Leukocytes human leukocyte. Medical search. Frequent questions

To assess the role of shark cartilage as an immune modulator, acid, salt-soluble, and phosphate-buffered saline extracts were prepared from three different commercial sources (SL, TL, FDC) of cartilage and used to stimulate human leukocytes in vitro. (fiu.edu)
Here, we describe a novel approach to functionalize human and murine leukocytes with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on their surface, along with E-selectin (ES), to capture and induce cancer cell apoptosis both in vitro in human blood and in vivo in mouse circulation. (aiche.org)
We have shown that leukocytes functionalized with ES/TRAIL can capture and induce cancer cell apoptosis in vitro in human blood and in vivo in mouse experiments. (aiche.org)
So far, most of the in vivo and in vitro approaches to study leukocyte migration across the tissue parenchyma have addressed the role of each single tissue barrier that immune cells encounter in their journey to the inflammatory focus. (hindawi.com)
The potential of recombinant ALP to block the interactions of leukocytes with the endothelial lining was concomitantly investigated in vitro and in vivo . (biomedcentral.com)
An IL-1β-activated endothelial layer formed by a murine glomerular cell line (glEND.2) was used to assay the interaction with human leukocytes in vitro . (biomedcentral.com)

We investigated the contractile effects of both activated and unactivated polymorphonuclear leukocytes (PMNs) on human vascular tissue to characterize the influence of human PMNs on vascular tone. (aspetjournals.org)
Microfilaments and microtubules in calcium ionophore-induced secretion of lysosomal enzymes from human polymorphonuclear leukocytes. (rupress.org)
The effect of chemotherapeutic agents - cytosine arabinoside, methotrexate, procarbazine, vinblastine and vincristine - generally applied in the treatment of human cancer were tested on the motility of polymorphonuclear leukocytes. (karger.com)

In the present study, we demonstrate that following exposure to plasma from diabetic patients, the human promonocytic cell line U937 exhibits a significant elevation in core 2 GlcNAc-T activity and increased adherence to cultured retinal capillary endothelial cells. (diabetesjournals.org)
On the one hand, similar to endothelial cells, parenchymal epithelia must guide leukocytes to traverse them in order to reach a localized inflammatory focus, for example, in the lung or intestinal mucosa. (hindawi.com)
These 2 members mediate leukocyte adhesions to endothelial cells but they also serve as receptors for iC3b (inactivated C3b). (medscape.com)
This disease is a defect in fucose metabolism (lack of fucosylation of the carbohydrate selectin ligands) that results in failure to express the ligand for E and P selectin, sialyl Lewis-X (CD15s) expressed on leukocytes and endothelial cells. (medscape.com)

The purpose of this study was to determine the effects of increased training via cross-training (run + cycle) and run training on circulating leukocyte subsets. (nih.gov)
The elements were evaluated by the total number of peripheral leukocyte, granulocyte and lymphocyte ratio and lymphocyte subsets. (scirp.org)
The number of leukocyte subsets, granulocyte and lymphocyte were also regulated by dose dependent manner. (scirp.org)

The events that follow leukocyte extravasation are perhaps less well characterized, although significant advances have been made with the advent of high-resolution intravital microscopy and the development of more sophisticated culture systems to investigate leukocyte migration and interactions in three dimensions. (hindawi.com)
We believe that successfully combining our current knowledge about leukocyte extravasation, three-dimensional migration through the stroma and the sequential interactions with parenchymal cell barriers, which include adopting unified experimental models, will help shed light on the entire migratory route of each immune cell type and on the specificity of the innate inflammatory responses in each type of tissue. (hindawi.com)

Of the 8 plasma membrane receptors for complement, only deficiencies of CR3 and CR4 due to CD18 deficiency have been described, known as leukocyte adhesion deficiency (LAD) type 1 . (medscape.com)
Leukocyte adhesion deficiency (LAD) is a rare primary immunodeficiency. (medscape.com)
Thus the infections in patients with leukocyte adhesion deficiency act similarly as those observed in patients with neutropenia. (medscape.com)
Labial ulceration from which Escherichia coli was cultured in an 8-month-old girl with leukocyte adhesion deficiency type 1 (LAD I). Note the thin bluish scar at the superior aspect of the labia from an earlier cellulitis. (medscape.com)
This 3-year-old girl had leukocyte adhesion deficiency type I (LAD I) with complete absence of CD18 expression. (medscape.com)
This 10-month-old patient with severe leukocyte adhesion deficiency type I (LAD I) developed a cervical adenitis caused by Klebsiella pneumoniae. (medscape.com)
Leukocyte adhesion deficiency type I (LAD I) is a failure to express CD18, which composes the common ß 2 subunit of LFA1 family (ß2 integrins). (medscape.com)
In milder forms of leukocyte adhesion deficiency I (1-30% expression of CD8), patients may survive to adulthood. (medscape.com)
Patients with leukocyte adhesion deficiency II manifest the Bombay phenotype (ie, negative for O and H blood group antigens with potential production of anti-H antibody). (medscape.com)
However, IgM and IgG serum levels are within the reference range in patients with leukocyte adhesion deficiency II. (medscape.com)
Leukocyte adhesion deficiency II may be classified as one of the congenital disorders of glycosylation (CDG), a rapidly expanding group of metabolic syndromes with a wide symptomatology and severity. (medscape.com)
Currently, 18 subtypes have been reported: 12 are type I (dysfunctional lipid-linked oligosaccharide precursor synthesis), and 6 are type II (dysfunctional trimming/processing of the protein-bound oligosaccharide), including leukocyte adhesion deficiency II (CDG-IIc). (medscape.com)
Variants of leukocyte adhesion deficiency have also been reported, including fully expressed but nonfunctional CD18 and an E selectin that is expressed but rapidly cleaved from the cell surface (only present in soluble form). (medscape.com)
Another reported type of leukocyte adhesion deficiency involves dysfunction in platelet aggregation in addition to a defect in leukocyte adhesion. (medscape.com)
Thus, patients with this type of leukocyte adhesion deficiency manifest both severe bacterial infections and bleeding disorder. (medscape.com)
This leukocyte adhesion deficiency variant is associated with defective expression of the Rap-1 activator CalDAG-GEFI. (medscape.com)

Oncolytic vesicular stomatitis virus (VSV) can be delivered intravenously to target primary and metastatic lesions, but the interaction between human peripheral blood leukocytes (PBLs) and VSV remains poorly understood. (karger.com)
Human peripheral blood leukocytes (PMN) are induced to release lysosomal enzymes by the calcium ionophore A23187 in the presence but not the absence of extracellular Ca++. (rupress.org)
Twelve subpanels of monoclonal antibodies (MAb) included within the 6th International Workshop on Human Leukocyte Differentiation Antigens (6th HLDA) were assayed for reactivity with bovine peripheral blood leukocytes. (ox.ac.uk)

In humans, MHC proteins are encoded by the Human Leukocyte Antigen (HLA), a group of more than 200 genes located closely together on the short arm of chromosome 6. (antibodies-online.com)
Classical class I and class II Human Leukocyte Antigen (HLA) are leading candidates for infectious disease susceptibility. (antibodies-online.com)
for example the MHC Class II antibody based on clone IVA12 recognizes the shared epitopes of human leucocyte antigen (HLA) class II molecules HLA-DP, HLA-DQ and HLA-DR heterodimeric cell surface glycoproteins comprised of an α (heavy) chain and a β (light) chain. (antibodies-online.com)
Human Leukocyte Antigen Susceptibility Map for Severe Acute Respiratory Syndrome Coronavirus 2. (antibodies-online.com)
These were investigated further by two-colour immunofluorescence to compare the cellular expression of the antigen on cattle cells with that reported for the different CD antigens in humans. (ox.ac.uk)
Four of the MAb that belonged to CD23, CD39, CD47, and CDw149 stained bovine cells in a manner that indicated an almost identical cellular distribution of the antigen to that reported in humans. (ox.ac.uk)

On the basis of its potential to block anti-collagen type II (CII) antibody-induced arthritis (CAIA) and to suppress the conformational activation of β 2 -integrins in leukocytes, the present study was undertaken to investigate its interference with leukocyte adherence to cytokine-activated endothelium. (biomedcentral.com)

Leukocyte recruitment into the inflamed parenchyma requires successive interactions with cellular and stromal barriers that establish mechanical, chemotactic and haptotactic gradients to guide immune cells towards the inflammatory focus. (hindawi.com)
[ 1 , 2 ] The clinical picture is characterized by marked leukocytosis and localized bacterial infections that are difficult to detect until they have progressed to an extensive level secondary to lack of leukocyte recruitment at the site of infection. (medscape.com)

Previous studies have identified many immune pathways which are consistently altered in humans and model organisms as they age. (biomedcentral.com)
Genes and pathways associated with lactation number in cows were identified for the first time to date, and we found that many were comparable to those known to be associated with ageing in humans and model organisms. (biomedcentral.com)

Here we review evidence showing that apicobasal polarity regulates the inflammatory response: various polarized epithelia asymmetrically secrete chemotactic mediators and polarize adhesion receptors that dictate the route of leukocyte migration within the parenchyma. (hindawi.com)
The first stage of this immune steeplechase, the leukocyte transendothelial migration, is a multi-step cascade of interactions that have been extensively studied in recent years in different vascular beds and experimental models, and some comprehensive reviews on this topic can be found in this special issue [ 1 - 5 ]. (hindawi.com)
Endothelial and epithelial barriers determine the different stages of leukocyte migration in its journey towards the inflammatory focus in complex tissues. (hindawi.com)
The parenchymal three-dimensional organization contributes to establish an haptotactic and chemotactic gradient (1) Leukocyte adhesion and transendothelial migration or diapedesis. (hindawi.com)

PMNs were added either unactivated or after f-met-leu-phe (fMLP) activation (10 −8 M), into tissue chambers containing human umbilical vein segments under either control or cytokine-treated conditions. (aspetjournals.org)
Particular attention has been paid to elucidating how leukocytes can migrate through the stroma, the way these cells remodel their morphology and sense cues that guide them towards dysfunctional tissue areas. (hindawi.com)

It is believed that adhesive interactions between selectins on the blood vessel wall and selectin ligands on the CTC surface facilitate metastatic progression, in a manner similar to the leukocyte adhesion cascade essential in the inflammatory response (3). (aiche.org)
We also discuss recent findings showing that the loss of apicobasal polarity increases leukocyte adhesion to epithelial cells and the consequences that this could have for the inflammatory response towards damaged, infected or transformed epithelial cells. (hindawi.com)
On the other hand, these barriers contain damaged or infected cells that are part of the inflammatory focus and the endpoint of the leukocyte migratory journey, so some sort of footprint, which is not completely understood, must exist in these cells to promote a preferential adhesion with infiltrated leukocytes. (hindawi.com)

Though this method can provide insight into skin-infiltrating leukocyte biology, it can also be applied to the investigation of other resident cell populations. (jove.com)

Kupffer cells, hepatic stellate cells and sinusoidal endothelium) and infiltrating leukocytes (e.g., monocytes, monocyte-derived macrophages, neutrophils and lymphocytes). (mdpi.com)
Leukocyte rolling and firm adhesion to the synovial endothelium in an early response to the anti-CII antibody transfer were significantly decreased in ALP-pretreated mice. (biomedcentral.com)
Preventive administration of ALP as a single dose of 100 μg per mouse led to a significant suppression of leukocyte rolling on, and of firm adhesion to, the synovial venular endothelium. (biomedcentral.com)

Duplicate leukocyte cultures were set up, each containing 50 $\mu$l of endotoxin-free extract, 200 $\mu$l of cell suspension (2.4-2.5 $\times$ 10$\sp5$ cells) and 100 $\mu$l of medium and incubated at 37$\sp\circ$C. Cultures stimulated with LPS (5 $\mu$g/ml) or medium served as the positive and negative controls, respectively. (fiu.edu)

To determine leukocyte functionalization with ES/TRAIL, fluorescent (FL) liposomes were exposed to human blood under shear flow, and leukocytes were isolated and analyzed for ES/TRAIL attachment using FC and confocal microscopy. (aiche.org)

We also detected changes in energy utilization and immune responses in leukocytes from older cows. (biomedcentral.com)

Upon exposure to shear flow, ES/TRAIL liposomes in human blood were functionalized to the surface of leukocytes, as confirmed by confocal microscopy (Fig. 1A) and FC. (aiche.org)
Exposure of functionalized ES/TRAIL leukocytes into cancer cell-spiked blood successfully targeted and killed cancer cells under shear flow, compared to controls (Fig. 1B). (aiche.org)

Both unactivated and activated PMNs induced a cell number-dependent (1.5 × 10 5 to 2 × 10 6 cells/ml) vasoconstriction in human umbilical vein segments. (aspetjournals.org)
A carrier state of mumps virus in human conjunctiva cells. (wikidata.org)
205 or PC-3 cancer cells suspended in buffer or spiked in peripheral human blood were subjected to shear flow in a cone-and-plate viscometer. (aiche.org)
Cancer cells lodged within the mouse lung were apoptotic after treatment with ES/TRAIL leukocytes, as determined by multiphoton microscopy. (aiche.org)
Cross-reactivity with bovine cells of monoclonal antibodies submitted to the 6th International Workshop on Human Leukocyte Differentiation Antigens. (ox.ac.uk)
Absolute other hand, another study reported that the neutrophil count was calculated as fol- phagocytic activity of neutrophils was simi- lows: absolute value (neutrophils/mm3) = lar in women in the 1st and 2nd trimesters total leukocyte count (cells/mm3) × relative of pregnancy and non-pregnant women, but value (%) obtained from the differential significantly decreased during the 3rd tri- count [10]. (who.int)
The white blood cells (leukocytes) are further divided into phagocytes or myeloid (neutrophils, eosinophils, basophils, monocytes) and immunocytes or lymphoid (lymphocytes). (medscape.com)

Total leukocyte count was estimated manu- nant women of reproductive age [1]. (who.int)
The mean total leukocyte count increased blood. (who.int)

Differentially expressed candidate genes for ageing previously identified in the human blood transcriptome up-regulated in PP cows were mainly associated with T-cell function ( CCR7 , CD27 , IL7R , CAMK4 , CD28 ), mitochondrial ribosomal proteins ( MRPS27 , MRPS9 , MRPS31 ), and DNA replication and repair ( WRN ). (biomedcentral.com)

This study analysed the transcriptome derived from RNA-seq data of leukocytes obtained from Holstein cows in early lactation with respect to lactation number. (biomedcentral.com)
Approximately 0.05% of an applied dose (0.0026 mg/cm2) was determined in one human study (Franz 1984), and another estimated a dermal absorption rate of approximately 0.4 mg/cm2/hour under conditions of complete saturation (35-43 cm2 of skin was exposed to approximately 0.06 g/cm2 of liquid benzene for 1.25-2 hours) (Hanke et al. (cdc.gov)

Purpose: Leukocyte telomere length has gained attention as a marker of oxidative damage and age-related diseases, including cancer. (aacrjournals.org)
Ticks (Acari: Ixodidae and Argasidae) transmit multiple and diverse pathogens (including bacteria, protozoa, and viruses), which cause a wide range of human and animal diseases, including rickettsial diseases, caused by bacteria in the order Rickettsiales. (cdc.gov)
Awareness, diagnosis, and control of tickborne rickettsial diseases are most effectively addressed by considering the intersecting components of human, animal, and environmental health that collectively form the foundation of One Health ( 1 ), an approach that integrates expertise from multiple disciplines and facilitates understanding of these complex zoonoses. (cdc.gov)
Tickborne rickettsial diseases in humans often share similar clinical features yet are epidemiologically and etiologically distinct. (cdc.gov)

which leukocyte type would likely be elevated in a patient who has strep throat? (bartleby.com)

I. Immunological Unresponsiveness Induced in Embryonic Mice by Maternofetal Transfer of Human-Globulin. (scirp.org)
A statistical analysis of intravital microscopic images recorded from the knee joints of untreated control mice during an early time span of 24 hours after the transfer of anti-CII mAbs [ 10 ] revealed an antibody-induced increase in leukocyte adhesion to the vessel walls. (biomedcentral.com)

Floor heating system regulated total number of peripheral leukocyte. (scirp.org)

In this report, we intended to clarify the effect of floor hating system for accessing a peripheral leukocyte regulation in winter season by evidence-based manner more than VAS. (scirp.org)

We recently reported that raised activity of the glycosylating enzyme core 2 β 1,6- N -acetylglucosaminyltransferase (GlcNAc-T) through protein kinase C (PKC)β2-dependent phosphorylation plays a fundamental role in increased leukocyte-endothelial cell adhesion and capillary occlusion in retinopathy. (diabetesjournals.org)
Vertebrate animals play an integral role in the life cycle of tick species, whereas humans are incidental hosts. (cdc.gov)

The exact pathogenic mechanism by which capillary nonperfusion, as demonstrated by fluorescein angiography, occurs is still unclear, but recent experimental animal studies ( 3 - 5 ) demonstrate that increased leukocyte entrapment in retinal capillaries of diabetic rats is an early event associated with areas of capillary nonperfusion and the development of endothelial cell damage. (diabetesjournals.org)

A large body of evidence now implicates increased leukocyte-endothelial cell adhesion as a key early event in the development of diabetic retinopathy. (diabetesjournals.org)

This protocol describes enzymatic digestion of mouse skin in nutrient-rich medium followed by gradient separation to isolate leukocytes. (jove.com)

Quantitative oral absorption data are not available for benzene in humans. (cdc.gov)

In order to be human-readable, please install an RSS reader. (mdpi.com)

These results suggest that human PMNs of varying activation states have the capacity to modulate vascular smooth muscle tone via distinct mechanisms. (aspetjournals.org)

Anatomy29

Organisms11

Diseases17

Chemicals and Drugs118

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Psychiatry and Psychology2

Phenomena and Processes52

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Information Science4

Named Groups2

Health Care4

Vitro6

Polymorphonuclear leukocytes3

Endothelial cells4

Subsets3

Extravasation2

Adhesion deficiency16

Peripheral blood leukocytes3

Antigen6

Adherence1

Recruitment2

Pathways2

Migration4

Tissue2

Inflammatory3

Biology1

Endothelium3

Cultures1

Attachment1

Immune1

Exposure2

Cells7

Count2

Blood1

Study2

Diseases4

Type1

Mice2

Total1

Effect1

Role2

Studies1

Early1

Mouse1

Data1

Order1

Results1