Cell surface proteins tagged with a 15-amino acid peptide are biotinylated by Escherichia colibiotin ligase (BirA), whereas endogenous proteins are not modified. (ox.ac.uk)
however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. (thermofisher.com)
I adapted the biotin ligase (BirA)-based proximity labeling method for polarity proteins in the stomatal lineage and identified the respective plasma membrane proteomes. (oeaw.ac.at)
Biotin, also known as vitamin B7, is a chemical moiety that can be post-tranlationally added to proteins via a process known as biotinylation. (cellsignal.com)
One advantage is the very strong binding interaction with avidin that can be used to purify biotin-tagged proteins (2,3). (cellsignal.com)
These new cellular assays utilize biotin ligase or engineered ascorbate peroxidase (APEX) fusion proteins that are able to biotinylate nearby protein targets within specific cellular compartments. (cellsignal.com)
Biotinylated NEDD8 is activated by NEDD8 E1, is a competent substrate for the NEDD8 E2 conjugating enzyme UbcH12 and can be conjugated to target proteins in the presence of a suitable NEDD8 E3 ligase. (enzolifesciences.com)
Conjugate1
Protein L Biotin Conjugate is a high quality a biotin conjugate of Protein L, a cell wall molecule from the bacterial species Peptostreptococcus magnus. (moleculardepot.com)
Escherichia1
1: Maeda Y, Yoshino T, Matsunaga T. In vivo biotinylation of bacterial magnetic particles by a truncated form of Escherichia colibiotin ligase and biotin acceptor peptide Appl Environ Microbiol. (prlog.org)
Ubiquitin-protei2
p53 E3 ubiquitin protein ligase homolog (Mdm2) is an important negative regulator of p53. (molvis.org)
E6 associates with host E6-AP ubiquitin-protein ligase, and inactivates tumor suppressors TP53 and TP73 by targeting them to the 26S proteasome for degradation. (joplink.net)
Peptide5
Definition of Activity: 1 Unit is the amount of enzyme that will biotinylate 1 pmol of AviTag peptide substrate in 30 minutes at 30°C in the reaction buffer containing 10 mM Tris-HCl, pH 8.0, 10 mM ATP, 10 mM MgOAc, 50 µM d-Biotin, and 38 µM peptide substrate. (amidbiosciences.com)
To create the probes, the researchers used the enzyme biotin ligase and its target, a 12-amino-acid peptide. (medicalxpress.com)
In this study, the researchers applied the two aforementioned strategies: tandem affinity purification with the SFB ( S-protein , FLAG epitope, and streptavidin-binding peptide) tag, as well as proximity labeling by using a second-generation biotin ligase, BioID2. (news-medical.net)
YgiC and YjfC Possess Peptide─Spermidine Ligase Activity. (edu.au)
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. (cusabio.com)
Protein5
Inhibitors of biotin protein ligase (BPL) are novel antimicrobial compounds with the potential to treat infections caused by bacteria resistant to current antibiotics. (elsevierpure.com)
Bisubstrate adenylation inhibitors of biotin protein ligase from Mycobacterium tuberculosis. (cornell.edu)
Bisubstrate Inhibitors of Biotin Protein Ligase in Mycobacterium tuberculosis Resistant to Cyclonucleoside Formation. (cornell.edu)
2015. Targeting Mycobacterium tuberculosis Biotin Protein Ligase (MtBPL) with Nucleoside-Based Bisubstrate Adenylation Inhibitors. . (cornell.edu)
This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag. (cusabio.com)
In enzymology, a biotin-CoA ligase (EC 6.2.1.11) is an enzyme that catalyzes the chemical reaction ATP + biotin + CoA ⇌ {\displaystyle \rightleftharpoons } AMP + diphosphate + biotinyl-CoA The 3 substrates of this enzyme are ATP, biotin, and CoA, whereas its 3 products are AMP, diphosphate, and biotinyl-CoA. (wikipedia.org)
This enzyme belongs to the family of ligases, specifically those forming carbon-sulfur bonds as acid-thiol ligases. (wikipedia.org)
The systematic name of this enzyme class is biotin:CoA ligase (AMP-forming). (wikipedia.org)
We also offer solutions for automation, site-directed mutagenesis, as well as your favorite restriction enzyme, ligase or competent cell products. (neb.com)
Structure-Based Optimization of Pyridoxal 5'-Phosphate-Dependent Transaminase Enzyme (BioA) Inhibitors that Target Biotin Biosynthesis in Mycobacterium tuberculosis. (cornell.edu)
Thermo Fisher Sci1
The following product was used in this experiment: Parkin Polyclonal Antibody, Biotin from Thermo Fisher Scientific, catalog # PARK-BIOTIN. (thermofisher.com)
Metabolism1
Pacheco-Alvarez D, Solorzano-Vargas RS, Del Rio AL: Biotin in metabolism and its relationship to human disease. (smpdb.ca)
Polyclonal antibodies1
Side by side comparison of two commercially available anti-biotin polyclonal antibodies with Cell Signaling Technology's anti-biotin (A7C2A) rabbit monoclonal antibody. (cellsignal.com)
Inhibitors1
2015. Target-based identification of whole-cell active inhibitors of biotin biosynthesis in Mycobacterium tuberculosis. . (cornell.edu)
Coli1
This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Buffer, 0.170 ml of 100 x Biotin, 0.170 ml of 100 x hydroxylamine, 0.170 ml of 100 x ATP, 0.170 ml of 100 x E. coli BirA (5000 nM), 0.170 ml of 100 x pyrophosphatase (PPase, 10 U/ml) and 25 ml of Dye for phosphate detection. (profoldin.com)
Proximity3
This type of biotin proximity assay can provide improved information about subcellular localization, multi-subunit components, and recruitment of unknown targets. (cellsignal.com)
This assay provides rich data for these types of biotin proximity assays such as the APEX (6,7) engineered cell system and other related methods. (cellsignal.com)
Here we have deployed proximity labeling using the engineered biotin ligase TurboID (TbID) and quantitative proteomics to compare the neighborhoods of emerin and LBR in cultured mouse embryonic fibroblasts (MEFs). (biorxiv.org)
Antibody2
E6 Antibody, Biotin conjugated is Available at Gentaur Genprice with the fastest delivery. (joplink.net)
CusabioE6 Antibody, Biotin conjugated is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send. (joplink.net)
Synthetase1
Other names in common use include biotinyl-CoA synthetase, biotin CoA synthetase, and biotinyl coenzyme A synthetase. (wikipedia.org)
20231
SAN DIEGO - May 7, 2023 - PRLog -- Magnetic Particles Conjugated to Biotin (80-140 nm) is a high quality superparamagnetic particles (80-140 nm) conjugated to Biotin. (prlog.org)
Gene1
2011. Evaluating the sensitivity of Mycobacterium tuberculosis to biotin deprivation using regulated gene expression. . (cornell.edu)
Affinity1
Monovalent streptavidin has only a single functional biotin-binding site, but retains the femtomolar affinity, low off-rate and high thermostability of wild-type streptavidin. (ox.ac.uk)
Modification1
6: Weiss B, Schneider M, Muys L, Taetz S, Neumann D, Schaefer UF, Lehr CM. Coupling of biotin-(poly(ethylene glycol))amine to poly(D,L-lactide-co-glycolide) nanoparticles for versatile surface modification Bioconjug Chem. (moleculardepot.com)
Method1
We have established a method allowing to study the cell-specific secretome of pericytes in vitro-using Turbo-ID, a mutant non-toxic biotin ligase (6). (lu.se)