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  • fundamental role
  • Collectively, these data suggest that both cadherins and integrins play a fundamental role in mediating myogenic constriction but argue against their direct involvement in mediating pressure-induced [Ca 2+ ]i increases. (frontiersin.org)
  • changes
  • The purpose of this study was to investigate: (1) whether cadherin inhibition blocks myogenic responses to increases in intraluminal pressure and (2) the effect of the cadherin or integrin blockade on pressure-induced changes in [Ca 2+ ]i. (frontiersin.org)
  • peptide
  • Using peptide array technology, we identified set of overlapping peptides derived from the soluble CD23 sequence that interact with integrins expressed on the surface of monocytic cells and with purified αVβ3 and αVβ5 integrins in in vitro Biacore assays. (bl.uk)
  • Whole-cell, inward Ba 2+ currents were inhibited after application of soluble cyclic RGD peptide, vitronectin (VN), fibronectin (FN), either of two anti-β 3 integrin antibodies, or monovalent β 3 antibody. (rupress.org)
  • Integrin Ligands with α/β-Hybrid Peptide Structure: Design, Bioactivity, and Conformational Aspects. (uni-bielefeld.de)
  • Conformational control of integrin-subtype selectivity in isoDGR peptide motifs: a biological switch. (uni-bielefeld.de)
  • Synthesis, SAR and in vitro evaluation of new cyclic Arg-Gly-Asp pseudopentapeptides containing a s-cis peptide bond as integrin alphavbeta3 and alphavbeta5 ligands. (uni-bielefeld.de)
  • The crystal structure of a complex between the I domain of integrin alpha2beta1 and a triple helical collagen peptide containing a critical GFOGER motif has been determined. (sdbonline.org)
  • subunit
  • Cytokine production by CD23-binding integrins appears to require the ligation of the α subunit of the integrin heterodimer. (bl.uk)
  • To directly examine the role of this integrin in cell migration, we used keratinocytes derived from wild-type mice or mice expressing a null mutation in the beta6 subunit (beta6-/-) to perform migration assays in vitro. (biologists.org)
  • mediate
  • Our work suggests that activated integrins are actively ordered at the molecular scale by cellular forces, which may underlie their ability to sense directional forces in their environment to mediate critical functions. (pnas.org)
  • Cytoskeletal forces in migrating cells generated by polymerization- or contractility-driven "retrograde flow" of F-actin from the cell leading edge have been hypothesized to mediate integrin activation for ligand binding. (pnas.org)
  • novel
  • The RKC motif can be considered a novel recognition motif for integrins, as it is cation-independent, and its binding is not blocked by the presence of RGD-containing integrin ligands. (bl.uk)
  • In this study, we introduce XCL1/lymphotactin, a member of the chemokine family, as a novel ligand for α9 integrin. (jimmunol.org)
  • In this study, we demonstrate that XCL1 functions in autoimmune diseases as a novel α9 integrin ligand. (jimmunol.org)
  • Helicobacter pylori type IV secretion apparatus exploits beta1 integrin in a novel RGD-independent manner. (uni-bielefeld.de)
  • epitope
  • However, within a particular family, cytokine production induced by integrin ligation was specific and relied on the recognition of a precise epitope on the integrin. (bl.uk)
  • pathways
  • The data suggest that α v β 3 and α 5 β 1 integrins are differentially linked through intracellular signaling pathways to the L-type Ca 2+ channel and thereby alter control of Ca 2+ influx in vascular smooth muscle. (rupress.org)
  • Thus, RGD peptides may be capable of directly stimulating integrin-dependent intracellular signaling pathways. (rupress.org)
  • The modulation of neutrophil activation through integrin-mediated pathways is important in the homeostatic control of the resolution of inflammatory states. (nih.gov)
  • peptides
  • Substitution of the residues in the RKC motif reduced or abolished binding of the peptides to integrins expressed on cells and in vitro, as measured by Biacore analysis, and abolished the competition with CD23. (bl.uk)
  • One important, unresolved issue is whether RGD peptides act solely by disrupting cell-ECM contacts or whether they provide direct signals to cells by binding to unoccupied integrins. (rupress.org)
  • cellular
  • These results emphasize the central role of the common beta 1 chain in regulating different adhesive functions mediated by VLA integrins as well as cellular morphology. (rupress.org)
  • Blocking αVβ3 integrin occupancy results in attenuation of the cellular migration response to insulin-like growth factor I (IGF-I). To determine whether integrin antagonists alter other IGF-I-stimulated biologic actions, quiescent smooth muscle cells (SMCs) were exposed to echistatin and their ability to respond to IGF-I was determined. (pnas.org)
  • The second β 3 integrin, α v β 3 , has a wide cellular and ligand distribution, and is involved in several important biological functions such as angiogenesis. (bmj.com)
  • motif
  • Taken together, these data suggest that the RKC motif is the site in CD23 that is recognised and bound by αVβ3 and αVβ5 integrins. (bl.uk)
  • Mutagenesis studies have shown that the MIDAS motif and exposed side chains on the surrounding surface are required for ligand binding, and are thus likely to form the ligand contact sites. (sdbonline.org)
  • focal
  • We used polarization-based microscopy techniques to discover that activated αVβ3 integrins are aligned with one another in focal adhesions in migrating fibroblasts. (pnas.org)
  • antigen
  • Once leukocytes are guided to sites of infection, inflammation, or antigen presentation, integrins can participate in the initiation, maintenance, or termination of the immune and inflammatory responses. (nih.gov)
  • bound
  • The initial ligand concentration also determines the probability of binding since it allows for more ligand to be bound to the protein binding sites. (physicsforums.com)
  • The scatchard equation is (r/c) = Ka*n - Ka*r, where r is the ratio of the concentration of bound ligand to total available binding sites, c is the concentration of free ligand, Ka is the association constant, and n is the number of binding sites per protein, right? (physicsforums.com)
  • So through this I could find the ratio of bound ligand to total available binding sites under the given ligand concentration and Kd (the inverse would be Ka). (physicsforums.com)
  • critical
  • In addition, during recirculation, T lymphocyte movement through distinct microenvironments is mediated by integrins, which are critical for cell cycle, differentiation and gene expression. (nih.gov)
  • The β₃-integrin ligand of Borrelia burgdorferi is critical for infection of mice but not ticks. (mcw.edu)
  • Critical to the ligand-binding functions of the α M I domain is the metal ion-dependent adhesion site (MIDAS). (rupress.org)
  • cytoskeleton
  • Integrin coalignment is sensitive to mechanical resistance of its ligand and coupling to a dynamic F-actin cytoskeleton, consistent with the "cytoskeleton force model" for integrin activation. (pnas.org)
  • leukocytes
  • Disintegrins have proven useful as tools to improve the understanding of the molecular events regulated by integrin signaling in leukocytes and prototypes in order to design therapies able to interfere with integrin-mediated effects. (nih.gov)
  • For example, the carbohydrate ligand for P-selectin, P-selectin glycoprotein ligand-1 (PSGL-1), is expressed by different types of leukocytes (white blood cells). (wikipedia.org)
  • concentration
  • The cytokine profile induced by integrin ligation did not differ between the αV and β2 integrin families, although the concentration of cytokines produced varied depending on the heterodimer targeted. (bl.uk)
  • activate
  • We conclude that occupancy of the αVβ3 integrin is necessary for IGF-I to fully activate the kinase activity of the IGF-I receptor and phosphorylate IRS-1. (pnas.org)
  • known
  • In contrast, exposure of SMCs to vitronectin (1.0 μg/cm 2 ) or thrombospondin (0.25 μg/cm 2 ), two known ligands for αVβ3, resulted in enhancement of the IGF-I-stimulated IRS-1 response. (pnas.org)
  • However, because of the lack of tools to specifically inhibit the function of this integrin, little is known about its effects on cell behavior. (biologists.org)