• Lipofectamine 3000 reagent outperforms Invitrogen Lipofectamine 2000 and FuGENE HD reagents. (thermofisher.com)
  • Lipofectamine 3000 reagent delivered higher efficiency and protein expression than both Lipofectamine 2000 reagent and Invitrogen Lipofectamine LTX Reagent. (thermofisher.com)
  • Lipofectamine or Lipofectamine 2000 is a common transfection reagent, produced and sold by Invitrogen, used in molecular and cellular biology. (wikipedia.org)
  • Lipofection Transfection Vectors in gene therapy Cationic liposome Invitrogen (2012). (wikipedia.org)
  • Invitrogen Corporation has introduced a key product into its line of transfection reagents. (technologynetworks.com)
  • These technologies add significant capabilities to a transfection portfolio that is already widely adopted throughout the industry and was instrumental in establishing applications such as RNAi and stem cell research," said Claude Benchimol, Ph.D., Senior Vice President of Research and Development for Invitrogen. (technologynetworks.com)
  • Lipofectamine 2000 transfection reagent was produced by Invitrogen (US). (biomedres.info)
  • Trizol reagent was purchased from Invitrogen (US). (biomedres.info)
  • Lipofectamine 2000 reagent (Invitrogen, 11668-027) was used for transfection according to the manufacturer's instructions. (ac.ir)
  • Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. (thermofisher.com)
  • Analysis was performed 48 hours post transfection utilizing flow cytometry to determine percent transfection efficiency and the intensity of GFP expression. (thermofisher.com)
  • Luciferase expression was compared at 24 hours post-transfection using a standard luciferase assay. (bioscience.co.uk)
  • Images (32X) captured at 48 hours post-transfection. (bioscience.co.uk)
  • Images (63X) were captured at 24 hours post-transfection using a Nikon A1R confocal microscope. (bioscience.co.uk)
  • The amount of GAPDH or AHA1 mRNA was measured relative to 18s rRNA levels using qRT-PCR and then normalized to the mRNA levels of the non-targeting control, 48 hours post-transfection. (bioscience.co.uk)
  • A T7E1 mismatch detection assay was used to measure cleavage efficiency at 48 hours post-transfection. (bioscience.co.uk)
  • Lipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. (thermofisher.com)
  • The easy protocol, exceptional plasmid DNA delivery, and the ability to effectively generate stable cell lines makes Lipofectamine 3000 reagent, the perfect reagent for CHO cell transfection experiments. (thermofisher.com)
  • It is used to increase the transfection efficiency of RNA (including mRNA and siRNA) or plasmid DNA into in vitro cell cultures by lipofection. (wikipedia.org)
  • Prepare the plasmid and Lipofectamine-2000 seperately in OptiMEM. (cristian-riccio.ch)
  • Use 3 ug of plasmid and 12 ul of Lipofectamine-2000. (cristian-riccio.ch)
  • TransI T-X2® from Mirus Bio is a low toxicity, dynamic transfection system for the superior transfection of plasmid DNA, siRNA, miRNA and CRISPR/Cas9 components into mammalian and primary cells. (bioscience.co.uk)
  • Herein, we present the first evaluation of cationic dendrimers based on 2,2-bis(methylol)propionic acid (bis-MPA) as nonviral vectors for transfection of short interfering RNA (siRNA) in cell cultures. (mdpi.com)
  • Lipofectamine™ RNAi MAX is a RNAi-specific transfection reagent that is designed to offer high transfection efficiency and low toxicity profiles on a wide variety of cell types for siRNA gene knockdown experiments. (technologynetworks.com)
  • TransIT-X2 and Lipofectamine® 2000 were used to transfect siRNA targeting endogenous proteins - GAPDH and AHA1 or to deliver a nontargeting siRNA control in NHDF. (bioscience.co.uk)
  • Lipofectamine 3000 Transfection Reagent delivers a high efficiency for hard-to-transfect cell lines. (thermofisher.com)
  • When performing transfections in hard-to-transfect cell lines, Lipofectamine 3000 reagent can be an ideal choice. (thermofisher.com)
  • It delivers exceptional transfection efficiency with improved cell viability for the widest range of hard-to-transfect and common cell types including HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines (Figure 1) . (thermofisher.com)
  • Each reagent was used to transfect HeLa cells in a 96-well format at the indicated doses with an emerald green fluorescent protein (GFP)-expressing vector. (thermofisher.com)
  • We were very happy and surprised to see Lipofectamine® 3000 reagent provide a more than 10-fold difference in transfection efficiency in our difficult-to-transfect cell line. (thermofisher.com)
  • Lipofectamine 3000 reagent leverages our most advanced lipid nanoparticle technology to enable exceptional transfection performance and reproducible results. (thermofisher.com)
  • Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine 2000 and FuGENE HD reagents for all five cell lines. (thermofisher.com)
  • The Lipofectamine 3000 transfection protocol was developed to be easy to use, while still ensuring optimum performance and reliability in a wide panel of cell lines. (thermofisher.com)
  • Ribonucleoprotein (RNP) complexes composed of PPIB (cyclophilin B) targeting two part gRNA (IDT) and Cas9 protein (PNA Bio) were delivered into HEK293T/17 and U2OS cells using TransIT-X2® Dynamic Delivery System or Lipofectamine® CRISPRMAX™ or Lipofectamine® RNAiMAX or Lipofectamine® 3000 in a 24-well format according to the manufacturers' protocol. (bioscience.co.uk)
  • The anticancer effect of reagents was examined both in vitro and in vivo using the sulforhodamine B assay and a xenograft mouse model. (biomedcentral.com)
  • Lipofectamine™ LTX Reagent delivers DNA into the cell and demonstrates high levels of transfection efficiency and protein expression with 90 percent viability for a wide range of cells, including primary neuronal cells and other disease-relevant cell types. (technologynetworks.com)
  • Compatible with adherent and suspension cells, the animal-origin free Trans IT-X2® achieves both exceptional transient and stable transfections. (bioscience.co.uk)
  • Lipofectamine™ 2000 is one of our company's top products, and with the advances these new reagents provide, we have a chance to support new and rapidly expanding research applications with the next generation of gene delivery solutions. (technologynetworks.com)
  • Transfection with paxillin Y31FY118F dominant-negative mutant in these cells inhibits ERK activation and restores apoptosis. (rupress.org)
  • But also in non-dividing cells, research has shown that Lipofectamine improves the efficiency of transfection, which suggests that it additionally helps the transfected genetic material penetrate the intact nuclear envelope. (wikipedia.org)
  • 1-3 d after transfection the cells had StemRegenin 1 (SR1) been seeded on 8-μm pore-sized transwell filtration system inserts (Costar) covered with 5 or 10 μg/ml FN or 3 μg/ml VN. (colinsbraincancer.com)
  • Interestingly, transfection of synthetic 22 nucleotide mature single stranded miR-1 (miR-1-3p) induced Cdk9 expression in the fibroblast cells. (ac.ir)
  • Eight µg of both pM-core plasmids and pVP16-ACY3 plasmids, 0.8 µg pG5CAT, and 22 µl of Lipofectamine 2000 reagent were used per plate. (medscape.com)
  • But also in non-dividing cells, research has shown that Lipofectamine improves the efficiency of transfection, which suggests that it additionally helps the transfected genetic material penetrate the intact nuclear envelope. (wikipedia.org)
  • Fig. A comparision of transfection efficiency of Superexpress (#5 & #6) with other popular transfection reagents (#1-#4, #7, #8). (egfie.com)
  • High transfection efficiency in many cell types and formats. (ldbiopharma.com)
  • Product Number QC-2) may be used to assess transfection efficiency. (enquirebio.com)
  • Advantages offered by this technique include shorter transfection timelines, higher biological efficiency, reduced toxicity levels and versatile nucleic acid delivery. (rootsanalysis.com)
  • Vectors and transfection. (calcium-channel.com)
  • Non-viral transfection refers to the targeted delivery of genetic material into a wide variety of cells, using non-viral vectors as the preferred type of carriers. (rootsanalysis.com)
  • Further, considering the growing demand for non-viral vectors and prevalent trends associated with reagents and systems, we believe that this market is likely to witness a steady growth over the coming years. (rootsanalysis.com)
  • The phenotypic profiles of the cells, as well as the expression of histone demethylases, were studied through immunofluorescence, transient transfection, western blot, and qRT-PCR analysis after inducing hypertrophy by angiotensin II and endothelin-1. (hindawi.com)
  • When JMJD2A was overexpressed in cardiomyocytes through transient transfection, the effect of neurohormones on fetal cardiac gene expression was increased. (hindawi.com)
  • Transient transfection was performed using Lipofectamine? (biodiversityhotspot.org)
  • Anti-PODX (3D3) (39-3800) antibody and Lipofectamine 2000 transfection reagent had been purchased from Lifestyle Technology (Carlsbad CA USA). (bioxorio.com)
  • Material and Methods Mice and Reagents Wild type C57BL/6 (6~8 weeks of age) and age-matched male mice were either bred at the UCLA animal facility or purchased from the Jackson Laboratory. (crispr-reagents.com)
  • In order to support the growing demand for nucleic acid-based therapies, novel non-viral transfection reagents and systems, which are deemed to be highly suitable for in vitro intracellular delivery, are being developed. (rootsanalysis.com)
  • As 2 O 3 -induced inhibition in myotube formation and muscle-specific protein expression was reversed by transfection with the constitutively active form of Akt . (nih.gov)
  • TNF alpha knockdown at the protein level was measured at 26 hours post transfection via ELISA. (enquirebio.com)
  • Of these, close to 60 companies have the ability to develop over 185 transfection reagents and transfection kits, that can be used for delivery of genetic materials ( DNA, RNA, nucleic acids and proteins ). (rootsanalysis.com)
  • Affordability: One ml of HyFectin LD1.0 can typically be used for 330 transfections when using 1µg plasmid DNA per well of a 6-well culture plate. (ldbiopharma.com)
  • Materials and techniques Reagents and antibodies. (calcium-channel.com)
  • It is worth highlighting that non-viral transfection reagents and systems are anticipated to become a reliable and powerful tool for artificial introduction of genetic materials. (rootsanalysis.com)