Lipids phosphate binding proteins. Medical search. Frequent questions

The envelope consists of a lipid bilayer that is enriched in sphingomyelin and cholesterol. (hu-berlin.de)
It interacts with the genetic material, with the envelope proteins and with the lipid bilayer of the envelope. (hu-berlin.de)
The molecular building blocks of bilayer membranes are specialized lipids that are polar and nonpolar at their opposite ends. (umn.edu)
Simulations estimating protein-membrane interaction strength for PGZL1 variants along an inferred maturation pathway show bilayer affinity is evolved and correlates with neutralization potency. (elifesciences.org)
Antibodies can target epitopes on integral membrane proteins very near to the lipid bilayer surface, even those partially embedded within the headgroup region. (elifesciences.org)
In cell biology , a vesicle is a structure within or outside a cell , consisting of liquid or cytoplasm enclosed by a lipid bilayer . (wikipedia.org)
In this case, the close proximity to the large-area surface and/or between the consecutively-connected proteins of the tandem repeat can hinder membrane protein studies since the lipid bilayer mimetics such as bicelles or vesicles of ∼100 nm or larger in diameter will not be properly accommodated in the narrow space 19 , 33 . (biorxiv.org)

Proteins are macronutrients when consumed as supplements may exhibit favorable effects on growth metabolism and health [ 5 ] [ 6 ] . (encyclopedia.pub)
Phosphatidic acid (PA) is an important intermediate in membrane lipid metabolism that acts as a key component of signaling networks, regulating the spatio-temporal dynamics of the endomembrane system and the cytoskeleton. (frontiersin.org)
Sec14 like PITPs couple lipid metabolism with phosphoinositide synthesis to regulate Golgi functionality. (tamu.edu)
An interface coordinating lipid metabolism with proteins that regulate membrane trafficking is necessary to regulate Golgi morphology and dynamics. (tamu.edu)
Importantly, Sec14 and Sec14-like proteins are a unique superfamily of proteins that sense specific aspects of lipid metabolism, employing this information to potentiate phosphoinositide production. (tamu.edu)
Therefore, Sec14 and Sec14 like proteins form central conduits to integrate multiple aspects of lipid metabolism with productive phosphoinositide signaling. (tamu.edu)
This battery of measurements are used in the diagnosis and treatment of certain liver, heart, and kidney diseases, acid-base imbalance in the respiratory and metabolic systems, other diseases involving lipid metabolism and various endocrine disorders as well as other metabolic or nutritional disorders. (cdc.gov)
Here, we report that sumoylation of PPARγ couples lipid metabolism to tumor suppressive function of the receptor in lung cancer. (oncotarget.com)
More importantly, it turns out that PPARγ regulation of lipid metabolism was dependent on sumoylation of PPARγ. (oncotarget.com)
Therefore, liganded PPARγ sumoylation is not only critical for cellular lipid metabolism but also induces oxidative stress that contributes to tumor suppressive function of PPARγ. (oncotarget.com)
This study provides an important insight of future translational and clinical research into targeting PPARγ regulation of lipid metabolism in lung cancer patients accompanying type 2 diabetes. (oncotarget.com)
In PE, lipid metabolism is altered. (biomedcentral.com)
Abteilung NMR-Spektroskopie, Weinbergweg 22, DE- and differentiation and in lipid metabolism. (lu.se)

Pleckstrin homology (PH) domains are common membrane-targeting modules and their best characterized ligands are a set of important signaling lipids that include phosphatidylinositol phosphates (PtdInsPs). (rcsb.org)

ORP5 and ORP8: Sterol Sensors and Phospholipid Transfer Proteins at Membrane Contact Sites? (nih.gov)

or a shift of phosphate from the extracellular to the intracellular space. (medscape.com)
Most often it is caused by long-term, relatively low phosphate intake in the setting of a sudden increase in intracellular phosphate requirements. (medscape.com)
Phosphate is a predominantly intracellular anion with a concentration of approximately 100 mmol/L, although determination of the precise intracellular concentration has been difficult. (medscape.com)
Most intracellular phosphate is either complexed or bound to proteins and lipids. (medscape.com)
however, because the intracellular concentration of phosphate is greater than the extracellular concentration, phosphate entry into cells requires a facilitated transport process. (medscape.com)
Several sodium-coupled transport proteins have been identified that enable intracellular uptake of phosphate by taking advantage of the steep extracellular-to-intracellular sodium gradient. (medscape.com)
Such proteins include sphingolipid transfer proteins that function as transporters to shuttle sphingolipids between intracellular membranes as well as lipid-binding domains (e.g. (umn.edu)
This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors that play a key role in the maintenance of cholesterol balance in the body. (nih.gov)
For a more unknowing glutamate of the flash mannose, make the dimerization to the intracellular RNA Pol II glucose lipid well. (evakoch.com)
I-FABP) and bovine heart fatty acid-binding pro- tissues and in intracellular as well as extracellular tein (H-FABP). (lu.se)
for example, H-FABP makes up 3% of the plasmic intracellular LBPs from three different cytoplasmic protein content in rat heart. (lu.se)

Through these interactions, PH domains play a role in recruiting proteins to different membranes, thus targeting them to appropriate cellular compartments or enabling them to interact with other components of the signal transduction pathways. (embl.de)
Interestingly, there are many more varieties of lipids found in membranes than are needed to form bilayers. (umn.edu)
The discovery of these membrane lipid functions underscores why cell membranes so often come under direct attack during cancer and infectious disease. (umn.edu)
The findings have proven to be important for current understanding how the spatial organization of lipids in membranes can regulate proteins that translocate onto membranes to function. (umn.edu)
These sphingolipid transfer proteins were initially identified as glycolipid transfer proteins (GLTPs) due to their specificity for transferring glycosphingolipids between membranes. (umn.edu)
WDFY2 localizes to endosomal tubules by binding to membranes enriched in phosphatidylinositol 3-phosphate (PtdIns3P). (nature.com)
WDFY2 localizes to actin-stabilized endosome tubules positive for the small GTPase RAB4 and shows a preference for highly curved membranes enriched for the lipid phosphatidylinositol 3-phosphate (PtdIns3P). (nature.com)
All-atom simulations of 4E10, PGZL1, 10E8 and LN01 docked onto HIV-like membranes consistently form phospholipid complexes at key complementarity-determining region loop sites, solidifying that stable and specific lipid interactions anchor bnAbs to membrane surfaces. (elifesciences.org)
Conversely, propensity to bind lipids or cell membranes poses a significant auto-immunity risk. (elifesciences.org)
While there has been a large amount of research into the phase behavior of lipid membranes in the past decade driven by the biological relevance of ordered micro-domains (often termed "lipid-rafts"), images of three coexisting phases are very rare. (biophysics.org)
Concurrent decrease in phosphatidylethanolamine content from 23% of total phospholipid to 7%, accumulation of 14C-PL to 2.6% of total phospholipid, and increase in lysophosphatidylethanolamine from 1 to 7% of total phospholipid durkng 2-hexanone + 14CHCl3 treatment indicated that the amine moiety of phosphatidylethanolamine polar head groups was the probable target of phosgene -lipid interaction, and that a degradative pathway existed which removed the abnormal phospholipid from hepatic membranes. (cdc.gov)

Regulators of small G-proteins like guanine nucleotide releasing factor GNRP (Ras-GRF) (which contains 2 PH domains), guanine nucleotide exchange proteins like vav, dbl, SoS and Saccharomyces cerevisiae CDC24, GTPase activating proteins like rasGAP and BEM2/IPL2, and the human break point cluster protein bcr. (embl.de)

The domain family possesses multiple functions including the abilities to bind inositol phosphates, and various proteins. (embl.de)
20. Structural analyses of inositol phosphate second messengers bound to signaling effector proteins. (blindlab.org)
How these molecules transduced the effects of agonists of PI-3K was unclear until the recent discovery that several protein kinases become activated upon exposure to 3'-phosphorylated inositol lipids. (northwestern.edu)

Although wild-type KRAS4b, a common splice variant of KRAS (hereafter referred to as RAS), does not dimerize on two-component supported lipid bilayers( 16 ), it preferentially co-localizes with anionic lipids in the liquid-disordered domains of giant unilamellar vesicles( 17 ) and the oncogenic G12V mutant clusters on the scale of tens of nanometers in extracted PM sheets( 12, 18 ). (researchsquare.com)
What is now clear is that certain membrane lipids can function as messenger signals that regulate cell growth, proliferation, inflammation, and programmed cell death processes, while other membrane lipids appear to cluster together in bilayers to form microdomains that regulate the spatial distribution and lateral interactions of membrane proteins. (umn.edu)
A potent class of HIV-1 broadly neutralizing antibodies (bnAbs) targets the envelope glycoprotein's membrane proximal exposed region (MPER) through a proposed mechanism where hypervariable loops embed into lipid bilayers and engage headgroup moieties alongside the epitope. (elifesciences.org)
Then, evolving tolerance or even affinity to lipid bilayers could be beneficial in recognition of membrane-proximal epitopes, for example gaining avidity and specificity via cooperative interactions simultaneously with the epitope and membrane. (elifesciences.org)
Interestingly, most of these bnAbs show affinity for lipid components and freely associate with lipid bilayers 5 - 8 or cultured cells, even in the absence of antigen 17 - 23 . (elifesciences.org)
Conversely, mutations reducing CDR-H3 hydrophobicity (e.g. 4E10 H100-H102 Trp-Trp motif to Ala-Ala or Asp-Asp) only marginally affect antigen affinity, but drastically reduce neutralization activity and weakened association to lipid bilayers in parallel 7 , 21 , 28 , 29 . (elifesciences.org)

RAS proteins function as molecular switches whose active conformations, stabilized by GTP binding, interact with several protein effectors to control cell growth, proliferation, differentiation, and migration( 10 ). (researchsquare.com)
Heterotrimeric G-proteins are molecular switches that are omnipresent in animal and plant cells. (biophysics.org)
HaloTag-chloroalkane, thiol-maleimide, etc ), at least one end of a protein or its tandem repeat is attached to a solid support without using long molecular spacers, such as DNA handles 25 - 32 . (biorxiv.org)
On the other hand, single-molecule tweezers using hundreds-of-nm long molecular handles flanking a protein of interest still rely on the conventional noncovalent tethers of the dig-antidig and biotin-streptavidin 10 - 15 , 19 . (biorxiv.org)
The long term goal of our research is to elucidate the molecular mechanisms by which proteins are targeted to specific and distinct compartments. (stanford.edu)

In response to kinases and phosphatases, these phosphate ions attach and detach from different molecules, forming a constantly shifting pool. (medscape.com)
Because the fluorescent secondary molecule is applied after capture of the free receptor from solution (Figure 2) the binding constants measured using a kinetic exclusion assay are for unmodified molecules in solution and thus more accurately reflects endogenous binding interactions than methods requiring modification (typically labeling or immobilization) before measurement. (wikipedia.org)
Kinetic exclusion assays have been performed using unpurified molecules, in serum, and have measured binding to cell membrane proteins on intact whole cell which brings the measured binding interactions closer to their endogenous state. (wikipedia.org)
In the process of engaging the antigen, antibodies complementarity-determining regions (CDR) may need to navigate interactions with or concealment by lipid molecules. (elifesciences.org)
Lipid signaling employs various messenger molecules that carry out communication between the plasma membrane, the endomembrane compartments, and the cytoplasm. (frontiersin.org)
Transport vesicles can move molecules between locations inside the cell, e.g., proteins from the rough endoplasmic reticulum to the Golgi apparatus . (wikipedia.org)
Used the protein structure database (PDB) to identify structural motifs common to proteins known to interact with inositide signaling molecules. (blindlab.org)
While a plethora of extracellular molecules exist that modulate cellular functions via binding to membrane receptors inside the cell, their actions are mediated by relatively few signalling mechanisms. (northwestern.edu)
Frankfurt am Main, Germany about half of the crystallographically visible water molecules appear to be expelled by the ligand, we find that ligand binding actually increases the number of water molecules within the cavity. (lu.se)

Phosphoinositides (PPIs) together with various kinases and phosphatases were described as specific lipids and enzymes with signaling functions earlier ( Martin, 1998 ). (frontiersin.org)

The most common assay for phosphate determination in clinical laboratories is based on a spectrophotometric method of complexing the serum inorganic phosphate with ammonium molibdate at low pH and formation of an absorbent phosphomolibdate complex that is measured by the spectrophotometer instrument. (medscape.com)

For example, functional events in RAS dynamics may preferentially depend on local depletion or enrichment of specific lipids are extremely difficult to observe directly either in computational or biological experiments. (researchsquare.com)
This communication system is based on specific lipids as messengers and enzymes responsible for their production and degradation. (frontiersin.org)

Predicted to enable calcium ion binding activity and sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity. (nih.gov)
Predicted to be involved in glycerol catabolic process and glycerol-3-phosphate metabolic process. (nih.gov)
Predicted to be part of glycerol-3-phosphate dehydrogenase complex. (nih.gov)
Orthologous to human GPD2 (glycerol-3-phosphate dehydrogenase 2). (nih.gov)

Phosphate is an integral component of the nucleic acids that comprise DNA and RNA. (medscape.com)

Preferential interaction of RAS with anionic lipids is mediated by 11 positively charged lysines in its HVR( 19 ). (researchsquare.com)
While the broad heterogeneity in lateral diffusion observed by SPT (Fig. 1 A) is indicative of multiple RAS sub-populations that have distinct patterns of interaction with lipids and other cellular components, it provides little insights on local lipid environments and their effect on RAS behavior. (researchsquare.com)
Single-molecule tweezers are an emerging tool for membrane protein studies, which has yielded important insights into membrane protein biogenesis, interaction with drugs, and chaperone effects on their folding 1 - 3 . (biorxiv.org)
This download is the types and cells led from a human assembly target content soccer methylated alongside the set of two interaction localizing enzyme proteins in New Zealand. (evakoch.com)

Whey proteins are rich in bioactive peptides, possessing bioactive properties such as being antioxidant and antihypertensive as well as having antimicrobial activities, which, when ingested, confers several health benefits. (encyclopedia.pub)
Milk provides a wide range of biologically active components such as bioactive proteins and peptides, oligosaccharides, immunoglobulins, and fats/lipids that protect against pathogens and illnesses on regular consumption. (encyclopedia.pub)
These properties are because of the presence of milk proteins/peptides, which support infant development, its growth, and confers health benefits beyond basic nutrition [ 2 ] . (encyclopedia.pub)
The bioavailability of dietary zinc is dependent upon the digestion of these proteins to release zinc and allow it to bond to peptides, amino acids, phosphates and other ligands within the intestinal tract. (scirp.org)

The addition and deletion of phosphate groups to enzymes and proteins are common mechanisms for the regulation of their activity. (medscape.com)
The milk protein system is constituted majorly by two kinds of proteins: approximately 80% (w/w) casein, which is generally extracted from skim milk through precipitation using either an acid (isoelectric precipitation) or enzymes (rennet coagulation) and 20% whey, which is a leftover byproduct after the casein is extracted [ 3 ] . (encyclopedia.pub)
Using tobacco pollen tubes as a model, we addressed the signaling effects of PA by probing the functions of three most relevant enzymes that regulate the production and degradation of PA, namely, phospholipases D (PLD), diacylglycerol kinases (DGKs), and lipid phosphate phosphatases (LPPs). (frontiersin.org)
Phylogenetic analysis indicated a highly dynamic evolution of all three lipid-modifying enzymes in land plants, with many clade-specific duplications or losses and massive diversification of the C2-PLD family. (frontiersin.org)
The synthesis of the peptide stem of bacterial peptidoglycan involves four enzymes, the Mur ligases (MurC, D, E and F). Among them, MurD is responsible for the ATP-dependent addition of d -glutamic acid to UDP-MurNAc- l -Ala, a reaction which involves acyl-phosphate and tetrahedral intermediates. (degruyter.com)
These enzymes include protein kinase B (PKB)/AKT and PtdIns(3,4,5)P 3 -dependent kinases 1 and 2, the first two of which interact with 3'-phosphorylated phosphoinositides via pleckstrin homology domains. (northwestern.edu)

It contains a lipid-binding FYVE domain and seven WD40 repeats which can form a β-propeller. (nature.com)

Pleckstrin, the protein where this domain was first detected, is the major substrate of protein kinase C in platelets. (embl.de)
In addition, the peroxisome proliferator-activated receptor, phosphatidylinositol-3-kinase-protein kinase B, and mammalian target of rapamycin pathways were enriched in the pathway analysis. (animbiosci.org)
17. Crystallographic and kinetic analyses of human IPMK reveal disordered domains modulate ATP binding and kinase activity. (blindlab.org)
Moreover, the Nogo-A-Δ20 domain promotes neuroblast migration toward the OB through activation of the Rho/ROCK (Rho-associated, coiled-coil containing protein kinase) pathway, without the participation of NgR1. (jneurosci.org)
One of these is activation of phosphatidylinositol 3-kinase (PI-3K), which results in the generation of a membrane-restricted second messenger, polyphosphatidylinositides containing a 3'-phosphate. (northwestern.edu)
The generation of effective transdominant mutants, coupled with genetic analysis of the protein kinase in simpler organisms, should help in elucidating outstanding questions in the functions, targets and regulation of this important mediator of PI-3K signalling. (northwestern.edu)

Degradation of high-energy phosphates [ 36 ] and decreased mitochondrial respiratory control [ 21 ] were demonstrated in exercised muscle, indicating the energetic impairment. (scielo.org.ar)
The specific functions of mitoNEET/CISD1 remain to be fully elucidated, but the protein is involved in regulating mitochondrial bioenergetics in several metabolic diseases. (researchgate.net)
Further biochemical analysis revealed that PPARγ-mediated lipid synthesis depletes nicotinamide adenine dinucleotide phosphate (NADPH), consequently resulting in increased mitochondrial reactive oxygen species (ROS) level that subsequently disrupted REDOX balance in lung cancer. (oncotarget.com)

His main research topics have been the design, synthesis, and study of the binding modes of peptidoglycan biosynthetic pathway inhibitors. (degruyter.com)

Mouse protein citron, a putative rho/rac effector that binds to the GTP-bound forms of rho and rac. (embl.de)
These lipid fingerprints are coupled to RAS dynamics, predicted to influence effector binding, and therefore may be a mechanism for regulating cell signaling cascades. (researchsquare.com)
Even less is known about the influence of RAS-lipid coupling on RAS self-assembly and effector activation. (researchsquare.com)

E. coli-derived human Abhd5 recombinant protein (Position: R169-D349). (thermofisher.com)
Our methods can be used to understand the lipid specificities of all yeast SNX-BARs or to produce recombinant SNX-BAR proteins from any other organism. (jove.com)

EPIC: software toolkit for elution profile-based inference of protein complexes. (nih.gov)
The genetic material is organized as bundle of eight ribonucleoprotein complexes that encode for eleven proteins. (hu-berlin.de)

We found that ligand activation of PPARγ dramatically induced de novo lipid synthesis as well as fatty acid beta (β)-oxidation in lung cancer both in vitro and in vivo . (oncotarget.com)
Goethe-Universita¨t, DE-60439 tein (I-LBP) and intestinal fatty acid-binding protein (I-FABP). (lu.se)
I-LBP, ileal lipid-binding to the aqueous solubility of the fatty acid. (lu.se)

Here, we have studied the function of WDFY2, a cytosolic protein that has been described to reside on endocytic vesicles close to the plasma membrane 6 . (nature.com)
These proteins travel within the cell inside of transport vesicles. (wikipedia.org)

Our research elucidated sphingolipid structural features that regulate their interactions with other membrane lipids and provided insights into the unique physical features at the heart of the lateral organizing functionality of sphingolipid-enriched microdomains. (umn.edu)
Vitamin D is a lipid-soluble hormone and micronutrient whose primary function is to regulate calcium and phosphorus homeostasis. (karger.com)
Oxysterol-binding protein related-proteins (ORPs) 5 and 8 regulate calcium signaling at specific cell compartments. (nih.gov)
The data show how the acyl chains regulate SF-1 function in vitro, improving our understanding of how lipids can regulate gene expression in vivo, particularly in the adrenals, gonads & ventral medial hypothalamus. (blindlab.org)

VCP identifies ubiquitylated substrates through numerous dedicated adaptor proteins and unfolds substrates by threading them through a central pore in the hexamer ( Figure 1 ). (portlandpress.com)
VCP interacts with adaptor proteins to identify ubiquitylated substrates for degradation by the proteasome. (portlandpress.com)

The bulk of total body phosphate resides in bone as part of the mineralized extracellular matrix. (medscape.com)

Esmaeili R, Zhang M, Sternberg MR, Mapango C, Pfeiffer CM . A new multiplex immunoassay for serum ferritin, soluble transferrin receptor, C-reactive protein, α-1-glycoprotein, and retinol-binding protein showed variable comparability with traditional reference assays external icon . (cdc.gov)

I continued at the University of Wisconsin to complete my Master of Science degree in Soil Science in 2010, where the research for my thesis focused on the synthesis, detection, and application of phosphate ion labeled with the 18O isotope. (columbiabasin.edu)

Although 14CHCl3-derived radiolabel was irreversibly bound to hepatic macromolecules of both CO- and 2-HX-pretreated rats, total irreversibly bound 14C was significantly enhanced in 2-HX-pretreated rats at all time points. (cdc.gov)

Phosphate is critical for a remarkably wide array of cellular processes. (medscape.com)
Phosphate bonds of adenosine triphosphate (ATP) carry the energy required for all cellular functions. (medscape.com)
The AAA-ATPase (ATPases associated with diverse cellular activities) valosin-containing protein (VCP), is essential for many cellular pathways including but not limited to endoplasmic reticulum-associated degradation (ERAD), DNA damage responses, and cell cycle regulation. (portlandpress.com)
Ethanol can interfere with cell membrane's ability to act as a barrier, and interrupt key cellular processes such as protein biosynthesis, energy transduction and transport[ 8 ]. (biomedcentral.com)

Several S. cerevisiae proteins involved in cell cycle regulation and bud formation like BEM2, BEM3, BUD4 and the BEM1-binding proteins BOI2 (BEB1) and BOI1 (BOB1). (embl.de)
Further works by [8] have established the fact that Zn transporter (ZnT8) is a key protein for the regulation of insulin secretion from pancreatic Beta-cells. (scirp.org)
Phosphate concentration is characterized by a high physiologic variation, depending on age, gender, physiologic state (eg, pregnancy), and even season (due to the seasonal variation of vitamin D, which is directly involved in the regulation of phosphate concentration). (medscape.com)
24. Lipid regulation of full-length nuclear receptor structure. (blindlab.org)

Hypophosphatemia is defined as a serum phosphate level of less than 2.5 mg/dL (0.8 mmol/L) in adults. (medscape.com)
In addition to serum phosphate studies, serum calcium and magnesium studies can be helpful for identifying underlying causes. (medscape.com)
When rapid elevations of serum phosphate levels are documented, the most urgent associated problem is typically hypocalcemia with tetany, seizures, and hypotension. (medscape.com)
Phosphate concentration in plasma and serum will increased in unseparated specimens stored at room temperature for longer time. (medscape.com)
As phosphates concentration is higher inside RBC, hemolysis will lead to increase in phosphates plasma/serum concentration. (medscape.com)
Chaudhary-Webb M, Schleicher RL, Erhardt JG, Pendergrast EC, Pfeiffer CM. An HPLC ultraviolet method using low sample volume and protein precipitation for the measurement of retinol in human serum suitable for laboratories in low- and middle-income countries external icon . (cdc.gov)

Vitamin D is a hormone regulating not only calcium and phosphate homeostasis but also, at the same time, exerting many other extraskeletal functions via genomic effects (gene transcription) and probably by non-genomic effects as well. (karger.com)
Impairment of the capability of the kidney to produce and reuptake enough calcitriol and therefore maintain vitamin D, phosphate, and calcium homeostasis is one of the principal pathophysiological components of metabolic bone disease in CKD. (karger.com)

To define the localization of WDFY2 in the endocytic pathway, we transiently transfected hTERT-RPE1 cells with green flourescent protein (GFP)-WDFY2 and performed structured illumination microscopy (SIM) together with APPL1 and EEA1 visualized with antibodies (Fig. 1a ). (nature.com)
The modeling platform developed here uncovers insights into lipid participation in antibodies' recognition of membrane proteins and highlights antibody features to prioritize in vaccine design. (elifesciences.org)
The work provides insights into how broadly neutralizing antibodies associate with lipids proximal to membrane-associated epitopes to drive neutralization. (elifesciences.org)
However, the evidence for rules for lipid recognition in antibodies is still incomplete . (elifesciences.org)
Nonetheless, in cases of chronic inflammation and infection as in HIV, rare antibodies with lipid affinity can emerge 5 - 8 . (elifesciences.org)

To gain insights into lipid structural features that control both the lateral and transmembrane distributions of sphingolipids, we have used a combination of biophysical approaches (fluorescence spectroscopy, Langmuir surface balance approaches, calorimetry, NMR). (umn.edu)
The initial findings were published in Nature and PLoS Biology, with subsequent studies appearing in Structure, The Journal of Biological Chemistry, Biophysical Journal, Biochemistry, and Journal of Lipid Research. (umn.edu)
Using our methods, we can determine their precise biophysical properties to facilitate lipid binding and membrane remodeling. (jove.com)

GTP binds to the heterotrimeric protein and thereby switches the signal "on. (biophysics.org)

The 3' replication has freely activated by RNase Z surface, a mature binding in receptors( reviewed in Maraia and Lamichhane 2011). (evakoch.com)

In view of the sheer breadth of influence of this mineral, the fact that phosphate homeostasis is a highly regulated process is not surprising. (medscape.com)

Oxysterol binding proteins OSBP, S. cerevisiae OSH1 and YHR073w. (embl.de)
Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. (nih.gov)

Cytoskeletal proteins such as dynamin (see IPR001401 ), Caenorhabditis elegans kinesin-like protein unc-104 (see IPR001752 ), spectrin beta-chain, syntrophin (2 PH domains) and S. cerevisiae nuclear migration protein NUM1. (embl.de)

Whey Proteins and Its Derivatives" Encyclopedia , https://encyclopedia.pub/entry/3494 (accessed December 10, 2023). (encyclopedia.pub)

VCP primarily identifies ubiquitylated proteins in these pathways and mediates their unfolding and degradation by the 26S proteasome. (portlandpress.com)
Valosin-containing protein (VCP, also p97, or Cdc48p in yeast) is an evolutionarily conserved, homo-hexameric, ubiquitin-selective, AAA-ATPase that functions in numerous ubiquitin-dependent protein quality control pathways. (portlandpress.com)
also, the 5' download volatility trading of the cytoplasm is involved actually by cationic end-product by the RNase proximity water cell, which has a direct RNA( RNA H1 in pathways) and at least 10 Fructose-6-phosphate nucleus( activated in Jarrous 2002, Xiao et al. (evakoch.com)

Here we define the precise Rab29 binding region of the LRRK2 Armadillo domain between residues 360-450 and show that this domain, termed 'Site #1', can also bind additional LRRK2 substrates, Rab8A and Rab10. (stanford.edu)

We have since focused our efforts on specific proteins that can bind and transfer sphingolipids between membrane surfaces and that may help formation and maintain 'raft' microdomains in cells. (umn.edu)

In patients with the group A variant, sphingomyelin and other lipids are stored in the brain in increased amounts, a finding consistent with the neuronopathic features, whereas in patients with the group B form, the nervous tissue does not appear to store sphingomyelin. (medscape.com)

18. Signaling through non-membrane nuclear phosphoinositide binding proteins in human health and disease. (blindlab.org)

Point mutations cluster into the positively charged end of the molecule around the predicted binding site for phosphatidylinositol lipids. (embl.de)
The positively charged area in the N-terminal and the C-terminal domain mediated membrane association of the respective mutant protein. (hu-berlin.de)

Oxysterol binding protein-related protein-5 is related to invasion and poor prognosis in pancreatic cancer. (nih.gov)
Oxysterol-binding protein-related protein 5 (ORP5) promotes cell proliferation by activation of mTORC1 signaling. (nih.gov)

RAS is a signaling protein associated with the cell membrane that is mutated in 30% of human cancers. (researchsquare.com)
The RAS subfamily comprises peripheral membrane proteins with a conserved globular GTP-binding domain (G-domain)( 7 ) that is tethered to the cell membrane by a prenylated ~ 20 residue C-terminal domain called the hypervariable region (HVR)( 8, 9 ). (researchsquare.com)
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
The mechanism of exercise-induced myopathy could be the cell membrane damage by lipid peroxidation and DNA fragmentation in the working muscle cells by free radicals generation. (scielo.org.ar)
The PH domain-containing protein Slm1 from budding yeast Saccharomyces cerevisiae is required for actin cytoskeleton polarization and cell growth. (rcsb.org)
Most of these proteins mature in the Golgi apparatus before going to their final destination which may be to lysosomes , peroxisomes , or outside of the cell. (wikipedia.org)

Phosphate concentration increases by 4-5 mg/dl per day in hemolyzed samples stored at 4°C or RT. (medscape.com)
Prolonged storage at RT and delay testing/separation lead to hydrolysis of phosphate esters (eg, glucose phosphate, creatinine phosphate) and overestimation of phosphate concentration. (medscape.com)
Increased concentration of lipids and/or bilirubin (lipemic and icteric specimens) can interfere with accurate laboratory results. (medscape.com)
Standard solutions contain up to about 25% dextrose, but the amount and concentration depend on other factors, such as metabolic needs and the proportion of caloric needs that are supplied by lipids. (msdmanuals.com)

These results demonstrated that the different IMF contents among pig individuals may be due to the DE lncRNAs and mRNAs associated with lipid droplets and fat deposition. (animbiosci.org)
These small non-coding RNAs derived from their precursor sequences, bind to their target mRNAs and negatively mediate the gene expressions in pre-transcriptional or post-transcriptional stages by impeding mRNA translation [ 12 , 13 ]. (springeropen.com)

Our research focuses on membrane lipids known as sphingolipids and on proteins that interact in non-enzymatic fashion with sphingolipids. (umn.edu)
We recently reported that this PH domain binds PtdInsPs and phosphorylated sphingolipids in a cooperative manner. (rcsb.org)

Abstract The present study aimed to determine the effect of different levels of protein on the growth, body composition, amino acid profile and serology of Channa marulius fingerlings. (bvsalud.org)

Ser/Thr protein kinases such as the Akt/Rac family, the beta-adrenergic receptor kinases, the mu isoform of PKC and the trypanosomal NrkA family. (embl.de)
13. Structure of Liver Receptor Homolog-1 (NR5A2) with PIP3 hormone bound in the ligand binding pocket. (blindlab.org)
First structure of the nuclear receptor LRH-1 bound to an endogenous mammalian ligand. (blindlab.org)
ELAC1 has been in the use and may as define as an RNase Z. In pyrophosphates subfamilies are transcribed from coupling tubules in the function by a two receptor chromatin that appears ultraviolet from protein threatening( reviewed in Popow et al. (evakoch.com)

The off-switch is maintained by hydrolysis of GTP to GDP and a phosphate moiety. (biophysics.org)

These findings may have significant implications for the strength, specificity and kinetics of lipid binding. (lu.se)

Total protein, glucose and globulin were also highest in fish feeding 30% CP feed, while albumin was highest in 40% CP feed. (bvsalud.org)

Chemicals and Drugs13

Phenomena and Processes6

Technology, Industry, Agriculture1

Information Science2

Bilayer7

Metabolism13

Phosphatidylinositol phosphates1

Phospholipid Transfer1

Intracellular11

Membranes11

Nucleotide1

Inositol3

Bilayers6

Molecular5

Molecules9

Kinases and phosphatases1

Assay1

Specific lipids2

Glycerol-3-phospha4

Nucleic acids1

Interaction4

Peptides4

Enzymes6

Seven WD40 repeats1

Kinase6

Mitochondrial3

Biosynthetic1

Effector3

Recombinant2

Complexes2

Fatty acid3

Vesicles2

Regulate4

Adaptor proteins2

Extracellular1

Retinol binding1

Synthesis1

Macromolecules1

Cellular4

Regulation4

Serum6

Calcium2

Antibodies5

Biophysical3

Heterotrimeric1

Receptors1

Homeostasis1

OSBP2

Cytoskeletal1

20231

Pathways3

Residues1

Membrane surfaces1

Sphingomyelin1

Phosphoinositide1

Positively charged2

Oxysterol2

Cell6

Concentration4

MRNAs2

Sphingolipids2

Abstract1

Receptor4

Hydrolysis1

Specificity1

Glucose1