Lipid raft domains caveolin 2. Medical search. Frequent questions

CAV -1 is an integral membrane protein found in caveolae, omega-shaped invaginations of the plasma membrane that form a subdomain of cholesterol- and sphingolipid-rich lipid rafts.Cav1 gene is localized to locus D7S522 of human chromosome 7q31.1In vivo, two isoforms of Cav1 are known to exist: α-caveolin that contains residues 1-178 and β-caveolin that contains residues 32-178. (diff.org)
Cav1 and its phosphorylation can impact on the domain recruitment of signalling protein partners and consequently contribute to the trafficking of signalling proteins to and from the plasma membrane. (diff.org)
Moreover, we identified two structural motifs in transmembrane segments 2 and 4 involved in mTRPA1-cholesterol interactions that are necessary for normal agonist sensitivity and plasma membrane localization. (elifesciences.org)
However, it is currently unknown whether TRPA1 is localized in specific domains of the plasma membrane, or whether cholesterol interacts with specific residues of TRPA1. (elifesciences.org)
The mechanisms by which viruses gain entry into host cells are diverse and include direct penetration through the plasma membrane or endocytic uptake followed by vesicular transport through the cytoplasm and delivery to endosomes and other intracellular organelles [ 2 ]. (biomedcentral.com)
Lipid uptake occurs through caveolae, plasma membrane invaginations formed by caveolins (CAV) and caveolae-associated protein 1 (CAVIN1). (aacrjournals.org)
Considerable controversy arose over the concept that cholesterol/sphingolipid-rich rafts in the T cell plasma membrane serve as a platform for TCR signalling reactions. (biomedcentral.com)
Here we review new biophysical and biochemical data which provide an updated picture of the highly dynamic nanometer-sized cholesterol/sphingolipid-rich raft domains stabilised by protein-networks to form TCR signalling platforms in the T cell plasma membrane. (biomedcentral.com)
Early T cell signalling reactions are embedded in the complex and dynamic lipid bilayer matrix of the T cell plasma membrane and are critically defined by their lateral compartmentalisation in plasma membrane domains [ 1 , 2 ]. (biomedcentral.com)
We will here discuss recent data which provide a detailed picture of the membrane raft characteristics of the plasma membrane domains supporting active TCR signalling protein complexes. (biomedcentral.com)
These complexes assemble into submicron TCR signalling domains in the T cell plasma membrane which were first studied by confocal fluorescence microscopy at the contact zone of a T cell with TCR-activating glass coverslips. (biomedcentral.com)
Video microscopy technology resolving single molecule movement was employed to monitor the dynamic interaction of membrane-associated signalling proteins with TCR signalling clusters in plasma membrane domains. (biomedcentral.com)
These studies tracked the movement of Lck and LAT in the T cell plasma membrane and showed retention of these proteins in the submicron TCR signalling domains and their subsequent release. (biomedcentral.com)
However specific lipid-mediated interactions at the T cell plasma membrane bilayer are also a central functional element in early TCR signalling. (biomedcentral.com)
Numerous intracellular signalling proteins interact with plasma membrane lipids of the cytoplasmic leaflet via specific lipid headgroup-binding domains. (biomedcentral.com)
Confocal fluorescence microscopy was employed to monitor the distribution of TCR signalling plasma membrane sites and to relate their distribution to that of clustered raft markers which show resistance to Triton X 100 detergent solubilisation. (biomedcentral.com)
MARV assembles and buds from the host cell plasma where MARV matrix protein (mVP40) dimers associate with anionic lipids at the plasma membrane inner leaflet and undergo a dynamic and extensive self-oligomerization into the structural matrix layer. (uci.edu)
Elastin autofl uoresence … We were drawn to the question of dysfunctional intracellular trafficking in PAH by observations of an inverse relationship between the levels of the plasma membrane raft/caveolar protein caveolin-1 (cav-1) and development of PAH in the rat/monocrotaline model. (informationalwebs.com)
7,8] Numerous studies have elucidated the molecular and vesicular machineries involved in the trafficking of vasorelevant growth factor and cytokine receptors (as examples, the trafficking of BMPR I and BMPR II, VEGFR, IL-6R and gp130, etc.) from the endoplasmic reticulum (ER) through the Golgi apparatus (abbreviated to Golgi) and thence to the plasma membrane or via the option pathways that bypass the Golgi [Physique 2]. (informationalwebs.com)

So far, most caveolin-related research has been conducted in mammals, but the proteins have also been found in other animals, including Xenopus laevis, Fugu rubripes , and Caenorhabditis elegans . (biomedcentral.com)
Ligand induced phosphorylation of EGF receptor creates docking sites for adaptor proteins, such as EPS15, epsin and AP-2 [ 6 , 7 ]. (biomedcentral.com)
Via direct or indirect binding, adaptors recruit the receptor to special membrane regions which are characterized by a particular composition of cage-proteins and/or -lipids [ 8 , 9 ]. (biomedcentral.com)
In the present study, we examined potential contributory roles of membrane-associated, cholesterol-enriched lipid rafts/caveolae and their constituent proteins (e.g., caveolin-1 [Cav-1]) as potential sites for IL-1β−induced nitric oxide (NO) release in the isolated β-cell. (diabetesjournals.org)
Endocytic/exocytic CELL MEMBRANE STRUCTURES rich in glycosphingolipids, cholesterol, and lipid-anchored membrane proteins that function in ENDOCYTOSIS (potocytosis), transcytosis, and SIGNAL TRANSDUCTION. (lookformedical.com)
Often starting as a phagosome in the endocytic compartment, these bacteria-occupied vacuoles transition into organelles with novel molecular features via remodeling of host proteins and lipids through the highly coordinated actions of bacterial factors [3] [5] . (microbialcell.com)

Caveolin-1 is a protein that in humans is encoded by the CAV1 gene. (wikipedia.org)
Caveolin-1 (Cav1) is a 22 KDa multifunctional scaffolding protein with multiple binding partners that is associated with cell surface caveolae and the regulation of lipid raft domains. (diff.org)
Cav1, via biogenesis of caveolae, is not only essential for lipid uptake and regulation, transcellular transport and signalling in endothelial cells, but also for the entry of certain viruses into mammalian cells (SV40). (diff.org)
Some Cav1 has been localized, in a cell type-specific manner, to the cytosol (for example in skeletal muscle cells and keratinocytes), secretory vesicles (in endocrine and exocrine cells), mitochondria (in airway epithelial cells) and lipid droplets. (diff.org)
Genetic alterations of CAV1N1 and CAV1 modify lipid metabolism and underpin lipodystrophy syndromes. (aacrjournals.org)

Results showed that the disassembly of caveolae/lipid rafts by methyl-cyclodextrin induces an increase in intracellular ROS levels, glucose uptake and VEGFR-2 phosphorylation. (unibo.it)
Here we provide the first evidence to suggest that tyrosine phosphorylation of Cav-1 and subsequent interaction among members of the Ras signaling pathway within the membrane lipid microdomains represent early signaling mechanisms of IL-1β in β-cells. (diabetesjournals.org)
The retention of LAT in the TCR signalling domains depended on the phosphorylation of its tyrosines. (biomedcentral.com)
Perilipin 5 S155 phosphorylation by PKA is required for the control of hepatic lipid metabolism and glycemic control. (uci.edu)
Perilipin 5 (PLIN5) is a lipid-droplet-associated protein that coordinates intracellular lipolysis in highly oxidative tissues and is thought to regulate lipid metabolism in response to phosphorylation by protein kinase A (PKA). (uci.edu)
We detected phosphorylation on S155 and identified S155 as a functionally important site for lipid metabolism. (uci.edu)
FLIM-FRET analysis of protein-protein interactions showed that PLIN5 S155 phosphorylation regulates PLIN5 interaction with adipose triglyceride lipase at the lipid droplet, but not with α-β hydrolase domain-containing 5. (uci.edu)
Thus, we investigated changes in P2X3 receptor distribution in the lipid raft membrane compartment, their phosphorylation state, as well as their function with patch clamping. (biomedcentral.com)
Anantin application caused preferential P2X3 receptor redistribution to the lipid raft compartment and decreased P2X3 serine phosphorylation, two phenomena that were not interdependent. (biomedcentral.com)
Tonic inhibition of P2X3 receptor activity by BNP/NPR-A/PKG pathways occurs via two distinct mechanisms: P2X3 serine phosphorylation and receptor redistribution to non-raft membrane compartments. (biomedcentral.com)

The analysis of detergent-resistant T cell membranes had initiated the concept that early TCR signalling steps take place in cholesterol/sphingolipid-rich raft domains of T cell plasma membranes [ 10 ] (see Box for an overview of the current perception of raft domains in cell membranes). (biomedcentral.com)

The internalization process can occur via clathrin-mediated endocytosis, caveolar/lipid raft-mediated endocytosis, macropinocytosis, or a variety of other still poorly characterized mechanisms [ 2 ]. (biomedcentral.com)

Caveolin-1 regulates anchorage-dependent growth and is a tumour suppressor. (europa.eu)
This objective integrates into a bigger project that also aims to identify molecular mechanisms by which caveolin regulates anchorage-dependent signalling pathways and cell cycle progression and to identify oncogenes that induce constitutive surface localization of rafts (and hence Rac), and/or changes in phosphocaveolin localization. (europa.eu)
Here, we show that stromal CAVIN1 regulates lipid abundance in the prostate cancer microenvironment and suppresses metastasis. (aacrjournals.org)

therefore, host lipids and their biosynthesis pathways have emerged as critical regulators of intracellular replication for vacuolar pathogens both as organelle membrane building blocks as well as nutrients [8] [9] . (microbialcell.com)
The MARV matrix layer confers the virion filamentous shape and stability but how host lipids modulate mVP40 oligomerization is mostly unknown. (uci.edu)

With the use of total internal reflection fluorescence (TIRF) microscopy we found a population of highly mobile vesicular structures near the cellular membrane containing both mTRPA1-mCherry and the lipid raft marker cholera toxin B ( Figure 1A and B , Video 1 ), as well as static areas where both fluorescent probes were colocalized at the membrane. (elifesciences.org)

In addition, 637 up-regulated and 577 down-regulated genes in Caco-2 cells incubated with EWH were detected by RNA-sequencing and the clathrin-dependent endocytosis was the most enriched pathway in KEGG analysis. (bvsalud.org)
Endocytosis of MHC I and MHC II was inhibited by filipin, but only MHC II showed a strong preference for a membrane raft environment in a co-clustering analysis with G M 1. (silverchair.com)
Clathrin-mediated endocytosis is generally accepted to be a major route by which nonenveloped viruses infect cells [ 2 ]. (biomedcentral.com)

Introduction of an additional palmitoylatable Cys at position 2 of the wild-type sequence resulted in a chimera that associated to a larger degree with membranes and displayed decreased activity. (biologists.com)
To determine TRPA1 localization in cellular membranes we stained HEK293T cells transfected with the mTRPA1-mCherry construct with the Vybrant Alexa Fluor 488 Lipid Raft Labeling Kit. (elifesciences.org)
This controversy was founded on the initial definition of rafts as detergent resistant membranes which later turned out to misrepresent many features of cell membrane organisation under physiological conditions. (biomedcentral.com)
Thus, intracellular bacteria frequently encode the capacity to regulate host lipogenesis as well as to modulate the lipid composition of host membranes. (microbialcell.com)

This specific project aims to identify sequences within caveolin involved in regulation of raft internalisation, cell cycle progression and anchorage-dependent cell growth. (europa.eu)
We discuss the impact of such interactions on TRPA1 gating mechanisms, regulation by the lipid environment, and role of this channel in sensory membrane microdomains, all of which helps to understand the puzzling pharmacology and pathophysiology of this channel. (elifesciences.org)

Effect of long term simvastatin administration as an adjunct to ursodeoxycholic acid: evidence for a synergistic effect on biliary bile acid composition but not on serum lipids in humans. (cmdm.tw)
Biliary lipid composition in cholesterol microlithiasis. (cmdm.tw)

The three mammalian genes encoding members of the caveolin family are similar in sequence (Table 1 ). (biomedcentral.com)
One membrane component that is often subverted by vacuolar bacteria is cholesterol - an abundant lipid that mammalian cells produce de novo at the endoplasmic reticulum (ER) or acquire exogenously from serum-derived lipoprotein carriers. (microbialcell.com)

In order to better understand the involvement of VEGF/VEGFR-2 in the redox signal transduction pathway, we evaluated the effect of different compounds able to inhibit VEGF interaction with its receptor by different mechanisms. (unibo.it)
Although the evolutionary history of the caveolin genes has not been clearly defined, there are clues within their sequences and genomic organization to suggest possible mechanisms for their origin. (biomedcentral.com)
Effects of aging and type 2 diabetes on cardiac structure and function: Underlying mechanisms. (ucsd.edu)

Expression and characterization of recombinant caveolin. (wikipedia.org)
Disruption of lipid rafts (e.g., with cyclodextrin) markedly reduced IL-1β−induced gene expression of inducible NO synthase (iNOS) and NO release from β-cells. (diabetesjournals.org)
The expression of EGFR, phosphorylated EGFR (pEGFR), protein kinase B (AKT), phosphorylated AKT (pAKT), extracellular signal regulated kinase (ERK1/2), phosphorylated ERK1/2 (pERK1/2) as well as PD-L1 were assessed by western blotting and immunohistochemistry. (biomedcentral.com)
Influence of Sphingolipids on Expression of the Multidrug Resistance Phenotype1,2. (sphingolipidclub.com)
Re-expression of PLIN5 S155A in the liver of Plin5 liver-specific null mice reduced lipolysis compared with wild-type PLIN5 re-expression, but was not associated with other changes in hepatic lipid metabolism. (uci.edu)
LXRs control lipid-inducible expression of the apolipoprotein E gene in macrophages and adipocytes. (cmdm.tw)

Remarkably, they increase lipid uptake and M2 inflammatory macrophage infiltration in the primary tumors and metastasis to distant sites. (aacrjournals.org)

Lipid and protein content profiling of isolated native autophagic vesicles. (unimedizin-mainz.de)
Figure 2: Roles of extracellular vesicles in normal physiology and disease pathogenesis. (nature.com)

We have analysed the mechanism by which palmitoylation permits the progression of nitric oxide synthase 2 (NOS2) along the ER-Golgi-TGN pathway. (biologists.com)

We show that depletion of CAVIN1 in prostate stromal cells markedly reduces their lipid droplet accumulation and increases inflammation. (aacrjournals.org)

The potential anti-cancer properties of krill oil are likely to be associated with the downregulation of EGFR, pEGFR and their downstream pERK/ERK1/2 and pAKT/AKT signalling pathways along with the downregulation of PD-L1. (biomedcentral.com)

Integrin-regulated Rac binding sites are in lipid rafts (membrane domains enriched in cholesterol and glycosphingolipids). (europa.eu)

A final, intriguing potential is that multiple determinants in the cytoplasmic, transmembrane, or the score with in the extracellular domain of CD4 are liable looking during drawing CD4 into membrane rafts. (upb.ro)

Caveolin sporadic mutations occurring in human cancers will be tested for these effects. (europa.eu)
The borrowing between delivery( EFN) drugs and cyclin Mutations rights positively all in Once gardening through the EPH domain, but also in' gene' enhancing through the EFN region itself. (familie-vos.de)
Mutations in caveolin 3 are associated with multiple muscle diseases including DISTAL MYOPATHY and LIMB-GIRDLE MUSCULAR DYSTROPHY. (lookformedical.com)

Studies suggest that pY14Cav1-mediated control of raft internalization plays an important role in integrin-mediated control of cell proliferation that, when de-regulated, can lead to anchorage-independent growth and transformation and thereby the acquisition of tumorigenic potential. (diff.org)

Caveolae/lipid rafts are membrane-rich cholesterol domains endowed with several functions in signal transduction, and Cav-1 has been reported to be implicated in regulating multiple cancer-associated processes, ranging from cellular transformation, tumor growth and metastasis, to multidrug resistance and angiogenesis. (unibo.it)
It binds CHOLESTEROL and is involved in LIPIDS transport, membrane traffic, and SIGNAL TRANSDUCTION. (lookformedical.com)

Molecules that interact with caveolin-1 contain caveolin-binding motifs (CBM). (wikipedia.org)

the Cav-2 gene also maps to the same chromosomal region as Cav-1 (7q31.1 in humans). (biomedcentral.com)

Caveolin-1 interacts with androgen receptor. (wikipedia.org)
Vascular endothelial growth factor receptor-2 (VEGFR-2) and caveolin-1 (Cav-1) are frequently colocalized, suggesting an important role played by this colocalization on cancer cell survival, migration and proliferation. (unibo.it)
Thus, our attention was directed to a human leukaemia cell line (B1647) that constitutively produces VEGF and expresses the tyrosine-kinase receptor VEGFR-2. (unibo.it)
As the dissociation of VEGFR-2 from caveolae has been shown to be essential for receptor autophosphorylation and activation of downstream signalling events, we investigated the presence and the role of VEGFR-2 in caveolae/lipid rafts, focusing our attention on the correlation between reactive oxygen species (ROS) production and glucose transport modulation by VEGF. (unibo.it)

All these interactions are through a caveolin-scaffolding domain (CSD) within caveolin-1 molecule. (wikipedia.org)

In this study, we show that mouse TRPA1 is located preferably in cholesterol-rich domains and identify cholesterol recognition amino acid consensus (CRAC) motifs in the TM2 and TM4 segments that are implicated in the attenuation of chemical activation of mTRPA1 by cholesterol-depleting agents. (elifesciences.org)

Internalisation of Rac binding sites is mediated by caveolin-1, a structural component of a subtype of rafts termed caveolae. (europa.eu)

Raft-organisation was subsequently studied using a number of detergent-free experimental approaches. (biomedcentral.com)

These results may provide a molecular explanation for the role of caveolin-1 as tumour suppressor and provide insight into the mechanism of anchorage-independent growth, one of the hallmarks of cancer. (europa.eu)

To deeply analyse the role of caveolae/lipid rafts in redox signaling promoted by VEGF, the effect of Cav-1 scaffolding domain peptide was studied. (unibo.it)

Lipids contribute to tumorigenesis by providing fuel to cancer metabolism and supporting growth and signaling. (aacrjournals.org)

The highest levels of caveolin-1 (also called caveolin, Cav-1 and VIP2I) are found in terminally-differentiated cell types, such as adipocytes, endothelia, smooth muscle cells, and type I pneumocytes. (biomedcentral.com)
Caveolin-3 (Cav-3) has greater protein-sequence similarity to Cav-1 than to Cav-2, but it is expressed mainly in muscle cells, including smooth, skeletal, and cardiac myocytes. (biomedcentral.com)
immune lymphocytes like Bcl-2, Bcl-XL and their cells download signal in all TKIs BH1-4. (familie-vos.de)
A caveolin that is expressed exclusively in MUSCLE CELLS and is sufficient to form CAVEOLAE in SARCOLEMMA. (lookformedical.com)
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. (lookformedical.com)
Our data support the concept that stromal cells contribute to prostate cancer aggressiveness by modulating lipid content and inflammation in the tumor microenvironment. (aacrjournals.org)
Acute exposure ofMCF-7 and MDA-MB-231 cells to C8-glucosylceramide (10 μg/ml culture medium), a cell-permeable analog of glucosylceramide, increased MDR1 mRNA levels by 2- and 4-fold, respectively. (sphingolipidclub.com)
Soy protein peptic hydrolysate with bound phospholipids decreases micellar solubility and cholesterol absorption in rats and caco-2 cells. (cmdm.tw)
3] The observations by Zhao and colleagues that mice spontaneously developed pulmonary hypertension and dilated cardiomyopathy,[4,5] and reports that cav-1 and cav-2 were reduced in the cells in plexiform lesions in patients with severe PAH[6] , heightened interest in this inverse relationship. (informationalwebs.com)

Integrin-mediated retention of phosphorylated caveolin in focal adhesions inhibits raft internalisation, which occurs upon its recruitment to caveolae after cell detachment. (europa.eu)

2 Department of Molecular and Cellular Physiology, Louisiana State University Health - Shreveport, Shreveport, LA 71130. (microbialcell.com)

Using total internal reflection fluorescence microscopy and density gradient centrifugation we found that mouse TRPA1 localizes preferably into cholesterol-rich domains and functional experiments revealed that cholesterol depletion decreases channel sensitivity to chemical agonists. (elifesciences.org)

173(Pt 2): 113480, 2023 11. (bvsalud.org)
Epub 2023 Oct 2. (unimedizin-mainz.de)

Marburg virus (MARV) is a lipid-enveloped virus harboring a negative sense RNA genome, which has caused sporadic outbreaks of viral hemorrhagic fever in Sub-Saharan Africa. (uci.edu)

Caveolin-2 (Cav-2) and caveolin-3 (Cav-3) were identified in 1996 using different experimental methods. (biomedcentral.com)

Lipid-specific oligomerization of the Marburg virus matrix protein VP40 is regulated by two distinct interfaces for virion assembly. (uci.edu)

Amphotericin B lipid complex or amphotericin B multiple-dose administration to rabbits with elevated plasma cholesterol levels: pharmacokinetics in plasma and blood, plasma lipoprotein levels, distribution in tissues, and renal toxicities. (cmdm.tw)

Our results suggest that the preference of MHC I and MHC II for distinct membrane rafts directs them to different cellular entry points. (silverchair.com)
Using in vitro and cellular techniques, we present a mVP40 assembly model highlighting two distinct oligomerization interfaces: the (N-terminal domain (NTD) and C-terminal domain (CTD)) in mVP40. (uci.edu)

Moreover, the transepithelial transport of EWH across Caco-2 cell monolayers was significantly reduced by chlorpromazine. (bvsalud.org)

Anatomy11

Organisms3

Chemicals and Drugs36

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Phenomena and Processes27

Technology, Industry, Agriculture5

Information Science3

Plasma membrane19

Proteins6

CAV15

Phosphorylation10

Sphingolipid-rich1

Caveolar1

Regulates3

Host lipids2

Cholera toxin1

Endocytosis3

Membranes4

Regulation2

Composition2

Mammalian2

Mechanisms3

Expression6

Uptake1

Vesicles2

Nitric oxide sy1

Droplet1

Pathways1

Glycosphingolipids1

Cytoplasmic1

Mutations3

Internalization1

Signal transduction2

Molecules1

Humans1

Receptor4

Interactions1

Motifs1

Structural1

Detergent1

Suppressor1

Role1

Metabolism1

Cells9

Retention1

Molecular1

Cholesterol-rich1

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Genome1

19961

Matrix1

Tissues1

Distinct2

Cell1