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  • regulation
  • Furthermore, the complex regulatory network involved in the drought response was revealed, involving proteins that are associated with signaling transduction, transcription and hormone regulation, redox homeostasis, and frontline barriers. (frontiersin.org)
  • structures
  • Instead, infrared results show that the wheat protein contains 41% alpha-helix and 19% beta-sheet structures, while 40% of the conformation is undefined or composed of turns. (semanticscholar.org)
  • amino
  • As opposed to previous studies on the structure prediction from the amino acid sequence, the results obtained show that plant non specific phospholipid transfer proteins are not almost entirely composed of beta-sheets. (semanticscholar.org)
  • Activity
  • These results suggest that the helical structure is essential for the transfer activity without excluding a possible role of the C-terminal extended structure on the adsorption to phospholipid vesicles. (semanticscholar.org)
  • liposomes
  • However, it is not known whether MPs incorporated in these lipid nanoparticles retain the range of key functional properties, as established with other lipid preparations like liposomes and planar lipid bilayers, which are generally used for single-molecule measurements of the movement of ions and molecules through ion channels and transporters. (rupress.org)
  • Traditional biophysical assays of purified ion channels in lipid bilayers are performed via reconstitution of detergent-solubilized channels into liposomes, followed by fusion of these channel-containing proteoliposomes with the target bilayer. (rupress.org)
  • bilayer
  • Here, we report the successful delivery of an ion channel from these particles, called nanoscale apolipoprotein-bound bilayers (NABBs), to a distinct, continuous lipid bilayer that will allow both ensemble assays, made possible by the soluble NABB platform, and single-molecule assays, to be performed from the same biochemical preparation. (rupress.org)
  • Chernomordik and Kozlov, 2003 ), resulting in the vesicle lipids and proteins getting incorporated into the "expanded" target bilayer. (rupress.org)
  • assays
  • The main advantage of an efficient transfer of channels between platforms is that the same monodisperse NABB system can then be used for both ensemble biochemical assays and single-molecule biophysics. (rupress.org)
  • StARD5
  • This study provides the first report on STARD5 protein expression and distribution in mouse kidney. (docphin.com)
  • Treatment of HK-2 cells with inducers of ER stress increased STARD5 mRNA expression and resulted in redistribution of STARD5 protein to the perinuclear and cell periphery regions. (docphin.com)
  • Since recent reports show elevated ER stress response gene expression and increased lipid levels in kidneys from diabetic rodent models, we tested STARD5 and cholesterol levels in kidneys from the OVE26 type I diabetic mouse model. (docphin.com)
  • Stard5 mRNA and protein levels are increased 2.8- and 1.5-fold, respectively, in OVE26 diabetic kidneys relative to FVB control kidneys. (docphin.com)
  • particles
  • The majority of LCAT (approximately 70%) and the neutral lipid transfer protein, or LTP-1 (approximately 80%), in plasma is associated with Lp(A-I) particles. (springer.com)
  • Zou and McHaourab, 2010 ), there have not been any reports to confirm whether the NABB-embedded proteins can be safely transferred to other bilayers-a result that could extend the use of these particles further, as targeted delivery vehicles for MPs. (rupress.org)
  • molecular basis
  • Moreover, the molecular basis for selective recognition and binding among signaling lipids with phosphate headgroups, namely C1P, phosphatidic acid or their lyso-derivatives, remains unclear. (mskcc.org)