Measuring Mouse Arachidonate 5-lipoxygenaInhibitorInhibitorsInhibitionPathwayALOX5AntibodyArachidonic acid metabEnzymesMetabolitesHumansAllene oxide1.13.11.12InflammationCyclooxygenaseMetabolismLinoleic acidBarleyLeukotrieneEnzymeMembraneTherapeuticHydroperoxyEicosanoidsHydroperoxideProteinsInhibitELISASequencePlantRegulationActivitySpecificActivationRoleHumanCellsDescriptionFemale
Measuring Mouse Arachidonate 5-lipoxygena1
- Description: Quantitative sandwich ELISA for measuring Mouse Arachidonate 5-lipoxygenase (ALOX5) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. (glideruniversity.org)
Inhibitor4
- 12-Lipoxygenase Inhibitor Improves Functions of Cytokine-Treated Human" by Kaiwen Ma, An Xiao et al. (lvhn.org)
- 12-Lipoxygenase Inhibitor Improves Functions of Cytokine-Treated Human Islets and Type 2 Diabetic Islets. (lvhn.org)
- A phase II study of the 5-lipoxygenase inhibitor, CV6504, in advanced pancreatic cancer: correlation of clinical data with pharmacokinetic and pharmacodynamic endpoints. (ox.ac.uk)
- In intact tissues this lipoxygenase inhibitor converted the arachidonate-induced relaxation to a concentration-dependent contraction. (cdc.gov)
Inhibitors2
- Maintaining the correct balance between prostaglandins and leukotrienes is essential for continuing good health, but both classes of mediators also play an important role in the pathogenesis of several diseases.Recently, a new class of anti-inflammatory drugs, the lipoxygenase (LOX)/COX inhibitors, has been developed as a means of simultaneously inhibiting the synthesis of prostaglandins, thromboxanes and leukotrienes. (unipv.it)
- Included under this category are inhibitors that are specific for lipoxygenase subtypes and act to reduce the production of LEUKOTRIENES. (bvsalud.org)
Inhibition1
- inhibition of 5-lipoxygenase (5-LO), which is expected to be involved in DHEA- and NALA-degradation pathway, also partially blocked the ability of DHEA and NALA to inhibit cell proliferation and phosphorylated Akt. (biomedcentral.com)
Pathway10
- The lipoxygenase pathway is responsible for the production of oxylipins, which are important compounds for plant defence responses. (unboundmedicine.com)
- Current evidence on the subcellular localization of the lipoxygenase pathway is conflicting, and the regulation of metabolic channelling between the two branches of the pathway is largely unknown. (unboundmedicine.com)
- Wound-induced accumulation of the jasmonic acid biosynthetic enzymes may thus commit the lipoxygenase pathway to jasmonic acid production in damaged plants. (unboundmedicine.com)
- It is shown that all enzymes of the lipoxygenase pathway differentially localize within chloroplasts, and are largely found associated to thylakoid membranes. (unboundmedicine.com)
- It is suggested that, in addition to their differential expression pattern, this segregation underlies the regulation of metabolic fluxes through the alternative branches of the lipoxygenase pathway. (unboundmedicine.com)
- Enteroaggregative Escherichia coli promotes transepithelial migration of neutrophils through a conserved 12-lipoxygenase pathway. (umassmed.edu)
- Breast cancer cell-derived EMMPRIN stimulates fibroblast MMP2 release through a phospholipase A(2) and 5-lipoxygenase catalyzed pathway. (ox.ac.uk)
- CONTEXT: The 12-lipoxygenase (12-LO) pathway produces proinflammatory metabolites, and its activation is implicated in islet inflammation associated with type 1 and type 2 diabetes (T2D). (lvhn.org)
- Eicosanoids are formed from arachidonic acid (AA) through different enzymatic pathways, including the cyclooxygenase (COX) pathway, synthesizing prostanoids and the 5-lipoxygenase (5-LOX) pathway, generating leukotrienes (LTs) 8 . (nature.com)
- Leukotrienes belong to a family of biologically active conjugated trienes that are formed from arachidonic acid via the 5-lipoxygenase pathway and are important mediators of inflammatory reactions. (lu.se)
ALOX55
- The 6 human lipoxygenases along with some of the major products that they make, as well as some of their associations with genetic diseases, are as follows: Arachidonate 5-lipoxygenase (ALOX5) (EC 1.13.11.34InterPro: IPR001885), also termed 5-lipoxygenase, 5-LOX, and 5-LO. (wikipedia.org)
- Description: Quantitativesandwich ELISA kit for measuring Rat Arachidonate 5-lipoxygenase (Alox5) in samples from serum, plasma, cell culture supernates, tissue homogenates. (glideruniversity.org)
- Description: The ALOX5 gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. (glideruniversity.org)
- Description: Quantitativesandwich ELISA kit for measuring Human Arachidonate 5-lipoxygenase (ALOX5/LOG5) in samples from serum, plasma, cell culture supernates, tissue homogenates. (glideruniversity.org)
- In the present study, we examined the molecular mechanism by which multi-walled CNTs (MWCNTs) induce M1 polarization in vitro, with focus on induction of arachidonate 5-lipoxygenase (ALOX5), which is required to produce proinflammatory LMs, such as leukotriene B4 (LTB4). (cdc.gov)
Antibody2
- This antibody recognizes with high specificity a Vitis vinifera chloroplastic lipoxygenase expressed in the berries along development (by western blotting). (agrisera.com)
- 5 Lipoxygenase Monoclonal antibody Storage Temperature: Store at -20°C. Stable for one year after shipment. (ols-bio.de)
Arachidonic acid metab2
- Cyclooxygenase (COX)-2 and 5-lipoxygenase (5-LOX) are key enzymes involved in arachidonic acid metabolism. (unifi.it)
- The contraction in denuded trachea, and trachea in the presence of indomethacin, may be mediated by lipoxygenase products of arachidonic acid metabolism, i.e. peptidoleukotrienes. (cdc.gov)
Enzymes3
- Based on detailed analyses of 15-lipoxygenase 1 and stabilized 5-lipoxygenase, lipoxygenase structures consist of a 15 kilodalton N-terminal beta barrel domain, a small (e.g. ~0.6 kilodalton) linker inter-domain (see Protein domain § Domains and protein flexibility), and a relatively large C-terminal catalytic domain which contains the non-heme iron critical for the enzymes' catalytic activity. (wikipedia.org)
- Lipoxygenase enzymes are widely distributed in both the plants and animals. (pbkom.eu)
- Lipoxygenases form a heterogeneous family of lipid peroxidizing enzymes, which have been implicated in the synthesis of inflammatory mediators, in cell development and in the pathogenesis of various diseases with major health and political relevance (atherosclerosis, osteoporosis). (univaq.it)
Metabolites2
- In general, various phospholipase A2s and diacylglycerol lipases are activated during cell stimulation, proceed to release these fatty acids from their storage sites, and thereby are key regulators in the formation of lipoxygenase-dependent metabolites. (wikipedia.org)
- moreover, these effects were mediated by the lipoxygenase (LOX) and cytochrome P450 (CYP450) EPA/DHA metabolites, 5-hydroxyeicosapentaenoic acid (HEPE), 4-hydroxydocosahexaenoic acid (HDHA), 18-HEPE, 20-HDHA, 17(18)-epoxyeicosatetraenoic acid (EpETE) and 19(20)-epoxydocosapentaenoic acid (EpDPA), detected here for the first time in human hippocampal neurones using mass spectrometry lipidomics of the supernatant. (nih.gov)
Humans1
- Exceptions to this rule include the 12R-lipoxygenases of humans and other mammals (see below). (wikipedia.org)
Allene oxide1
- It is shown here that while a 13-lipoxygenase (LOX H3), allene oxide synthase and allene oxide cyclase proteins accumulate upon wounding in potato, a second 13-lipoxygenase (LOX H1) and hydroperoxide lyase are present at constant levels in both non-wounded and wounded tissues. (unboundmedicine.com)
1.13.11.121
- Sequence data is available for the following lipoxygenases: Plants express a variety of cytosolic lipoxygenases (EC 1.13.11.12InterPro: IPR001246) as well as what seems to be a chloroplast isozyme. (wikipedia.org)
Inflammation3
- Systemic disease during Streptococcus pneumoniae acute lung infection requires 12-lipoxygenase-dependent inflammation. (umassmed.edu)
- Leukotrienes derived from 5-lipoxygenase (5-LOX) cause inflammation and are thus involved in the pathobiology of stroke injury. (biomedcentral.com)
- Lipoxygenases (LOX) contribute to vascular disease and inflammation through generation of bioactive lipids, including 12-hydro(pero xyeicosatetraenoic acid (12-H(P)ETE). (reading.ac.uk)
Cyclooxygenase1
- The present study aimed to ivnestigate the fole of 5-lipoxygenase (5-LOX)- and cyclooxygenase-2 (COX-2)- dependent enzymatic pathways in the pathogenesis of porcine parasitic bronchopneumonia caused by Metastrongylus spp. (uniba.it)
Metabolism2
- In addition, cells, when so activated, may transfer their released polyunsaturated fatty acids to adjacent or nearby cells which then metabolize them through their lipoxygenase pathways in a process termed transcellular metabolism or transcellular biosynthesis. (wikipedia.org)
- In mammals a number of lipoxygenases isozymes are involved in the metabolism of eicosanoids (such as prostaglandins, leukotrienes and nonclassic eicosanoids). (wikipedia.org)
Linoleic acid1
- Linoleic acid was used as the substrate from optimizing the assay of barley lipoxygenase in the cultivar Larker. (cerealsgrains.org)
Barley3
- Physicochemical Characterization of Barley-Lipoxygenase. (cerealsgrains.org)
- Barley lipoxygenase was not inhibited by iron binding compounds. (cerealsgrains.org)
- A full length cDNA sequence for a barley grain lipoxygenase was obtained. (tno.nl)
Leukotriene2
- It was shown that in vitro 11-keto-boswellic acids inhibit 5-lipoxygenase (5-LO, EC 1.13.11.34), the key enzyme in leukotriene biosynthesis, which may account for their anti-inflammatory effectiveness. (nih.gov)
- 5-Lipoxygenase, Human, Recombinant, S. fruigiperda 5-Lipoxygenase, Human, Recombinant, S. frugiperda E.C 1.13.11.34, catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A4. (sigmaaldrich.com)
Enzyme5
- A compound or agent that combines with lipoxygenase and thereby prevents its substrate-enzyme combination with arachidonic acid and the formation of the icosanoid products hydroxyicosatetraenoic acid and various leukotrienes. (foodb.ca)
- Also described are properties of biochemical and molecular reactions, including lipoxygenases which overlap with the enzyme in plant cells. (pbkom.eu)
- The crystal structures of various lipoxygenase-isoforms have been reported, and X-ray coordinates for enzyme-ligand complexes are also available. (univaq.it)
- Taken together, our results indicate that the rabbit 12/15-lipoxygenase is more susceptible to temperature-induced structural alterations than the soybean enzyme. (univaq.it)
- thus, we sought to ascertain whether a prototypical enzyme like soybean (Glycine max) 15-lipoxygenase-1 is able to oxygenate endocannabinoids embedded in synthetic vesicles and how these substances could affect the binding ability of the enzyme to different lipid bilayers. (unite.it)
Membrane3
- 2015). The onset of grapevine berry ripening is characterized by ROS accumulation and lipoxygenase-mediated membrane peroxidation in the skin. (agrisera.com)
- Applying fluorescence resonance energy transfer we also tested the membrane binding properties of the two lipoxygenase-isoforms, and compared their binding parameters. (univaq.it)
- and that (iii) 15-lipoxygenase-1 oxidizes embedded endocannabinoids and thus reduces fluidity and local hydration of membrane lipids. (unite.it)
Therapeutic1
- These results suggest that the production of soluble EMMPRIN, phospholipase A(2) and 5-lipoxygenase activities are sites for potential therapeutic intervention. (ox.ac.uk)
Hydroperoxy2
- Most lipoxygenases catalyze the formation of initially formed hydroperoxy products that have S chirality. (wikipedia.org)
- In particular, lipoxygenases can generate hydroperoxy derivatives of endocannabinoids, endowed with distinct activities within cells. (unite.it)
Eicosanoids1
- MDM from convalescent SARS-CoV-2-infected individuals showed a downregulation of pro-resolving factors and an increased production of pro-inflammatory eicosanoids, particularly 5-lipoxygenase-derived leukotrienes. (nature.com)
Hydroperoxide1
- Plant lipoxygenase in conjunction with hydroperoxide lyases are responsible for many fragrances and other signalling compounds. (wikipedia.org)
Proteins1
- while the third domain of terrestrial life, the archaea, possesses proteins with a slight (~20%) amino acid sequence similarity to lipoxygenases, these proteins lack iron-binding residues and therefore are not projected to possess lipoxygenase activity. (wikipedia.org)
Inhibit1
- Compounds that bind to and inhibit that enzymatic activity of LIPOXYGENASES. (bvsalud.org)
ELISA1
- Description: A competitive ELISA for quantitative measurement of Canine Arachidonate 15 lipoxygenase (ALOX15/LOG15) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (myelisakit.com)
Sequence1
- Its amino acid sequence shows a high homology with that of other plant lipoxygenases identified to date. (tno.nl)
Plant4
- The article discusses the structure and function of plant lipoxygenases. (pbkom.eu)
- In addition to discussing the physiological role of lipoxygenase in plants, plant lipoxygenases described role in food technology and industry. (pbkom.eu)
- Structural properties of plant and mammalian lipoxygenases. (univaq.it)
- Although the 3D-structures of plant and animal lipoxygenase-isoforms are very similar, recent small-angle X-ray scattering data suggested a higher degree of motional flexibility of mammalian isozymes in aqueous solutions. (univaq.it)
Regulation1
- Regulation of arachidonate 12-lipoxygenase and its role in tumor cell alpoptosis " by Keqin. (wayne.edu)
Activity2
- The lipoxygenases are related to each other based upon their similar genetic structure and dioxygenation activity. (wikipedia.org)
- At least 92% of the lipoxygenase activity is localized in the germ. (cerealsgrains.org)
Specific1
- The presence of one lipoxygenase-active electrophoretic band was detected by a lipoxygenase- specific staining procedure. (cerealsgrains.org)
Activation1
- The generation of MMP2 is mediated by activation of phospholipase A(2) and 5-lipoxygenase. (ox.ac.uk)
Role1
- 12-Lipoxygenase plays a key role in cell death caused by glutathione depletion and arachidonic acid in rat oligodendrocytes. (umassmed.edu)
Human2
- Certain types of the lipoxygenases, e.g. human and murine 15-lipoxygenase 1, 12-lipoxygenase B, and ALOXE3, are capable of metabolizing fatty acid substrates that are constituents of phospholipids, cholesterol esters, or complex lipids of the skin. (wikipedia.org)
- Recombinant, human 5-Lipoxygenase expressed in S. fruigiperda insect cells using a baculovirus overexpression system. (sigmaaldrich.com)
Cells1
- Lipoxygenases depend on the availability of their polyunsaturated fatty acid substrates which, particularly in mammalian cells, is normally maintained at extremely low levels. (wikipedia.org)
Description1
- Description of Proton Transfer in Soybean Lipoxygenase-1 Employing App" by James Mazzuca, Sophya V. Garashchuk et al. (sc.edu)
Female1
- Alveolar socket healing in 5-lipoxygenase knockout aged female mice tr" by Ramez H. Mahmoud, Cláudia Cristina Biguetti et al. (utrgv.edu)