Leukocyte to p selectin. Medical search. Frequent questions

This Mouse Endothelial Leukocyte Adhesion Molecule 1 (E-Selectin) ELISA Kit from Innovative Research is intended for quantitative detection of mouse soluble E-Selectin in cell culture supernates, serum and plasma (heparin, citrate). (innov-research.com)
Expression system for standard: E-selectin, also called endothelial-leukocyte adhesion molecule-1(ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. (innov-research.com)
A structurally and functionally related group of genes, lymph node homing receptor(LHR), granule membrane protein 140(GMP-140), and endothelial leukocyte adhesion molecule 1(ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. (innov-research.com)

L-selectin plays an important role in both the innate and adaptive immune responses by facilitating leukocyte-endothelial cell adhesion events. (wikipedia.org)

Thrombin-activated human peripheral blood platelets were stained with CD62P (P-Selectin) (clone AK4) Brilliant Violet 650™ (filled histogram) or mouse IgG1, κ Brilliant Violet 650™ isotype control (open histogram). (biolegend.com)
This study indicates that alfrutamide could suppress P-selectin expression on platelets and platelet-leukocyte interactions by inhibiting COX enzymes, meanwhile caffedymine could do the same suppression by inhibiting COX enzymes as well as increasing cAMP. (usda.gov)
At the concentration of 0.1 microM, they were also able to inhibit P-selectin expression on the platelets by 28 % (P (usda.gov)
In particular, we are interested in applying quantitative experimental and computational techniques to discover new mechanisms that control the cell adhesion properties of blood leukocytes and platelets during human inflammatory and thrombotic disorders. (buffalo.edu)
E-selectin is expressed on the surface of endothelial cells and mediates the interaction of leukocytes and platelets with endothelial cells during an inflammatory response. (prospecbio.com)
As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin. (wikidoc.org)
The first step in this interaction process is carried out by PSGL-1 interacting with P-selectin and/or E-selectin on endothelial cells and adherent platelets. (wikidoc.org)

An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. (innov-research.com)
In addition to its function in the immune response, L-selectin is expressed on embryonic cells and facilitates the attachment of the blastocyst to the endometrial endothelium during human embryo implantation. (wikipedia.org)
While L-selectin ligands on the apical side of the endothelium have long been characterized as receptors for binding and rolling, glycans enriched on the basolateral side and in the basement membrane likely control quite different signals. (wikipedia.org)
On the basis of its potential to block anti-collagen type II (CII) antibody-induced arthritis (CAIA) and to suppress the conformational activation of β 2 -integrins in leukocytes, the present study was undertaken to investigate its interference with leukocyte adherence to cytokine-activated endothelium. (biomedcentral.com)
Leukocyte rolling and firm adhesion to the synovial endothelium in an early response to the anti-CII antibody transfer were significantly decreased in ALP-pretreated mice. (biomedcentral.com)
Preventive administration of ALP as a single dose of 100 μg per mouse led to a significant suppression of leukocyte rolling on, and of firm adhesion to, the synovial venular endothelium. (biomedcentral.com)
TNF-a then induces the expression of leukocyte adhesion molecules upon proximal septal capillary endothelium resulting in the recruitment of inflammatory leukocytes to the site. (cdc.gov)

Of the 8 plasma membrane receptors for complement, only deficiencies of CR3 and CR4 due to CD18 deficiency have been described, known as leukocyte adhesion deficiency (LAD) type 1 . (medscape.com)
These 2 members mediate leukocyte adhesions to endothelial cells but they also serve as receptors for iC3b (inactivated C3b). (medscape.com)
Beta 2-adrenoceptor antagonists (butoxamine and ICI 118551) partially blocked the P-selectin expression inhibition by caffedymine, suggesting that beta-2 receptors are likely involved in the inhibition, but no effect on the inhibition by alfrutamide. (usda.gov)
In addition, tumour cells have co-opted some of the signalling molecules of the innate immune system, such as selectins, chemokines and their receptors for invasion, migration and metastasis. (weeksmd.com)
Corresponding receptors on leukocytes probably enable a selective recruitment of different leukocyte populations in inflammatory corneal diseases. (nih.gov)
The selectin adhesion molecules and chemoattractant receptors synergistically regulate leukocyte migration into lymphoid tissues and sites of inflammation, but little is known about how these families of receptors modulate each other's function. (upenn.edu)
Interactions between L-selectin and chemoattractant receptors were therefore examined using transfected rat basophilic leukemia cell lines (RBL-2H3) that expressed human L-selectin along with human leukocyte chemoattractant receptors. (upenn.edu)

CD62P is a 140 kD type I transmembrane glycoprotein also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. (biolegend.com)
Another reported type of leukocyte adhesion deficiency involves dysfunction in platelet aggregation in addition to a defect in leukocyte adhesion. (medscape.com)
Consequently, they could suppress platelet-leukocyte interactions in blood samples by 24-32 % (P (usda.gov)
These data suggest that the amides are able to suppress P-selectin expression and platelet-leukocyte interactions, thereby providing potential benefits on some cardiovascular diseases. (usda.gov)
The study found significant cytokine accumulation during 5 days of storage time in leukocyte contaminated platelet concentrates, suggesting that the platelet storage time has an effect on IL-2R levels. (who.int)
It has been suggested that cytokine acc leukoreduction prevents transmission of cyc cumulation in plasma of platelet concenc tomegalovirus and decreases alloimmunizac trates (PC) during storage may contribute tion to human leukocyte antigen (HLA), it to development of nonchaemolytic febrile does not prevent allergic reactions. (who.int)
We undertook this study to investigate receptor (IL-2R) accumulation is related to whether the cytokine accumulation in PCs the leukocyte content of the platelet comc obtained from single apheresis donors was ponent and can be reduced by prestorage beyond the acceptable limits of WBC conc leukocyte depletion [ 3 ]. (who.int)
P-selectin glycoprotein ligand-1 ( PSGL-1 ) is a glycoprotein found on white blood cells and endothelial cells that binds to P-selectin (P stands for platelet ), which is one of a family of selectins that includes E-selectin (endothelial) and L-selectin (leukocyte). (wikidoc.org)
We could conclude that prasugrel inhibits several markers of platelet activation and the formation of platelet-leukocyte aggregates more efficiently than clopidogrel. (avhandlingar.se)
Furthermore, in a moue model of arterial inflammation we found that P-selectin is responsible for a significant part of the platelet-leukocyte interactions. (avhandlingar.se)
Platelet aggregation, granule secretion and platelet-leukocyte interaction were analyzed by lumi-aggregometry and flow cytometry. (elifesciences.org)

It is a defect in the expression of ligands for selectins due to lack of enzymes required for expression of selectin ligands. (medscape.com)

Leukocyte rolling in postcapillary venules of inflamed tissues is reduced in L-selectin-deficient mice and mice treated with L-selectin blocking antibodies, but the glycoprotein ligand for L-selectin in inflamed venules is unknown. (rupress.org)

Currently, 18 subtypes have been reported: 12 are type I (dysfunctional lipid-linked oligosaccharide precursor synthesis), and 6 are type II (dysfunctional trimming/processing of the protein-bound oligosaccharide), including leukocyte adhesion deficiency II (CDG-IIc). (medscape.com)

Selectins play a key role in leukocyte trafficking during the inflammatory response of the organism, i.e. the recruitment and extravasation of leukocytes from the blood stream into inflamed tissue. (chimia.ch)
It also plays a role in leukocyte rolling on activated endothelial cells. (biolegend.com)

The standard product used in this kit is recombinant mouse E-Selectin, excluding intercellular E-Selectin and transmembrane domain. (innov-research.com)
The potential of recombinant ALP to block the interactions of leukocytes with the endothelial lining was concomitantly investigated in vitro and in vivo . (biomedcentral.com)

L-selectin varies between cell types, has ranging molecular weight from 65 kDa in lymphocytes to 100 kDa in neutrophils, and is due to cell type-specific glycosylation. (wikipedia.org)
L-selectin is also expressed by naive B cells, with the loss of L-selectin distinguishing activated B cells destined to differentiate to antibody-secreting cells L-selectin is expressed on circulating neutrophils and is shed following neutrophil priming. (wikipedia.org)
Expression of L-selectin in neutrophils decreases with neutrophil aging. (wikipedia.org)
In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes, and eosinophils. (southernbiotech.com)
Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes. (southernbiotech.com)
This involves activation and directed migration of leukocytes (neutrophils, monocytes and eosinophils) from the venous system to sites of damage (Box 1), and tissue mast cells also have a significant role. (weeksmd.com)
For in vivo studies, we focused on P-selectin glycoprotein ligand-1 (PSGL-1), a key adhesion molecule overexpressed in APS neutrophils. (jci.org)

The nature of the interactions between L-selectin and ligand depends on many circumstances, primarily the location of anatomically defined sites in the high vessel venules (perivascular, extravascular and intravascular). (wikipedia.org)
It is believed that adhesive interactions between selectins on the blood vessel wall and selectin ligands on the CTC surface facilitate metastatic progression, in a manner similar to the leukocyte adhesion cascade essential in the inflammatory response (3). (aiche.org)

CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. (biolegend.com)

Classical monocytes express high levels of L-selectin while in circulation. (wikipedia.org)
Shedding of L-selectin from monocytes occurs during trans-endothelial migration. (wikipedia.org)

PSGL, P-selectin glycoprotein ligand-1. (rupress.org)
Here, we show that L-selectin-dependent rolling after P-selectin blockade is completely absent in P-selectin glycoprotein ligand-1 (PSGL-1) −/− mice or wild-type mice treated with a PSGL-1 blocking monoclonal antibody. (rupress.org)
1997). "Isolated P-selectin glycoprotein ligand-1 dynamic adhesion to P- and E-selectin" . (wikidoc.org)

Leukocyte adhesion deficiency (LAD) is a rare primary immunodeficiency. (medscape.com)
Thus the infections in patients with leukocyte adhesion deficiency act similarly as those observed in patients with neutropenia. (medscape.com)
Labial ulceration from which Escherichia coli was cultured in an 8-month-old girl with leukocyte adhesion deficiency type 1 (LAD I). Note the thin bluish scar at the superior aspect of the labia from an earlier cellulitis. (medscape.com)
This 3-year-old girl had leukocyte adhesion deficiency type I (LAD I) with complete absence of CD18 expression. (medscape.com)
This 10-month-old patient with severe leukocyte adhesion deficiency type I (LAD I) developed a cervical adenitis caused by Klebsiella pneumoniae. (medscape.com)
Leukocyte adhesion deficiency type I (LAD I) is a failure to express CD18, which composes the common ß 2 subunit of LFA1 family (ß2 integrins). (medscape.com)
In milder forms of leukocyte adhesion deficiency I (1-30% expression of CD8), patients may survive to adulthood. (medscape.com)
Patients with leukocyte adhesion deficiency II manifest the Bombay phenotype (ie, negative for O and H blood group antigens with potential production of anti-H antibody). (medscape.com)
However, IgM and IgG serum levels are within the reference range in patients with leukocyte adhesion deficiency II. (medscape.com)
Leukocyte adhesion deficiency II may be classified as one of the congenital disorders of glycosylation (CDG), a rapidly expanding group of metabolic syndromes with a wide symptomatology and severity. (medscape.com)
Variants of leukocyte adhesion deficiency have also been reported, including fully expressed but nonfunctional CD18 and an E selectin that is expressed but rapidly cleaved from the cell surface (only present in soluble form). (medscape.com)
Thus, patients with this type of leukocyte adhesion deficiency manifest both severe bacterial infections and bleeding disorder. (medscape.com)
This leukocyte adhesion deficiency variant is associated with defective expression of the Rap-1 activator CalDAG-GEFI. (medscape.com)
Leukocyte adhesion deficiency results from an adhesion molecule defect that causes granulocyte and lymphocyte dysfunction and recurrent soft-tissue infections. (msdmanuals.com)
Manifestations of leukocyte adhesion deficiency usually begin in infancy. (msdmanuals.com)
Diagnosis of leukocyte adhesion deficiency is by detecting absence or severe deficiency of adhesive glycoproteins on the surface of WBCs using monoclonal antibodies (eg, anti-CD11, anti-CD18) and flow cytometry. (msdmanuals.com)
PSGL-1 deficiency was also associated with reduced leukocyte vessel wall adhesion and NET formation. (jci.org)
Rare leukocyte adhesion deficiency type II (LAD II) is due to an inborn error in fucose metabolism caused by mutations in the guanosine diphosphate-fucose transporter gene. (lu.se)

The analyzed molecules included the beta 1 integrin or VLA family VLA-1-6, the beta 2 integrins or leukocyte integrins LFA-1, Mac-1, and p150,95, the immunoglobulins LFA-3, CD2, ICAM-1 and VCAM-1 and the selectins ELAM-1 and GMP-140. (nih.gov)

This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation. (southernbiotech.com)
The monoclonal antibody MEL-14 blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling. (southernbiotech.com)

It also functions as an L-Selectin ligand and may be involved in the inhibition of stem cell differentiation and leukocyte-endothelial adhesion (1, 2). (rndsystems.com)

L-selectin belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups containing a Sialyl LewisX (sLeX) determinant. (wikipedia.org)
This disease is a defect in fucose metabolism (lack of fucosylation of the carbohydrate selectin ligands) that results in failure to express the ligand for E and P selectin, sialyl Lewis-X (CD15s) expressed on leukocytes and endothelial cells. (medscape.com)
This mini-review describes selectin antagonists, where the N-acetylneuraminic acid moiety of the natural ligand sialyl Lewisx is replaced by mimetics containing the essential carboxylic acid function. (chimia.ch)
There is a failure to express the ligand for E and P selectin, sialyl Lewis-X (CD15s), which is expressed on leukocytes . (lu.se)

Most glycoproteins undergo either N- or O-linked glycosylation, and it is very likely that the type of L-selectin glycosylation determines the specific functions of individual cells, but this has not yet been investigated in detail. (wikipedia.org)
The activation state of different PMN preparations was measured by immunofluorescence staining of the adhesion glycoproteins Mac-1 and L-selectin. (aspetjournals.org)
E-selectin (SELE) is a part of a family of divalent cation-dependent carbohydrate-binding glycoproteins or adhesion molecules. (prospecbio.com)

L-selectin acts as a "homing receptor" for lymphocytes to enter secondary lymphoid tissues via high endothelial venules. (wikipedia.org)
Ligands present on endothelial cells will bind to lymphocytes expressing L-selectin, slowing lymphocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. (wikipedia.org)
SELPLG codes for PSGL-1, the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. (wikidoc.org)

Our studies of inflammation biology examine the contribution of cell surface carbohydrate structures or glycans to white blood cell (leukocyte) activation and adhesion. (buffalo.edu)

Selectins contribute to both SSRBC and neutrophil adhesion (PMN) in vitro and in SCD mouse models. (functionalfluidics.com)

Thus, intravital fluorescence microscopic imaging of leukocyte rolling and firm adhesion to postcapillary venules were performed in the knee joints of DBA1/J mice after intravenous injection of anti-CII mAbs. (biomedcentral.com)

L-selectin is composed of multiple structural regions: an N-terminus C-type lectin domain, an adjacent epidermal growth factor-like domain, two to the consensus repeat units homologous to those found in C3/C4-binding proteins, an extracellular cleavage site, a short transmembrane domain, and a cytoplasmic tail. (wikipedia.org)
CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and epidermal growth factor-like domains. (southernbiotech.com)

The end goal is to apply systems level understanding of glycosylation processes in order to uncover new pathways and small molecule inhibitors that can control leukocyte migration during inflammation. (buffalo.edu)
Although numerous research efforts to identify small molecule selectin antagonists have been reported, no successful drug has been identified so far. (chimia.ch)

However, inflammation causes the expression of cell adhesion molecules (CAM) such as P-selectin on the surface of the blood vessel wall. (wikidoc.org)

L-selectin is also expressed by lymphoid primed hematopoietic stem cells and may participate in the migration of these stem cells to the primary lymphoid organs. (wikipedia.org)
MCP-1)), adhesion molecules (i.e., selectins and adhesion molecules (ICAM-1, VCAM-1)), and successive migration of leukocytes into the myocardium [ 12 , 13 ]. (hindawi.com)

Selectins are part of the broader family of cell adhesion molecules . (wikidoc.org)

1997. Leucocyte Typing VI. (biolegend.com)

Standards(NSO, W22-P557) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for E-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. (innov-research.com)

Because of the diversity of L-selectin ligands, signals that propagate downstream of L-selectin provide information about the position of the leukocyte within the multistep adhesion cascade (binding, rolling, adhesion, and transmigration). (wikipedia.org)

Reacts with human Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1), E-selectin. (covalab.com)

Heparins exhibit blockade of P‐selectin‐mediated adhesion. (functionalfluidics.com)

[ 1 , 2 ] The clinical picture is characterized by marked leukocytosis and localized bacterial infections that are difficult to detect until they have progressed to an extensive level secondary to lack of leukocyte recruitment at the site of infection. (medscape.com)

A monoclonal antibody from rat specific for E-Selectin has been precoated onto 96-well plates. (innov-research.com)

Multiparameter flow cytometric analysis of CD15 expression on human peripheral blood leucocyte populations. (bdbiosciences.com)

Les numérations plaquettaire et leucocytaire ainsi que la concentration en cytokines (IL-2R, IL-8 et TNF-) ont été me- surées. (who.int)

To determine leukocyte functionalization with ES/TRAIL, fluorescent (FL) liposomes were exposed to human blood under shear flow, and leukocytes were isolated and analyzed for ES/TRAIL attachment using FC and confocal microscopy. (aiche.org)
Upon exposure to shear flow, ES/TRAIL liposomes in human blood were functionalized to the surface of leukocytes, as confirmed by confocal microscopy (Fig. 1A) and FC. (aiche.org)
Exposure of functionalized ES/TRAIL leukocytes into cancer cell-spiked blood successfully targeted and killed cancer cells under shear flow, compared to controls (Fig. 1B). (aiche.org)

L-selectin expression is re-activated in cytotoxic T cells once they exit the lymph node. (wikipedia.org)
L-selectin expression is also observed on oocytes and early-stage embryos. (wikipedia.org)
An increase in L-selectin expression is observed when both the blastocyst and cytotrophoblast attach to the endometrium. (wikipedia.org)
L-selectin expression decreases by the 17th week of pregnancy, and remains low or non-existent until term (2017). (wikipedia.org)
In animal studies, mice administrated orally with caffedymine and alfrutamide (10 micro g per 35 g body weight) also showed significant reduction in the P-selectin expression by 23-34 % (P (usda.gov)
Up-regulation of leukocyte CXCR4 expression by sulfatide: an L-selectin-dependent pathway on CD4 + T cells. (southernbiotech.com)
Fluorescence-activated cell sorting was applied to determine endothelial E-selectin expression. (biomedcentral.com)
Concomitantly, ALP suppressed the IL-1β-induced NF-κB activation and the upregulation of E-selectin expression in glEND.2 cells in vitro . (biomedcentral.com)

1995. Leucocyte Typing V. Oxford University Press. (biolegend.com)

We recently reported that raised activity of the glycosylating enzyme core 2 β 1,6- N -acetylglucosaminyltransferase (GlcNAc-T) through protein kinase C (PKC)β2-dependent phosphorylation plays a fundamental role in increased leukocyte-endothelial cell adhesion and capillary occlusion in retinopathy. (diabetesjournals.org)
In this study, L-selectin was found to be phosphorylated in lymphoblastoid cell lines, and phosphorylation was enhanced by phorbol ester (phorbol 12-myristate 13-acetate (PMA)) treatment. (upenn.edu)

To investigate whether leukocyte-expressed PSGL-1 is mediating L-selectin-dependent rolling, we reconstituted lethally irradiated wild-type mice with PSGL-1 −/− bone marrow cells. (rupress.org)
In this study, a standardized flow adhesion assay was used to evaluate the effects of sevuparin on the adhesion of sickle whole blood to cultured endothelial cells and vascular cell adhesion molecule‐1 (VCAM‐1), and sickle‐leucocyte rolling adhesion on L‐selectin. (functionalfluidics.com)
The vascular adhesion molecule, E-selectin (E-sel) is responsible for the tethering and rolling of leukocytes on perivascular endothelial BM niche cells (EC). (confex.com)

The human L-selectin gene (sell) is located on the long arm of chromosome 1 (1q24.2), and is arranged in tandem with its family members (in the order: L-, P-, and E-selectin). (wikipedia.org)
Selectin P ligand , also known as SELPLG or CD162 ( cluster of differentiation 162), is a human gene . (wikidoc.org)

L-selectin, also known as CD62L, is a cell adhesion molecule found on the cell surface of leukocytes, and the blastocyst. (wikipedia.org)

Over time, these molecules are released through the process of ectodomain shedding and are replaced by newly synthesized L-selectin proteins. (wikipedia.org)

Anatomy5

Organisms2

Diseases4

Chemicals and Drugs32

Analytical, Diagnostic and Therapeutic Techniques and Equipment3

Phenomena and Processes7

Information Science1

Endothelial Leukocyte Adhesion Molecule3

Facilitating leukocyte-endothelial cell adhesion1

Platelets7

Endothelium7

Receptors7

Platelet11

Ligands for selectins1

Ligand for L-selectin1

Including leukocyte adhesion d1

Role in leukocyte2

Recombinant2

Neutrophils7

Interactions2

Type I glycoprotein1

Monocytes2

Glycoprotein ligand3

Deficiency18

Integrins1

Extravasation2

Inhibition1

Sialyl4

Glycoproteins3

Lymphocytes3

Carbohydrate1

Neutrophil1

Postcapillary venules1

Transmembrane2

Small molecule2

Blood vessel wall1

Migration2

Cell adhesion1

19971

Polyclonal antibody1

Adhesion cascade1

ELAM1

Blockade1

Recruitment1

Monoclonal antibody1

Peripheral1

Cytokines1

Shear flow3

Expression8

19951

Phosphorylation2

Rolling3

Gene2

CD62L1

Proteins1