Lineage stem cell differentiation differentiation of cells. Medical search. Frequent questions

However, to our knowledge, there is no report on the successful generation of glucose-responsive insulin-producing beta-cells from hPSCs in vitro. (ca.gov)
The protocol, which spans five distinct stages, is an attempt to recapitulate the derivation of pancreatic beta-cells in vitro as they form in the developing embryo. (ca.gov)
Instead, they show a severely compromised capacity for successful differentiation towards neural or mesodermal fates and failed to correctly initiate lineage-specific gene expression in vitro. (ox.ac.uk)
These stem cells are capable of differentiating into chondrocytes, cardiomyocytes, melanocytes, and hepatocyte‐like cells in vitro. (wikipedia.org)
In their previous study, the researchers found that epimedin C could induce endothelial-like (but not osteogenic) differentiation of C3H/10T1/2 cells in vitro. (naturalnews.com)
Employing in vitro cell culture of inherited mitochondrial disease and an in vivo animal experimental model of low-grade inflammation (high fat feeding), we show human-derived MSCs to alter mitochondrial function. (frontiersin.org)
Skoglund G, Lainé J, Darabi R , Fournier E, Perlingeiro R, Tabti N. Physiological and ultrastructural features of human induced pluripotent and embryonic stem cell-derived skeletal myocytes in vitro. (uh.edu)
Directing differentiation of human pluripotent stem cells into different endodermal lineages such that we may generate tissue for replacement therapies and discover novel in vitro methods to model and study human development and disease. (aiche.org)
erythroid cells after in vitro differentiation, despite having similar frequencies of phenotypic HSC initially. (lu.se)
These HPLs can substitute for fetal bovine serum as a clinical grade xeno-free supplement of growth media used in the in vitro expansion of human cells for transplantation purposes. (bvsalud.org)
With advancements in stem cell technology, the possibility to generate astrocytes in vitro from human pluripotent stem cells (hPSCs), ultimately derived from patient cells, emerged. (lu.se)
Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)
The discovery of murine norovirus (MNV) in 2003 and the fact that this virus successfully replicated in a murine macrophage cell line in vitro and in primary immune cells in vivo suggested that immune cells may also support replication of human norovirus ( 7 ). (cdc.gov)

Below, we outline a method that is based on published protocols as well as our own experience by which one can differentiate hPSCs along the pancreatic lineage to generate insulin-producing beta-cell-like cells. (ca.gov)
Such cells from different sources can proliferate and differentiate into different lineages (e.g. osteogenic, chondrogenic and adipogenic) after suitable stimulation. (scirp.org)
Mesenchymal stem cells (MSCs) also known as mesenchymal stromal cells or medicinal signaling cells are multipotent stromal cells that can differentiate into a variety of cell types, including osteoblasts (bone cells), chondrocytes (cartilage cells), myocytes (muscle cells) and adipocytes (fat cells which give rise to marrow adipose tissue). (wikipedia.org)
While the terms mesenchymal stem cell (MSC) and marrow stromal cell have been used interchangeably for many years, neither term is sufficiently descriptive: Mesenchyme is embryonic connective tissue that is derived from the mesoderm and that differentiates into hematopoietic and connective tissue, whereas MSCs do not differentiate into hematopoietic cells. (wikipedia.org)
MSCs have been seen to even differentiate into neuron-like cells, but doubt remains about whether the MSC-derived neurons are functional. (wikipedia.org)
Researchers have discovered what enables embryonic stem cells to differentiate into diverse cell types and thus to be pluripotent. (sciencedaily.com)
Researchers of the Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch have discovered what enables embryonic stem cells to differentiate into diverse cell types and thus to be pluripotent. (sciencedaily.com)
C3H/10T1/2 cells can be induced to differentiate into multiple stem cell lineages. (naturalnews.com)
Differentiate human pluripotent stem cells (hPSCs) to cells of the endoderm lineage with our specialized cell culture media. (stemcell.com)
To directly differentiate to pancreatic cells, use our pancreatic progenitor kit to generate functional PDX1 + /NKX6.1 + cells that can be matured into insulin-producing beta cells. (stemcell.com)
It thoroughly investigated the unique ability of mouse embryonic stem cells to develop into cells of any organ ("pluripotency"), created new tools for functional genomic studies and thus provided key knowledge to understanding the commitment of cells to differentiate into cells of a particular germ layer. (europa.eu)
Dental pulp stem cells have been isolated from deciduous and permanent teeth and have the potential to self-renew and differentiate. (bvsalud.org)

Oct. 10, 2023 Generating specific cell lineages from induced pluripotent stem cells and embryonic stem cells is the holy grail of regenerative medicine. (sciencedaily.com)
Furthermore, the decreased expression of the EZH2 gene is crucial for stem cell differentiation into specific cell lineages involved in myogenesis, adipogenesis, osteogenesis, neurogenesis, and haematopoiesis [ 9 ]. (hindawi.com)

The origins and basis for these fat deposits are largely unknown, but there is a possibility that the altered mechanical and biological environments lead to dysregulation of MSC/MPC and progression to preferential differentiation towards the adipocyte lineage. (scirp.org)
1994). Furthermore, LOX participates in the dedication of pluripotent stem cells towards the adipocyte lineage (Huang et al. (exposed-skin-care.net)

Sandström J., Eggermann E., Charvet I., Roux A., Toni N ., Greggio C., Broyer A., Monnet-Tschudi F., Stoppini L. "Development and characterization of a human embryonic stem cell-derived 3D neural tissue model for neurotoxicity testing. (chuv.ch)
Gebara E., Bonaguidi M., Beckervordersandforth R., Sultan S., Udry F., Gijs P., Lie C., Ming G., Song H., Toni N . "Morpho-functional characterization of adult neural hippocampal stem cells reveals two morphotypes of radial glia-like cells. (chuv.ch)
Braun S., Moss J., Toni N. , Jessberger S. "Programming hippocampal neural stem/progenitor cells into oligodendrocytes enhances remyelination in the adult brain after injury" Cell Reports , 2015 Jun 23;11(11):1679-85. (chuv.ch)
Lorberbaum DS, Gottlieb D. ( 2011 ) Regulated expression of transgenes in embryonic stem cell-derived neural cells. (neurotree.org)
2008 ) Embryonic stem cells as a platform for analyzing neural gene transcription. (neurotree.org)
2006 ) Optimization of fibrin scaffolds for differentiation of murine embryonic stem cells into neural lineage cells. (neurotree.org)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)

However, details regarding the regulation of MSC/MPC proliferation and differentiation status are still unclear and it is likely that regulation involves both biological and mechanical influences in the different environments. (scirp.org)
It was also demonstrated that USF1 directly binds to the promoter region of 2,492 genes, which may be involved in the viral progression and cell proliferation pathways. (spandidos-publications.com)
Current Research and Scholarly Interests Regulation of self-renewal, proliferation and differentiation in adult stem cell lineages. (stanford.edu)
Developmental tumor suppressor mechanisms and regulation of the switch from proliferation to differentiation. (stanford.edu)
Correct regulation of self-renewal, proliferation, and differentiation into the proper downstream cell fate(s) is critical for tissue maintenance and repair, with defects contributing to tissue dismorphogenesis, aging, and cancer. (genetics-gsa.org)
I will be discussing our work on the regulation of proliferation and differentiation in the Drosophila male germ line adult stem cell lineage and the surprising contributions of cell type specific alternative mRNA processing to cell state changes. (genetics-gsa.org)
Malignant transformation and uncontrolled proliferation of an abnormally differentiated, long-lived hematopoietic progenitor cell results in a high circulating number of blasts, replacement of normal marrow by malignant cells, and the potential for leukemic infiltration of the central nervous system (CNS) and testes. (msdmanuals.com)
Acute Myeloid Leukemia (AML) In acute myeloid leukemia (AML), malignant transformation and uncontrolled proliferation of an abnormally differentiated, long-lived myeloid progenitor cell results in high circulating numbers. (msdmanuals.com)
Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)

Epigenetic Analysis of Human Embryonic Stem Cells: Basics, Computational Methods, Applications. (mpg.de)
Multivariate statistical analysis of epigenetic regulation with application to the analysis of human embryonic stem cells. (mpg.de)

The FUNGENES project aimed to achieve a detailed basic understanding of stem cell self-renewal and differentiation. (europa.eu)
At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)

This gradient approach may facilitate the screening of an optimal biomolecule surface density on tissue-engineered scaffolds, implants, or tissue culture ware to obtain the desired cell response, and may generate opportunities for more cost-effective regenerative medicine. (monash.edu)
Mesenchymal stem cells (MSCs) are the most commonly used cells in tissue engineering and regenerative medicine. (frontiersin.org)
The Stem Cells Portal provides coverage of the latest research, clinical applications, policies, and developments in the stem cell and regenerative medicine fields. (stemcellsportal.com)
The exceptional functional roles played by platelets and p-EVs explain the recent interest in exploring the use of allogeneic PCs as source material to develop new biotherapies that could address needs in cell therapy, regenerative medicine, and targeted drug delivery. (bvsalud.org)

Jarid2 is a PRC2 component in embryonic stem cells required for multi-lineage differentiation and recruitment of PRC1 and RNA Polymerase II to developmental regulators. (ox.ac.uk)
Collectively, these data indicate that transcriptional priming of bivalent genes in pluripotent ES cells is Jarid2-dependent, and suggests that priming is critical for subsequent multi-lineage differentiation. (ox.ac.uk)
During the last several years, his lab has developed novel methods for multi-lineage differentiation of pluripotent stem cells for cell therapy in mice models of muscular dystrophies and injuries. (uh.edu)

However, MSCs are found in much higher concentration in the Wharton's jelly compared to cord blood, which is a rich source of hematopoietic stem cells. (wikipedia.org)
We examined hematopoietic stem cells at four stages of differentiation, lineage-committed progenitors, and mature myeloid and lymphoid cells. (nih.gov)
Among bone marrow hematopoietic stem cells, 70% exhibited detectable telomerase activity. (nih.gov)
Hematopoietic stem cells (HSC) give rise to all types of blood lineages, including red blood cells (RBC). (lu.se)

Stem Cell Research & Therapy 14.1 (2023): 63. (bioinfor.com)

Aberration occurs in a stem cell that can give rise to multiple lineages. (medscape.com)

Feb. 21, 2022 Embryonic stem cells and other pluripotent cells divide rapidly and have the capacity to become nearly any cell type in the body. (sciencedaily.com)

Interestingly, the hMSCs differentiated on osteoblast ECM with adipogenic exosomes showed expression of adipogenic lineage genes, while hMSCs differentiated on adipocyte ECM with osteoblast exosomes showed osteogenic lineage genes. (exosome-rna.com)
Here we show that Jarid2 is a novel subunit of PRC2 that is required for the co-recruitment of PRC1 and RNAP to genes that regulate development in ES cells. (ox.ac.uk)
Jarid2-deficient ES cells showed reduced H3K4me2/me3 and H3K27me3 marking and PRC1/PRC2 recruitment, and did not efficiently establish Ser 5-phosporylated RNAP at target genes. (ox.ac.uk)
ES cells lacking Jarid2, in contrast to previously characterized PRC1 and PRC2 mutants, did not inappropriately express PRC2 target genes. (ox.ac.uk)
Promoter region motif analysis indicated that ETV5 has more binding motifs and genes than USF1 itself for USF1‑regulated DEGs, indicating that USF1 may indirectly modulate gene expression by regulating ETV5 expression in Huh7 cells. (spandidos-publications.com)
These include generation of knock-in reporter stem cell lines to study myogenic differentiation, CRISPR/Cas9 gene targeting methods to correct mutated genes in muscular dystrophies, and using bioscaffolds, seeded with stem cells, to treat skeletal muscle injuries. (uh.edu)
In particular, FUNGENES identified genes controlling the development of pluripotent embryonic stem (ES) cells into heart cells (cardiomyocytes), nerve cells (neurons), smooth muscle cells, vascular endothelial cells, fat cells (adipocytes), liver cells (hepatocytes) and insulin-producing cells of the pancreas. (europa.eu)

We investigated the role of crotonylation in hESC differentiation by introduce crotonate into the culture medium of GFP tagged LTR7 primed H9 cell and extended pluripotent stem cell lines. (bioinfor.com)

Stromal cells are connective tissue cells that form the supportive structure in which the functional cells of the tissue reside. (wikipedia.org)

This report addresses the issue of optimizing extracellular matrix protein density required to support osteogenic lineage differentiation of mesenchymal stem cells (MSCs) by culturing MSCs on surface-bound density gradients of immobilized collagen type I (COL1) and osteopontin (OPN). (monash.edu)

Wu J, Matthias N, Lo J, Ortiz-Vitali JL, Shieh AW, Wang SH, Darabi R . A Myogenic Double-Reporter Human Pluripotent Stem Cell Line Allows Prospective Isolation of Skeletal Muscle Progenitors. (uh.edu)
Assessment of the myogenic stem cell compartment following transplantation of pax3/pax7-induced embryonic stem cell-derived progenitors. (uh.edu)

Ando, W., Heard, B., Nakamura, N., Frank, C.B. and Hart, D.A. (2012) Ovine Synovial Membrane-Derived Mescenchymal Progenitor Cells Retain the Phenotype of the Original Tissue That Was Exposed to in Vivo Inflammation: Evidence for a Suppressed Chondrogenic Differentiation Potential of the Cells. (scirp.org)
Furthermore, MSCs can undergo osteogenic, adipogenic and chondrogenic differentiation ex vivo. (wikipedia.org)
After differentiation, BMP4 and FGF2 treated cells generally produce higher amounts of osteogenic and chondrogenic differentiation than untreated stem cells. (wikidoc.org)

Many research groups are engaged in using human pluripotent stem cells (hPSCs) to generate surrogate pancreatic beta-cells for transplantation into diabetic patients. (ca.gov)
Following his Ph.D., he performed a research fellowship in the lab of Jim Wells at Cincinnati Children's Hospital , where he turned his focus to understanding mechanisms that regulate embryonic development of endoderm-derived tissue (pancreas, liver, intestine) and utilizing human pluripotent stem cells (hPSCs) to understand human differentiation and development. (aiche.org)
However, traditional differentiation protocols of hPSCs to functional astrocytes, based on external cues to mimic development, are complex and time-consuming. (lu.se)

Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
Hart, D. (2014) Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
Kinetic and differentiation analyses indicate that both osteoblast and adipocyte exosomes augment ECM-mediated differentiation of hMSCs into the respective lineage. (exosome-rna.com)
The combination of osteoblast/adipocyte ECM and exosomes turned-on the lineage specific gene expressions at earlier time points of differentiation compared to the respective ECM or exosomes administered individually. (exosome-rna.com)
2009), which is inhibited during adipocyte differentiation (Dimaculangan et al. (exposed-skin-care.net)
In obese rats, the inhibition of LOX activity through -aminopropionitrile (BAPN, a particular inhibitor of LOX activity) decreases adipose cells fibrosis, partly corrects the adipocyte-size distribution design (moving it toward smaller sized sizes) and attenuates the upsurge in bodyweight and extra fat mass. (exposed-skin-care.net)

Batsali, A.K., Kastrinaki, M.C., Papadaki, H.A. and Pontikoglou, C. (2013) Mesenchymal Stem Cells Derived from Wharton's Jelly of the Umbilical Cord: Biological Properties and Emerging Clinical Applications. (scirp.org)
The term encompasses multipotent cells derived from other non-marrow tissues, such as placenta, umbilical cord blood, adipose tissue, adult muscle, corneal stroma, or the dental pulp of deciduous (baby) teeth. (wikipedia.org)

For pediatric patients with refractory cytopenia, certain cytogenetic abnormalities, or malignant transformation, hematopoietic stem cell transplantation (HSCT) from a matched related or unrelated donor early in the course of the disease is the treatment of choice. (medscape.com)
MDS is rare in childhood and may have a rapidly progressive course with an extremely poor prognosis without hematopoietic stem cell transplantation (HSCT). (medscape.com)
Treatment typically includes combination chemotherapy to achieve remission, intrathecal and systemic chemotherapy and/or corticosteroids for CNS prophylaxis, and sometimes cerebral irradiation for intracerebral leukemic infiltration, consolidation chemotherapy with or without stem cell transplantation, and maintenance chemotherapy for up to 3 years to avoid relapse. (msdmanuals.com)
Treatment selection takes into account patient age, fitness, and whether autologous stem cell transplantation (ASCT) is planned. (medscape.com)
This involves transplantation of developing midbrain cells from aborted fetuses, (the part that form mesDA neurons), into the striatum of a PD patient. (lu.se)
We still do not know what specific factors contribute to the success in transplantation i.e. what cells are responsible for motor recovery? (lu.se)
The aim of this thesis was to understand how particular factors such as neuronal content, placement and cell source, affect functional outcome after transplantation into the rodent brain. (lu.se)

Researchers from the West Virginia University School of Medicine demonstrate that osteoblasts-derived (Os-Exo) and adipocytes-derived (Ad-Exo) exosomes contain instructive factors that regulate the lineage specification of human mesenchymal stem cells (hMSCs). (exosome-rna.com)

Dr. Daniel Besser, Prof. Walter Birchmeier and Torben Redmer from the MDC, a member of the Helmholtz Association, used mouse embryonic fibroblasts (MEFs) in their stem cell experiments. (sciencedaily.com)
2012 ) Transgenic enrichment of mouse embryonic stem cell-derived progenitor motor neurons. (neurotree.org)
Combinatorial binding in human and mouse embryonic stem cells identifies conserved enhancers active in early embryonic development. (mpg.de)

We found that induced crotonylation in hESCs resulted in hESCs of different pluripotency states differentiating into the endodermal lineage. (bioinfor.com)
This pluripotency depends on a molecule -- E-cadherin -- hitherto primarily known for its role in mediating cell-cell adhesion. (sciencedaily.com)
If E-cadherin is absent, the stem cells lose their pluripotency. (sciencedaily.com)
In a first step they showed that the pluripotency of these stem cells is directly associated with the cell-adhesion molecule E-cadherin. (sciencedaily.com)
Thus, we have double proof that E-cadherin is directly associated with stem-cell pluripotency. (sciencedaily.com)
FUNGENES identified the gene subsets that are active in self-renewing cells, during the exit from pluripotency and at specific stages of mouse ES cell differentiation. (europa.eu)
cell authentication (STR), pluripotency and undifferentiated state confirmation, molecular karyotype, g-banding and iPS growth pattern and morphology analysis. (lu.se)

Mesenchymal stem/progenitor cells (MSC/MPC) are found in many tissues and fluids including bone marrow, adipose tissues, muscle, synovial membranes, synovial fluid, and blood. (scirp.org)
Li, L. and Jiang, J. (2011) Regulatory Factors of Mesenchymal Stem Cell Migration into Injured Tissues and Their Signal Transduction Mechanisms. (scirp.org)
Developing top down proteomics to maximize proteome and sequence coverage from cells and tissues. (chicagobiomedicalconsortium.org)
Adult stem cell lineages underlie most of the tissues in our body. (genetics-gsa.org)
Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)
The first steps toward a new cell culture system for human norovirus included the detection of human norovirus antigen in duodenal and jejunal enterocytes in tissue sections from human norovirus-infected transplant patients ( 11 ) and the development of human intestinal enteroids (HIEs) derived from nontransformed small intestine and colonic tissues ( 12 ). (cdc.gov)

[10] FGF2, in conjunction with BMP4 , promote differentiation of stem cells to mesodermal lineages. (wikidoc.org)

Through morphological changes, qPCR of pluripotent and germ layer-specific gene markers and flow cytometry analysis, we determined that the induced crotonylation resulted in hESC differentiating into the endodermal lineage. (bioinfor.com)
Computational analysis of genome-wide DNA methylation during the differentiation of human embryonic stem cells along the endodermal lineage. (mpg.de)

ECM of differentiated cells modulates phenotypic expression of stem cells, while their exosomes contain phenotype specific instructive factors (miRNA, RNA and proteins) that control stem cell differentiation. (exosome-rna.com)
As a newly discovered acylation modification, crotonylation of non-histone proteins remains largely unexplored, particularly in human embryonic stem cells (hESCs). (bioinfor.com)
It has been demonstrated to induce gremlin expression which in turn is known to inhibit the induction of differentiation by bone morphogenetic proteins . (wikidoc.org)
It is now well-established that mutations in TJ proteins are associated with a range of human diseases and that a change in the membrane fluidity of neighbouring cells can open possibilities for therapeutics to cross intercellular junctions. (bvsalud.org)
It has been suggested that this process relies on Notch's ability to inhibit E proteins, which are crucial for early B-cell development. (lu.se)

Sanchez-Pascual I., O'Laoi R., El M'Ghari I., Kocher-Braissant J., Ellisman MH, Toni N . "Fine processes of Nestin-GFP positive radial glia-like stem cells in the adult dentate gyrus ensheathe local synapses and vasculature. (chuv.ch)
Some adult stem cell niches have already been described, but the majority of them remain unclear, including the dental pulp stem cell niches. (bvsalud.org)

Understanding the molecular relationships is essential for using human somatic cells to develop stem cell therapy for diseases such as heart attack, Alzheimer's or Parkinson's disease or diabetes," Dr. Besser said. (sciencedaily.com)
[3] Generally, the 155 aa/18 kDa low molecular weight (LMW) form is considered cytoplasmic and can be secreted from the cell, whereas the high molecular weight (HMW) forms are directed to the cell's nucleus. (wikidoc.org)
Comparative molecular portraits of human unfertilized oocytes and primordial germ cells at 10 weeks of gestation. (mpg.de)
They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. (sigmaaldrich.com)
Consequently, investigation of molecular heterogeneity in the differentiation potential of HSPC is vital to identify novel regulators that affect generation of specific cell types, especially RBC. (lu.se)
basic characterization, including cell authentication (STR), undifferentiated state (FACS) and molecular karyotyping. (lu.se)
Little is known about molecular mecha- desmosomal glycoproteins, resulting in the nisms affecting mast cell and T lympho- loss of keratinocyte cell-cell adhesion [ 1 ]. (who.int)

Loss of individual PRC components in ES cells can lead to gene de-repression and to unscheduled differentiation. (ox.ac.uk)
2007 ) Polony analysis of gene expression in ES cells and blastocysts. (neurotree.org)
A data integration approach to mapping OCT4 gene regulatory networks operative in embryonic stem cells and embryonal carcinoma cells. (mpg.de)
Its major objective was to produce a gene expression atlas covering the development of ES cells into all three germ layers (ecto-, meso-, and endoderm) and into somatic cells. (europa.eu)

Studies of fundamental astrocyte biology in humans and their role in neurological disease have been hindered by shortage of native human astrocytes for research purposes and inadequate animal and cell models. (lu.se)
However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)

Cell Stem Cell 2018 Apr 18. (chuv.ch)

MDS is a clonal disorder of myeloid stem cells. (medscape.com)
C3H/10T1/2 is a clonal mouse embryo line used in the developmental determination of vertebrate stem cell lineages and their subsequent differentiation. (naturalnews.com)
Myelodysplastic syndrome (MDS) in childhood encompasses a diverse group of bone marrow disorders that share a common clonal defect of stem cells and that result in ineffective hematopoiesis with dysplastic changes in the marrow. (medscape.com)

Gimble, J.M., Bunnell, B.A. and Guilak, F. (2012) Human Adipose-Derived Cells: An Update on the Transition to Clinical Translation. (scirp.org)

Epigenetic regulation and transcriptional reprogramming both play critical roles during stem cell differentiation and lineage commitment. (stemcellsportal.com)
The therapeutic application of stem cells requires the elucidation of how epigenetic factors control lineage commitment. (stemcellsportal.com)
The Notch signaling pathway is involved in several lineage commitment and differentiation events. (lu.se)
These data suggest that Notch signaling may affect B- versus T-lineage commitment by the targeting of both EBF and E2A. (lu.se)

May 20, 2020 In the bone marrow, blood stem cells via precursor cells give rise to a variety of blood cell types with various functions: white blood cells, red blood cells, or blood platelets. (sciencedaily.com)
2020 ) Chimeric antigen receptor T-cell therapy after allogeneic stem cell transplant for relapsed/refractory large B-cell lymphoma. (neurotree.org)

No immunoreactivity is seen with murine EC, EG or ES cells. (sigmaaldrich.com)

Genetic abnormalities associated with MDS block differentiation of hematopoietic stem and progenitor cells. (medscape.com)
The paths open vs. closed to evolutionary exploitation are dictated by the population-genetic environment, which varies substantially among phylogenetic lineages. (genetics-gsa.org)
Understanding the genetic pathways underlying differentiation of ES cells to somatic cells would contribute to future therapeutic strategies for degenerative diseases such as heart disease, diabetes and Parkinson's. (europa.eu)
The 2016 World Health Organization (WHO) classification of lymphoid neoplasms incorporates genetic data, clinical features, cell morphology, and immunophenotype, all of which have important implications for disease prognosis and management. (msdmanuals.com)

Based on these results, the researchers concluded that epimedin C effectively promotes vascularization both in the BMP2-dependent bone formation model and in the 4T1 mammary tumor-bearing model by inducing the endothelial-like differentiation of C3H/10T1/2 in vivo. (naturalnews.com)
The HIEs, or "mini-guts," are generated from stem cells present in the intestinal crypts isolated from human intestinal tissue and cultured indefinitely as ex vivo, 3-dimensional (3D) cultures in growth-factor-enriched media ( 13 - 15 ). (cdc.gov)

While both processes result in new blood vessel formation, vasculogenesis occurs mainly during embryonic development and is facilitated by EPCs, while angiogenesis takes place via "sprouting" of endothelial cells , which allows new blood vessels to take shape from already existing blood vessels. (naturalnews.com)
Finally, the researchers reported that the mRNA levels of endothelial markers - namely, the platelet endothelial adhesive factor-1 (CD31), the endothelial cell-specific molecule-1 (ESM-1), and the vascular von Willebrand factor (vWF) in mouse 4T1 mammary tumor tissue - significantly increased after epimedin C treatment. (naturalnews.com)
Fibroblast growth factor protein was first purified in 1975, but soon afterwards others using different conditions isolated basic FGF, Heparin-binding growth factor-2, and Endothelial cell growth factor-2. (wikidoc.org)
6- Differentiation of iPSCs into cardiomyocytes and endothelial cells for disease modeling and screening. (uh.edu)
Paracellular permeability across epithelial and endothelial cells is, in large part, regulated by apical intercellular junctions also referred to as tight junctions (TJs). (bvsalud.org)

fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)

Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
Shane Grealish: Cell Replacement Therapy for Parkinson's Disease: The Importance of Neuronal Subtype, Cell Source and Connectivity for Functional Recovery. (lu.se)
Through understanding functional recovery in terms of neuronal subtype and connectivity, the work presented in this thesis aims to bring the prospect of CRT closer to the clinic, I also describe the generation of a very promising alternative cell source that could rival fetal tissue. (lu.se)

Current Research and Scholarly Interests The thread of Ariadne that connects germ cells, preimplatation development and pluripotent stem cells is the focus of my research, with a specific interest in human development. (stanford.edu)
Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). (sigmaaldrich.com)
This antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). (sigmaaldrich.com)
Germ cells are the only cells in our body destined to generate a completely new organism. (genetics-gsa.org)
I will be discussing how primordial germ cells are set aside from other cells in the body to fulfill their unique function in reproduction and how we have started to probe the quality control mechanisms that regulate the faithful transmission of cytoplasmic structures including mitochondria from the egg to the embryo. (genetics-gsa.org)

Our study reveals especially the transition between hepatic specification and hepatic maturation as dependent on mitochondrial respiration and demonstrates that even though differentiating cells are primarily dependent on glycolysis until induction of hepatocyte maturation, oxidative phosphorylation is essential at all stages of differentiation. (ku.dk)

Lineage specification is an essential process in stem cell fate, tissue homeostasis and development. (exosome-rna.com)
The MDC researchers found that in contrast to the original cells, the new pluripotent cells derived from mouse connective tissue contained E-cadherin. (sciencedaily.com)
MSCs can promote host tissue repair through several different mechanisms including donor cell engraftment, release of cell signaling factors, and the transfer of healthy organelles to the host. (frontiersin.org)
Like other FGF family members, basic fibroblast growth factor possess broad mitogenic and cell survival activities, and is involved in a variety of biological processes, including embryonic development , cell growth , morphogenesis , tissue repair , tumor growth and invasion. (wikidoc.org)
To date, a lot of EZH2 variants have been found in various cell and tissue types [ 18 - 20 ]. (hindawi.com)
Using model organisms to understand mechanisms by which the embryo develops, with a focus on specification, differentiation and organogenesis of tissue derived from the endoderm. (aiche.org)
Niches are special microenvironments in tissue where stem cells are located. (bvsalud.org)
Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)
Use of human fetal tissue raises several ethical issues, but are there alternative cell sources that can substitute effectively? (lu.se)

One of these is fate determination of the common lymphoid progenitor, promoting T-cell development at the expense of B-cell differentiation. (lu.se)

The telomerase-expressing somatic cells observed in this study are not thought to be immortal, and expression was not correlated with cell cycle distribution or differentiation state. (nih.gov)
The molecule also plays a crucial role in the reprogramming of somatic cells (body cells) into pluripotent stem cells. (sciencedaily.com)
In a second step the researchers investigated what happens when somatic cells that normally neither have E-cadherin nor are pluripotent are reprogrammed into a pluripotent stem cell state. (sciencedaily.com)
In this reprogramming technique, somatic cells are converted into induced pluripotent stem cells (iPSCs). (sciencedaily.com)
E-Cadherin is necessary for maintaining pluripotent stem cells and also for inducing the pluripotent state in the reprogramming of somatic cells," Dr. Besser said. (sciencedaily.com)
If E-cadherin is absent, somatic cells cannot be reprogrammed into viable pluripotent cells. (sciencedaily.com)

Hart, D.A. (2014) Why Mesenchymal Stem Cell/Progenitor Cell Heterogeneity in Specific Environments? (scirp.org)

However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
A potentially pre-clinical aspect of this thesis is detailed in paper №4 where I describe a robust protocol for the generation of functional mesDA neurons from human embryonic stem cells that are functional in a rat model of PD. (lu.se)

Mitochondrial function or the ability to generate energy through OXPHOS (oxidative phosphorylation) is vital for cell homeostasis and its dysfunction has been linked to the pathogenesis of nearly all chronic diseases ( Pieczenik and Neustadt, 2007 ). (frontiersin.org)
During this time, he pioneered efforts to generate 3-dimensional intestinal organoids from human pluripotent stem cells using an approach know as directed differentiation. (aiche.org)
that is, they have the ability to generate other stem cells and perpetuate themselves. (bvsalud.org)

Australia allowed the other download Human Embryonic and Induced Pluripotent Stem Cells: Lineage Specific Differentiation Protocols to impose software for methods in 1902 and Canada was also system centuries as emotional Goodreads by video until 1922. (idealnaja.pl)
We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
Finally, we provide protocols for analyzing these cells through flow cytometry and immunocytochemistry. (lu.se)

Next, the MDC researchers want to find out to what extent E-cadherin also regulates human embryonic stem cells. (sciencedaily.com)

Microenvironmental milieu consists of two essential components, one being extracellular matrix (ECM) as the substratum, while the other being cell secreted exosomes and growth factors. (exosome-rna.com)
The cells, which are long and thin, are widely dispersed, and the adjacent extracellular matrix is populated by a few reticular fibrils, but is devoid of the other types of collagen fibrils. (wikipedia.org)
Could extracellular vesicles derived from mesenchymal stem cells be a potential therapy for acute pancreatitis-induced cardiac injury? (wjgnet.com)
Bennett J, Baumgarten SC, Stocco C. GATA4 and GATA6 Silencing in Ovarian Granulosa Cells Affects Levels of mRNAs Involved in Steroidogenesis, Extracellular Structure Organization, IGF-I Activity, and Apoptosis. (chicagobiomedicalconsortium.org)
Lysyl oxidase (LOX) activity, which governs extracellular matrix maturation, is vital for cells fibrosis. (exposed-skin-care.net)
In addition, platelets are prone to release in the blood circulation a unique set of extracellular vesicles (p-EVs), which carry a rich biomolecular cargo influential in cell-cell communications. (bvsalud.org)

Microenvironmental cues provide direct and selective extrinsic signals to regulate lineage specification of stem cells. (exosome-rna.com)

The proper methylation status of histones is essential for appropriate cell lineage and organogenesis. (hindawi.com)

Borlongan, C.V., Glover, L.E., Tajiri, N., Kaneko, Y. and Freeman, T.B. (2011) The Great Migration of Bone Marrow-Derived Stem Cells towards the Ischemic Brain: Therapeutic Implications for Stroke and Other Neurological Disorders. (scirp.org)
These bone marrow stem cells do not contribute to the formation of blood cells, and so do not express the hematopoietic stem cell marker CD34. (wikipedia.org)
They are sometimes referred to as bone marrow stromal stem cells. (wikipedia.org)
These cells have several advantages over bone-marrow-derived MSCs. (wikipedia.org)
This event explains the presence of multiple derangements observed in the bone marrow that involve several cell lineages. (medscape.com)
As the affected cell lines continue to divide and to provide the marrow with dysplastic cells, bone marrow dysfunction becomes apparent. (medscape.com)
Since the researchers already knew that vasculogenesis plays a pivotal role in bone formation, they used a bone morphogenetic protein 2 (BMP2)-induced ectopic bone formation model and mice 4T1 breast cancer cells co-implanted with luciferase-labeled C3H/10T1/2 cells (4T1 + C3H/10T1/2-Luc) model for their study. (naturalnews.com)

3. developed new embryonic stem cell-based approaches to screening of small candidate molecules for therapeutic applications in human diseases. (europa.eu)

Anatomy66

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