Lineages progenitor satellite cell. Medical search. Frequent questions

Skeletal muscle, a known and plentiful source of progenitor cells is commonly associated with extraskeletal pathologies such as muscular dystrophy and heterotopic ossification. (uconn.edu)
Part I will be confined to the importance of source of progenitor cells, whether adult stem cells or umbilical cord blood. (pharmaceuticalintelligence.com)

Oligodendrocyte formation in the adult brain is associated with glial-restricted progenitor cells, known as oligodendrocyte progenitor cells (OPCs). (wikipedia.org)
Intro Satellite cells are the cells specific stem cells in the adult skeletal muscle mass. (healthyguide.info)
Interestingly recent data indicated that although MyoD is not indicated in quiescent satellite cells in the adult it is transiently indicated in satellite cell progenitors in the embryo suggesting that satellite cells are derived from committed embryonic precursors of myogenic lineage [4] [5]. (healthyguide.info)
In this regard, we have concentrated on the tissue resident adult progenitors in skeletal muscle and specifically, their role in soft tissue associated heterotopic ossification. (uconn.edu)
Single-cell comparison to human fetal and adult myogenic progenitor /satellite cells reveals distinct molecular signatures for non-dividing myogenic progenitors in activated (CD44High/CD98+/MYOD1+) and dormant (PAX7High/FBN1High/SPRY1High) states. (bvsalud.org)
This can be achieved using induced pluripotent stem cells (iPSCs), human adult cells that have been 'reprogrammed' to behave like cells in the embryo that can develop in to almost any cell in the body. (bvsalud.org)
Satellite television cells (SCs) sustain muscle development and empower adult skeletal muscle with strenuous regenerative capabilities. (cancer-ecosystem.com)
In Drosophila , a population of muscle-committed stem-like cells called adult muscle precursors (AMPs) keeps an undifferentiated and quiescent state during embryonic life. (biologists.com)
The embryonic AMPs are at the origin of all adult fly muscles and, as we demonstrate here, they express repressors of myogenic differentiation and targets of the Notch pathway known to be involved in muscle cell stemness. (biologists.com)
Together with other published results, these observations blur the concept of fixed cell types and provide new insights concerning potential uses for adult stem cells. (innovations-report.com)
In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice. (biomedcentral.com)
More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)
In contrast to adult cancers and previous NB studies, we show an increase in inflammatory monocyte cell state when contrasting normal and tumor tissue, while no differences in cytotoxicity and exhaustion score for T cells, nor in Treg activity, are observed. (pklab.org)
Laminin-521 also has properties that improve the isolation and expansion of adult stem cells and progenitors. (biolamina.com)
Adult stem cells play key roles in homeostasis and tissue repair. (biomed.news)

In regenerative biology, the overarching goal is to rebuild degenerating or absent tissue with a patient's own cells. (uconn.edu)
While this ultimate aim is rather ambitious, the motivation to achieve these ideal clinical tissues has driven the field of stem and progenitor cell biology to unprecedented levels of scientific and public involvement. (uconn.edu)
The most prominent example is the changes in cell biology and morphology when it evolves from a healthy to a cancerous state [1, 3]. (globaltechbiz.com)
2009 potentially reflecting the reduced secretion of myogenic growth element IGF-1 by these cells (Wynes and Riches 2003 While these studies demonstrate a facilitative part of innate immune cells in muscle mass regrowth after injury a direct molecular link between the innate immune system and muscle mass progenitor biology remains to be founded. (scienceexhibitions.org)
Findings The present research exposed that EZH2 affects many elements of South carolina biology, including self-renewal, expansion, and cell identification. (cancer-ecosystem.com)
Scientists at the University of Pennsylvania School of Veterinary Medicine have found a new wrinkle in the developmental biology dogma that cell differentiation occurs irreversibly as stem cells give rise to increasingly specialized types of offspring cells. (innovations-report.com)
Insights into the biology of stem cells will be gained by confirmation and characterization of candidate MaSC markers identified in this study. (frontiersin.org)
The DKFZ is a multidisciplinary center covering cancer biology from basic molecular and cell biology to clinical research and care. (mdc-berlin.de)
Despite successes in pre-clinical animal models with stem cells, a problem arises with respect to the biology of the transplanted progenitor cells. (pharmaceuticalintelligence.com)
4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)

In vitro culture systems that structurally model human myogenesis and promote PAX7+ myogenic progenitor maturation have not been established. (bvsalud.org)
Embryonic myogenic progenitor cells express CXCR4, G-protein coupled cell surface receptor, and migrate towards regions of SDF-1 expression during limb muscle development, suggesting that CXCR4/SDF-1 plays a role in muscle cell homing. (biomedcentral.com)

Furthermore, we decisively prove through in vitro clonal assays that the majority of these mesenchymal progenitors are capable of multilineage differentiation, contributing to spontaneously forming adipogenic and fibrocyte-like cells, and to BMP-induced osteogenic cells. (uconn.edu)
Here we show that human PSM cells derived in vitro-as well as those of the mouse 4 -recapitulate the oscillations of the segmentation clock. (nature.com)
Single-cell RNA sequencing reveals that mouse and human PSM cells in vitro follow a developmental trajectory similar to that of mouse PSM in vivo. (nature.com)
Fig. 1: Recapitulation of the mouse and human segmentation clocks in vitro by differentiation of pluripotent stem cells towards PSM fate. (nature.com)
Our experiments will use in vitro cell culture including activated caspase and stress-induced death assays. (umn.edu)
To assess the differentiation capabilities of these cells, we will use flow cytometry to sort the CD34+ population, followed by in vitro differentiation assays using various growth factors and serum deprivation. (umn.edu)
In vitro, conditioned medium from exo-adipocytes promoted HepG2 cell migration and increased tube formation of human umbilical vein endothelial cells (HUVECs). (biomedcentral.com)
This unique duality makes them an attractive system for potential regenerative medicine and cell therapies, but also for differentiation studies in vitro and for modeling diseases. (biomedcentral.com)
Previous molecular characterizations of mammary stem cells (MaSC) have utilized fluorescence-activated cell sorting or in vitro cultivation of cells from enzymatically dissociated tissue to enrich for MaSC. (frontiersin.org)
Because stromal cells can modulate the functionality of myeloid cells in vitro, targeting stromal-myeloid interactions has become an attractive potential therapeutic strategy. (pklab.org)
Skoglund G, Lainé J, Darabi R , Fournier E, Perlingeiro R, Tabti N. Physiological and ultrastructural features of human induced pluripotent and embryonic stem cell-derived skeletal myocytes in vitro. (uh.edu)
A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)

Analysis of supporting stromal cells within the tumor environment demonstrates vascular remodeling within the endothelial cells. (pklab.org)
6- Differentiation of iPSCs into cardiomyocytes and endothelial cells for disease modeling and screening. (uh.edu)

Muscle satellite cells make up a stem cell populace that is capable of differentiating into myocytes and contributing to muscle mass regeneration upon injury. (healthyguide.info)
Here we report that human skeletal muscle organoids can be differentiated from induced pluripotent stem cell lines to contain paraxial mesoderm and neuromesodermal progenitors and develop into organized structures reassembling neural plate border and dermomyotome. (bvsalud.org)
These results indicate that the Wnt signaling pathway may play a critical role in tissue-specific stem cell aging and an increase in tissue fibrosis with age. (elsevierpure.com)
KIT is a receptor tyrosine kinase type III, which binds to stem cell factor. (inhibitorkit.com)
There his work focused on transcriptional control of hematopoietic stem cell maturation and cell fate. (ubc.ca)
In addition, he identified a number of novel hematopoietic stem cell surface proteins and began analyzing their function. (ubc.ca)
He has delineated the function of these molecules in diverse set of biological processes including: 1) gut and kidney formation, 2) vascular permeability, 3) mucosal inflammatory disease, 4) stem cell homing and migration, and 5) epithelial tumor progression. (ubc.ca)
He is a member of the Stem Cell Network Centre of Excellence (past member of the Stem Cell Policy Committee and Research Management Committee and current Sub-chair of the Training and Education Committee), and a member of the AllerGen Network Centre of Excellence (Research Management Committee and Co-Chair of the Biomarkers Program). (ubc.ca)
Enriched expression of genes in LRECb was associated with stem cell attributes and identified WNT, TGF-β, and MAPK pathways of self renewal and proliferation. (frontiersin.org)
whereas LREC in suprabasal epithelial layers are enriched for more committed progenitor cells, expressing some genes that are associated with stem cell attributes along with those indicative of cell differentiation. (frontiersin.org)
Retention of labeled DNA strands may be attributed to the ability of stem cells to retain the parental DNA strand during asymmetric cell division ( Cairns, 1975 ) or to quiescence of the stem cell population such that the DNA label is not diluted by frequent cell divisions ( Klein and Simons, 2011 ). (frontiersin.org)
During periods of low mammary proliferation, quiescence of the stem cell population may account for retention of label. (frontiersin.org)
Therefore, these results establish the efficacy of diprotin A in stimulating muscle cell engraftment, and highlight the pre-clinical utility of a xenotransplantation model in assessing the relative efficacy of muscle stem cell populations. (biomedcentral.com)
We used a clinically acceptable regimen of hematopoietic stem cell transplantation to establish mixed donor/host blood cell chimerism and immune tolerance in a canine model of Duchenne muscular dystrophy ( cxmd ) [ 9 ]. (biomedcentral.com)
The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
This series of articles discusses the difficulties we have encountered in adopting stem cell research to clinical therapeutics in regeneration of cardiac tissue damaged post myocardial infarct. (pharmaceuticalintelligence.com)
These include generation of knock-in reporter stem cell lines to study myogenic differentiation, CRISPR/Cas9 gene targeting methods to correct mutated genes in muscular dystrophies, and using bioscaffolds, seeded with stem cells, to treat skeletal muscle injuries. (uh.edu)
Wu J, Matthias N, Lo J, Ortiz-Vitali JL, Shieh AW, Wang SH, Darabi R . A Myogenic Double-Reporter Human Pluripotent Stem Cell Line Allows Prospective Isolation of Skeletal Muscle Progenitors. (uh.edu)
Wu J, Hunt SD, Matthias N, Servián-Morilla E, Lo J, Jafar-Nejad H, Paradas C, Darabi R . Generation of an induced pluripotent stem cell line (CSCRMi001-A) from a patient with a new type of limb-girdle muscular dystrophy (LGMD) due to a missense mutation in POGLUT1 (Rumi). (uh.edu)
Matthias N, Hunt SD, Wu J, Darabi R . Skeletal muscle perfusion and stem cell delivery in muscle disorders using intra-femoral artery canulation in mice. (uh.edu)
Assessment of the myogenic stem cell compartment following transplantation of pax3/pax7-induced embryonic stem cell-derived progenitors. (uh.edu)
Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
During stem cell cardiac differentiation, a distinct swish in the expression profile of laminin subunits has been reported with laminin-411/421 pre-dominantly expressed early in progenitors and laminin-211/221 expressed later in cardiomyocytes (Ja, 2015). (biolamina.com)
A highly reproducible, chemically defined, xeno-free laminin-based differentiation protocol to generate stem cell-derived cardiovascular progenitors (CVPs) was reported in 2019 (Yap, 2019). (biolamina.com)

They arise during development from oligodendrocyte precursor cells (OPCs), which can be identified by their expression of a number of antigens, including the ganglioside GD3, the NG2 chondroitin sulfate proteoglycan, and the platelet-derived growth factor-alpha receptor subunit (PDGF-alphaR). (wikipedia.org)
WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)
CD34 is expressed on early precursor cells of myeloid and B-cell lineages, and CD15 is expressed on immature monocytic lineage cells and highly expressed on granulocytic lineage cells.31 As diseased muscle contains many myeloid cells such as neutrophils, monocytes, and macrophages, a more specific marker for mesenchymal cells with adipogenic potential is AAF-CMK required for detailed characterization of these cells in human diseased muscle. (phytid.org)
We have also been able to show that there are a greater number of precursor cells within the EOM than limb. (umn.edu)
Myogenic precursor cells in craniofacial muscles. (umn.edu)
Rodents with conditional mutilation of Ezh2 in Pax7-produced muscle mass precursor cells possess decreased muscle mass mass with smaller sized myofibers was selectively ablated in Pax7-produced cells by traversing knock-in rodents articulating Cre recombinase from the Pax7 locus (alleles (Su et al. (cancer-ecosystem.com)

One such alternative cell fate is the adipogenic fate as Pax7(+) satellite cells isolated from solitary myofibers used adipogenic fate in addition ARHGAP1 to muscle mass fate significance of these factors we used a mouse fracture healing model inside a genetically altered reporter mouse where muscle mass progenitors were lineage-traced. (healthyguide.info)
Instead, we identify a novel mesenchymal cell type located in the interstitial space of skeletal muscle that harnesses robust in vivo chondrogenic, osteogenic and adipogenic capabilities. (uconn.edu)
CD56+ cells did not show any adipogenic activity, but a few CD56?PDGFRand and peroxisome proliferator-activated receptor-(PPARand PDGF signaling on PDGFRand PDGF signaling on AAF-CMK PDGFRphosphorylation is represented as the ratio to unstimulated control cells (cont) and shown as meanss.d. of three independent preparations. (phytid.org)
2008 In addition to satellite cells recent studies have recognized an important part for fibro/adipogenic progenitors (FAPs) in muscle mass regeneration and its fatty degeneration (Joe et al. (scienceexhibitions.org)

Here we show by in vivo fate mapping that brown, but not white, fat cells arise from precursors that express Myf5, a gene previously thought to be expressed only in the myogenic lineage. (bioseek.eu)
Critically, we demonstrate that Sox10 is expressed transiently in the sensory neuron lineage, and specifies sensory neuron precursors by regulating the proneural gene neurogenin1 . (silverchair.com)
Derivation of Skeletal Myogenic Precursors from Human Pluripotent Stem Cells Using Conditional Expression of PAX7. (uh.edu)

We isolated four categories of cells from mammary epithelium of female calves: bromodeoxyuridine label retaining epithelial cells (LREC) from basal (LRECb) and embedded layers (LRECe), and epithelial control cells from basal and embedded layers. (frontiersin.org)
This dictates cycles of mammary growth, differentiation, lactation, and regression, during which mammary stem cells (MaSC) provide for the lineages of luminal and basal (myoepithelial) epithelial cells in the ducts and alveoli. (frontiersin.org)
During rapid mammary growth in the mouse, label retaining epithelial cells (LREC) appear to retain label by asymmetric distribution of DNA strands, as evidenced by a rapid proliferation index of the LREC ( Smith, 2005 ). (frontiersin.org)

Myofiber-specific ablation of IRE1α or XBP1 in mice diminishes skeletal muscle regeneration that is accompanied with reduced number of satellite cells. (elifesciences.org)
Finally, targeted ablation of IRE1α also reduces Notch signaling, abundance of satellite cells, and skeletal muscle regeneration in the mdx mice, a model of Duchenne muscular dystrophy. (elifesciences.org)
Collectively, our experiments suggest that the IRE1α-mediated signaling promotes muscle regeneration through augmenting the proliferation of satellite cells in a cell non-autonomous manner. (elifesciences.org)
The findings shed new light on mechanisms of regeneration of healthy myofibers after severe tissue injury and suggest interplay between these fibers and muscle satellite cells though mechanisms remain to be elucidated. (elifesciences.org)
Remarkably type 2 cytokine signaling is also required in FAPs but not myeloid cells for quick clearance of necrotic debris a process that is necessary for timely and total regeneration of BV-6 cells. (scienceexhibitions.org)
In a number of species cells regeneration is associated with the presence of the molecular signature for type 2 innate immune response such as alternatively triggered (M2) macrophages and eosinophils (Allen and Wynn 2011 Palm et al. (scienceexhibitions.org)
In 2009 beginning of 2010 we have focused on investigating what factors human embryonic stem cells (hESCs) may produce that enhance regeneration and if those factors have any effects by themselves on regeneration. (ca.gov)
Finally, our data suggest that muscle stem cells either do not accumulate DNA damage with age or can efficiently repair such damage, when activated for tissue regeneration. (ca.gov)
This is Part 3 of a series of contributions on cardiac regeneration after myocardial infarct with stem cells. (pharmaceuticalintelligence.com)
Part I went into some detail on a method of obtaining suitable cells, growing them in sheets, and transferring the sheets to the surface for regeneration and repair, which is now going into clinical trials. (pharmaceuticalintelligence.com)
The main interest of Dr. Darabi's lab is to use pluripotent stem cells for skeletal muscle regeneration in muscular dystrophies and muscle injuries. (uh.edu)

Culture conditions instigate neural lineage arrest and promote fetal hypaxial myogenesis toward limb axial anatomical identity, with generation of sustainable uncommitted PAX7 myogenic progenitors and fibroadipogenic (PDGFRa+) progenitor populations equivalent to those from the second trimester of human gestation. (bvsalud.org)
mutilation in Pax7-produced skeletal muscle mass AZD8330 manufacture cells outcomes in problems of postnatal muscle mass development characterized by decreased muscle mass mass with smaller sized muscle mass materials. (cancer-ecosystem.com)
The decreased quantity of Pax7+ cells in = 2) 3 m after CTX, probed with myogenin, Pax7, and GAPDH antibodies. (cancer-ecosystem.com)
Wu J, Hunt SD, Xue H, Liu Y, Darabi R . Generation and Validation of PAX7 Reporter Lines from Human iPS Cells Using CRISPR/Cas9 Technology. (uh.edu)
Laminin-521 supports increased differentiation potential without altering the traditional Pax7/MyoD paradigm and the results are translational across several mouse backgrounds, human cells, and disease states. (biolamina.com)

Both miRNAs and siRNAs need to be delivered into the target tissue or cell in order to activate the desired therapeutic effect," Muthiah Manoharan, Ph.D., senior vp, drug discovery, Alnylam Pharmaceuticals, explained. (genengnews.com)
Here, we focus on identifying and characterizing tissue resident progenitors. (uconn.edu)
Skeletal muscle, the most abundant tissue of the body, has remarkable regenerative capacity mainly due to its resident muscle stem cells, also known as satellite cells. (elifesciences.org)
Our work is at the stage of understanding the molecular mechanisms by which the aging of the regenerative potential of organ stem cells can be reversed by particular human embryonic factors that are capable of neutralizing the affects of aged niches on tissue regenerative capacity. (ca.gov)
Thus, the use of hESC-produced pro-regenerative factors for boosting the regenerative capacity of organ stem cells is likely to yield healthy, young tissue. (ca.gov)
Although functional organ stem cells persist in the old, tissue damage invariably overwhelms tissue repair, ultimately causing the demise of an organism. (ca.gov)
How is the tissue specificity of immune cells generated and how is it affected by environmental influences, plasticity and the tissue microenvironment? (mdc-berlin.de)
These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
Enormous problems have been encountered in the selection of progenitor cells, the growth into compatible and functional myocardial tissue, and the survival of the myocardium. (pharmaceuticalintelligence.com)
We provide a comprehensive immune cell landscape and characterize cell-state changes from normal tissue to NB. (pklab.org)
A beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion in mice. (omicsdi.org)
The results support the concept that fuel excess can drive obesity and diabetes via hyperinsulinaemia, and that an islet beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion. (omicsdi.org)
The compromised tissue repair program is attributable to the gradual depletion of stem cells and to altered regulatory signals. (biomed.news)

Initially, it was originally thought that these cells were produced in the ventral neural tube, the embryonic precursor to the CNS. (wikipedia.org)
Satellite cells from your trunk and the limb are derived from an embryonic populace of progenitor cells in the somites transient mesodermal constructions that develop on either part of the neural tube [1]. (healthyguide.info)
sox10 is necessary for development of neural and pigment cell derivatives of the neural crest (NC). (silverchair.com)

Oligodendrocytes are a type of glial cell. (wikipedia.org)
Oligodendrocytes interact closely with nerve cells and provide trophic support by the production of glial cell line-derived neurotrophic factor (GDNF), brain-derived neurotrophic factor (BDNF), or insulin-like growth factor-1 (IGF-1). (wikipedia.org)
However, whereas a direct role for Sox10 activity has been established in pigment and glial lineages, this is more controversial in NC-derived sensory neurons of the dorsal root ganglia (DRGs). (silverchair.com)

We use in parallel many technologies that have been considerably refined to identify, isolate, and characterize, specific and practically pure cell populations, critically allowing us to attribute functional outcomes of potency tests to unique cell types. (uconn.edu)
Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)

There he worked with Dr. Max D. Cooper (Howard Hughes Medical Institute, National Academy of Sciences) and his research focused on cell surface proteins expressed by preB cells that regulate B cell maturation and homing. (ubc.ca)

The unit can offer higher-throughput systems than typical technologies such as for example atomic drive microscopy and micropipette aspiration [5] and will be used like a comparative tool between different subpopulations of cells. (globaltechbiz.com)
Single-cell RNA sequencing of CVPs derived from hESC lines identified three main progenitor subpopulations. (biolamina.com)

We use extensively, the Cre/loxP lineage tracing system to label putative plastic cell types and assess the inherent plasticity of these cells in biologically relevant mouse-chick chimeric models of developmental potency and bioassays of heterotopic ossification. (uconn.edu)
Two groups of resident progenitors, the skeletal muscle satellite cells (SCs) and vascular endothelium (VE)/vascular endothelial progenitors (VEPs), have been considered to be putative cell sources for ectopic lesions of heterotopic ossification due to their reported multipotent characteristics. (uconn.edu)
In conclusion, these data decisively establish degrees of potency for skeletal muscle resident progenitors and identify the putative cell-of-origin for the ectopic skeletal anlagen of heterotopic ossification. (uconn.edu)
Instead, we used laser microdissection to excise putative progenitor cells and control cells from their in situ locations in cryosections and characterized the molecular properties of these cells. (frontiersin.org)

Pluripotent embryonic stem cells (ESCs) have the unique ability to differentiate into every cell type and to self-renew. (biomedcentral.com)
Embryonic stem cells (ESCs), derived from the blastocyst-stage embryo, are capable of generating all cell types of the mammalian body (pluripotency) and of maintaining the capacity for indefinite self-renewal without compromising their genomic integrity. (biomedcentral.com)
Laminin-221 (LN221) was identified as the most likely expressed cardiac laminin and the authors show that this protein promotes differentiation of pluripotent human embryonic stem cells (hESCs) toward cardiomyocyte lineage and downregulates pluripotency and teratoma-associated genes. (biolamina.com)

Upon activation satellite cells rapidly initiate MyoD expression which leads to the activation of myogenin and terminally differentiated structural muscle mass genes such as myosin heavy chain (MHC) [1] [3]. (healthyguide.info)
LCGbase: A Comprehensive Database for Lineage-Based Co-regulated Genes. (openwetware.org)

These primitive-like cells hold promise as an autologous cell source for regenerative treatments and importantly, afford us the opportunity to begin to understand seemingly normal cellular processes in pathological contexts. (uconn.edu)
Ultimately, we hope to transplant our EOM-derived progenitor cells into injured limb muscle of mdx mice in order to determine the regenerative capabilities in vivo . (umn.edu)
Intro The regenerative response of skeletal muscle mass to ICAM4 injury is dependent within the quiescent populace of skeletal muscle mass stem cells termed the satellite cells which reside beneath the basal lamina of each myofiber (Brack and Rando 2012 Wang and Rudnicki 2012 Upon injury these quiescent satellite cells become triggered and undergo proliferation providing rise to myogenic progenitors (MPs) that ultimately differentiate into mature myofibers. (scienceexhibitions.org)
The poor performance of stem cells in an aged organ, such as skeletal muscle, is caused by the changes in regulatory pathways such as Notch, MAPK and TGF‐β, where old differentiated tissues and blood circulation inhibit the regenerative performance of organ stem cells. (ca.gov)

We show that muscle stem cells (satellite cells) from aged mice tend to convert from a myogenic to a fibrogenic lineage as they begin to proliferate and that this conversion is mediated by factors in the systemic environment of the old animals. (elsevierpure.com)
Furthermore, components of serum from aged mice that bind to the Frizzled family of proteins, which are Wnt receptors, may account for the elevated Wnt signaling in aged cells. (elsevierpure.com)
These cells are also present in the EOM of mdx mice and mdx/utrophin -/- mice, models for DMD. (umn.edu)
We suspect that this phenomenon is not limited to B-cells and macrophages in mice. (innovations-report.com)
Indeed, CXCR4-positive muscle-derived side population (SP) cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice [ 10 ]. (biomedcentral.com)
During the last several years, his lab has developed novel methods for multi-lineage differentiation of pluripotent stem cells for cell therapy in mice models of muscular dystrophies and injuries. (uh.edu)
Human ES and iPS-Derived Myogenic Progenitors Restore Dystrophin and Improve Contractility upon Transplantation in Dystrophic Mice. (uh.edu)
To directly assess the role of beta cell lipolysis in insulin secretion and whole-body energy homeostasis, inducible beta cell-specific adipose triglyceride lipase (ATGL)-deficient (B-Atgl-KO) mice were studied under normal diet (ND) and high-fat diet (HFD) conditions. (omicsdi.org)
At 8 weeks of age, these mice were injected with tamoxifen to induce deletion of beta cell-specific Atgl (also known as Pnpla2), and the mice were fed an ND or HFD. (omicsdi.org)

Single-cell RNA sequencing (scRNA-seq) has the potential to investigate both genetic and nongenetic sources of tumor heterogeneity in a single assay. (pklab.org)
Here, we perform single-cell RNA sequencing (scRNA-seq) on 19 human NB samples coupled with multiplex immunohistochemistry, survival analysis, and comparison with normal fetal adrenal gland data. (pklab.org)

I will perform the following experiments: 1) examine the phenotype and differentiation potential of these cells, 2) determine the mechanism for their resiliency to injury, and 3) examine their ability to repair or regenerate muscle in vivo . (umn.edu)
This in accordance with recent data presented by researchers at Icagen Inc., demonstrating that the laminin-521 cell culture matrix maintains the differentiation potential of mouse and human satellite cell-derived myoblasts, even during long-term culture expansion (Penton, 2016). (biolamina.com)

In pluripotent and differentiated cells HP1β is differentially localized and differentially associated with chromatin. (biomedcentral.com)
Deletion of HP1β, but not HP1α, in ESCs provokes a loss of the morphological and proliferative characteristics of embryonic pluripotent cells, reduces expression of pluripotency factors and causes aberrant differentiation. (biomedcentral.com)
However, in differentiated cells, loss of HP1β has the opposite effect, perturbing maintenance of the differentiation state and facilitating reprogramming to an induced pluripotent state. (biomedcentral.com)
At the same time, the first three trials using cells derived from pluripotent cells have begun. (biomedcentral.com)

His laboratory has followed two primary interests: 1) the transcription factor networks that regulate fate determination in various cells that make blood, and 2) the cell surface proteins expressed by hematopoietic stem cells that and allow them to communicate with their microenvironment. (ubc.ca)
and those transplanted with autologous hematopoietic stem cells. (cancernetwork.com)

These cells, theoretically, would not require transfection of dystrophin, and could potentially provide an autologous source of cells for transplant therapies. (umn.edu)
Intramuscular injection of donor muscle-derived cells into chimeric cxmd recipients restored dystrophin expression for at least 24 weeks in the absence of post-transplant immunosuppression, indicating that cell transplantation may be a viable therapeutic option for muscular dystrophy. (biomedcentral.com)
Diprotin A stimulation of CXCR4, however, significantly increased the number of canine dystrophin-positive muscle fibers and canine-derived satellite cells by enhancing donor cell proliferation. (biomedcentral.com)

The researchers have shown that certain mouse cells retain an ability to oscillate between very distinct blood cell types - B-cells and macrophages - long after what has been commonly regarded as the point of no return. (innovations-report.com)
It s possible that by targeting this gene with drugs, we may be able to convert malignant B-cell lymphomas into much less harmful histiocytomas, tumors composed of relatively inactive macrophages. (innovations-report.com)
Previous research has demonstrated that when B-cells are transplanted from an animal into a petri dish, the cells sometimes change into macrophages. (innovations-report.com)
Moreover, the cells could continue oscillating between B-cells and macrophages indefinitely, as many times as they were relocated. (innovations-report.com)
B-cells arise from a lymphoid progenitor, while macrophages come from a myeloid progenitor. (innovations-report.com)

Defining the heterogeneity of skeletal muscle-derived side and main population cells isolated immediately ex vivo. (umn.edu)
Ex vivo cultures of myofiber explants demonstrate that ablation of IRE1α reduces the proliferative capacity of myofiber-associated satellite cells. (elifesciences.org)
These approaches result in the loss of all histological information pertaining to the in vivo locale of MaSC and progenitor cells. (frontiersin.org)
However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftment and stimulated sustained proliferation of donor cells in vivo . (biomedcentral.com)

However, both markers are expressed on many different cell types, including myeloid cells of hematopoietic lineage. (phytid.org)
Background & aims: Myeloid cells are key regulators of cirrhosis, a major cause of mortality worldwide. (pklab.org)

Therefore our and analyses suggest that the balance of Pax3 Nkx3.2 and Sox9 may act as a molecular Talniflumate switch during the chondrogenic differentiation of muscle mass progenitor cells which may be important for fracture healing. (healthyguide.info)
Collectively our data suggest that the balance of Nkx3.2 Sox9 and Pax3 can act as a molecular switch during the chondrogenic differentiation of satellite cells which may play an important part in the healing process experiments were normalized to GAPDH. (healthyguide.info)
Therefore, we aimed to exploit the tumor biological implication and the therapeutic vulnerabilities of a clinically relevant molecular PDAC subgroup characterized by SMAD4 deficiency and high expression of the Nuclear Factor of Activated T-cells (SMAD4-/-/NFATc1High). (bvsalud.org)
These changes take place on the molecular level impacting properties of specific the different parts of cell inner structure, but resulting in alterations in mechanical properties of the complete cell ultimately. (globaltechbiz.com)
Here, we explore the role of HCC-derived exosomes in the cellular and molecular conversion of adipocytes into tumor-promoting cells. (biomedcentral.com)
Through gene knockout studies he has shown that these molecules act as a type of molecular "Teflon" to make cells more mobile and invasive and also facilitate chemotaxis. (ubc.ca)
Our results support the use of DNA label retention to identify MaSC and also provide a molecular profile and novel candidate markers for these cells. (frontiersin.org)

We speculate that, in dedicated or differentiated cells, the chromatin framework at chosen genomic areas may become insensitive to epigenetic adjustments triggered by mutilation. (cancer-ecosystem.com)
A even more unsuspecting and plastic material chromatin environment, such as that of Sera cells or non-committed Pax7+/Myf5? (cancer-ecosystem.com)
The minor fraction of HP1β that is chromatin-bound in ESCs is enriched within exons, unlike the situation in differentiated cells, where it binds heterochromatic satellite repeats and chromocenters. (biomedcentral.com)

In this regard, his lab has identified a novel family of hematopoietic cell surface proteins, called the CD34 family, and shown that these are essential for a number of developmentally important processes. (ubc.ca)

This group of tracks shows data from A human cell atlas of fetal gene expression . (ucsc.edu)
The Fetal Cells subtrack contains the data organized by cell type, with RNA signals from all cells of a given type pooled and averaged into one bar for each cell type. (ucsc.edu)
The Fetal Lineage subtrack shows similar data, but with the cell types subdivided more finely and by organ. (ucsc.edu)
Additional bar chart subtracks pool the cell by other characteristics such as by sex ( Fetal Sex ), assay ( FetalAssay ), donor ( Fetal Donor ID ), experiment ( Fetal Exp ), organ ( Fetal Organ ), and reverse transcription group ( Fetal RT Group ). (ucsc.edu)

It is hypothesized that satellite oligodendrocytes (or perineuronal oligodendrocytes) are functionally distinct from other oligodendrocytes. (wikipedia.org)
Adipose tissues contain two distinct types of fat cells, white and brown. (bioseek.eu)
Transposon-Derived and Satellite-Derived Repetitive Sequences Play Distinct Functional Roles in Mammalian Intron Size Expansion. (openwetware.org)
Single-cell transcriptomic analysis of tumor cells from the primary tumors reveals a distinct transcriptional signature that is predictive of metastatic potential and patient survival. (pklab.org)

There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)

This is unlike the satellite cells of limb muscles, which remain quiescent unless injury is sustained. (umn.edu)
These cells from EOM are more resistant to apoptosis than similar cells from limb muscles. (umn.edu)
Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)

Examples include finding proteins indicating cardiac problems or looking for circulating tumour cells that can indicate the spread of cancer. (50webs.com)

2008 For instance autocrine Notch signaling regulates the activation and proliferation of satellite cells (Bjornson et al. (scienceexhibitions.org)

Additionally, many pathological ailments, such as cancer and heterotopic ossification, are believed to be the consequence of abnormal progenitor cell behavior. (uconn.edu)
We validated the model using experimental data and used it to quantify the effects of cell components on its behavior. (globaltechbiz.com)
The nucleus is the largest organelle among sub-cellular components, demonstrating solid-elastic behavior [4], and it is typically stiffer than the cell itself [5]. (globaltechbiz.com)
Tumor behavior is determined by not only the malignant potential of tumor cell itself but also the signals from its microenvironment. (biomedcentral.com)

These cells lay beneath the basement membrane of the muscle mass fiber and are usually mitotically quiescent [1]. (healthyguide.info)
For example, muscle stem cells (MuSCs), located beneath the basal lamina, exist in the quiescent state but can transition to an activated, proliferative state upon injury. (biomed.news)

Semaphorins are a wide family of extracellular regulatory signals with a multifaceted role in cell-cell communication. (biomed.news)

We hope to identify a new source of cells for use in myoblast transfer therapy. (umn.edu)
By describing a means for EOM sparing in DMD, we may advance our understanding of the disease and identify a new source of cells for myoblast transfer therapy. (umn.edu)
Taken together, these data indicate that PRDM16 specifies the brown fat lineage from a progenitor that expresses myoblast markers and is not involved in white adipogenesis. (bioseek.eu)

Thus, these markers in combination with basal lamina staining were useful for the identification of human satellite cells. (phytid.org)
These cells are located beneath the basal lamina of myofiber in a dormant state. (elifesciences.org)

This work reveals that seemingly committed cells have more plasticity than we had thought," said senior author Andrei Thomas-Tikhonenko, assistant professor of pathobiology at Penn. (innovations-report.com)

DLGP: A database for lineage-conserved and lineage-specific gene pairs in animal and plant genomes. (openwetware.org)
The MyoD gene was duplicated during the teleost whole genome duplication and, while a second MyoD gene ( MyoD2 ) was subsequently lost from the genomes of some lineages (including zebrafish), many fish lineages (including Alcolapia species) have retained both MyoD paralogues. (mdpi.com)

Between midgestation and term birth in human cerebral white matter, three successive stages of the classic human oligodendrocyte lineage are found: OPCs, immature oligodendrocytes (non-myelinating), and mature oligodendrocytes (myelinating). (wikipedia.org)

We also demonstrate that the transcriptional regulator PRDM16 (PRD1-BF1-RIZ1 homologous domain containing 16) controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells. (bioseek.eu)
Conversely, ectopic expression of PRDM16 in myoblasts induces their differentiation into brown fat cells. (bioseek.eu)

Therefore, we sought to translate these results to a clinically relevant canine-to-canine allogeneic transplant model for Duchenne muscular dystrophy (DMD) and determine if CXCR4 is important for donor cell engraftment. (biomedcentral.com)

identification of cells equivalent to these mesenchymal progenitors in humans has considerable clinical implication. (phytid.org)
Examples are CRISPR/Cas9-mediated mutagenesis, genetic mouse models, cell-fate-mapping, organoids and tumor explants, as well as GMP production of pharmaceuticals and many years of experience in conducting clinical studies. (mdc-berlin.de)
In recent years, clinical trials with stem cells have taken the emerging field in many new directions. (biomedcentral.com)
A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
We have chosen to concentrate on the emerging therapeutics that broadly involves a wide range of cell types in clinical trials registered on the National Institutes of Health's clinical trials web site. (biomedcentral.com)

Subventricular zone cells migrate away from germinal zones to populate both developing white and gray matter, where they differentiate and mature into myelin-forming oligodendrocytes. (wikipedia.org)
This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)

Finally we found that in an mouse model of fracture healing where muscle mass progenitor cells were lineage-traced Nkx3.2 and Sox9 are significantly upregulated while Pax3 is significantly downregulated in the muscle mass progenitor cells that give rise to chondrocytes during fracture restoration. (healthyguide.info)
Upon injury or when challenged with a variety of mechanical or biochemical stimuli satellite cells re-enter the cell cycle and give rise to Talniflumate differentiated myocytes which form new muscle mass materials or fuse with existing materials and contribute to muscle mass growth and restoration [1]. (healthyguide.info)
In the beginning satellite cells were considered to Talniflumate be unipotent stem cells with the ability of generating a unique specialized phenotype the skeletal muscle mass cells. (healthyguide.info)
We found that in the descendents of muscle mass progenitors that contributed to cartilage formation Nkx3.2 and Sox9 were strongly induced while Pax3 manifestation was strongly repressed. (healthyguide.info)
The iPSCs can then be converted into specific cell types in the laboratory, including the cells that make up skeletal muscle. (bvsalud.org)
created a protocol for developing iPSCs into three-dimensional organoids which resemble how cells of the skeletal muscle look and arrange themselves in the fetus. (bvsalud.org)
Several studies reported the identification of satellite cells in human skeletal muscle. (phytid.org)
We first identified satellite cells on human muscle sections. (phytid.org)
We observed 434 M-cadherin+ sublaminar satellite cells on muscle sections from 10 different patients, and found 99.5% of them were also positive for CD56 (Figure 1b). (phytid.org)
Specifically, the muscle progenitor cells, called satellite cells, are activated in the apparent absence of injury. (umn.edu)
Sparing of extraocular muscle in aging and muscular dystrophies: a myogenic precursor cell hypothesis.Exp Cell Res. (umn.edu)
The close association of FAPs with regenerating muscle mass fibers along with their manifestation of factors that influence myogenic differentiation such as IL-6 and IGF-1 suggests that these stromal cells may perform a supportive part in myogenic differentiation (Joe et BV-6 al. (scienceexhibitions.org)
2009). We produced rodents in AZD8330 manufacture which was conditionally ablated in SCs (muscle mass knockout, mouse, where cells that communicate or possess ever indicated Myf5-Cre are YFP+, while cells that possess by no means indicated Myf5-Cre are YFP? (cancer-ecosystem.com)
non-committed originate cells and Pax7+/Myf5+ muscle mass progenitors. (cancer-ecosystem.com)
Body and muscle mass mass had been significantly decreased in mutilation in skeletal myogenic cells. (cancer-ecosystem.com)
We confirmed that typical growth factor signaling was in fact occurring in muscle cells exposed to hESC produced factors, and that hESCs produce a TGF-beta antagonist. (ca.gov)
In the next reporting period we will re-confirm that the levels of candidate proteins from the 500 antibody array actually are very highly produced by hESCs and that the signals from these proteins are perceived by regenerating muscle cells. (ca.gov)
Transplantation of myogenic stem cells possesses great potential for long-term repair of dystrophic muscle. (biomedcentral.com)
In this study, we used a canine-to-murine xenotransplantation model to quantitatively compare canine muscle cell engraftment, and test the most effective cell population and modulating factor in a canine model of DMD using allogeneic transplantation experiments. (biomedcentral.com)
We show that CXCR4 expressing cells are important for donor muscle cell engraftment, yet FACS sorted CXCR4-positive cells display decreased engraftment efficiency. (biomedcentral.com)
Furthermore, the canine-to-murine xenotransplantation model accurately predicted results in canine-to-canine muscle cell transplantation. (biomedcentral.com)
Yet, it remains unclear from murine transplantation experiments which cell population most effectively engrafts into diseased skeletal muscle. (biomedcentral.com)
We used the xenotransplant model to show that CXCR4 expression on canine donor muscle cells is also important for cell engraftment, but FACS sorting for CXCR4-positive cells decreased their engraftment efficiency. (biomedcentral.com)
Matthias N, Hunt SD, Wu J, Lo J, Smith Callahan LA, Li Y, Huard J, Darabi R . Volumetric muscle loss injury repair using in situ fibrin gel cast seeded with muscle-derived stem cells (MDSCs). (uh.edu)

The epidemic of obesity, closely associated with increases in diabetes, hypertension, hyperlipidemia, cancer and other disorders, has propelled a major interest in adipose cells and tissues. (bioseek.eu)

Fracture Healing in Diabetes Fracture repair entails recruitment of mesenchymal stem cells after which differentiation to chondrocytes that type cartilage (see Chapter 12). (dnahelix.com)

Wu J, Hunt SD, Xue H, Liu Y, Darabi R . Generation and Characterization of a MYF5 Reporter Human iPS Cell Line Using CRISPR/Cas9 Mediated Homologous Recombination. (uh.edu)

These cells were isolated using CD3426, 30 or CD1527, 28 as markers. (phytid.org)
I have found a CD34+ group of cells in EOM that is negative for other lineage-specific markers. (umn.edu)

Previous studies have focused intensively on the interactions between HCC cells and a wide variety of immune cells such as Kupffer cells, NK cells, T cells, and several antigen-presenting cells. (biomedcentral.com)
Researchers are uncovering ever more details about the complex system of organs, tissues, and different cell types that make up the human immune system. (mdc-berlin.de)
One of the research areas at the MDC is immunology and inflammation, which covers tumor immunology, DNA repair in B lymphocytes, the role of tumor stroma-immune cell interaction in cancers, the role of innate immune cells in gliomas and neurodegenerative diseases, and the development of immunotherapy strategies. (mdc-berlin.de)
While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
Understanding the complete immune cell composition of human neuroblastoma (NB) is crucial for the development of immunotherapeutics. (pklab.org)

We demonstrate an unexpected duality in the role of HP1β: it is essential in ESCs for maintaining pluripotency, while it is required for proper differentiation in differentiated cells. (biomedcentral.com)

However, it is not clear whether all oligodendrocyte progenitors undergo this sequence of events. (wikipedia.org)

Anatomy62

Organisms25

Diseases8

Chemicals and Drugs75

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Phenomena and Processes52

Disciplines and Occupations1

Technology, Industry, Agriculture2

Information Science7

Health Care2

Source of progenitor cells2

Adult15

Biology10

Myogenic progenitor2

Vitro12

Endothelial cells2

Stem cell25

Precursor cells6

Adipogenic4

Precursors3

Epithelial3

Regeneration11

Pax75

Tissue14

Neural3

Glial3

Populations2

Maturation1

Subpopulations2

Putative4

Embryonic stem3

Genes2

Regenerative4

Mice9

Single-cell RNA2

Differentiation potential2

Pluripotent4

Hematopoietic stem2

Dystrophin3

Macrophages5

Vivo4

Myeloid cells2

Molecular7

Chromatin3

CD341

Fetal4

Distinct4

Autologous1

Limb3

Cardiac1

Proliferation of satellite cells1

Behavior4

Quiescent2

Extracellular1

Myoblast3

Basal2

Reveals1

Genomes2

Immature1

Myoblasts2

Transplant1

Clinical5

Differentiate2

Muscle24

Adipose1

Cartilage1

Characterization1

Markers2

Immune5

ESCs1

Undergo1