AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesDisciplines and OccupationsAnthropology, Education, Sociology and Social PhenomenaTechnology, Industry, AgricultureInformation ScienceHealth Care
Phase TransitionLipid BilayersChromatography, High Pressure LiquidPhosphatidylcholinesPhysicochemical PhenomenaChemistry, PhysicalMembrane Fluidity1,2-DipalmitoylphosphatidylcholineTemperatureUnilamellar LiposomesMembranes, ArtificialMembrane LipidsPolyethylene GlycolsPhysical PhenomenaCalorimetry, Differential ScanningChromatography, LiquidMembrane MicrodomainsPolymersIonic LiquidsWaterComplex MixturesDimyristoylphosphatidylcholineMolecular ConformationChemical FractionationBenzyl AlcoholDetergentsThermodynamicsbeta-KeratinsSolutionsPorosityModels, ChemicalPhysicsSphingomyelinsGelsPhosphatidylethanolaminesSurface PropertiesCell MembraneLiposomesPhospholipidsHydrophobic and Hydrophilic InteractionsGlycolsCholesterolNanostructuresLiquid CrystalsDiphenylhexatrieneX-Ray DiffractionSolubilitySurface-Active AgentsLauratesBiophysicsBiophysical PhenomenaMicroscopy, Atomic ForceCrystallizationFreeze FracturingThymolphthaleinColloidsMicroscopy, Electron, Scanning2-NaphthylaminePressureAnxiety, SeparationCattleSilicon DioxideHemoglobin CSpectrometry, FluorescenceBiocompatible MaterialsDiffusionDrug StabilityModels, BiologicalCell FractionationPhosphatidylglycerolsEmulsionsCrystallinsImmunomagnetic SeparationParticle SizeKineticsHydrogen-Ion ConcentrationMagnetic Resonance SpectroscopyComputer SimulationCell SeparationMicroscopy, FluorescenceFluorescent DyesMembrane ProteinsLipidsCompressive StrengthTransition TemperatureSpin LabelsElectrophoresis, Polyacrylamide GelMicrospheresBiopolymersSolventsLens, CrystallineChemistry, PharmaceuticalNanofibersScattering, RadiationMolecular StructureCalorimetryMaterials TestingPolyestersElectrophoresis, CapillaryMass Spectrometry
ChemistryStationary phaseHPLCReversed-phase liquid chromatographyReverse Phase Liquid ChromatographyMethodsTandem mass spectroProcedureMixturesPolymericSolventAnalytesUHPLCDetectionCompoundsCapillary electrophoresisLaboratoriesMicrofluidicElectrophoresisPreparativeQuantificationTechniquesRetentionMethodInstrumentationJournal of Separation ScienceDividing parametersParticlesSelectivityLaboratoryMechanismBehaviorDeterminationTemperatureMolecularMethodologiesGradientTheoreticalScientistMoleculesOccursScienceTechniqueColumnMassChromatography system
Chemistry15
  • The Analytical Scientist magazine named Dr. James Grinias, assistant professor, Department of Chemistry and Biochemistry at Rowan University, to its 40 under 40 "Power List" for 2018. (emich.edu)
  • He also studies the use of new technology to enhance educational outcomes in the undergraduate analytical chemistry curriculum. (emich.edu)
  • Grinias received his bachelor's degree in Chemistry from Eastern Michigan University in 2009 (where he won the Peet-Mayor Award that year as the top graduating senior) and his doctoral e in Analytical Chemistry from the University of North Carolina at Chapel Hill. (emich.edu)
  • High-performance liquid chromatography (HPLC) is an analytical chemistry technique used to separate compounds in a chemical mixture. (thermofisher.com)
  • Analytical Chemistry, 77, 7852-7861 (2005). (nist.gov)
  • Analytical Chemistry, 77, 7862-7871 (2005). (nist.gov)
  • It is a relatively 'new' form of analytical chemistry in that the first true use of modern chromatography is usually attributed to Russian botanist Mikhail Semyonovich Tsvet, who used columns of calcium carbonate for separating plant pigments during the first decade of the 20th century during his research of chlorophyll. (paduiblog.com)
  • Analytical Chemistry 82, 3692-3698 (2010). (edu.pl)
  • Wiczling P., Kaliszan, R.: Influence of pH on retention in linear organic modifier gradient RP HPLC Analytical Chemistry 80(20), 7855-7861 (2008). (edu.pl)
  • Their work established a form of chromatography called partition chemistry and was the basis for methods we now know as paper, gas, and liquid chromatography. (cannabissciencetech.com)
  • A continuous flow sequential multiple chemistry analyzer for clinical use is a modular analytical instrument intended to simultaneously perform multiple chemical procedures using the principles of automated continuous flow systems. (fda.gov)
  • A discrete photometric chemistry analyzer for clinical use is a device intended to duplicate manual analytical procedures by performing automatically various steps such as pipetting, preparing filtrates, heating, and measuring color intensity. (fda.gov)
  • The expertise of BGC in chemistry and analytics covers the whole analytical pipeline involving specific microsampling techniques, sample preparations, functional/structural analyses and bioinformatics. (helmholtz-munich.de)
  • Presentation of principles of green analytical chemistry and new research in this field. (lu.se)
  • During my previous research, I focused on combining experimental results, mainly from analytical chemistry, with novel theoretical models to try to understand the underlying physical mechanisms responsible for the observed phenomena. (lu.se)
Stationary phase27
  • These separations utilize the pressure-driven flow of a liquid mobile phase through a column packed with a stationary phase. (thermofisher.com)
  • Successful separation only occurs if the analytes have differing affinities for the stationary phase, so selecting the appropriate stationary phase for your compounds is crucial. (thermofisher.com)
  • The mixed components are placed at the top of the column of the stationary phase, which is generally a fine adsorbent solid such as silica. (europeanpharmaceuticalreview.com)
  • a component with a higher affinity for the stationary phase will migrate down the column slower than a component which has more affinity for the mobile phase. (europeanpharmaceuticalreview.com)
  • Representation of octadecyl stationary phase morphology showing an ordered state and a disordered state. (nist.gov)
  • Stationary phase order was found to increase with increased bonding density, increased chain length, and at decreased temperature. (nist.gov)
  • Molecular dynamics simulations of alkylsilane stationary-phase order and disorder. (nist.gov)
  • Liquid chromatography (LC) separates molecules in a liquid mobile phase by means of a solid stationary phase. (futuremarketinsights.com)
  • In is very basic nature, and grossly oversimplified, it involves passing a complex non-separated mixture presented to the machine in a 'mobile phase' expelled through a stationary phase. (paduiblog.com)
  • The stationary phase separates the analyte to be measured from other molecules of a different type present in the mixture based on differential partitioning between the mobile and stationary phases (affinity). (paduiblog.com)
  • This results in differential retention time on the stationary phase and thus making the separation. (paduiblog.com)
  • Gas chromatography (GC) is based on a partition equilibrium of analyte between a solid stationary phase (often a liquid silicone-based material) and a mobile gas (most often Helium). (paduiblog.com)
  • The stationary phase is adhered to the inside of a small-diameter glass tube (a capillary column) or a solid matrix inside a larger metal tube (a packed column). (paduiblog.com)
  • As in other chromatographic techniques, separation of the sample into its constituents occurs according to their affinity to the mobile and stationary phase. (iti.lk)
  • The HMDAs are separated via reversed-phase liquid chromatographic methods using a C,0 stationary phase and an acidic aqueous acetonitrile mobile phase. (erowid.org)
  • Chromatography is essentially a separation technique - the sample is carried along by a mobile phase (either gas or liquid) and the components of the sample mixture are separated due to their affinity for the stationary phase. (chromatographytoday.com)
  • During their work they had created columns packed with silica and water as a stationary phase and then used chloroform as a mobile phase to separate N-acetamino acids from protein hydrolysates. (cannabissciencetech.com)
  • Chromatography allows for the separation of a mixture of compounds by diluting the mixture into a mobile phase which then passes through or over a stationary phase to create separation by the interaction and affinity of individual compounds for one of the phases of the chromatography system ( Figure 1a ). (cannabissciencetech.com)
  • Stationary phase is a liquid or solid material that is fixed in a chromatography system. (cannabissciencetech.com)
  • The properties of the mobile phase and the stationary phase are opposite of each other and allow for the partitioning of analytes as the mobile phase and test mixture flow through or over the stationary phase ( Figure 1b ). (cannabissciencetech.com)
  • Gas chromatography (GC) uses a carrier gas, such as hydrogen or helium, to separate compounds based on their interaction with a stationary phase or based on their boiling points. (cannabissciencetech.com)
  • Mixtures are heated and vaporized in an injection port and are moved through or across a stationary phase to separate compounds before reaching a detector that provides data on concentration or identity of components. (cannabissciencetech.com)
  • In liquid chromatography (LC), compounds are separated by dissolving a sample into a liquid mobile phase which is then passed over a solid stationary phase. (cannabissciencetech.com)
  • UHPLC separation of R - and S -enantiomers of Besifloxacin hydrochloride on a new Chiralpak IE-3 column consisting of an immobilized amylose tris(3,5-dichlorophenylcarbamate) chiral stationary phase on 3 µm silica was studied. (springer.com)
  • The separation was accomplished under normal-phase conditions using a mobile phase composed of dichloromethane:isopropyl alcohol:trifluoroacetic acid:diethylamine (90:10:0.3:0.05 v/v/v/v) under isocratic mode of elution at 15 °C. The interactions between the analytes, additives, and the stationary phase were discussed and the most probable separation mechanism was proposed. (springer.com)
  • Thus these partially ionized molecules can interact with polar groups on the stationary phase of HPLC column, depending on the degree of end-capping of silanol groups. (mfa.org)
  • SFC is mainly decided by the stationary phase nature. (lu.se)
HPLC22
  • The analytical procedure involves enzymatic hydrolysis of glucuronidated/sulfated OH-PAH metabolites in urine, extraction by on-line solid phase extraction, and separation and quantification using isotope dilution high performance liquid chromatography-tandem mass spectrometry (on-line SPE-HPLC-MS/MS) (Wang et al. (cdc.gov)
  • Do you have questions about how high-performance liquid chromatography (HPLC) works? (thermofisher.com)
  • The most common form of liquid chromatography in use today is high-performance liquid chromatography (HPLC), which pumps the sample mixture through the column at high pressure. (europeanpharmaceuticalreview.com)
  • HPLC is the form of liquid chromatography that is generally used in the pharmaceutical industry, as it can provide the precise results that are required. (europeanpharmaceuticalreview.com)
  • Other forms of HPLC that are used in the pharmaceutical industry include reversed-phase, denaturing and immobilised enzyme reactor (IMER) HPLC. (europeanpharmaceuticalreview.com)
  • Liquid chromatography generally uses small particles packed and operating at comparatively high pressure, and is denoted as high performance liquid chromatography (HPLC). (futuremarketinsights.com)
  • Modern Liquid Chromatography-Mass Spectrometry (LC-MS) methods use HPLC instrumentation, basically for sample introduction. (futuremarketinsights.com)
  • HPLC is an instrumental analytical technique to separate compounds into their components. (iti.lk)
  • Unlike in normal liquid chromatography where the liquid passes through the column under gravity, HPLC uses high pressure to pass the sample mixture through the column packed with a solid adsorbent material. (iti.lk)
  • At ITI, we use three types of HPLC in our analytical work. (iti.lk)
  • A newly developed method uses a chiral selector part of HPLC columns available from multiple manufacturers and a fairly common mobile phase. (bvsalud.org)
  • Perisic-Janjic N., RKaliszan R., Wiczling P., Milosevic N., Uscumlic G., Banjac N., Reversed-phase TLC and HPLC retention data in correlation studies with in silico molecular descriptors and druglikeness properties of newly synthesized anticonvulsant succinimide derivatives, Molecular Pharmaceutics, 8, 555-563 (2011). (edu.pl)
  • Andrea F, Michaela V, Janka Mydlova JL, Jan K (2006) Interconversion of oxazepam enantiomers during HPLC separation. (springer.com)
  • The factor screening studies were performed using Box-Behnken design by three key components of the RP-HPLC method (mobile phase, pH and flow rate). (springeropen.com)
  • The current work intends to develop and optimize the RP-HPLC method by employing analytical QbD principles for the estimation of evogliptin tartrate pharmaceutical dosage form. (springeropen.com)
  • The present study reports the optimization and validation of a dissolution test for selegiline.HCl tablets using a new high-performance liquid chromatographic (HPLC) method. (shengsci.com)
  • In the present study two methods based on liquid chromatography with diode array detection (HPLC-DAD) coupled to an electrospray ionisation (ESI-MS) interface were developed for the determination of constituents in the tinctures (60%, v/v, DER 1:5) of turmeric (Curcuma longa L.) and Devil's claw (Harpagophytum procumb. (shengsci.com)
  • The reason for the tailing, and occasional changes in shape and elution time, is likely related to the use of dilute formic acid in the HPLC mobile phase. (mfa.org)
  • Extracts of plant material or of dyed silk or wool were analyzed by HPLC with photodiode array and mass spectrometric detection using an Agilent 1100 high performance liquid chromatography system consisting of an automatic injector, a gradient pump, a Hewlett-Packard series 1100 photodiode array detector, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. (mfa.org)
  • Oseltamivir and zanamivir are both well as a high-performance liquid chromatographic method approved by the Food and Drug Administration (FDA) for (HPLC), to measure the concentration of oseltamivir phos- use in controlling both infl uenza A and B viruses ( 1 ). (cdc.gov)
  • Optimisation of HPLC systems with different combinations of mobile and stationary phases. (lu.se)
  • 1 extraction was carried out using immunoaffinity column, separated by reversed-phase (C-18) high performance liquid chromatography (HPLC) , and quantified by fluorescence detector. (bvsalud.org)
Reversed-phase liquid chromatography4
  • Baczek T., Kaliszan R.: Predictions of peptides' retention times in reversed-phase liquid chromatography as a new supportive tool to improve protein identification in proteomics. (edu.pl)
  • Baczek T., Walijewski L., Kaliszan R.: pH gradient reversed-phase liquid chromatography as a fractionation tool for the separation of peptides. (edu.pl)
  • The primary amine (MDP-2-B) and the N-substituted derivatives of MDP-2-B are separated by reversed-phase liquid chromatography under acidic mobile-phase conditions. (erowid.org)
  • For my master's thesis, I studied the retention mechanism in reversed-phase liquid chromatography with integral equation theories (replika Ornstein-Zernike theory and Wertheim's multi-density theory) as the basis of the theoretical models. (lu.se)
Reverse Phase Liquid Chromatography2
Methods13
  • Our technical focus and capabilities include a range of extraction approaches, separation methods (solid and liquid phase), detection techniques, chromatographic techniques, spectroscopy (including accurate mass spectrometry, spectrofluorimetry, surface enhanced Raman spectroscopy and energy dispersive spectroscopy), and chemometric techniques for data handling and optimization. (bournemouth.ac.uk)
  • It can also be used in combination with other analytical methods to further elucidate the components of mixtures. (europeanpharmaceuticalreview.com)
  • Chemical isotope labelling LC-MS analysis methods have been developed for most other phytohormones, enhancing their LC separations and quantitative sensitivity. (unboundmedicine.com)
  • The combinations are categorized with more than one name and the names focus on many different aspects of liquid chromatography methods. (futuremarketinsights.com)
  • Methods: Urine (1 mL) from 6 exposed spray painters (n=24) was treated with sulfuric acid and heated for 24 h and then extracted with methanol:formic acid using solid-phase extraction with Phenomenex Strata-X reversed-phase columns. (cdc.gov)
  • We will dive deeper into the importance of understanding contaminant mass, as well as the various field and laboratory analytical methods that provide a foundation for those calculations. (cascade-env.com)
  • Liquid (or dissolved) phase mass removal is most commonly calculated using a selected laboratory analytical method (or methods) and flow rates through the above ground liquid treatment system. (cascade-env.com)
  • Gas chromatography is predominately used for analytical methods, rather than preparatory methods. (cannabissciencetech.com)
  • This normally requires the use of standards in carefully calibrating the analytical methods. (itrcweb.org)
  • Used methods comprises the quantification of selected small molecules of interest (long and short chain fatty acids (SCFA), bile acids, D/L amino acids, lipids, nucleotides, peptides, etc.) and the development of non-targeted standardized methods for the analysis of polar, mid- to non-polar metabolites utilizing hydrophilic interaction liquid chromatography (HILIC) and reversed phase (RP) chromatographic separation. (helmholtz-munich.de)
  • Analytical methods to quantify pesticide biomarkers in human population studies are critical for exposure assessment given the widespread use of pesticides for pest and weed control and their potential for affecting human health. (cdc.gov)
  • Critical discussions of the environmental sustainability of analytical methods and its relevance for society at large. (lu.se)
  • This completely new Third Edition of the Mark Encyclopedia of Polymer Science and Technology brings the state-of-the-art to the twenty-first century, with coverage of nanotechnology, new imaging and analytical techniques, new methods of controlled polymer architecture, biomimetics, and more. (lu.se)
Tandem mass spectro7
  • Starting with the current NHANES cycle, we used a different approach that relies on liquid chromatography-tandem mass spectrometry. (cdc.gov)
  • The extract was concentrated by vacuum centrifugation drying and analyzed with ultra-performance liquid chromatography/ tandem-mass spectrometry (UPLC-MS/MS). Analysis: UPLC-MS/MS analysis was performed with a nanoAcquity UPLC system (Waters Corp., Milford, MA) coupled to a TSQ Quantum Ultra triple-quadrupole MS (Thermo Fisher Scientific, Waltham, MA). (cdc.gov)
  • Title : Two-dimensional high performance liquid chromatography separation and tandem mass spectrometry detection of atrazine and its metabolic and hydrolysis products in urine☆ Personal Author(s) : Kuklenyik, Zsuzsanna;Panuwet, Parinya;Jayatilaka, Nayana K.;Pirkle, James L.;Calafat, Antonia M. (cdc.gov)
  • Recently we proposed an isocratic enantioselective high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the separation and quantitative determination of dextro- (DXM) and levo-methorphan (LVM) and their pharmacologically relevant metabolites, dextrorphan and levorphanol, respectively, in human blood samples. (bvsalud.org)
  • Arnold DR, Granvil CP, Ward KW, Proksch JW (2008) Quantitative determination of besifloxacin, a novel fluoroquinolone antimicrobial agent, in human tears by liquid chromatography-tandem mass spectrometry. (springer.com)
  • A sensitive, rapid and selective liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed and validated for the simultaneous determination of baicalin, baicalein, wogonin, berberine, palmatine and jatrorrhizine in scutellaria-coptis herb couple in rat plasma. (shengsci.com)
  • The method is based on enzymatic hydrolysis of conjugated urinary metabolites, extraction and pre-concentration of the deconjugated metabolites using automated online solid-phase extraction, and separation and quantification using liquid chromatography-isotope dilution tandem mass spectrometry. (cdc.gov)
Procedure5
  • Liquid chromatography procedure can be directed in various combination of possible mobile phases and stationary phases. (futuremarketinsights.com)
  • The distillation step which can represent up to 80% of the total analytical procedure is no longer required. (iti.lk)
  • Over the subsequent decades this procedure was expanded as a separation technique by the Nobel Prize winning work of Archer J.P. Martin and Richard L.M. Synge in the 1940s and 1950s. (cannabissciencetech.com)
  • By carefully understanding the step-by-step procedure for analytical QbD-based optimization parameters, such as analytical target profile and critical quality attributes (CQAs), was assessed. (springeropen.com)
  • Blanks - samples containing extremely low concentrations of a contaminant , which are used to assess contamination from laboratory equipment and other steps in an analytical procedure. (cdc.gov)
Mixtures2
Polymeric3
  • A proposed mechanism for this unique ability combines the shape selectivity of polymeric phases, previously recognized for planar polycyclic aromatic hydro¬ carbons, and the different 'molecular footprints' of the isomers. (canada.ca)
  • These models offer insight into selectivity trends observed in liquid chromatography with C18 and C30 stationary phases, prepared by monomeric or polymeric synthetic approaches, and operated at different column temperatures. (nist.gov)
  • His work has provided a new molecular understanding of non-equilibrium polymer dynamics, bridging the gap between molecular behavior and bulk properties in polymeric liquids. (rheology.org)
Solvent4
  • UHPLC separations enable higher peak resolution, improved analyte sensitivity, shorter analysis times and less solvent consumption. (thermofisher.com)
  • Solvent extraction and separation tech. (slideshare.net)
  • Similar a Solvent extraction and separation tech. (slideshare.net)
  • Mobile phase is a solvent or gas that flows through a chromatography system and is also referred to as an eluant . (cannabissciencetech.com)
Analytes3
  • Reversed-phase separation was carried out using an Atlantis dC18 analytical column, 3 um, 100 um x 100 mm (Waters Corp). Analytes were ionized by nano-electrospray ionization in the positive-ion mode and detected using selected reaction monitoring. (cdc.gov)
  • Wiczling P., Kaliszan R,: pH Gradient as a tool for the separation of ionizable analytes in reversed-phase high-performance chromatography. (edu.pl)
  • Wiczling P., Waszczuk-Jankowska M., Markuszewski M.J., Kaliszan R.: The application of gradient reversed-phase high-performance liquid chromatography to the pKa and log kw determination of polyprotic analytes. (edu.pl)
UHPLC1
Detection9
  • BU ASRG is the hub of analytical science at the university for both research and commercial projects, providing state-of-the-art analytical instrumentation and technical expertise for sample extraction, separation, detection and characterisation. (bournemouth.ac.uk)
  • However, some components may have weak UV chromophores if UV detection is being used or be completely retained on the liquid chromatography column. (europeanpharmaceuticalreview.com)
  • This new analytical method has been developed by the IFRA Analytical Working Group to identify and to quantify the 57 fragrance substances and their relevant isomers at a concentration higher than 0.002% (20 mg/kg) in ready to inject fragrance materials and oils using Gas Chromatography with FID (GC-FID) or with Mass Spectrometry (GC-MS) detection. (sigmaaldrich.com)
  • When we started AES back in 2010, we knew that we wanted to build a company that would be dedicated to providing the life science industry the most reliable, highest performance and highest throughput whole column imaging detection capillary electrophoresis system for protein separation, quantification, and characterization. (abbabio.com)
  • In this work, we combine a monolith and µPAD to form a novel surface-modified microfluidic-based analytical device (sPAD) with better biomolecular immobilization efficiency and stability for TB detection. (ntu.edu.tw)
  • The optimal chromatographic separation was achieved having water C18 column (250 mm × 4.6 mm, 5 μ) and using mobile phase as a methanol and phosphate buffer (pH 4.5) 60:40% v/v with a flow rate 1.0 ml/min and UV detection at 267 nm. (springeropen.com)
  • Rapid separation of the analyte from sample matrix was achieved in less than 60s using a Cromolith RP-18e monolithic column using UV detection at 220 nm. (shengsci.com)
  • The reverse-phase chromatographic assay uses an alkaline mobile phase with UV detection. (cdc.gov)
  • Mass spectrometric detection for quantitative and qualitative analysis connected to both gas and liquid chromatography. (lu.se)
Compounds4
  • It is not uncommon to detect significant vapor phase concentrations of less volatile compounds with higher boiling points, such as semi-volatile organic compounds (SVOCs), polychlorinated biphenyls (PCBs), pesticides, or dioxin/furans, when present at the site. (cascade-env.com)
  • The analytical properties of these compounds are compared with the structurally similar 3,4-methylenedioxyamphetamine (MDA) derivatives, a popular series of drugs of abuse. (erowid.org)
  • The compounds are not completely resolved by gas chromatography on an HP-1 phase, and the separation is complicated by extensive thermal degradation of the N-hydroxy derivative (MDP-2-OHB). (erowid.org)
  • This new development worked with the affinity of the target compounds with the different phases allowing for separation and collection of individual compounds. (cannabissciencetech.com)
Capillary electrophoresis1
Laboratories4
  • Please see our facilities page to explore the range of specialist analytical instrumentation in our laboratories. (bournemouth.ac.uk)
  • Increasing adoption of liquid chromatography-based systems and instruments in research laboratories. (futuremarketinsights.com)
  • The proposed analytical method is relatively simple, accurate and fast and can be adopted for routine use in forensic and clinical toxicology laboratories. (bvsalud.org)
  • Since the initial discoveries of the early to mid-20th century the science of chromatography has seen rapid growth and expansion into an important and fundamental tool of many analytical laboratories. (cannabissciencetech.com)
Microfluidic2
  • Grinias spent the past several years researching the fundamentals of liquid-phase separations in capillary and microfluidic columns as well as applications in neuroscience, molecular physiology, and pharmaceutical fields. (emich.edu)
  • Microfluidic paper-based analytical devices (µPADs) are a typical type of POC device. (ntu.edu.tw)
Electrophoresis2
Preparative2
Quantification1
  • This is achieved through the separation, quantification and identification of components in a mixture and can be used to reveal the identity of a drug and monitor the progress of a therapy on a disease. (europeanpharmaceuticalreview.com)
Techniques10
  • Our group offer an array of analytical techniques and services to industrial clients including contract research, routine analysis and training courses, and can provide timely answers to difficult analytical problems. (bournemouth.ac.uk)
  • To guarantee that goods pass certain standards, researchers, manufacturers and developers use various technical equipment and analytical techniques , including liquid chromatography, during the development process. (europeanpharmaceuticalreview.com)
  • There is increasing demand from both pharmaceutical companies and regulatory bodies for increasingly sensitive analytical techniques that can provide the highest possible assurance that a product is of acceptable quality and will provide the promised efficacy safely. (pittcon.org)
  • Consequently, analytical techniques are needed to characterize the product rapidly with a high level of accuracy, sensitivity and resolution. (pittcon.org)
  • We have investigated the conformational phase behavior of tethered chain systems using temperature expanded Monte Carlo techniques at thousands of state points, covering densities from the isolated chains to the thin film limit, and a broad range of temperatures. (nist.gov)
  • During the pandemic, high-performance, liquid chromatography techniques became a reliable component for developing life-saving vaccines and other disease-detecting systems. (futuremarketinsights.com)
  • Analytical techniques, for example chromatography and mass spectrometry, along with various tools such as cloning, CRISPR/Cas, single-use equipment, machine learning [and] use of advanced sensors and automation have helped in the advancement of biopharma research," said Dr. Johannes Buyel , head of the department of Bioprocess Engineering at the Fraunhofer IME and associate professor at the RWTH Aachen University. (technologynetworks.com)
  • Along with spectroscopy, chromatography is one of the major analytical techniques at the disposal of any chemical or biochemical researcher. (chromatographytoday.com)
  • From simple separations to detailed wavelength analysis, we examine the important concepts and techniques needed to make liquid chromatography work its best in the laboratory. (cannabissciencetech.com)
  • This module introduces the theoretical basis and current and future developments in analytical separation techniques. (hud.ac.uk)
Retention7
  • Benzylation alters the hydrophilicity of CKs and their glycoconjugates, improving their retention and separation on RPLC. (unboundmedicine.com)
  • The GC itself is a qualitative analytical method whereby if it alone is used, it can only tell that the characteristic is present by the retention time as compared against a known At best, using only a GC, you can tell that the suspect analyte is consistent with the characteristics of the known. (paduiblog.com)
  • Molikova M., Markuszewski M.J., Kaliszan R., Jandera P.: Chromatographic behaviour of ionic liquid cations in view of quantitative structure-retention relationship. (edu.pl)
  • Wiczling P., Kaliszan R,: Retention time and peak width in the combined pH/organic modifier gradient high performance liquid chromatography. (edu.pl)
  • Michel M., Baczek T., Studzinska S., Bodzioch K., Jonsson T., Kaliszan R., Buszewski B.: Comparative evaluation of high-performance liquid chromatography stationary phases used for the separation of peptides in terms of quantitative structure-retention relationships. (edu.pl)
  • The selection and effects of mobile phase solvents, additives, organic modifiers, and column temperature on retention, separation, and resolution were investigated and optimized. (springer.com)
  • The Box-Behnken experimental design describes the interrelationship of mobile phase, pH and flow rate at three different levels, and responses of retention time and tailing factor were observed with response surface plot and statistical data. (springeropen.com)
Method12
  • Here, we report a new chemical isotope labelling LC-MS analytical method for one-pot derivatization of CK bases and their glycoconjugates, based on differential benzylation labelling of the adenine skeleton of CKs with benzyl bromide and its deuterium isotope-labelled reagent. (unboundmedicine.com)
  • This method was based on the polysaccharide-based chiral column Lux AMP, a specialty column characterized with high stability in mobile phases of pH 11.0 and above. (bvsalud.org)
  • The use of a single-source column is a limitation for any analytical method. (bvsalud.org)
  • Therefore, the major goal of the present study was to develop an enantioselective method for the differentiation of dextro- and levo-methorphan, as well as their metabolites dextrorphan and levorphanol, using Lux Cellulose-3 as alternative chiral column with methanol containing 0.1 % diethylamine mobile phase. (bvsalud.org)
  • If the boiling point difference between two elements is less than 50°C, this method will not work effectively since both liquids will evaporate together losing the advantage of obtaining separate vapor streams. (physics-network.org)
  • Implementation of QbD principle for analytical method development has been practised quite popular for attaining the high robustness and enhance method performance. (springeropen.com)
  • Development and validation of a liquid chromatographic method for the determination of ascorbic acid, dehydroascorbic acid and acetaminophen in pharmaceuticals. (shengsci.com)
  • A reversed-phase ion pair liquid chromatographic method (RP-LC) for the determination of dehydroascorbic acid (DHA) and ascorbic acid (AA) and also acetaminophen, which is combined in pharmaceuticals, is proposed and validated. (shengsci.com)
  • Accuracy - the ability of an analytical method to measure the true concentration of a contaminant ( see bias, precision ). (cdc.gov)
  • Analytical method - a laboratory test used to detect the amount of a contaminant . (cdc.gov)
  • Precision refers to the ability of an analytical method to reproduce the same result upon repeated trials. (cdc.gov)
  • Furthermore, the course aims to enable students to develop their ability to independently select and optimise a suitable separation technique/method and to present a coherent holistic view of the subject. (lu.se)
Instrumentation1
  • The BU Analytical Science Research Group (ASRG) offers a range of specialist analytical instrumentation and is a hub of analytical science at BU for both research and commercial projects. (bournemouth.ac.uk)
Journal of Separation Science1
  • Journal of Separation Science 33, 1546-1557 (2010). (edu.pl)
Dividing parameters1
  • It's especially useful for the separation of chemicals like petrochemicals that have no distinctive dividing parameters and boil within tight relative boiling temperature ranges. (physics-network.org)
Particles1
  • Aerosol - a suspension of solid and/or liquid particles in a gas (like air). (cdc.gov)
Selectivity2
  • In this article we will discuss the introduction of new certified reference materials and their analysis by GC using an ionic liquid column as an alternative with unique selectivity and stability as compared to traditional wax/PEG phases. (sigmaaldrich.com)
  • Additional developments include new dopants for enhanced electrospray ionization, quantitative structure-selectivity relationship (QSSR) modeling of chromatographic behavior of metabolites in RP and HILIC separations, miniaturization designs of the technologies for minimal sample volumes and in-silico analysis of MS/MS fragmentation patterns. (helmholtz-munich.de)
Laboratory2
  • Commonly made in the laboratory by solid-phase chemical synthesis, these small bits of nucleic acids can be manufactured as single-stranded molecules with any user-specified sequence, and so are vital for artificial gene synthesis, polymerase chain reaction (PCR), DNA sequencing, molecular cloning and as molecular probes. (wikipedia.org)
  • To confirm the field instruments' readings, a laboratory analytical sampling program is always implemented. (cascade-env.com)
Mechanism1
Behavior5
  • Predicting how the conformational phase behavior of tethered chain molecules depends on external variables is challenging. (nist.gov)
  • To determine how variables such as temperature, chain density, chain length, surface interactions, etc., impact the conformational phase behavior of tethered chains. (nist.gov)
  • To date, we have investigated the impact of surface interactions, chain stiffness, and tethering geometry on conformational phase behavior. (nist.gov)
  • We have demonstrated that simulation may be used as an effective guide to understanding the conformational phase behavior of tethered chain systems. (nist.gov)
  • Hello, A couple of things I'd like some clearing up on regarding the behavior of (residual) silanols on chromatographic silica phases in different environments. (chromforum.org)
Determination1
  • Wang Z, Wang S, Zhu F, Chen Z, Yu L, Zeng S (2012) Determination of enantiomeric impurity in besifloxacin hydrochloride by chiral high-performance liquid chromatography with precolumn derivatization. (springer.com)
Temperature1
  • Another advantage claimed precision heating control because temperature maintenance takes place at low enough atmospheric pressure levels to permit separations focused around precise heat conduction rather than inefficient forced boiling. (physics-network.org)
Molecular3
  • groups bonded to the fullerene surface appear to disrupt adsorptive interactions between the fullerene molecular footprints and the stationary phases. (canada.ca)
  • In a separate effort, alkyl stationary phases used in liquid chromatography have been modeled by molecular dynamic simulations, under chromatographically relevant conditions. (nist.gov)
  • Shear-induced undulation of smectic-A: Molecular dynamics simulations vs. analytical theory. (mpg.de)
Methodologies2
  • It is important for pharmaceutical companies to have the necessary analytical methodologies in place early in the development process so potential impurities are identified early on and are not a surprise at the time approval is sought. (pittcon.org)
  • Success in achieving the goal of developing new and safe medicines faster is highly dependent on the development of appropriate analytical methodologies. (pittcon.org)
Gradient3
  • As the ACN fraction increases during a gradient separation, I would assume the medium effect of ACN influences the dissociation of FA causing the equilibrium to shift towards the neutral acid. (chromforum.org)
  • Baczek T., Bodzioch K., Michalska E., Kaliszan R.: Predictions of reversed-phase gradient elution LC separations supported by QSRR. (edu.pl)
  • It is also defined as: The process of transfer of fluids (vapors or liquid) through a geologic formation in response to a pressure gradient that may be caused by changes in barometric pressure, water table levels, wind fluctuations, or infiltration. (itrcweb.org)
Theoretical2
  • Lectures and exercises (7.5 credits): In-depth theoretical treatment of chromatographic separation and underlying distribution and adsorption equilibria. (lu.se)
  • Whereas theoretical predictions of macromolec- use of nonadsorbing polymers, like polyethylene glycol, for ular crowding effects abound, there are remarkably few protein separation (Albertsson, 1986) and crystallization experimental studies of this universal phenomenon. (lu.se)
Scientist1
  • The Analytical Scientist publication celebrates technology and innovations shaping measurement science. (emich.edu)
Molecules1
  • Adsorption is the adhesion of molecules of gas, liquid, or dissolved solids to a surface. (itrcweb.org)
Occurs1
Science5
  • The Analytical Science Research Group (ASRG) at Bournemouth University provides a focus for analytical research in the Department of Life and Environmental Sciences , and a platform for collaborative work across the university and with industry. (bournemouth.ac.uk)
  • We pride ourselves on our ability to solve problems through analytical science. (bournemouth.ac.uk)
  • What you are doing here is technically speaking separation science. (paduiblog.com)
  • You will be provided with a comprehensive overview of the most recent technological developments and applications in Pharmaceutical and Analytical Science. (hud.ac.uk)
  • Separation science is our expertise over the last decades. (helmholtz-munich.de)
Technique7
  • Liquid chromatography is an analytical technique utilised widely in the pharmaceutical industry. (europeanpharmaceuticalreview.com)
  • Liquid chromatography is an analytical technique that is used to separate a certain sample into its individual components. (europeanpharmaceuticalreview.com)
  • It is a separation technique in which the mobile phase is a gas. (paduiblog.com)
  • It is also an analytical technique used for elemental analysis. (iti.lk)
  • The process of separating a compound into its components is known as the separation technique. (physics-network.org)
  • Simple distillation is a separation technique that involves heating a liquid mixture until it boils. (physics-network.org)
  • The high separation power and short analysis time obtained demonstrate for the first time that supercritical fluid chromatography is a fast and reliable technique for the analysis of lignin-derived phenols in complex environmental samples. (lu.se)
Column6
  • they will have varying levels of affinity for the mobile phase, resulting in migration through the column at different speeds. (europeanpharmaceuticalreview.com)
  • Irrespective of the interactions that are being brought, liquid column chromatography is taken up in six steps: Column equilibration, Sample loading, Washing, Elution, Final column washing and Column regeneration. (futuremarketinsights.com)
  • Once the sample is injected into the instrument it enters to the separation column with the carrier gas. (iti.lk)
  • B) what if the water-phase on such a silica column was gradually displaced with acetonitrile (ACN)? (chromforum.org)
  • In this column, we will examine an important branch of chromatography essential to the cannabis industry-liquid chromatography. (cannabissciencetech.com)
  • As the liquid progressed through the column over several hours, bands of color were observed that were later identified as chlorophyll a, chlorophyll b, xanthine, and carotene. (cannabissciencetech.com)
Mass8
  • The separation of fullerenes and their derivatives on four commercially available reversed-phase columns was investigated using liquid chroma¬tography/mass spectrometry with atmospheric pressure chemical ionization. (canada.ca)
  • LCMS-MS is usually a high-performance liquid chromatograph combined with two mass spectrometers. (iti.lk)
  • Mass removal in the vapor phase is the most common phase in which mass removal is calculated, and is completed for all thermal remedies. (cascade-env.com)
  • These samples are typically collected after free-phase product separation to minimize overlap in mass calculations. (cascade-env.com)
  • The Department also has an associated flavour analytical suite, including proton transfer reaction mass spectrometry ( PTR-MS ) and gas chromatography ( GC ) suite. (otago.ac.nz)
  • As will be dis- cussed in more detail later, the chemical mass transport across gas, liquid/ tissue, and blood phases of the airways, as determined by these properties, was conceptualized in terms of the overall mass-transport coefficient ( K g) as a measure of the conductivity of a chemical across the three phases. (cdc.gov)
  • In- versely, when expressed as its reciprocal (1/ K g), K g is also a measure of the overall mass-transport resistance of the radial transport of the absorbing gas from the gas phase, through the liquid/tissue, and into blood. (cdc.gov)
  • Similarly, the overall mass-transport resistance to the radial transport of the gas (1/ K g) may be viewed as the summation of the resistance through each phase (i.e., analogous to electrical resistance summations) as shown in Figure 2. (cdc.gov)
Chromatography system1
  • The results show that comprehensive two-dimensional liquid chromatography system is of great importance and high value in the separation of complex TCM. (edu.hk)