• I've been using a couple of commercially available libraries (7mer to 12mer) to look for peptide sequences which bind cell surface receptors. (bio.net)
  • The library consists of approximately 1 x 10 9 electroporated (i.e., unique) sequences. (neb.com)
  • The library consists of approximately 10 9 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage. (neb.com)
  • Suited for all protease classes except carboxyproteases and those aminoproteases and dipeptidases requiring a free α-amine for cleavage, PICS simultaneously profiles the specificity of prime and nonprime positions and directly determines scissile peptide bonds of up to hundreds of cleavage site sequences in a single experiment. (springer.com)
  • As a general formula, if you made a completely random peptide library with n amino acids for each link in the chain with a length of x, the total number of possible sequences is n^x. (wikipedia.org)
  • A complication to this approach is that these peptide sequences need to first be identified, which can be challenging with viruses that undergo antigenic changes as a result of antigenic shift or drift, such as the influenza virus or HIV. (genscript.com)
  • These peptide sequences are used to predict the B- and T-cell epitopes to stimulate passive immunity. (genscript.com)
  • Recently, dynamic peptide libraries (DPLs) have been proposed to select self-assembling materials from dynamically exchanging mixtures of dipeptide inputs in the presence of a nonspecific protease enzyme, where peptide sequences are selected and amplified based on their self-assembling tendencies. (rsc.org)
  • These displaying phages can then be screened against other proteins, peptides or DNA sequences, in order to detect interaction between the displayed protein and those other molecules. (wikipedia.org)
  • Applications of phage display technology include determination of interaction partners of a protein (which would be used as the immobilised phage "bait" with a DNA library consisting of all coding sequences of a cell, tissue or organism) so that the function or the mechanism of the function of that protein may be determined. (wikipedia.org)
  • These application-specific peptide libraries can be used in applications such as vaccine development, T- and B-cell research, antibody development, biomarker discovery, and kinase and protease studies. (thermofisher.com)
  • Proteome-derived peptide libraries are generated by trypsin, GluC, or chymotrypsin digestion of biologically relevant proteomes, such as cytosolic lysates, to generate three separate libraries that each differ from the others in their C-terminal amino acid residues according to the protease specificity. (springer.com)
  • Primary amines of all peptides are then chemically protected so that after incubation with a test protease, the neo-N-termini of the prime-side cleavage products with exposed α-amines can be specifically biotinylated, enriched, and identified by liquid chromatography-tandem mass spectrometry. (springer.com)
  • Bicyclic peptide inhibitor reveals large contact interface with a protease target," ACS Chemical Biology , vol. 7, no. 5, pp. 817-821, 2012. (hindawi.com)
  • Peptide libraries, which are comprised of systematic combinations of antigenic peptide fragments, can accelerate the discovery and design of vaccines for these types of viral diseases. (genscript.com)
  • the mass spectra corresponding to the fragments of peptide precursor is recorded. (wikipedia.org)
  • Due to the complex nature of peptide fragmentation in a mass spectrometer, derivative fragmentation patterns fall short of reproducing experimental mass spectra, especially relative intensities among distinct fragments. (wikipedia.org)
  • Over years, we have integrated multiple peptide libraries with varied lengths and properties to satisfy all kind of applications. (labtube.tv)
  • The structures of the peptides exhibiting high affinity were characterised by Edman microsequencing. (diva-portal.org)
  • This ultrasound-based method enables single-step selection of a high-affinity peptide. (wikipedia.org)
  • Achieved by substituting selected amino acid residues by all other natural amino acids one at a time, it has the capability to locate potential more favorable residue(s) at specified position(s) for enhanced peptide activity. (genscript.com)
  • Optional peptide modifications are available, including residue phosphorylation and acetylation, and different formulations are possible that enable study of cellular signaling events. (thermofisher.com)
  • The displayed peptide is expressed at the N-terminus of pIII, i.e., the first residue of the mature protein is the first randomized position. (neb.com)
  • In this way, you could create a library of 20 different polypeptides with only one amino acid residue at random and the rest being the same. (wikipedia.org)
  • Here, we present a method comprising binding studies of serum antibody pools to synthetic random peptide libraries, and data analysis of the resulting binding patterns. (nih.gov)
  • Authoritative and practical, Peptide Libraries: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies. (springer.com)
  • If none of the premade libraries meets your requirement, you are welcome to contact us, and we will be more than happy to create a custom library for you. (creative-biolabs.com)
  • Empowered by extensive experience and well-established phage display technique platform, Creative Biolabs now brings out a comprehensive list of premade peptide libraries for various research programs. (labtube.tv)
  • In this approach, a protein sequence database is used to calculate all putative peptide candidates in the given setting (proteolytic enzymes, miscleavages, post-translational modifications). (wikipedia.org)
  • It's constructed by substituting selected positions on the original peptide randomly and simultaneously with all other natural amino acids in a shot gun approach with a purpose to elucidate potential alternatives for enhanced peptide activity. (genscript.com)
  • In order to elucidate the physiological function of the cell surface glycosoaminoglycan hyaluronan (HA), Mummert and co-workers (32) panned the Ph.D.-12 library against purified HA. (neb.com)
  • Chapter focus on methods and techniques for synthesis, genetic expression, hybrid synthesis-expression, examples of modern utility of these libraries, de novo discovery of reactions, hybrid organic-inorganic materials and, emerging tools for the analysis of these libraries by method of genetic selection and next-generation sequencing. (springer.com)
  • These random peptide libraries with such great quality and diversity can definitely make excellent tools for peptide agent discovery. (labtube.tv)
  • Alanine, the smallest chiral natural amino acid is used to substitute non-alanine residues at each position of the original peptide. (genscript.com)
  • Say you wanted a peptide chain 10 residues in length to use in native chemical ligation with a larger recombinantly expressed protein. (wikipedia.org)
  • If peptides do not pass these criteria, the customer will be informed and one resynthesis will be offered free of charge. (thermofisher.com)
  • Due to the design of the library and the sophisticated analysis, the method is able to classify and predict the different mice strains and the infection with very high accuracy and with a very small number of peptides, illustrating the potential of random library screenings in determining molecular markers for diagnosis. (nih.gov)
  • Sequence database searching also demands vast search space, which still could not cover all possibilities of peptide dynamics, exhibiting limited efficiency post-translational modifications). (wikipedia.org)
  • The predominant selected peptide (Pep1) was determined to bind both free HA and HA expressed on cell surfaces, as well as to block HA binding to cell surfaces expressing the HA receptor, CD44. (neb.com)
  • Subsequently, one or more chromatographic separations are applied to resolve resulting peptides, which are then ionized and analyzed in a mass spectrometer. (wikipedia.org)
  • Natural product-like macrocyclic N-methyl-peptide inhibitors against a ubiquitin ligase uncovered from a ribosome-expressed de novo library," Chemistry & Biology , vol. 18, no. 12, pp. 1562-1570, 2011. (hindawi.com)
  • A selected peptide sequence was found to inhibit proliferation of primary human vein endothelial cells, to have antiangiogenic effects in chick embryos, and finally to reduce breast tumor growth and metastasis by 70% in mice. (neb.com)
  • Bicyclic peptides with optimized ring size inhibit human plasma kallikrein and its orthologues while sparing paralogous proteases," ChemMedChem , vol. 7, no. 7, pp. 1173-1176, 2012. (hindawi.com)
  • Epitope mapping using randomly generated peptide libraries. (nih.gov)
  • As validated by QC DNA sequencing, over 97% of randomly picked clones from these two libraries contained dsDNA inserts, with no stop codon, single nucleotide deletion or insertion or non-sense frame-shift mutation detected. (labtube.tv)