively analyzed for 20 VOCs as well as tentatively identified compounds using a preconcentrator/gas chromatography/ mass spectrometer system. (cdc.gov)
  • Chromatography methods are used to organic and inorganic compounds such as separate proteins, nucleic acids, or small molecules in complex mixtures and others. (futuremarketinsights.com)
  • Gas chromatography/mass spectroscopy analysis showed that the extract contained monoterpene hydrocarbons and phenolic compounds, the major ones being carvacrol and thymol and to a lesser extent p-cymene, 1-octacosanol, creosol, and phytol. (hindawi.com)
UHPLC1
  • SwRI's instrumentation for GLP 5-batch analysis includes a triple quadrupole (QQQ) mass spectrometer (TQMS) with an ultra-high performance liquid chromatography (UHPLC) separation module. (swri.org)
Analytical5
  • Finally, gas-liquid chromatography, a fundamental technique in modern analytical chemistry, was described by Martin with coauthors A. T. James and G. Howard Smith in 1952. (wikipedia.org)
  • An analytical method was developed that provided quantitative and qualitative chemical information for characterizing the volatile constituents of bulk electronic cigarette liquids (e-liquids) using a static headspace technique. (cdc.gov)
  • Liquid chromatography can be used for analytical or preparative applications. (futuremarketinsights.com)
  • This method can be preparative or analytical, wherein preparative chromatography isolates chemical for further analytical and research is concerned with measuring relative proportions. (ringstilsoldout.com)
  • It is a relatively 'new' form of analytical chemistry in that the first true use of modern chromatography is usually attributed to Russian botanist Mikhail Semyonovich Tsvet, who used columns of calcium carbonate for separating plant pigments during the first decade of the 20th century during his research of chlorophyll. (paduiblog.com)
Analyte4
  • Chromatography might be utilized as a refinement strategy or for estimating analyte extents in a combination. (brandywinerollergirls.com)
  • An analyte is the genuine substance being submitted to the partition cycle. (brandywinerollergirls.com)
  • When SPME is coupled to gas chromatography (SPME-GC), the IL- or PIL-based sorbent materials must be capable of withstanding the high operating temperatures of the GC injector (250-280 °C) during analyte desorption (5). (chromatographyonline.com)
  • In GC analysis, a known volume of gaseous or liquid analyte is injected into the "entrance" (head) of column usually using a micro-syringe. (foodtechnotes.com)
Acetonitrile followed2
  • The samples were processed by extraction with acetonitrile followed by sonication and liquid-liquid partition. (tubitak.gov.tr)
  • The proposed method is based on liquid-liquid partitioning with acetonitrile followed by dispersive solid phase extraction using zirconia-coated silica particles for extract purification. (unboundmedicine.com)
Vials1
  • A convenience sample of 26 bulk liquid flavorings obtained from two coffee roasting and packaging facilities in the USA was analyzed for 20 volatile organic chemicals present in the headspaces of vials containing flavoring liquids using gas chromatography-mass spectrometry. (cdc.gov)
Hydrophilic1
  • This contrasts with the order seen in standard reverse-phase chromatography, where elution occurs in the order of least to most hydrophilic. (news-medical.net)
Selectivity1
  • We introduce two new dedicated certified reference materials (CRMs) and their analysis by GC using an ionic liquid capillary column, providing unique selectivity & stability. (sigmaaldrich.com)
Purification1
  • Ionic-liquid-based aqueous biphasic systems (IL-based ABS) have been largely investigated as promising extraction and purification routes. (ua.pt)
Volatile1
  • Static headspace screening of e-liquids allows for the identification of volatile chemical constituents which is critical for identifying and controlling emission of potentially hazardous constituents in the workplace. (cdc.gov)
Composition1
Phases7
  • The seminal article presenting their early studies described a rather complicated instrument that allowed partitioning of amino acids between water and chloroform phases. (wikipedia.org)
  • This work was published in 1941 as "a new form of chromatogram employing two liquid phases. (wikipedia.org)
  • The original problem that Martin and Synge encountered with devising an instrument that would employ two free-flowing liquid phases was solved by Lyman C. Craig in 1944, and commercial counter-current distribution instruments were used for many important discoveries. (wikipedia.org)
  • A new form of chromatogram employing two liquid phases. (wikipedia.org)
  • Liquid chromatography procedure can be directed in various combination of possible mobile phases and stationary phases. (futuremarketinsights.com)
  • Ordinarily, the use of gas chromatography in a laboratory setting involves passing a mixture through a series of phases. (ringstilsoldout.com)
  • Collaborations are essentially physical in kind or include powerless substance bonds, for example, dipole-dipole, hydrogen bond development, charge transport, and so on, and reversible, on the grounds that valuable partitions possibly result if the synthetic invests some energy in the two phases. (tropicalnaturetravel.com)
Adsorption1
  • However, separation of monomer species, namely catechins and some hydroxybenzoic acids , was based on classic adsorption /partition mechanism. (bvsalud.org)
High3
  • During the pandemic, high-performance, liquid chromatography techniques became a reliable component for developing life-saving vaccines and other disease-detecting systems. (futuremarketinsights.com)
  • Labeling yields of 99mTc-tricarbonyl glycine monomers and oligomers were checked by high-performance liquid chromatography. (koreamed.org)
  • Why proanthocyanidins elute at increasing order of molecular masses when analysed by normal phase high performance liquid chromatography? (bvsalud.org)
Dispersive1
  • ILs and PILs have been previously applied in various extraction methods including single-drop microextraction (SDME), liquid-phase microextraction (LPME), solid-phase microextraction (SPME), dispersive liquid-liquid microextraction (DLLME), hollow fiber-supported liquid membrane extraction (HFSLME), and solid-phase extraction (SPE) (2-7). (chromatographyonline.com)
Solvents2
Molecular1
  • The MIPs are produced by various polymerization methods but in all cases specifi c interactions, molecular partitioning and polymer structure are key properties that we focus on. (lu.se)
Separation method1
  • Paper chromatography is the most broadly utilized Separation method in synthetic labs , where it is utilized in investigation, detachment and filtration, and it is regularly used in the compound cycle industry as a piece of little and enormous scope producing. (tropicalnaturetravel.com)
Interaction1
  • Chromatography is a logical interaction comprising of numerous research facility procedures used to isolate a blend. (brandywinerollergirls.com)
Interactions1
  • Irrespective of the interactions that are being brought, liquid column chromatography is taken up in six steps: Column equilibration, Sample loading, Washing, Elution, Final column washing and Column regeneration. (futuremarketinsights.com)
Extraction3
  • Martin and Synge initially attempted to devise a method of performing a sequential liquid-liquid extraction with serially connected glass vessels that functioned as separatory funnels. (wikipedia.org)
  • The process was termed "counter-current liquid-liquid extraction. (wikipedia.org)
  • Analytes are extracted from the aqueous phase into the hexane layer (liquid-liquid extraction), which is then dried under vacuum. (cdc.gov)
Column3
Mass3
  • Single oral and intravenous doses of FTY720 were administered to male Wistar rats, with blood and tissue sampling over 360 h analyzed by liquid chromatography/tandem mass spectrometry. (aspetjournals.org)
  • The liquid chromatograph/tandem mass spectrometer was obtained through a shared instrumentation grant (S10RR14573) from the National Center for Research Resources, National Institutes of Health. (aspetjournals.org)
  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based measurements for PRA and PAC have become popular. (degruyter.com)
Retention1
Ettre1
  • Leslie Ettre remembers A.J.P. Martin, inventor of partition, paper, reversed-phase, and gas–liquid partition chromatography. (chromatographyonline.com)
Diverse1
Paper2
  • Paper Chromatography is in a general sense an actual arrangement of detachment where the segments of a blend are isolated by their dispersion between two stages while the other is a liquid which permeates through or over the static stage. (tropicalnaturetravel.com)
  • Paper Chromatography is recognized by their ability to partake in like manner intermolecular connections from the two stages, which may regularly be portrayed by a harmony steady, and is accordingly a land called from compound thermodynamics. (tropicalnaturetravel.com)
Journal3
Chemical1