• Nano-flow liquid chromatography tandem mass spectrometry (nano-flow LC-MS/MS) is the mainstay in proteome research because of its excellent sensitivity but often comes at the expense of robustness. (nature.com)
  • 120,000 peptides in 16 h and sample multiplexing using tandem mass tags increases throughput to 11 proteomes in 16 h. (nature.com)
  • A proteomics informed by transcriptomics (PIT) approach was applied to analyse samples of female and male saliva separately using the previously obtained O. erraticus sialotranscriptome as a reference database and two different mass spectrometry techniques, namely liquid chromatography-tandem mass spectrometry (LC-MS/MS) in data-dependent acquisition mode and sequential window acquisition of all theoretical fragment ion spectra MS (SWATH-MS). (biomedcentral.com)
  • Liquid chromatography and tandem mass spectrometry (LC-MS/MS) were performed. (datadryad.org)
  • This method uses online solid phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry (SPE-HPLC-MS/MS) for quantifying DEET and two of its metabolites, N,N-diethyl-3-hydroxymethylbenzamide (DHMB) and 3-diethyl-carbamoyl benzoic acid (DCBA), in 100 µL of human urine. (cdc.gov)
  • Proteomic assessment of physiological disorder syndromes in longan ( Dimocapus longan 'Daw') on mature stage using one-dimensional electrophoresis coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS), the 5th Asia Pacific Protein Association Conference and 12th PST International Symposium. (mju.ac.th)
  • Using gel electrophoresis followed by liquid chromatography coupled with tandem mass spectrometry, we aimed to characterize the proteome of EVs released by the adult O. felineus liver fluke. (bvsalud.org)
  • Nonvolatile compounds were identified by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). LC-MS/MS was operated in negative mode with 0.1 % formic acid in water and acetonitrile as mobile phase. (ui.ac.id)
  • The current study describes the implementation of a hybridization liquid chromatography-tandem mass spectrometry (LC-MS/MS) platform for the direct quantitation of antisense oligonucleotide (ASO) ƒ u . (aspetjournals.org)
  • Liquid chromatography-tandem mass spectrometry (LC-MS) is effective for the analysis of lipids in biological samples. (biomedcentral.com)
  • Simultaneous determination of berberine, palmatine and jatrorrhizine by liquid chromatography-tandem mass spectrometry in rat plasma and its application in a pharmacokinetic study after oral administration of coptis-evodia herb couple[J]. J Chromatogr B, 2008, 863(2):195-205. (magtechjournal.com)
  • In addition, gas chromatography, infrared spectroscopy and combined gas chromatography/mass spectrometry were applied for identification purposes. (niom.no)
  • The first proteomics experiment was conducted with the invention of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). (wikipedia.org)
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  • Proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were transferred onto a polyvinylidene difluoride membrane at 100 V for 1.5 hr. (cdc.gov)
  • Analytical Techniques (Chromatography, Spectroscopy and Electrophoresis) in Pharmaceutical analysis: A Review. (ajrconline.org)
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  • Principle, Instrumentation and Applications of UPLC: A Novel Technique of Liquid Chromatography. (ajrconline.org)
  • Lipidomics based on UPLC combined with quadrupole-Exactive Orbitrap mass spectrometry (UPLC-Q-Exactive MS) has been widely applied to obtain insight into many biological events [ 10 ]. (biomedcentral.com)
  • The current proteomics of microbial community makes use of both gel-based (one-dimensional and two-dimensional) and non-gel liquid chromatography based separation, where both rely on mass spectrometry based peptide identification. (wikipedia.org)
  • A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (lookformedical.com)
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  • Fractionations of the extract were performed by using gravity column chromatography on silica gel and Sephadex LH-20 as stationary phase. (ui.ac.id)
  • The fully hydrated form of silica gel has distinct properties and is referred to as SILICIC ACID. (lookformedical.com)
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  • Over the years, technologic advances in electronics and detectors have allowed GC/MS to detect ever smaller concentrations of compounds, as has the addition of other analytical techniques, such as liquid chromatography. (medscape.com)
  • The research aims to determine antioxidant activity of the gel form of red algae ethanol extract by DPPH (1,1-diphenyl-2-picrylhydrazil) method. (rjptonline.org)
  • Rather, mass photometry, crosslinking mass spectrometry, single molecule TIRF microscopy and biochemical assays identify inter-holoenzyme phosphorylation (IHP) as the mechanism for spreading phosphorylation. (elifesciences.org)
  • Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. (lookformedical.com)
  • A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. (lookformedical.com)
  • It is commonly used as a desiccating agent and as a stationary phase for CHROMATOGRAPHY. (lookformedical.com)
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  • This technique is based on the dispersion of a solid sorbent in liquid samples in the extraction isolation and clean-up of different analytes from complex matrices. (hindawi.com)
  • A large number of urine analytes can be assessed from the dried urine with similar results to those from liquid urine. (biomedcentral.com)
  • Absolute quantification by mass spectrometry of HlgCB in 39 community-acquired pneumonia (CAP) isolates showed considerable variations in the HlgC and HlgB yields between isolates. (cnrs.fr)
  • Sequential window acquisition of all theoretical fragment ion spectra (SWATH) is a label-free mass spectrometry quantitative technique, which can retain complete fragment ion data and has good accuracy as well as reproducibility. (ncl.edu.tw)
  • A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds. (lookformedical.com)
  • Trypsin digestion was stopped with 10% trifluoroacetic acid (TFA) and the supernatant (SN) was removed, then the library gel slides were dehydrated with pure acetonitrile (ACN). (datadryad.org)
  • Characterization of an oligomeric compound was performed using gel permeation liquid chromatography. (niom.no)
  • 3. Shrotriya, S. Ranpise, N. Satpute, P. Vidhate, B. Skin Targeting of Curcumin Solid Lipid Nanoparticles-Engrossed Topical Gel for the Treatment of Pigmentation and Irritant Contact Dermatitis. (rjptonline.org)
  • The method provides substantial improvements, including minimal matrix effects and high specificity when compared with previously used oligonucleotide ƒ u detection methods such as ligand binding assays or liquid scintillation. (aspetjournals.org)
  • The hybridization LC-MS/MS platform provided unique advantages, including minimal matrix effects and high specificity, compared with traditional ligand binding assays or liquid scintillation approaches, which enabled efficient and reliable in vitro protein binding assay. (aspetjournals.org)
  • High-performance liquid chromatography with diode array detection system was used for identification and quantification of the monomers in the resin systems. (niom.no)
  • Peptides were analyzed in a mass spectrometer nanoESI qQTOF (5600 TripleTOF, ABSCIEX). (datadryad.org)
  • The aims of this study were, first, to confirm that mass spectrometric analysis of an array of hormones and organic acids would yield similar results in both liquid and dried urine, and, second, to determine if collection of four dried spot urine samples could be substituted for a 24-h collection when measuring reproductive hormones. (biomedcentral.com)
  • Twenty individuals collected both a spot liquid urine and dried urine on filter paper to analyze eight organic acids. (biomedcentral.com)
  • data from 18 of these individuals were available to compare liquid versus dried urine results. (biomedcentral.com)
  • comparison of dried to liquid urine for organic acids showed good to excellent agreement (ICC range: 0.75 to 0.99). (biomedcentral.com)
  • The first therapeutic use of steroids occurred in the 18th century when English physician William Withering used digitalis, a compound extracted from the leaves of the common foxglove Digitalis purpurea , to treat edema, donde comprar estanozolol en peru var kan man köpa anabola steroider. (mrcatholic.biz)
  • Agonists, such as clenbuterol and albuterol, increase muscle strength and muscle mass through their influ- ence on muscle protein metabolism and contractile. (dispatchameertransportation.org)
  • Every year, approximately one-third of the U.S. population uses DEET-containing insect repellent products with concentrations ranging from 10 to 100% in a variety of liquids, lotions, gels, sprays, sticks and impregnated materials and more than 30 million packages of DEET-containing products are sold annually. (cdc.gov)
  • Stability studies of gel have carried out for 21 days at viscosity and an antioxidant activity using the DPPH method. (rjptonline.org)
  • This might be the main reason why Deca is really a suppressive drug if this involves your natural testosterone levels, how to cycle clenbuterol liquid. (maliekakids.com)
  • In 2017, the most recent data available as of this writing, 73,990 individuals died of drug-induced causes in the United States, with men (n = 48,967) dying from these causes nearly twice as much as women (n = 25,023), and non-Hispanic White men and women dying significantly more from these causes than other races/ethnicities. (medscape.com)
  • Antisense oligonucleotides (ASOs), defined as short synthetic oligonucleotides with single-stranded sequences complementary to certain mRNA sites, have been under drug development for approximately 30 years, with the first FDA-approved drug, Fomivirsen, being approved in 1998 ( Stein and Castanotto, 2017 ). (aspetjournals.org)
  • The Solar Eclipse of 2017 Had a Significant Effect on America's Drug Use. (murraystate.edu)
  • Taking D-Bal Max for more muscle mass is incredibly simple, how to cut stacks of paper without a paper cutter. (maliekakids.com)
  • Deaths related to drug overdoses in celebrities have brought mass media attention to the drug abuse pandemic. (medscape.com)
  • The eluting molecular ions are converted to gas phase ions using Atmospheric Pressure Chemical Ionization (APCI) and then selectively filtered by mass-to-charge ratios at unit resolution. (cdc.gov)