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  • ligase
  • 1-3 The QuickLink™ DNA Ligation Kit has been optimized for utilization of T4 DNA ligase for rapid, efficient blunt and cohesive ligations performed at room temperature. (sigmaaldrich.com)
  • If the total volume of DNA is greater than 10 µl, the volume of the ligation buffer and the ligase should be adjusted accordingly, and the incubation time should be extended to 20-30 minutes. (sigmaaldrich.com)
  • vector
  • There are many variables in ligation (e.g. concentration and size of DNA, vector/insert ratio etc.), and this is the bit I'd confess - I tend to be a bit slapdash in what I do and just ignore the variables. (bio.net)
  • It's probably not the ligation that is the problem but the capacity of the E.coli for such a high copy vector with that size of insert. (bio.net)
  • When performing a vector + insert ligation, it is recommended to dephosphorylate the vector DNA to minimize vector recircularization. (sigmaaldrich.com)
  • Previous
  • With the introduction in the 1970s of laparoscopy (the so-called "belly button surgery"), which was less invasive than previous surgeries, more women than ever before chose tubal ligation, and reports of postoperative symptoms increased, says Corson. (medicinenet.com)
  • If you have diabetes or a history of previous abdominal surgery, pelvic inflammatory disease, or lung disease, or are overweight, you may have a higher risk for problems after tubal ligation. (stanfordchildrens.org)
  • plasmid
  • I suspect that the problem is with the gel purification and not with my digestions for the following reason: If I just do the digestion followed by the phosphatase (but without any gel purification), the kinase control works perfectly (the plasmid can recircularize), but unfortunately the negative control (ligation without kinase) produces approx. (bio.net)
  • possibility
  • In the protocol given in Molecular Cloning, they suggest using PEG or another chemical (didn't have it available) to increase the possibility of ligation. (bio.net)
  • abdomen
  • ive ad a tubal ligation done 2 years ago wid the clips and over the past 3 weeks ive been getting really tired, sore breasts, moody, bad back, heartburn and fluttering in abdomen, constipation etc.... i ad a few days 2 weeks ago where i was on a period but it was lighter and shorter than normal, i also av vaginal discharge more. (medhelp.org)
  • birth control
  • According to Corson and other doctors looking into post-tubal ligation "syndrome," it may actually be a combination of discontinuing the use of birth control pills and age-related factors. (medicinenet.com)