• These changes correlated with down-regulation of effectors of differentiation [secreted frizzled-related sequence protein 1 ( Sfrp1 ), insulin-like growth factor 1 receptor ( Igf1r ), wingless-related MMTV-integration site 4 ( Wnt4 ), Lim homeobox protein 1 ( Lhx1 ), E-cadherin]. (nih.gov)
  • C. elegans homeobox protein ceh-14. (embl.de)
  • C. elegans homeobox protein unc-97. (embl.de)
  • This gene encodes a LIM zinc-binding domain-containing protein that binds to actin filaments and mediates interactions between actin and cytoplasmic targets. (nih.gov)
  • The most ancient LIM-HD protein and its partnership with Ldb1 were innovated in the metazoan ancestor by gene fusion combining LIM domains and a homeodomain and by creating the LIM domain-interacting domain (LID) in ancestral Ldb, respectively. (nih.gov)
  • Cloning and expression of a novel human gene, Isl-2, encoded a LIM-homeodomain protein. (nih.gov)
  • This gene encodes a PDZ domain-containing protein. (antibodies-online.cn)
  • The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. (antibodies-online.cn)
  • The LIMK1 gene provides instructions for making a protein that is highly active in the brain, where it is thought to be involved in the growth and development of nerve cells. (medlineplus.gov)
  • Vertebrate insulin gene enhancer binding protein isl-1. (embl.de)
  • Isl-1 binds to one of the two cis-acting protein-binding domains of the insulin gene. (embl.de)
  • Lhx9 is a member of the LIM homeobox gene family. (nih.gov)
  • Indeed, researchers have identified individuals with hemizygosity limited to the gene for elastin, a structural protein found in large arteries and other elastic connective tissue, and have established that SV AS results from this deletion. (psychologicalscience.org)
  • Automated radiosynthesis of [F]FBEM, a sulfhydryl site specific labeling agent for peptides and proteins. (nih.gov)
  • Multiplexed absolute quantification in proteomics using artificial QCAT proteins of concatenated signature peptides. (genomeweb.com)
  • Peptide mass mapping is the matching of experimentally generated peptides masses with the predicted masses of digested proteins contained in a database. (koreamed.org)
  • In molecular biology, the LIM domain-binding protein family is a family of proteins which binds to the LIM domain of LIM (LIN-11, Isl-1 and MEC-3) homeodomain proteins which are transcriptional regulators of development. (wikipedia.org)
  • Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB1) is located in the nuclei of neuronal cells during development, it is co-expressed with ISL1 in early motor neuron differentiation and has a suggested role in the ISL1 dependent development of motor neurons. (wikipedia.org)
  • It is suggested that Chip cooperates with different LIM domain proteins and other factors to structurally support remote enhancer-promoter interactions. (wikipedia.org)
  • The LIM domain has multiple interacting interphases, and Ldb1 has a dimerization domain (DD), the LID, and other interacting domains that bind to Ssbp2/3/4 and the boundary factor, CTCF. (nih.gov)
  • The nuclear LIM domain interactor NLI mediates homo- and heterodimerization of LIM domain transcription factors. (nih.gov)
  • PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. (antibodies-online.cn)
  • A nuclear isoform of the focal adhesion LIM-domain protein Tri. (kit.edu)
  • LIM-only protein 4 (LMO4) and LIM-domain binding protein 1 (LDB1), transcriptional adaptors that have important roles in normal epithelial cell differentiation, have also been associated with increased lymph node metastasis and decreased differentiation in carcinomas of the breast and of the head and neck. (vanderbilt.edu)
  • LIM domain proteins all contain at least one double zinc-finger motif. (edu.au)
  • While several recent reviews have summarised the importance of individual LIM domain proteins, this is the first review of its kind to cover all LIMs associated with the heart. (edu.au)
  • Our analysis is consistent with the view that cardiac LIM domain proteins form multiple extensive networks of multi-protein complexes within the myocardium. (edu.au)
  • the E-value for the LIM domain shown below is 2.08e-12. (embl.de)
  • the E-value for the LIM domain shown below is 6e-18. (embl.de)
  • 10. Glucose induces pancreatic islet cell apoptosis that requires the BH3-only proteins Bim and Puma and multi-BH domain protein Bax. (nih.gov)
  • LIM domain only-2 (LMO2) induces T-cell leukemia by two distinct pathways. (nih.gov)
  • Therefore, an antibody cytokine fusion protein (scFv_RD_IL-15) composed of an antibody moiety targeting the tumor stromal fibroblast activation protein (FAP), an extended IL-15Rαsushi domain (RD) and IL-15 was generated, exhibiting antibody-mediated specific binding and cytokine activity in soluble and targeted form. (aacrjournals.org)
  • PDLIM4, also known as PDZ and LIM domain protein 4, has 2 isoforms, a 330 amino acid long isoform that is 35 kDa and a short 246 amino acid isoform that is 26 kDa and found in brain. (novusbio.com)
  • CRISPR/Cas9-mediated knockout of Lim-domain only four retards organ of Corti cell growth. (nih.gov)
  • Lim-domain only 4 (LMO4) plays a critical role in mediating the ototoxic side-effects of cisplatin, a highly effective anti-cancer drug. (nih.gov)
  • Many of the proteins in this class are involved in intracellular signaling processes and mediate their effects via LIM domain protein-protein interactions. (bvsalud.org)
  • This entry represents LIM-type zinc finger (Znf) domains. (embl.de)
  • Zinc finger (Znf) domains are relatively small protein motifs which contain multiple finger-like protrusions that make tandem contacts with their target molecule. (embl.de)
  • A large class of structurally-related proteins that contain one or more LIM zinc finger domains. (bvsalud.org)
  • Davila M, Frost AR, Grizzle WE, Chakrabarti R. LIM kinase 1 is essential for the invasive growth of prostate epithelial cells: implications in prostate cancer. (medlineplus.gov)
  • Davila M, Jhala D, Ghosh D, Grizzle WE, Chakrabarti R. Expression of LIM kinase 1 is associated with reversible G1/S phase arrest, chromosomal instability and prostate cancer. (medlineplus.gov)
  • 9. Protein kinase Cδ regulates nuclear export of FOXO1 through phosphorylation of the chaperone 14-3-3ζ. (nih.gov)
  • To investigate the possible roles of LAMMER kinase homologue, Lkh1, in Schizosaccharomyces pombe, whole proteins were extracted from wild type and lkh1-deletion mutant cells and subjected to polyacrylamide gel electrophoresis. (koreamed.org)
  • It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors both of which have essential roles in development. (wikipedia.org)
  • Mammalian LH-2, a transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types. (embl.de)
  • Lim1/Lhx1 is a member of the LIM homeodomain (LIM-HD) protein family, and its intimate partner, Ldb1/NLI, binds to two tandem LIM domains of LIM-HDs. (nih.gov)
  • 2020 ). The intracellular levels of individual proteins are adjusted to the particular needs and status of every single cell in the organism (Jayaraj et al. (springer.com)
  • This antibody is purified through a protein A column, followed by peptide affinity purification. (antibodies-online.cn)
  • Targeted approaches by means of antibody-cytokine fusion proteins might enable focus on the cytokine activity to the tumor site, thereby reducing unwanted side effects. (aacrjournals.org)
  • Comparative analysis with a corresponding antibody fusion protein devoid of RD (scFv_IL-15) showed for scFv_RD_IL-15 in solution enhanced stimulatory activity on Mo7e (IL-15Rβγ) cells and reduced proliferation response on CTLL-2 (IL-15Rαβγ) cells, while in FAP-targeted, that is, membrane-bound form, comparable proliferation of CTLL-2 (IL-15Rαβγ) cells was obtained. (aacrjournals.org)
  • Thus, tumor-directed trans -presentation of IL-15 in association with RD in form of an antibody fusion protein seems to be a promising approach to further improve the antitumor effect of IL-15. (aacrjournals.org)
  • These genes appear to encode two major subgroups of proteins, cytoskeletal and sarcomeric proteins. (medscape.com)
  • Cite this: Dilated Cardiomyopathy: A Tale of Cytoskeletal Proteins and Beyond - Medscape - Aug 01, 2006. (medscape.com)
  • Vertebrate paxillin, a cytoskeletal focal adhesion protein. (embl.de)
  • Our proteomic analysis provides essential new insights in the composition of pathological protein aggregates in skeletal muscle fibers of desminopathy patients. (nih.gov)
  • Protein post-translational modifications (PTMs) can regulate protein activity and localization as well as protein-protein interactions in numerous cellular processes, thus leading to elaborate regulation of plant responses to various external stimuli. (mdpi.com)
  • We believe that the complexity of LIM interactions is properly described by the term LIM interactome. (edu.au)
  • 14. alpha3beta1 integrin promotes cell survival via multiple interactions between 14-3-3 isoforms and proapoptotic proteins. (nih.gov)
  • The lipid droplet-associated protein ABHD5 protects the heart through proteolysis of HDAC4. (uni-heidelberg.de)
  • Alters lipid metabolism by interacting with hepatocellular proteins involved in lipid accumulation and storage (PubMed:14602201). (proteopedia.org)
  • PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. (antibodies-online.cn)
  • Counter to the commonly held notion that methionines (which are hydrophobic residues) are typically buried within proteins, many methionine residues lie on the surfaces of proteins. (nih.gov)
  • Three mutant desmin variants were detected directly on the protein level as components of the aggregates, suggesting their direct involvement in aggregate-formation and demonstrating for the first time that proteomic analysis can be used for direct identification of a disease-causing mutation in myofibrillar myopathy. (nih.gov)
  • The bar plot below shows the proportion of tumor samples that have any kind of altering mutation(s) in the given protein. (phosphosite.org)
  • Here within, LMO4 and LDB1, in addition to single-stranded-binding proteins (SSBPs) are involved in regulating invasion, and provides an explanation for why LMO4 and LDB1 have concordant levels of expression in this disease. (vanderbilt.edu)
  • Mammalian cysteine-rich intestinal protein (CRIP), a small protein which seems to have a role in zinc absorption and may function as an intracellular zinc transport protein. (embl.de)
  • Several years ago, in collaboration with the late Earl Stadtman, Dr. Levine discovered a novel form of protein modification-carbonylation-that is now a widely accepted measure of oxidative modification in cells ranging from bacteria to humans. (nih.gov)
  • A census of actin-associated proteins in humans. (ncbs.res.in)
  • Mammalian and avian cysteine-rich protein (CRP), a 192 amino-acid protein of unknown function. (embl.de)
  • The proteome of a mammalian cell contains thousands of distinct proteins (Jayaraj et al. (springer.com)
  • Within cells, the LIMK1 protein likely regulates aspects of the cytoskeleton, the structural framework that helps to determine cell shape, size, and movement. (medlineplus.gov)
  • Protein structural dynamics in solution unveiled via 100-ps time-resolved x-ray scattering. (nih.gov)
  • Tracking the structural dynamics of proteins in solution using time-resolved wide-angle X-ray scattering. (nih.gov)
  • SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
  • Participates in the viral particle production as a result of its interaction with the non-structural protein 5A (By similarity). (proteopedia.org)
  • Owing to the generation of vast amounts of sequencing data by using cost-effective, high-throughput sequencing technologies with improved computational approaches, many putative proteins have been discovered after assembly and structural. (koreamed.org)
  • Structural information has been a major concern for biological and pharmaceutical studies for its intimate relationship to the function of a protein. (koreamed.org)
  • It is made up of a network of lymphatic vessels that carry lymph - a clear, watery fluid that contains proteins, salts, and other substances - throughout the body. (kidshealth.org)
  • Vertebrate homeobox proteins lim-1, lim-2 (lim-5) and lim3. (embl.de)
  • Rhombotin 1 (RBTN1 or TTG-1) and rhombotin-2 (RBTN2 or TTG-2) are proteins of about 160 amino acids whose genes are disrupted by chromosomal translocations in T-cell leukemia. (embl.de)
  • Relationship Between Cardiac Fibroblast Activation Protein Activity by Positron Emission Tomography and Cardiovascular Disease. (uni-heidelberg.de)
  • In different pathologies of the heart a nuclear location of MLP, which was so far known primarily as a cytosceletal protein, has been observed. (uni-goettingen.de)
  • These proteins contain between one and five LIM domains and usually these proteins contain other domains that have specific functions such as actin-binding, kinases and nuclear translocation motifs. (edu.au)
  • Nuclear translocation triggers proteolytic degradation of AHR protein, a signaling event that defines the biological responsiveness to AHR ligands ( Roberts and Whitelaw 1999 ). (nih.gov)
  • 5. Involvement of the RNA-binding protein ARE/poly(U)-binding factor 1 (AUF1) in the cytotoxic effects of proinflammatory cytokines on pancreatic beta cells. (nih.gov)
  • Sample: Recombinant protein. (biomatik.com)
  • Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)
  • In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
  • A method for the isolation of covalent DNA-protein crosslinks suitable for proteomics analysis. (genomeweb.com)
  • Ascribing subcellular localization of proteins assists understanding function and has largely been addressed through 'omic' approaches, such as proteomics of purified organelles and hyperplexed organelle localizations by isotope tagging 1 . (nature.com)
  • Here, using fluorescence microscopy and cell lines expressing endogenously tagged proteins, we mapped the subcellular localization of 89% of the T. brucei proteome, a resource we call TrypTag. (nature.com)
  • His work has yielded several highly cited publications dealing with signal transduction molecules downstream of the TCR, like LAT and RLK, and surface proteins that regulate TCR signaling, like CD5. (nih.gov)
  • These modifications serve to control oxidative stress, regulate cellular function, and modulate protein structure. (nih.gov)
  • LIM domains coordinate one or more zinc atoms, and are named after the three proteins (LIN-11, Isl1 and MEC-3) in which they were first found. (embl.de)
  • El nombre LIM se deriva de las tres primeras proteínas en las cuales el motivo se a encontrado: LIN-11, Isl1 y Mec-3. (bvsalud.org)
  • The name LIM is derived from the first three proteins in which the motif was found: LIN-11, Isl1 and Mec-3. (bvsalud.org)
  • Although the proteins involved in these diseases are different, they have in common the accumulation of pathological protein inclusions in neurons. (springer.com)
  • Actin filaments are necessary for several normal cellular functions, such as cell division, cell movement (motility), maintenance of cell shape, transport of proteins and other molecules within cells, and chemical signaling between cells. (medlineplus.gov)
  • Watching a protein as it functions with 150-ps time-resolved x-ray crystallography. (nih.gov)
  • They display considerable versatility in binding modes, even between members of the same class (e.g. some bind DNA, others protein), suggesting that Znf motifs are stable scaffolds that have evolved specialised functions. (embl.de)
  • Understanding their complex cell architecture and life cycle is challenging because, as with most eukaryotic microbes, ~50% of genome-encoded proteins have completely unknown functions. (nature.com)
  • 6. Modulation of Bcl-2-related protein expression in pancreatic beta cells by pro-inflammatory cytokines and its dependence on the antioxidative defense status. (nih.gov)
  • Protein expression profiling identifies maspin and stathmin as potential biomarkers of adenoid cystic carcinoma of the salivary glands. (genomeweb.com)
  • Glucose stimulated the expression of TXNIP mRNA and protein in a concentration and time-dependent manner. (cdc.gov)
  • Treatment of macrophages with MWCNTs stimulated the expression of TXNIP at mRNA and protein levels compared to vehicle control. (cdc.gov)
  • Here we examine 33 LIM proteins (including three that bind to, but do not themselves contain, LIM domains) that are implicated in either the development of the heart, heart disorders and failure, or both. (edu.au)
  • Some LIM domains bind protein partners via tyrosine-containing motifs. (embl.de)
  • LIM domains are found in many key regulators of developmental pathways. (embl.de)
  • In vivo functional mapping of the conserved protein domains within murine Themis1. (nih.gov)
  • Computational analysis of the flexibility in the disordered linker region connecting LIM domains in cysteine-glycine-rich protein. (ncbs.res.in)
  • Clinical experience indicates that enhanced level of heat shock protein 70 (Hsp70) and p53 correlates with poor prognosis due to malignant cell overexpression of these proteins in tumor progression. (intechopen.com)
  • Visualizing reaction pathways in photoactive yellow protein from nanoseconds to seconds. (nih.gov)
  • Thus, modulation of protein clearance pathways is a promising approach for therapeutics. (springer.com)
  • Misfolded proteins that ensue from external and internal stressors are degraded through two major protein clearance pathways, i.e., the ubiquitin-proteasome system (UPS) and the autophagy-lysosome pathway. (springer.com)
  • Actin-Binding LIM 1 (ABLIM1) Inhibits Glioblastoma Progression and Serves as a Novel Prognostic Biomarker. (nih.gov)
  • The LIMK1 protein helps control the organization of actin filaments, which are long, thin fibers that are part of the cytoskeleton. (medlineplus.gov)
  • Researchers believe that high levels of the LIMK1 protein may be associated with changes in the organization of actin filaments and an increased chance that a tumor will invade other tissues. (medlineplus.gov)
  • Furthermore, in vivo analysis in a lung metastasis tumor mouse model revealed a superior antitumor effect for scFv_RD_IL-15 in comparison with that obtained by an untargeted or RD missing version of IL-15 fusion protein. (aacrjournals.org)
  • In eukaryotic cells, DNA is packaged into chromatin, a dynamic structure containing genomic DNA, histones, and nonhistone proteins. (nature.com)
  • Although reactive oxygen species are usually promiscuous in their reactivity, Dr. Levine and his colleagues found that this modification was localized to the metal binding sites of proteins that contained them. (nih.gov)
  • Pattern finding is one of the important tasks in a protein or DNA sequence analysis. (koreamed.org)
  • The spleen has special white blood cells called lymphocytes (LIM-fuh-sites) and macrophages (MA-kruh-fages). (kidshealth.org)
  • Induction of thioredoxin-interacting protein and role in NLRP3 activation by carbon nanotube s in macrophages. (cdc.gov)
  • Taken together, these findings indicate that TXNIP is a glucose-dependent regulatory protein and is induced in MWCNT-activated macrophages where it may modulate proinflammatory responses through NLRP3-mediated, caspase 1-dependent production of IL-1beta and other proinflammatory mediators. (cdc.gov)
  • Three-dimensional representation of the positions of protein atoms. (koreamed.org)
  • However, such localization attributions are limited by the accuracy of organelle purification or fractionation, and sensitivity is limited by protein abundance and characteristics. (nature.com)
  • Crystallization techniques for both small molecules and proteins. (nottingham.ac.uk)
  • Desminopathy is a subtype of myofibrillar myopathy caused by desmin mutations and characterized by protein aggregates accumulating in muscle fibers. (nih.gov)
  • The aim of this study was to assess the protein composition of these aggregates. (nih.gov)