Line u937 cells. Medical search. Frequent questions

U-937 cells mature and differentiate in response to a number of soluble stimuli, adopting the morphology and characteristics of monocytes, macrophages or dendritic cells. (wikipedia.org)
We investigated the effects of monocyte-activating stimuli (IFN-gamma, IL-2, LPS, TNF-alpha, and GM-CSF) on the expression and function of CD38, starting from the observation that human monocytes and the derived lines U937, THP-1, and Mono-Mac-6 bear the molecule on their surface. (nih.gov)
Treatment with IFN-gamma produced a dose- and time-dependent up-regulation of CD38 in monocytes and monocytic lines, which was paralleled by increased ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase activities. (nih.gov)
To do this, we used a previously described clonal line of IRF3/IRF7 double knockout human THP1 monocytes (13). (woofahs.com)
B) PMA-differentiated human U937 monocytes or two clonal lines of STING KO U937 cells were treated with the indicated ligands for 16 hours before harvest and RT-qPCR analysis of mRNA expression. (woofahs.com)
Monocytes (MC) are bone marrow (BM) derived mononuclear phagocytes that play an important role in innate immune response and are the major immune cell population in chronic tissue inflammatory ( 1 , 2 ). (frontiersin.org)
The interleukin-1 (IL-1) responses of U937 cells and peripheral blood monocytes to organic dusts were compared. (cdc.gov)
Extracts of bulk cotton samples were tested with U937 cells and monocytes. (cdc.gov)
However, we know less about the biological effects of IL-21 and its signaling mechanisms in leukemia cells and monocytes. (intechopen.com)
However, the features initially ascribed to endothelial progenitor cells, i.e ., phenotype, expression of endothelial markers, and ability to form tube-like structures on Matrigel, can be mimicked by blood monocytes [ 3 ]. (hindawi.com)
The two monoclonal antibodies MAX.1 and MAX.11 recognize cell surface antigens that are almost undetectable on monocytes but highly expressed on differentiated macrophages. (uni-regensburg.de)

Due to limited possibilities for obtaining primary macrophages, U-937 cell line, beside the THP-1 cells, is a crucial tool for studying these phagocytes in vitro. (wikipedia.org)
Whereas, in the case of THP-1 cells, the polarization is reversed in favor of classical M1 macrophages. (wikipedia.org)
Electrochemical potential zone of viability on CoCrMo surfaces is affected by cell type: Macrophages under cathodic bias are more resistant to killing. (musc.edu)
Materials and Methods: We established four different macrophage subtypes through introduction of different culture conditions to THP-1- and U937-derived macrophages. (iiarjournals.org)
Changes in cellular enzymes and extracellular release of lysosomal acid hydrolases in macrophages exposed to a group A streptococcal cell wall substance. (microbiologyresearch.org)
A and B ) HIV-1 NLYU2 replication in CD4+ T cells ( A ) or macrophages ( B ) treated with AhR agonist or antagonist. (elifesciences.org)
A-H ) HIV-1 replication in CD4+ T cells ( A ) PBMCs ( B-D ) or macrophages ( E-H ), using strain HIV-1 NLYU2 or HIV-1 89.6 , as indicated. (elifesciences.org)
I ) Comparison of HIV-1 supernatant reverse transcriptase levels, at four days after infection in infected macrophages, CD4+ T cells or PBMCs treated with AhR agonist or antagonist. (elifesciences.org)
Effect of AhR activation on STAT1, cell-cycle and gene expression in macrophages. (elifesciences.org)
The shaded histograms represents unstained macrophages and the dotted line represents isotype controls. (elifesciences.org)
In the present study, I demonstrated that a brief exposure to LeTx caused a continual MEK1 cleavage and prevented TNF-ce production in non-proliferating cells such as human peripheral blood mononuclear cells or mouse primary peritoneal macrophages. (uwo.ca)

A human cell line established from a diffuse histiocytic lymphoma (HISTIOCYTIC LYMPHOMA, DIFFUSE) and displaying many monocytic characteristics. (umassmed.edu)
ErbB2/Her2 was detected in both live and fixed SK‑BR‑3 human breast cancer cell line and but not detected in fixed U937 human histiocytic lymphoma cell line. (rndsystems.com)

This study describes the susceptibility to dengue virus infection of a monocytic cell line at different states of differentiation. (microbiologyresearch.org)
F ) HIV-1 NLYU2 replication in a PMA-differentiated monocytic cell line (U937 CD4/R5) transduced with a lentiviral vectors (CSIB) expressing the indicated genes that were upregulated in response to the AhR agonist (from panel ( E )). Representative example of 2 independent experiments. (elifesciences.org)

Cancer immunotherapy involves activating the immune system to recognize and attack tumor cells. (iiarjournals.org)

Human peripheral blood mononuclear cells were used in the evaluation of IL-1beta production. (cdc.gov)
and high glucose in mononuclear cells? (musc.edu)
Toll-like receptor 4 activation in microvascular endothelial cells triggers a robust inflammatory response and cross talk with mononuclear cells via interleukin-6. (musc.edu)
Different signaling mechanisms regulating IL-6 expression by LPS between gingival fibroblasts and mononuclear cells: seeking the common target. (musc.edu)
We used G-CSF-mobilized CD34 + HSPCs, FACS-sorted from healthy donor peripheral blood mononuclear cells (PBMCs). (hindawi.com)

The unique feature of these cells is the absence of cellular disintegration as an accompanying phenomenon of apoptosis. (wikipedia.org)
It was documented that the basic molecular processes (degradation of DNA, PS externalization, caspase activity or CytC release) following the induction of apoptosis are not drastically changed in these cells. (wikipedia.org)
Microscopic analysis, flow cytometry, and WST-1 tests were performed on the U937 cell line after 72 hr of treatment with different concentrations of ethanol pod extract from Acacia nilotica ( A. nilotica ) to test for DNA cell cycle, cell viability and apoptosis, and to determine the IC 50 of the drug. (phcogres.com)
A reduction in cell viability with an increase of cells in the late apoptosis stage by flow cytometry indicated the cytotoxic effect of A. nilotica and accumulation of cells in the sub-G1 phase of the DNA cell cycle analysis. (phcogres.com)
We previously found that carbamate pesticides induced significant apoptosis in human natural killer cells. (researchgate.net)
To investigate whether carbamate pesticides also induce apoptosis in human T lymphocytes, in the present study Jurkat human T cells were treated in vitro with thiram, maneb, carbaryl or ziram. (researchgate.net)
It produces a cytocidal distending toxin (HdCDT), which causes cell cycle arrest and apoptosis/necrosis of human cells and contributes to the aggravation of ulcers. (medscape.com)
Its roles include regulation of proliferation, promotion of immune system and activation of apoptosis in B cells. (intechopen.com)
In these cells, IL-21 does not promote proliferation but enhances apoptosis and chemotaxis. (intechopen.com)
Of note, an increased carbohydrate uptake and/or induction of death receptors of cancer cells was exploited to develop glycoconjugates that potentially induce cellular stress, ROS and apoptosis. (degruyter.com)
Ectopic Mcl-1 expression attenuates Bak activation and apoptosis by ABT-737 + roscovitine, whereas cells overexpressing Bcl-2 or Bcl-xL remain fully sensitive. (aacrjournals.org)
HA14-1) that disable Bcl-2, resulting in induction of apoptosis in leukemia cell lines ( 8 ). (aacrjournals.org)
Cell cycle analysis showed a significant increase in the accumulation of KSO-SFE-treated cells at sub-G1 phase, indicating the induction of apoptosis by KSO-SFE. (hindawi.com)
ADAR1 silencing in breast cancer cells leads to increased apoptosis, suggesting an anti-apoptotic function that promotes cancer progression. (cancerindex.org)

Confocal immunofluorescent analysis of Ramos cells, untreated (left) or IgM-treated (middle), and Jurkat cells (right) using Phospho-CD79A (Tyr182) Antibody (green). (cellsignal.com)

Due to the relative uniformity of expanded cultures and lower maintenance requirements these cells have been since used as an important tool for studying phagocyte differentiation and different kinds of cell-to-cell interactions. (wikipedia.org)
Further differentiation into dendritic cells can be induced by exposing them to self-peptide from apolipoprotein E (Ep1.B). This treatment leads to changes in surface marker expression and thus induces acquirement of DC-like morphology. (wikipedia.org)
Ly6C high MC presented downregulated co-stimulatory receptors (CD2, GITR, and TIM1) which direct immune cell proliferation, and upregulated co-stimulatory ligands (LIGHT and SEMA4A) which trigger antigen priming and differentiation. (frontiersin.org)
It induces differentiation of AML cell lines, primary AML cells, and granulocytic differentiation of HSCs. (tocris.com)
Vitamin D3-induced monocytic differentiation resulted in an increased carboxypeptidase M expression in all three cell lines. (uni-regensburg.de)
Semaphorin 4D (Sema4D) belongs to the semaphorin family, which binds to receptors such as Pleixn-B1 (transmembrane structure receptor), CD72 (cluster of differentiation antigen 72, mainly expresses on lymphocyte cells), then activates downstream signaling molecules. (jcancer.org)
It inhibits cytokine production and cell proliferation/differentiation in various immune cells. (uwo.ca)

In this study, they also confirm that the U-937 cells do respond to a model JAK3 inhibitor, and their proliferation is thus impaired by it. (wikipedia.org)
Most cancer cells undergo metabolic reprogramming that support their survival and proliferation. (degruyter.com)
To test whether a magnetic field of 10 µT and 100 µT influences the proliferation of a neuroblastoma cell line , alone or in combination with retinoic acid (a retinoid applied in oncostatic therapies). (emf-portal.org)
The experiments showed that exposure to magnetic fields of 10 µT or 100 µT significantly increased the proliferation and the DNA content when compared to the control samples, but did not influence the cell viability and the total protein content. (emf-portal.org)
However, the second experiment showed that a treatment with retinoic acid significantly reduced the proliferation in comparison to the control cell culture , independent if the cell cultures were exposed or sham exposed . (emf-portal.org)
The authors conclude that a magnetic field exposure of 10 µT or 100 µT could enhance the cell proliferation in a neuroblastoma cell line and that retinoic acid may inhibit this effect. (emf-portal.org)
This study demonstrated that modulation of PI3K/Akt/GSK3 signaling cascades can be beneficial for protecting or facilitating recovery from cellular LeTx intoxication in cells that depend on basal MEK1 activity for proliferation. (uwo.ca)
Here, we modulate SH3BGRL expression level in two liver cancer cells and conduct both in vitro and in vivo analyses of SH3BGRL in cell proliferation and tumorigenesis. (bvsalud.org)
Results demonstrate that SH3BGRL notably inhibits cell proliferation and arrests the cell cycle in both LO2 and HepG2 cells. (bvsalud.org)
The xenograft mouse model reveals that SH3BGRL overexpression can efficiently suppress tumorigenesis in vivo, while the additional silencing ATG5 in SH3BGRL-overexpressing cells attenuates the inhibitory effect of SH3BGRL on both hepatic tumor cell proliferation and tumorigenicity in vivo. (bvsalud.org)

Ly6C high MC exhibited activated neutrophil degranulation, lysosome, cytokine production/receptor interaction and myeloid cell activation pathways, and Ly6C low MC presented features of lymphocyte immunity pathways in both mice. (frontiersin.org)
In addition, carboxypeptidase M expression could be detected in HL-60, U937, and THP-1 myeloid cell lines. (uni-regensburg.de)

2022), the U-937 cell line specifically could be a promising model for acute myeloid leukemia, as it was shown to actively express the JAK3M511I activated mutation observed in about 10 % of AML patients. (wikipedia.org)
To explore and evaluate the cytotoxic effect of A. nilotica, we used Acacia nilotica's pod extract on U937 cell lines as an acute myeloid leukemia model. (phcogres.com)
Transcriptional Regulation of JARID1B/KDM5B Histone Demethylase by Ikaros, Histone Deacetylase 1 (HDAC1), and Casein Kinase 2 (CK2) in B-cell Acute Lymphoblastic Leukemia. (musc.edu)

In the present study, we demonstrate that following exposure to plasma from diabetic patients, the human promonocytic cell line U937 exhibits a significant elevation in core 2 GlcNAc-T activity and increased adherence to cultured retinal capillary endothelial cells. (diabetesjournals.org)
We previously found that ziram, a carbamate pesticide, significantly reduced perforin, granzyme A (GrA), granzyme B (GrB), granzyme 3/K (Gr3/K), and granulysin (GRN) levels in NK-92MI cells, a human natural killer (NK) cell line. (researchgate.net)
To investigate whether other carbamate pesticides also show similar toxicity on human NK cells, we conducted further exp. (researchgate.net)
The following day cells were either left untreated (solid line) or treated (dotted line) with recombinant human IFN-γ (Cat. (bdbiosciences.com)
These findings reveal the existence, sensor, a specific downstream target, and viral antagonists of a STING-independent DNA sensing pathway (SIDSP) in human cells. (woofahs.com)
Second, unlike mouse cells, human cells possess a robust STING-independent DNA sensing pathway that is delayed relative to the cGAS-STING pathway. (woofahs.com)
Third, IRF3 and IRF7 are essential for both DNA-activated antiviral responses in human cells. (woofahs.com)
Thus, human cells C but not mouse cells C have a robust STING-independent DNA sensing pathway (SIDSP). (woofahs.com)
Open in a separate window Figure 2: A STING-independent DNA sensing pathway (SIDSP) in human cells(A) Primary mouse embryonic fibroblasts were treated with Lipofectamine alone (Lipo) or the indicated ligands for four hours before harvest and quantitative RT-PCR (RT-qPCR) analysis of mRNA expression. (woofahs.com)
Human MC were initially divided into three subsets based on the cell surface expression of CD14 and CD16, and recently classified based on CD40 expression ( 2 - 5 ). (frontiersin.org)
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using Btk (D3H5) Rabbit mAb performed on the Leica ® Bond ™ Rx. (cellsignal.com)
Immunoprecipitation of Phospho-Btk (Tyr223) from Ramos cells, serum-starved followed by treatment with anti-human IgM (12 μg/ml, 10 min) using Phospho-Btk (Tyr223) (D1D2Z) Rabbit mAb. (cellsignal.com)
Interleukin-21 (IL-21) was first identified in 2000 through screening of a cDNA library from CD3+ human T cells [ 1 ]. (intechopen.com)
Here, we show that Mcl-1 down-regulation by the cyclin-dependent kinase (CDK) inhibitor roscovitine or Mcl-1-shRNA dramatically increases ABT-737 lethality in human leukemia cells. (aacrjournals.org)
Extraction from 9 parameters of KSO-SFE was screened for cytotoxicity towards human colorectal cancer cell lines (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines using MTS assay. (hindawi.com)
Previous study has shown that kenaf seed oil extracted by SFE (KSO-SFE) is cytotoxic towards human cervical cancer cell line [ 7 ]. (hindawi.com)
Human colorectal cancer (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines were purchased from the American Type Culture Collection (ATCC), USA. (hindawi.com)
Conditioned media from such cells were then used to stimulate human aortic endothelial cells (HAoECs) to enhance tube-like structure formation in a Matrigel assay. (hindawi.com)
Concluding, high concentrations of atorvastatin stabilize tubes and enhance the paracrine angiogenic activity of human PAC cells in vitro . (hindawi.com)
In summary, the present data could be of potential relevance to identify the mechanisms of action by which extremely low frequency magnetic fields affect human cells . (emf-portal.org)
Replication of dengue and Junin viruses in cultured rabbit and human endothelial cells. (microbiologyresearch.org)
Infection enhancement of dengue type 2 virus in the human U-937 monocyte line by antibodies to Flavivirus cross-reactive determinants. (microbiologyresearch.org)
Detection of ErbB2/Her2 in MCF-7 human cell line by Flow Cytometry. (rndsystems.com)
MCF-7 human cell line was stained with Biotinylated Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Monoclonal Antibody (Catalog # FAB9589B, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Streptavidin (Catalog # F0050 ). (rndsystems.com)
ErbB2/Her2 in SK-BR-3 Human Cell Line. (rndsystems.com)
SK-BR-3 live cells were incubated for 1 hour at 4°C with Anti-Human ErbB2/Her2 Biotinylated Monoclonal Antibody (FAB9589B) diluted at 5 µg/mL with HBSS. (rndsystems.com)
SK-BR-3 and U937 fixed cells were incubated for 2 hours at room temperature with Anti-Human ErbB2/Her2 Biotinylated Monoclonal Antibody (FAB9589B) diluted with antibody diluent. (rndsystems.com)
We benchmarked PG2 using synthetic data and genomic, transcriptomic, and proteomic analysis of human leukemia cells. (biorxiv.org)
In human monocytic cell lines U-937 and THP-1, LeTx induced cell cycle arrest in Go-Gi phase by rapid down-regulation of cyclin D1/D2 and checkpoint kinase 1 through MEK1 inhibition. (uwo.ca)

A large body of evidence now implicates increased leukocyte-endothelial cell adhesion as a key early event in the development of diabetic retinopathy. (diabetesjournals.org)
We recently reported that raised activity of the glycosylating enzyme core 2 β 1,6- N -acetylglucosaminyltransferase (GlcNAc-T) through protein kinase C (PKC)β2-dependent phosphorylation plays a fundamental role in increased leukocyte-endothelial cell adhesion and capillary occlusion in retinopathy. (diabetesjournals.org)
The exact pathogenic mechanism by which capillary nonperfusion, as demonstrated by fluorescein angiography, occurs is still unclear, but recent experimental animal studies ( 3 - 5 ) demonstrate that increased leukocyte entrapment in retinal capillaries of diabetic rats is an early event associated with areas of capillary nonperfusion and the development of endothelial cell damage. (diabetesjournals.org)
Neither AT alone in control media nor conditioned media nor AT-stimulated cells affected numbers of endothelial cells in the plug or plug's vascularization. (hindawi.com)
The process of neovascularization can be influenced not only by the residual endothelial cells but also by the circulating cells. (hindawi.com)
Such myeloid angiogenic cells (MAC) [ 1 ] of bone marrow origin were known as endothelial progenitor cells (EPCs). (hindawi.com)
Cells previously known as endothelial progenitor cells are considered now to be rather myeloid angiogenic cells than endothelial precursors [ 4 ]. (hindawi.com)

The cells were analyzed on a BD FACSCalibur™ flow cytometry system. (bdbiosciences.com)
Krutzik, P.O. & Nolan, G.P. Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling. (nature.com)

Illustrations of the cytotoxic activity of A. nilotica pod extract on U937 after 72 hr of A. nilotica pod extract treatments. (phcogres.com)
DT could be extremely cytotoxic for eukaryotic cells. (org.ua)

Myeloid angiogenic cells (MAC) derive from hematopoietic stem/progenitor cells (HSPCs) that are mobilized from the bone marrow. (hindawi.com)
In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)

The immunosensor was successfully applied in the detection and quantification of PB1-F2 in infected mouse lungs and cell lines, providing temporal expression profiles of PB1-F2 during viral infection. (omicsonline.org)
To introduce altered Drosophila Gap1 transgenes into flies, the sevenless ( sev ) enhancer/promoter cassette, an eye-specific promoter, was used to drive expression in cone cell precursors at appropriate levels. (sdbonline.org)
In addition, these two binding sites play different roles in the regulation of Sema4D expression in HUVEC and Caco-2 cells. (jcancer.org)
Our recombinant nontoxic toxin analogues and derivatives were applied in studying of toxin interaction with cell surface, intracellular transport of DT and in determination of proHB-EGF expression level. (org.ua)
Finally, quantitative PCR (qPCR) and western blot analysis showed that expression level of RB1 in apatinib-resistant cell as well as the samples of patients in progressive disease were significantly lower than that in controls. (bvsalud.org)
Molecularly, SH3BGRL upregulates the expression of ATG5 from proteasome degradation as well as the inhibitions of Src activation and its downstream ERK and AKT signaling pathways, which eventually enhance autophagic cell death. (bvsalud.org)

U-937 line can be cultured in vitro for up to 3 months or stored frozen in -80°C for several years following an appropriate protocol. (wikipedia.org)
Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo . (hindawi.com)

These cells also stain positive for α-napthhyl butyrate esterase, produce lysozymes and are phagocytic (both latex beads and sensitised erythrocytes). (sigmaaldrich.com)

This indicates the intricate control of metabolism and redox balance for survival of cancer cells that can be targeted by therapeutic agents. (degruyter.com)

U937 Cells" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)

In PAE cells, soluble Sema4D promoted cell migration and developed a "tubulogenic" phenotype ( 4 ). (jcancer.org)
Furthermore, studies have shown HIF-mediated Sema4D induction in the generation of the pro-angiogenic phenotype in oral squamous cell carcinoma (OSCC) and have determined its biological significance for tumor growth and vascularity ( 10 ). (jcancer.org)

It induces CDK2 degradation in several AML cell lines, including NB4, U937, OCI-AML2, OCI-AML3 and Kasumi-1 cells. (tocris.com)

The chemokine CCL2, which pared to GPT, the LLNA reduces animal numbers needed, can recruit dendritic cells into the site of inflammation, is improves animal welfare, and decreases experimental time also upregulated in keratinocytes following hapten exposure. (cdc.gov)

MS = A highly conserved 76-amino acid peptide universally found in eukaryotic cells that functions as a marker for intracellular PROTEIN TRANSPORT and degradation. (doctorinternet.com)

Moreover, pattern recognition receptors, such as Toll-like receptors 1 through 9 (TLR1 to TLR9), have specific subcellular localization to either cell or lysosomal membranes, as well as specific ligands, including microbial components, viral RNAs and CpG DNAs. (iiarjournals.org)

Mass cytometry: technique for real-time single cell multitarget immunoassay based on inductively coupled plasma time-of-flight mass spectrometry. (nature.com)
Majonis, D., Ornatsky, O., Kinach, R. & Winnik, M.A. Curious results with palladium- and platinum-carrying polymers in mass cytometry bioassays and an unexpected application as a dead cell stain. (nature.com)

U-937 cells (ATCC CRL-1593) were serum starved overnight (RPMI containing 0.1% FCS). (bdbiosciences.com)

U-937 cells are of myeloid lineage and thus secrete a large number of cytokines and chemokines either constitutively (e.g. (wikipedia.org)

Then, we used atorvastatin-stimulated cells or conditioned media from them in the Matrigel plug in vivo angiogenic assay. (hindawi.com)
Sequencing and alignment of this region in 11 cancer cell lines and 4 normal cell lines were also performed, followed by cloning, mutation and luciferase assay analysis. (jcancer.org)

These effects of diabetic plasma on enzyme activity and cell adhesion, mediated by PKCβ2-dependent phosphorylation of the core 2 GlcNAc-T protein, were found to be triggered by increased plasma levels of tumor necrosis factor (TNF)-α. (diabetesjournals.org)
Furthermore, we noted much higher correlation, as compared with control subjects, between increased values of core 2 GlcNAc-T activity and cell adhesion properties. (diabetesjournals.org)

As expected, CD19 protein is not detected in either U-937 cells or Raw 264.7 cells. (cellsignal.com)

B ) Levels of SAMHD1, phosphorylated SAMHD1, CDK1 and CDK2 proteins in activated or unstimulated purified CD4+ T cells treated with carrier, AhR agonist or antagonist. (elifesciences.org)
Those results also showed that the RB1 pathway inhibitors CDK2-IN-73 and Palbociclib could relieve the resistance of apatinib resistant cells. (bvsalud.org)

Mass-tag cell barcoding (MCB) labels individual cell samples with unique combinatorial barcodes, after which they are pooled for processing and measurement as a single multiplexed sample. (nature.com)
Figure 7: Single-cell deconvolution versus Boolean gating for MCB samples with and without large differences in MCB labeling intensity. (nature.com)
The histiocytic cell line U937 was found to produce IL-1 in response to environmental samples and it may be useful in the evaluation of the inflammatory potential of such samples. (cdc.gov)
In the second experiment the cell samples were submitted to one of the following treatment methods: 1. (emf-portal.org)

TNFα and recombinant GM-CSF for example independently promote IL-10 production in U937 cells. (wikipedia.org)

A. nilotica pod extract drug effectiveness on U937 cells by WST-1 for cytotoxicity. (phcogres.com)
Therefore, this study determined the cytotoxicity of KSO-SFE on colon cancer cells. (hindawi.com)

U-937 cells bear the t(10;11)(p13;q14) translocation causing a fusion of MLLT10 and Ap-3-like clathrin assembly protein PICALM. (wikipedia.org)
GA-binding protein and p300 are essential components of a retinoic acid-induced enhanceosome in myeloid cells. (umassmed.edu)
Immunoprecipitation of Syk protein from SR cell extracts, using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Syk(D3Z1E) XP ® Rabbit mAb (lane 3). (cellsignal.com)
This leads to an almost complete arrest of protein synthesis in the cell and its death. (org.ua)
On the other hand, DT represents a very interesting molecular machine which allows using this protein in different bioengineering applications, such as ablation of certain cell types in transgenic mice, development of immunotoxins targeted for specific cell markers and also for delivery of certain proteins into the cytosol. (org.ua)
We found that the cell cycle related protein RB1 may play an essential role in the process of apatinib resistant to hepatocarcinoma. (bvsalud.org)

mechanistically, rotenone-induced destruction of dopaminergic neurons has been attributed to its inhibition of the activity of neuronal mitochondrial complex I. However, the role of microglia, the resident brain immune cells in rotenone-induced neurodegeneration, has not been reported. (jneurosci.org)

THP-1 cells can also restore the response of purified T lymphocytes to Concanavlin A, show increased CO 2 production on phagocytosis and can be differentiated into macrophage-like cells using for example DMSO. (sigmaaldrich.com)
Ly6C low MC manifested activated T-cell signaling pathways and potentially can adapt the function of lymphocytes. (frontiersin.org)
The LLNA has been evaluated extensively in the context nocytes, Langerhans cells, and T-lymphocytes, have been of both national and international interlaboratory trials. (cdc.gov)

Flow cytometric analysis on U-937 cells. (bdbiosciences.com)
Flow cytometric analysis of Ramos cells, untreated (blue) or treated with anti-IgM (green), using Phospho-Syk(Tyr525/526) (C87C1) Rabbit mAb, or a concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (dashed lines). (cellsignal.com)

THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. (sigmaaldrich.com)
Infectious virus titres increased in undifferentiated U937 cells following infection with clinical isolates but only when the cells were infected via their Fc receptors. (microbiologyresearch.org)

Activated Akt inhibited GSK3 and prevented proteasome-mediated cyclin D1 degradation in LeTx-intoxicated THP-1 cells. (uwo.ca)

The disruption of the mucosal barrier increases the risk for HIV access to the bloodstream and inflammatory cells and serves as a focus for bacterial and viral shedding. (medscape.com)

We performed a time course analysis of cGAMP- and DNA-activated IFN responses, comparing control U937 cells to STING-deficient cells. (woofahs.com)

Cancer cells often maintain high basal level of ROS and are vulnerable to any further increase in ROS levels beyond a certain protective threshold. (degruyter.com)
Consequently, ROS-modulation has emerged as an anticancer strategy with synthesis of various ROS-inducing or responsive agents that target cancer cells. (degruyter.com)
This mini review highlights the development of compounds that target cancer cells by taking advantage of redox or metabolic alteration in cancer cells. (degruyter.com)
Cancer cells also enhance glucose-uptake to fuel these enhanced metabolic activities [ 2 ]. (degruyter.com)
High abundance of glucose in the cytoplasm of a cancer cell also increases flux into other metabolic pathways such as hexosamine biosynthetic pathway (HBP) [ 7 ]. (degruyter.com)
Redirection of glutamine to cellular energetics destabilizes glutathione homeostasis in glioblastoma cells and other cancer cells [ 9 ]. (degruyter.com)
This is because cancer cells maintain high basal level of ROS, thus they are vulnerable to any further increase in ROS (Fig. 1 ). (degruyter.com)
ROS-modulation in normal vs. cancer cell. (degruyter.com)
The IC50 values have been determined against cell line U937, the results obtained indicate the potential effects against cancer cell line. (niscair.res.in)
Compound 4f shows anticancer effect in cancer cell lines and in vivo that corresponds with antitumor activity in an AML cancer type. (niscair.res.in)
Sensitive ADAR editing reporter in cancer cells enables high-throughput screening of small molecule libraries. (cancerindex.org)
Stably introduced into cancer cell lines, the system reports on elevated endogenous ADAR1 editing activity induced by interferon as well as knockdown of ADAR1 and ADAR2. (cancerindex.org)
There were three nucleotide variants (T471C/A600G/C862T) frequently detected in cancer cell lines. (jcancer.org)
The site variation rates of T471C/A600G/C862T were 72.7%, 18.2%, and 72.7% in cancer cells respectively. (jcancer.org)
Hypoxia is the most common characteristic in the microenvironment of solid tumors because tumor progression and metastasis depend on the ability of cancer cells to initiate angiogenesis and ensure the delivery of oxygen and nutrients ( 11 , 12 ). (jcancer.org)
Taken together, our results clarify the suppressive role of SH3BGRL in tumorigenesis of liver cancer, which would be of help to the diagnosis of liver cancer, while either promoting the autophagy of liver cancer cells or inhibiting the downstream signaling induced from SH3BGRL downregulation would be a promising therapy. (bvsalud.org)

Western blot analysis of extracts from various cell lines using CD19 (Intracellular Domain) (D4V4B) XP ® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). (cellsignal.com)

Below are the most recent publications written about "U937 Cells" by people in Profiles. (umassmed.edu)

The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. (cellsignal.com)
Once differentiated, cells were susceptible to infection either with virus alone or with virus-antibody complexes. (microbiologyresearch.org)

Ford C , 2023 , 'Cell-free DNA in plasma and ascites as a biomarker of bevacizumab response: A translational sub-study of the REZOLVE (ANZGOG-1101) clinical trial. (edu.au)

Beside the cell types mentioned above, U-937 cells can be differentiated into osteoclasts - cell subtype crucial for bone resorption and remodeling. (wikipedia.org)

Mol Cell Biol. (umassmed.edu)

This parallel detection of PB1-F2 and NP suggests that applied sensor chip technology may be amenable to an arrow immunosensor for simultaneous detection of all known influenza virus proteins in infected tissues and cells. (omicsonline.org)
Ubiquitin becomes activated through a series of complicated steps and forms an isopeptide bond to lysine residues of specific proteins within the cell. (doctorinternet.com)
These "ubiquitinated" proteins can be recognized and degraded by proteosomes or be transported to specific compartments within the cell. (doctorinternet.com)

During 2010-2015 our researches were aimed at studying the interaction of diphtheria toxin (DT) with mammalian cells, as well as functions of DT receptor, the precursor of heparin-binding epidermal growth factor-like growth factor proHB-EGF. (org.ua)

Retinoic acid inhibits the cytoproliferative response to weak 50Hz magnetic fields in neuroblastoma cells med. (emf-portal.org)

Remove medium and resuspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 20% serum. (sigmaaldrich.com)

U-937V is a variant cell line derived from the original U-937 cells, the difference being their increased susceptibility to the effects of TNFα. (wikipedia.org)
In this chapter, we will focus on IL-21's biological effects and signaling pathways as well as discuss the potential implications and applications of IL-21 in leukemia cells. (intechopen.com)
Effects of AhR activation in PBMC and purified CD4+ T cells. (elifesciences.org)

Resolution of these problems has been approached in Drosophila by carrying out an in vivo functional analysis of Drosophila Gap1 in the context of cone cell determination in the developing Drosophila eye. (sdbonline.org)
The cell potency of cell line is best for compounds 4a IC50 = 62.5 μM, 5b IC50 = 62.5 μM, 4b IC50 = 31.2 μM, 4e IC50 = 31.2 μM), selectivity and in vivo . (niscair.res.in)

Anatomy32

Organisms17

Diseases30

Chemicals and Drugs72

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Phenomena and Processes70

Disciplines and Occupations1

Information Science3

Health Care1

Monocytes11

Macrophages11

Histiocytic lymphoma2

Monocytic cell line2

Tumor Cells1

Mononuclear cells5

Apoptosis14

Jurkat1

Differentiation7

Proliferation10

Myeloid cell2

Acute3

Human29

Endothelial7

Flow cytometry2

Cytotoxic2

Progenitor Cells2

Expression6

Vitro2

Phagocytic1

Metabolism1

MeSH1

Phenotype2

Induces1

Dendritic cells1

Eukaryotic cells1

Ligands1

Cytometry2

Serum starved overnight1

Cytokines1

Assay2

Adhesion2

264.71

CDK22

Samples4

Recombinant1

Cytotoxicity2

Protein6

Immune cells1

Lymphocytes3

Cytometric analysis2

Receptors2

Degradation1

Inflammatory1

Responses1

Cancer16

Extracts1

Profiles1

Antibody2

20231

Subtype1

Biol1

Proteins3

Mammalian1

Retinoic1

Pellet1

Effects3

Vivo2