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  • plasma membrane
  • He also discusses whether experiments showing correlation of changes in plasma membrane cholesterol with differentiation and the formation of adherens junctions in endothelial cells are consistent with a model in which lipid rafts influence the regulation of these processes. (sciencemag.org)
  • The phospholipid affinity for the transmembrane surface of the PMCA (plasma-membrane Ca 2+ -ATPase) was determined after incubating the protein or the phospholipids with glucose. (biochemj.org)
  • During sublytic complement attack on human neutrophils, plasma-membrane vesicles are shed from the cell surface as a cell-protection mechanism. (biochemj.org)
  • The ganglioside GM1 binds cholera toxin (CT) on host cells and carries it retrograde from the plasma membrane (PM) through endosomes, the trans-Golgi network (TGN), and the endoplasmic reticulum (ER) to induce toxicity. (upenn.edu)
  • close resemblance
  • The results can, in a large part, be transferred to the Rhesus proteins from mammals," says Andrade as Amt proteins bear a close resemblance to the Rhesus proteins found in humans. (phys.org)
  • Here we report the first evidence of a highly swollen gyroid cubic phase under high pressure (gyroid type structures have been seen in ER and Golgi membranes) and the close resemblance of lipid nanostructural behavior with literature studies on compression-decompression of piezophilic biomembranes. (uclan.ac.uk)
  • erythrocyte membrane
  • An increase in phosphatidylcholine content in the erythrocyte membrane from 90 to 282 nmol per 100 μl packed cells was observed. (uu.nl)
  • The nonspecific lipid transfer protein used here appeared to be a suitable tool to modify lipid content and composition of the erythrocyte membrane, and possible applications of this approach are discussed. (uu.nl)
  • PATIENTS AND METHODS: We investigated the correlation between the degree of peroxidation of erythrocyte membrane lipids, erythrocyte alpha tocopherol content, erythrocyte glutathione peroxidase activity and serum albumin concentration in twelve patients with uremia not undergoing hemodialysis and fifteen patients on maintenance hemodialysis. (pptaglobal.org)
  • A significant negative correlation was observed between the erythrocyte alpha tocopherol content and the degree of erythrocyte membrane lipid peroxidation in chronic hemodialysis patients. (pptaglobal.org)
  • There was a statistically significant difference in the degree of erythrocyte membrane lipid peroxidation between patients with chronic hemodialysis-associated hypoalbuminemia and chronic hemodialysis patients having normal serum albumin levels. (pptaglobal.org)
  • CONCLUSION: This study suggested that serum albumin inhibits peroxidation of erythrocyte membrane lipids and that hemodialysis induces recovery of serum reductivity. (pptaglobal.org)
  • specificity
  • Of the lipids tested, S-TGA-1, a halobacterium-derived glycolipid, had the highest specificity to bR with a nanomolar dissociation constant. (elsevier.com)
  • cholesterol
  • The functions of cholesterol and membrane microdomains in transmembrane signaling remain controversial. (sciencemag.org)
  • In contrast, the molar proportions of cholesterol and diacylglycerol relative to sphingomyelin were almost twice those found in plasma membranes. (biochemj.org)
  • In mammalian cells, both the synthesis and uptake of cholesterol are regulated by sterol regulatory element-binding protein transcription factors ( Espenshade and Hughes, 2007 ). (rupress.org)
  • linearly
  • nβ ) 22) monomeric E. coli outer membrane proteins, the stoichiometry of motionally restricted lipids increases linearly with the number of β-strands, the tilt (β ∼ 44°) of the β-strands is comparable for the three proteins, and the order parameter of the β-barrel increases regularly with nβ. (uni-konstanz.de)
  • Spatial distributions of ENTH (epsin N-terminal homology) domain and N-BAR (Bin-Amphiphysin-Rvs) domains derived from the proteins Endophilin and BIN-1 were found to vary approximately linearly with membrane curvature. (upenn.edu)
  • curvature
  • In this contribution, we first investigated the lipid and protein organizations as a function of membrane curvature. (upenn.edu)
  • To this end, a system consisting of solid-supported wavy membranes that exhibits a continuous curvature distribution with positive and negative curvature ranges was fabricated. (upenn.edu)
  • Additionally, to bridge the connection between thermodynamics to highly dynamic cellular environments, we developed a single liposome-based kinetics system which allowed us to examine membrane binding kinetics of proteins as a function of membrane curvature. (upenn.edu)
  • conformational
  • Kinetic and thermodynamic studies indicate that significant conformational changes can be induced in proteins encountering surfaces, which can play a critical role in nucleating aggregate formation or stabilizing specific aggregation states. (frontiersin.org)
  • Often referred to as protein conformational or misfolding disorders, such diseases include Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease (HD), amyloidoses, α1-antitrypsin deficiency, and the prion encephalopathies to name a few. (frontiersin.org)
  • 2DIR spectroscopy measures snapshots of protein conformational ensembles with subpicosecond time resolution. (uoregon.edu)
  • structures
  • Potential parameters modulating protein and lipid distributions in different organelles include membrane shapes and the structures of lipids and proteins. (upenn.edu)
  • Protein loaded lipid structures were subjected to a wide range of pressures to examine their morphological reorganizations using synchrotron X-ray radiation under stepwise pressure variation and rapid pressure jumps. (uclan.ac.uk)
  • By enabling multiplexed analysis of lipids and membrane proteins using currently available microtiter plate based technologies, there is a tremendous opportunity to study these systems in their native states and thereby improve our knowledge of receptor structures and functions. (sbirsource.com)
  • hydrocarbon
  • Based on the OmpA structure, W and C were introduced in two neighboring β-strands oriented toward the hydrocarbon core of the membrane. (uva.nl)
  • structure
  • Proteins fold into their native structure, which is typically a low free energy configuration. (frontiersin.org)
  • Extending these results to the in vivo situation, we can hypothesize that, under hyperglycaemic conditions, glycation of membrane lipids may cause a significant change in the structure and stability of membrane proteins, which may affect the normal functioning of membranes and therefore of cells. (biochemj.org)
  • In 2005, the Freiburg researchers already threw light on the crystalline three-dimensional structure of a protein of this kind. (phys.org)
  • In this paper we address this issue by describing the structure of the SMA lipid particle (SMALP) using data from small angle neutron scattering (SANS), electron microscopy (EM), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), differential scanning calorimetry (DSC) and nuclear magnetic resonance spectroscopy (NMR). (springer.com)
  • Taken together, the description of the structure of the SMALP forms the foundation for future development and applications of SMALPs in membrane protein production and analysis. (springer.com)
  • decreases
  • Results show that the affinity between PMCA and the surrounding phospholipids decreases significantly after phosphospholipid glycation, but remains unmodified after glycation of the protein. (biochemj.org)
  • We also verified that lipid glycation decreases the affinity of lipids for two other membrane proteins, suggesting that this effect might be common to membrane proteins. (biochemj.org)
  • We found that the presence of cone-shaped diacylglycerol decreases line tension, while the commonly used fluorescent lipid, Texas-Red DHPE tends to increase line tension. (upenn.edu)
  • streptavidin
  • His-Tag, Biotin-Tag) can be applied to immobilize the lipid support to various substrates (e.g. to Au-SAM modified by Ni-NTA or passivated glass substrates offering streptavidin entities). (fz-juelich.de)
  • In contrast, streptavidin and fluorescent lipid analogues exhibited homogenous distributions on wavy membranes. (upenn.edu)
  • assays
  • Such a tool would enable novel lipid-based research and diagnostic assays in a multitude of areas including cancer, diabetes, inflammation, infections, and cardiovascular disease. (sbirsource.com)
  • motifs
  • In addition to a core lipid-binding domain, many LTPs have multiple targeting motifs specific for at least two different organelles ( Olkkonen, 2004 ). (rupress.org)
  • quantitative
  • Therefore, we not only reproduced the specific lipid-bR recognition, but also succeeded in its quantitative evaluation, demonstrating the validity and utility of this method. (elsevier.com)
  • electrophysiology
  • We also evaluated the transfer of KcsA from the NABBs to lipid bilayers using single-channel electrophysiology and found that the functional properties of the channel remained intact. (rupress.org)
  • binds
  • All ORPs contain an OSBP-related domain (ORD) that binds sterols and possibly other lipids. (rupress.org)