• TCR sequencing was performed with the ImmunoSEQ assay in 49 and 39 pretreatment and post-treatment patient peripheral blood mononuclear cell (PBMC) samples. (nih.gov)
  • Since the KCs are Class II histocompatibility antigen (HLA-DR) positive in these diseases (indicating local production of gamma interferon, IFN-γ, by activated T-cells), we sought to determine whether IFN-γ treatment of KCs would influence the ability of allogeneic peripheral blood mononuclear leukocytes (PBMLs) to adhere to cultured KCs in vitro. (elsevierpure.com)
  • Nickoloff, BJ , Lewinsohn, DM & Butcher, EC 1987, ' Enhanced binding of peripheral blood mononuclear leukocytes to γ-interferon-treated cultured keratinocytes ', American Journal of Dermatopathology , vol. 9, no. 5, pp. 413-418. (elsevierpure.com)
  • Third, peripheral blood mononuclear cells are used to explore heterogeneous immune populations. (nih.gov)
  • In mixed peripheral blood mononuclear cells (PBMCs) from the same individuals, we show to what extent mitochondrial measures are confounded by both cell type distributions and contaminating platelets. (nih.gov)
  • The objective of this study is to explore the long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) expression profiles of monocyte-derived dendritic cells (DCs) obtained from peripheral blood mononuclear cells (PBMCs). (frontiersin.org)
  • The peripheral blood mononuclear cells (PBMCs) have a round nucleus ( Delves, 2016 ). (frontiersin.org)
  • In mice, we looked at the transcriptomic changes in muscle at the injection site, the lymph node that drained the muscle, and the peripheral blood mononuclear cells (PBMCs)isolated from the circulating blood from 4 hr after injection and over the next week. (elifesciences.org)
  • C. parapsilosis -stimulated human peripheral blood mononuclear cells (PBMCs) produced similar quantities of tumor necrosis factor α and interleukin 6 and slightly lower amounts of interleukin 1β, compared with C. albicans-stimulated cells. (bath.ac.uk)
  • We performed chromatin immunoprecipitation sequencing (ChIP-seq) using a histone 3 lysine 9 acetylation (H3K9ac) mark in peripheral blood mononuclear cells from patients with atherosclerosis with T2D (n = 8) or without T2D (ND, n = 10). (lu.se)
  • Although phagocytosis and killing by mononuclear leukocytes was less efficient than that by polymorphonuclear leukocytes, only mononuclear leukocytes were able to degrade E. coli PC2166 DNA. (jci.org)
  • Although we observed no DNA degradation during phagocytosis by polymorphonuclear leukocytes, lysates of both polymorphonuclear and mononuclear leukocytes contained acid-DNase activity with a pH optimum of 4.9. (jci.org)
  • However, the DNase activity of mononuclear leukocytes was 20 times higher than that of polymorphonuclear leukocytes. (jci.org)
  • Total cell number, mononuclear phagocytes, polymorphonuclear leukocytes, lymphocytes, and LDH levels were significantly increased in ASB and CNT-exposed mice. (cdc.gov)
  • Lung examination findings reveal an interstitial pneumonitis made up of edema fluid, mononuclear cells, and lymphocytes with polymorphonuclear leukocytes. (medscape.com)
  • The initial tissue reaction to infection is a focal, intensely suppurative necrosis consisting largely of accumulations of polymorphonuclear leukocytes, followed by invasion of macrophages, epithelioid cells, and lymphocytes. (cdc.gov)
  • DS patients also present a T cell immunodeficiency causing functional defects of polymorphonuclear leukocytes, reduced chemotaxis, diminished phagocytic ability, defective oxidative response and abnormal bactericidal activity 3 . (bvsalud.org)
  • Second, while extending studies in animal models such as these to humans takes substantially more work, these new data point to a potentially novel drug target for MS and possibly other autoimmune or central nervous system diseases," said John Wherry, Ph.D., Deputy Editor of the Journal of Leukocyte Biology. (disabled-world.com)
  • Slight cuffing with polynuclear cells, mononuclear leukocytes. (cdc.gov)
  • reports exist of elevated counts of neutrophils or mononuclear cells. (medscape.com)
  • Cancer cells and mononuclear cells were isolated, cultured overnight, and analyzed for viability using flow cytometry (FC). (aiche.org)
  • Exposure of functionalized ES/TRAIL leukocytes into cancer cell-spiked blood successfully targeted and killed cancer cells under shear flow, compared to controls (Fig. 1B). (aiche.org)
  • Cancer cells lodged within the mouse lung were apoptotic after treatment with ES/TRAIL leukocytes, as determined by multiphoton microscopy. (aiche.org)
  • The migration of inflammatory leukocytes, including dendritic cells, neutrophils, and macrophages, to the peritoneal mesentery has been shown to be promoted in LAT −/− (lack mature T cells) mice but inhibited in μ MT (lack mature B cells) mice during the pristane-induced immune response [ 10 ]. (hindawi.com)
  • No. 557983), and mononuclear cells were identified by scatter profile. (bdbiosciences.com)
  • Nonviable cells were eliminated from analysis by staining with propidium iodide, and all viable leukocytes are displayed. (bdbiosciences.com)
  • The sporozoites invade mononuclear cells and disseminate hematogenously or via the lymph to target organs. (vin.com)
  • The samples were analyzed in duplicate by the Yumizen H2500 to determine the number of leukocytes and erythrocytes, the partial formula (% of mononuclear cells (%MN) and % of polymorphonuclear cells (%PN)) and to detect abnormal cells. (horiba.com)
  • Scholars@Duke publication: Human mononuclear leukocyte chemotaxis: a quantitative assay for humoral and cellular chemotactic factors. (duke.edu)
  • Here, we describe a novel approach to functionalize human and murine leukocytes with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on their surface, along with E-selectin (ES), to capture and induce cancer cell apoptosis both in vitro in human blood and in vivo in mouse circulation. (aiche.org)
  • Note: Tonsil Specimens- is recommended to screen several hyper-reactive tonsils to find those with highest expression of PD-L1 in crypt epithelium, macrophages homing the germinal centers and interfollicular mononuclear leukocytes. (abcam.com)
  • Many tumor specimens have some inflammatory macrophages and mononuclear leukocytes. (abcam.com)
  • Neutrophils, haemorrage, necrosis, oedema and activated vascular endothelium represent components of an active inflammatory process, at the same time chronicity is observed as fibroplasia, mononuclear leukocytes and neovascularisation are present in the tissue. (helsinki.fi)
  • It is believed that adhesive interactions between selectins on the blood vessel wall and selectin ligands on the CTC surface facilitate metastatic progression, in a manner similar to the leukocyte adhesion cascade essential in the inflammatory response (3). (aiche.org)
  • We and others have discovered factors that control the recruitment of inflammatory leukocytes to the peritoneal mesentery in response to pristane [ 9 , 10 ]. (hindawi.com)
  • The polymorphonuclear leukocyte-neutrophil is a key cell type and an essential part of the host's inflammatory response. (bvsalud.org)
  • These mitochondrial phenotyping data build upon established immunometabolic differences among leukocyte subpopulations, and provide foundational quantitative knowledge to develop interpretable blood-based assays of mitochondrial health. (nih.gov)
  • To further analyze the capacity of mononuclear leukocytes to degrade E. coli DNA, chromosomal and plasmid DNA was isolated from ingested bacteria and subjected to agarose gel-electrophoresis. (jci.org)
  • Merogony occurs in the dog's parenchymal tissues and is followed by gamontogony in leukocytes. (vin.com)
  • Though this method can provide insight into skin-infiltrating leukocyte biology, it can also be applied to the investigation of other resident cell populations. (jove.com)
  • Histamine could be an important molecule to developing new treatments for multiple sclerosis (MS). More than allergies: Histamine may be a possible drug target for multiple sclerosis - New research published in the Journal of Leukocyte Biology suggests that histamine plays an important role as an immune modulator, which could be a significant finding for multiple sclerosis research. (disabled-world.com)
  • More than allergies: Histamine may be a possible drug target for multiple sclerosis - New research published in the Journal of Leukocyte Biology suggests that histamine plays an important role as an immune modulator, which could be a significant finding for multiple sclerosis research. (disabled-world.com)
  • New research published in the Journal of Leukocyte Biology (www.jleukbio.org) shows that histamine could be an important molecule to developing new treatments for multiple sclerosis (MS). In the study, the scientists analyzed the role of histamine in an animal model of multiple sclerosis and found that histamine plays a critical role in preventing MS or lessening its effects. (disabled-world.com)
  • The Journal of Leukocyte Biology is published by the Society for Leukocyte Biology. (disabled-world.com)
  • Citations to Effect of human polymorphonuclear and mononuclear leukocytes on chromosomal and plasmid DNA of Escherichia coli. (jci.org)
  • Effect of human polymorphonuclear and mononuclear leukocytes on chromosomal and plasmid DNA of Escherichia coli. (jci.org)
  • To determine leukocyte functionalization with ES/TRAIL, fluorescent (FL) liposomes were exposed to human blood under shear flow, and leukocytes were isolated and analyzed for ES/TRAIL attachment using FC and confocal microscopy. (aiche.org)
  • Upon exposure to shear flow, ES/TRAIL liposomes in human blood were functionalized to the surface of leukocytes, as confirmed by confocal microscopy (Fig. 1A) and FC. (aiche.org)
  • We have shown that leukocytes functionalized with ES/TRAIL can capture and induce cancer cell apoptosis in vitro in human blood and in vivo in mouse experiments. (aiche.org)
  • Interferon-Like Virus-Inhibitor Induced in Human Leukocytes by Phytohemagglutinin. (wikidata.org)
  • The transcription factor 7-like 2 (TCF7L2) rs7903146, together with several human leukocyte antigen (HLA) variants, were among the domains with the most dramatic changes of H3K9ac enrichments. (lu.se)
  • In our body's immune system , the Lymphocyte (Lymphoid Cell) is a type of white blood cell (wbc) (aka leukocytes) , derived from a common progenitor cell originating in the bone marrow, then formed in the lymphoid tissue, with nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. (wellnessadvantage.com)
  • We also detected changes in energy utilization and immune responses in leukocytes from older cows. (biomedcentral.com)
  • No difference was observed between DNase activity from polymorphonuclear and mononuclear leukocytes from a chronic granulomatous disease patient with DNase activity from control polymorphonuclear and mononuclear leukocytes. (jci.org)
  • Phagocytosis and killing by polymorphonuclear and mononuclear leukocytes are important host resistance factors against invading microorganisms. (jci.org)
  • This study analysed the transcriptome derived from RNA-seq data of leukocytes obtained from Holstein cows in early lactation with respect to lactation number. (biomedcentral.com)
  • In addition, data from this study allowed the leukocyte count and the scattergram thresholds used for the differential to be optimized. (horiba.com)
  • 74 out of 98 samples were used to compare leukocyte and erythrocyte count. (horiba.com)
  • High parasitemia rates are sometimes accompanied by extreme neutrophilia reaching as high as 150,000 leukocytes/µl blood. (vin.com)
  • Dermatophatologists observe mononuclear leukocytes in close apposition to keratinocytes (KCs) in graft versus host disease and in other lymphocyte-mediated skin diseases, such as lichen planus, erythema multiforme, and lupus erythematosus. (elsevierpure.com)
  • cutánea maligna de células T. Luego se realizó el frotis de médula ósea, en el que se identificaron células «cerebriformes» study conception, manuscript que confirmaron el diagnóstico de síndrome de Sézary. (bvsalud.org)
  • The correlation coefficient between the 2 analyzers was 0.991 for leukocyte (figure 2) and 0.955 between Yumizen H2500 and the conventional chamber counting method (figure 3). (horiba.com)