• Given these findings, AFM24 is now being evaluated in a phase 1/2 trial (NCT04259450) in patients with advanced EGFR -positive solid tumors. (onclive.com)
  • Interleukin 15 (IL-15) has been evaluated as a potential treatment for solid tumors in clinical trials, but the effectiveness of systemic IL-15 administration as a monotherapy has not been realized. (frontiersin.org)
  • Cisplatin (cis-diamminedichloroplatinum II, CDDP) is a chemotherapeutic agent widely used in the treatment of several solid tumors, among them testicular cancer, lung cancer, breast cancer, and bladder cancer. (biomedcentral.com)
  • These results suggest that a clinical vaccine trial using autologous tumor cells genetically modified to secrete IL-2 is feasible in patients with solid tumors. (arizona.edu)
  • We created an in vitro model of acquired cisplatin resistance by long term exposure of three well established germ cell tumor cell lines to cisplatin, resulting in sublines with significantly increased resistance to cisplatin. (biomedcentral.com)
  • To evaluate the biological behavior of carcinomas in vitro, permanent tumour cell lines are required. (elsevierpure.com)
  • Our studies optimize in vitro transfection of primary tumor cells using cationic lipids and a plasmid encoding the gene for human interleukin-2 (IL-2). (arizona.edu)
  • Natural News) In this study, researchers from The First Hospital of China Medical University evaluated the anti-tumor effect and mechanism of action of elemene on pancreatic carcinoma in vitro and in vivo. (naturalnews.com)
  • In vitro experimental models to study the efficiency of the placental barrier for environmental toxicants: tumor cell lines versus trophoblast primary cells. (biomedicineandprevention.com)
  • Several trophoblast cell lines have been developed for in vitro studies of trophoblast physiology and in the following paragraphs we will give a brief overview on the cells have been used to develop in vitro models of the placental barrier. (biomedicineandprevention.com)
  • Several trophoblast cell lines have been developed and used to reproduce in vitro the trophoblast layer of the placenta. (biomedicineandprevention.com)
  • MST-1 cells grown in vitro were heterogeneous in morphology, ranging from floating round cells, loosely attached round/oval or elongated cells with prominent pseudopod-like processes, to well-attached spindle and elongated dendritic cells without obvious pseudopods. (tmu.edu.tw)
  • We found that shRNA knockdown of intrinsic ST6GAL1 expression resulted in decreased ST6GAL1 cargo in the exosome-like vesicles as well as decreased breast tumor cell growth and invasive behavior in 3D in vitro cultures. (nature.com)
  • In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)
  • Medium (200 mm 3 ) and large (800 mm 3 ) tumors were collected and tumor composition was determined by flow cytometry. (genoway.com)
  • Boron neutron capture therapy (BNCT) is a promising method for treating tumors, in particular, infiltrative malignant tumors, due to the selective destruction of tumor cells without damaging the surrounding normal tissues. (isnct.net)
  • Surgery is the primary treatment of malignant tumors of the salivary glands. (medscape.com)
  • Since, apoptosis induced by PDT treatment plays an important role in determining the photodynamic efficacy, in the present work we have investigated the role of apoptotic cell death in relation to the observed differences in sensitivity to HpD-PDT between a human glioma cell line (BMG-1) carrying wild-type tumour suppressor gene p53 and a human squamous carcinoma cell line (4451) with mutated p53. (elsevierpure.com)
  • Peripheral blood mononuclear cells (PBMCs) of various healthy donors were plated in the upper compartment and after six hours the transwell migration was quantified by flow cytometry. (bmj.com)
  • Enzyme-linked immunoabsorbent analysis of the amount of IL-2 secreted into the medium by transfected tumor cells correlated with the percentage of tumor cells expressing intracellular IL-2 as measured by flow cytometry. (arizona.edu)
  • Here we investigated the characteristics of a population of pancreatic cancer tumorspheres derived from different human pancreatic cancer cell lines and a primary line from a genetically engineered KPC mouse model, using flow cytometry and western blotting to analyse surface and stemness markers. (lu.se)
  • More importantly, we show that MESF treatment leads to significant inhibition of tumor growth and a 3-fold increase in the life span of tumor bearing animals compared to untreated tumor mice. (nature.com)
  • Intraperitoneal administration of IL-15cx results in a moderate inhibition of breast cancer growth that is associated with an increase in the frequency of cytotoxic CD8 T cells and the improvement of their function. (frontiersin.org)
  • Systemic and local administration of IL-15cx shows two distinct therapeutic responses, a moderate tumor growth inhibition or heterogeneous tumor regressions with survival improvement. (frontiersin.org)
  • Therefore, to search for indole compounds with more efficacy and less side effect for cancer therapy, we developed a novel indole compound SK228 and examined its effects and mechanisms on antitumor growth and invasion inhibition in cell and tumor xenografts in nude mice models. (ntnu.edu.tw)
  • In this work, for the first time we have demonstrated that ATRA affected on cell proliferation of GIST-T1 and GIST-882 cell line through inhibition of cell growth in a dose dependent manner and induced apoptosis. (biomedcentral.com)
  • To investigate the metastatic potential of K3 cells, we established the nude mouse models of liver and lung metastases and isolated the corresponding metastatic cell lines K3-F4 and K3-B6. (oncotarget.com)
  • Only the has-miR-10b involved in breast cancer invasion and metastasis and has-miR-512-3p appeared to be up-regulated (2-3-fold) in all three cell lines. (biomedcentral.com)
  • Abstracts BackgroundClear cell renal cell carcinoma (ccRCC) cell lines with distinct metastatic potential are crucial to study the mechanism of ccRCC metastasis. (biopaqc.com)
  • No lung metastasis was detected following the first round of surgical orthotopic implantation (SOI) with MRCC and NRCC tumors. (biopaqc.com)
  • Furthermore the metastasis to lymph nodes near ventral aorta and hemorrhagic ascites had been often noticeable in the SOI mice transplanted with MRCC tumors. (biopaqc.com)
  • However metastasis had not been observed on the initial three rounds from the transplantation with NRCC cells. (biopaqc.com)
  • Desk 2 Tumorigenicity and metastasis of both cell lines pursuing orthotopic transplantation in nude mice Debate In this research we successfully set up two ccRCC cell lines from two Chinese language sufferers with ccRCC. (biopaqc.com)
  • N-Myc downstream-regulated gene 1 (NDRG1) plays vital roles in tumor metastasis suppression and is frequently silenced in metastatic colon cancers. (nih.gov)
  • Furthermore, the scracht assay result suggested that ATRA was a potential reagent to prevent the invasion or metastasis of GIST cells. (biomedcentral.com)
  • Here we show that methanolic extracts of Sapota fruit (MESF) induces cytotoxicity in a dose-dependent manner in cancer cell lines. (nature.com)
  • Annexin V-propidium iodide double-staining demonstrated that Sapota fruit extracts potentiate apoptosis rather than necrosis in cancer cells. (nature.com)
  • In a preliminary study it has been shown that phenolic antioxidants such as methyl 4- O -galloylchlorogenate and 4- O -galloylchlorogenic acid derived from Sapota fruits can induce cytotoxicity in colon cancer cells 23 . (nature.com)
  • LINE-1 are poorly expressed in normal cells and abundantly in cancer cells. (unife.it)
  • Decreasing RT activity in cancer cells, by either LINE-1-specific RNA interference, or by RT inhibitory drugs, was previously found to reduce proliferation and promote differentiation and to antagonize tumor growth in animal models. (unife.it)
  • Using CsCl density gradients, we selectively identify Alu and LINE-1 containing DNA:RNA hybrid molecules in cancer but not in normal cells. (unife.it)
  • In cancer cells the abundant RT reverse-transcribes retroelement-derived mRNAs forming RNA:DNA hybrids. (unife.it)
  • The goal of this study was to examine the activity of IL-15/IL-15Rα complex (IL-15cx) to CD8 + T cells and evaluate its potential efficacy in murine breast cancer models. (frontiersin.org)
  • The depletion of myeloid-derived suppressor cells (MDSCs) has no impact on mouse breast cancer growth. (frontiersin.org)
  • One of these poorly immunogenic tumors is breast cancer with response rates of only 0%-18.5% to CPI therapy alone ( 2 - 4 ). (frontiersin.org)
  • PD-L1 is expressed mainly on tumor-infiltrating immune cells in roughly 41% of patients with triple negative breast cancer ( 5 ). (frontiersin.org)
  • IL-15 is considered superior to IL-2 for cancer immunotherapy due to its strong bioactivity in improving CD8 + T and natural killer (NK) cell survival, proliferation and cytotoxic function ( 8 ). (frontiersin.org)
  • Cancer Genome Atlas N. Comprehensive genomic characterization of head and neck squamous cell carcinomas. (bmj.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Moreover, the K3 cells contained side population cells (SP) like cancer stem cells (CSCs), which might contribute to K3 heterogeneity and tumorigenic capacity. (oncotarget.com)
  • This is a list of major breast cancer cell lines that are primarily used in breast cancer research. (wikipedia.org)
  • For more data on the nature of TP53 mutations in breast cancer cell lines, see Lacroix et al. (wikipedia.org)
  • Cancer Cell. (wikipedia.org)
  • Patients in Europe with MSI-H/dMMR colorectal cancer have had only chemotherapy-containing regimens available to them in the first-line treatment setting and have historically faced poor outcomes," said Dr. Vicki Goodman, vice president, clinical research, Merck Research Laboratories. (merck.com)
  • Microsatellite instability (or MSI) is defined by the National Cancer Institute as a change that occurs in the DNA of certain cells, such as tumor cells, in which the number of repeats of microsatellites (short, repeated sequences of DNA) is different from the number of repeats that was in the DNA when it was inherited. (merck.com)
  • It is estimated approximately 5-15% of colorectal cancer patients have tumors that score as either MSI-H or dMMR when testing is performed. (merck.com)
  • Colorectal cancer often begins with growths on the inner lining of the colon or rectum called polyps, which can change into cancer over time. (merck.com)
  • Cancer vaccines that utilize genetically modified tumor cells require gene transfer methods capable of producing immunostimulatory doses of transgenes from fresh or short-term cultures of human tumor cells. (arizona.edu)
  • NDRG1 is silenced in a highly metastatic colon cancer cell line SW620. (nih.gov)
  • SW480 and SW620 are two colon cancer cell lines established from the same patient with different metastatic potentials, making them an ideal model for investigation of metastatic mechanisms. (nih.gov)
  • In conclusion we observed unique histone modifications in two colon cancer cell lines with different metastatic potentials, indicating possible mechanisms for the down-regulation of NDRG1 in metastatic SW620. (nih.gov)
  • There are several types of lung cancer, but non-small cell (NSCLC) is the most common form. (naturalnews.com)
  • According to a study published in Nature Cell Biology, activating transcription factor 4 (ATF4) could be the key to conquering cancer. (naturalnews.com)
  • Molecular and functional profiling of cancer cell lines is subject to laboratory-specific experimental practices and data analysis protocols. (ntnu.no)
  • The current challenge therefore is how to make an integrated use of the omics profiles of cancer cell lines for reliable biological discoveries. (ntnu.no)
  • We demonstrated the power of the integrative analyses by identifying a novel driver gene, ECHDC1, with tumor suppressive role validated both in breast cancer cells and patient tumors. (ntnu.no)
  • The multi-modal meta-analysis approach also identified synthetic lethal partners of cancer drivers, including a co-dependency of PTEN deficient endometrial cancer cells on RNA helicases. (ntnu.no)
  • SK228 significantly inhibited growth of different lung and esophageal cancer cell lines at sub-micromolar range, but not normal lung cells. (ntnu.edu.tw)
  • Moreover, low-dose SK228 significantly reduced the invasion of cancer cells. (ntnu.edu.tw)
  • Our study provides the first evidence that SK228 exhibits cancer cell-specific cytotoxicity by inducing mitochondria-mediated apoptosis. (ntnu.edu.tw)
  • In addition, SK228 inhibits cancer cell invasion via FAK/Paxillin disruption at noncytotoxic doses. (ntnu.edu.tw)
  • Nevertheless at a day following the treatment the AP-1 activity was hardly detectable in Computer3 cells mainly because most the Computer3 cancer tumor cells were inactive at the moment stage (Fig. 2C). (ecologicalsgardens.com)
  • The availability of the soft tissue melanoma cell line, the SCID mouse xenograft tumour system as well as autologous TILs described herein would provide useful materials for identifying T-cell-defined antigens as well as a model system for devising individualised cancer biotherapeutic strategies. (tmu.edu.tw)
  • This cell line can also be used for further studies aimed at uncovering the histogenesis of this rare cancer. (tmu.edu.tw)
  • Extracellular ST6GAL1, present in cancer exosomes or the freely soluble recombinant sialyltransferase, compensates for insufficient intrinsic ST6GAL1 by boosting cancer cell proliferation and increasing invasiveness. (nature.com)
  • It is within the cell-autonomous context that ST6GAL1 involvement in cancer progression has been interpreted. (nature.com)
  • Aggressive breast cancer is a highly heterogeneous disease caused by a variety of distinct cell-intrinsic genetic alterations in mammary epithelial cells, leading to vastly heterogenic disease manifestation in individual patients and predominantly affecting patient prognosis and treatment options [ 14 ]. (nature.com)
  • Cancer cell-extrinsic mechanisms, which are poorly understood, are also believed to contribute to disease progression and the heterogeneous genetic mutations with diverse presentations. (nature.com)
  • In this form, known as diffuse gastric cancer, there is no solid tumor. (medlineplus.gov)
  • however, because this type of cancer is hidden underneath the stomach lining, it is usually not diagnosed until the cancer has become widely invasive. (medlineplus.gov)
  • A lack of E-cadherin also impairs cell adhesion, increasing the likelihood that cancer cells will not come together to form a tumor but will invade the stomach wall and metastasize as small clusters of cancer cells into nearby tissues. (medlineplus.gov)
  • Cancer stem cells are a population of slow-cycling cells within the tumour bulk, with self-renewal capacity that attracts interest as a therapeutic target. (lu.se)
  • In highly heterogeneous tumours, like pancreatic ductal adenocarcinoma (PDAC) however, the characterisation of cancer stem cells has led to controversial results due to the lack of consensus on specific markers. (lu.se)
  • diagnostic prediction of small round blue cell tumors (SRBCT) of childhood and determining the estrogen receptor (ER) status of sporadic breast cancer. (lu.se)
  • Leiomyosarcoma (cancer of smooth muscle cells) can develop in the wall of the small intestine. (msdmanuals.com)
  • Natural News) In an exciting new development, scientists from the University of Pennsylvania's Perelman School of Medicine have found what they term the Achilles' Heel of cancerous tumors. (naturalnews.com)
  • Carcinoid Tumors and Carcinoid Syndrome Carcinoid tumors are noncancerous (benign) or cancerous (malignant) growths that sometimes produce excessive amounts of hormone-like substances (such as serotonin), resulting in the carcinoid. (msdmanuals.com)
  • KEYTRUDA is a humanized monoclonal antibody that blocks the interaction between PD-1 and its ligands, PD-L1 and PD-L2, thereby activating T lymphocytes which may affect both tumor cells and healthy cells. (merck.com)
  • The antitumor efficacy was studied in mouse mammary carcinoma models (Her2/neu transgenic and 4T1-luc mammary cancers) treated with systemic recombinant protein with/without the depletion of myeloid-derived suppressor cells or intra-tumoral gene electrotransfer (GET). (frontiersin.org)
  • Except for the B3B3 line that has undergone unproductive gene rearrangement, all other lines express IgH and L mRNAs but do not synthesize any Ig protein (Robert et al. (rochester.edu)
  • The most frequently methylated gene in both primary tumors and cell lines was RASSF1A (40, 86%, respectively). (elsevierpure.com)
  • Loss of DKK3 gene is associated with a possible tumor suppressor role in human cancers. (amrita.edu)
  • People with HDGC caused by CDH1 gene mutations are born with one mutated copy of the gene in each cell. (medlineplus.gov)
  • involving the other copy of the CDH1 gene must occur in the cells of the stomach lining during a person's lifetime. (medlineplus.gov)
  • When both copies of the CDH1 gene are mutated in a particular cell, that cell cannot produce any functional E-cadherin. (medlineplus.gov)
  • It is unclear why CDH1 gene mutations primarily occur in the stomach lining and these other tissues. (medlineplus.gov)
  • The main emphasis is on supervised machine learning methods for classification and prediction of tumor gene expression profiles. (lu.se)
  • Since genes are composed of a given alphabet (A, T, C, G) with fixed pairing properties A-T and C-G, probes can be constructed that attract gene transcripts extracted from cell tissues and cultures. (lu.se)
  • Infinium data for four matched tumor-normal pairs and a dilution series of a tumor cell line mixed with its paired normal cell line are available in NCBI's Gene Expression Omnibus with accession ​ GSE11976 . (lu.se)
  • Both K3-F4 and K3-B6 cell lines with higher metastatic potential acquired more mesenchymal and stemness-related features. (oncotarget.com)
  • ResultsTwo successive cell lines named NRCC from the primary ccRCC and MRCC from the metastatic ccRCC were established respectively. (biopaqc.com)
  • ConclusionsCancer cells isolated from metastatic ccRCC have more malignant and metastatic potential than those from the primary tumor from the patients who shared the similar race background. (biopaqc.com)
  • Metastatic potential of MRCC and NRCC in nude mice Subcutaneous transplantation of MRCC cells or NRCC cells generated tumors in nude mice within two weeks. (biopaqc.com)
  • We established and isolated the metastatic cell stress from pulmonary tumor mass named MRCC-L. MRCC-L looked smaller sized in proportions and grew quicker than their parental MRCC. (biopaqc.com)
  • Even though microenvironment plays an important role in evolutionary process of the metastatic cells from their main tumors the selected metastatic malignancy cells maintain their characteristics following long-term cultures. (biopaqc.com)
  • study exhibited that MRCC cells exhibited more malignant and metastatic potential than NRCC (Table?2). (biopaqc.com)
  • Knockdown of NDRG1 in SW480 to a level that is similar to that in SW620 also modulated cell cycle and proliferation in SW480 towards the status of the highly metastatic SW620. (nih.gov)
  • Many of these cell lines have been obtained from a highly metastatic malignant tumor of the trophoblast, the choriocarcinoma, and have been extensively characterized. (biomedicineandprevention.com)
  • Human cell cultures: glioblastoma (U87), colorectal human adenocarcinoma (SW-620), human melanoma (SK-Mel28) and primary embryonic cell lines were irradiated with a neutron flux at the presence of BPA, BSH and liposomal BSH with a concentration of10B 40 μg/ml. (isnct.net)
  • The data obtained indicate that the accelerator-based BNCT using boron delivery drugs, such as borphenylalanine, borcaptate and liposomal borcaptat, has a positive effect on tumor lines of glioblastoma, colorectal adenocarcinoma and melanoma. (isnct.net)
  • Growth of an A549 tumor cell xenograft was markedly inhibited without significant side effects. (ntnu.edu.tw)
  • Immunostaining revealed that major melanoma-associated antigens, such as S100 protein, HMB-45, melanotransferrin, chondroitin sulphate proteoglycan, and the gangliosides GD2 and GD3, were consistently expressed by the tumour tissue, severe combined immunodeficiency (SCID) mouse xenograft and derived cell lines. (tmu.edu.tw)
  • Remarkably, hybrid molecules fail to form in tumor cells treated with EFV under the same conditions that repress proliferation and induce the reprogramming of expression profiles of coding genes, microRNAs (miRNAs) and ultraconserved regions (UCRs). (unife.it)
  • Cell proliferation was determined by trypan blue dye exclusion test. (biomedcentral.com)
  • Thereafter, the downstream signaling pathways are activated promoting cell proliferation and/or survival. (biomedcentral.com)
  • We showed that overexpression of DKK3 can decrease cell invasion, proliferation, and colony forming ability of GBC cells. (amrita.edu)
  • Our data indicate that extracellular ST6GAL1 from remote sources can compensate for cellular ST6GAL1-mediated aggressive tumor cell proliferation and invasive behavior and has great clinical potential for extracellular ST6GAL1 as these molecules are in the extracellular space should be easily accessible targets. (nature.com)
  • 1991. Promotion of mouse lung tumors by bioaccumulated polychlorinated aromatic hydrocarbons. (cdc.gov)
  • Other partners of the intracellular metabolism of gemcitabine in relation to the cell cycle effects and DNA repair could be more responsible for the radiosensitising effect than dCK activity. (biomedcentral.com)
  • Cell death caused by PDT (through the generation of reactive oxygen species) can occur either by apoptosis (interphase death or as a secondary event following mitosis) and/or necrosis depending on the cell type, concentration and intracellular localization of the sensitizer, and the light dose. (elsevierpure.com)
  • Canonically, functioning ST6GAL1 resides within the intracellular ER-Golgi secretory complex, where sialylation of nascent cell surface and secreted components occurs cell-autonomously. (nature.com)
  • Very early during mammalian development the embryo differentiates into 2 main cell lineages, the inner cell mass (ICM) which will mainly contribute to embryonic tissues, and the trophoblast which will form extra-embryonic tissues. (biomedicineandprevention.com)
  • RASSF1A methylation was tumor specific and was absent in adjacent non-malignant tissues. (elsevierpure.com)
  • E-cadherin helps neighboring cells stick to one another (cell adhesion) to form organized tissues. (medlineplus.gov)
  • C. Peterson, M. Ringne´r / Artificial Intelligence in Medicine 28 (2003) 59-74 structure and function, cellular metabolism, development of cells and tissues, and response of organisms to their environments. (lu.se)
  • These include gamma-knife stereotactic radiosurgery and brachytherapy (radioactive seeds or sources are placed in or near the tumor itself, giving a high radiation dose to the tumor while reducing the radiation exposure in the surrounding healthy tissues). (medscape.com)
  • X. laevis is the only amphibian where true lymphoid tumors have been discovered and cell lines have been obtained opening new avenues for tumor biology and tumor immunity. (rochester.edu)
  • Cell cycle analysis suggested activation of apoptosis, without arresting cell cycle progression. (nature.com)
  • PS-3-PDT induced a significantly higher level of apoptosis in BMG-1 cells as compared to 4451 cells. (elsevierpure.com)
  • Present results show that the higher sensitivity to PS-3-PDT in glioma cells arise on account of a higher level of apoptosis and suggest that induction of apoptosis is an important determinant of photodynamic sensitivity in certain cell types. (elsevierpure.com)
  • SK228-induced apoptosis was confirmed by terminal deoxynucleotidyl transferase dUTP nick end labeling assay and immunohistochemistry of cleaved caspase-3 in tumors from treated mice. (ntnu.edu.tw)
  • In further examination, we found that the ATRA-induced apoptosis in GIST-T1 cells was accompanied by the down-regulated expression of survivin and up-regulated expression of Bax protein. (biomedcentral.com)
  • We found that NF-κB activity increased at one hour after TRAIL treatment in PC3-TR and LNCaP cells which Oglemilast are both resistant to TRAIL-induced apoptosis. (ecologicalsgardens.com)
  • In contrast NF-κB activity in LNCaP Oglemilast cells which are sensitive to TNFα-induced apoptosis and have very low basal NF-κB activity 24 25 was maintained at the same level even at 48 hours after treatment (Fig. 2B). (ecologicalsgardens.com)
  • Given our prior findings that this AP-1 family members play a critical role in regulating TRAIL-induced apoptosis in prostate malignancy cells 18 19 we wished to evaluate the role of AP-1 family members in regulating both TRAIL-induced and TNFα-induced apoptosis. (ecologicalsgardens.com)
  • Desk 1 summarizes our preliminary findings which shows that either down-regulation of NF-κB and/or upregulation of AP-1 actions are important elements for cancers cells to become delicate to TNFα-induced or TRAIL-induced apoptosis. (ecologicalsgardens.com)
  • Doxorubicin- and platinum-based agents are most commonly used with the platinum-based agents that induce apoptosis versus the doxorubicin-based drugs that promote cell arrest. (medscape.com)
  • A new form of 5-fluorouracil called fluoropyrimidine that has increased activity against malignant cells and while having fewer gastrointestinal side effects has shown to be efficacious against malignant salivary cancers and to potentiate the effects of radiotherapy by increasing apoptosis. (medscape.com)
  • ICE molecules are made to boost the activity of innate antitumor immune cells. (onclive.com)
  • Among all the studied palladium(II) complexes, the [Pd(TSC3)2] (8) complex exhibited high antitumor activity on the DU145 prostate carcinoma and K562 chronic myelogenous leukemia cells, with low values of the inhibitory concentration (0.01 and 0.02 𝜇M, resp. (edu.pe)
  • Tumor growth was measured every 2 to 4 days. (genoway.com)
  • Therefore the differences in cellular and subcellular morphology cell growth/invasion ability cytogenetics cell markers and expression pattern. (biopaqc.com)
  • The loss of this protein prevents it from acting as a tumor suppressor, contributing to the uncontrollable growth and division of cells . (medlineplus.gov)
  • These cells form tumors in syngeneic models, upon subcutaneous and systemic (i.v.) injection, in immunocompetent mice. (genoway.com)
  • Humanized CD3 C57/Bl6 mice were injected (s.c.) with 1x10 6 EL4-hCD19-LZ cells. (genoway.com)
  • Both cell lines had strong tumorigenicity in athymic nude mice. (biopaqc.com)
  • Orthotopic transplantation of NRCC generated tumor in every mice also. (biopaqc.com)
  • ICE is a bispecific engager of CD16A+ natural killer (NK) cells and macrophages and tumor antigens. (onclive.com)
  • The K3 cells expressed surface antigens and pluripotent genes similar to those of rBM-MSCs and presented tumor cell features. (oncotarget.com)
  • This observation and the expression of the major melanoma-associated antigens were all indicative of the melanocytic origin of MST-1 tumour. (tmu.edu.tw)
  • only partially sensitized resistant malignancy cells to proapoptotic effects of TNFα or TRAIL. (ecologicalsgardens.com)
  • However concomitant repression of NF-κB and activation of c-Fos/AP-1 significantly enhanced the proapoptotic effects of TNFα and TRAIL in resistant prostate malignancy cells. (ecologicalsgardens.com)
  • Physique 2 Alterations in NF-κB and AP-1 activities correlate with sensitivity Oglemilast to TRAIL and TNFα in prostate malignancy cells. (ecologicalsgardens.com)
  • To do so, monocyte-differentiated macrophages from healthy donor peripheral blood mononuclear cells were co-cultured for 4 hours with CMFDA-labeled, wild-type EGFR-expressing or KRAS -mutant tumor cells in the presence of 10 µg/mL of AFM24 or cetuximab. (onclive.com)
  • Importantly, transfection of primary tumor cells produced immunostimulatory levels of IL-2 as determined by increased thymidine incorporation by autologous peripheral blood mononuclear cells and lymphokine-activated killer cell activity. (arizona.edu)
  • Multiple solid tumor types were successfully transfected, but normal blood mononuclear cells and leukemic blasts were resistant to transfection. (arizona.edu)
  • The establishment and characterisation of paired autologous tumour cell line (MST-1) and tumour-infiltrating lymphocyte (TIL) culture from a tumour mass of a 14-year-old Taiwanese girl with soft tissue melanoma are described. (tmu.edu.tw)
  • Interleukin-2 (IL-2) expanded TILs had the predominant CD8 + phenotype and the capacity to lyse cells of the cultured autologous tumour. (tmu.edu.tw)
  • We compared microRNA expression patterns in three cisplatin resistant sublines derived from paternal cisplatin sensitive germ cell tumor cell lines in order to improve our understanding of the mechanisms of cisplatin resistance. (biomedcentral.com)
  • Three cisplatin resistant sublines (NTERA-2-R, NCCIT-R, 2102EP-R) showing 2.7-11.3-fold increase in drug resistance after intermittent exposure to increasing doses of cisplatin were compared to their parental counterparts, three well established relatively cisplatin sensitive germ cell tumor cell lines (NTERA-2, NCCIT, 2102EP). (biomedcentral.com)
  • Moreover, we describe for the first time an association of the up-regulation of micro-RNA species such as hsa-miR-512-3p/-515/-517/-518/-525 and down-regulation of hsa-miR-99a/-100/-145 with a cisplatin resistant phenotype in human germ cell tumors. (biomedcentral.com)
  • The paternal cell lines are (1) the p53-wt (wild type) pluripotent gonadal germ cell tumor cell line NTERA-2, (2) the p53-mutated pluripotent extragonadal nonseminomatous germ cell tumor cell line NCCIT and (3) the p53-wt nullipotent embryonal cell carcinoma 2102EP, derived from a primary human testicular teratocarcinoma. (biomedcentral.com)
  • Our approach was designed to characterize the role of micro-RNAs on the presumably multifactorial phenomenon of acquired cisplatin resistance in germ cell tumors. (biomedcentral.com)
  • Background/Aim: Yolk sac tumour (YST) is a rare malignant ovarian germ cell tumour that often occurs in young women or adolescents and exhibits an unfavourable outcome. (elsevierpure.com)
  • abstract = "Background: The current method for cell line authentication is genotyping based on short tandem repeat (STR)-PCR involving coamplification of a panel of STR loci by multiplex PCR and downstream fragment length analysis (FLA), usually performed by capillary electrophoresis. (port.ac.uk)
  • The degree of radiosensitisation was also cell line dependent and seemed to correlate with the sensitivity of the cell line to the cytotoxic effect of dFdC. (biomedcentral.com)
  • Importantly, some of these differences in sensitivity were in concordance with high expression of the targets or activation of the corresponding pathways in primary tumor samples of the same subtype. (tudelft.nl)
  • We observed low expression of DKK3 in gallbladder adenocarcinoma tumors and highly invasive GBC cell lines. (amrita.edu)
  • The sialyltransferase ST6GAL1 that adds α2-6 linked sialic acids to N-glycans of cell surface and secreted glycoproteins is prominently associated with many human cancers. (nature.com)
  • In general the expression of the most genes at 24?h culture wasn't significant different between MRCC and NRCC cells except that this expression of was significantly higher in MRCC cells than in NRCC cells (and was not significantly different between MRCC and NRCC cells. (biopaqc.com)
  • Aberrant promoter methylation of tumor suppressor genes has not been fully investigated in pediatric tumors. (elsevierpure.com)
  • Therefore, we examined the methylation status of nine genes (p16 INK4A , MGMT, GSTP1, RASSF1A, APC, DAPK, RARβ, CDH1 and CDH13) in 175 primary pediatric tumors and 23 tumor cell lines using methylation-specific PCR. (elsevierpure.com)
  • 2 3 Tumors with low copy number changes have generally been associated with high immune infiltration scores, 4 but for CNA-silent versus CNA-high HPV-negative HNSCC such data are lacking.In this study we aim to unravel by functional assays immunological differences between HPV-negative and HPV-positive HNSCC, as well as between CNA-silent and CNA-high HPV-negative HNSCC. (bmj.com)
  • We did simultaneous introduction of two tumor suppressor peptides (p14 ARF and p16 INK4a or p16 INK4a and p21 CIP1 functional peptides) compared with single-peptide introduction using Wr-T-mediated peptide delivery. (aacrjournals.org)
  • For the primary transplanted embryonic line, high toxicity was revealed when BNCT was performed with borphenylalanine and borcaptate. (isnct.net)
  • Carefully planned and executed surgical excision is the primary treatment for all primary salivary gland tumors. (medscape.com)
  • Adenocarcinomas develop in the glandular cells of the lining of the small intestine. (msdmanuals.com)
  • Using paclitaxel (Taxol), another antimicrotubule drug, alone has had moderate activity against mucoepidermoid tumors and adenocarcinomas but no effect adenoid cystic carcinoma. (medscape.com)
  • Methods We applied a new unsupervised, iterative approach to stratify CRC tumor samples into subtypes based on genome-wide mRNA expression data. (tudelft.nl)
  • Methods Eight HNSCC cell lines (3 HPV-positive, 3 HPV-negative CNA-high, 2 HPV-negative CNA-silent) and 24-hour supernatants of thirteen HNSCC biopsies were used to characterize their ability to attract immune cells in a transwell migration system. (bmj.com)
  • Materials and Methods: We established a novel YST cell line, TC587, from an adolescent patient with ovarian YST. (elsevierpure.com)
  • The innate cell engager AFM24 showed greater efficacy in eliciting antibody-dependent cellular phagocytosis of EGFR wild-type and KRAS-mutant tumor cells compared with cetuximab. (onclive.com)
  • The innate cell engager ® (ICE) AFM24 showed greater efficacy in eliciting antibody-dependent cellular phagocytosis of EGFR wild-type and KRAS -mutant tumor cells compared with cetuximab (Erbitux), according to preclinical findings from a study presented in a poster during the 2021 SITC Annual Meeting . (onclive.com)
  • AFM24 enhances macrophage-mediated antibody-dependent cellular phagocytosis of various EGFR-expressing tumor cell lines, irrespective of the EGFR signaling pathway," wrote lead study author Sheena Pinto, PhD, translational project leader at Affimed, and coauthors, in the poster. (onclive.com)
  • In this analysis, investigators evaluated the ability of AFM24 to elicit antibody-dependent cellular phagocytosis in solid tumor cell lines expressing EGFR wild-type or EGFR signaling pathway mutations. (onclive.com)
  • Additional results showed that AFM24 increased antibody-dependent cellular phagocytosis of EGFR -positive tumor cells regardless of macrophage subtype. (onclive.com)
  • Moreover, AFM24 was superior to cetuximab in eliciting phagocytosis in EGFR -positive cells in live cell imaging analysis over 24 hours. (onclive.com)
  • Pharmacological response data for targeted inhibitors of SRC, WNT, GSK3b, aurora kinase, PI3 kinase, and mTOR, showed significant differences in sensitivity across cell lines assigned to different subtypes. (tudelft.nl)
  • The noted lack of CD8+ T-cell attraction may explain why current treatments with PD-1 inhibitors are effective in only a minority of HNSCC patients. (bmj.com)
  • To tailor CRC treatment, it is essential to first model this heterogeneity by defining subtypes of patients with homogeneous biological and clinical characteristics and second match these subtypes to cell lines for which extensive pharmacological data is available, thus linking targeted therapies to patients most likely to respond to treatment. (tudelft.nl)
  • Results: The cell line expressed AFP and SALL4, the characteristics of YST. (elsevierpure.com)
  • This is often combined with postoperative radiation therapy, depending on the specific tumor characteristics and stage. (medscape.com)
  • However, AFM24 engages CD16A on NK cells and macrophages with a higher affinity than monoclonal antibodies, causing NK cell-mediated antibody-dependent cellular cytotoxicity and macrophage-mediated antibody-dependent cellular phagocytosis. (onclive.com)
  • The hsa-miR-371-373 cluster (counteracting cellular senescence and linked with differentiation potency), as well as hsa-miR-520c/-520h (inhibiting the tumor suppressor p21) were 3.9-16.3 fold up-regulated in two of the three cisplatin resistant cell lines. (biomedcentral.com)
  • An early increase of local cytotoxic cells correlates with GET treatment and an increase of long-term memory T cells results from animals with complete tumor regression. (frontiersin.org)
  • Tumor aneuploidy correlates with markers of immune evasion and with reduced response to immunotherapy. (bmj.com)
  • The microfluidic electrophoresis method produced highly reproducible results with good sensitivity in sizing of multiple PCR fragments, and each cell line demonstrated a unique DNA profile. (port.ac.uk)
  • Physique 1 Prostate malignancy cell lines PC3 PC3-TR and LNCaP have differential sensitivity to TRAIL and TNFα. (ecologicalsgardens.com)
  • EL4-hCD19-LZ cells form tumors in vivo when injected subcutaneously. (genoway.com)
  • IL-15cx shows superior in vivo bioactivity to expand CD8 T cells in comparison to an equimolar single chain IL-15. (frontiersin.org)
  • T-bet is partially involved in CD8 T cell expansion ex vivo and in vivo due to IL-15 or IL-15cx. (frontiersin.org)
  • However, all these different cell lines do express non-classical MHC class Ib (XNC) and b2-microglobulin mRNA. (rochester.edu)
  • We treated six cell lines lacking RASSF1A mRNA with 5-aza-2′deoxycytidine to examine the relationship between methylation and transcriptional silencing. (elsevierpure.com)
  • In five of six cell lines, restoration of RASSF1A mRNA was confirmed by RT-PCR. (elsevierpure.com)
  • Besides, administration of concentrated extracts of Morinda citrifolia in tumor containing animals increased the immune response of the animals with a concomitant reduction in tumor burden 7 . (nature.com)
  • Established tumor cell lines produced 10- to 100-fold more IL-2 than did fresh or short-term tumor cultures as measured by enzyme-linked immunoabsorbent analysis. (arizona.edu)
  • Imatinib, a selective tyrosine kinase inhibitor, has been used as a standard first-line therapy for irresectable and metastasized gastrointestinal stromal tumor (GIST) patients. (biomedcentral.com)
  • Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal neoplasms occurring throughout the entire region of the gastrointestinal tract and are considered to originate from intestitial cells of Cajal, the pacemaker cells of the gut [ 1 ]. (biomedcentral.com)
  • Some gastrointestinal neuroendocrine tumors can be removed surgically. (msdmanuals.com)
  • Results Most HNSCC cell lines induced migration of monocytes, B cells and CD4+ T-cells up to maximal 12%, whereas CD8+ T-cells and conventional dendritic cells (cDCs) were not attracted, irrespective of the donor. (bmj.com)
  • Tumor-native ST6GAL1 promotes tumor cell behaviors such as invasion and resistance to cell stress and chemo- and radio-treatments. (nature.com)
  • This protein is found within the membrane that surrounds epithelial cells, which are the cells that line the surfaces and cavities of the body. (medlineplus.gov)
  • Mesodermal reinforcement along lines of fusion is important, as epithelial breakdown and clefting is thought to result from the lack of reinforcement. (medscape.com)