Liquid and solid phase extraction solid phase extraction. Medical search. Frequent questions

Dr. Thiangthum and Dr. Tanunkat from Bureau of Drug and Narcotic of Thailand use the VERSA 1100 automated Solid Phase Extraction system to quantify amphetamine and methamphetamine in drug users. (aurorabiomed.com)
Download the validation poster to see how the VERSA 1100 automated Solid Phase Extraction system is eliminating cross-contamination while ensuring accuracy and reproducibility in urine samples of drug users. (aurorabiomed.com)
Target analytes are extracted and concentrated from the urine matrix using an automated solid phase extraction system. (cdc.gov)

Simultaneous determination of nine estrogens in bullfrogs using filtered solid phase extraction and ultra-performance liquid chromatography-tandem mass spectrometry]. (bvsalud.org)
A method is described for the determination of selamectin in dog plasma, using High-Performance Liquid Chromatography (HPLC) with fluorescence detection (excitation and emission wavelengths fixed at 355 and 465 nm, respectively). (vetres.org)
In this study, a selective and reliable method has been developed for the determination of ketoprofen in water and artificial serum using molecularly imprinted polymers (MIPs) as a solid phase extraction sorbent prior to HPLC-DAD detection. (tubitak.gov.tr)
A solid-phase extraction-liquid chromatographic-tandem mass spectrometry method for the determination of nalbuphine concentrations in human plasma has been developed. (unboundmedicine.com)
The nanocomposite was successfully used as magnetic solid phase extraction (MSPE) adsorbents for the determination of aflatoxins in edible vegetable oils through the π-π stacking interactions. (eurekamag.com)
Owing to the simplicity, rapidity and efficiency, Fe3O4/rGO magnetic solid phase extraction coupled with high-performance liquid chromatography fluorescence with post-column photochemical derivatization (Fe3O4/rGO MSPE-HPLC-PCD-FLD) is a promising analytical method for routine and accurate determination of aflatoxins in lipid matrices. (eurekamag.com)

SPE uses the affinity of solutes, dissolved or suspended in a liquid (known as the mobile phase), to a solid packing inside a small column, through which the sample is passed (known as the stationary phase), to separate a mixture into desired and undesired components. (wikipedia.org)
The result is that either the desired analytes of interest or undesired impurities in the sample are retained on the stationary phase. (wikipedia.org)
The portion that passes through the stationary phase is collected or discarded, depending on whether it contains the desired analytes or undesired impurities. (wikipedia.org)
If the portion retained on the stationary phase includes the desired analytes, they can then be removed from the stationary phase for collection in an additional step, in which the stationary phase is rinsed with an appropriate eluent. (wikipedia.org)
In the case of an incomplete extraction, the analytes do not have enough affinity for the stationary phase and part of them will remain in the permeate. (wikipedia.org)
SPE is in fact a method of chromatography, in the sense of having a mobile phase, carrying mixtures through a stationary phase, packed inside a column. (wikipedia.org)
The chromatographic process is harnessed to be an extractive technique, a solid-liquid extractive technique-taking advantage of large differences in the Keq, or equilibrium constant, of mixture components between the solid stationary phase and the mobile phase resulting, for a well-designed and executed separation. (wikipedia.org)
As the sample passes through the stationary phase, the polar analytes in the sample will interact and retain on the polar sorbent while the solvent, and other non-polar impurities pass through the cartridge. (wikipedia.org)
A stationary phase of polar functionally bonded silicas with short carbons chains frequently makes up the solid phase. (wikipedia.org)
This stationary phase will adsorb polar molecules which can be collected with a more polar solvent. (wikipedia.org)
The stationary phase of a reversed phase SPE cartridge is derivatized with hydrocarbon chains, which retain compounds of mid to low polarity due to the hydrophobic effect. (wikipedia.org)
The analyte can be eluted by washing the cartridge with a non-polar solvent, which disrupts the interaction of the analyte and the stationary phase. (wikipedia.org)
A stationary phase of silicon with carbon chains is commonly used. (wikipedia.org)
Ion exchange sorbents separate analytes based on electrostatic interactions between the analyte of interest and the positively or negatively charged groups on the stationary phase. (wikipedia.org)
SFC is mainly decided by the stationary phase nature. (lu.se)

Separation of the analytes was obtained using a mobile phase of 0.1 M sodium dihydrogen phosphate-acetonitrile (87:13, v/v) and a muBondapak C18 column. (hacettepe.edu.tr)
In preliminary experiments with a standard nanoLC-MS set-up (i.e. trap column for extraction + separation column), injecting protein precipitated pooled human plasma clogged the column(s) (see Fig. 1A ). (nature.com)
Selective separation of the analytes is achieved using high-performance liquid chromatography with a gradient elution program. (cdc.gov)
Chromatographic separation of nalbuphine and naloxone (internal standard) was achieved on a Phenomenex Kinetex PFP (2.6 μm, 100 A, 100 × 2.1 mm) column using a mobile phase consisting of 0.1% formic acid, 15 mM ammonium acetate in deionized water and acetonitrile (60:40, v/v). The flow rate was 0.3 mL/min and the total run time was 2 min. (unboundmedicine.com)
A low-viscosity mobile phase consisting of compressed trial organic matter in geosciences because the composition carbon dioxide is used in SFC to achieve fast and efficient of its phenols is an important indicator of the type of original separation. (lu.se)

The liquid chromatographic conditions were optimized, and the 0.5 mmol/L ammonium fluoride aqueous solution -acetonitrile system as mobile phases showed better sensitivity than the ammonium acetate aqueous solution -acetonitrile system and the ammonia -acetonitrile system for the nine estrogens . (bvsalud.org)

The preparation step used for measuring heptachlor epoxide in blood and serum involves lipid extraction, clean-up with column chromatography, and elution with acetonitrile, hexane, and methylene chloride (Burse et al. (cdc.gov)
Finally, the sample was extracted using acetonitrile, purified by PRiME HLB SPE cartridge, and separated on a Waters Acquity UPLC BEH C18 column (100 mm×2.1 mm, 1.7 µm) with a mobile phase of 0.5 mmol/L ammonium fluoride aqueous acetonitrile solution at a flow rate of 0.3 mL/min. (bvsalud.org)
Chromatography was performed on a C(18) reversed-phase column using a linear gradient of 10mM ammonium bicarbonate, pH 7.3 and acetonitrile as a mobile phase. (erowid.org)
The proposed method is based on liquid-liquid partitioning with acetonitrile followed by dispersive solid phase extraction using zirconia-coated silica particles for extract purification. (unboundmedicine.com)

Sample preparation steps for adipose tissue vary but, in general, involve a lipid extraction step followed by a clean-up procedure involving gel permeation chromatography (GPC) and/or Florisil column clean-up. (cdc.gov)
Automatic solid phase extraction using the benchmate procedure was used for sample preparation. (vetres.org)
The influence of several variables on the efficiency of the extraction procedure was investigated by single-factor experiments. (hindawi.com)
The proposed procedure demonstrated clear reductions in extraction time and elimination of cleanup steps. (hindawi.com)
MDMA and MDE were extracted from serum and homogenized brain structures using a solid-phase extraction procedure. (erowid.org)
The extracts were examined by a validated high-performance liquid chromatography procedure coupled with fluorimetric detection. (erowid.org)
Sample preparation involved a solid-phase extraction procedure at pH 6 of both non-hydrolyzed and enzymatically hydrolyzed urine samples. (erowid.org)

It is possible to have an incomplete recovery of the analytes by SPE caused by incomplete extraction or elution. (wikipedia.org)

Therefore, a rapid and accurate method based on PRiME HLB solid phase extraction (SPE) cartridge purification and ultra-performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS) was developed to determine nine estrogen residues in bullfrogs . (bvsalud.org)
In summary, the method used solid-phase extraction followed by liquid chromatography coupled to tandem mass spectrometry. (cdc.gov)
Passive sampling (Chemcatcher® with a HLB receiving phase) together with high-resolution tandem mass spectrometry-targeted screening was used to provide high sensitivity and selectivity for the identification of a wide range of emerging pollutants in these urban waters. (springer.com)
Nano-flow liquid chromatography tandem mass spectrometry (nano-flow LC-MS/MS) is the mainstay in proteome research because of its excellent sensitivity but often comes at the expense of robustness. (nature.com)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based measurements for PRA and PAC have become popular. (degruyter.com)
Zhang Y, Wang Q, Zhang G, Jia W, Ren Y, Wu Y. Biomarker analysis of hemoglobin adducts of acrylamide and glycidamide enantiomers for mid-term internal exposure assessment by isotope dilution ultra-high-performance liquid chromatography tandem mass spectrometry. (cdc.gov)
Urine samples from both patients were analysed using liquid-chromatography tandem mass spectrometry (LC-MS/MS) following glucuronidase digestion and solid-phase extraction. (erowid.org)

To evaluate the described method, 21 pesticides belonging to different chemical classes were analysed using high performance liquid chromatography with diode-array detection (HPLC-DAD). (unboundmedicine.com)

The authors developed a two-cartridge, solid-phase extraction method for isolating 12 SU, 3 IMI, and 1 SA herbicides by using high-performance liquid chromatography/electrospray ionization-mass spectrometry (HPLC/ESI-MS) to identify and quantify these herbicides to 10 ng/l. (usgs.gov)

This paper reports a rapid method for the isolation and purification of glucoraphan in from broccoli seeds using solid phase extraction (SPE) and preparative HPLC based on a method developed in our laboratories for the quantification of glucoraphanin in broccoli seeds and broccoli florets. (edu.au)

Although detection of TCs can be accomplished by liquid chromatography coupled with mass spectrometry, the use of this methodology requires costly instrumentation that is not always available in routine laboratory analyses, and applications to complex matrices such as manure do not always give good results [ 8 , 9 ]. (hindawi.com)

These metabolites underwent extensive phase II metabolism to yield sulfate and glucuronic acid conjugates. (cdc.gov)

The analytes were determined using high-performance liquid chromatography with ultraviolet detector. (hindawi.com)

In this study, the solid-phase extraction/quick, easy, cheap, effective, rugged and safe (SPE/QuEChERS) technique was adapted to develop a simple sample treatment for multi-residue pesticide analysis of edible oils. (unboundmedicine.com)

First, the cartridge is equilibrated with a non-polar or slightly polar solvent, which wets the surface and penetrates the bonded phase. (wikipedia.org)
VERSA automated Solid Phase Extraction systems (also known as automated SPE systems) fully automate SPE workflows from cartridge conditioning to sample derivatization. (aurorabiomed.com)
In the process, generally, solid phase extraction consumables refer to the extraction cartridge (including the filler), disk and 96 well plate etc. (reportsnreports.com)
And extraction cartridge is the most widely used in solid phase extraction SPE. (reportsnreports.com)

We describe a robust nano liquid chromatography-mass spectrometry (nanoLC-MS) platform for measuring the total amount of OT in human plasma/serum. (nature.com)

Concentrations of bupivacaine in plasma were determined by high-performance liquid chromatography. (vin.com)

A rapid and cleanup-free ultrasound-assisted extraction method is proposed for the simultaneous extraction of oxytetracycline, tetracycline, chlortetracycline, and doxycycline in manure. (hindawi.com)

Leak testing equipment is used to measure the escape of liquids, vacuum or gases from sealed components or systems. (globalspec.com)

This study optimized the mobile phase system, extraction solvent , and SPE cartridges. (bvsalud.org)
MSPE parameters including the amount of adsorbents, extraction and desorption time, washing conditions, and the type and volume of desorption solvent were optimized. (eurekamag.com)

Solid-phase extraction (SPE) is a solid-liquid extractive technique, by which compounds that are dissolved or suspended in a liquid mixture are separated, isolated or purified, from other compounds in this mixture, according to their physical and chemical properties. (wikipedia.org)
The differences are clear enough to differentiate SPE as an extractive technique, with theory, procedures, and aims derived from liquid chromatography, therefore, it has a unique niche in modern chemical science. (wikipedia.org)
Ersoz, A., Diltemiz, S.E., Ozcan, A.A. and Denizli, A. (2008) Synergie between Molecular Imprinted Polymer Based on Solid-Phase Extraction and Quartz Crystal Microbalance Technique for 8-OHdG Sensing. (scirp.org)

Rapid sample preparation was achieved by solid-phase extraction columns (Bond Elut CBA, 3 ml capacity) which provided excellent recovery values for both compounds. (hacettepe.edu.tr)
The maximum extraction capacity obtained was 15.46 mg Co/g of nanofiber and 70.15 mg Co/g of extractor. (open.ac.uk)

The temperature, pH, and amount of extraction solution were selected for optimization experiment using response surface methodology. (hindawi.com)
4.06%) under optimal conditions, which include 50 mL of McIlvaine buffer extraction solution (pH 7.15) mixed with 1 g of manure sample, extraction temperature of 40°C, extraction time of 10 min, and three extraction cycles. (hindawi.com)

Analytical laboratories use solid phase extraction to concentrate and purify samples for analysis. (wikipedia.org)
Conductivity meters, dissolved solids meters, and resistivity meters are analytical instruments that measure the conductivity, dissolved solids, and/or resistivity of a liquid sample. (globalspec.com)
Dissolved CO2 instruments are analytical devices that measure the amount of carbon dioxide (CO2) dissolved in a liquid sample such as water. (globalspec.com)
Dissolved oxygen meters are analytical instruments that are used to measure the amount of oxygen dissolved in a liquid sample. (globalspec.com)

Pressurized fluid extractor used to extract organic compounds from solid samples. (usgs.gov)
UHPSFC , ultra- pacted by other parameters, such as the addition of the mod- high performance supercritical fluid chromatography ifier and the change of the mobile phase density [20]. (lu.se)

This work studied the production of novel nanofibers modified with Cyanex 272 and their application in the recovery of cobalt present in aqueous solution The nanofibers produced by forcespinning were characterized by SEM, FT-IR, and TGA and the extraction of cobalt was evaluated by variation of the pH, solid:liquid (S:L) ratio, extraction time and Cyanex 272 content in the nanofibers. (open.ac.uk)
The findings showed that Nylon 6/Cyanex 272 nanofibers are a new robust and promising material for the recovery of heavy metals from aqueous solution, confirming that nanofibers have an efficiency similar to conventional liquid-liquid extraction, without the disadvantage of volatile organic compounds emissions generated by the use of organic diluents. (open.ac.uk)

The use of solid-phase extraction allows the use of robotic or manual sample preparation for the efficient clean-up of terfenadine and terfenadine acid metabolite from plasma. (nih.gov)
Solid Phase Extraction (SPE) is one of the most used sample preparation techniques in laboratories throughout the world. (coleparmer.com)
Select from Cole-Parmer TELOS sample preparation solid-phase extraction (SPE) columns and 96-well plates, and SLE plates. (coleparmer.com)
Solid phase extraction SPE is the sample preparation process. (reportsnreports.com)

A typical solid phase extraction involves five basic steps. (wikipedia.org)

A molecularly imprinted polymer as solid phase extraction sorbent for " by ELİF GÜREL ÖZYURT, ÖMER ÖZYURT et al. (tubitak.gov.tr)
Critical extraction parameters such as sample pH, shaking time and sorbent amount were optimized and adjusted to 8.0, 24 h, and 10.0 mg, respectively, for a sample volume of 10.0 mL. (tubitak.gov.tr)

Samples were assayed by high performance liquid chromatography and pharmacokinetic parameters were calculated from data plotted to the best-fit curve for samples from 15 to 180 min. (vin.com)

Samples (1 mL) were extracted using a Strata™-X solid phase extraction cartridges. (unboundmedicine.com)

Because estrogens present weak alkalinity, adding a small amount of alkaline substance to the mobile phase benefits estrogen ionization into the ionic state , eliminates the peak trailing phenomenon, and enhances the signal response of estrogens to improve sensitivity . (bvsalud.org)

This work describes the methodology for the analysis of terfenadine and the acid metabolite of terfenadine in plasma using high-performance liquid chromatography. (nih.gov)
With the combination of the ReagentDrop™ dispenser, Heater-Shaker, Gripper, and Nitrogen Dryer, VERSA automated solid phase extraction (SPE) systems act to produce contamination-free sample pre-processing and trace analyte enrichment before performing sample analysis by a chromatography-based platform (HPLC, GCMS, HPLC-MS, etc). (aurorabiomed.com)
The data generated in this study provides a solid foundation for subsequent work to further characterise (suspect screening) and quantify (target analysis) these substances. (springer.com)
This report aims to provide a comprehensive presentation of the global market for Solid Phase Extraction SPE Consumables, with both quantitative and qualitative analysis, to help readers develop business/growth strategies, assess the market competitive situation, analyze their position in the current marketplace, and make informed business decisions regarding Solid Phase Extraction SPE Consumables. (reportsnreports.com)
Chapter 2: Detailed analysis of Solid Phase Extraction SPE Consumables manufacturers competitive landscape, price, production and value market share, latest development plan, merger, and acquisition information, etc. (reportsnreports.com)

Dosage de la sélamectine dans le plasma de chien par chromatographie liquide haute performance avec une extraction en phase solide automatisée et une détection fluorimétrique. (vetres.org)
We included 995 individuals from the ZOE-PREDICT 1 study with plasma N-glycans measured by ultra-performance liquid chromatography at fasting and triglyceride, insulin, and glucose levels measured at fasting and following a mixed-meal challenge. (biomedcentral.com)
Quantitation of plasma total 15-series F(2)-isoprostanes by sequential solid phase and liquid-liquid extraction. (oregonstate.edu)

For this reason, a comparison of two solid phase extraction procedures, namely weak anionic exchange (WAX) and graphitized carbon black (GCB), is presented for the enrichment of phospholipids. (mass-analytica.com)

SPE is safer, less time consuming, and faster than liquid-liquid extraction. (coleparmer.com)
Extraction time of 60 min. (open.ac.uk)

The Manual solid phase extraction system is a negative pressure solid phase extraction device. (biolabscientific.com)

Selecting an organic modifier for reversed-phase chr. (chromatographytoday.com)

Utilizing mechanisms including normal phase, reversed phase, ion-exchange, and affinity chromatography and can be combined for mixed-mode chromatography applications. (coleparmer.com)

Reversed phase SPE separates analytes based on their polarity. (wikipedia.org)

According to the principle of similar solubility , polar solvents are chosen as extraction solvents . (bvsalud.org)

The chemistry considerations for the selection of stationary phases and the mobile phases are very similar in SPE and in chromatography. (wikipedia.org)
The Importance of Mobile Phase pH in Chromatogr. (chromatographytoday.com)

Analyses were performed by liquid chromatography-high resolution mass spectrometry (LC25 HRMS) and lipids were identified by Lipostar software. (mass-analytica.com)
Liquid chromatography-mass spectrometry (LC-MS) was used to identify components in fractionated CWAF. (nist.gov)

Many of the adsorbents/materials are the same as in Liquid column Chromatography methods, where similar materials are packed into long columns. (wikipedia.org)

Organisms2

Chemicals and Drugs4

Analytical, Diagnostic and Therapeutic Techniques and Equipment17

Phenomena and Processes3

Technology, Industry, Agriculture1

Health Care3

Automated Solid Phase Extraction3

Determination6

Stationary phase15

Separation5

Chromatographic1

Acetonitrile4

Procedure7

Elution1

Tandem7

HPLC-DAD1

Electrospray ionization-m1

Broccoli1

Detection1

Metabolites1

Analytes were determined1

QuEChERS1

Cartridge4

Serum1

Concentrations1

Simultaneous1

Vacuum1

Solvent2

Technique3

Capacity2

Temperature2

Analytical4

Fluid2

Aqueous2

Preparation4

Involves1

Sorbent2

Parameters1

Cartridges1

Sensitivity1

Analysis5

Plasma3

Weak1

Time2

System1

Organic1

Affinity1

Separates1

Polar1

Mobile2

Mass2

Column1